A kind of pine borer sex attractant, attractant core and preparation method and application thereof

Abstract

The present invention relates to the technical field of biological control of red pine borer, and in particular to a red pine borer sex attractant, a lure core, and a preparation method and application thereof. The present invention successfully identifies for the first time the sex pheromone components of red pine borer, including cis-11-hexadecene acetate, cis-9, trans-11-tetradecadienyl acetate, and cis-9-tetradecene acetate. Based on the sex pheromone composition, a red pine borer sex attractant and a lure core are developed through a large number of forest biological tests. The attractant and the lure core can be used in conjunction with a trap to effectively trap and kill male red pine borer moths. The attractant and the lure core can be used for monitoring and reporting the insect pest and for green prevention and control of the red pine borer, avoiding or reducing the application of chemical pesticides, thereby reducing pollution and protecting the environment, and having significant economic, social and ecological comprehensive benefits.

Description

Technical Field

[0001] The invention relates to the technical field of biological control of red pine borer, and in particular to a red pine borer sex attractant, an attractant core, and a preparation method and application thereof. Background Art

[0002] The red pine borer (Dioryctria sylvestrella Ratzeburg), a member of the genus Dioryctria Zeller in the family Pyralidae of the order Lepidoptera, is a serious trunk-boring pest of red pine (Pinus koraiensis Siebold & Zucc) and Scots pine (Pinus sylvestris var. mongolica L.). This pest is primarily found in Heilongjiang, Jilin, Liaoning, Hebei, Jiangsu, and Tibet in my country. Its larvae infest the whorls of branches, feeding on the phloem and cambium of the trunk and thick branches. This causes resin to ooze from the affected areas, which mixes with the insect's feces to form a curd outside the borehole. Infested pine trees grow abnormally, easily breaking at the affected area or withering above the affected area, leading to the death of the entire tree or crown deformity, affecting its appearance.

[0003] At present, chemical control methods are mainly used for the prevention and control of red pine borer. Red pine borer is relatively hidden in its damage. The entire larval and pupal stages are spent on damage under the bark and in the clumps of fat. Only the adult stage briefly flies outside the tree to court, mate and reproduce. In view of the hidden harmful habits of red pine borer, it will not be directly exposed to chemical agents during most of its life history. Even if it is sprayed multiple times a year, it will not achieve the ideal control effect. In addition, in the early stages of the invasion of red pine borer, the clumps of fat on the tree are extremely small and not easy to be found. From the perspective of production application, there is a need for an efficient, safe, economical and convenient technology for monitoring and controlling red pine borer populations.

[0004] Insect sex pheromones are small molecule chemical components secreted from the gonads of insects to the outside of the body. They serve as chemical signals for reproductive mating between male and female adults and are used for information exchange between individuals of the same species. The sex pheromones released by female adults have a strong ability to attract male insects of the same species and are species specific, highly effective, non-toxic, and non-polluting. At present, there are no reports in this field on sex pheromone attractant compositions based on red pine borer. We have clarified the components and proportions of sex pheromones of red pine borer through instrumental analysis, and through forest biological activity tests, we have confirmed that the sex pheromone attractant cores developed can be used for insect monitoring and large-scale trapping and prevention of red pine borer, which can meet the technical requirements and economic and social development needs of comprehensive control of red pine borer. Summary of the Invention

[0005] To address the aforementioned issue of the complete lack of known sex pheromone components for the red pine borer, the present invention provides a red pine borer sex attractant, an attractant core, a preparation method, and applications thereof, for attracting red pine borers in forests, achieving the goal of luring and killing large numbers of them and monitoring their population dynamics. This sex attractant has the advantages of strong specificity, excellent attractant efficacy, long-lasting effectiveness, and environmental friendliness. It is suitable for population monitoring and control of red pine borers and is unaffected by terrain or topography.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides a sex attractant for red pine borer, which comprises the following components in parts by weight: 1 to 50 parts of cis-11-hexadecene acetate, 0.1 to 5 parts of cis-9, trans-11-tetradecadiene acetate and 0.1 to 5 parts of cis-9-tetradecene acetate.

[0008] Preferably, the composition comprises the following components in parts by weight: 10 parts of cis-11-hexadecene acetate, 1 part of cis-9, trans-11-tetradecadiene acetate and 1 part of cis-9-tetradecene acetate.

[0009] The present invention also provides the use of the red pine borer sex attractant described in the above technical solution in attracting red pine borer.

[0010] The present invention provides an attractant core for attracting red pine borer, comprising the red pine borer sex attractant described in the above technical solution, an antioxidant, a solvent and an attractant carrier;

[0011] The attractant carrier includes a cuff-type composite rubber plug carrier or a polyethylene plastic tube carrier.

[0012] Preferably, in a single lure:

[0013] cis-11-hexadecene acetate 0.1-5 mg;

[0014] cis-9, trans-11-tetradecadienyl acetate 0.01-0.5 mg;

[0015] cis-9-tetradecene acetate 0.01~0.5mg.

[0016] Preferably, the antioxidant includes butylated hydroxyanisole or 2,6-di-tert-butylated cresol; the amount of the antioxidant added to a single lure is 5 to 20 mg.

[0017] Preferably, the solvent includes n-hexane or dichloromethane.

[0018] The present invention also provides a method for preparing the attractant core described in the above technical solution, comprising the following steps: dissolving the red pine moth attractant in a solvent, adding the attractant dropwise into an attractant carrier, and then adding an antioxidant dropwise into the carrier.

[0019] The present invention also provides the use of the lure core described in the above technical solution in attracting red pine borer in the forest.

[0020] Preferably, the application includes: using the lure core in conjunction with a trap, and setting the distance between each trap in the forest to 30 to 60 meters.

[0021] The beneficial effects of the present invention are:

[0022] The present invention successfully identified for the first time the sex pheromone components of the red pine borer, including cis-11-hexadecene acetate, cis-9, trans-11-tetradecadienyl acetate, and cis-9-tetradecene acetate, and developed red pine borer sex attractants and lures based on the above-mentioned sex pheromone compositions through a large number of forest biological tests. Combined with the use of traps, they can effectively lure and kill male red pine borer moths, can be used for red pine borer insect infestation monitoring and green prevention and control, avoid or reduce the use of chemical pesticides, thereby reducing pollution and protecting the environment, and have significant economic, social and ecological comprehensive benefits.

[0023] The present invention provides a sex attractant for red pine borer that can be used for the following purposes:

[0024] (1) It is used for population monitoring and forecasting of red pine borer in production. The sex attractant core of the present invention has extremely high biological activity in attracting male moths. During the emergence period of red pine borer, the number of trapped red pine borer is regularly counted and the adult red pine borer is monitored so that targeted prevention and control measures can be taken in time to prevent its population from breaking out and becoming a disaster. This technology is economical and practical, and greatly reduces labor costs.

[0025] (2) Used for luring and killing large numbers of red pine borers. Since the red pine borer attractant core of the present invention has high biological activity and the red pine borer larvae bore and cause damage, it is not suitable to use chemical pesticides. By using the red pine borer sex attractant to lure and kill a large number of adult insects in the woodland, the insect population density in the forest is reduced, thereby achieving the purpose of preventing and controlling the damage caused by red pine borers. In addition, the red pine borer sex attractant core can be combined with light trapping to lure and kill a large number of adult insects in the forest.

[0026] (3) Used in the control of red pine borer using disorienting technology. During the emergence and mating period of red pine borer, by arranging high density of red pine borer sex attractant cores provided by the present invention, the directional effect of the sex pheromone released by the female insect is disrupted, and the male insect cannot find the female insect and loses the opportunity to mate. Ultimately, the number of red pine borer offspring populations in the forest is reduced, and the degree of host damage is alleviated. DETAILED DESCRIPTION

[0027] The present invention provides a red pine borer sex attractant comprising the following components by weight: 1 to 50 parts of cis-11-hexadecene acetate, 0.1 to 5 parts of cis-9,trans-11-tetradecadienyl acetate, and 0.1 to 5 parts of cis-9-tetradecene acetate. In the present invention, the red pine borer sex attractant preferably comprises the following components by weight: 10 parts of cis-11-hexadecene acetate, 1 part of cis-9,trans-11-tetradecadienyl acetate, and 1 part of cis-9-tetradecene acetate. The chemical purity of the above three components is greater than 97% by weight, and the stereoisomer purity is greater than 95% by weight.

[0028] In the present invention, the CAS numbers of the sexual attractant ingredients cis-9-tetradecene acetate, cis-9, trans-11-tetradecadiene acetate, and cis-11-hexadecene acetate are 16725-53-4, 50767-79-8, and 34010-21-4, respectively. The chemical structures are as follows:

[0029] The chemical structure of cis-9-tetradecene acetate is as follows:

[0030]

[0031] The chemical structure of cis-9, trans-11-tetradecadienyl acetate is as follows:

[0032]

[0033] The chemical structural formula of cis-11-hexadecene acetate is as follows:

[0034]

[0035] The present invention also provides the use of the red pine borer sex attractant described in the above technical solution in attracting red pine borer.

[0036] The present invention also provides an attractant for attracting red pine borer, comprising the red pine borer attractant, antioxidant, solvent and carrier described in the above technical solution; the carrier comprises a cuff-type composite rubber plug carrier or a polyethylene plastic tube carrier.

[0037] In the present invention, a single lure contains: 0.1-5 mg of cis-11-hexadecene acetate; 0.01-0.5 mg of cis-9, trans-11-tetradecadiene acetate; and 0.01-0.5 mg of cis-9-tetradecene acetate.

[0038] In the present invention, the cuff-shaped composite rubber stopper carrier contains 30-70% (w / w) chlorobutyl rubber and 70-30% (w / w) natural rubber, and the polyethylene plastic tube carrier is a 1 mL polyethylene plastic tube with a cap. The cuff-shaped composite rubber stopper has an outer diameter of 9 mm at one end, an inner diameter of 6 mm, a cuff depth of 10 mm, and an overall length of 19 mm. The composite rubber stopper contains 30-70% (w / w) chlorobutyl rubber and 70-30% (w / w) natural rubber (purchased from The West Company, Phoenixville, Pa., USA). The lure carrier is soaked in analytical grade ethanol for 20-30 days, during which the ethanol is replaced three times. The treated lure carrier is air-dried and sealed in a sealed plastic bag for later use. The 1 mL polyethylene tube has an outer diameter of 8 mm, an inner diameter of 6 mm, and a height of 31 mm. It is capped and comes in white (purchased from Kartell, Italy).

[0039] In the present invention, the antioxidant preferably includes butylated hydroxyanisole or 2,6-di-tert-butylated cresol; the amount added to a single lure is preferably 5 to 20 mg. In the present invention, the solvent preferably includes n-hexane or dichloromethane.

[0040] The present invention also provides a method for preparing the lure core described in the above technical solution, comprising the following steps: dissolving the red pine borer attractant in a solvent, adding the resultant dropwise to an attractant carrier, and then adding an antioxidant dropwise to the carrier. The present invention preferably prepares the red pine borer attractant according to the components and proportions described above, and adding the resultant dropwise to the septum of the rubber stopper or directly to the polyethylene plastic tube. The specific steps include:

[0041] (1) Weigh the above-obtained attractant for red pine borer, prepare it in a certain proportion, mix it thoroughly and set aside;

[0042] (2) dissolving the obtained red pine borer sex attractant in n-hexane or dichloromethane solvent;

[0043] (3) using a syringe to drop the sex attractant solution prepared in step (2) onto the septum of the cuff-type composite rubber stopper or directly into the polyethylene plastic tube;

[0044] (4) After the solvent in the lure prepared in step (3) has evaporated, an antioxidant is added dropwise;

[0045] (5) After the solvent of the lure prepared in step (4) has completely evaporated, the lure is sealed in a sample bag made of aluminum foil and stored at a low temperature for future use.

[0046] The present invention also provides the use of the lure core described in the above technical solution in attracting red pine borer in the forest. In the present invention, the use preferably includes: using the lure core in conjunction with traps, with each trap spaced 30 to 60 meters apart in the forest.

[0047] In order to further illustrate the content of the present invention, the present invention is described in detail below with reference to the embodiments, but they should not be understood as limiting the scope of protection of the present invention.

[0048] Example 1

[0049] The attractants for C. spectabilis prepared in this embodiment all use commercially available cis-11-hexadecene acetate, cis-9, trans-11-tetradecadiene acetate, and cis-9-tetradecene acetate, all of which have a chemical purity greater than 97% and an isomeric purity greater than 95%.

[0050] Prepare the following 8 treatments according to test requirements:

[0051] Treatment 1: cis-11-hexadecene acetate 800 μg;

[0052] Treatment 2: cis-9, trans-11-tetradecadienyl acetate 800 μg;

[0053] Treatment 3: cis-9-tetradecene acetate 800 μg;

[0054] Treatment 4: 800 μg of cis-11-hexadecene acetate and 80 μg of cis-9, trans-11-tetradecadiene acetate;

[0055] Treatment 5: 800 μg of cis-11-hexadecene acetate and 80 μg of cis-9-tetradecene acetate;

[0056] Treatment 6: 400 μg of cis-9, trans-11-tetradecadienyl acetate and 400 μg of cis-9-tetradecenyl acetate;

[0057] Treatment 7: cis-11-hexadecene acetate 800 μg, cis-9, trans-11-tetradecadiene acetate 80 μg, and cis-9-tetradecene acetate 80 μg;

[0058] Treatment 8: blank control.

[0059] Mix the three compounds described above in the ratios specified in Treatments 1-7 and dissolve them in n-hexane to prepare a sex attractant solution. Next, prepare a commercially available cuff-type composite rubber lure carrier, containing 30-70% (w / w) chlorobutyl rubber and 70-30% natural rubber. Add 10 μL of the prepared sex attractant solution dropwise to the composite rubber stopper septum. Add 20 μL of the antioxidant 2,6-di-tert-butyl-p-cresol (10 mg / lure) directly to the septum. Treatment 8 consisted of only the cuff-type composite rubber stopper, solvent, and antioxidant, without any added sex attractant components. The lure prepared in Treatment 8 served as a blank control.

[0060] The bait cores are sealed in aluminum foil bags and refrigerated at 4°C. If used in the wild, it is best to transport them at low temperatures.

[0061] The application of the red pine borer sex attractant core prepared in Example 1 to attract red pine borer adults is as follows:

[0062] 1) Trap Construction: The trap is primarily a triangular viscose trap. It is made from a single piece of calcite board (length x width = 90 x 25 cm) folded together and secured with wire to form a regular triangle with a side length of 30 cm. A viscose sheet (28 x 23 cm) is placed over the bottom sheet of the trap. This sheet is replaced regularly based on the number of insects caught.

[0063] 2) Suspension of the lure: Suspend a lure in the center of the triangular viscose trap 2-3 cm above the bottom surface. The lure can be suspended with a thin wire.

[0064] 3) Field setup of traps: Each experimental treatment was replicated three times, and the traps were randomly arranged in blocks in the forest. The spacing between traps within a block was approximately 30 meters, and the spacing between traps between blocks was approximately 60 meters. The traps were hung on the main branches of the tree canopy with wire, and the traps were 3 meters above the ground.

[0065] 4) Data collection and trap emptying: Traps should be hung correctly as required when each generation of adults just emerges. Investigators should record the number of male insects attracted and the weather conditions of the day every 2-3 days. If more than 10 adults are attracted, new sticky boards should be replaced.

[0066] According to the specific usage steps mentioned above, the peak of the next generation of adult emergence can be predicted through trapping data and weather conditions, laying the foundation for the comprehensive management of red pine tip borer.

[0067] From June 17 to June 24, 2023, a moth trapping experiment was conducted in the Xinjiang Experimental Forest Farm in Fuyu County, Qiqihar City, Heilongjiang Province, using the sex attractant prepared in Example 1. The trapping results are shown in Table 1. Three compounds—cis-11-hexadecene acetate (Z11-16:OAc), cis-9, trans-11-tetradecadienyl acetate (Z9,E11-14:OAc), and cis-9-tetradecene acetate (Z9-14:OAc)—were used in the moth trapping experiment according to the seven aforementioned mixtures. Each treatment was replicated three times, and the number of moths trapped in each trap was the sum of the three traps. Depending on weather conditions, the number of moths trapped in each trap was observed every two to three days. If more than ten adults were trapped, the adhesive sheet was replaced. Analysis of insect attraction revealed that lures containing Z11-16:OAc (Treatment 1), Z9,E11-14:OAc (Treatment 2), and Z9-14:OAc (Treatment 3) alone, or a combination of any two components (Treatments 4-6), had no moth-attracting effect. However, a lure containing all three components in their proper proportions (Treatment 7) exhibited a significant moth-attracting effect. Furthermore, a blank control lure had no moth-attracting effect (Treatment 8). This suggests that the three components, Z11-16:OAc, Z9,E11-14:OAc, and Z9-14:OAc, are essential for their bioactivity against C. chinensis, and that their proportions are critical.

[0068] Table 1 Effects of different ratios of sex attractant components on moth attraction to red pine borer (June 17-24, 2023)

[0069]

[0070]

[0071] Example 2

[0072] The sex attractants for C. spectabilis prepared in this embodiment all use commercially available cis-11-hexadecene acetate and trans-11-hexadecene acetate, cis-9, trans-11-tetradecadiene acetate, cis-9-tetradecene acetate, and trans-9-tetradecene acetate, and their chemical purity is greater than 97%, and their isomer purity is greater than 95%.

[0073] Based on the biological activity test results in Example 1, the effects of the cis and trans isomers of the compound on the biological activity of Cnidium densiflorum were further analyzed. The chemical components were prepared in the following 6 treatment proportions.

[0074] Treatment 1: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0075] Treatment 2: 400 μg of cis-11-hexadecene acetate, 400 μg of trans-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0076] Treatment 3: trans-11-hexadecene acetate 800 μg, cis-9, trans-11-tetradecadiene acetate 80 μg, and cis-9-tetradecene acetate 80 μg;

[0077] Treatment 4: cis-11-hexadecene acetate 800 μg, cis-9, trans-11-tetradecadiene acetate 80 μg, and trans-9-tetradecene acetate 80 μg;

[0078] Treatment 5: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, 80 μg of cis-9-tetradecene acetate, and 80 μg of trans-9-tetradecene acetate;

[0079] Treatment 6: blank control.

[0080] The five compounds were mixed evenly in the proportions described in Treatments 1-5 and dissolved in n-hexane to prepare a sex attractant solution. A commercially available cuff-type composite rubber lure carrier, containing 30-70% (w / w) chlorobutyl rubber and 70-30% natural rubber, was then added dropwise to the composite rubber stopper septum. The antioxidant 2,6-di-tert-butyl-p-cresol (20 μL / lure) (10 mg / lure) was then added directly to the septum. Treatment 6 consisted of only the cuff-type composite rubber stopper, solvent, and antioxidant, without any added sex attractant components. The lure prepared from Treatment 6 served as a blank control.

[0081] According to the specific implementation steps in Example 1, from June 17 to June 24, 2023, another test site of Pinus sylvestris var. mongolica in Xinjiang Experimental Forest Farm, Fuyu County, Qiqihar City, Heilongjiang Province was used to prevent and control the application of red pine moth borer. The specific results are shown in Table 2. Each treatment of this test was repeated 3 times, and the number of moths trapped in each treatment was the sum of the three traps. Depending on the weather conditions, the number of moths trapped in the trap was observed every 2-3 days. If the trap was full of more than 10 male moths, the sticky glue was replaced. From the analysis of the insect trap effect, among the three component attractants with the best moth trapping effect of Z11-16: OAc, Z9, E11-14: OAc and Z9-14: OAc (treatment 1), adding E11-16: OAc component (treatment 2) or E9-14: OAc component (treatment 5) significantly reduced the moth trapping effect. Completely replacing Z11-16:OAc and Z9-14:OAc with their isomeric components, E11-16:OAc (Treatment 3) or E9-14:OAc (Treatment 4), completely suppressed the moth-attracting efficiency of the optimal lure, resulting in moth attractants consistent with those of the blank control. This indicates that the isomeric purity of the pine borer attractant significantly influences its moth-attracting efficiency. This further demonstrates that the simultaneous presence of highly pure Z11-16:OAc, Z9, E11-14:OAc, and Z9-14:OAc components is crucial for optimal moth-attracting efficiency.

[0082] Table 2 Effect of isomer combinations on moth attraction to red pine borer (June 17-24, 2023)

[0083]

[0084] Example 3

[0085] The attractants for C. spectabilis prepared in this embodiment all use commercially available cis-11-hexadecene acetate, cis-9, trans-11-tetradecadiene acetate, and cis-9-tetradecene acetate, all of which have a chemical purity greater than 97% and an isomer purity greater than 95%.

[0086] In this example, a forest bioassay was conducted at a fixed ratio of Z9, E11-14:OAc and Z9-14:OAc of 1:1 in a Pinus sylvestris var. mongolica forest at Xinjiang Experimental Forest Farm in Fuyu County, Qiqihar City, Heilongjiang Province, from August 5 to 12, 2023, to observe the effects of varying Z11-16:OAc dosages on moth attraction to the red pine borer. The following seven treatments were formulated according to the bioassay requirements.

[0087] Treatment 1: 3200 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0088] Treatment 2: 1600 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0089] Treatment 3: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0090] Treatment 4: 400 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0091] Treatment 5: cis-11-hexadecene acetate 200 μg, cis-9, trans-11-tetradecadiene acetate 80 μg, and cis-9-tetradecene acetate 80 μg;

[0092] Treatment 6: 100 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0093] Treatment 7: blank control.

[0094] The three compounds were mixed evenly in the proportions described in Treatments 1-6 and dissolved in n-hexane to prepare a sex attractant solution. A commercially available cuff-type composite rubber lure carrier, containing 30-70% (w / w) chlorobutyl rubber and 70-30% natural rubber, was then used. 10 μL of the prepared sex attractant solution was dripped onto the septum inside the cuff of the composite rubber plug. The antioxidant 2,6-di-tert-butyl-p-cresol (20 μL / lure, 10 mg / lure) was then added directly to the septum. A trap with a blank lure (Treatment 7, consisting of only the cuff-type composite rubber plug, solvent, and antioxidant, without any sex attractant components) was used as a blank control.

[0095] According to the specific implementation steps in Example 1, the application of the moth for preventing and controlling red pine moth in the Xinjiang Experimental Forest Farm in Fuyu County from August 5 to August 12, 2023 is shown in Table 3. Each treatment in this experiment was repeated 3 times, and the number of moths trapped in each treatment was the sum of the three traps. Depending on the weather conditions, the number of moths trapped in the trap was observed every 2-3 days. If more than 10 male moths were trapped in the trap, the sticky board was replaced. From the analysis of the insect trapping effect, when the three-component trap cores were fixed at a ratio of Z9, E11-14: OAc and Z9-14: OAc of 80 μg: 80 μg, the use of different doses of Z11-16: OAc trap cores could all attract male moths, among which the number of adult moths was the largest when the three components were 10:1:1 (treatment 3) or 5:1:1 (treatment 4). When the ratio deviated, the moth trapping effect decreased. Therefore, the optimal dosage of Z11-16:OAc added to the bait core of the present invention is about 400-800 μg, which can effectively and economically achieve the purpose of preventing and controlling red pine tip moth.

[0096] Table 3 Effects of Z11-16:OAc dosage changes on moth trapping in forests of red pine borer (August 5-12, 2023)

[0097]

[0098] Example 4

[0099] The attractants for C. spectabilis prepared in this embodiment all use commercially available cis-11-hexadecene acetate, cis-9, trans-11-tetradecadiene acetate, and cis-9-tetradecene acetate, all of which have a chemical purity greater than 97% and an isomer purity greater than 95%.

[0100] In this example, the effect of varying the dosage of Z9, E11-14:OAc on moth attraction was observed at a fixed ratio of Z11-16:OAc to Z9-14:OAc of 10:1. The following seven treatments were prepared as needed.

[0101] Treatment 1: 800 μg of cis-11-hexadecene acetate, 320 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0102] Treatment 2: 800 μg of cis-11-hexadecene acetate, 160 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0103] Treatment 3: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0104] Treatment 4: 800 μg of cis-11-hexadecene acetate, 40 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0105] Treatment 5: 800 μg of cis-11-hexadecene acetate, 20 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0106] Treatment 6: 800 μg of cis-11-hexadecene acetate, 10 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0107] Treatment 7: blank control.

[0108] Mix the three compounds in the same proportions as in Treatments 1-6 above and dissolve them in n-hexane to prepare a sex attractant solution. Next, take a commercially available cuff-type composite rubber lure carrier, containing 30-70% (w / w) chlorobutyl rubber and 70-30% natural rubber. Add 10 μL of the prepared sex attractant solution dropwise onto the composite rubber lure spacer. Add 20 μL / lure (10 mg / lure) of the antioxidant 2,6-di-tert-butyl-p-cresol directly to the lure. A trap with a blank lure (Treatment 7, consisting of only the cuff-type composite rubber lure, solvent, and antioxidant, without any sex attractant components) serves as a blank control.

[0109] According to the specific implementation steps in Example 1, from August 12 to August 19, 2023, another experimental forest of Pinus sylvestris var. mongolica was used to prevent and control the application of red pine moth moth in Xinjiang Experimental Forest Farm, Fuyu County. The specific results are shown in Table 4. Each treatment in this test was repeated 3 times, and the number of moths trapped in each treatment was the sum of the three traps. Depending on the weather conditions, the number of moths trapped in the trap was observed every 2-3 days. If the number of male moths in the trap exceeded 10, the sticky board was replaced. From the analysis of the insect attractant effect, different proportions of the three components can attract male moths of red pine moth moth, but the three components have the best moth attracting effect at a ratio of 10:1:1 (treatment 3) or 10:0.5:1 (treatment 4). From the perspective of prevention and economy, a 40-80 μg dose of cis-9, trans-11-tetradecadiene acetate can be selected as the optimal dosage of the present invention.

[0110] Table 4: Effects of OAc content changes on the effectiveness of attracting moths to the forest of Pinus densiflora (August 12-19, 2023)

[0111]

[0112] Example 5

[0113] The attractants for C. spectabilis prepared in this embodiment all use commercially available cis-11-hexadecene acetate, cis-9, trans-11-tetradecadiene acetate, and cis-9-tetradecene acetate, all of which have a chemical purity greater than 97% and an isomer purity greater than 95%.

[0114] In this example, the ratio of Z11-16:OAc to Z9,E11-14:OAc was fixed at 10:1, and the effect of varying the content of Z9-14:OAc on moth attraction was observed. The following seven treatments were prepared as needed.

[0115] Treatment 1: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 320 μg of cis-9-tetradecene acetate;

[0116] Treatment 2: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 160 μg of cis-9-tetradecene acetate;

[0117] Treatment 3: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 80 μg of cis-9-tetradecene acetate;

[0118] Treatment 4: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 40 μg of cis-9-tetradecene acetate;

[0119] Treatment 5: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 20 μg of cis-9-tetradecene acetate;

[0120] Treatment 6: 800 μg of cis-11-hexadecene acetate, 80 μg of cis-9, trans-11-tetradecadiene acetate, and 10 μg of cis-9-tetradecene acetate;

[0121] Treatment 7: blank control.

[0122] Mix the three compounds in the same proportions as in Treatments 1-6 above and dissolve them in n-hexane to prepare a sex attractant solution. Next, prepare a commercially available cuff-type composite rubber lure carrier, containing 30-70% (w / w) chlorobutyl rubber and 70-30% natural rubber. Add 10 μL of the prepared sex attractant solution dropwise onto the composite rubber stopper septum. Add 20 μL / 10 mg of the antioxidant 2,6-di-tert-butyl-p-cresol directly to the prepared lure. A trap with a blank lure (Treatment 7, consisting of only the cuff-type composite rubber stopper, solvent, and antioxidant, without any sex attractant components) was used as a blank control.

[0123] According to the specific implementation steps in Example 1, the third experimental forest of Pinus sylvestris var. mongolica in Xinjiang Experimental Forest Farm, Fuyu County, was used to prevent and control the red pine moth from August 15 to August 21, 2023. The specific results are shown in Table 5. Each treatment in this experiment was repeated 3 times, and the number of moths trapped in each treatment was the sum of the three traps. Depending on the weather conditions, the number of moths trapped in the trap was observed every 2-3 days. If the number of male moths trapped in the trap exceeded 10, the sticky board was replaced. From the analysis of the insect attractant effect, the three sex pheromone components still had the best insect attractant effect when they were in a ratio of 10:1:1 (treatment 3) or 10:1:0.5 (treatment 4). Other ratios that deviate from these two ratios also have good insect attractant effects. From the perspective of bioassay effect and economic cost, the present invention selects 40-80 μg of cis-9-tetradecene acetate as the optimal dosage to achieve efficient and economical prevention and control purposes.

[0124] Table 5Z9-14: Effects of OAc content changes on moth trapping in forests of red pine borer (August 15-21, 2023)

[0125]

[0126] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A sex attractant for red pine borer, characterized in that The following masses of components are included: cis-11-hexadecene acetate 800µg, cis-9,trans-11-tetradecadiene acetate 80µg, and cis-9-tetradecene acetate 80µg.

2. Use of the red pine borer sex attractant according to claim 1 in attracting red pine borer.

3. A bait for attracting red pine borer, characterized in that: The invention comprises the red pine borer sex attractant according to claim 1, an antioxidant, a solvent and a carrier; The carrier includes a cuff-type composite rubber plug carrier or a polyethylene plastic tube carrier.

4. The lure according to claim 3, wherein: The antioxidant includes butylated hydroxyanisole or 2,6-di-tert-butylated cresol; The amount added to a single lure is 5 to 20 mg.

5. The lure according to claim 3, characterized in that: The solvent includes n-hexane or dichloromethane.

6. A method for preparing the lure according to any one of claims 3 to 5, characterized in that: The following steps are involved: The red pine borer sex attractant is dissolved in a solvent and then added dropwise into a carrier together with an antioxidant.

7. Use of the bait according to any one of claims 3 to 5 for attracting red pine borer in forests.

8. The use according to claim 7, characterized in that The application includes: using the lure core in conjunction with traps, with the interval between each trap being 30 to 60 meters.

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