An anti-aging repair dressing containing four composite active factors and a preparation method thereof

Abstract

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

Description

Technical Field

[0001] This invention relates to the field of medical dressings and skin repair materials, and particularly to an anti-aging and repair dressing containing four complex active factors and its preparation method. Background Technology

[0002] With age, environmental stimuli (such as ultraviolet radiation and pollution), and post-medical aesthetic procedures, the skin is prone to aging and barrier damage, manifesting as fine lines, sagging, dryness, sensitivity, and slow wound healing, severely impacting skin health and appearance. Currently, anti-aging and repair dressings, as a common type of medical care product, are widely used in skin care and post-medical aesthetic procedures repair. Their core function lies in achieving skin barrier reconstruction, collagen regeneration, and antioxidant effects through the action of active ingredients.

[0003] In existing technologies, anti-aging repair dressings mostly employ a combination of single or dual active factors, resulting in limited efficacy and poor synergy, making it difficult to simultaneously meet multiple needs such as skin anti-aging, barrier repair, and anti-inflammatory soothing. For example, dressings containing only collagen can promote collagen regeneration but lack antioxidant and anti-inflammatory effects; dressings containing ectoine focus on anti-irritation but cannot effectively address skin laxity. Furthermore, the matrix materials of existing dressings are mostly ordinary hydrophilic fibers with few surface polar groups, resulting in weak adsorption capacity for active factors and easy loss of these factors, thus reducing the dressing's repair effect. In addition, the active factors have poor stability and are easily deactivated during preparation, further affecting the dressing's efficacy.

[0004] Furthermore, in existing preparation methods, unreasonable methods of adding active factors and lyophilization processes can lead to uneven distribution of active factors and significant inactivation, preventing them from fully exerting their biological activity. Therefore, developing an anti-aging and repair dressing with multiple synergistic anti-aging and repair effects, high stability of active factors, and strong matrix adsorption, along with its preparation method, has become a pressing technical problem to be solved in this field. Summary of the Invention

[0005] In view of this, the present invention proposes an anti-aging and repair dressing containing four composite active factors and its preparation method to solve the above problems.

[0006] The technical solution of the present invention is implemented as follows: an anti-aging and repair dressing containing a quadruple composite active factor, comprising the following raw materials in parts by weight: 75-90 parts of matrix material, 5-15 parts of quadruple composite active factor, and 3-8 parts of moisturizing auxiliary agent; the quadruple composite active factor is composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine and dipotassium glycyrrhizate, and the weight ratio of the four is (3-6):(1-3):(2-4):(1-2); the matrix material is a hydrophilic nonwoven fabric or bio-fiber membrane material that has been surface-grafted and activated by low-temperature plasma, and its surface is introduced with carboxyl and / or hydroxyl polar groups.

[0007] Furthermore, the low-temperature plasma surface grafting activation treatment is performed using a radio frequency plasma processor, with the working gas being a mixture of argon and oxygen in a volume ratio of 1:5~10, and the treatment is carried out at a pressure of 30~60Pa and a power of 80~150W for 60~120s.

[0008] Furthermore, the recombinant human type III collagen has a molecular weight of 50-70 kDa and a purity of not less than 95%.

[0009] Furthermore, the moisturizing aid comprises glyceryl polyether-26, panthenol, and sodium hyaluronate in a mass ratio of (2~5):(0.5~2):(0.1~0.5).

[0010] The preparation method of the above-mentioned anti-aging and repair dressing includes the following steps: S1. Ectoin is mixed and stirred with low molecular weight sodium hyaluronate with a molecular weight of 5~10kDa at 25~35℃ to form a complex. S2. Recombinant human type III collagen, acetyl hexapeptide-8, dipotassium glycyrrhizate and the complex obtained in step S1 are dissolved in deionized water pre-cooled to 10-15°C in proportion, and stirred until completely dissolved to obtain an active solution. S3. Add the moisturizing auxiliary agent to the remaining deionized water, heat to 45~50℃ and homogenize for 5~10 minutes. After cooling to 25~30℃, add it to the active liquid in step S2 and mix evenly to obtain the wetting solution. S4. The matrix material treated with low-temperature plasma is completely immersed in the wetting solution and subjected to vacuum negative pressure adsorption treatment. The vacuum degree is -0.06~-0.08MPa and the treatment time is 15~20 minutes. S5. Place the adsorbed matrix material in a constant temperature environment of 4~8℃ for static annealing treatment for 30~60 minutes. S6. The annealed material is subjected to light-proof low-temperature freeze-drying treatment. During the freeze-drying process, nitrogen gas is introduced every 60 minutes in combination with a vacuum pulse to raise the pressure of the freeze-drying chamber from 10Pa to 500Pa and maintain it for 10 seconds before restoring the vacuum. This process is repeated 3 to 5 times. The freeze-drying endpoint temperature is controlled at -40℃ to obtain the dressing.

[0011] Furthermore, the amount of low molecular weight sodium hyaluronate used in step S1 is 0.3 to 0.8 times the weight of ectoine.

[0012] Furthermore, the amount of deionized water used in step S2 is 80-85% of the total impregnation liquid mass, and the remaining deionized water in step S3 is 15-20% of the total impregnation liquid mass.

[0013] Furthermore, in step S3, the stirring speed is 150~200 rpm, and the pH of the solution is maintained at 5.8~6.5 during the mixing process.

[0014] Furthermore, the low-temperature freeze-drying process described in step S5 adopts a programmed cooling mode: in the first stage, the temperature is lowered to -20°C at a rate of 0.8~1.2°C / min and held for 1~3 hours; in the second stage, the temperature is lowered to -40°C at a rate of 0.3~0.8°C / min and held for 3~5 hours.

[0015] The above-mentioned anti-aging and repair dressings are used in the preparation of medical devices for improving skin aging and repair.

[0016] Compared with the prior art, the beneficial effects of the present invention are: This invention employs a quadruple composite active factor composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate. The four components work synergistically: recombinant human type III collagen promotes collagen regeneration and improves skin laxity; acetyl hexapeptide-8 inhibits neurotransmitter release and reduces fine lines; ectoine resists environmental stimuli and protects skin cells; and dipotassium glycyrrhizate has anti-inflammatory and soothing effects, reducing skin sensitivity. This achieves four effects: anti-aging, repair, anti-inflammation, and anti-irritation, solving the problem of single-function dressings in existing products.

[0017] This invention involves low-temperature plasma surface grafting activation treatment of the matrix material, introducing carboxyl and / or hydroxyl polar groups onto its surface. This significantly enhances the matrix material's adsorption capacity for active factors, reduces the loss of active factors, and simultaneously improves the hydrophilicity and biocompatibility of the matrix material, making the dressing adhere more closely to the skin and promoting the absorption of active ingredients.

[0018] This invention optimizes the preparation process by using steps such as complexing ectoin with low molecular weight sodium hyaluronate, pre-cooling and dissolving the active factors, vacuum negative pressure adsorption, low-temperature annealing, and nitrogen-assisted vacuum pulse freeze drying to maximize the preservation of the bioactivity of the active factors and ensure the repair effect of the dressing. At the same time, it makes the components evenly distributed, improving the stability and shelf life of the dressing.

[0019] The dressing of this invention contains no irritating ingredients, has a pH value consistent with the physiological pH value of human skin, has good biocompatibility, and is suitable for all skin types, especially for the care of sensitive skin, wounds after medical aesthetic procedures, and other fragile skin. It can be prepared into products to improve skin aging and repair, and has broad application prospects. Detailed Implementation

[0020] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0021] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0022] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0023] Example 1 An anti-aging and repair dressing containing four-fold composite active factors includes the following raw materials in parts by weight: 75 parts of hydrophilic nonwoven fabric, 5 parts of four-fold composite active factors, and 3 parts of moisturizing aid. The quadruple composite active factor is composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine and dipotassium glycyrrhizate, with a weight ratio of 3:1:2:1. The moisturizing aids include glyceryl polyether-26, panthenol, and sodium hyaluronate in a mass ratio of 2:0.5:0.1; The hydrophilic nonwoven fabric is subjected to low-temperature plasma surface grafting activation treatment. The treatment parameters are as follows: a radio frequency plasma processor is used, the working gas is a mixture of argon and oxygen with a volume ratio of 1:5, and the treatment is carried out at 30Pa pressure and 80W power for 60s.

[0024] The preparation method of the above-mentioned anti-aging and repair dressing includes the following steps: S1. Ectoin and low molecular weight sodium hyaluronate, in an amount 0.8 times the weight of ectoin, are mixed and stirred at 35°C to form a complex. S2. Recombinant human type III collagen, acetyl hexapeptide-8, dipotassium glycyrrhizate and the complex obtained in step S1 are dissolved in deionized water pre-cooled to 15°C in proportion. The amount of deionized water is 85% of the total mass of the wetting solution. Stir until completely dissolved to obtain the active solution. S3. Add the moisturizing auxiliary agent to the remaining deionized water, heat to 50°C and homogenize for 10 minutes. After cooling to 30°C, add it to the active solution in step S2 and mix evenly with a stirring speed of 200 rpm. Keep the pH of the solution at 6.5 to obtain the wetting solution. S4. The hydrophilic nonwoven fabric treated with low-temperature plasma is completely immersed in the impregnation liquid and subjected to vacuum negative pressure adsorption treatment. The vacuum degree is -0.08MPa and the treatment time is 20 minutes. S5. The adsorbed hydrophilic nonwoven fabric is placed in a constant temperature environment of 8℃ for static annealing for 60 minutes. S6. The annealed material is subjected to light-proof low-temperature freeze-drying treatment. During the freeze-drying process, nitrogen gas is introduced every 60 minutes in combination with a vacuum pulse to raise the pressure in the freeze-drying chamber from 10 Pa to 500 Pa and maintain it for 10 seconds before restoring the vacuum. This process is repeated 5 times. The freeze-drying adopts a programmed cooling mode: in the first stage, the temperature is lowered to -20°C at 1.2°C / min and held for 3 hours; in the second stage, the temperature is lowered to -40°C at 0.8°C / min and held for 5 hours. The final freeze-drying temperature is controlled to be -40°C, thus obtaining the dressing.

[0025] Example 2 An anti-aging and repair dressing containing four-fold composite active factors includes the following raw materials in parts by weight: 90 parts of bio-cellulose membrane material, 15 parts of four-fold composite active factors, and 8 parts of moisturizing auxiliary agent. The quadruple composite active factor is composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine and dipotassium glycyrrhizate, with a weight ratio of 6:3:4:2. The moisturizing aids include glyceryl polyether-26, panthenol, and sodium hyaluronate in a mass ratio of 5:2:0.5; The bio-fiber membrane material is subjected to low-temperature plasma surface grafting activation treatment. The treatment parameters are as follows: a radio frequency plasma processor is used, the working gas is a mixture of argon and oxygen with a volume ratio of 1:10, and the treatment is carried out at 150W power for 120s under a pressure of 60Pa.

[0026] The preparation method of the above-mentioned anti-aging and repair dressing includes the following steps: S1. Ectoin and low molecular weight sodium hyaluronate, in an amount 0.3 times the weight of ectoin, are mixed and stirred at 25°C to form a complex. S2. Recombinant human type III collagen, acetyl hexapeptide-8, dipotassium glycyrrhizate and the complex obtained in step S1 are dissolved in deionized water pre-cooled to 10°C in proportion. The amount of deionized water is 80% of the total mass of the wetting solution. Stir until completely dissolved to obtain the active solution. S3. Add the moisturizing auxiliary agent to the remaining deionized water, which is 20% of the total wetting solution mass. Heat to 45°C and homogenize for 5 minutes. After cooling to 25°C, add it to the active solution in step S2 and mix evenly with a stirring speed of 150 rpm. Keep the pH of the solution at 5.8 to obtain the wetting solution. S4. The bio-cellulose membrane material treated with low-temperature plasma is completely immersed in the wetting solution and subjected to vacuum negative pressure adsorption treatment. The vacuum degree is -0.06MPa and the treatment time is 15 minutes. S5. Place the adsorbed bio-fiber membrane material in a constant temperature environment of 4℃ for static annealing treatment for 30 minutes. S6. The annealed material is subjected to light-proof low-temperature freeze-drying treatment. During the freeze-drying process, nitrogen gas is introduced every 60 minutes in combination with a vacuum pulse to raise the pressure in the freeze-drying chamber from 10 Pa to 500 Pa and maintain it for 10 seconds before restoring the vacuum. This process is repeated 3 times. The freeze-drying adopts a programmed cooling mode: in the first stage, the temperature is lowered to -20°C at 0.8°C / min and maintained for 1 hour; in the second stage, the temperature is lowered to -40°C at 0.3°C / min and maintained for 3 hours. The final freeze-drying temperature is controlled to be -40°C, thus obtaining the dressing.

[0027] Example 3 An anti-aging and repair dressing containing four complex active factors includes the following raw materials in parts by weight: 82 parts of matrix material, 10 parts of four complex active factors, and 5 parts of moisturizing aid. The quadruple composite active factor is composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine and dipotassium glycyrrhizate in a weight ratio of 5:2:3:1.5. The moisturizing aids include glyceryl polyether-26, panthenol, and sodium hyaluronate in a mass ratio of 2:1.5:0.3; The hydrophilic nonwoven fabric is subjected to low-temperature plasma surface grafting activation treatment. The treatment parameters are as follows: a radio frequency plasma processor is used, the working gas is a mixture of argon and oxygen with a volume ratio of 1:8, and the treatment is carried out at 50 Pa pressure and 100 W power for 80 s.

[0028] The preparation method of the above-mentioned anti-aging and repair dressing includes the following steps: S1. Ectoin and low molecular weight sodium hyaluronate, in an amount 0.5 times the weight of ectoin, are mixed and stirred at 30°C to form a complex. S2. Recombinant human type III collagen, acetyl hexapeptide-8, dipotassium glycyrrhizate and the complex obtained in step S1 are dissolved in deionized water pre-cooled to 12°C in proportion. The amount of deionized water is 82% of the total mass of the wetting solution. Stir until completely dissolved to obtain the active solution. S3. Add the moisturizing auxiliary agent to the remaining deionized water, which is 18% of the total wetting solution mass. Heat to 48°C and homogenize for 8 minutes. After cooling to 28°C, add it to the active solution in step S2 and mix evenly with a stirring speed of 180 rpm. Keep the pH of the solution at 6.2 to obtain the wetting solution. S4. The hydrophilic nonwoven fabric treated with low-temperature plasma is completely immersed in the impregnation liquid and subjected to vacuum negative pressure adsorption treatment. The vacuum degree is -0.07MPa and the treatment time is 18 minutes. S5. The adsorbed hydrophilic nonwoven fabric is placed in a constant temperature environment of 6℃ for 45 minutes for static annealing. S6. The annealed material is subjected to light-proof low-temperature freeze-drying treatment. During the freeze-drying process, nitrogen gas is introduced every 60 minutes in combination with a vacuum pulse to raise the pressure in the freeze-drying chamber from 10 Pa to 500 Pa and maintain it for 10 seconds before restoring the vacuum. This process is repeated 4 times. The freeze-drying adopts a programmed cooling mode: in the first stage, the temperature is lowered to -20°C at 1.0°C / min and held for 2 hours; in the second stage, the temperature is lowered to -40°C at 0.5°C / min and held for 4 hours. The final freeze-drying temperature is controlled to be -40°C, thus obtaining the dressing.

[0029] Comparative Example 1 The difference between this comparative example and Example 3 is that the quadruple recombinant active factor lacks dipotassium glycyrrhizate, and the weight ratio of the other three active factors, recombinant human type III collagen, acetyl hexapeptide-8, and ectoine, is adjusted to 5:2:3, with the total weight parts remaining at 10 parts; the amounts of other raw materials and the preparation process are the same as in Example 3.

[0030] Comparative Example 2 The difference between this comparative example and Example 3 is that the weight ratio of the four-fold composite active factor is 7:0.5:1:3, and the total weight is still 10 parts; the amount of other raw materials and the preparation process are the same as in Example 3.

[0031] Comparative Example 3 The difference between this comparative example and Example 3 is that the hydrophilic nonwoven fabric was not subjected to low-temperature plasma surface grafting activation treatment and was directly used for subsequent wetting and adsorption; the amount of other raw materials and the preparation process are the same as in Example 3.

[0032] Comparative Example 4 The difference between this comparative example and Example 3 is that the complexation of ectoin with low molecular weight sodium hyaluronate in step S1 is omitted, and ectoin is directly added to pre-cooled deionized water along with recombinant human type III collagen, acetyl hexapeptide-8, and dipotassium glycyrrhizate for dissolution; the remaining raw material amounts and preparation processes are the same as in Example 3.

[0033] Comparative Example 5 The difference between this comparative example and Example 3 is that nitrogen gas combined with vacuum pulse was not introduced in the freeze-drying process in step S6. Instead, a conventional freeze-drying process was used, directly cooling the temperature to -40°C and holding it for 4 hours. The remaining raw material amounts and preparation processes are the same as in Example 3.

[0034] Effect test The anti-aging and repair dressings prepared in Examples 1-3 and Comparative Examples 1-5 were verified by in vitro cell experiments. The key steps are as follows: 1. Cell culture: Human immortalized keratinocytes (HaCaT cells) were selected and seeded in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin antibiotics. They were then cultured in a constant temperature incubator at 37°C and 5% CO2. When the cell confluence reached 80%~90%, they were passaged for use. 2. Model Construction: Passaged HaCaT cells were seeded into 96-well plates at a density of 5 × 10⁶ cells per well. 3 After culturing individual cells for 24 hours to allow cell adhesion, ultraviolet light (UVB, dose 30 mJ / cm²) was used. 2 A skin barrier damage and aging model was constructed by irradiation. After irradiation, the model was cultured for 12 hours to confirm successful model construction. 3. Preparation of dressing extract: The dressings of each example and comparative example were cut into small pieces and added to sterile PBS buffer at a material-to-liquid ratio of 1:10 (g / mL). The mixture was then placed in a constant temperature shaker at 37℃ and 120rpm for 24 hours. After filtration through a 0.22μm filter membrane for sterilization, dressing extracts of different concentrations (10%, 20%, 50%) were obtained. Sterile PBS buffer was used as a blank control. 4. Experimental grouping and treatment: The constructed model cells were divided into a blank control group, Example 1-3 groups, and Comparative Example 1-5 groups. Each group had 3 parallel wells. The corresponding concentration (50%, the optimal concentration was determined by preliminary experiments) of dressing extract was added to the corresponding group. An equal volume of sterile PBS buffer was added to the blank control group. The cells were incubated in a 37°C, 5% CO2 incubator for 48 hours. 5. Indicator Detection: After culture, cell proliferation rate was detected using the CCK-8 assay, and collagen secretion and inflammatory factor (IL-6) content in the cell supernatant were detected using an ELISA kit. The IL-6 inhibition rate was calculated to evaluate the anti-aging and repair efficacy of the dressing. The calculation formula is as follows: Cell proliferation rate (%) = (Absorbance value of experimental group - Absorbance value of blank control group) / (Absorbance value of blank control group) × 100% IL-6 inhibition rate (%) = (IL-6 content in blank control group - IL-6 content in experimental group) / (IL-6 content in blank control group) × 100% The results are shown in the table below:

[0035] The test results above show that the anti-aging and repair dressings prepared in Examples 1-3 of this invention have significantly better effects on promoting the proliferation of HaCaT cells, promoting collagen secretion, and inhibiting inflammatory factors than the comparative examples. Specific analysis is as follows: Compared with Example 3, the lack of dipotassium glycyrrhizate in Comparative Example 1 resulted in a decrease in IL-6 inhibition rate and a significant reduction in collagen secretion, indicating that the integrity of the quadruple composite active factors is the key to achieving synergistic anti-inflammatory and repair-promoting effects. Compared with Example 3, when the ratio of active factors deviated from the scope of the present invention, the cell proliferation rate, collagen secretion and IL-6 inhibition rate all decreased significantly, indicating that only the specific ratio of the four-fold compound active factors can fully exert the synergistic anti-aging and repair effects. Compared with Example 3, Comparative Example 3 showed that the active factors were severely lost after the matrix was not activated, and all detection indicators decreased significantly. This indicates that low-temperature plasma activation treatment can significantly improve the adsorption capacity of the matrix for active factors and ensure that the active factors can play an effective role. Compared with Example 3, Comparative Example 4 showed that without the ectoine complexation step, ectoine activity was inactivated, and cell proliferation rate and IL-6 inhibition rate decreased, indicating that this step can effectively protect the biological activity of ectoine and enhance its cell protection and anti-stimulation effects. Compared with Example 3, the conventional freeze-drying process caused the active factors to be inactivated and the matrix structure to be destroyed, resulting in a decrease in various efficacy indicators. This shows that the nitrogen-vacuum pulse freeze-drying process can retain the activity of the active factors and the integrity of the matrix structure to the maximum extent, thus ensuring the efficacy of the dressing.

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An anti-aging and repair dressing containing four composite active factors, characterized in that, The raw materials include the following parts by weight: 75-90 parts of matrix material, 5-15 parts of quadruple composite active factor, and 3-8 parts of moisturizing auxiliary agent; the quadruple composite active factor is composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine and dipotassium glycyrrhizate, and the weight ratio of the four is (3-6):(1-3):(2-4):(1-2); the matrix material is a hydrophilic nonwoven fabric or bio-fiber membrane material that has been surface-grafted and activated by low-temperature plasma.

2. The anti-aging and repair dressing as described in claim 1, characterized in that, The low-temperature plasma surface grafting activation treatment uses a radio frequency plasma processor. The working gas is a mixture of argon and oxygen with a volume ratio of 1:5~10. The treatment is carried out at a pressure of 30~60Pa and a power of 80~150W for 60~120s.

3. The anti-aging and repair dressing as described in claim 1, characterized in that, The recombinant human type III collagen has a molecular weight of 50-70 kDa and a purity of not less than 95%.

4. The anti-aging and repair dressing as described in claim 1, characterized in that, The moisturizing aids include glyceryl polyether-26, panthenol, and sodium hyaluronate in a mass ratio of (2~5):(0.5~2):(0.1~0.5).

5. The method for preparing the anti-aging and repair dressing according to any one of claims 1-4, characterized in that, Includes the following steps: S1. Ectoin is mixed and stirred with low molecular weight sodium hyaluronate with a molecular weight of 5~10kDa at 25~35℃ to form a complex. S2. Recombinant human type III collagen, acetyl hexapeptide-8, dipotassium glycyrrhizate and the complex obtained in step S1 are dissolved in deionized water pre-cooled to 10-15°C in proportion, and stirred until completely dissolved to obtain an active solution. S3. Add the moisturizing auxiliary agent to the remaining deionized water, heat to 45~50℃ and homogenize for 5~10 minutes. After cooling to 25~30℃, add it to the active liquid in step S2 and mix evenly to obtain the wetting solution. S4. The matrix material treated with low-temperature plasma is completely immersed in the wetting solution and subjected to vacuum negative pressure adsorption treatment. The vacuum degree is -0.06~-0.08MPa and the treatment time is 15~20 minutes. S5. Place the adsorbed matrix material in a constant temperature environment of 4~8℃ for static annealing treatment for 30~60 minutes. S6. The annealed material is subjected to light-proof low-temperature freeze-drying treatment. During the freeze-drying process, nitrogen gas is introduced every 60 minutes in combination with a vacuum pulse to raise the pressure of the freeze-drying chamber from 10Pa to 500Pa and maintain it for 10 seconds before restoring the vacuum. This process is repeated 3 to 5 times. The freeze-drying endpoint temperature is controlled at -40℃ to obtain the dressing.

6. The preparation method according to claim 5, characterized in that, The amount of low molecular weight sodium hyaluronate used in step S1 is 0.3 to 0.8 times the weight of ectoine.

7. The preparation method according to claim 5, characterized in that, The amount of deionized water used in step S2 is 80-85% of the total impregnation liquid mass, and the remaining deionized water in step S3 is 15-20% of the total impregnation liquid mass.

8. The preparation method according to claim 5, characterized in that, In step S3, the stirring speed is 150~200 rpm, and the pH of the solution is maintained at 5.8~6.5 during the mixing process.

9. The preparation method according to claim 5, characterized in that, The low-temperature freeze-drying process described in step S5 adopts a programmed cooling mode: the first stage cools down to -20℃ at a rate of 0.8~1.2℃ / min and holds for 1~3 hours; the second stage cools down to -40℃ at a rate of 0.3~0.8℃ / min and holds for 3~5 hours.

10. The use of the anti-aging and repair dressing as described in any one of claims 1-4 in the preparation of a medical device for improving skin aging and repair.

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