A neuraminic acid composition for improving memory and learning functions and a preparation method thereof

Abstract

The present invention belongs to the technical field of nutritional products, and specifically relates to a neuraminic acid composition for improving memory and learning functions and a preparation method thereof. The present invention has developed a multi-component and multi-functional neuraminic acid composition, in which the various nutrients contained therein synergistically enhance the effect of improving memory and learning functions. By adding lysolecithin and resistant dextrin during the preparation of the neuraminic acid composition, a variety of functional components with poor stability and easy oxidation contained in the composition can be stabilized, including neuraminic acid, DHA, arachidonic acid, etc., so that the obtained neuraminic acid composition has a longer shelf life and also improves the bioavailability of the functional components in the composition.

Description

Technical Field

[0001] The present invention belongs to the technical field of nutritional products, and specifically relates to a neuraminic acid composition for improving memory and learning functions and a preparation method thereof. Background Art

[0002] Nervonic acid (NA) is a ω-9 long-chain unsaturated monoolefin fatty acid, also known as shark acid (Selacholeic Acid), chemical name cis-15-tetracosenic acid (cis-15-Tetracosenic Acid), molecular formula C 24 H 46 O2, molecular weight is 366.6. Nervonic acid is white flaky crystals at room temperature, insoluble in water, soluble in alcohols such as methanol and ethanol, with a melting point of 41-43°C. Nervonic acid was first discovered in mammalian nerve tissue, is an important component of biological membranes in nerve tissue, and is a signature component of the medulla in cerebrosides. It is mainly derived from shark brain and shark oil, and now a process for extracting or fermenting nervonic acid from natural plants has also been developed.

[0003] Neuroacid can completely pass through the blood-brain barrier and directly act on nerve fibers to repair and dredge them, regenerate damaged and detached protective sheaths, dissolve necrotic tissue that blocks the channels, induce self-growth and division of nerve fibers, and enable the information generated by nerve cells and the external world to be smoothly transmitted through the nerve fibers, thereby activating damaged, diseased and dormant nerve cells, reshaping the neural network, and restoring part or all of the patient's functions in language, memory, sensation, limbs, etc., to achieve complete recovery from brain disease.

[0004] Due to the good physiological activity of neuraminic acid and the fact that the human body is difficult to produce and can only be supplemented through external intake, neuraminic acid has become a new generation of health care product additive that is extremely popular both at home and abroad.

[0005] CN109288005A discloses a composition with the function of assisting in improving memory, which combines folic acid compounds, algae oil DHA powder, neuraminic acid and N-acetylneuraminic acid, and controls the weight ratio of various components so that different components can exert a synergistic effect on each other, thereby effectively improving reversible memory loss.

[0006] CN110200288A discloses a nutritional composition for infants and young children, which contains zinc, vitamin D, neuraminic acid and oil substances, which can increase the neuraminic acid content in the plasma of infants and young children, meet the needs of infants and young children for neuraminic acid, zinc and vitamin D, significantly improve memory, promote the healthy development of the infants and young children's nervous system, and further improve intestinal immune resistance by promoting immune cell transformation.

[0007] Despite the above studies, on the one hand, the compositions disclosed in these studies have simple ingredients and relatively single functions, and the effect on improving the memory and learning functions of infants and young children is relatively general. On the other hand, both neuraminic acid and DHA have the problems of poor stability and easy oxidation, resulting in a short shelf life. Summary of the invention

[0008] In order to solve the above problems, the present invention has developed a multi-component and multi-functional neuraminic acid composition, which has an excellent effect of improving memory and learning functions. The present invention also unexpectedly found that by adding hemolysin and resistant dextrin during the preparation process of the neuraminic acid composition, the obtained neuraminic acid composition has a longer shelf life and a certain improvement in bioavailability.

[0009] Specifically, the present invention provides a neuraminic acid composition for improving memory and learning functions, which comprises 20-25 parts of neuraminic acid, 7-9 parts of DHA, 1.5-2.5 parts of phosphatidylserine, 4-5 parts of arachidonic acid, 0.01-1 parts of walnut peptide, 6-7 parts of N-acetylneuraminic acid, 0.02-1 parts of taurine, 5-6 parts of isomaltooligosaccharide, 0.02-0.05 parts of lactase, 0.5-1.2 parts of peony seed oil peptide, 1-2 parts of L-tea theanine, 0.5-1 parts of magnesium, 0.05-0.1 parts of iron, 0.03-0.1 parts of zinc, 0.01-0.02 parts of vitamin B6, 18-20 parts of lysolecithin and 16-20 parts of resistant dextrin.

[0010] Furthermore, the neuraminic acid is derived from Acer truncatum seed oil.

[0011] Furthermore, the neuraminic acid composition is in the form of solid powder or emulsion.

[0012] Furthermore, the magnesium exists in the form of magnesium sulfate or magnesium lactate.

[0013] Furthermore, the iron exists in the form of ferrous sulfate or ferrous lactate.

[0014] Furthermore, the zinc exists in the form of zinc gluconate, zinc citrate or zinc lactate.

[0015] The present invention also provides a method for preparing the nervonic acid composition as described herein, comprising the following steps:

[0016] (1) Add lysolecithin and resistant dextrin to deionized water and stir until evenly dispersed.

[0017] (2) The solid powders of nervonic acid, DHA, phosphatidylserine, arachidonic acid, walnut peptide, N-acetylneuraminic acid, taurine, isomaltooligosaccharide, lactase, peony seed oil peptide, L-tea theanine, magnesium-containing substances, iron-containing substances, zinc-containing substances and vitamin B6 are uniformly mixed together, and then added to the mixed solution of step (1), and the mixture is stirred until uniformly dispersed.

[0018] (3) placing the mixed solution of step (2) in an emulsification tank, continuously introducing nitrogen or carbon dioxide from the bottom of the emulsification tank, emulsifying at 50-60° C. for 10-30 min under stirring, cooling to room temperature, and homogenizing to obtain an emulsion.

[0019] (4) The emulsion of step (3) is vacuum concentrated and sterilized to obtain the neuraminic acid composition, or the emulsion of step (3) is spray dried and sterilized using nitrogen or carbon dioxide as a drying medium to obtain the neuraminic acid composition.

[0020] Furthermore, in step (1), the amount of deionized water used is 5 times the mass of lysolecithin and resistant dextrin.

[0021] Furthermore, the magnesium-containing substance includes magnesium sulfate or magnesium lactate.

[0022] Furthermore, the iron-containing substance includes ferrous sulfate or ferrous lactate.

[0023] Furthermore, the zinc-containing substance includes zinc gluconate, zinc citrate or zinc lactate.

[0024] Furthermore, in step (3), the stirring speed is 2000-5000 r / min.

[0025] Furthermore, in step (3), the homogenization includes passing the emulsified liquid through a 100-mesh sieve and homogenizing the filtrate at 50° C. and 15 MPa for 10-30 min.

[0026] Furthermore, in step (4), the inlet air temperature of the spray drying is 100-200°C, and the outlet air temperature is 30-80°C.

[0027] Furthermore, in step (4), the sterilization includes pasteurization at 80-85° C. for 10-15 minutes.

[0028] The present invention also provides a use of the neuraminic acid composition described herein in formula milk powder.

[0029] Furthermore, the amount of the neuraminic acid composition described herein added to formula milk powder is 2-3 wt%.

[0030] Beneficial effects of the present invention

[0031] The present invention develops a multi-component and multi-functional neuraminic acid composition, in which various nutrients contained therein are synergistically enhanced, and together give the neuraminic acid composition of the present invention an excellent effect of improving memory and learning functions. In addition, the present invention also unexpectedly discovered that by adding lysolecithin and resistant dextrin during the preparation process of the neuraminic acid composition, a variety of functional components with poor stability and easy oxidation contained in the composition, including neuraminic acid, DHA, arachidonic acid, etc., can be stabilized, so that the obtained neuraminic acid composition has a longer shelf life, and further, it also improves the bioavailability of the functional components in the composition. DETAILED DESCRIPTION

[0032] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0033] Example 1

[0034] The present embodiment provides a neuraminic acid composition for improving memory and learning functions, which comprises, by weight, 20 parts of neuraminic acid, 9 parts of DHA, 2 parts of phosphatidylserine, 5 parts of arachidonic acid, 1 part of walnut peptide, 7 parts of N-acetylneuraminic acid, 1 part of taurine, 6 parts of isomaltooligosaccharide, 0.05 parts of lactase, 1 part of peony seed oil peptide, 2 parts of L-tea theanine, 1 part of magnesium, 0.1 part of iron, 0.1 part of zinc, 0.02 part of vitamin B6, 18 parts of lysolecithin and 16 parts of resistant dextrin.

[0035] The nervonic acid composition is prepared as follows:

[0036] (1) Add lysolecithin and resistant dextrin to 5 times the weight of deionized water and stir until evenly dispersed.

[0037] (2) The solid powders of nervonic acid, DHA, phosphatidylserine, arachidonic acid, walnut peptide, N-acetylneuraminic acid, taurine, isomaltooligosaccharide, lactase, peony seed oil peptide, L-tea theanine, magnesium-containing substances, iron-containing substances, zinc-containing substances and vitamin B6 are uniformly mixed together, and then added to the mixed solution of step (1), and the mixture is stirred until uniformly dispersed.

[0038] (3) The mixed solution of step (2) is placed in an emulsification tank, nitrogen is continuously introduced from the bottom of the emulsification tank, and emulsification is performed at 60° C. for 20 min under stirring at 2500 r / min, and the emulsified liquid is cooled to room temperature, and the emulsified liquid is passed through a 100-mesh sieve, and the filtrate is homogenized at 50° C. and 15 MPa for 30 min to obtain an emulsion.

[0039] (4) The emulsion of step (3) is spray dried with nitrogen as the drying medium, the inlet air temperature of the spray drying is 120°C, and the outlet air temperature is 45°C, and then pasteurized at 85°C for 15 minutes to obtain the neuraminic acid composition in the form of a solid powder.

[0040] Example 2

[0041] The present embodiment provides a neuraminic acid composition for improving memory and learning functions, which comprises, by weight, 25 parts of neuraminic acid, 7 parts of DHA, 2 parts of phosphatidylserine, 4 parts of arachidonic acid, 1 part of walnut peptide, 6 parts of N-acetylneuraminic acid, 0.05 parts of taurine, 5 parts of isomaltooligosaccharide, 0.05 parts of lactase, 1 part of peony seed oil peptide, 1 part of L-tea theanine, 0.5 parts of magnesium, 0.05 parts of iron, 0.05 parts of zinc, 0.01 parts of vitamin B6, 20 parts of lysolecithin and 18 parts of resistant dextrin.

[0042] The nervonic acid composition is prepared as follows:

[0043] (1) Add lysolecithin and resistant dextrin to 5 times the weight of deionized water and stir until evenly dispersed.

[0044] (2) The solid powders of nervonic acid, DHA, phosphatidylserine, arachidonic acid, walnut peptide, N-acetylneuraminic acid, taurine, isomaltooligosaccharide, lactase, peony seed oil peptide, L-tea theanine, magnesium-containing substances, iron-containing substances, zinc-containing substances and vitamin B6 are uniformly mixed together, and then added to the mixed solution of step (1), and the mixture is stirred until uniformly dispersed.

[0045] (3) placing the mixed solution of step (2) in an emulsification tank, continuously introducing nitrogen from the bottom of the emulsification tank, emulsifying at 60°C for 30 minutes under stirring at 3500 r / min, cooling to room temperature, passing the emulsified liquid through a 100-mesh sieve, and homogenizing the filtrate at 50°C and 15 MPa for 30 minutes to obtain an emulsion.

[0046] (4) The emulsion of step (3) is vacuum concentrated, and then pasteurized at 85° C. for 15 minutes to obtain the neuraminic acid composition in the form of an emulsion.

[0047] Comparative Example 1

[0048] This comparative example provides a neuraminic acid composition for improving memory and learning functions. The difference between it and Example 1 is that lysolecithin is replaced with lecithin, and the rest of the scheme is the same as Example 1.

[0049] Comparative Example 2

[0050] This comparative example provides a neuraminic acid composition for improving memory and learning functions. The difference between it and Example 1 is that lysolecithin is replaced by lecithin, and the amount of lecithin added is 30 parts by weight. The rest of the scheme is the same as Example 1.

[0051] Comparative Example 3

[0052] This comparative example provides a neuraminic acid composition for improving memory and learning functions. The difference between it and Example 1 is that lysolecithin is replaced by lecithin, and the amount of lecithin added is 60 parts by weight. The rest of the scheme is the same as Example 1.

[0053] Comparative Example 4

[0054] This comparative example provides a neuraminic acid composition for improving memory and learning functions, which differs from Example 1 in that it does not contain lysolecithin and resistant dextrin.

[0055] Test Example 1: Oxidative Stability Analysis of Nerve Acid Compositions

[0056] The neuraminic acid compositions prepared in Examples 1-2 and Comparative Examples 1-4 were placed in a constant temperature oven at 60°C, and samples were taken every 12 hours. The peroxide value of the samples was detected using a fat oxidation stability analyzer according to the titration method specified in GB5009.227-2016. Each sample was tested 3 times and the average value was taken. The results are shown in Table 1.

[0057] Table 1

[0058] Accelerated oxidation time (h) Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 0 0.00068 0.00069 0.00075 0.00072 0.00070 0.00081 12 0.00489 0.00524 0.00752 0.00604 0.00512 0.02689 24 0.01016 0.01129 0.01739 0.01286 0.01039 0.04876 36 0.02832 0.02992 0.03941 0.03392 0.03021 0.10385 48 0.04628 0.04923 0.11832 0.09873 0.05538 0.19652 60 0.10692 0.11271 0.20467 0.16492 0.12084 0.26847 72 0.12683 0.13552 0.25031 0.21793 0.14965 0.29751

[0059] It can be seen from the results in Table 1 that compared with Comparative Example 4 which does not contain lysolecithin and resistant dextrin, Examples 1-2 and Comparative Examples 1-3 can significantly inhibit the increase of peroxide value and have better oxidative stability, among which Example 1-2 has higher stability. Although the use of lecithin to replace lysolecithin also has the effect of enhancing oxidative stability, a larger amount (60 parts by weight) of lecithin needs to be added to achieve an oxidative stability close to that of Example 1-2. However, such a large amount of lecithin added is obviously not in compliance with relevant industry standards when the neuraminic acid composition of the present invention is used in formula milk powder.

[0060] Test Example 2: Bioavailability Test

[0061] The neuraminic acid compositions prepared in Examples 1-2 and Comparative Examples 1-4 were added at 3 wt % to skimmed milk powder without any additives under aseptic conditions and set aside.

[0062] 70 volunteers were selected for human trials on improving memory, among which the volunteers had not undergone similar experiments, had not taken medicines and health products related to improving memory, belonged to the same age group (16-18 years old), and had the same educational level (second-year high school students). The volunteers were divided into seven groups, each with 10 people. The gender composition, memory quotient mean and standard deviation of each group were basically the same. One group was randomly selected as the control group, and the other groups were experimental groups. The volunteers were treated with a double-blind method. The experimental group took skim milk powder prepared from the neuraminic acid composition of Examples 1-2 and Comparative Examples 1-4, respectively, and the control group took skim milk powder without any additives. The dosage and duration were 25g / 60kg per day for one month. The test method was carried out according to the Revised Wechsler Scale, and the test content included mind, recognition, association, picture and understanding. The results are shown in Table 2.

[0063] Table 2

[0064] Test items Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Control group Mind 31.34±4.22 32.11±4.17 31.56±4.31 30.94±4.74 32.54±4.01 31.23±4.43 31.51±4.25 Recognition 17.53±3.67 17.28±3.94 14.59±2.05 15.23±2.62 16.96±3.81 14.11±2.89 9.36±2.52 Lenovo 16.15±2.46 16.05±2.68 15.59±2.88 15.72±2.34 16.01±2.92 15.16±2.02 9.85±2.61 picture 16.83±1.86 16.89±2.04 15.52±1.42 15.91±1.25 16.53±1.33 15.13±2.03 8.97±2.31 understand 18.34±2.23 18.12±2.68 15.96±1.86 16.77±1.69 17.96±2.52 15.60±2.34 9.12±2.02

[0065] From the results in Table 2, it can be seen that the volunteers took the skimmed milk powder prepared by the nervonic acid composition of Examples 1-2 and Comparative Examples 1-4 for 30 days continuously. Compared with the control group, the scores of recognition, association, picture and understanding experiments were improved, indicating that the nervonic acid composition prepared by the present application can effectively assist in improving the memory of the volunteers by adding a variety of effective ingredients and controlling the content and mass ratio of various ingredients, and the volunteers had no adverse reactions during the period of taking. At the same time, it is also seen that compared with Comparative Example 4 which does not contain lysolecithin and resistant dextrin, the volunteers took the skimmed milk powder prepared by the nervonic acid composition of Examples 1-2 and Comparative Examples 1-3 continuously. The scores of recognition, association, picture and understanding experiments are also improved, which shows that the addition of lysolecithin and resistant dextrin can improve the bioavailability of the functional ingredients in the composition.

[0066] It should be noted that the preferred embodiments of the present invention are given in the specification of the present invention, but the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. These embodiments are not intended to be additional limitations on the content of the present invention. The purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. In addition, the above-mentioned technical features continue to be combined with each other to form various embodiments not listed above, which are all considered to be within the scope of the present invention specification; further, for ordinary technicians in this field, they can be improved or transformed according to the above description, and all these improvements and transformations should belong to the protection scope of the claims attached to the present invention.

Claims

1. A neuraminic acid composition for assisting in improving memory function, characterized in that: The composition is as follows by weight: 20-25 parts of nervonic acid, 7-9 parts of DHA, 1.5-2.5 parts of phosphatidylserine, 4-5 parts of arachidonic acid, 0.01-1 parts of walnut peptide, 6-7 parts of N-acetylneuraminic acid, 0.02-1 parts of taurine, 5-6 parts of isomaltooligosaccharide, 0.02-0.05 parts of lactase, 0.5-1.2 parts of peony seed oil peptide, 1-2 parts of L-tea theanine, 0.5-1 parts of magnesium, 0.05-0.1 parts of iron, 0.03-0.1 parts of zinc, 0.01-0.02 parts of vitamin B6, 18-20 parts of lysolecithin and 16-20 parts of resistant dextrin; The method of preparing the nervonic acid composition comprises the following steps: (1) Add lysolecithin and resistant dextrin to deionized water and stir until evenly dispersed. (2) The solid powders of nervonic acid, DHA, phosphatidylserine, arachidonic acid, walnut peptide, N-acetylneuraminic acid, taurine, isomaltooligosaccharide, lactase, peony seed oil peptide, L-tea theanine, magnesium-containing substances, iron-containing substances, zinc-containing substances and vitamin B6 are uniformly mixed together, and then added to the mixed solution of step (1), and the mixture is stirred until uniformly dispersed. (3) placing the mixed solution of step (2) in an emulsification tank, continuously introducing nitrogen or carbon dioxide from the bottom of the emulsification tank, emulsifying at 50-60° C. for 10-30 min under stirring, cooling to room temperature, and homogenizing to obtain an emulsion. (4) The emulsion of step (3) is vacuum concentrated and sterilized to obtain the neuraminic acid composition, or the emulsion of step (3) is spray dried and sterilized using nitrogen or carbon dioxide as a drying medium to obtain the neuraminic acid composition.

2. The neuraminic acid composition according to claim 1, characterized in that The nervonic acid composition is in the form of solid powder or emulsion.

3. A method for preparing the neuraminic acid composition according to any one of claims 1-2, characterized in that: The following steps are involved: (1) Add lysolecithin and resistant dextrin to deionized water and stir until evenly dispersed. (2) The solid powders of nervonic acid, DHA, phosphatidylserine, arachidonic acid, walnut peptide, N-acetylneuraminic acid, taurine, isomaltooligosaccharide, lactase, peony seed oil peptide, L-tea theanine, magnesium-containing substances, iron-containing substances, zinc-containing substances and vitamin B6 are uniformly mixed together, and then added to the mixed solution of step (1), and the mixture is stirred until uniformly dispersed. (3) placing the mixed solution of step (2) in an emulsification tank, continuously introducing nitrogen or carbon dioxide from the bottom of the emulsification tank, emulsifying at 50-60° C. for 10-30 min under stirring, cooling to room temperature, and homogenizing to obtain an emulsion. (4) The emulsion of step (3) is vacuum concentrated and sterilized to obtain the neuraminic acid composition, or the emulsion of step (3) is spray dried and sterilized using nitrogen or carbon dioxide as a drying medium to obtain the neuraminic acid composition.

4. The method according to claim 3, characterized in that In step (1), the amount of deionized water used is 5 times the mass of lysolecithin and resistant dextrin.

5. The method according to claim 3, characterized in that: The magnesium-containing substance includes magnesium sulfate or magnesium lactate, the iron-containing substance includes ferrous sulfate or ferrous lactate, and the zinc-containing substance includes zinc gluconate, zinc citrate or zinc lactate.

6. The method according to claim 3, characterized in that: In step (3), the stirring speed is 2000-5000 r / min.

7. The method according to claim 3, characterized in that In step (3), the homogenization includes passing the emulsified liquid through a 100-mesh sieve and homogenizing the filtrate at 50° C. and 15 MPa for 10-30 minutes.

8. The method according to claim 3, characterized in that In step (4), the inlet air temperature of the spray drying is 100-200°C, and the outlet air temperature is 30-80°C.

9. An application of the neuraminic acid composition as described in any one of claims 1-2 in formula milk powder.

10. The use according to claim 9, characterized in that The amount of the neuraminic acid composition added to the formula milk powder is 2-3 wt%.