Rumen-protected vitamin complex and method for preparing the same

Abstract

The present application relates to a kind of rumen protection complex vitamin and its preparation method.The complex vitamin includes vitamin, polyethylene glycol glyceryl ricinoleate, carboxymethyl cellulose, antioxidant, adhesive and fatty acid, polyethylene glycol glyceryl ricinoleate accounts for 0.1~1.5% of the total mass of vitamin and fatty acid, carboxymethyl cellulose accounts for 1~5% of the total mass of vitamin and fatty acid.The present application is coated by using fatty acid to vitamin, fatty acid can protect vitamin to pass through rumen safely, reduce the degradation of vitamin in rumen;By adding polyethylene glycol glyceryl ricinoleate and carboxymethyl cellulose, the release rate of vitamin in stomach or small intestine is improved, so the complex vitamin of the present application has lower degradation rate in rumen, while having higher release rate in stomach and intestine.

Description

Technical Field

[0001] This invention relates to the field of compound vitamin technology, specifically to a rumen-protecting compound vitamin and its preparation method. Background Technology

[0002] Vitamins are a class of organic compounds essential for the nutrition and growth of ruminants, playing a vital role in metabolism, growth, development, and health. A deficiency in any vitamin can cause physiological dysfunction and lead to disease, preventing animals from achieving optimal health and production performance. However, vitamins are unstable in the rumen of ruminants, with a high degradation rate. Studies have shown that the rumen significantly degrades multivitamins; only 30-60% of added multivitamins reach the small intestine, with the remainder being degraded in the rumen. This not only results in vitamin waste but can also cause vitamin deficiencies or poor vitamin utilization in ruminants.

[0003] Therefore, rumen protection is essential to mitigate vitamin degradation in the rumen and improve vitamin utilization. Furthermore, ensuring vitamins are released and absorbed in the abomasum and small intestine is equally important. Summary of the Invention

[0004] The purpose of this invention is to provide a rumen-protective complex vitamin that has a lower degradation rate in the rumen while having a higher release rate in the abomasum.

[0005] Another objective of this invention is to provide a method for preparing a rumen-protecting complex vitamin, which is simple and convenient.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A rumen-protecting complex vitamin, comprising vitamins, polyethylene glycol glycerol ricinoleate, carboxymethyl cellulose, antioxidants, binders, and fatty acids, wherein the polyethylene glycol glycerol ricinoleate accounts for 0.1 to 1.5% of the total mass of the vitamins and fatty acids.

[0008] This invention utilizes fatty acid coating to encapsulate vitamins. Fatty acids protect vitamins as they pass safely through the rumen, reducing their degradation there. However, excessive coating can hinder vitamin release in the abomasum or small intestine, impairing absorption. The inventors further improved vitamin release in the abomasum and small intestine by adding polyethylene glycol glycerol ricinoleate and combining it with carboxymethyl cellulose, thereby improving both rumen degradation and abomasum / small intestine release rates. Therefore, the complex vitamins of this invention exhibit lower degradation rates in the rumen and higher release rates in the abomasum.

[0009] Preferably, the polyethylene glycol glycerol ricinoleate accounts for 0.2 to 1.5% of the total mass of the vitamins and fatty acids.

[0010] More preferably, the polyethylene glycol glycerol ricinoleate accounts for 0.5% to 1% of the total mass of the vitamins and fatty acids, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%.

[0011] In some embodiments, the polyethylene glycol glycerol ricinoleate is selected from one or more of Bredol 683, Bredol 693, and Bredol 694.

[0012] Preferably, the carboxymethyl cellulose accounts for 1 to 5% of the total mass of the vitamins and the fatty acids.

[0013] More preferably, the carboxymethyl cellulose accounts for 2-4% of the total mass of the vitamins and the fatty acids.

[0014] Preferably, based on 100% of the total mass of the complex vitamins, the complex vitamins comprise:

[0015] Vitamins 30-40%,

[0016] Polyethylene glycol glycerol ricinoleate 0.2-1.5%,

[0017] Carboxymethyl cellulose 2-4%,

[0018] Antioxidant 0.01-0.1%,

[0019] Adhesive 0.5-2%,

[0020] Fatty acids 55-65%.

[0021] Preferably, the fatty acid is stearic acid.

[0022] More preferably, the stearic acid is octadecanoic acid.

[0023] Preferably, the antioxidant is selected from butylated hydroxytoluene and / or 2-tert-butylhydroquinone. The combination of the two is more effective than using butylated hydroxytoluene or 2-tert-butylhydroquinone alone.

[0024] Preferably, the vitamins include one or more of vitamin A, vitamin D3, and vitamin E.

[0025] Preferably, the vitamin A includes one or more of vitamin A palmitate, vitamin A acetate, and vitamin A propionate. Esterified vitamin A has higher stability.

[0026] Preferably, the adhesive is one or more of natural attapulgite and natural bentonite.

[0027] The present invention also provides a method for preparing the rumen-protecting complex vitamin as described above, the method comprising mixing other components except fatty acids to form a mixture, heating the fatty acids to a liquid state, then mixing them with the mixture to form an emulsion, and spray granulating the emulsion.

[0028] Preferably, the outlet temperature of the spray is controlled to be -20 to 20°C. Low-temperature spraying can prevent vitamins from being damaged by heat.

[0029] In some embodiments, the preparation method includes the following steps:

[0030] Step 1: Weigh out the vitamins, polyethylene glycol glycerol ricinoleate, carboxymethyl cellulose, antioxidants and binders according to the formula, mix them and heat to 50-60℃;

[0031] Step 2: Weigh the fatty acids according to the formula, heat them to 60-80°C, and then mix them with the mixture from Step 1 to form an emulsion;

[0032] Step 3: The emulsion from Step 2 is fed into a centrifugal spray granulator for granulation to produce compound vitamins.

[0033] The compound vitamins of this invention are coated or encapsulated with fatty acids, which makes the compound vitamins resistant to the pH in the rumen and prevents them from being decomposed by microorganisms, thus providing good protection for the compound vitamins. At the same time, fatty acids can also provide energy for ruminants. Furthermore, by combining fatty acids with polyethylene glycol glycerol ricinoleate and carboxymethyl cellulose, the release of compound vitamins in the abomasum or intestine can be promoted, thereby improving the absorption and utilization rate of compound vitamins.

[0034] Using the compound vitamins of this invention, the nutritional needs of ruminants can be met while requiring a lower dosage.

[0035] The preparation method of this invention uses low-temperature spraying, which can avoid the destruction of vitamins by temperature, and at the same time, the preparation process is shorter and more effective.

[0036] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art:

[0037] This invention uses fatty acids to coat vitamins, which can protect vitamins and ensure their safe passage through the rumen, reducing vitamin degradation in the rumen. By adding polyethylene glycol glycerol ricinoleate and carboxymethyl cellulose, the release rate of vitamins in the abomasum or small intestine is improved. Therefore, the compound vitamins of this invention have a lower degradation rate in the rumen and a higher release rate in the abomasum and intestine. Detailed Implementation

[0038] The present invention will be further described below with reference to embodiments. However, the present invention is not limited to the following embodiments. The implementation conditions used in the embodiments can be further adjusted according to different requirements of specific applications, and the implementation conditions not specified are conventional conditions in the industry. The technical features involved in the various embodiments of the present invention can be combined with each other as long as they do not conflict with each other.

[0039] Unless otherwise specified, all raw materials used in the following examples and comparative examples are commercially available products. Vitamin A palmitate (oil) was purchased from Zhejiang Xinhecheng, 1.7 million IU / g; Vitamin D3 (oil) was purchased from Zhejiang Xinhecheng, 5 million IU / g; Vitamin E (oil) was purchased from Zhejiang Xinhecheng, with a content ≥98%; the active ingredient of Bredol 683 is polyethylene glycol glycerol ricinoleate.

[0040] Unless otherwise specified, the binder used in the following examples and comparative examples is natural attapulgite.

[0041] Examples 1-3 and Comparative Examples 1-2

[0042] A method for preparing a rumen-protecting complex vitamin includes the following steps:

[0043] Step 1: Weigh out vitamin A palmitate (oil), vitamin D3 (oil), vitamin E (oil), Bredol 683, carboxymethyl cellulose, BHT (butylated hydroxytoluene) and binder according to the formula, mix them, and heat in a water bath to 50-60°C.

[0044] Step 2: Weigh out octadecanoic acid according to the formula, heat it in a water bath to 60-75℃, and then mix it with the mixture from Step 1 above to form an emulsion;

[0045] Step 3: The emulsion from Step 2 is fed into a centrifugal spray granulator for granulation, and the outlet air temperature of the spray is controlled at 0±5℃ to produce compound vitamins.

[0046] The formulations of the compound vitamin raw materials for each embodiment and comparative example are shown in Table 1 below.

[0047] Table 1

[0048] Components Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Vitamin A palmitate / g 8 8 8 8 8 Vitamin D3 / g 3 3 3 3 3 Vitamin E / g 24 24 24 24 24 Bredol 683 / g 0.7 1.4 0.35 0.7 0 Carboxymethyl cellulose / g 3 3 3 0 3 BHT / g 0.03 0.03 0.03 0.03 0.03 adhesives / g 1 1 1 1 1 Octadecanic acid / g 60.27 59.57 60.62 63.27 60.97

[0049] Performance testing:

[0050] The degradation rates of the compound vitamins prepared in the above examples and comparative examples were tested in abomasal and rumen fluids, respectively. The test methods were based on the article "The Effect of Rumen Passage on Coated Vitamins and Amino Acids and Their Impact on Dairy Cow Production Performance" by Zhang Chen and Zhang Guiguo, College of Animal Science and Technology, Shandong Agricultural University, 2016.

[0051] The test results of the compound vitamins prepared in the above embodiments and comparative examples are shown in Table 2.

[0052] Table 2

[0053] Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Degradation rate (true gastric juice, average value) 93.20％ 88.6％ 88.50％ 88.40％ 88.00％ Degradation rate (rumen fluid, average value) 16.20％ 16.5％ 16.50％ 17.00％ 16.80％

[0054] As shown in Table 2, the compound vitamins prepared in Example 1 of this invention exhibited the lowest degradation rate in rumen fluid and the highest degradation rate in gastric fluid. Conversely, with increasing Bredol 683 content, the degradation rate in gastric fluid decreased, while the degradation rate in rumen fluid increased slightly. Furthermore, with decreasing Bredol 683 content, the degradation rate in gastric fluid also decreased, while the degradation rate in rumen fluid increased. Preferably, Bredol 683 accounts for 0.3% to 1% of the total mass of the raw materials.

[0055] Furthermore, the combined addition of Bredol 683 and carboxymethyl cellulose, compared to adding them separately, better protects the product from degradation in rumen fluid and facilitates its release in gastric juice.

[0056] The present invention has been described in detail above, with the aim of enabling those skilled in the art to understand and implement the invention. However, this description should not be construed as limiting the scope of protection of the invention. All equivalent changes or modifications made in accordance with the spirit and essence of the invention should be included within the scope of protection of the invention.

Claims

1. A rumen-protecting complex vitamin, characterized in that: Based on the total mass of the complex vitamins as 100%, the complex vitamins comprise: Vitamins 30-40%, Polyethylene glycol glycerol ricinoleate 0.5-1%, Carboxymethyl cellulose 2-4%, Antioxidant 0.01~0.1%, Adhesive 0.5~2%, Fatty acids 55-65%.

2. The rumen-protecting complex vitamin according to claim 1, characterized in that: The fatty acid is octadecanoic acid.

3. The rumen-protecting complex vitamin according to claim 1, characterized in that: The antioxidant is selected from butylated hydroxytoluene and / or 2-tert-butylhydroquinone.

4. The rumen-protecting complex vitamin according to claim 1, characterized in that: The vitamins include one or more of vitamin A, vitamin D3, and vitamin E.

5. The rumen-protecting complex vitamin according to claim 4, characterized in that: The vitamin A includes one or more of vitamin A palmitate, vitamin A acetate, and vitamin A propionate.

6. The rumen-protecting complex vitamin according to claim 1, characterized in that: The adhesive is one or more of natural attapulgite and natural bentonite.

7. The method for preparing rumen-protecting complex vitamins as described in any one of claims 1 to 6, characterized in that: The preparation method includes mixing other components besides fatty acids to form a mixture, heating the fatty acids to a liquid state, then mixing them with the mixture to form an emulsion, and spray granulating the emulsion.

8. The method for preparing the rumen-protecting complex vitamin according to claim 7, characterized in that: The outlet temperature of the spray is controlled to be -20~20℃.