Highly effective anti-hair loss composition, method for preparing the same and use thereof

Abstract

The present application provides a kind of high-efficiency anti-hair loss composition and its preparation method and use.The anti-hair loss composition includes hydrolyzed soybean protein and adenosine triphosphate disodium;Wherein, with the total mass of anti-hair loss composition as 100%, the content of the hydrolyzed soybean protein is 10-90%, and the content of the adenosine triphosphate disodium is 10-90%.The present application uses hydrolyzed soybean protein to compound adenosine triphosphate disodium, which can further synergize with hydrolyzed soybean protein to enhance cell activity, improve hair follicle health, and thus achieve the effect of effectively improving hair loss caused by hair follicle damage and promoting hair growth.

Description

Technical Field

[0001] This invention relates to a highly effective anti-hair loss and hair growth composition, its preparation method and uses, belonging to the field of daily chemical technology. Background Technology

[0002] Hair loss is a distressing problem, and the number of people experiencing it is increasing, leading to greater public awareness and concern. Hair follicles on the scalp are the sites of hair growth, providing nutrients for hair development. Under normal follicle function and structure, hair growth can be broadly divided into the anagen (growth) phase, catagen (transitional) phase, and telogen (resting) phase, with hair repeatedly growing and falling out according to the follicle cycle. When scalp hair follicles are affected by factors such as androgens, insufficient nutrient supply, and external stimuli, inflammation, follicle cycle disorders, and even follicle miniaturization can occur, resulting in significant hair loss. Generally, human hair follicles cannot regenerate; therefore, follicle growth and apoptosis have a significant impact on hair growth and loss.

[0003] Soy protein has significant biological activity and high nutritional value. When used in hair loss prevention and hair growth products, it can protect hair follicles, accelerate cell growth, and promote protein synthesis. However, due to its large molecular weight, it is difficult for the skin to absorb. Generally, it is used in products as hydrolysate of soy protein, and its efficacy is not particularly outstanding at present.

[0004] Chinese patent CN103462844A discloses a hair loss prevention compound containing hydrolyzed soy protein, which can help patients restore the normal ratio of the hair growth phase and resting phase, reduce hair loss, and promote the formation of healthy hair and scalp. However, on the one hand, the composition contains many active ingredients, making its preparation relatively complex; on the other hand, the average molecular weight of the hydrolyzed soy protein used in this invention is relatively large, making it difficult to penetrate the skin and affecting the efficacy of the hydrolyzed soy protein. Summary of the Invention

[0005] The problem the invention aims to solve

[0006] The purpose of this invention is to address the deficiencies and shortcomings of existing technologies. This invention first provides a hair loss prevention and hair growth composition containing hydrolyzed soy protein. By combining hydrolyzed soy protein and disodium adenosine triphosphate, this invention can synergistically promote the proliferation and differentiation of hair follicle cells, provide energy for cell metabolism, accelerate the restoration of hair follicle vitality, and improve hair follicle health, thereby achieving excellent hair loss prevention and hair growth effects. Furthermore, it has low irritation, good water solubility, and is easy to add and apply.

[0007] Furthermore, the present invention also provides a method for preparing a hair loss prevention and hair growth composition. The hair loss prevention and hair growth composition prepared by this method has better bioavailability and stability. The preparation method is simple and easy to implement, the raw materials are easy to obtain, and it is suitable for large-scale production.

[0008] Furthermore, the present invention also provides a hair loss prevention and hair growth scalp essence containing a hair loss prevention and hair growth composition, which can effectively repair hair follicle damage, promote hair follicle cell proliferation and differentiation, and restore hair follicle vitality, thereby achieving excellent hair loss prevention and hair growth effects.

[0009] Solution for solving the problem

[0010] This invention provides a hair loss prevention and hair growth composition comprising hydrolyzed soy protein and disodium adenosine triphosphate; wherein, based on the total mass of the hair loss prevention and hair growth composition as 100%, the content of the hydrolyzed soy protein is 10-90%, and the content of the disodium adenosine triphosphate is 10-90%.

[0011] According to the hair loss prevention and hair growth composition of the present invention, the mass ratio of the hydrolyzed soy protein to the disodium adenosine triphosphate is 1:(0.25-4); preferably, the mass ratio of the hydrolyzed soy protein to the disodium adenosine triphosphate is 1:(0.4-2.5).

[0012] According to the hair loss prevention and hair growth composition of the present invention, the hydrolyzed soybean protein is obtained by hydrolysis using a protease; preferably, the protease is an alkaline protease.

[0013] According to the hair loss prevention and hair growth composition of the present invention, the method for preparing the hydrolyzed soybean protein includes the following steps:

[0014] Mix soy protein and water evenly to obtain a premix;

[0015] After adjusting the pH of the premix, a protease was added to the premix to carry out a hydrolysis reaction, and the hydrolysis product was obtained.

[0016] The hydrolysate was freeze-dried to obtain hydrolyzed soybean protein.

[0017] According to the hair loss prevention and hair growth composition of the present invention, the amount of the protease is 1-2.5% based on 100% of the total mass of the soybean protein; and / or,

[0018] The pH value of the premix is ​​adjusted to be greater than 7, preferably 8.0-10.5; and / or,

[0019] The hydrolysis reaction takes 3-6 hours and is carried out at a temperature of 50-65°C.

[0020] According to the hair loss prevention and hair growth composition of the present invention, before freeze-drying, the composition further includes the steps of ultrasonic treatment of the hydrolysate, enzyme inactivation and sterilization, solid-liquid separation and collection of supernatant; preferably, the ultrasonic treatment time is 10-40 min.

[0021] The present invention also provides a method for preparing a hair loss prevention and hair growth composition according to the present invention, which includes the step of mixing the components of the hair loss prevention and hair growth composition.

[0022] The present invention also provides the use of the anti-hair loss and hair growth composition according to the present invention in the preparation of scalp care products; preferably, the scalp care products include one or more of scalp serum, conditioner, and hair mask; the hair cleaning products include shampoo.

[0023] The present invention also provides a scalp serum comprising the hair loss prevention and hair growth composition according to the present invention; preferably, the content of the hair loss prevention and hair growth composition is 0.01-5% based on the total mass of the scalp serum as 100%.

[0024] According to the scalp serum of the present invention, the scalp serum further comprises one or more of the following: a moisturizer, a preservative, a chelating agent, a pH adjuster, and a solvent; preferably, based on 100% of the total mass of the scalp serum, the content of the moisturizer is 3-10%, the content of the preservative is 0.1-1%, the content of the chelating agent is 0.02-0.1%, the content of the pH adjuster is 0-2%, and the content of the solvent is 81.9-96.87%.

[0025] The effects of the invention

[0026] This invention uses hydrolyzed soybean protein combined with disodium adenosine triphosphate to further synergistically enhance cell activity and improve hair follicle health, thereby effectively improving hair loss caused by hair follicle damage and promoting hair growth.

[0027] In addition, the hair loss prevention and hair growth composition involved in this invention also has the characteristics of low irritation, good permeability, high stability, good water solubility, and easy addition and application.

[0028] Furthermore, the hair loss prevention and hair growth composition prepared by the method has better bioavailability and stability. The preparation method is simple and easy to implement, the raw materials are easy to obtain, and it is suitable for large-scale production.

[0029] Furthermore, the anti-hair loss and hair growth scalp essence of the present invention contains an anti-hair loss and hair growth composition, which can effectively repair hair follicle damage, promote hair follicle cell proliferation and differentiation, and restore hair follicle vitality, thereby achieving excellent anti-hair loss and hair growth effects. Attached Figure Description

[0030] Figure 1 A comparative graph showing the test results of the stability of the adenosine triphosphate disodium of the present invention is shown;

[0031] Figure 2The diagram shows a comparison of the effects of the scalp serum in Application Example 3 of the present invention before use, after 2 months of use, and after 4 months of use. Detailed Implementation

[0032] Various exemplary embodiments, features, and aspects of the present invention will be described in detail below. The term "exemplary" as used herein means "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as superior to or better than other embodiments.

[0033] Furthermore, to better illustrate the present invention, numerous specific details are set forth in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In other instances, methods, means, apparatus, and steps well known to those skilled in the art have not been described in detail in order to highlight the spirit of the present invention.

[0034] Unless otherwise stated, all units used in this specification are international standard units, and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors that are unavoidable in industrial production.

[0035] In this specification, the word "may" has two meanings: to perform a certain process and not to perform a certain process.

[0036] In this specification, references to "some specific / preferred embodiments," "other specific / preferred embodiments," "implementation," etc., refer to specific elements (e.g., features, structures, properties, and / or characteristics) related to that embodiment, which are included in at least one of the embodiments described herein and may or may not be present in other embodiments. Furthermore, it should be understood that these elements may be combined in any suitable manner in various embodiments.

[0037] In this specification, the range of values ​​referred to as "value A to value B" refers to the range including the endpoint values ​​A and B.

[0038] In this instruction manual, when "room temperature" or "room temperature" is used, the temperature can be 15-25℃.

[0039] <First Aspect>

[0040] A first aspect of the present invention provides a hair loss prevention and hair growth composition comprising hydrolyzed soy protein and disodium adenosine triphosphate; wherein, based on the total mass of the hair loss prevention and hair growth composition being 100%, the content of the hydrolyzed soy protein is 10-90%, and the content of the disodium adenosine triphosphate is 10-90%.

[0041] This invention utilizes hydrolyzed soy protein combined with disodium adenosine triphosphate (ATP) to further synergistically enhance cell activity and improve hair follicle health, thereby effectively improving hair loss caused by hair follicle damage and promoting hair growth. Furthermore, the anti-hair loss and hair growth composition involved in this invention also features low irritation, good permeability, high stability, good water solubility, and ease of application.

[0042] The mass ratio of hydrolyzed soy protein to disodium adenosine triphosphate (ATP) is 1:(0.25-4), for example: 1:0.5, 1:0.8, 1:1, 1:1.2, 1:1.5, 1:1.8, 1:2, 1:2.2, 1:2.5, 1:2.8, 1:3, 1:3.2, 1:3.5, 1:3.8, etc.; preferably, the mass ratio of hydrolyzed soy protein to disodium ATP is 1:(0.4-2.5). When the mass ratio of hydrolyzed soy protein to disodium ATP is 1:(0.25-4), it can promote hair growth; when the mass ratio of hydrolyzed soy protein to disodium ATP is 1:(0.4-2.5), the effect of promoting hair growth is even better.

[0043] The hair loss prevention and hair growth composition of the present invention is a dry powder solid, which can greatly avoid the hydrolysis of adenosine triphosphate disodium, improve the stability of the hair loss prevention and hair growth composition, and make the hair loss prevention and hair growth composition easy to use.

[0044] Hydrolyzed soy protein

[0045] Excessive oxygen free radicals can damage the mitochondrial function of cells within hair follicles, leading to cell aging and death, which in turn damages the hair follicles and causes hair loss. Hydrolyzed soy protein's significant antioxidant properties can protect hair follicle cells and reduce hair loss caused by follicle damage. Simultaneously, its amino acids help accelerate the synthesis of extracellular matrix proteins, providing cells with essential metabolic components, increasing the volume and length of hair follicles, and promoting hair growth. Furthermore, the abundant amino acids in hydrolyzed soy protein can interact with disulfide bonds in hair, providing a certain degree of protection for the hair.

[0046] However, the molecular weight of existing commercially available hydrolyzed soy protein is relatively high, which is not conducive to absorption. The hydrolyzed soy protein prepared by the method of this invention has higher activity and lower molecular weight when used in anti-hair loss and hair growth compositions, which can greatly enhance the effect of hydrolyzed soy protein in repairing hair follicle damage and promoting hair follicle cell proliferation and differentiation.

[0047] In some specific embodiments, in this invention, the hydrolyzed soy protein is obtained by hydrolysis using a protease; preferably, the protease is an alkaline protease. The inventors of this invention have discovered that using an alkaline protease can yield more peptides and amino acids with smaller molecular weights, which improves the permeability of the composition and allows the hydrolyzed soy protein to exert its repairing and nourishing effects on hair follicles to a greater extent.

[0048] In some specific implementations, the method for preparing hydrolyzed soybean protein includes the following steps:

[0049] Mix soy protein and water evenly to obtain a premix;

[0050] After adjusting the pH of the premix, a protease was added to the premix to carry out a hydrolysis reaction, and the hydrolysis product was obtained.

[0051] The hydrolysate was freeze-dried to obtain hydrolyzed soybean protein.

[0052] Specifically, based on the total mass of the soy protein as 100%, the amount of protease used is 1-2.5%, for example: 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, 2.1%, 2.2%, 2.3%, 2.4%, etc. When the amount of protease is 1-2.5%, the desired hydrolyzed soy protein can be obtained. If the amount of protease is too low, the degree of hydrolysis of the soy protein will be insufficient, resulting in a hydrolysate with an excessively high molecular weight, which is not conducive to absorption. If the amount of protease is too high, on the one hand, further increasing the enzyme dosage will not significantly increase the degree of hydrolysis of the soy protein; on the other hand, it will also lead to a decrease in the yield of small molecule peptides, resulting in more amino acids in the hydrolysate, thereby impairing the efficacy of the product.

[0053] Specifically, the present invention preferably uses alkaline protease for the hydrolysis reaction. Therefore, during the hydrolysis reaction, it is generally necessary to adjust the pH of the premix to the optimal pH value for alkaline protease. Specifically, the pH value of the premix is ​​adjusted to be greater than 7, preferably 8.0-10.5, for example: 8.2, 8.4, 8.6, 8.8, 9, 9.2, 9.4, 9.6, 9.8, 10, 10.2, 10.4, etc.

[0054] The hydrolysis reaction takes 3-6 hours, for example: 3.2h, 3.5h, 3.8h, 4h, 4.2h, 4.5h, 4.8h, 5h, 5.2h, 5.5h, 5.8h, etc.; the hydrolysis reaction temperature is 50-65℃, for example: 51℃, 52℃, 53℃, 54℃, 55℃, 56℃, 57℃, 58℃, 59℃, 60℃, 61℃, 62℃, 63℃, 64℃, etc. When the hydrolysis reaction takes 3-6 hours and the temperature is 50-65℃, the hydrolysis reaction proceeds more effectively.

[0055] In this invention, the soy protein used generally has a protein content of over 80%. Soy protein can be obtained either through preparation or by purchase.

[0056] Furthermore, before freeze-drying, the invention includes steps of ultrasonic treatment of the hydrolysate, enzyme inactivation and sterilization, solid-liquid separation, and collection of the supernatant. The inventors of this invention have discovered that ultrasonic treatment can reduce the molecular weight of the hydrolysate, thereby obtaining hydrolyzed soybean protein with superior efficacy. Preferably, the ultrasonic treatment time is 10-40 minutes, for example, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, etc.

[0057] In this invention, based on the total mass of the anti-hair loss and hair growth composition as 100%, the content of hydrolyzed soy protein is 10-90%, for example: 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, etc. When the content of hydrolyzed soy protein is 10-90%, the efficacy of hydrolyzed soy protein can be effectively exerted.

[0058] Disodium triphosphate

[0059] Sodium adenosine triphosphate (ATP) is a nucleotide derivative and a major source of energy in the body, participating in the metabolism of fats, proteins, sugars, nucleic acids, and nucleotides. In the composition described in this patent, sodium adenosine triphosphate is mainly used to synergistically promote the proliferation and differentiation of hair follicle cells by hydrolyzing soy protein, and to provide energy for cell metabolism, accelerating the restoration of hair follicle vitality, thereby achieving a highly effective effect in preventing hair loss and promoting hair growth.

[0060] The inventors of this invention have discovered that there is a synergistic effect between disodium adenosine triphosphate and hydrolyzed soybean protein, resulting in a highly effective effect in preventing hair loss and promoting hair growth.

[0061] In this invention, based on the total mass of the anti-hair loss and hair growth composition as 100%, the content of adenosine triphosphate disodium is 10-90%, for example: 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, etc. When the content of adenosine triphosphate disodium is 10-90%, the efficacy of adenosine triphosphate disodium can be effectively exerted.

[0062] Preparation method

[0063] The present invention also provides a method for preparing a hair loss prevention and hair growth composition according to the present invention, which includes the step of mixing the components of the hair loss prevention and hair growth composition.

[0064] The hair loss prevention and hair growth composition of this invention is a direct mixture of disodium adenosine triphosphate (ATP) and hydrolyzed soy protein without the need for solvents. It can be formulated into a scalp serum for use. Specifically, the mixture of disodium ATP and hydrolyzed soy protein can be stored in a brown bottle in a dry place below 25°C to prevent hydrolysis of the disodium ATP. In use, the hair loss prevention and hair growth composition is mixed with other ingredients, such as solvents, moisturizers, chelating agents, pH adjusters, and preservatives, to form a scalp serum. Specifically, a pH adjuster can be directly mixed with the hair loss prevention and hair growth composition to obtain a solid component. Then, the moisturizer, chelating agent, and preservative are dissolved in a solvent to form a liquid component. The solid and liquid components are then mixed to form the scalp serum for use.

[0065] <Second aspect>

[0066] A second aspect of the present invention provides the use of an anti-hair loss and hair growth composition in the preparation of scalp care products or hair cleaning products; preferably, the scalp care product includes one or more of scalp serum, conditioner, and hair mask; the hair cleaning product includes shampoo.

[0067] Furthermore, a second aspect of the present invention provides a scalp serum comprising the hair loss prevention and hair regrowth composition according to the present invention; preferably, based on 100% of the total mass of the scalp serum, the content of the hair loss prevention and hair regrowth composition is 0.01-5%, for example: 0.05%, 0.2%, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.8%, 4%, 4.2%, 4.5%, 4.8%, etc. When the content of the hair loss prevention and hair regrowth composition is 0.01-5%, its hair loss prevention and hair regrowth effect can be achieved.

[0068] In some specific embodiments, the scalp serum further includes one or more of the following: moisturizer, preservative, chelating agent, pH adjuster, and solvent. Preferably, based on 100% of the total mass of the scalp serum, the moisturizer content is 3-10%, for example: 4%, 5%, 6%, 7%, 8%, 9%, etc. The preservative content is 0.1-1%, for example: 0.2%, 0.4%, 0.6%, 0.8%, etc. The chelating agent content is 0.02-0.1%, for example: 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, etc. The pH adjuster has a content of 0-2%, for example: 0.2%, 0.4%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, etc.; using the pH adjuster can adjust the pH of the scalp serum to 5-6.5, which is beneficial to the efficacy of the anti-hair loss and hair growth composition. The solvent has a content of 81.9-96.87%, for example: 82%, 84%, 86%, 88%, 90%, 92%, 94%, 96%, etc.

[0069] Specifically, the pH adjuster comprises one or more combinations selected from citric acid, sodium citrate, potassium hydroxide, sodium hydroxide, triethanolamine, aminomethylpropanol, and arginine. The humectant comprises one or more combinations selected from glycerin, diglyceride, glyceryl glucoside, propylene glycol, 1,3-propanediol, butylene glycol, panthenol, sorbitol, betaine, sodium PCA, and hydroxyethyl urea. The preservative comprises one or more combinations selected from phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, methylparaben, p-hydroxyacetophenone, and 1,2-hexanediol. The chelating agent comprises disodium EDTA or tetrasodium EDTA.

[0070] In this invention, in order to prevent the hydrolysis of adenosine triphosphate disodium, the scalp essence of this invention can be prepared and used immediately, or the anti-hair loss and hair growth composition can be stored separately from other components. Preferably, the scalp essence is obtained by preparing and using it immediately.

[0071] Specifically, in use, moisturizers, chelating agents, and preservatives can be dissolved in a solvent to form a liquid component, while pH adjusters can be mixed with the anti-hair loss and hair growth composition to form a solid component. The liquid and solid components can be mixed to form a scalp serum that can be applied directly to the scalp.

[0072] Furthermore, to maximize the protection of the activity of the anti-hair loss and hair growth composition while ensuring ease of use, this invention allows the pH adjuster to be mixed with the anti-hair loss and hair growth composition to form a solid component for storage. Alternatively, the moisturizer, chelating agent, and preservative can be dissolved in a solvent to form a liquid component for storage.

[0073] Example

[0074] The embodiments of the present invention will be described in detail below with reference to examples. However, those skilled in the art will understand that the following examples are for illustrative purposes only and should not be considered as limiting the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer are followed. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0075] Example 1

[0076] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0077] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0078] 2) Adjust the pH to 9.0, and add 1.5% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0079] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0080] 4) Mix the prepared hydrolyzed soybean protein with disodium adenosine triphosphate in a 1:4 ratio and then put it into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0081] Example 2

[0082] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0083] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0084] 2) Adjust the pH to 8.5, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0085] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0086] 4) Mix the prepared hydrolyzed soybean protein with disodium adenosine triphosphate in a ratio of 3:7 and then put it into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0087] Example 3

[0088] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0089] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0090] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0091] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0092] 4) Mix the prepared hydrolyzed soybean protein with disodium adenosine triphosphate in a ratio of 2:3 and then put it into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0093] Example 4

[0094] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0095] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0096] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 55°C for 4 hours;

[0097] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0098] 4) Mix the prepared hydrolyzed soybean protein and disodium adenosine triphosphate in a 1:1 ratio and then put them into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0099] Example 5

[0100] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0101] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0102] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 5 hours;

[0103] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0104] 4) Mix the prepared hydrolyzed soybean protein with disodium adenosine triphosphate in a ratio of 3:2 and then put it into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0105] Example 6

[0106] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0107] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0108] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0109] 3) After the reaction is complete, the mixture is sonicated for 25 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0110] 4) Mix the prepared hydrolyzed soybean protein and disodium adenosine triphosphate in a ratio of 7:3 and put them into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0111] Example 7

[0112] The preparation method of the anti-hair loss and hair growth composition is as follows:

[0113] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0114] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0115] 3) After the reaction is complete, the mixture is sonicated for 20 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0116] 4) Mix the prepared hydrolyzed soybean protein with disodium adenosine triphosphate in a ratio of 4:1 and put it into a brown bottle to obtain the hair loss prevention and hair growth composition. Store it in a dry place at 5°C for later use.

[0117] Comparative Example 1

[0118] Sodium adenosine triphosphate can be used as a hair loss prevention and hair growth agent.

[0119] Comparative Example 2

[0120] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0121] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0122] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soybean protein.

[0123] Use the hydrolyzed soy protein, store it in a brown bottle in a dry place at 5°C for later use.

[0124] Comparative Example 3

[0125] Take commercially available hydrolyzed soybean protein with an average molecular weight of 15000 Da and disodium adenosine triphosphate in a 2:3 ratio, mix them evenly, and then store them in a brown bottle to obtain a hair loss prevention and hair growth composition. Store it in a dry place at 5℃ for later use.

[0126] Comparative Example 4

[0127] 1) Weigh 1g of soy protein and add an appropriate amount of distilled water and mix well;

[0128] 2) Adjust the pH to 9.0, add 2% alkaline protease (Sigma) based on the total mass of the soybean protein as 100%, and react at 50°C for 4 hours;

[0129] 3) After the reaction is complete, the mixture is sonicated for 30 minutes to inactivate enzymes and sterilize. The supernatant is then filtered and freeze-dried to obtain hydrolyzed soy protein.

[0130] 4) Mix the prepared hydrolyzed soybean protein and adenosine in a 2:3 ratio and put them into a brown bottle to obtain the anti-hair loss and hair growth composition. Store it in a dry place at 5°C for later use.

[0131] Stability test of disodium adenosine triphosphate

[0132] The stability of adenosine triphosphate disodium (ATP) was determined by testing the peak values ​​of ATP disodium under different storage conditions using high-performance liquid chromatography (HPLC). The test method involved injecting 10 μL of blank solution, standard solution, and sample filtrate into the HPLC chromatograph. Retention time and UV spectra were used to determine whether ATP disodium decomposed. The test results are shown below. Figure 1 As shown. Among them,

[0133] Blank solution: Deionized water;

[0134] Standard solution: Prepare a 300 ppm aqueous solution of disodium adenosine triphosphate standard;

[0135] Sample filtrate 1: A 1% aqueous solution prepared from disodium adenosine triphosphate after being stored in a brown bottle at 5°C under dry conditions for one month;

[0136] Sample filtrate 2: A 1% aqueous solution of disodium adenosine triphosphate prepared after being stored in a brown bottle at room temperature for one month;

[0137] Sample filtrate 3: A 1% aqueous solution of adenosine triphosphate disodium prepared and stored in a brown bottle at room temperature for one week.

[0138] from Figure 1 As can be seen, storing disodium adenosine triphosphate (ATP) in a solid state under drying conditions at 5°C and room temperature can largely prevent the hydrolysis of ATP. Therefore, storing ATP under room temperature or low-temperature drying conditions (e.g., below 25°C) can effectively protect ATP. Consequently, the ATP in the anti-hair loss and hair growth composition of this invention exhibits excellent biological stability, resulting in superior activity of the composition and thus better efficacy in preventing hair loss and promoting hair growth.

[0139] Application Examples 1-7

[0140] The anti-hair loss and hair growth composition samples from Examples 1-7 were added to the scalp serum formulations in Table 1 at a mass percentage of 2%. Seven scalp serums were prepared according to the following method.

[0141] The specific preparation method is as follows: Samples for each application example are prepared according to the formulation ratio in Table 1. The preparation method is as follows: At room temperature, deionized water, glycerin, panthenol, sodium PCA, disodium EDTA, phenoxyethanol, and ethylhexylglycerin are mixed evenly according to the formulation ratio to obtain a liquid phase; a pH adjuster (citric acid or arginine) is mixed evenly with the anti-hair loss and hair growth composition to obtain a solid phase; the solid and liquid phases are separately bottled to obtain the scalp essence. When using, the solid and liquid phases are mixed evenly.

[0142] Table 1

[0143]

[0144] Application Comparative Examples 1-4

[0145] The anti-hair loss and hair growth composition samples of Comparative Examples 1-4 were added to the scalp serum formulas in Table 2 at a weight percentage of 2%, with the formula without the anti-hair loss and hair growth composition serving as the control group. Five scalp serums were prepared according to the following method.

[0146] Prepare the comparative samples according to the formulation ratios in Table 2. The preparation method is as follows: At room temperature, mix deionized water, glycerin, panthenol, sodium PCA, disodium EDTA, phenoxyethanol, and ethylhexylglycerin in the formulation ratios to obtain a liquid phase; mix the pH adjuster (citric acid or arginine) with the anti-hair loss and hair growth composition to obtain a solid phase; separately fill the solid and liquid phases according to the actual usage requirements and the formulation ratios to obtain the scalp essence. When using, simply mix the solid and liquid phases thoroughly. The control group sample is obtained by mixing all raw materials at room temperature according to the formulation ratios.

[0147] Table 2

[0148] Deionized water Add to 100 Add to 100 Add to 100 Add to 100 Add to 100 glycerin 3 3 3 3 3 Panthenol 0.3 0.3 0.3 0.3 0.3 Sodium PCA 0.5 0.5 0.5 0.5 0.5 Disodium EDTA 0.05 0.05 0.05 0.05 0.05 Phenoxyethanol 0.45 0.45 0.45 0.45 0.45 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 Arginine 0.67 0 0.4 0 0 Citric acid 0 0.03 0 0.03 0.03 Comparative Example 1 Composition 2 Comparative Example 2 Composition 2 Comparative Example 3 Composition 2 Comparative Example 4 Composition 2

[0149] The serum prepared in Comparative Example 4 contained insoluble solids after solid-liquid mixing. This is because adenosine in Comparative Example 4 is poorly soluble in water. Compared to adenosine, other compositions containing disodium adenosine triphosphate have good water solubility, making product application more convenient.

[0150] Hair loss prevention and hair regrowth effect test

[0151] One hundred and ten volunteers aged 25-55 with mild to moderate hair loss were randomly divided into 11 groups of 10 each. Each group used a scalp serum prepared according to Examples 1-7, Comparative Examples 1-3, and the control group (Comparative Example 4 was not completely dissolved in the serum and was not tested for efficacy). Participants used the test sample twice daily, morning and evening, for four consecutive months. During the test, participants were prohibited from using cosmetics, medications, or devices with hair loss prevention or hair regrowth effects, and from perming or dyeing their hair except for cutting it. The hair loss prevention and hair regrowth effects of the samples were evaluated after 1, 2, 3, and 4 months of use.

[0152] Judgment criteria

[0153] Subjects rated the effectiveness of the hair loss prevention and hair regrowth program on a scale of 0-5, with higher scores indicating more significant effects.

[0154] 0 points: No obvious effect on preventing hair loss and promoting hair growth;

[0155] 1-3 points: The number of hair loss cases is significantly reduced, but the amount of hair in the tested area does not increase significantly;

[0156] 4-5 points: The amount of hair loss is significantly reduced, and the amount of hair in the tested area is significantly increased.

[0157] 0 points were recorded as invalid; 1-3 points as valid; and 4-5 points as effective. The statistical results are shown in Table 3.

[0158] Table 3

[0159]

[0160] As shown in Table 3, in the first month, except for a few subjects in Application Examples 2-5 who showed anti-hair loss effects, the other application examples, comparative examples, and control group did not show significant anti-hair loss and hair regrowth effects. This may be related to the hair growth cycle. The composition involved in this invention mainly acts on hair follicles, and in the three cycles of hair follicle development, the anagen phase generally lasts 2-7 years, the catagen phase is about 2-4 weeks, and the telogen phase is about 2-3 months. Therefore, one month is insufficient for hair follicles in the telogen phase to grow new hair. In the second to fourth months, more and more subjects in all application examples and comparative examples began to report anti-hair loss effects, and the sum of effective and significantly effective subjects in Application Examples 2-5 reached 80% or more after 4 months, indicating that the anti-hair loss and hair regrowth composition involved in this invention has excellent anti-hair loss and hair regrowth effects (e.g., ...). Figure 2 (As shown).

[0161] Two participants in the control group reported anti-hair loss effects after 4 months, possibly because the moisturizers in the basic scalp serum also helped repair the scalp and hair follicles.

[0162] Comparing Application Comparative Example 1 and Application Comparative Example 2 in Table 3, it can be found that the hair loss prevention and hair growth effect of hydrolyzed soy protein or disodium adenosine triphosphate alone is not as good as the effect of the two raw materials combined, indicating that there is a certain synergistic effect between the two. Moreover, the test results of Application Examples 1-7 show that the hair growth effect of Application Examples 2-5 is better than that of other application examples, indicating that this synergistic effect requires the two raw materials to be in a certain proportion to achieve better results.

[0163] In Table 3, Comparative Example 3 uses a combination of commercially available hydrolyzed soy protein and disodium adenosine triphosphate (DPT). Its effect on preventing hair loss and promoting hair growth is far inferior to that of the hydrolyzed soy protein and DPT prepared in this invention (Application Example 3). The reason may be that the molecular weight of commercially available hydrolyzed soy protein is too large, making it difficult to penetrate and thus affecting its efficacy.

[0164] It should be noted that although the technical solution of the present invention has been described with specific examples, those skilled in the art will understand that the present invention should not be limited thereto.

[0165] The various embodiments of the present invention have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or technical improvements to the embodiments in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A non- shedding hair growth composition characterized in that, It includes hydrolyzed soy protein and disodium adenosine triphosphate; wherein, based on the total mass of the anti-hair loss and hair growth composition as 100%, the content of the hydrolyzed soy protein is 10-90%, and the content of the disodium adenosine triphosphate is 10-90%. The mass ratio of the hydrolyzed soy protein to the disodium adenosine triphosphate is 1:(0.25~4). The method for preparing hydrolyzed soybean protein includes the following steps: Mix soy protein and water evenly to obtain a premix; After adjusting the pH of the premix, a protease was added to the premix to carry out a hydrolysis reaction, and the hydrolysis product was obtained. After freeze-drying the hydrolysate, hydrolyzed soybean protein is obtained; The protease is an alkaline protease; Based on the total mass of the soybean protein being 100%, the amount of the protease used is 1-2.5%; The pH of the premix is ​​adjusted to 8.0-10.5; The hydrolysis reaction takes 3-6 hours and is carried out at a temperature of 50-65°C.

2. The anti-alopecia composition according to claim 1, characterized by The mass ratio of the hydrolyzed soy protein to the disodium adenosine triphosphate is 1:(0.4~2.5).

3. The anti-alopecia composition according to claim 1, wherein Before freeze-drying, the process includes ultrasonic treatment of the hydrolysate, followed by enzyme inactivation and sterilization, solid-liquid separation, and collection of the supernatant.

4. The anti-alopecia composition according to claim 3, characterized by The ultrasonic treatment time is 10-40 minutes.

5. A process for the preparation of the anti-hair loss composition according to any one of claims 1-4, characterized in that, The step includes mixing the components of the hair loss prevention and hair growth composition.

6. Use of the anti-hair loss and hair growth composition according to any one of claims 1-4 in the preparation of scalp care products or hair cleaning products.

7. Use according to claim 6, characterized in that, The scalp care products include one or more of scalp serums, conditioners, and hair masks; the hair cleaning products include shampoos.

8. A scalp serum, characterized in that, Includes the hair loss prevention and hair growth composition according to any one of claims 1-4.

9. The scalp serum of claim 8, wherein, Based on the total mass of the scalp essence as 100%, the content of the anti-hair loss and hair growth composition is 0.01-5%.

10. The scalp serum according to claim 8 or 9, characterized in that, The scalp serum also includes one or more of the following: moisturizers, preservatives, chelating agents, pH adjusters, and solvents.

11. The scalp serum of claim 10, wherein, Based on the total mass of the scalp essence as 100%, the content of the moisturizer is 3-10%, the content of the preservative is 0.1-1%, the content of the chelating agent is 0.02-0.1%, the content of the pH adjuster is 0-2%, and the content of the solvent is 81.9-96.87%.

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