Plant foliar spray for improving drought resistance of crops

Abstract

The application discloses a kind of plant foliar spraying agent for improving crop drought resistance.The plant foliar spraying agent is the mixed solution of fatty alcohol polyoxyethylene ether and soyasaponin Bb, in the mixed solution, the ratio of the fatty alcohol polyoxyethylene ether to the soyasaponin Bb is 1 mM:2.5-5.0 mg.Through the research of the application, the growth of soybean, wheat and millet after spraying the plant foliar spraying agent of the application under water control or drought condition is better than that of the control group, and the soybean, wheat and millet are more drought-resistant, with higher proline and lower malondialdehyde content.The plant foliar spraying agent of the application has quick effect, can improve the drought resistance of plants in a short period of time, thereby improving the drought resistance of crops, thereby improving the yield of crops in seasonal drought and drought areas, achieving the purpose of stable grain yield and economic benefits.

Description

Technical Field

[0001] This invention relates to a foliar spray for improving crop drought resistance, belonging to the field of agricultural production technology. Background Technology

[0002] Drought stress is a significant factor affecting plant growth and development. In recent years, due to climate change and rising average annual temperatures, drought problems have become increasingly prominent, making the improvement of crop drought resistance a crucial issue in agricultural production. Plants have evolved a series of response mechanisms through long-term natural selection to adapt to or resist stress damage. When natural growth conditions cannot be changed, enhancing the plant's resistance to adversity can strengthen its ability to adapt to unfavorable environments. Currently, there is no rapid method to improve plant drought resistance; therefore, spraying different exogenous substance formulations to induce drought resistance in various plant varieties (especially important crop varieties) is of great significance for increasing yields under drought conditions, maintaining soil and water conservation capacity, and improving regional ecological environments. Summary of the Invention

[0003] The purpose of this invention is to provide a plant foliar spray that can improve the drought resistance of crops.

[0004] The plant foliar spray provided by this invention is a mixed solution of fatty alcohol polyoxyethylene ether and soybean saponin Bb.

[0005] The chemical formula of the fatty alcohol polyoxyethylene ether (AEO) is RO(CH2CH2O). n H, where n is a number between 6 and 8, and R represents a C12 to C18 alkyl group.

[0006] The chemical formula of the soybean saponin Bb is C. 48 H 78 O 18 Soy saponins are natural plant sterols with vital physiological functions, such as maintaining homeostasis, controlling glycogen and mineral metabolism, regulating stress responses, preventing and treating cancer, and significantly lowering blood cholesterol. Soy saponins Bb can be used as a raw material for synthesizing hormones and drugs, such as estrogen, androgens, and hydrocortisone. They can regulate human growth, reproduction, and development, and have anti-inflammatory and analgesic effects.

[0007] In the mixed solution, the ratio of the fatty alcohol polyoxyethylene ether to the soybean saponin Bb is 1 mmol: 2.5~5.0 mg, preferably 1 mmol: 2.5 mg.

[0008] Preferably, the solvent used in the mixed solution is water.

[0009] Preferably, the molar concentration of the fatty alcohol polyoxyethylene ether in the mixed solution is 0.2~0.5 mM, more preferably 0.2 mM.

[0010] The plant foliar spray can be prepared by adding an aqueous solution of the fatty alcohol polyoxyethylene ether and a methanol solution of the soybean saponin Bb to water and mixing them evenly. The plant foliar spray provided by this invention can improve the drought resistance of crops, including soybeans, wheat and millet.

[0011] Based on the research of this invention, soybeans, wheat, and millet sprayed with the foliar spray of this invention showed better growth under controlled water or drought conditions, were more drought-resistant, had higher proline content, and lower malondialdehyde content than soybeans, wheat, and millet sprayed with the control group.

[0012] The aforementioned foliar spray can be applied to seedlings during the seedling stage of crops, such as seedlings around five weeks old. Spray once daily, and every other day, between 6:00 PM and 7:00 PM, at a rate of 20-30 mL / m³. 2 Spray the above mixture.

[0013] Before applying the foliar spray of this invention, perform routine seedling management on the crops, such as: watering appropriately in the early stage of seedling growth. Keep the soil moist to ensure good seedling growth; generally, water once every 7-10 days. After the seedlings have grown for 5 weeks, implement water control measures.

[0014] Conventional methods can be used to grow crops such as soybeans, wheat, and millet, which are all planted by sowing. When sowing, seeds with high purity, few impurities, and high germination rates should be selected. After sowing, the seeds should be covered with soil immediately.

[0015] Before sowing, compound fertilizer needs to be applied to the soil. This fertilizer primarily consists of potassium sulfate, potassium nitrate, urea, and potassium dihydrogen phosphate, with a total nutrient content of no less than 45%. The N:P:K ratio is 15:6.549:12.447. This compound fertilizer has advantages such as high nutrient content, few byproducts, and good physical properties. It plays an important role in balanced fertilization, improving fertilizer utilization, and promoting high and stable crop yields. The disadvantage is that the nutrient ratio is fixed. Different soils and crops require different types, quantities, and ratios of nutrients. Therefore, it is best to conduct soil testing before use to understand the soil texture and nutrient status. Additionally, it is important to use it in conjunction with single-element fertilizers to achieve better results.

[0016] This invention provides a plant foliar spray that is fast-acting and can improve the drought resistance of plants in a short period of time, thereby improving the drought resistance of crops and increasing crop yields in seasonally and arid regions, achieving the goal of stable grain production and economic benefits. Attached Figure Description

[0017] Figure 1 This diagram illustrates the effects of different spraying treatments on the normal growth of soybeans, where the spraying formulation is a solution containing soybean saponin Bb.

[0018] Figure 2 This is a schematic diagram illustrating the effects of different spraying treatments on the drought resistance of soybeans, where the spraying formulation is a solution containing soybean saponin Bb.

[0019] Figure 3 A schematic diagram illustrating the effects of different spraying treatments on proline and malondialdehyde in soybeans under drought conditions.

[0020] Figure 4 This diagram illustrates the effects of different spraying treatments on the normal growth of wheat, where the spraying formulation is a solution containing soybean saponin Bb.

[0021] Figure 5 This is a schematic diagram illustrating the effects of different spraying treatments on wheat drought resistance, where the spraying formulation is a solution containing soybean saponin Bb.

[0022] Figure 6 A schematic diagram illustrating the effects of different spraying treatments on proline and malondialdehyde in wheat under drought conditions.

[0023] Figure 7 This diagram illustrates the effects of different spraying treatments on the normal growth of millet, where the spraying formulation is a solution containing soybean saponin Bb.

[0024] Figure 8 This diagram illustrates the effects of different spraying treatments on the drought resistance of millet, where the spraying formulation is a solution containing soybean saponin Bb.

[0025] Figure 9 A schematic diagram illustrating the effects of different spraying treatments on proline and malondialdehyde in millet under drought conditions. Detailed Implementation

[0026] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0027] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.

[0028] Example 1: Plant foliar spray The foliar sprayer provided in this embodiment is a mixed solution of AEO and soybean saponin Bb, prepared according to the following method: Add 200 μL of a 40 mM AEO aqueous solution and 50 μL of a 400 mg / L soybean saponin Bb methanol solution to 40 mL of water, mix well to obtain a plant foliar spray.

[0029] In the foliar spray provided in this embodiment, the molar concentration of AEO is 0.2 mM, the mass concentration of soybean saponin Bb is 0.5 mg / L, and the ratio of AEO to soybean saponin Bb is 1 mmol: 2.5 mg.

[0030] Example 2: Improving the drought resistance of soybeans using foliar sprays. 1. Planting of seedling crops First, place the potting soil in a square box and water it. Weigh out 10 kg of potting soil and spread it evenly in the box. Fertilize with 30 g of compound fertilizer. Pour 5 L of water into the box and mix thoroughly. Level the seedbed surface.

[0031] Soybeans are planted by sowing seeds. Sow soybean seeds 2 cm deep in the soil, spacing them 5 cm apart, with 8 seeds per row. Select seeds with high purity, few impurities, and high germination rates. Immediately after sowing, cover the seeds with soil and place them in a controlled greenhouse at 25 ℃ for growth.

[0032] The compound fertilizer used is made primarily from potassium sulfate, potassium nitrate, urea, and potassium dihydrogen phosphate, with a total nutrient content of no less than 45%. The N, P, and K ratio is 15:6.549:12.447.

[0033] 2. Seedling Management In the early stages of seedling cultivation, water appropriately. Keep the soil moist but not waterlogged to ensure healthy seedling growth. Generally, water every 7-10 days. After the seedlings have grown for 5 weeks, implement water control measures. Drought treatment: Stop watering 5-week-old seedlings and allow the soil to dry out under controlled conditions. Normal treatment: Continue watering 5-week-old seedlings, maintaining soil moisture in a controlled environment to prevent it from drying out.

[0034] 3. Spraying treatment For five-week-old seedlings growing under normal and controlled watering conditions, the foliar spray prepared in Example 1 of this invention was applied. Before spraying, seedlings treated with different methods were separated using plastic wrap to avoid cross-contamination. Spraying was performed once daily, and every other day (between 18:00 and 19:00 in the evening, at 27 mL / m²). 2 Spray the above mixture).

[0035] The control group solution was prepared by adding 200 μL of 40 mM AEO aqueous solution to 40 mL of aqueous solution.

[0036] The effects of foliar spraying on normal soybean growth, such as Figure 1 As shown in the figure, under normal conditions, the soybeans sprayed with the formulation showed better growth than the soybeans sprayed with the control group.

[0037] The effects of foliar spraying on the drought resistance of soybeans, such as Figure 2 As shown in the figure, under drought conditions, the soybeans sprayed with the formulation showed significantly better drought resistance than the soybeans sprayed with the control group.

[0038] Effects of spray formulation on proline and malondialdehyde content in soybean leaves under drought conditions, such as Figure 3 As shown, the spray formulation treatment can significantly improve the drought resistance of soybeans, increase the proline content in soybean leaves under drought conditions, and decrease the malondialdehyde content in soybean leaves under drought conditions. Example 3: Improving the drought resistance of wheat by using plant foliar sprays 1. Planting of seedling crops: Same as in Example 2.

[0039] 2. Seedling stage management: Same as in Example 2.

[0040] 3. Spraying treatment: For five-week-old seedlings growing under normal and controlled watering conditions, the foliar spray prepared in Example 1 of this invention was applied. Before spraying, seedlings treated with different methods were separated using plastic wrap to avoid cross-contamination. Spraying was performed once daily, and every other day (between 18:00 and 19:00 in the evening, at 27 mL / m²). 2 Spray the above mixture).

[0041] The control group solution was prepared by adding 200 μL of 40 mM AEO aqueous solution to 40 mL of aqueous solution.

[0042] The effects of foliar spraying on normal wheat growth, such as Figure 4 As shown in the figure, under normal conditions, the wheat treated with the formulation grew better than the wheat treated with the control group.

[0043] The effects of foliar spraying on wheat drought resistance, such as Figure 5 As shown, under drought conditions, the wheat treated with the spray formulation exhibits significantly better drought resistance than the wheat treated with the control group.

[0044] Effects of foliar spray formulation on proline and malondialdehyde content in wheat leaves under drought conditions, such as Figure 6 As shown, the spray formulation treatment can significantly improve the drought resistance of wheat, increase the proline content in wheat leaves under drought conditions, and decrease the malondialdehyde content in wheat leaves under drought conditions.

[0045] Example 4: Improving the drought resistance of millet by using plant foliar sprays 1. Planting of seedling crops: Same as in Example 2.

[0046] 2. Seedling stage management: Same as in Example 2.

[0047] 3. Spraying treatment: For five-week-old seedlings growing under normal and controlled watering conditions, the foliar spray prepared in Example 1 of this invention was applied. Before spraying, seedlings treated with different methods were separated using plastic wrap to avoid cross-contamination. Spraying was performed once daily, and every other day (between 18:00 and 19:00 in the evening, at 27 mL / m²). 2 Spray the above mixture).

[0048] The control group solution was prepared by adding 200 μL of 40 mM AEO aqueous solution to 40 mL of aqueous solution.

[0049] The effects of foliar spraying on the normal growth of millet, such as Figure 7 As shown, under normal conditions, the millet treated with the spray formula grew better than the millet treated with the control group.

[0050] The effect of foliar spraying on the drought resistance of millet, such as Figure 8 As shown, under drought conditions, the millet treated with the spray formulation exhibits significantly better drought resistance than the millet treated with the control group.

[0051] Effects of foliar spray formulation on proline and malondialdehyde content in millet leaves under drought conditions, such as Figure 9 As shown, the spray formulation treatment can significantly improve the drought resistance of millet, increase the proline content in millet leaves under drought conditions, and decrease the malondialdehyde content in millet leaves under drought conditions.

Claims

1. The application of a plant foliar spray in improving the drought resistance of crops; characterized in that: The plant foliar spray is a mixed solution of fatty alcohol polyoxyethylene ether and soybean saponin Bb. In the mixed solution, the ratio of the fatty alcohol polyoxyethylene ether to the soybean saponin Bb is 1 mmol: 2.5~5.0 mg.

2. The application according to claim 1, characterized in that: The solvent used in the mixed solution is water.

3. The application according to claim 1 or 2, characterized in that: In the mixed solution, the molar concentration of the fatty alcohol polyoxyethylene ether is 0.2~0.5 mM.

4. The application according to claim 3, characterized in that: The crops mentioned include soybeans, wheat, and millet.

5. The application according to claim 4, characterized in that: The dosage of the plant foliar spray is 20-30 mL / m². 2 .

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