A traditional Chinese medicine composition for reducing eye bags and its preparation method

Abstract

This invention discloses a traditional Chinese medicine composition for reducing eye bags and its preparation method. The composition, by weight, is made from the following raw materials: 100-200 parts Astragalus membranaceus, 80-150 parts Poria cocos, 80-150 parts Leonurus japonicus, 40-80 parts Salvia miltiorrhiza, 40-80 parts Panax notoginseng, 0.5-1.5 parts artificial bezoar, 2-4 parts pearl, 0.5-1.5 parts borneol, 30-70 parts 1,3-propanediol, 1-10 parts 1,2-hexanediol, and 20-70 parts water. The traditional Chinese medicine composition and its preparation method provided by this invention have few side effects, high safety, and good antioxidant, anticoagulant, and anti-aging functions, and have a definite effect on the prevention and treatment of eye bags. The preparation process is simple, easy to scale up, and has good market application prospects.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for reducing eye bags and its preparation method. Background Technology

[0002] Most women pay great attention to the appearance of the area around their eyes and are troubled by their under-eye bags. Medically, under-eye bags refer to a skin aging phenomenon characterized by loose and enlarged lower eyelid skin, typically seen in middle-aged and elderly people over 40. Histological changes in skin aging reveal vacuolation of the epidermal spinous layer, a reduction in dermal papillae, disappearance and degeneration of reticular fibers, slow regeneration of collagen fibers, decreased dermal moisture content, and reduced subcutaneous fat. These results in thinning and loosening of the skin, leading to wrinkles. The skin around the eyes is particularly thin, containing a special structure supported by the orbicularis oculi muscle, and exhibits obvious degenerative changes in the orbital septum, protruding orbital fat, and signs of aging such as loose orbicularis oculi muscle and skin.

[0003] Currently, there are many products on the market for reducing eye bags, but most have limited effectiveness. Eye bag removal options include cosmetic procedures and non-surgical treatments such as laser, radiofrequency, and ultrasound, which are slow, require multiple treatments, and are expensive. These are suitable for mild to moderate eye bags, as well as early maintenance and repair. Surgical treatments, such as external incision, internal incision, and fat dissolving eye bag removal, offer significant and lasting results, but carry certain risks. Post-operatively, there may be unevenness in the fat-dissolving area. For eye bags caused by thickened orbicularis oculi muscle, fat removal is not very effective and partial removal of the orbicularis oculi muscle is necessary. For eye bags with tear trough deformities, fat transfer or fat implantation techniques can be used to fill the hollow tear trough.

[0004] Therefore, it is necessary to develop some herbal products to reduce eye bags. Traditional Chinese medicine believes that the formation of eye bags is inseparable from blood and water. Insufficient spleen and kidney, deficiency of qi and blood, and inability of qi and blood to nourish the eyes lead to blood stasis and water retention. This obstruction of blood and water flow results in stagnation in the eyelid vessels, thus causing eye bags.

[0005] There is an urgent need to develop an eye bag fading product that is made from pure natural Chinese herbal medicines, has few side effects, is non-addictive, and has high safety, based on the basic principles of traditional Chinese medicine for fading eye bags. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to provide a traditional Chinese medicine composition for reducing eye bags and its preparation method, which has few side effects, high safety, and good antioxidant, anticoagulant, and anti-aging functions, and has a definite effect on the prevention and treatment of eye bags; the preparation process is simple and easy to scale up.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a traditional Chinese medicine composition for reducing eye bags, made from the following raw materials in parts by weight:

[0009]

[0010] As a preferred embodiment of the present invention, the herbal composition is made from the following raw materials in parts by weight:

[0011]

[0012] As a more preferred technical solution of the present invention, the herbal composition is made from the following raw materials in parts by weight:

[0013]

[0014] Secondly, the present invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0015] (1) Ethanol reflux extraction: Take Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng in proportion, add ethanol, extract and concentrate by hot reflux, and filter to obtain filtrate and residue.

[0016] (2) Concentration: After concentrating the filtrate obtained in step (1) under reduced pressure, filter it to obtain concentrated solution A for later use;

[0017] (3) Ultrasonic extraction: Add water to the filter residue obtained in step (1), extract with ultrasound, and then concentrate under reduced pressure to obtain a drug solution for later use;

[0018] (4) Alcohol precipitation concentration: Slowly add ethanol to the drug solution obtained in step (3), refrigerate and let stand, then take the supernatant, filter, and concentrate the filtrate under reduced pressure to obtain concentrated solution B.

[0019] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, and heat until the borneol is completely dissolved and the artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl and then add 1,3-propanediol, and mix well again to obtain a mixture;

[0020] (6) Filtration: After filtering the mixture obtained in step (5), the traditional Chinese medicine composition is obtained.

[0021] As a more preferred technical solution of the present invention, in step (1), the amount of ethanol is added according to the material-to-liquid ratio of 6 to 15, the ethanol concentration is 50 to 95%, the hot reflux temperature is 40 to 60°C, and the time is 0.5 to 5 hours.

[0022] As a more preferred technical solution of the present invention, in step (2), the vacuum concentration is carried out at 40-60°C and -0.06--0.08 MPa until the relative density is 1.2-1.25.

[0023] As a more preferred technical solution of the present invention, in step (3), the amount of water added is 5 to 12 times the mass of the filter residue; the ultrasonic extraction temperature is 40 to 60°C, the power is 200 to 500W, and the time is 40 to 90 minutes; the vacuum concentration is concentrated at 40 to 60°C and -0.06 to -0.08MPa to a relative density of 1.05 to 1.1.

[0024] As a more preferred technical solution of the present invention, in step (4), the ethanol concentration is 95%, and the ethanol is added to make the alcohol content 75%; it is refrigerated and left to stand for 12 to 36 hours; it is concentrated under reduced pressure at 40 to 60°C and vacuum degree ≤ -0.08MPa to make the relative density 1.15 to 1.25.

[0025] As a more preferred technical solution of the present invention, in step (5), the heating temperature is 40-50°C.

[0026] As a more preferred technical solution of the present invention, in step (6), the filtration is performed by sequentially passing the titanium rod through a microporous filter membrane of 1.0 μm and 0.60 μm.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] (1) This invention provides a traditional Chinese medicine composition for reducing eye bags, prepared from a combination of traditional Chinese medicinal materials. It has few side effects, and all raw materials meet the requirements of the "List of Used Cosmetic Raw Material Names". Among them, Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza, and Panax notoginseng are used in the formula for reducing eye bags, which conforms to the basic principles of traditional Chinese medicine for reducing eye bags, namely, invigorating qi, promoting blood circulation and diuresis. Leonurus japonicus can both invigorate blood circulation and remove blood stasis, and can also promote diuresis and reduce swelling, making its application most appropriate. Modern research shows that Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza, and Panax notoginseng all have good antioxidant, anticoagulant, and anti-aging functions, and have a definite effect on the prevention and treatment of eye bags. The high dosage of Astragalus membranaceus, Poria cocos, and Leonurus japonicus ensures the function of reducing eye bags. Pearl is replaced with pearl extract, which is easier to dissolve. At the same time, the amount of borneol is reduced to reduce its discomfort to the skin.

[0029] (2) The herbal composition of the present invention firstly regulates the microcirculation of the skin around the eyes, clears the blocked blood and qi in the eyes, and quickly eliminates eye edema, thereby reducing eye bags; the herbal composition of the present invention also exerts multiple effects of reducing eye bags by anti-aging, promoting collagen repair and enhancing the degradation of endocytic elastin.

[0030] (3) The present invention optimizes the preparation process and adopts the main process route of combining low temperature alcohol extraction and ultrasonic extraction. Low temperature alcohol extraction (temperature ≤ 60℃) shortens the heating time and greatly improves the extraction efficiency of the five raw materials of Astragalus membranaceus, Panax notoginseng, Poria cocos, Leonurus japonicus and Salvia miltiorrhiza. The extract obtained has a high content of active ingredients Tanshinone B and total saponins of Panax notoginseng. At the same time, the product obtained has good stability and quality.

[0031] (4) In this invention, 1,3-propanediol is preferably combined with 1,2-hexanediol. The preservative system formulated from these two raw materials has a shelf life of 3 years, which is safer, gentler, and more efficient than traditional preservatives, making it highly persuasive to the new generation of consumers who are concerned about green ingredients. Traditional preservatives such as parabens, formaldehyde releasers, MIT, and phenoxyethanol can irritate the skin, causing redness, swelling, and stinging; on the other hand, they can disrupt the skin's microbial balance. In addition to its antibacterial and synergistic effects, the preservative design of this invention also has moisturizing and antioxidant functions.

[0032] In summary, the herbal composition prepared by this invention has few side effects, high safety, and good antioxidant, anticoagulant, and anti-aging functions. It has a definite effect on the prevention and treatment of eye bags and has good market application prospects. Attached Figure Description

[0033] Figure 1 The difference in microartery and microvenous diameters at different time points between the blank control group and the drug-treated group.

[0034] Figure 2 To investigate the toxic effects (A) and protective concentration (B) of different concentrations of the herbal extracts for reducing eye bags on HSF cells using the CCK-8 assay. n = 5 / group, **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group.

[0035] Figure 3 The staining results (A) and the positive cell count (B) of different concentrations of the herbal extracts from Babao for reducing eye bags were studied using β-galactosidase staining to investigate their anti-HSF cell senescence effects. n = 3 / group, **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group.

[0036] Figure 4 This study investigated the effects of a traditional Chinese medicine extract for reducing under-eye bags (Babao) on the levels of type I collagen, MMP-1, MMP-3, and MMP-9 in the supernatant of photoaged HSF cells using ELISA. n = 5 / group. *P < 0.05 vs. normal control group; **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group.

[0037] Figure 5 To investigate the effects of a traditional Chinese medicine extract for reducing under-eye bags on the cell cycle of photoaged HSF cells using PI staining combined with flow cytometry. n = 3 / group, **P < 0.01 vs. normal control group; #P < 0.05 vs. model group; ##P < 0.01 vs. model group.

[0038] Figure 6 To investigate the effects of a traditional Chinese medicine extract for reducing under-eye bags on endocytosis (A) and elastin degradation (B) in photoaged HSF cells using ELISA. n = 4 / group, **P < 0.01 vs. normal control group; #P < 0.05 vs. model group. Detailed Implementation

[0039] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0040] Unless otherwise specified, all medicinal materials used in this invention were purchased through market channels. For ease of operation, the pearl in the herbal composition of this invention is added in the form of pearl extract.

[0041] Example 1

[0042] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0043]

[0044] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0045] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then 80% ethanol was added at a material-to-liquid ratio of 1:2. After hot reflux extraction and concentration at 60°C for 1.5 h, the mixture was filtered to obtain filtrate and residue.

[0046] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0047] (3) Ultrasonic extraction: Add 10 times the amount of water to the filter residue obtained in step (1), and perform ultrasonic extraction (ultrasonic temperature 60℃, ultrasonic time 60min, ultrasonic power 300W). Then concentrate the extract under reduced pressure at 50℃ and vacuum degree -0.05MPa until the relative density is about 1.1 (60℃) to obtain the drug solution for later use.

[0048] (4) Alcohol precipitation concentration: Slowly add 95% ethanol to the drug solution obtained in step (3) until the alcohol content is 70%. After refrigerating and standing for 24 hours, take the supernatant and then filter it through a 200-mesh filter. Concentrate the obtained filtrate under reduced pressure at a temperature of 50℃ and a vacuum degree of ≤-0.08MPa to a relative density of about 1.2 (60℃) to obtain concentrated solution B.

[0049] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0050] (6) Filtration: The mixture obtained in step (5) is filtered sequentially through 1.0μm and 0.60μm microporous membrane titanium rods to obtain the Babao herbal composition for lightening eye bags.

[0051] Example 2

[0052] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0053]

[0054] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0055] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then 80% ethanol was added at a material-to-liquid ratio of 1:2. After hot reflux extraction and concentration at 60°C for 1.5 h, the mixture was filtered to obtain filtrate and residue.

[0056] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0057] (3) Ultrasonic extraction: Add 10 times the amount of water to the filter residue obtained in step (1), and perform ultrasonic extraction (ultrasonic temperature 60℃, ultrasonic time 60min, ultrasonic power 300W). Then concentrate the extract under reduced pressure at 50℃ and vacuum degree -0.05MPa until the relative density is about 1.1 (60℃) to obtain the drug solution for later use.

[0058] (4) Alcohol precipitation concentration: Slowly add 95% ethanol to the drug solution obtained in step (3) until the alcohol content is 70%. After refrigerating and standing for 24 hours, take the supernatant and then filter it through a 200-mesh filter. Concentrate the obtained filtrate under reduced pressure at a temperature of 50℃ and a vacuum degree of ≤-0.08MPa to a relative density of about 1.2 (60℃) to obtain concentrated solution B.

[0059] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0060] (6) Filtration: The mixture obtained in step (5) is filtered sequentially through 1.0μm and 0.60μm microporous membrane titanium rods to obtain the Babao herbal composition for lightening eye bags.

[0061] Example 3

[0062] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0063]

[0064] In this embodiment, a method for preparing the above-mentioned traditional Chinese medicine composition is also provided, comprising the following steps:

[0065] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then 80% ethanol was added at a material-to-liquid ratio of 1:2. After hot reflux extraction and concentration at 60°C for 1.5 h, the mixture was filtered to obtain filtrate and residue.

[0066] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0067] (3) Ultrasonic extraction: Add 10 times the amount of water to the filter residue obtained in step (1), and perform ultrasonic extraction (ultrasonic temperature 60℃, ultrasonic time 60min, ultrasonic power 300W). Then concentrate the extract under reduced pressure at 50℃ and vacuum degree -0.05MPa until the relative density is about 1.1 (60℃) to obtain the drug solution for later use.

[0068] (4) Alcohol precipitation concentration: Slowly add 95% ethanol to the drug solution obtained in step (3) until the alcohol content is 70%. After refrigerating and standing for 24 hours, take the supernatant and then filter it through a 200-mesh filter. Concentrate the obtained filtrate under reduced pressure at a temperature of 50℃ and a vacuum degree of ≤-0.08MPa to a relative density of about 1.2 (60℃) to obtain concentrated solution B.

[0069] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0070] (6) Filtration: The mixture obtained in step (5) is filtered sequentially through 1.0μm and 0.60μm microporous membrane titanium rods to obtain the Babao herbal composition for lightening eye bags.

[0071] Comparative Example 1

[0072] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0073]

[0074] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0075] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Lycopus lucidus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then 80% ethanol was added at a material-to-liquid ratio of 1:2. After hot reflux extraction and concentration at 60°C for 1.5 h, the mixture was filtered to obtain filtrate and residue.

[0076] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0077] (3) Ultrasonic extraction: Add 10 times the amount of water to the filter residue obtained in step (1), and perform ultrasonic extraction (ultrasonic temperature 60℃, ultrasonic time 60min, ultrasonic power 300W). Then concentrate the extract under reduced pressure at 50℃ and vacuum degree -0.05MPa until the relative density is about 1.1 (60℃) to obtain the drug solution for later use.

[0078] (4) Alcohol precipitation concentration: Slowly add 95% ethanol to the drug solution obtained in step (3) until the alcohol content is 70%. After refrigerating and standing for 24 hours, take the supernatant and then filter it through a 200-mesh filter. Concentrate the obtained filtrate under reduced pressure at a temperature of 50℃ and a vacuum degree of ≤-0.08MPa to a relative density of about 1.2 (60℃) to obtain concentrated solution B.

[0079] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0080] (6) Filtration: The mixture obtained in step (5) is filtered sequentially through titanium rods with 1.0 μm and 0.60 μm microporous filter membranes to obtain the traditional Chinese medicine composition.

[0081] In this comparative example, Leonurus japonicus was replaced with Lycopus lucidus, while the remaining components and amounts remained the same, and the preparation method was adjusted accordingly.

[0082] Comparative Example 2

[0083] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0084]

[0085] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0086] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and 80% ethanol was added at a material-to-liquid ratio of 12. The mixture was extracted and concentrated by hot reflux at 60°C for 1.5 hours. The filtrate was collected after filtration.

[0087] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), and then filtered through a 200-mesh filter to obtain concentrated solution A;

[0088] (3) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water and concentrated solution A obtained in step (2), mix well; finally, add pearl extract (equivalent to 3g pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0089] (4) Filtration: The mixture obtained in step (3) is filtered sequentially through titanium rods with 1.0 μm and 0.60 μm microporous filter membranes to obtain the traditional Chinese medicine composition.

[0090] Compared with Example 1, Comparative Example 2 only underwent simple alcohol extraction.

[0091] Comparative Example 3

[0092] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0093]

[0094] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0095] (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then 80% ethanol was added at a material-to-liquid ratio of 1:2. After hot reflux extraction and concentration at 60°C for 1.5 h, the mixture was filtered to obtain filtrate and residue.

[0096] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0097] (3) Water decoction extraction: Add 10 times the amount of water to the filter residue obtained in step (1), heat and decoct for 1 hour each time, decoct twice, combine the decoctions, and continue to concentrate the obtained extract under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.05MPa until the relative density is about 1.1 (60℃) to obtain the medicinal liquid for later use.

[0098] (4) Alcohol precipitation concentration: Slowly add 95% ethanol to the drug solution obtained in step (3) until the alcohol content is 70%. After refrigerating and standing for 24 hours, take the supernatant and then filter it through a 200-mesh filter. Concentrate the obtained filtrate under reduced pressure at a temperature of 50℃ and a vacuum degree of ≤-0.08MPa to a relative density of about 1.2 (60℃) to obtain concentrated solution B.

[0099] (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, and heat at 50°C until the borneol is completely dissolved and the artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, and mix well again to obtain a mixture.

[0100] (6) Filtration: The mixture obtained in step (5) is filtered sequentially through titanium rods with 1.0 μm and 0.60 μm microporous filter membranes to obtain the traditional Chinese medicine composition.

[0101] Compared with Example 1, in Comparative Example 3, after alcohol extraction and water extraction, no ultrasonic treatment was performed.

[0102] Comparative Example 4

[0103] A traditional Chinese medicine composition for reducing eye bags, made from the following ingredients by weight:

[0104]

[0105] This comparative example also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0106] (1) Water decoction extraction: Take Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng according to the proportion, add 10 times the amount of water, heat and decoct for 1 hour each time, decoct twice, combine the decoction, filter and collect the filtrate and residue separately.

[0107] (2) Concentration: The filtrate obtained in step (1) is concentrated under reduced pressure at a temperature of 50℃ and a vacuum degree of -0.07MPa to a relative density of about 1.25 (60℃), filtered, and concentrated solution A is obtained for later use;

[0108] (3) Ethanol reflux extraction: Add 80% ethanol to the filter residue obtained in step (1) at a ratio of 12 times that of the material to the liquid, and extract and concentrate by hot reflux at 50°C for 1.5h. Collect the extract concentrate and continue to concentrate the extract under reduced pressure at 50°C and vacuum degree ≤-0.08MPa until the relative density is about 1.2 (60°C) to obtain concentrate B.

[0109] (4) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, heat at 50°C until borneol is completely dissolved and artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl extract (equivalent to 3g of pearl) and then add 1,3-propanediol, mix well again to obtain the mixture;

[0110] (5) Filtration: The mixture obtained in step (5) is filtered sequentially through titanium rods with 1.0 μm and 0.60 μm microporous filter membranes to obtain the traditional Chinese medicine composition.

[0111] Compared with Example 1, Comparative Example 4 was extracted with water followed by alcohol extraction.

[0112] Experimental Example 1

[0113] The total concentrate was prepared using the methods described in Example 1 and Comparative Examples 1-4. The contents of the active ingredients, total saponins of Panax notoginseng and salvianolic acid B, in the extract were determined according to the content determination methods under the Danshen and Panax notoginseng sections of the Pharmacopoeia of the People's Republic of China (2020 edition). The determination results are shown in Table 1.

[0114] Table 1

[0115] name Total saponin content of Panax notoginseng (mg / g) Danshensu B content (mg / g) Example 1 0.702 29.48 Comparative Example 1 0.707 28.77 Comparative Example 2 0.585 20.36 Comparative Example 3 0.429 16.21 Comparative Example 4 0.651 22.62

[0116] The stability of active ingredients is an important indicator of the quality of traditional Chinese medicine compositions. If the active ingredients decompose or deteriorate, it can lead to a reduction in efficacy or even adverse reactions.

[0117] The main active ingredients of the herbal composition of this invention are salvianolic acid B and total saponins of Panax notoginseng. Salvianolic acid B is a water-soluble component, while total saponins of Panax notoginseng are slightly soluble in water. Whether in an aqueous or alcoholic solution system, temperature and heating time significantly affect the stability of salvianolic acid B. Under heating conditions above 60°C, it can be hydrolyzed into tanshinone; the longer the heating time, the higher the degradation rate. However, if the temperature is too low, the solvent has difficulty penetrating the drug, resulting in low extraction efficiency. Therefore, in this embodiment of the invention, reflux extraction at 60°C is preferred. This reduces the degradation rate of salvianolic acid B while minimizing the heating time through reflux extraction. Low-temperature alcohol extraction is also considered as an effective method for extracting total saponins of Panax notoginseng.

[0118] To extract the active ingredients more fully, the filter residue is extracted again in the process of this invention. However, conventional water extraction or alcohol extraction cannot completely extract the active ingredients of Chinese medicinal materials. This invention specifically uses alcohol extraction-ultrasonic water extraction, which can better extract the two active ingredients at a relatively low temperature of no more than 60°C, without destroying or degrading salvianolic acid B.

[0119] Experimental Example 2

[0120] This experimental example uses a rat mesenteric microcirculation experiment to observe and confirm the efficacy of the herbal composition of the present invention in regulating microcirculation to reduce eye bags.

[0121] 2.1 Test Methods

[0122] The effects of drug intervention on blood flow velocity, arterial and venous diameter, and capillary volume in the mesenteric microcirculation of normal rats were observed using the in vivo mesenteric microcirculation method. This experiment was divided into a blank control group and a drug treatment group. The drug treatment group was administered the Eight Treasures Eye Bag Fading Herbal Composition (16.5 g / kg / day) from Example 1 via gavage, while the blank control group was administered an equal volume of deionized water via gavage. Gavage was performed once daily for 3 consecutive days.

[0123] On day 4 of the experiment, mice were anesthetized with sodium pentobarbital intraperitoneal anesthesia. A 3-4 cm incision was made along the midline of the abdomen, and a segment of the small intestinal mesentery from the ileocecal region was gently removed and placed on an observation tank in a 37°C constant-temperature bath. The mesentery was kept moist. The microscope height was adjusted, and one artery and one venule with an inner diameter of less than 100 μm were selected and laid flat on the observation platform in the center of the bath. A fixation plate was placed on top, and the acquired microscopic video images were analyzed in real time using a microcirculation microscope (40x) and a micro-image computer processing device. After fixing one field of view under the microscope and equilibrating for 10 minutes, the diameter and flow velocity of the arteries and venules in the selected area were observed. Then, 2 mL of 1.65 g / mL drug solution was added to the selected area, while the blank control group was added with an equal volume of physiological saline. The diameter of the arteries (A) and venules (V) in the mesenteric microcirculation of mice was observed at 10, 20, 30, and 60 minutes after drug administration. The number of open capillaries (capillary network intersection counting method) was recorded at 10 min, 20 min, 30 min and 60 min after drug administration to determine the improvement of mesenteric microcirculation in rats.

[0124] 2.2 Test Results

[0125] (1) Effects on the diameter of microarteries and venules in the mesenteric microcirculation of rats

[0126] Compared with the blank control group, the diameter of arterioles in the treatment group rats increased significantly at 30 min and 60 min after administration, with statistically significant differences (P < 0.05), as shown in Table 2. Compared with the blank control group, the diameter of venules in the treatment group rats increased significantly at 20 min, 30 min and 60 min after administration, with statistically significant differences (P < 0.05), as shown in Table 3. The results of this study indicate that the herbal composition can dilate the arterioles and venules of the mesenteric microcirculation in rats. Figure 1 ).

[0127] Table 2. Effects on the diameter of microarteries in the mesenteric microcirculation of rats ( n=8)

[0128]

[0129] Note: *P < 0.05 vs. the corresponding time in the blank control group.

[0130] Table 3. Effects on the diameter of microvenules in the mesenteric microcirculation of rats ( n=8)

[0131]

[0132] Note: *P < 0.05 vs. the corresponding time in the blank control group.

[0133] (2) Effect on the number of open intersections of the mesenteric microcirculation capillary network in rats

[0134] Compared with the blank control group, the number of open capillary network intersections in the mesenteric microcirculation of rats in the treatment group remained unchanged after administration, and the difference was not statistically significant (P < 0.01), as shown in Table 4. The results of this study indicate that the Babao Eye Bag Fading Formula has no effect on the number of open capillary network intersections in the mesenteric microcirculation of rats.

[0135] Table 4. Effects on the number of open intersections of the mesenteric microcirculation capillary network in rats ( n=8)

[0136]

[0137]

[0138] (3) Effects on blood flow patterns in the mesenteric microcirculation and microarteries of rats

[0139] Compared with the blank control group, the blood flow in the mesenteric microcirculation arteries of rats in the treatment group was significantly increased after administration, and the differences were statistically significant (see Table 5). The results of this study indicate that the Babao Eye Bag Fading Formula promotes the blood flow in the mesenteric microcirculation arteries of rats.

[0140] Table 5. Effects on blood flow patterns in the mesenteric microcirculation and microarteries of rats ( n=8)

[0141]

[0142] **P < 0.01 vs. the corresponding time in the blank control group.

[0143] In summary, the in vivo mesenteric microcirculation study results indicate that after drug administration, the diameter of microvenules and the blood flow in microarteries increased in rats, while the number of open capillary network junctions remained unchanged. This suggests that the Babao eye bag-fading formula can dilate microarteries and microvenules in the rat mesenteric microcirculation, promote microarterial blood flow, and has no effect on the opening of capillary network junctions.

[0144] Experimental Example 3

[0145] The following in vitro human fibroblast photoaging experiment was used to observe the effect of the herbal extract for reducing eye bags prepared in Example 1 at different concentrations on reducing eye bags.

[0146] 3.1 Modeling Method

[0147] HSF cells in the logarithmic growth phase were digested with trypsin, centrifuged, resuspended, and counted. They were then seeded into 96-well or 48-well plates. After adherence and growth on the second day, 8-MOP (80 ng / ml) was added and the cells were treated for 6 hours. Then, the cells were irradiated with ultraviolet light for 1 hour in a sterile laminar flow hood to prepare a skin photoaging model.

[0148] 3.2 Experimental Grouping

[0149] The study was divided into three groups: (1) Normal control group: no treatment was given; (2) Skin photoaging model group: 8-MOP and ultraviolet irradiation were used for 7 hours; (3) Eight-treasure eye bag lightening herbal extract intervention group: 8-MOP and ultraviolet irradiation were used for 7 hours, and then different concentrations of Eight-treasure eye bag lightening herbal extract were added for 48 hours.

[0150] 3.3 Detection Indicators

[0151] (1) Cell viability

[0152] At the end of the experiment, 10 μL of CCK-8 solution was added to each well, mixed by shaking, and incubated at 37℃ in a CO2 incubator for 3 hours. The absorbance (A) value of each well was measured at 450 nm using a microplate reader, and the survival rate was calculated. Survival rate (%) = (OD value of experimental group - OD value of zeroing well) / (OD value of control group - OD value of zeroing well) × 100%

[0153] (2) β-galactosidase staining

[0154] At the end of the experiment, the cells were gently washed with PBS, and fixative was added sequentially according to the kit instructions. The cells were fixed at room temperature for 15 minutes, washed twice with PBS, and then β-galactosidase staining working solution was added. The cells were incubated overnight at 37°C. The staining results of each experimental group were observed under an inverted phase-contrast optical microscope. The cytoplasm of senescent cells was stained blue. At least 500 cells were counted, and the percentage of positive cells was calculated.

[0155] (3) Type I collagen and matrix metalloproteinase levels

[0156] At the end of the experiment, cell supernatant was collected, and the levels of type I collagen and matrix metalloproteinases (MMP-1, MMP-3 and MMP-9) were measured strictly according to the ELISA kit instructions.

[0157] (4) Cell cycle

[0158] At the end of the experiment, cells were digested with trypsin, collected by centrifugation, washed twice with pre-chilled PBS, and fixed overnight at 4°C with pre-chilled 70% ethanol. Cells were collected by centrifugation, washed with PBS, resuspended in 500 μL of PBS, and incubated at 37°C for 30 min with 5 μL of RNase at a storage concentration of 10 g / L. Then, 5 μL of propidium iodide stock solution (5 g / L) was added to bring the working concentration to 50 mg / L, and the cells were stained at room temperature in the dark for 30 min. After filtering through a 200-mesh nylon mesh, DNA content was determined by flow cytometry, and cell cycle changes were analyzed.

[0159] (5) Elastin endocytosis and degradation

[0160] At the end of the experiment, complete culture medium containing 500 ng / mL soluble elastin was added, and after culturing for 48 h, the culture medium was discarded, and the cell supernatant was collected. Cells were washed once with PBS, and RIPA lysis buffer was added to collect the cells. The cells were centrifuged at 13,000 g for 15 min, and the total protein concentration was measured. The elastin (ELN) ELISA kit was used to detect the elastin concentration in the supernatant and cell lysis buffer, and the endocytosis and degradation of elastin by cells were observed. The endocytosis rate and degradation rate of elastin in HSF cells were calculated using the following formula.

[0161] Endocytosis volume = Total volume added - Content of supernatant

[0162] Endocytosis rate (%) = (Amount of cells endocytosed / Total amount added) × 100%

[0163] Degradation rate (%) = (cytotoxicity - cell lysate content) / cytotoxicity × 100%.

[0164] 3.4 Experimental Results

[0165] (1) Determination of the protective effect concentration of the herbal extracts in Babao for reducing eye bags

[0166] The herbal extracts for reducing eye bags were diluted to different concentrations, and the cell viability at each concentration was detected by CCK-8 assay. The results showed that within the concentration range of (6.3-3.2) mg / mL, there was no cytotoxic effect. Figure 2 A). The maximum concentration of 6.3 mg / mL with no toxic effect was further diluted to concentrations of 3.2, 1.6, 0.8, 0.4, and 0.2 mg / mL, and its protective effect on a skin photoaging model was observed. The results showed that compared with the normal control group, the cell survival rate in the model group was (55.26±3.71)%, and the difference was statistically significant (P<0.01). Compared with the model group, at the three concentrations of 0.2, 0.4, and 0.8 mg / mL, the cell survival rate increased to (76.06±10.91)%, (85.00±6.67)%, and (96.93±1.19)%, respectively. Figure 2 B).

[0167] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation can lead to a decrease in the survival rate of photoaged HSF cells, while the herbal extract of Babao for fading eye bags exerts a protective effect by increasing the survival rate of photoaged HSF cells.

[0168] (2) The protective effect of the herbal extracts from Babao Eye Bag Reduction on photo-aged HSF cells

[0169] β-galactosidase staining was used to detect the senescence of photoaged HSF cells. Cells were counted in groups of 500 under a light microscope. Normal cells remained unstained, while the cytoplasm of senescent cells appeared blue, representing positive staining cells. Figure 3 A). The results showed that compared with the normal control group, the percentage of positive cells in the model group was (68.33±15.65)%, and the difference was statistically significant (P<0.01). Compared with the model group, the percentages of positive cells in the low, medium, and high dose groups of the herbal extract for fading eye bags were (56.87±7.48)%, (41.53±3.30)%, and (16.53±4.36)%, respectively. Figure 3 B), and there was a statistically significant difference between the medium-dose group (P < 0.05) and the high-dose group (P < 0.01).

[0170] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation can increase the expression of intracellular β-galactosidase, leading to HSF cell aging. The herbal extract of Babao for fading eye bags inhibits photoaging HSF cell aging by reducing the expression of β-galactosidase.

[0171] (3) The repair effect of the herbal extracts of Babao for reducing eye bags on photo-aged HSF cells

[0172] The levels of type I collagen, MMP-1, MMP-3, and MMP-9 in the supernatant of photoaged HSF cells were detected by ELISA. The results showed that compared with the normal control group (7.11±1.56) ng / mL, the level of type I collagen in the model group was significantly reduced to (4.41±1.83) ng / mL, and the difference was statistically significant (P<0.05). Figure 4 A). The levels of type I collagen in the medium and high dose groups of the herbal extract for reducing eye bags were significantly increased, at (6.69±1.10) ng / mL and (8.32±0.65) ng / mL, respectively, and the difference was statistically significant.

[0173] Compared with the normal control group, the levels of MMP-1 (14.46±1.26) ng / mL, MMP-3 (20.76±3.74) ng / mL, and MMP-9 (8.21±1.73) ng / mL in the model group were significantly lower, at (7.60±1.16) ng / mL, (9.17±3.09) ng / mL, and (4.52±0.99) ng / mL, respectively, and all differences were statistically significant (P<0.01). Figure 4 B). Compared with the model group, the levels of MMP-1 in the medium and high dose groups of the Babao herbal extract for fading eye bags were significantly increased, at (11.59±2.35) ng / mL and (12.02±1.02) ng / mL, respectively, with statistically significant differences; the levels of MMP-3 in the medium and high dose groups were significantly increased, at (15.31±3.37) ng / mL and (17.81±4.41) ng / mL, respectively, with statistically significant differences; and the level of MMP-9 in the high dose group was significantly increased, at (8.04±1.06) ng / mL, with statistically significant differences.

[0174] The results of this study indicate that the combined use of 8-MOP and ultraviolet irradiation inhibits the synthesis and secretion of type I collagen, MMP-1, MMP-3 and MMP-9 in photoaged HSF cells. The herbal extracts of Babao for fading eye bags exert a skin repair effect by promoting the synthesis and secretion of type I collagen, MMP-1, MMP-3 and MMP-9 in photoaged HSF cells.

[0175] (4) Effects of Eight Treasures Herbal Extracts for Reducing Eye Bags on the Cell Cycle of Photoaged HSF Cells

[0176] The effects of the herbal extract for reducing eye bags using PI staining combined with flow cytometry on the cell cycle of photoaged HSF cells were studied. The results showed that compared with the normal control group (47.13±3.95)%, the S-phase DNA content in the model group was significantly increased (75.84±2.67)% (P<0.01). The S-phase DNA content in the low, medium, and high dose groups of the herbal extract for reducing eye bags was decreased (69.20±2.81)%, (62.33±5.66)%, and (54.44±1.65)%, respectively), and all differences were statistically significant. Figure 5 ).

[0177] The results of this study indicate that the combination of 8-MOP and ultraviolet irradiation can induce cell cycle arrest in HSF cells, mainly occurring at the S-phase arrest. The S-phase is the central link of the cell cycle, and DNA unwinds and replicates during the S-phase. After treatment with 8-MOP and ultraviolet irradiation, a large number of cells accumulate in the S-phase, which may lead to errors in DNA replication, rearrangement, and base pairing, resulting in changes in gene phenotypes. The Chinese herbal medicine extract for removing eye bags with eight treasures inhibits the S-phase arrest of photoaged HSF cells and reduces the DNA content of cells in the S-phase.

[0178] (5) Effects of the Chinese herbal medicine extract for removing eye bags with eight treasures on the endocytosis and degradation of elastin in photoaged HSF cells

[0179] The content of elastin in the lysate and supernatant of photoaged HSF cells was detected by ELISA. The total amount of elastin added to each experimental sample was 150 ng, and the endocytosis rate and degradation rate of each experimental group were calculated using the formula. The results showed that there was no significant difference in the elastin endocytosis rate among the experimental groups ( Figure 6 A). Compared with the normal control group (35.27 ± 3.17)%, the elastin degradation rate of the model group was significantly reduced, being (14.79 ± 5.21)% (P < 0.01). Compared with the model group, the elastin degradation rate of the high-dose group of the Chinese herbal medicine extract for removing eye bags with eight treasures was significantly increased, being (24.92 ± 3.29)%, and there was a statistical difference (P < 0.05, Figure 6 B).

[0180] The results of this study indicate that the combination of 8-MOP and ultraviolet irradiation-induced photoaged HSF cells has no effect on the endocytosis ability of elastin, but the degradation ability of the endocytosed elastin is reduced. The Chinese herbal medicine extract for removing eye bags with eight treasures enhances the degradation ability of photoaged HSF cells for the endocytosed elastin.

[0181] Conclusion: A photoaged HSF cell model induced by the combination of 8-MOP and ultraviolet irradiation was constructed. The cell viability decreased, the expression of intracellular β-galactosidase increased, the contents of type I collagen, MMP-1, MMP-3, and MMP-9 in the supernatant decreased, cell cycle S-phase arrest occurred, and the degradation ability of the endocytosed elastin decreased. The Chinese herbal medicine extract for removing eye bags with eight treasures plays a cell protection role by increasing cell viability, an anti-aging role by reducing the expression of intracellular β-galactosidase, a skin repair role by promoting the synthesis and secretion of type I collagen, MMP-1, MMP-3, and MMP-9, inhibits S-phase arrest, and enhances the degradation ability of the endocytosed elastin.

[0182] In summary, the Chinese herbal medicine extract for removing eye bags with eight treasures of the present invention plays a role in removing eye bags by regulating microcirculation, anti-aging, promoting collagen repair, and enhancing the degradation of the endocytosed elastin.

[0183] The applicant declares that the technical solution of this invention is illustrated through the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of individual raw materials in the product of this invention, additions of auxiliary components, and selection of specific methods, all fall within the protection scope and disclosure scope of this invention.

Claims

1. A traditional Chinese medicine composition for reducing eye bags, characterized in that, It is made from the following raw materials in parts by weight: Astragalus membranaceus 100-200 parts, Poria cocos 80-150 parts, Leonurus japonicus 80-150 parts, Salvia miltiorrhiza 40-80 parts, Panax notoginseng 40-80 parts, artificial bezoar 0.5-1.5 parts, pearl 2-4 parts, borneol 0.5-1.5 parts, 1,3-propanediol 30-70 parts, 1,2-hexanediol 1-10 parts, and water 20-70 parts; The preparation of the traditional Chinese medicine composition includes the following steps: (1) Ethanol reflux extraction: Astragalus membranaceus, Poria cocos, Leonurus japonicus, Salvia miltiorrhiza and Panax notoginseng were taken in proportion, and then ethanol was added. After hot reflux extraction and concentration, the mixture was filtered to obtain filtrate and residue. (2) Concentration: After concentrating the filtrate obtained in step (1) under reduced pressure, filter it to obtain concentrated solution A, which is ready for use; (3) Ultrasonic extraction: Add water to the filter residue obtained in step (1), extract with ultrasound, and then concentrate under reduced pressure to obtain the drug solution for later use; (4) Alcohol precipitation concentration: Slowly add ethanol to the drug solution obtained in step (3), refrigerate and let stand, take the supernatant, filter it, concentrate the filtrate under reduced pressure to obtain concentrated solution B; (5) Mixing: Mix 1,2-hexanediol, borneol and artificial bezoar, and heat until the borneol is completely dissolved and the artificial bezoar is fully dispersed; then add water, concentrated solution A obtained in step (2) and concentrated solution B obtained in step (4), and mix well; finally, add pearl and then add 1,3-propanediol, and mix well again to obtain a mixture; (6) Filtration: After filtering the mixture obtained in step (5), the traditional Chinese medicine composition is obtained.

2. The herbal composition for reducing eye bags according to claim 1, characterized in that, It is made from the following raw materials in parts by weight: Astragalus membranaceus 120-180 parts, Poria cocos 80-120 parts, Leonurus japonicus 80-120 parts, Salvia miltiorrhiza 50-70 parts, Panax notoginseng 40-60 parts, artificial bezoar 0.6-1.5 parts, pearl 2-4 parts, borneol 0.5-1.2 parts, 1,3-propanediol 40-70 parts, 1,2-hexanediol 2-8 parts, and water 30-60 parts.

3. The herbal composition for reducing eye bags according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 150 parts Astragalus membranaceus, 100 parts Poria cocos, 100 parts Leonurus japonicus, 60 parts Salvia miltiorrhiza, 50 parts Panax notoginseng, 1 part artificial bezoar, 3 parts pearl, 1 part borneol, 50 parts 1,3-propanediol, 3 parts 1,2-hexanediol, and 40 parts water.

4. The herbal composition for reducing eye bags according to claim 1, characterized in that, In step (1), the amount of ethanol is added according to the material-to-liquid ratio of 6 to 15, the ethanol concentration is 50 to 95%, the reflux temperature is 40 to 60°C, and the time is 0.5 to 5 hours.

5. The herbal composition for reducing eye bags according to claim 1, characterized in that, In step (2), the vacuum concentration is carried out at 40~60℃ and -0.06~-0.08MPa until the relative density is 1.2~1.

25.

6. The herbal composition for reducing eye bags according to claim 1, characterized in that, In step (3), the amount of water added is 5 to 12 times the mass of the filter residue; the ultrasonic extraction temperature is 40 to 60°C, the power is 200 to 500W, and the time is 40 to 90 minutes; the vacuum concentration is carried out at 40 to 60°C and -0.06 to -0.08MPa until the relative density is 1.05 to 1.

1.

7. The herbal composition for reducing eye bags according to claim 1, characterized in that, In step (4), the ethanol concentration is 95%, and ethanol is added until the alcohol content is 75%; it is refrigerated and left to stand for 12~36h; it is concentrated under reduced pressure at 40~60℃ and vacuum degree ≤-0.08MPa until the relative density is 1.15~1.

25.

8. The herbal composition for reducing eye bags according to claim 1, characterized in that, In step (5), the heating temperature is 40~50℃.

9. A traditional Chinese medicine composition for reducing eye bags according to claim 1, characterized in that, In step (6), the filtration process involves sequentially passing the material through a titanium rod with microporous membranes of 1.0µm and 0.60µm.

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