A miticide composition and its application

Abstract

The present invention belongs to the technical field of pesticide acaricides, and discloses an acaricidal composition and its application. The active ingredients in the acaricidal composition include active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is any one of fluazinam or pyridaben. The acaricidal composition of the present invention has a significant synergistic effect on controlling phytophagous mites such as Panonychus citri, effectively reduces the dosage of pesticides, and is safe for the environment.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticidal acaricides, and discloses an acaricidal composition and its application. Background Art

[0002] Fluazinam, with a CAS registration number of 79622-59-6, and a chemical name of 3-chloro-N-[3-chloro-2,6-dinitro-4-(trifluoromethyl)phenyl]-5-(trifluoromethyl)pyridin-2-amine. It is an oxidative phosphorylation uncoupler, which has excellent control effects on plant diseases such as early blight and late blight, and also has a certain control effect on harmful mites.

[0003] Pyrimidifen, with a CAS registration number of 1257598-43-8, and a chemical name of methyl (E)-2-[2-[[2-(2,4-dichloroanilino)-6-(trifluoromethyl)pyrimidin-4-yl]oxymethyl]phenyl]-3-methoxyprop-2-enoate, belongs to a mitochondrial respiratory chain inhibitor.

[0004] Phytophagous mites are closely related to agricultural production, and have characteristics such as a large number of species, wide distribution, and polyphagy. They are recognized as a pest population that is relatively difficult to control, and their main group is the Tetranychidae family. Phytophagous mites suck the sap on the front or back of plant leaves as adult, nymph, and larval mites, which enhances the transpiration of leaf surface moisture, reduces chlorophyll, inhibits photosynthesis, thereby causing the leaves to change color and wither, and in severe cases, leading to the death of the entire plant.

[0005] For a long time, agricultural production has mainly relied on chemical control to control large-scale outbreaks of mite damage. The special ecological strategies and environmental adaptability of harmful mites have made the problem of their drug resistance more and more prominent. Moreover, the extensive use of pesticides has also aggravated environmental pollution and damaged the natural ecological balance. Therefore, the applicant compounded sulfiflumin with any one of fluazinam or pyrimidifen, and found that it has a significant synergistic effect on the control of phytophagous mites, slows down the development of mite drug resistance, and is environmentally safe. Summary of the Invention

[0006] Based on the above content, the purpose of the present invention is to provide an acaricidal composition. The acaricidal composition of the present invention has a significant synergistic effect on controlling phytophagous mites such as Panonychus citri, effectively reduces the dosage of pesticides, and is environmentally safe.

[0007] Another purpose of the present invention is to provide an acaricidal composition that is safe for crops;

[0008] To achieve the above object, the present invention adopts the following technical solution. A miticidal composition, wherein the active ingredients of the miticidal composition comprise active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is any one of fluazinam or pyridaben. The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 42:1.

[0009] Further, the active ingredient B in the miticidal composition is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 42:1, such as 1:30, 1:24, 1:12, 1:6, 1:3, 6:1, 10:1, 24:1, 35:1, 42:1 or any value between the above values;

[0010] Even further, the active ingredient B in the miticidal composition is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 35:1, such as 1:24, 1:12, 1:6, 1:3, 6:1, 10:1, 24:1, 35:1 or any value between the above values;

[0011] Further, the active ingredient B in the miticidal composition is pyridaben, and the mass ratio of the active ingredient A to the active ingredient B is 1:28 to 25:1, such as 1:28, 1:15, 2:9, 2:3, 3:2, 6:1, 12:1, 25:1 or any value between the above values;

[0012] Even further, the active ingredient B in the miticidal composition is pyridaben, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 12:1, such as 1:15, 2:9, 2:3, 3:2, 6:1, 12:1 or any value between the above values;

[0013] Further, in addition to the active ingredients, the miticidal composition further comprises agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, preservatives, stabilizers, synergists, fillers, solvents or carriers;

[0014] Further, the wetting agent is one or more of alkyl benzene sulfonates, alkyl naphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, Chinese honey locust powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0015] Further, the dispersant is one or more of lignosulfonate, alkylnaphthalenesulfonate formaldehyde condensate, naphthalenesulfonate, triphenylvinylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0016] Further, the emulsifier is one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0017] Further, the thickener is one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica white; and / or

[0018] Further, the disintegrant is one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0019] Further, the antifreeze is one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0020] Further, the defoamer is one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0021] Further, the preservative is one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0022] Further, the stabilizer is one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica white, talc powder, montmorillonite, and starch; and / or

[0023] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0024] Further, the filler is a solvent, a filler, or a carrier;

[0025] Further, the solvent is one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivative, and water; and / or

[0026] Further, the carrier is one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil, and vegetable oil derivative.

[0027] Further, the formulation form of the acaricidal composition is solution, suspension, emulsifiable concentrate, emulsion in water, microcapsule suspension, microemulsion, suspo-emulsion, dispersible oil suspension, granule, water dispersible granule, wettable powder;

[0028] Even further, the formulation form is suspension, emulsifiable concentrate, water dispersible granule, wettable powder.

[0029] The present invention also discloses the application of the acaricidal composition as described above in controlling phytophagous mites.

[0030] Further, the phytophagous mites are mites of the family Tetranychidae.

[0031] Even further, the mites of the family Tetranychidae are Panonychus citri, Panonychus ulmi, Tetranychus cinnabarinus, or Tetranychus urticae.

[0032] Further, the acaricidal composition is applied to mites and the plants on which they grow or the environment in which they grow.

[0033] The beneficial effects of the present invention are as follows:

[0034] 1) The acaricidal composition of the present invention has an obvious synergistic effect in controlling phytophagous mites in a certain proportion, especially has a good control effect on Panonychus citri;

[0035] 2) The acaricidal composition of the present invention can significantly reduce the mite population, reduce the damage of mites to crops, improve the quick-acting property of the medicament, and delay the development of drug resistance;

[0036] 3) The acaricidal composition of the present invention is safe and efficient, and is safe for crops, non-target organisms, beneficial organisms, and natural enemies. Detailed Embodiments

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the preparation examples and specific examples of the present invention's formulations. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0038] Preparation Example 1: 28% Sulfiflumin·fluazinam water dispersible granule (1:6)

[0039] Formulation composition: 4% Sulfiflumin, 24% fluazinam, 10% sodium salt of naphthalene sulfonic acid formaldehyde polymer, 2% sodium polycarboxylate, 4% Nekal BX, kaolin to make up the balance;

[0040] Preparation method: According to the formulation ratio, add the active ingredient to the carrier, add surfactants and other functional aids thereto, mix, add 10 - 25% water after airflow comminution, and then obtain the water dispersible granule product through kneading, granulation, drying and screening; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0041] Preparation Example 2: 18% Sulfiflumin·fluazinam suspension concentrate (8:1)

[0042] Formulation composition: 16% Sulfiflumin, 2% fluazinam, 2% alkylphenol polyoxyethylene ether, 1% sodium polycarboxylate, 4% phosphate ester salt of alkylphenol polyoxyethylene ether, 2% alkylaryl polyoxyethylene polyoxypropylene ether, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 4% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0043] Preparation method: According to the formulation ratio, place the active ingredient, surfactants and other functional aids in the reaction kettle in sequence, add water and mix evenly, carry out high-speed shearing and wet grinding, and finally homogenize and filter to obtain the suspension concentrate product.

[0044] Preparation Example 3: 22% Sulfiflumin·pyridaben suspension concentrate (2:9)

[0045] Formulation composition: 4% Sulfiflumin, 18% pyridaben, 2% polyoxyethylene glycerol fatty acid ester, 2% alkylaryl polyoxyethylene polyoxypropylene ether, 1% naphthalene sulfonate formaldehyde condensate, 5% polyoxyethylene sorbitan monooleate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.4% silicone oil, deionized water to make up the balance;

[0046] Preparation method: According to the formulation ratio, place the active ingredient, surfactants and other functional aids in the reaction kettle in sequence, add water and mix evenly, carry out high-speed shearing and wet grinding, and finally homogenize and filter to obtain the suspension concentrate product.

[0047] Preparation Example 4: 21% Sulfiflumin·pyridaben water dispersible granule (6:1)

[0048] Formulation composition: 18% sulfiflumin, 3% pyridaben, 8% dispersant MF-5, 2% sodium polycarboxylate, 2% sodium dodecyl sulfate, and light calcium carbonate to make up the balance.

[0049] Preparation method: According to the formulation ratio, add the active ingredients to the carrier, and add surfactants and other functional auxiliaries thereto, mix, add 10-25% water after air-flow pulverization, and then obtain the water-dispersible granule product through kneading, granulation, drying, and screening; or spray water, granulate, and dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0050] Preparation Example 5: 15% sulfiflumin·pyridaben wettable powder (2:3)

[0051] Formulation composition: 6% sulfiflumin, 9% pyridaben, 10% calcium lignosulfonate, 2% dispersant NNO, 2% sodium dodecyl sulfate, and kaolin to make up the balance;

[0052] Preparation method: According to the formulation ratio, mix the active ingredients, dispersants, wetting agents, and fillers, stir evenly in a stirring kettle, and perform multiple pulverizations and mixings evenly with an air-flow pulverizer to obtain the wettable powder of the composition of the present invention.

[0053] Example 1: Indoor bioassay activity determination

[0054] Test basis: The test refers to NY / T1154.13-2008 "Pesticide indoor bioassay test guidelines Insecticides Part 13: Leaf disc spraying method".

[0055] Biological test materials: Panonychus citri.

[0056] Test agents: sulfiflumin technical, fluazinam technical, pyridaben technical.

[0057] Test preparation: Select citrus leaves with consistent growth and not treated with any pesticides, make leaf discs with a hole puncher, place a wet sponge block in a petri dish, place filter paper on it, place leaf discs on the filter paper, 2 leaf discs per dish, inoculate test mites onto the leaf discs, 15 mites per leaf disc.

[0058] Agent preparation: Prepare the technical with an organic solvent to make a mother liquor, and then prepare 5 series of mass concentrations according to the equal ratio method with 0.1% Tween 80 aqueous solution.

[0059] Agent treatment: Place the petri dish on the bottom plate of the Potter spray tower for spraying, the spraying liquid amount is 1 mL, take it out after the agent has settled for 1 min, and transfer it to be raised under normal conditions. Each treatment has 4 replicates, and a treatment without agent (containing all organic solvents and emulsifiers) is set as a blank control.

[0060] Rearing and Observation: The treated mite specimens were reared and observed under the conditions of temperature (25±1)°C and photoperiod L:D=(16:8)h.

[0061] Inspection: The mortality of the test insects was inspected 48 h after treatment, and the total number of mites and the number of dead mites were recorded respectively.

[0062] Calculation Method:

[0063] Based on the investigation data, the mortality of each treatment was calculated. It was calculated according to the following formula:

[0064]

[0065] In the formula:

[0066] P —— Mortality, in percentage (%);

[0067] K —— Number of dead insects, in number;

[0068] N —— Total number of treated insects, in number.

[0069]

[0070] In the formula:

[0071] P1 —— Corrected mortality, in percentage (%);

[0072] P t —— Mortality of treatment, in percentage (%);

[0073] P0 —— Mortality of blank control, in percentage (%).

[0074] If the control mortality < 5%, no correction is needed; if the control mortality is between 5% and 20%, correction should be made according to formula (2); if the control mortality > 20%, the test needs to be redone.

[0075] The co-toxicity coefficient (CTC value) of the mixture was calculated according to formulas (3), (4), and (5):

[0076]

[0077] In the formula:

[0078] ATI —— Measured toxicity index of the mixture;

[0079] S —— LC of the standard acaricide 50 , in milligrams per liter (mg / L);

[0080] M —— LC of the mixture 50, in milligrams per liter (mg / L).

[0081] TTI = TI A × P A + TI B × P B

[0082] Where:

[0083] TTI——Theoretical toxicity index of the mixture;

[0084] TI A ——Toxicity index of chemical A;

[0085] P A ——Percentage content of chemical A in the mixture, in percentage (%);

[0086] TI B ——Toxicity index of chemical B;

[0087] P B ——Percentage content of chemical B in the mixture, in percentage (%).

[0088]

[0089] Where:

[0090] CTC——Coefficient of co-toxicity;

[0091] ATI——Actual toxicity index of the mixture;

[0092] TTI——Theoretical toxicity index of the mixture.

[0093] When the co-toxicity coefficient CTC of the compound is ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect. The results of the indoor toxicity test are shown in the following table:

[0094] Table 1 Results of the indoor bioactivity determination test of sulfiflumin and fluazinam against Panonychus citri

[0095] Test agents Toxicity regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Sulfiflumin(A) y = 4.3996 + 1.1979x 0.9864 3.1714 / Fluazinam(B) y = 3.3178 + 1.2794x 0.9952 20.6438 / A:B = 1:30 y = 3.5771 + 1.2794x 0.9919 12.9476 135.381 A:B = 1:24 y = 3.6477 + 1.3110x 0.9966 10.7529 157.315 A:B = 1:12 y = 3.6724 + 1.4325x 0.9968 8.4477 171.634 A:B = 1:6 y = 4.0083 + 1.2429x 0.9941 6.2789 183.979 A:B = 1:3 y = 4.1927 + 1.2235x 0.9954 4.5691 190.050 A:B = 6:1 y = 4.6808 + 1.3639x 0.9943 1.7141 210.466 A:B = 10:1 y = 4.6223 + 1.3663x 0.9897 1.8899 181.796 A:B = 24:1 y = 4.6108 + 1.2646x 0.9980 2.0312 161.605 A:B = 35:1 y = 4.6044 + 1.1026x 0.9941 2.2847 142.152 A:B = 42:1 y = 4.5167 + 1.1738x 0.9811 2.5807 125.357 A:B = 55:1 y = 4.4469 + 1.1477x 0.9915 3.0336 106.147

[0096] Table 2 Results of the indoor bioactivity determination test of sulfiflumin and pyridaben against Panonychus citri

[0097]

[0098]

[0099] As can be seen from the indoor test results in Table 1-2, the acaricidal composition of the present invention has a good control effect on Panonychus citri. When sulfiflumin is compounded with fluazinam, within the mass ratio range of 1:30 to 42:1, it shows a synergistic effect on Panonychus citri; when the mass ratio is 1:24 to 35:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is obvious; when the mass ratio is 1:12 to 10:1, the co-toxicity coefficient is greater than 170, and the synergistic effect is significant; while when the mass ratio is 55:1, it shows an additive effect.

[0100] When sulfiflumin is compounded with pyridaben, the mass ratio of the two is 1:28 to 25:1, showing a synergistic effect; when the mass ratio is 1:15 to 12:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is obvious; when the mass ratio is 1:15 to 6:1, the co-toxicity coefficient is greater than 140, and the synergistic effect is significant; while when the mass ratio is 1:42, 30:1 to 40:1, it shows an additive effect.

[0101] Example 2: Field efficacy control test against citrus red mites

[0102] Test site: Citrus orchard in Huangyan District, Taizhou City, Zhejiang Province. The test crop is Satsuma mandarin. The citrus red mites have been seriously harmful in the test site all year round. All test plots are uniform and consistent, meeting local scientific agricultural practices.

[0103] Test plot setting: A total of 5 medicament treatments and 1 clear water control treatment are set in the test. Each plot is arranged in a random block design. The area of each treatment plot is 2 citrus trees, and each treatment is repeated 4 times.

[0104] Pesticide application time and method: During pesticide application, the conventional crown spraying method is adopted, and a knapsack manual sprayer is used to apply pesticides to citrus trees. The pesticide application was carried out on May 15, 2023. The weather was normal during the test, and there were no adverse climate factors affecting the test results. During pesticide application, the test medicaments were diluted with water according to the designed dosage, and the application was carried out evenly and thoroughly until the leaves were dripping with water.

[0105] Investigation time and method: The pest population base was investigated before pesticide application, and the number of surviving mites was investigated 1 time each at 3 days, 15 days, and 30 days after pesticide application, for a total of 4 investigations. In each plot, 1 tree tip was marked at the east, south, west, north, and middle of each tree, and the 5 topmost leaves of each tree tip were investigated. A total of 50 leaves on each plot were investigated for the number of active mites, and the number of live mites was counted.

[0106] Efficacy calculation method:

[0107]

[0108]

[0109] Field efficacy test results and analysis:

[0110]

[0111]

[0112] The investigation results 3 days after application showed that the control effects of the compound acaricidal composition of the present invention against Panonychus citri were 87.87% and 87.50%, both higher than those of each control single agent; 15 days after application, the control effects of the four agents of 18% sulfiflumin·fluazinam suspension concentrate (8:1) and 15% sulfiflumin·pyridaben wettable powder (2:3) were 93.46% and 92.60% respectively; 30 days after application, the control effects of the acaricidal composition of the present invention against Panonychus citri were significantly higher than those of each single agent control, and the long-lasting period was longer. Within the tested concentration range, no obvious phytotoxicity of the acaricidal composition of the present invention to citrus leaves, young shoots and fruits was observed after application.

[0113] Although the present invention has been described in detail with general descriptions and specific embodiments in the above text, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A miticidal composition, characterized in that, The active ingredients of the acaricidal composition comprise active ingredient A and active ingredient B. The active ingredient A is sulfiflumin, and the active ingredient B is fluazinam. The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 42:

1.

2. The acaricidal composition according to claim 1, wherein The mass ratio of the active ingredient A to the active ingredient B in the acaricidal composition is 1:24 to 35:

1.

3. The acaricidal composition according to claim 1, wherein In addition to the active ingredients, the acaricidal composition further comprises agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are fillers and / or surfactants.

4. The acaricidal composition according to claim 1, wherein The preparation dosage form of the acaricidal composition is a solution, suspension, emulsifiable concentrate, emulsion in water, microcapsule suspension, microemulsion, suspo-emulsion, dispersible oil suspension, granule, water dispersible granule or wettable powder.

5. The acaricidal composition according to claim 4, characterized in that, The preparation dosage form is a suspension, emulsifiable concentrate, water dispersible granule or wettable powder.

6. Application of the acaricidal composition according to any one of claims 1-5 in controlling Panonychus citri.

7. The application according to claim 6, wherein Apply the acaricidal composition to Panonychus citri and the plants on which it grows or its growth environment.

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