A traditional Chinese medicine composition for preventing post-traumatic stress disorder and a preparation method thereof

Abstract

The application provides a traditional Chinese medicine composition for preventing post-traumatic stress disorder and a preparation method thereof. The traditional Chinese medicine composition for preventing post-traumatic stress disorder comprises the following raw materials in parts by weight: 10-20 parts of ginseng and 10-20 parts of rhizoma polygonati, and in addition, 15-25 parts of mulberry, 15-25 parts of hawthorn and 5-15 parts of raphanus sativus. The traditional Chinese medicine composition is reasonably matched, has the effects of nourishing heart and soothing nerves, benefiting qi and nourishing blood, invigorating spleen and benefiting stomach and can well play a role in preventing post-traumatic stress disorder.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, and in particular to a traditional Chinese medicine composition for preventing post-traumatic stress disorder and its preparation method. Background Technology

[0002] Post-traumatic stress disorder (PTSD) is a high-risk mental illness. With the occurrence of traumatic events, the incidence of PTSD is increasing year by year, causing a significant social and economic burden. PTSD is characterized by increased vigilance, flashbacks, stress avoidance, difficulty in erasing fearful memories, and is accompanied by depressive and anxiety symptoms. Currently, the treatment of PTSD mainly relies on antidepressants; sertraline and paroxetine are the only first-line drugs approved by the FDA for the treatment of PTSD.

[0003] However, in current treatments, patients tend to avoid situations related to their traumatic experiences, leading to drug abuse and consequently cognitive and memory impairments, resulting in a persistently high lifetime prevalence. Furthermore, the use of sertraline and paroxetine can cause numerous adverse reactions of varying degrees. For example, paroxetine's main adverse reactions involve the central nervous system (including drowsiness, insomnia, agitation, tremor, anxiety, and dizziness), the gastrointestinal system (including constipation, nausea, diarrhea, dry mouth, vomiting, and flatulence), and others include fatigue. Even if Western medicines have clear efficacy, they still cannot simultaneously treat or eliminate the core symptoms of PTSD.

[0004] PTSD occurs due to traumatic events. Epidemiological studies show that the incidence rate in the general population is 6%, while after a traumatic event, the incidence rate rises to as high as 35%. Therefore, developing relevant antagonistic drugs is of great significance for reducing the sequelae of PTSD and improving patients' quality of life. Furthermore, after a major traumatic event, there is a latency period (approximately 1-3 months) before PTSD-like symptoms gradually appear, and this latency period offers hope for the prevention and treatment of PTSD. Although Traditional Chinese Medicine (TCM) has no records related to PTSD, finding drugs to control this borderline state and thus addressing the symptoms, thereby leveraging TCM's concept of "prevention before disease occurs," is particularly important.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The purpose of this invention is to provide a traditional Chinese medicine composition for preventing post-traumatic stress disorder and its preparation method. The traditional Chinese medicine composition is rationally formulated and has the effects of nourishing the heart and calming the mind, replenishing qi and blood, and strengthening the spleen and stomach. It can effectively prevent post-traumatic stress disorder.

[0007] This invention provides a traditional Chinese medicine composition for preventing post-traumatic stress disorder, comprising the following raw materials in parts by weight: 10-20 parts ginseng, 10-20 parts polygonatum; preferably, the traditional Chinese medicine composition further comprises the following raw materials in parts by weight: 15-25 parts mulberry, 15-25 parts hawthorn and 5-15 parts radish seed.

[0008] Furthermore, the traditional Chinese medicine composition of the present invention comprises the following raw materials in parts by weight: 20 parts ginseng and 10 parts polygonatum; preferably, the traditional Chinese medicine composition further comprises the following raw materials in parts by weight: 20 parts mulberry, 20 parts hawthorn and 10 parts radish seed.

[0009] Traditional Chinese medicine (TCM) considers PTSD to fall under the category of emotional disorders, often caused by extreme emotions such as grief, fear, and shock. Symptoms commonly include insomnia, anxiety, easy startling, palpitations, and timidity, indicative of deficiency of heart and gallbladder qi. TCM methods can alleviate these symptoms and prevent further disease progression through prevention and intervention. Ginseng can benefit the qi of the heart and gallbladder, calm a disordered mind, and is used in many formulas for improving heart and gallbladder qi deficiency. The inventors have also confirmed ginseng's preventative effect on PTSD in previous studies. However, ginseng is warming in nature, and patients with heart and gallbladder qi deficiency often experience depression due to deficiency, which can lead to internal heat; therefore, prolonged use is not advisable. Thus, finding TCM herbs that can synergistically enhance the effects of ginseng while also mitigating its warming and drying properties is crucial for developing TCM products for the intervention of critical states in PTSD.

[0010] After extensive research, the inventors discovered that combining Polygonatum sibiricum with ginseng significantly enhances the preventive effect against PTSD compared to using the same dose of ginseng or Polygonatum sibiricum alone. Polygonatum enters the spleen, lung, and kidney meridians, and can invigorate qi and nourish yin, replenish kidney essence, nourish brain marrow, benefit brain function and intelligence, and effectively prevent and treat nervous system diseases. When combined with ginseng, Polygonatum not only enhances the tonifying and calming effects of ginseng, but also counteracts the warming and drying properties of ginseng. Based on the combination of Polygonatum and ginseng, the inventors added mulberry, hawthorn, and radish seed. Mulberry has the effects of nourishing yin and blood, promoting body fluid production, and moistening the intestines. It can also regulate immunity, reduce inflammation, and provide antioxidant effects. Hawthorn has the effects of promoting digestion, strengthening the stomach, regulating qi and dispersing blood stasis, and reducing turbidity and lipids. It can promote digestion and lower blood pressure and lipids. Radish seed has the effects of relieving indigestion and bloating, reducing qi and resolving phlegm, and can also promote digestion. The above three herbs can invigorate the spleen and stomach to prevent the greasy taste of ginseng and Polygonatum from hindering the stomach. They also have a sour and sweet taste to harmonize the flavor and optimize the texture. In summary, this invention, by combining ginseng and polygonatum and adding mulberry, hawthorn, and radish seed, provides a new approach for the development of traditional Chinese medicine compositions for the prevention of PTSD.

[0011] The present invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition for preventing post-traumatic stress disorder, comprising the following steps:

[0012] S1: Ginseng and Polygonatum are pulverized, sieved, and mixed according to the weight parts to obtain ginseng and Polygonatum powder;

[0013] S2: Ginseng and Polygonatum powder was sequentially subjected to immersion extraction, ultrasonic extraction, and water decoction extraction, and then dried to obtain ginseng and Polygonatum extract;

[0014] Furthermore, the preparation method of the traditional Chinese medicine composition also includes step S3: mixing and pulverizing ginseng and polygonatum extract with mulberry, hawthorn and radish seeds in parts by weight to obtain a traditional Chinese medicine composition for preventing post-traumatic stress disorder.

[0015] In step S1 above, the sieving is done through a 30-40 mesh sieve.

[0016] In step S2 above, ginseng and polygonatum powder is soaked in distilled water for extraction; the weight ratio of ginseng and polygonatum powder to distilled water is controlled at 1:(15-25), for example 1:20; the extraction time is 1.5-2.5h, for example 2h.

[0017] In step S2 above, the extract after immersion extraction is subjected to ultrasonic extraction under ultrasonic conditions; the ultrasonic frequency is controlled at 40-60kHz, for example 50kHz; the ultrasonic extraction time is 0.5-1.5h, for example 1h.

[0018] In step S2 above, the water decoction extraction includes: a first decoction of the extract after ultrasonic extraction, followed by filtration to obtain a first filtrate and a first residue; a second decoction of distilled water added to the first residue, followed by filtration to obtain a second filtrate; and concentration of the first and second filtrates. The first decoction involves heating the extract after ultrasonic extraction to a boil and then simmering it over low heat for 30-50 minutes, for example, 40 minutes. The second decoction involves heating the mixture of the first residue and distilled water to a boil and then simmering it over low heat for 20-40 minutes, for example, 30 minutes. The weight ratio of the first residue to distilled water is 1:(10-20), for example, 1:15.

[0019] In the traditional Chinese medicine composition of this invention, ginseng nourishes the five internal organs, calms the mind, stabilizes the spirit, and stops palpitations; polygonatum enters the spleen, lung, and kidney meridians, can replenish qi and yin, nourish the kidneys and replenish essence, nourish the brain marrow, benefit the brain and enhance intelligence, and effectively prevent and treat nervous system diseases; the combination of polygonatum and ginseng not only enhances the tonifying and calming effects of ginseng, but also counteracts the warming and drying properties of ginseng, which is beneficial for improving immunity and regulating qi and blood. Meanwhile, mulberry has the effects of nourishing yin and blood, generating fluids and moistening dryness, and can regulate immunity, anti-inflammation, and anti-oxidation; hawthorn has the effects of promoting digestion and strengthening the stomach, promoting qi circulation and dispersing blood stasis, and resolving turbidity and lowering lipids, which can accelerate blood circulation, promote digestion, and lower blood pressure and lipids; the combination of hawthorn and mulberry has the effect of regulating cardiovascular function. In addition, radish seed has the effects of promoting digestion and relieving bloating, lowering qi and resolving phlegm, and can also promote digestion and improve sleep; the above three herbs can strengthen the spleen and stomach to prevent the greasy and cloying nature of ginseng and polygonatum from hindering the stomach, and all have a sour and sweet taste to harmonize the flavor and optimize the texture. Through the rational combination of the above components, the combination achieves the effects of nourishing the heart and calming the mind, replenishing qi and blood, and strengthening the spleen and stomach, thus effectively preventing PTSD. In particular, mulberry, hawthorn, and radish seeds can improve the taste of the traditional Chinese medicine composition and reduce the unpleasant taste of the product. The preparation method of the above-mentioned traditional Chinese medicine composition for preventing PTSD is simple to operate, the quality is controllable, and the conversion and utilization rate of medicinal materials is improved. Attached Figure Description

[0020] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0021] Figure 1 The preparation process flow charts for various traditional Chinese medicine compositions are shown.

[0022] Figure 2 HPLC chromatogram of methanol;

[0023] Figure 3 This is the HPLC detection chromatogram of the mixed standard;

[0024] Figure 4 The HPLC chromatogram of PF1 is shown.

[0025] Figure 5 The HPLC chromatogram of PF2;

[0026] Figure 6 The HPLC chromatogram of PF3 is shown.

[0027] Figure 7 The HPLC chromatogram of PF4;

[0028] Figure 8The results show the opening arm time ratio of the elevated cross maze; where "**" and "***" indicate statistical differences compared to SPS.

[0029] Figure 9 This represents the total distance traveled by mice in the open field experiment.

[0030] Figure 10 The results show the distance of the center of activity of mice in the open field experiment; where "**", "***", and "****" indicate statistical differences compared with SPS.

[0031] Figure 11 The results show the activity center time of mice in the open field experiment; where "*" and "**" indicate statistical differences compared to SPS.

[0032] Figure 12 The results show the number of activity centers of mice in the open field experiment; where "*" and "***" indicate statistical differences compared to SPS.

[0033] Figure 13 Statistical results of fear memory retardation time during re-exposure; where "*" indicates a statistically significant difference between SPS+PF3 and SPS;

[0034] Figure 14 This is a statistical result of the time lag in fear memory during the extinction phase; where "**", "***", and "****" indicate statistical differences compared to SPS.

[0035] Figure 15 CORT kit standard curve (R) 2 >0.999);

[0036] Figure 16 The CORT content in the blood of mice in each group is shown; where "****" indicates a statistically significant difference compared with SPS.

[0037] Figure 17 Standard curve for IL-10 kit (R) 2 >0.99);

[0038] Figure 18 The values ​​represent the IL-10 levels in the blood of mice in each group; where "****" indicates a statistically significant difference compared to SPS.

[0039] Figure 19 The standard curve for the IL-1β kit (R) 2 >0.999);

[0040] Figure 20 The values ​​represent the IL-1β levels in the blood of mice in each group; where "**" indicates a statistically significant difference compared to SPS. Detailed Implementation

[0041] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0042] It should be noted that the terminology used herein is for the purpose of describing particular implementations only and is not intended to limit the exemplary implementations according to this application. As used herein, the singular form includes the plural form unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this description, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0043] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] Example 1

[0045] Combination Figure 1 As shown, the preparation method of the traditional Chinese medicine composition for preventing post-traumatic stress disorder in this embodiment includes the following steps:

[0046] 1. Preparation of ginseng and polygonatum freeze-dried powder

[0047] 1) Weigh out ginseng and polygonatum in proportion to 20 parts by weight and 10 parts by weight respectively. The total weight of ginseng and polygonatum is 30 parts by weight, and the weight ratio of ginseng to polygonatum is 2:1.

[0048] 2) Weigh out the ginseng and polygonatum separately and crush them. After crushing, pass them through a 30-40 mesh sieve and then mix them to obtain ginseng and polygonatum powder.

[0049] 3) Extraction by maceration: Place ginseng and polygonatum powder in an extraction tank, add 20 times the weight of distilled water, and perform maceration extraction at room temperature for 2 hours.

[0050] 4) Ultrasonic extraction: After the immersion extraction is completed, the extraction vessel is placed in an ultrasonic instrument for further ultrasonic extraction. The ultrasonic frequency is set to 50kHz and the ultrasonic extraction time is 1h.

[0051] 5) Water decoction extraction: The herbal powder and extract after ultrasonic extraction are further extracted by water decoction. The extraction includes a first decoction and a second decoction. In the first decoction, the mixture is heated to a boil and then simmered for 40 minutes. After filtration, the first filtrate is obtained, and the first residue is collected. In the second decoction, 15 times the weight of distilled water is added to the first residue, heated to a boil, and then simmered for 30 minutes. After filtration, the second filtrate is obtained. The first and second filtrates are mixed and concentrated to obtain ginseng and polygonatum extract with a concentration of 1 g / mL.

[0052] 6) Place the ginseng and polygonatum extract in a freeze dryer and freeze dry for 48 hours to obtain ginseng and polygonatum freeze-dried powder (denoted as PF3).

[0053] 2. Preparation of Traditional Chinese Medicine Compositions

[0054] Take 20 parts by weight of ginseng and 10 parts by weight of Polygonatum sibiricum freeze-dried powder, add 20 parts by weight of mulberry, 20 parts by weight of hawthorn and 10 parts by weight of radish seed, pulverize and mix well to obtain a traditional Chinese medicine composition for preventing post-traumatic stress disorder.

[0055] Example 2

[0056] 1. Preparation of ginseng and polygonatum freeze-dried powder

[0057] 1) Weigh out ginseng and polygonatum in proportion to 10 parts by weight and 20 parts by weight respectively. The total weight of ginseng and polygonatum is 30 parts by weight, and the weight ratio of ginseng to polygonatum is 1:2.

[0058] 2) Weigh out the ginseng and polygonatum separately and crush them. After crushing, pass them through a 30-40 mesh sieve and then mix them to obtain ginseng and polygonatum powder.

[0059] 3) Extraction by maceration: Place ginseng and polygonatum powder in an extraction tank, add 20 times the weight of distilled water, and perform maceration extraction at room temperature for 2 hours.

[0060] 4) Ultrasonic extraction: After the immersion extraction is completed, the extraction vessel is placed in an ultrasonic instrument for further ultrasonic extraction. The ultrasonic frequency is set to 50kHz and the ultrasonic extraction time is 1h.

[0061] 5) Water decoction extraction: The herbal powder and extract after ultrasonic extraction are further extracted by water decoction. The extraction includes a first decoction and a second decoction. In the first decoction, the mixture is heated to a boil and then simmered for 40 minutes. After filtration, the first filtrate is obtained, and the first residue is collected. In the second decoction, 15 times the weight of distilled water is added to the first residue, heated to a boil, and then simmered for 30 minutes. After filtration, the second filtrate is obtained. The first and second filtrates are mixed and concentrated to obtain ginseng and polygonatum extract with a concentration of 1 g / mL.

[0062] 6) Place the ginseng and polygonatum extract in a freeze dryer and freeze dry for 48 hours to obtain ginseng and polygonatum freeze-dried powder (denoted as PF4).

[0063] 2. Preparation of Traditional Chinese Medicine Compositions

[0064] Take 10 parts by weight of ginseng and 20 parts by weight of Polygonatum sibiricum freeze-dried powder, add 20 parts by weight of mulberry, 20 parts by weight of hawthorn and 10 parts by weight of radish seed, pulverize and mix well to obtain a traditional Chinese medicine composition for preventing post-traumatic stress disorder.

[0065] Example 3

[0066] 1. Preparation of ginseng and polygonatum freeze-dried powder

[0067] 1) Weigh out ginseng and polygonatum in 15 parts by weight and 15 parts by weight respectively. The total weight of ginseng and polygonatum is 30 parts by weight.

[0068] 2) Weigh out the ginseng and polygonatum separately and crush them. After crushing, pass them through a 30-40 mesh sieve and then mix them to obtain ginseng and polygonatum powder.

[0069] 3) Extraction by maceration: Place ginseng and polygonatum powder in an extraction tank, add 15 times the weight of distilled water, and perform maceration extraction at room temperature for 1.5 hours.

[0070] 4) Ultrasonic extraction: After the immersion extraction is completed, the extraction vessel is placed in an ultrasonic instrument for further ultrasonic extraction. The ultrasonic frequency is set to 40kHz and the ultrasonic extraction time is 1.5h.

[0071] 5) Water decoction extraction: The herbal powder and extract after ultrasonic extraction are further extracted by water decoction. The extraction includes a first decoction and a second decoction. In the first decoction, the mixture is heated to a boil and then simmered for 30 minutes. After filtration, the first filtrate is obtained, and the first residue is collected. In the second decoction, 10 times the weight of distilled water is added to the first residue, heated to a boil and then simmered for 20 minutes. After filtration, the second filtrate is obtained. The first and second filtrates are mixed and concentrated to obtain ginseng and polygonatum extract with a concentration of 1 g / mL.

[0072] 6) Place the ginseng and polygonatum extract in a freeze dryer and freeze-dry for 48 hours to obtain ginseng and polygonatum freeze-dried powder.

[0073] 2. Preparation of Traditional Chinese Medicine Compositions

[0074] Take 15 parts by weight of ginseng and 15 parts by weight of polygonatum freeze-dried powder, add 15 parts by weight of mulberry, 25 parts by weight of hawthorn and 5 parts by weight of radish seed, pulverize and mix well to obtain a traditional Chinese medicine composition for preventing post-traumatic stress disorder.

[0075] Example 4

[0076] 1. Preparation of ginseng and polygonatum freeze-dried powder

[0077] 1) Weigh out 20 parts by weight of ginseng and 10 parts by weight of polygonatum, and the total weight of ginseng and polygonatum is 30 parts by weight.

[0078] 2) Weigh out the ginseng and polygonatum separately and crush them. After crushing, pass them through a 30-40 mesh sieve and then mix them to obtain ginseng and polygonatum powder.

[0079] 3) Extraction by maceration: Place ginseng and polygonatum powder in an extraction tank, add 25 times the weight of distilled water, and perform maceration extraction at room temperature for 2.5 hours.

[0080] 4) Ultrasonic extraction: After the immersion extraction is completed, the extraction vessel is placed in an ultrasonic instrument for further ultrasonic extraction. The ultrasonic frequency is set to 60kHz and the ultrasonic extraction time is 0.5h.

[0081] 5) Water decoction extraction: The herbal powder and extract after ultrasonic extraction are further extracted by water decoction. The extraction includes a first decoction and a second decoction. In the first decoction, the mixture is heated to a boil and then simmered for 50 minutes. After filtration, the first filtrate is obtained, and the first residue is collected. In the second decoction, 20 times the weight of distilled water is added to the first residue, heated to a boil and then simmered for 40 minutes. After filtration, the second filtrate is obtained. The first and second filtrates are mixed and concentrated to obtain ginseng and polygonatum extract with a concentration of 1 g / mL.

[0082] 6) Place the ginseng and polygonatum extract in a freeze dryer and freeze-dry for 48 hours to obtain ginseng and polygonatum freeze-dried powder.

[0083] 2. Preparation of Traditional Chinese Medicine Compositions

[0084] Take 20 parts by weight of ginseng and 10 parts by weight of polygonatum freeze-dried powder, add 25 parts by weight of mulberry, 15 parts by weight of hawthorn and 15 parts by weight of radish seed, pulverize and mix well to obtain a traditional Chinese medicine composition for preventing post-traumatic stress disorder.

[0085] Compare with Example 1

[0086] This comparative example is basically the same as Example 1 except that no Polygonatum was added; ginseng freeze-dried powder (denoted as PF1) and traditional Chinese medicine composition were prepared respectively.

[0087] Compare with Example 2

[0088] This comparative example is basically the same as Example 1 except that ginseng is not added; freeze-dried Polygonatum sibiricum powder (denoted as PF2) and traditional Chinese medicine composition were prepared respectively.

[0089] Experimental Example 1

[0090] The contents of ginsenoside Rg1 and ginsenoside Rb1 in the lyophilized powders PF1-PF4 of Control Examples 1-2 and Examples 1-2 were determined by HPLC. The mobile phase was acetonitrile-water; the reference solvent was methanol; and the detection wavelength was 203 nm. Simultaneously, the polysaccharide content in the lyophilized powders was determined by the phenol-sulfuric acid method. For specific procedures, refer to the "Content Determination" section under "Ginseng" and "Polygonatum" in Part I of the 2020 edition of the Chinese Pharmacopoeia.

[0091] Test results are shown Figures 2 to 7 And Table 1, Figures 2 to 5 In the text, "1" represents ginsenoside Rg1, and "2" represents ginsenoside Rb1.

[0092] Table 1. Results of determination of ginsenoside and polysaccharide content in various freeze-dried powders

[0093] Traditional Chinese medicine composition Ginsenoside Rg1 (μg / g) Ginsenoside Rb1 (μg / g) Polysaccharides (mg / g) PF1 49.99±0.09 25.42±1.75 34.33±0.79 PF3 30.12±0.06 18.58±0.13 58.83±0.50 PF4 16.48±0.19 10.67±0.36 60.11±0.57 PF2 0 0 52.48±0.35

[0094] Experimental Example 2

[0095] The elevated cross maze test and open field test were used to evaluate the effects of lyophilized powders PF1-PF4 from Controls 1-2 and Examples 1-2, as well as paroxetine hydrochloride (PRX), on anxiety and depression symptoms in SPS mice. The fear memory extinction test was used to detect fear memory symptoms in SPS mice. Immediately after SPS modeling, the mice were administered the lyophilized powders and PRX once daily for 14 consecutive days at 0.1 g / time. The results are as follows:

[0096] 1) Elevated cross-maze experiment:

[0097] The opening arm time ratio of the elevated cross-maze is calculated as follows:

[0098] Open arm time ratio % = Open arm activity time / Total experimental time 300s × 100%.

[0099] See results Figure 8 ; Figure 8 The results showed that PF3 and the western medicine paroxetine hydrochloride (PRX) had the same effect, significantly increasing the time spent in open arms in SPS mice and reducing anxiety-like behaviors.

[0100] 2) Open field experiment

[0101] Under conditions of unimpaired motor function (total distance), center distance, center time, and center entries were compared. The results are shown below. Figures 9 to 12 .

[0102] The results showed that PF3 was more effective and similar to paroxetine hydrochloride in improving anxiety and depression symptoms, but it was superior to paroxetine hydrochloride in promoting the extinction of fear memories.

[0103] 3) Fear memory extinction test:

[0104] The lag time of SPS mice during the re-exposure and resolution phases was statistically analyzed, and the results are shown in the table below. Figure 13 , Figure 14 .

[0105] The results showed that PF3 significantly promoted the extinction of fear memories in SPS mice. Compared with paroxetine hydrochloride, PF3 showed a statistically significant difference, indicating that PF3 was more effective in improving fear memories.

[0106] 4) CORT biochemical index testing:

[0107] The CORT levels in the blood of mice in each group were measured, and the results are shown in the table below. Figure 15 , Figure 16 .

[0108] The results showed that SPS levels were higher than those in control mice, PF3 significantly reduced CORT levels in the blood of SPS mice, while PRX did not show a significant advantage in this experiment.

[0109] 5) Detection of anti-inflammatory factor indicators:

[0110] The level of the anti-inflammatory factor IL-10 was measured, and the results are shown below. Figure 17 , Figure 18 .

[0111] The results showed that the IL-10 level in the blood of SPS mice was significantly reduced, PF3 could significantly increase the IL-10 level in the blood of SPS mice, while PRX did not show a significant advantage in this experiment.

[0112] 6) Detection of pro-inflammatory factor indicators:

[0113] The levels of the pro-inflammatory factor IL-1β were measured, and the results are shown in the table below. Figure 19 , Figure 20 .

[0114] The results showed that the IL-1β level in the blood of SPS mice was significantly increased, PF3 could significantly reduce the IL-1β level in the blood of SPS mice, while PRX did not show a significant advantage in this experiment.

[0115] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing post-traumatic stress disorder, characterized in that, It is made of raw materials in the following weight parts: ginseng 20 parts, rhizoma polygonati 10 parts, mulberry 20 parts, hawthorn 20 parts and raphanus 10 parts; The preparation method of the traditional Chinese medicine composition comprises the following steps: S1: crushing, sieving and mixing ginseng and rhizoma polygonati according to weight parts to prepare ginseng rhizoma polygonati powder; S2: sequentially performing immersion extraction, ultrasonic extraction and water decocting extraction on the ginseng rhizoma polygonati powder, drying after extraction to prepare ginseng rhizoma polygonati extract; S3: mixing, crushing the ginseng rhizoma polygonati extract with mulberry, hawthorn and raphanus according to weight parts to prepare the traditional Chinese medicine composition for preventing post-traumatic stress disorder.

2. The preparation method of the traditional Chinese medicine composition for preventing post-traumatic stress disorder according to claim 1, characterized in that, The preparation method comprises the following steps: S1: crushing, sieving and mixing ginseng and rhizoma polygonati according to weight parts to prepare ginseng rhizoma polygonati powder; S2: sequentially performing immersion extraction, ultrasonic extraction and water decocting extraction on the ginseng rhizoma polygonati powder, drying after extraction to prepare ginseng rhizoma polygonati extract; S3: mixing, crushing the ginseng rhizoma polygonati extract with mulberry, hawthorn and raphanus according to weight parts to prepare the traditional Chinese medicine composition for preventing post-traumatic stress disorder.

3. The production method according to claim 2, characterized by, The sieving is sieving through a 30-40 mesh sieve.

4. The production method according to claim 2, characterized by, The ginseng rhizoma polygonati powder is immersed in distilled water for immersion extraction.

5. The production method according to claim 4, characterized by, The weight ratio of the ginseng rhizoma polygonati powder to the distilled water is controlled to be 1: (15-25) during the immersion extraction, and the immersion extraction time is 1.5-2.5 h.

6. The preparation method according to claim 2, characterized in that, Ultrasonic extraction is performed on the extracted liquid after the immersion extraction under ultrasonic conditions.

7. The production method according to claim 6, characterized by The ultrasonic frequency is controlled to be 40-60 kHz during the ultrasonic extraction, and the ultrasonic extraction time is 0.5-1.5 h.

8. The preparation method according to claim 2, characterized in that, The water decocting extraction comprises: performing first decocting on the extracted liquid after the ultrasonic extraction, filtering to obtain first filtrate and first residue after the first decocting; adding distilled water to the first residue to perform second decocting, filtering to obtain second filtrate after the second decocting; mixing the first filtrate and the second filtrate and then concentrating.

9. The production method according to claim 8, characterized by, The first decocting is to heat and boil the extracted liquid after the ultrasonic extraction and then to decoct with small fire for 30-50 min; the second decocting is to heat and boil the mixture of the first residue and distilled water and then to decoct with small fire for 20-40 min, wherein the weight ratio of the first residue to the distilled water is 1: (10-20).

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