A premix for pig feed, a preparation method and application thereof

Abstract

The application relates to the feed technology field, and discloses a premix agent for pig feed, a preparation method and application thereof.The premix agent for pig feed comprises the following raw materials in parts by weight: 12-25 parts of Clostridium butyricum fermentation liquor, 5-15 parts of Artemisia princeps extract and 8-15 parts of Astragalus henryi; wherein the viable cell count of Clostridium butyricum in the Clostridium butyricum fermentation liquor is 3.8-4.2*10 9 cfu / g.The premix agent for pig feed prepared by the application is a high-efficiency and environment-friendly feed additive, which can effectively improve the health level, production performance and breeding benefit of piglets.

Description

TECHNICAL FIELD

[0001] The application relates to the feed technology field, in particular to a premix agent for pig feed, a preparation method and application thereof. BACKGROUND

[0002] Piglets are the core of modern pig production, and their health status has an extremely important influence on the growth and development in the later period. The biggest problem faced by large-scale breeding is that piglets are prone to diseases, and the key to the health of a pig herd lies in enhancing the disease resistance of piglets.

[0003] After weaning, piglets change from sucking milk to eating solid feed, the digestive tract and digestive glands are not yet fully developed, and the secretion of digestive enzymes is insufficient, which leads to the fact that piglets do not have the ability to digest a large amount of plant feed. At the same time, piglets have electrolyte imbalance and acid-base balance disorder in the body. In addition, the maternal antibodies obtained by newborn piglets from colostrum decrease over time, and the protein, immunoglobulin and other substances in colostrum decrease to the lowest level at the third week after delivery, while the immune system of piglets has not yet been fully established, which leads to poor disease resistance of piglets. In addition, piglets will experience stress in spirit and physiology after leaving the sow, and the piglets are not adapted to the new environment, which leads to the fact that piglets have conditional diseases, which will disturb the immunity and growth performance of piglets, and further lead to the emaciation and death of piglets.

[0004] Therefore, there is an urgent need to provide a premix agent for feed that can improve the immunity of piglets and promote the growth of piglets. SUMMARY

[0005] In order to solve the above at least one technical problem, a premix agent for feed that can improve the immunity of piglets and promote the growth of piglets is developed, and the application provides a premix agent for pig feed, a preparation method and application thereof.

[0006] In one aspect, the application provides a premix agent for pig feed, which comprises the following raw materials in parts by weight: Clostridium butyricum fermentation broth 12-25 parts, artemisia princeps extract 5-15 parts, and astragalus sinicus 8-15 parts.

[0007] The number of viable Clostridium butyricum in the Clostridium butyricum fermentation broth is 3.8-4.2*10 9 cfu / g.

[0008] By adopting the above technical scheme, the premix agent for pig feed prepared by the application is a high-efficiency and environmentally-friendly feed additive, which can effectively improve the health level, production performance and breeding benefit of piglets.

[0009] The Clostridium butyricum fermentation broth used in the present application contains a large amount of Clostridium butyricum and rich metabolic products, and various digestive enzymes, amino acids and vitamins and other substances beneficial to animal health produced can help animals better digest and absorb the nutrients in the feed, thereby improving the growth performance and immunity of animals; and can regulate intestinal microorganisms and their metabolites, inhibit the reproduction of harmful bacteria and promote the reproduction of beneficial bacteria to maintain intestinal health and improve intestinal barrier function, and the improvement of intestinal barrier function can effectively reduce the absorption of harmful substances in the intestine, thereby helping to maintain the immune function of piglets; and can promote the proliferation of epithelial cells and the secretion of antibacterial peptides, as well as intercellular tight junction proteins, secreted immunoglobulin A and the expression of regulatory cytokines, thereby enhancing the barrier function and immunity of the piglets' intestines, and also helping to promote the healing and tissue repair of the piglets' intestines;

[0010] The Clostridium butyricum fermentation broth used in the present application contains a large amount of Clostridium butyricum and rich metabolic products, and various digestive enzymes, amino acids and vitamins and other substances beneficial to animal health produced can help animals better digest and absorb the nutrients in the feed, thereby improving the growth performance and immunity of animals; and can regulate intestinal microorganisms and their metabolites, inhibit the reproduction of harmful bacteria and promote the reproduction of beneficial bacteria to maintain intestinal health and improve intestinal barrier function, and the improvement of intestinal barrier function can effectively reduce the absorption of harmful substances in the intestine, thereby helping to maintain the immune function of piglets; and can promote the proliferation of epithelial cells and the secretion of antibacterial peptides, as well as intercellular tight junction proteins, secreted immunoglobulin A and the expression of regulatory cytokines, thereby enhancing the barrier function and immunity of the piglets' intestines, and also helping to promote the healing and tissue repair of the piglets' intestines;

[0011] The Clostridium butyricum fermentation broth used in the present application contains a large amount of Clostridium butyricum and rich metabolic products, and various digestive enzymes, amino acids and vitamins and other substances beneficial to animal health produced can help animals better digest and absorb the nutrients in the feed, thereby improving the growth performance and immunity of animals; and can regulate intestinal microorganisms and their metabolites, inhibit the reproduction of harmful bacteria and promote the reproduction of beneficial bacteria to maintain intestinal health and improve intestinal barrier function, and the improvement of intestinal barrier function can effectively reduce the absorption of harmful substances in the intestine, thereby helping to maintain the immune function of piglets; and can promote the proliferation of epithelial cells and the secretion of antibacterial peptides, as well as intercellular tight junction proteins, secreted immunoglobulin A and the expression of regulatory cytokines, thereby enhancing the barrier function and immunity of the piglets' intestines, and also helping to promote the healing and tissue repair of the piglets' intestines;

[0012] Optionally, the Clostridium butyricum fermentation broth is 18-20 parts, the Artemisia rupestris L. extract is 8-12 parts, and the Astragalus henryi subsp. is 10-12 parts.

[0013] By adopting the above technical solution, the present application selects a more optimal range of Clostridium butyricum fermentation broth, Artemisia rupestris L. extract and Astragalus henryi subsp. to prepare a premix agent for pig feed, which has more excellent comprehensive performance, better immunity and growth performance.

[0014] Optionally, the mesh number of the Artemisia rupestris L. extract is 80-120.

[0015] By adopting the above technical solution, the present application selects a more optimal range of Clostridium butyricum fermentation broth, Artemisia rupestris L. extract and Astragalus henryi subsp. to prepare a premix agent for pig feed, which has more excellent comprehensive performance, better immunity and growth performance.

[0016] Optionally, the mesh number of the Astragalus henryi subsp. is 80-120.

[0017] By adopting the technical scheme, the more optimal mesh number of the astragalus henryi seeds is selected, so that the astragalus henryi seeds are uniformly dispersed in the feed, and the loose property and the mixing uniformity of the feed are ensured; and the nutritional components such as protein, minerals and vitamins are better released, and the nutritional value of the feed is improved.

[0018] Optionally, the difference between the mesh number of the astragalus henryi seeds and the mesh number of the artemisia princeps extract is not greater than 30 mesh.

[0019] By adopting the technical scheme, the difference between the mesh number of the astragalus henryi seeds and the mesh number of the artemisia princeps extract is controlled, so that the difference between the mesh numbers is not too large, which can avoid the uneven distribution of the astragalus henryi seeds and the artemisia princeps extract in the feed, and further avoid the uneven release and absorption in the feed, and affect the growth and immune function of the pig.

[0020] Optionally, the bee wax is further included, and the weight of the bee wax is 0.5-1 part.

[0021] By adopting the technical scheme, the bee wax is further added, so that the stability, the nutritional value and the dispersing property of the prepared pig feed premix are improved; the bee wax added in the pig feed premix has a porous structure, uniformly wraps the pig feed premix as a carrier, improves the antioxidant property and the stability of the pig feed premix, and can increase the consistency and the viscosity of the pig feed premix, so that the pig feed premix is more uniformly dispersed in the feed, the loose property and the mixing uniformity of the feed are improved, the pig feed premix is prevented from being agglomerated in the feed, and the comprehensive performance of the pig feed premix is improved.

[0022] In a second aspect, the application provides a preparation method of a pig feed premix, including the following steps:

[0023] S1, the artemisia princeps extract and the astragalus henryi seeds are respectively subjected to drying pretreatment to prepare pretreated artemisia princeps extract and pretreated astragalus henryi seeds;

[0024] S2, the pretreated artemisia princeps extract and the pretreated astragalus henryi seeds are subjected to mixing and stirring treatment to prepare a premix;

[0025] S3, the clostridium butyricum fermentation liquor is mixed and stirred with the premix to obtain the pig feed premix.

[0026] By adopting the technical scheme, the preparation method of the pig feed premix is simple in operation, the raw materials are natural and environmentally friendly, and the pig feed premix can be produced industrially; the pig feed premix can promote the growth and immune function of the pig, and can also improve the intestinal health of the pig.

[0027] Optionally, the preparation method of the clostridium butyricum fermentation liquor in S3 includes the following steps:

[0028] The Clostridium butyricum is inoculated into the fermentation medium at a volume fraction of 5-10% to prepare the Clostridium butyricum fermentation liquor through fermentation culture.

[0029] Optionally, the fermentation culture temperature is 35-40 DEG C, and the fermentation culture time is 15-20h.

[0030] By adopting the technical scheme, the Clostridium butyricum fermentation liquor prepared by the application is rich in Clostridium butyricum and various metabolic products of Clostridium butyricum, such as organic acids like butyric acid, lactic acid and acetic acid, and various enzyme substances, which can positively affect the intestinal health and immunity of pigs.

[0031] In a third aspect, the application provides an application of a premix agent for pig feed, which is used for preparing piglet feed, and the weight of the premix agent for pig feed is 2.3-5.6wt% of the weight of the piglet feed.

[0032] In summary, the application has at least one of the following beneficial technical effects:

[0033] 1. The premix agent for pig feed prepared by the application is a high-efficiency and environment-friendly feed additive, which can effectively improve the health level, production performance and breeding benefit of piglets.

[0034] 2. The Clostridium butyricum fermentation liquor used by the application contains a large amount of Clostridium butyricum and rich metabolic products, and various digestive enzymes, amino acids and vitamins and other beneficial animal health substances can help animals better digest and absorb the nutritional ingredients in feed, thereby improving the growth performance and immunity of animals.

[0035] 3. The specific amount of artemisia princeps extract used by the application contains various bioactive substances, which can promote the reproduction of intestinal microorganisms, improve the intestinal environment, improve the digestive capacity, improve the secretion of immunoglobulin, improve the immunity, regulate the immune stress response, promote the growth performance and shorten the breeding cycle.

[0036] 4. The specific amount of Astragalus sinicus used by the application is rich in protein, fat, minerals and vitamins and other nutrients, and the addition of Astragalus sinicus in pig feed can promote the growth and development of pigs, improve the immunity, and has excellent functions of anti-fatigue, anti-oxidation and anti-inflammation.

[0037] 5. The preparation method of the premix agent for pig feed is simple in operation, the raw materials are natural and environment-friendly, and the premix agent for pig feed can be produced industrially. DETAILED DESCRIPTION

[0038] The application will be further described in detail below in combination with examples.

[0039] The application designs a premix agent for pig feed, which comprises the following raw materials in parts by weight: Clostridium butyricum fermentation liquor 12-25 parts, artemisia princeps extract 5-15 parts, and astragalus sinicus 8-15 parts;

[0040] Among them, the viable count of Clostridium butyricum in the Clostridium butyricum fermentation liquor is 3.8-4.2*10 9 cfu / g.

[0041] The application also provides a preparation method of the premix agent for pig feed, which comprises the following steps:

[0042] S1, taking artemisia princeps extract and astragalus sinicus respectively for drying pretreatment to prepare pretreated artemisia princeps extract and pretreated astragalus sinicus;

[0043] S2, mixing and stirring the pretreated artemisia princeps extract and astragalus sinicus to prepare a premix;

[0044] S3, taking the Clostridium butyricum fermentation liquor and the premix for stirring and mixing treatment to obtain the premix agent for pig feed.

[0045] The application also provides an application of the premix agent for pig feed, which is used for preparing piglet feed, and the weight of the premix agent for pig feed is 2.3-5.6 wt% of the weight of the piglet feed.

[0046] The application provides the following raw material models, and if no special instructions are given, the raw materials in the application are all from the market:

[0047] Artemisia princeps extract: select artemisia princeps extract with a specification of 20:1 from Xi'an Guohao Biological Technology Co., Ltd., and grind the artemisia princeps extract to obtain the required 80-120 mesh;

[0048] Astragalus sinicus: select astragalus sinicus with a purity of 99% from Shuyang County Baifuyuan Landscape Co., Ltd., and grind the astragalus sinicus to obtain the required 80-120 mesh;

[0049] Clostridium butyricum: CAS number 9012-54-8;

[0050] Bee wax: CAS number 8012-89-3;

[0051] Glucose: CAS number 50-99-7;

[0052] Isobutene: CAS number 115-11-7;

[0053] Sodium glutamate: CAS number 142-47-2;

[0054] Pine needle powder: Fufeng Snoot Biological Technology Co., Ltd., specification 10:1;

[0055] Kelp powder: CAS number, 9012-54-8;

[0056] Calcium carbonate: CAS number, 471-34-1;

[0057] Manganese sulfate: CAS number, 7785-87-7;

[0058] Piglet feed: Yiyang Shenghai Feed Technology Co., Ltd., 851 piglet feed.

[0059] Detection items:

[0060] Experimental design: 40-day-old piglets were selected, 20 heads as a group, and the test period was 40 days. Among them, the pig feed prepared by the premixing agent of the application was mixed and stirred with pig feed to feed piglets; after the test, the average daily weight gain (kg), average daily feed intake (kg), feed conversion ratio and IgG, IgA and IgM contents of piglets were detected. Specific embodiments

[0062] Examples 1-5

[0063] The specific amount of raw materials of a pig feed premixing agent is shown in Table 1.

[0064] Table 1

[0065]

[0066] Among them, the mesh number of artemisia princeps extract is 80 mesh, and the mesh number of astragalus sonso is 120 mesh.

[0067] Example 1

[0068] A preparation method of a pig feed premixing agent, comprising the following steps:

[0069] S1, taking artemisia princeps extract and astragalus sonso respectively for drying pretreatment, to prepare pretreated artemisia princeps extract and pretreated astragalus sonso;

[0070] S2, mixing and stirring the pretreated artemisia princeps extract and astragalus sonso to prepare a premixing material;

[0071] S3, taking butyric acid clostridium fermentation broth and premixing material for stirring and mixing treatment to obtain a pig feed premixing agent;

[0072] The preparation method of butyric acid clostridium fermentation broth in S3 comprises the following steps: butyric acid clostridium is inoculated in fermentation medium at a volume fraction of 5%, cultured at 35℃ for 15h, and fermented and cultured to prepare butyric acid clostridium fermentation broth, and the viable count of butyric acid clostridium in the prepared butyric acid clostridium fermentation broth is 3.8*10 9 cfu / g;

[0073] The raw materials of the fermentation medium are glucose, isobutene, sodium glutamate, pine needle powder, kelp powder, calcium carbonate, manganese sulfate and water, and the weight ratio is 1:0.5:0.02:0.1:0.1:2:0.1:95.

[0074] Example 2

[0075] A preparation method of a premix agent for pig feed comprises the following steps:

[0076] S1, taking Artemisia rupestris L. extract and Astragalus henryi extracts respectively for drying pretreatment to prepare pretreated Artemisia rupestris L. extract and pretreated Astragalus henryi extract;

[0077] S2, mixing and stirring the pretreated Artemisia rupestris L. extract and Astragalus henryi extract to prepare a premix;

[0078] S3, taking Clostridium butyricum fermentation broth and the premix for stirring and mixing treatment to obtain a premix agent for pig feed;

[0079] The preparation method of the Clostridium butyricum fermentation broth in S3 comprises the following steps: inoculating Clostridium butyricum into a fermentation medium at a volume fraction of 7%, culturing at 38°C for 18h for fermentation culture, and the viable bacterial count of Clostridium butyricum in the prepared Clostridium butyricum fermentation broth is 4.0*10 9 cfu / g;

[0080] The raw materials of the fermentation medium are glucose, isobutene, sodium glutamate, pine needle powder, kelp powder, calcium carbonate, manganese sulfate and water, and the weight ratio is 1:0.5:0.02:0.1:0.1:2:0.1:95.

[0081] Example 3

[0082] A preparation method of a premix agent for pig feed comprises the following steps:

[0083] S1, taking Artemisia rupestris L. extract and Astragalus henryi extracts respectively for drying pretreatment to prepare pretreated Artemisia rupestris L. extract and pretreated Astragalus henryi extract;

[0084] S2, mixing and stirring the pretreated Artemisia rupestris L. extract and Astragalus henryi extract to prepare a premix;

[0085] S3, taking Clostridium butyricum fermentation broth and the premix for stirring and mixing treatment to obtain a premix agent for pig feed;

[0086] The preparation method of the Clostridium butyricum fermentation broth in S3 comprises the following steps: inoculating Clostridium butyricum into a fermentation medium at a volume fraction of 10%, culturing at 40°C for 20h for fermentation culture, and the viable bacterial count of Clostridium butyricum in the prepared Clostridium butyricum fermentation broth is 4.2*10 9 cfu / g;

[0087] The raw materials of the fermentation medium are glucose, isobutene, sodium glutamate, pine needle powder, kelp powder, calcium carbonate, manganese sulfate and water, and the weight ratio is 1:0.5:0.02:0.1:0.1:2:0.1:95.

[0088] Example 4-5

[0089] Based on Example 2, except that the raw materials of the premix for pig feed are different, the rest of the preparation method is consistent with Example 2.

[0090] Comparative Example 1

[0091] Based on Example 2, except that an equal amount of Clostridium butyricum is replaced by Clostridium butyricum fermentation broth, the rest of the components and preparation method are consistent with Example 2.

[0092] Comparative Example 2

[0093] Based on Example 2, except that an equal amount of Astragalus subshrub is replaced by Artemisia princeps extract, the rest of the components and preparation method are consistent with Example 2.

[0094] Comparative Example 3

[0095] Based on Example 2, except that an equal amount of Artemisia princeps extract is replaced by Astragalus subshrub, the rest of the components and preparation method are consistent with Example 2.

[0096] The pig feed premix prepared in Examples 1-5 and Comparative Examples 1-3 is used to feed piglets (wherein the weight of the pig feed premix of Example 1 is 2.3wt% of the weight of the pig feed, the weight of the pig feed premix of Example 2 is 4.2wt% of the weight of the pig feed, the weight of the pig feed premix of Example 3 is 5.6wt% of the weight of the pig feed, and the weight of the pig feed premix of Examples 4-5 and Comparative Examples 1-3 is 4.2wt% of the weight of the pig feed), and the indicators of the piglets after feeding are detected, and the results are shown in Table 2.

[0097] Table 2

[0098]

[0099] As can be seen from Examples 1-3 and Table 2, the pig feed premix prepared in the present application can effectively improve the health level, production performance and breeding benefit of piglets, the average daily gain is at least 0.6kg, the feed-meat ratio is at most 1.71, and the immune capacity is improved, wherein the immunoglobulin A is at least 1.1g / L, the immunoglobulin G is at least 8.67g / L, and the immunoglobulin M is at least 0.93g / L;

[0100] As can be seen from Examples 2, 4-5 and Table 2, the pig feed premix prepared by selecting a more optimal range of Clostridium butyricum fermentation liquor, artemisia japonica extract and astragalus extract has more excellent comprehensive performance, better immunity and growth performance;

[0101] As can be seen from Comparative Example 2, Example 2 and Table 2, the pig feed premix prepared by selecting an equal amount of Clostridium butyricum to replace Clostridium butyricum fermentation liquor has poor comprehensive performance, low growth performance and low immunity, which affects the breeding benefit. The inventor speculates that the pig feed premix prepared by using Clostridium butyricum fermentation liquor contains rich metabolic products, and various digestive enzymes, amino acids and vitamins and other substances beneficial to animal health can help animals better digest and absorb the nutrients in the feed, thereby improving the growth performance and immunity of animals;

[0102] As can be seen from Comparative Examples 2-3, Example 2 and Table 2, the pig feed premix prepared by selecting artemisia japonica extract and astragalus extract has more excellent comprehensive performance.

[0103] Examples 6-8

[0104] Based on Example 4, except that the number of artemisia japonica extract and astragalus extract is different, the other components and preparation methods are the same as those of Example 4.

[0105] Example 6

[0106] The number of artemisia japonica extract is 120 mesh, and the number of astragalus extract is 110 mesh.

[0107] Example 7

[0108] The number of artemisia japonica extract is 100 mesh, and the number of astragalus extract is 100 mesh.

[0109] Example 8

[0110] The number of artemisia japonica extract is 110 mesh, and the number of astragalus extract is 80 mesh.

[0111] Examples 9-11

[0112] Based on Example 7, except that beeswax is also added, the other components and preparation methods are the same as those of Example 7.

[0113] Example 9

[0114] The weight of beeswax is 0.5 kg.

[0115] Example 10

[0116] The weight of beeswax is 0.8 kg.

[0117] Example 11

[0118] The weight of the beeswax is 1 kg.

[0119] The pig feed premix prepared in Examples 6-11 is used to feed piglets (wherein the weight of the pig feed premix of Examples 6-11 is 4.2wt% of the weight of the pig feed), and the indexes of the piglets after feeding are detected, and the results are shown in Table 3.

[0120] Table 3

[0121]

[0122]

[0123] As can be seen from Examples 4, 6-8 and Table 3, the smaller the difference between the mesh number of the Artemisia princeps extract and the mesh number of the Astragalus melilotus is, the more excellent the comprehensive performance of the prepared pig feed premix is;

[0124] As can be seen from Examples 7, 9-11 and Table 3, the pig feed premix is prepared by further adding beeswax, and it is found that the growth performance and immune ability of the piglets are improved after feeding experiment. The inventors speculate that the beeswax added in the pig feed premix has a porous structure, which uniformly wraps the pig feed premix as a carrier, improves the antioxidant property and stability of the pig feed premix, and can increase the consistency and viscosity of the premix, so that it is more uniformly dispersed in the feed, improves the bulkiness and mixing uniformity of the feed, avoids the pig feed premix from agglomerating in the feed, and improves the comprehensive performance of the pig feed premix.

[0125] The above are preferred embodiments of the present application, and do not limit the protection scope of the present application, so: any equivalent changes made according to the principles of the present application should be covered within the protection scope of the present application.

Claims

1. A premix for pig feed, characterized in that, The ingredients include the following parts by weight: Clostridium butyricum fermentation broth 12-25 parts, Artemisia argyi extract 5-15 parts, and Astragalus membranaceus seed 8-15 parts; The viable count of Clostridium butyricum in the Clostridium butyricum fermentation broth is 3.8-4.2*10⁻⁶. 9 cfu / g; The mesh size of the Artemisia argyi extract is 80-120 mesh; The mesh size of the purple clover is 80-120 mesh.

2. The premix for pig feed according to claim 1, characterized in that, The fermentation broth of Clostridium butyricum contains 18-20 parts, the extract of Artemisia capillaris contains 8-12 parts, and the seeds of Astragalus membranaceus contain 10-12 parts.

3. The premix for pig feed according to claim 1, characterized in that, The difference between the mesh size of the purple clover seed and the mesh size of the artemisia extract is no more than 30 mesh.

4. The premix for pig feed according to claim 1, characterized in that, It also includes beeswax, in the form of 0.5-1 parts by weight.

5. A method for preparing the premix for pig feed according to claim 1, characterized in that, Includes the following steps: S1. Take Artemisia scoparia extract and Astragalus membranaceus seeds and dry them separately to prepare pretreated Artemisia scoparia extract and pretreated Astragalus membranaceus seeds; S2. Mix and stir the pretreated Artemisia argyi extract and Astragalus membranaceus seeds to prepare a premix; S3. Take the Clostridium butyricum fermentation broth and mix it with the premix to obtain the premix for pig feed as described in claim 1.

6. The method for preparing the premix for pig feed according to claim 5, characterized in that, The preparation method of the Clostridium butyricum fermentation broth in S3 includes the following steps: inoculating Clostridium butyricum into the fermentation medium at a volume fraction of 5-10%, carrying out fermentation culture, and preparing Clostridium butyricum fermentation broth.

7. The method for preparing the premix for pig feed according to claim 6, characterized in that, The fermentation culture temperature is 35-40℃, and the fermentation culture time is 15-20h.

8. The application of a premix for pig feed according to any one of claims 1-4, characterized in that, The premix for pig feed is used to prepare piglet feed, and the weight of the premix for pig feed is 2.3-5.6 wt% of the weight of the piglet feed.

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