Floor sludge microbial treatment inducer and its application

Abstract

The application provides a floor oil sludge microbial treatment inducer and application thereof. The floor oil sludge microbial treatment inducer comprises a modified pretreatment agent and an exogenous microbial enzyme activity promoter; the modified pretreatment agent comprises sodium carboxymethyl cellulose, sodium carboxyethylidene diphosphonate, sodium humate, kaolinite and ethanol; and the exogenous microbial enzyme activity promoter comprises a KH2PO4-K2HPO4 buffer, yeast powder, lactose, linseed oil and petroleum ether. The floor oil sludge microbial treatment inducer can solve the problem of low efficiency of microbial treatment of floor oil sludge soil in the prior art and is suitable for the field of oil pollution treatment.

Description

Technical Field

[0001] This invention relates to the field of petroleum pollution treatment, and more specifically, to a microbial inducing agent for the treatment of landed oil sludge and its application. Background Technology

[0002] Oil pollution is a major environmental problem. Bioremediation, first proposed in the 1980s, refers to engineering technologies that utilize the metabolic activities of organisms to degrade hazardous pollutants in the environment into water and carbon dioxide or transform them into other non-toxic or low-toxic substances. Bioremediation is a novel method for soil pollution control. It boasts advantages such as minimizing pollutant concentrations, maintaining or improving soil structure, minimal environmental impact without secondary pollution, simple operation, and low treatment costs. In recent years, it has received widespread attention both domestically and internationally and is considered one of the most promising soil purification technologies.

[0003] However, microbial remediation technology also has certain limitations. For example, excessively high pollutant concentrations, poor performance of oily sludge or petroleum-contaminated soil, the presence of harmful components in the degradation environment, insufficient nutrients and mineral elements to stimulate catalytic enzyme activity, and the use of a single type of degrading bacteria can all affect or inhibit the microbial degradation effect. In addition, the long time required for microbial degradation is also a factor that limits the widespread application of this technology. Therefore, although microbial degradation technology has obvious advantages, due to its limitations, it still needs to be further studied and optimized to improve the treatment effect in terms of environmental protection and large-scale industrial application. The content of this patent is based on research and experimental application conducted in this context. Summary of the Invention

[0004] The main objective of this invention is to provide a microbial inducing agent for treating landed oil sludge and its application, so as to solve the problem of low efficiency in the treatment of landed oil sludge soil by microorganisms in the prior art.

[0005] To achieve the above objectives, according to a first aspect of the present invention, a microbial treatment inducer for landed oil sludge is provided, the microbial treatment inducer for landed oil sludge comprising: a modified pretreatment agent and an exogenous microbial enzyme activity promoter; the modified pretreatment agent comprising: sodium carboxymethyl cellulose, sodium carboxyethylidene diphosphonate, sodium humate, kaolinite and ethanol; the exogenous microbial enzyme activity promoter comprising: KH2PO4-K2HPO4 buffer, yeast powder, lactose, linseed oil and petroleum ether.

[0006] Furthermore, the modified pretreatment agent uses 0.1-0.5 wt% sodium carboxymethyl cellulose as the base liquid; the modified pretreatment agent also includes 0.001-0.01 wt% sodium dithiocarbamate, 0.05-0.1 wt% sodium carboxyethylidene diphosphonate, 0.5-2 wt% sodium humate, 0.5-2 wt% kaolinite, and 0.1%-1 wt% ethanol.

[0007] Furthermore, the exogenous microbial enzyme activity promoter uses a 1 mol / L KH2PO4-K2HPO4 buffer as the base solution, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the exogenous microbial enzyme activity promoter also includes 10-25 wt% yeast extract, 0.5-1.5 wt% lactose, 2-5 wt% linseed oil, and 0.5-2 wt% petroleum ether.

[0008] Furthermore, the inducing agent for treating sludge microorganisms also includes a native microbial activator, which comprises: KH2PO4-K2HPO4 buffer, sodium nitrite, magnesium sulfate, ferrous sulfate, dodecyl dimethyl betaine, and hydroxylase; preferably, the hydroxylase includes ribulose diphosphate carboxylase; preferably, the native microbial activator uses 1 mol / L KH2PO4-K2HPO4 buffer as the base solution, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the native microbial activator also includes 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, and 2-8 wt% hydroxylase; preferably, the preparation temperature and storage temperature of the native microbial activator are both 20-35℃.

[0009] To achieve the above objectives, according to a second aspect of the present invention, an application of the above-mentioned inducing agent for the treatment of landed oil sludge is provided, the application comprising: a) applying a modified pretreatment agent to the landed oil sludge soil to modify the landed oil sludge soil and reduce its environmental toxicity; b) applying an exogenous microbial enzyme activity promoter and exogenous microorganisms to the landed oil sludge soil to induce and enhance the activity of the exogenous microorganisms.

[0010] Further, a) includes: crushing the landed oily mud soil and adding a modified pretreatment agent; preferably, the particle size of the crushed landed oily mud soil is 0.1cm-1cm; preferably, the landed oily mud soil is tilled after adding the modified pretreatment agent, and the treatment time of the modified pretreatment agent is 12-24h.

[0011] Furthermore, the amount of modified pretreatment agent used, based on the amount of sodium dithiocarbamate, is such that the mass ratio of sodium dithiocarbamate to metal ions in the landed oil sludge is 0.0005-0.001:1; preferably, the metal ions include Fe.3+ Cu 2+ Zn 2+ Pb 2+ Mn 2+ Cr 6+ or Cr 3+ One or more of them.

[0012] Further, the mass ratio of the exogenous microbial enzyme activity promoter to the landed oil sludge soil is 0.01-0.05:1, and the mass ratio of the exogenous microorganisms to the oil content in the landed oil sludge soil is 0.01-0.02:1; preferably, the exogenous microorganisms include one or more of Bacillus pumilus, Pseudomonas aeruginosa, or Acinetobacter loufi; preferably, the exogenous microorganisms include microbial inoculants, and the viable count in the microbial inoculants is greater than 10. 9 per ml.

[0013] Furthermore, the treatment temperature of the exogenous microbial enzyme activity promoter is 10-35℃; preferably, the treatment humidity of the exogenous microbial enzyme activity promoter is 45%-75%; preferably, the treatment is stopped when the oil content of the landed oily mud soil is <0.9%.

[0014] Furthermore, between a) and b), the method further includes: c) applying a native microbial activator to the landed oil sludge soil to induce an increase in the number and activity of native microorganisms in the landed oil sludge soil; the native microbial activator includes: KH2PO4-K2HPO4 buffer, sodium nitrite, magnesium sulfate, ferrous sulfate, dodecyl dimethyl betaine, and hydroxylase; preferably, the hydroxylase includes ribulose diphosphate carboxylase; preferably, the native microbial activator uses 1 mol / L KH2PO4-K2HPO4 buffer as the base solution, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the native microbial activator further includes 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, 2-8 wt% hydroxylase; preferably, the amount of local microbial activator is based on the amount of hydroxylase, and the mass ratio of hydroxylase to landed oil sludge is 0.0001-0.0005:1; preferably, the treatment temperature of the local microbial activator is 10-35℃; preferably, the treatment time of the local microbial activator is 7-9 days.

[0015] Applying the technical solution of the present invention, the above-mentioned microbial treatment inducer for landed oil sludge includes a modified pretreatment agent and an exogenous microbial enzyme activity promoter. The above components can improve the composition of the landed oil sludge soil and enhance the ability and efficiency of microorganisms to degrade petroleum hydrocarbons. Detailed Implementation

[0016] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the embodiments.

[0017] Terminology Explanation:

[0018] Oil sludge: A mixture of oil and mud sand generated during oil and gas exploration, extraction, gathering and transportation, and wastewater (liquid) treatment, as well as oil-bearing rock cuttings generated during drilling using oil-based mud.

[0019] Oily sludge soil: Soil contaminated by the aforementioned oily sludge.

[0020] As mentioned in the background section, microbial remediation technology is a relatively good method for soil pollution control among existing technologies, but it still has certain limitations, as the degradation activity and efficiency of microorganisms are easily affected. Therefore, in this application, the inventors attempted to develop a microbial inducing agent for treating landed oil sludge that can improve the efficiency of microbial treatment of landed oil sludge, and thus proposed a series of protection schemes in this application.

[0021] In a first typical embodiment of this application, a microbial treatment inducer for landed oil sludge is provided. The microbial treatment inducer for landed oil sludge includes: a modified pretreatment agent and an exogenous microbial enzyme activity promoter; the modified pretreatment agent includes: sodium carboxymethyl cellulose, sodium carboxyethylidene diphosphonate, sodium humate, kaolinite, and ethanol; the exogenous microbial enzyme activity promoter includes: KH2PO4-K2HPO4 buffer, yeast powder, lactose, linseed oil, and petroleum ether.

[0022] The aforementioned modified pretreatment agents contain organic particles and organic matter (such as kaolinite) capable of adsorbing metal ions, complexing agents and shielding agents (such as sodium carboxyethylidene diphosphonate, which has both complexing and shielding functions) capable of forming stable compounds with harmful metal ions, and dispersants (such as sodium carboxymethyl cellulose) capable of improving the aggregation state of petroleum hydrocarbons in landfill sludge. These modified pretreatment agents can modify landfill sludge soil, reducing its environmental toxicity, especially the toxicity of harmful metal ions to microorganisms.

[0023] The aforementioned exogenous microbial enzyme activity promoters, based on the degradation mechanism of exogenous microorganisms, can promote microbial proliferation by optimizing carbon sources and culture media. Activators that activate enzymes can form a ternary complex of enzyme-metal ion-substrate between the enzyme and substrate, which facilitates the binding of the substrate to the active site of the enzyme and enhances the degradation capacity of petroleum in landfill sludge.

[0024] In a preferred embodiment, the modified pretreatment agent uses 0.1-0.5 wt% sodium carboxymethyl cellulose as the base liquid; the modified pretreatment agent also includes 0.001-0.01 wt% sodium dithiocarbamate, 0.05-0.1 wt% sodium carboxyethylidene diphosphonate, 0.5-2 wt% sodium humate, 0.5-2 wt% kaolinite, and 0.1-1 wt% ethanol.

[0025] In a preferred embodiment, the exogenous microbial enzyme activity promoter uses a 1 mol / L KH2PO4-K2HPO4 buffer solution as the base solution, and the pH of the KH2PO4-K2HPO4 buffer solution is 7.0-7.5; the exogenous microbial enzyme activity promoter also includes 10-25 wt% yeast extract, 0.5-1.5 wt% lactose, 2-5 wt% linseed oil, and 0.5-2 wt% petroleum ether.

[0026] In a preferred embodiment, the inducing agent for treating sludge microorganisms further includes a native microbial activator, which comprises: KH2PO4-K2HPO4 buffer, sodium nitrite, magnesium sulfate, ferrous sulfate, dodecyl dimethyl betaine, and hydroxylase; preferably, the hydroxylase comprises ribulose diphosphate carboxylase; preferably, the native microbial activator uses a 1 mol / L KH2PO4-K2HPO4 buffer as the base solution, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the native microbial activator further comprises 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, and 2-8 wt% hydroxylase; preferably, the preparation temperature and storage temperature of the native microbial activator are both 20-35℃.

[0027] Hydroxylases, also known as oxygenases or dehydrogenases, including but not limited to ribulose diphosphate hydroxylase, can catalyze oxidation in the process of microbial degradation of petroleum hydrocarbons, significantly increasing the rate of oxidative degradation of hydrocarbons.

[0028] The aforementioned native microbial activator, based on the native microbial community in the oil sludge soil, enhances the proliferation and activity of native microorganisms. Among these native microorganisms are numerous native strains capable of tolerating and degrading petroleum. The nutrients in the activator create an environment suitable for the reproduction of native microorganisms. The enzymes in the activator promote the degradation of petroleum by microorganisms. While enhancing the degradation capacity, the proliferation of native microorganisms also improves the soil environment, thus facilitating the subsequent proliferation and activity of exogenous microorganisms. Because the activator contains enzymes, its preparation and storage temperatures are preferably controlled between 20 and 35°C to maximize its activity.

[0029] In a second typical embodiment of this application, an application of the above-mentioned microbial treatment inducer for landed oil sludge is provided, the application including: a) applying a modified pretreatment agent to the landed oil sludge soil to modify the landed oil sludge soil and reduce environmental toxicity; b) applying an exogenous microbial enzyme activity promoter and exogenous microorganisms to the landed oil sludge soil to induce and enhance the activity of exogenous microorganisms.

[0030] In the above application, the landed oil sludge soil is first modified using a pretreatment agent to adsorb, complex, and shield heavy metals in the soil, and disperse aggregated petroleum hydrocarbons and other organic matter. Through physical and chemical means, the soil is improved, reducing its toxicity and creating a suitable environment for microbial growth and reproduction. After modification with the pretreatment agent, exogenous microorganisms and exogenous microbial enzyme activity promoters are added to the landed oil sludge soil. Under the action of the exogenous microbial enzyme activity promoters, the exogenous microorganisms in the modified soil achieve a faster proliferation rate and a faster degradation rate of petroleum hydrocarbons.

[0031] In a preferred embodiment, a) includes: crushing the fallen oily mud soil and adding a modified pretreatment agent; preferably, the particle size of the crushed fallen oily mud soil is 0.1cm-1cm; preferably, the fallen oily mud soil is tilled after adding the modified pretreatment agent, and the treatment time of the modified pretreatment agent is 12-24h.

[0032] By breaking up and tilling the soil, the modified pretreatment agent can be thoroughly mixed with the landed oily sludge, increasing the contact area and thus improving the modification effect of the pretreatment agent. The treatment time for the modified pretreatment agent after tilling is 12-24 hours. After this treatment time, the modified pretreatment agent is considered to have fully exerted its effect and subsequent treatments can proceed.

[0033] In a preferred embodiment, the amount of modified pretreatment agent used is based on the amount of sodium dithiocarbamate, and the mass ratio of sodium dithiocarbamate to metal ions in the landed oil sludge is 0.0005-0.001:1; preferably, the metal ions include Fe. 3+ Cu 2+ Zn 2+ Pb 2+ Mn 2+ Cr 6+ or Cr 3+ One or more of them.

[0034] In the use of modified pretreatment agents, the total mass concentration of the aforementioned metal ions in the soil is first determined. The mass of sodium dithiocarbamate in the modified pretreatment agent to be used is controlled to be 2%-10% of the total mass of metal ions in the soil. This addition amount ensures complete treatment of metal ions in the soil. It also disperses petroleum hydrocarbons in the soil, preventing problems such as poor aeration caused by soil adhesion, and providing a more suitable environment for subsequent microbial growth. Furthermore, it avoids the problem of additional pollution caused by excessive reagent addition.

[0035] In a preferred embodiment, the mass ratio of the exogenous microbial enzyme activity promoter to the exogenous microorganism is 0.01-0.05:1, including 0.03:1, and the mass ratio of the exogenous microorganism to the oil content (i.e., petroleum hydrocarbons) in the landed oil sludge is 0.01-0.02:1; preferably, the exogenous microorganism includes, but is not limited to, one or more of Bacillus pumilus, Pseudomonas aeruginosa, or Acinetobacter rofibrinosus; preferably, the exogenous microorganism includes a microbial inoculum, wherein the microbial inoculum has a viable count greater than 10. 9 per ml.

[0036] Microbial inoculants are industrial products obtained from the above-mentioned exogenous microorganisms through three-stage fermentation. They are products prepared using existing microbial inoculant preparation processes and can be either liquid or solid. When using them, the mass ratio of the microbial inoculant to the oil content in the fallen oil sludge is 0.01~0.02:1, and the viable count in the microbial inoculant is greater than 10. 9 per ml.

[0037] In section b), after treatment with a modified pretreatment agent, exogenous microbial enzyme activity promoters and exogenous microorganisms are applied to the landed oil sludge soil. The exogenous microorganisms, capable of degrading petroleum hydrocarbons, are used to treat the landed oil sludge soil. The mass ratio of exogenous microorganisms to petroleum hydrocarbons in the landed oil sludge soil is 0.01–0.02:1, and the mass ratio of exogenous microbial enzyme activity promoters to exogenous microorganisms is 0.03:1. That is, the amount of exogenous microbial enzyme activity promoter added is 0.03%–0.06% of the mass of petroleum hydrocarbons in the landed oil sludge soil.

[0038] The aforementioned exogenous microorganisms include strains of bacteria capable of degrading petroleum hydrocarbons in the prior art, including but not limited to one or more of Bacillus pumilus, Pseudomonas aeruginosa, or Acinetobacter rhusiopathiae. Exogenous microorganisms can be used in various forms of microbial agents, including but not limited to liquids, powders, or granules prepared using existing technologies.

[0039] In a preferred embodiment, the treatment temperature of the exogenous microbial enzyme activity promoter is 10-35°C; preferably, the treatment humidity of the exogenous microbial enzyme activity promoter is 45%-75%; preferably, the treatment is stopped when the oil content of the landed oily sludge is <0.9%. The oil content is the mass percentage of petroleum hydrocarbons in the landed oily sludge.

[0040] The above-mentioned exogenous microbial enzyme activity promoter was used to treat the landed oil sludge soil at a temperature of 10-35℃ and a humidity of 45%-75%. Under these temperature and humidity conditions, the exogenous microorganisms exhibited a high proliferation rate and a high rate of metabolism of petroleum hydrocarbons, thus reducing the petroleum hydrocarbon content in the landed oil sludge soil more quickly. Treatment was considered complete when the oil content in the landed oil sludge soil was <0.9%. The oil content is the percentage of the mass of petroleum hydrocarbons in the landed oil sludge soil relative to the total mass of the soil.

[0041] In a preferred embodiment, between a) and b), the method further includes: c) applying a native microbial activator to the landed oil sludge soil to induce an increase in the number and activity of native microorganisms in the landed oil sludge soil; the native microbial activator includes: KH2PO4-K2HPO4 buffer, sodium nitrite, magnesium sulfate, ferrous sulfate, dodecyl dimethyl betaine, and hydroxylase; preferably, the hydroxylase includes ribulose diphosphate carboxylase; preferably, the native microbial activator uses 1 mol / L KH2PO4-K2HPO4 buffer as the base solution, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the native microbial activator further includes 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, 2-8 wt% hydroxylase; preferably, the amount of local microbial activator is based on the amount of hydroxylase, and the mass ratio of hydroxylase to landed oil sludge is 0.0001-0.0005:1; preferably, the treatment temperature of the local microbial activator is 10-35℃; preferably, the treatment time of the local microbial activator is 7-9 days.

[0042] After modification with a pretreatment agent and before applying exogenous microbial enzyme activity promoters and exogenous microorganisms, native microbial activators can be used to induce the proliferation of native microorganisms in the landed oil sludge soil. Native microorganisms are the original strains found in the landed oil sludge soil. Native microorganisms that can survive in landed oil sludge soil are tolerant of this environment, and many strains have evolved the ability to metabolize petroleum hydrocarbons. Therefore, inducing the proliferation of native microorganisms can not only restore soil biodiversity and improve soil conditions, but also degrade petroleum hydrocarbons in the soil. The dosage of the native microbial activator is based on the amount of hydroxylase; after application, the hydroxylase accounts for 0.01%-0.05% of the mass of the landed oil sludge soil. Because the process involves the release of enzyme activity and the proliferation of native microorganisms, the treatment temperature of the native microbial activator is 10-35℃ to allow the hydroxylase to exert its activity better, enabling the native microorganisms to proliferate and metabolize petroleum hydrocarbons more effectively. Due to significant environmental variations during implementation, such as the oil content in the deposited oily sludge, ambient temperature, and humidity, the total treatment time will be affected. Treatment time is shorter in summer than in winter. Using the above method, the total treatment time is approximately 50-60 days in summer, 60-90 days in spring and autumn, and 90-120 days in winter. The specific time will fluctuate depending on actual environmental changes.

[0043] The beneficial effects of this application will be explained in more detail below with reference to specific embodiments.

[0044] Example 1

[0045] 1. Calculate the weight of the oil sludge soil to be treated, 100 tons.

[0046] 2. Crush the soil to be treated to a particle size of less than 1 cm, mix it evenly, and test the oil content to be 5%.

[0047] 3. Detection of metal ions (Fe) in the soil to be treated. 3+ Cu 2+ Zn 2+ Pb 2+ Mn 2+ Cr 6+ and Cr 3+ The total amount is 400 mg / kg, which means the metal ion content is 40 kg.

[0048] 4. On June 1st, add the pre-modified oil sludge pretreatment agent. Prepare the pretreatment agent by adding 4 kg of sodium carboxymethyl cellulose (i.e., preparing a base liquid of 0.2 wt% sodium carboxymethyl cellulose) to 2 tons of deionized water, adding 2 kg of sodium carboxyethylidene diphosphonate (0.1 wt%), 20 kg of sodium humate (1%), 40 kg of kaolinite (2 wt%), 10 kg of ethanol (0.5 wt%), and 40 g of sodium dithiocarbamate (0.1 wt% of the total metal ion content, i.e., 0.02 wt% of the pretreatment agent). After adding the above agents to the base liquid, stir thoroughly. The pretreatment agent is now ready. Add the pretreatment agent to the oil sludge soil to be treated as prepared in step 3. Control the humidity at 55%-65%.

[0049] 5. Apply native microbial activators. Prepare 2 tons of 1 mol / L KH₂PO₄-K₂HPO₄ buffer solution (pH 7.0-7.5), add 20 kg of 1 wt% sodium nitrite, 40 kg of 2 wt% magnesium sulfate, 40 kg of 2 wt% ferrous sulfate, and 3 kg of 0.3 wt% dodecyl dimethyl betaine. Add hydroxylase at a concentration of 0.08% of the weight of the soil to be treated (80 kg, i.e., 4 wt% of the native microbial activator). After adding the reagents according to the dosage, mix thoroughly. Add to the soil to be treated, till evenly, and treat for 7 days, controlling humidity at 45%-75% and temperature at 10℃-35℃.

[0050] 6. Add microbial inoculant to the oily sludge soil after step 5, at a rate of 5% of the oil content, i.e., add 2.5 tons of microbial inoculant. After adding, till / mix evenly.

[0051] 7. Apply exogenous microbial enzyme activity promoters. Prepare 2 tons of 1 mol / L KH2PO4-K2HPO4 buffer (pH 7.0-7.5), add 15 wt% yeast powder (300 kg), 1 wt% lactose (20 kg), 3 wt% linseed oil (60 kg), and 1 wt% petroleum ether (20 kg), stir well, and then add to the contaminated oil sludge soil treated in step 6.

[0052] 8. After step 7 is completed, the oil content should be tested weekly, and the change in oil content should be recorded to determine the total processing time. The processing is considered complete when the oil content is less than 0.9%. The experimental data are shown in Table 1.

[0053] Example 2

[0054] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0055] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0056] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0057] Example 3

[0058] The operation was simultaneously carried out on 100 tons of oily sludge soil with an oil content of 10%, the operation being the same as in Example 1, except that:

[0059] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0060] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0061] Example 4

[0062] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0063] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0064] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0065] Example 5

[0066] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0067] The modified pretreatment agent and the exogenous microbial enzyme activity promoter have different compositions, and the relevant steps of applying the local microbial activator were not performed.

[0068] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0069] Comparative Example 1

[0070] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0071] The components of the exogenous microbial enzyme activity promoters differed, and the steps of applying modified pretreatment agents and native microbial activators were not performed.

[0072] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0073] Comparative Example 2

[0074] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0075] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0076] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0077] Comparative Example 3

[0078] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0079] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0080] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0081] Comparative Example 4

[0082] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0083] The components of modified pretreatment agents, native microbial activators, and exogenous microbial enzyme activity promoters differ.

[0084] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0085] Comparative Example 5

[0086] The operation was simultaneously carried out on the same landed oily mud soil with the same soil conditions as in Example 1, except that:

[0087] The modified pretreatment agent and the native microbial activator have different compositions, and the relevant steps of applying exogenous microbial enzyme activity promoters were not performed.

[0088] Oil content was measured weekly, and the change in oil content was recorded as the total processing time. Processing was considered complete when the oil content was less than 0.9%. The experimental data are shown in Table 1.

[0089] Table 1

[0090]

[0091] Note: "-" indicates that the corresponding ingredient was not added, and "\" indicates that this step was not performed.

[0092] As can be seen from the above description, the above-described embodiments of the present invention achieve the following technical effects: the above-described inducing agent for treating oily sludge can modify the soil of oily sludge to reduce environmental toxicity and enhance the activity of exogenous microorganisms in degrading petroleum hydrocarbons; furthermore, it can also activate local microorganisms, and restore the microbial diversity in the soil while degrading petroleum hydrocarbon pollution.

[0093] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A floor sludge microbial treatment inducer, characterized by, The landing oil sludge microbial treatment inducer comprises a modified pretreatment agent and an exogenous microbial enzyme activity promoter. The modified pretreatment agent takes 0.1-0.5 wt% sodium carboxymethyl cellulose as a base solution, and further comprises 0.001-0.01 wt% sodium dithiocarbamate, 0.05-0.1 wt% sodium carboxyethylidene diphosphonate, 0.5-2 wt% sodium humate, 0.5-2 wt% kaolinite, and 0.1%-1 wt% ethanol. The exogenous microbial enzyme activity promoter takes 1 mol / L KH2PO4-K2HPO4 buffer solution as a base solution, the pH of the KH2PO4-K2HPO4 buffer solution is 7.0-7.5, and the exogenous microbial enzyme activity promoter further comprises 10-25 wt% yeast powder, 0.5-1.5 wt% lactose, 2-5 wt% linseed oil, and 0.5-2 wt% petroleum ether. The landing oil sludge microbial treatment inducer further comprises an indigenous microbial activator. The indigenous microbial activator takes 1 mol / L KH2PO4-K2HPO4 buffer solution as a base solution, the pH of the KH2PO4-K2HPO4 buffer solution is 7.0-7.5, and the indigenous microbial activator further comprises 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, and 2-8 wt% hydroxylase.

2. The floor sludge microbial treatment inducer according to claim 1, characterized in that, The hydroxylase comprises ribulose bisphosphate carboxylase.

3. The floor sludge microbial treatment inducer according to claim 1, characterized in that, The configuration temperature and the storage temperature of the indigenous microbial activator are both 20-35℃.

4. Use of a floor sludge microbial treatment inducer as claimed in any one of claims 1 to 3, characterized in that, The application comprises: a) applying the modified pretreatment agent to the landing oil sludge soil to modify the landing oil sludge soil and reduce environmental toxicity; b) applying the exogenous microbial enzyme activity promoter and exogenous microorganisms to the landing oil sludge soil to induce the activity of the exogenous microorganisms.

5. Use according to claim 4, characterized in that, The a) comprises adding the modified pretreatment agent after crushing the landing oil sludge soil.

6. Use according to claim 5, characterized in that, The particle size of the landing oil sludge soil after crushing is 0.1 cm-1 cm.

7. Use according to claim 5, characterized in that, The landing oil sludge soil is plowed after adding the modified pretreatment agent, and the treatment time of the modified pretreatment agent is 12-24 h.

8. Use according to claim 4, characterized in that, The amount of the modified pretreatment agent is calculated based on the amount of sodium dithiocarbamate, and the mass ratio of sodium dithiocarbamate to metal ions in the landing oil sludge soil is 0.0005-0.001:

1.

9. Use according to claim 8, characterized in that, The metal ions include one or more of Fe 3+ , Cu 2+ , Zn 2+ , Pb 2+ , Mn 2 + , Cr 6+ or Cr 3+ .

10. Use according to claim 4, characterized in that, The mass ratio of the exogenous microbial enzyme activity promoter to the landing oil sludge soil is 0.01-0.05:1, and the mass ratio of the exogenous microorganisms to the oil content in the landing oil sludge soil is 0.01-0.02:

1.

11. Use according to claim 4, characterized in that, The exogenous microorganisms comprise one or more of Bacillus pumilus, Pseudomonas aeruginosa, or Acinetobacter lwoffii.

12. Use according to claim 4, characterized in that, The exogenous microorganism includes a microbial inoculant having a viable count greater than 10 9 cells / ml.

13. Use according to claim 4, characterized in that, The treatment temperature of the exogenous microbial enzyme activity promoter is 10-35℃.

14. The use according to claim 4, characterized in that, The treatment humidity of the exogenous microbial enzyme activity promoter is 45%-75%.

15. The use according to claim 4, characterized in that, When the oil content of the landing oil sludge soil is <0.9%, the treatment is stopped.

16. The use according to claim 4, characterized in that, Between the a) and the b), further comprising: c) applying a native microbe activator to the oil sludge soil to induce increase of the number and activity of native microbes in the oil sludge soil; The native microbe activator comprises: KH2PO4-K2HPO4 buffer, sodium nitrite, magnesium sulfate, ferrous sulfate, dodecyl dimethyl betaine and hydroxylase.

17. Use according to claim 16, characterized in that, The hydroxylase comprises ribulose bisphosphate carboxylase.

18. The use according to claim 16, characterized in that, The native microbe activator takes 1 mol / L KH2PO4-K2HPO4 buffer as base fluid, and the pH of the KH2PO4-K2HPO4 buffer is 7.0-7.5; the native microbe activator further comprises 0.5-3 wt% sodium nitrite, 1-5 wt% magnesium sulfate, 1-5 wt% ferrous sulfate, 0.1-1 wt% dodecyl dimethyl betaine, and 2-8 wt% hydroxylase.

19. The use according to claim 16, characterized in that, The amount of the native microbe activator is 0.0001-0.0005:1 of the mass ratio of the hydroxylase to the oil sludge soil.

20. The use according to claim 16, characterized in that, The treatment temperature of the native microbe activator is 10-35℃.

21. The use according to claim 16, characterized in that, The treatment time of the native microbe activator is 7-9 days.

Images