Biomass-based antiviral agents, their preparation, and their application in inactivating plant viruses in aquatic bodies

Abstract

This invention belongs to the field of aquatic biological treatment and virus inactivation technology, providing a biomass-based antiviral agent and its preparation and application in the inactivation of aquatic plant viruses. The preparation method includes pretreating biomass, mixing the pretreated biomass with an activator to prepare activated carbon, mixing the activated carbon with a solution containing a nitrogen source and iron salt, calcining and carbonizing, cooling, acid washing, water washing, filtering, and drying to obtain the biomass-based antiviral agent. The nitrogen source is dicyandiamide and / or melamine; the iron salt is at least one selected from ferric chloride, ferric nitrate, ferric citrate, and ferric nitrate nonahydrate. The biomass-based antiviral agent prepared by this invention, as a catalyst in the inactivation of aquatic plant viruses, exhibits high inactivation rates and rapid inactivation speeds for viruses such as pepper mild mottle. It provides a low-cost, recyclable, and efficient biomass-based antiviral agent-catalyzed persulfate inactivation method for aquatic plant viruses, with excellent prospects for widespread application.

Description

Technical Field

[0001] This application relates to the fields of biological water treatment and virus inactivation technology, and in particular to a biomass-based antiviral agent and its preparation and application in the inactivation of plant viruses in aquatic bodies. Background Technology

[0002] Pepper Mild Mottle Virus (PMMoV) is a plant pathogenic virus that occurs in field-grown sweet peppers, chili peppers, and ornamental pepper varieties around the world. It is transmitted through soil and irrigation water and can cause stunted growth, resulting in a significant decrease in yield and quality.

[0003] Traditional PMMoV control technologies for water bodies mainly include chemical disinfection, ultraviolet disinfection, and biological control. However, these methods often suffer from problems such as unstable effectiveness, high operating costs, potential generation of toxic byproducts, and negative impacts on non-target organisms.

[0004] Peroxymonosulfate (PMS), as a strong oxidant, has been widely used in environmental engineering and water treatment. PMS can generate sulfate radicals (SO4·-) with high oxidizing power, which can effectively inactivate a variety of harmful microorganisms, including viruses. However, the activation of PMS usually requires high temperatures or ultraviolet radiation, which may not be practical or cost-effective in some applications.

[0005] To improve the activation efficiency of persulfate (PMS) and reduce the cost of PMMoV inactivation, researchers have begun exploring the use of catalysts to promote PMS activation. Catalysts can promote the decomposition of PMS under relatively low temperatures and light-free conditions, generating sulfate radicals, thereby efficiently inactivating PMMoV in water. Various catalysts, including metal oxides, metal-based hybrid materials, and photocatalysts, have shown potential in PMS activation and microbial inactivation.

[0006] There are many activation methods for persulfates, typically including transition metal activation, nonmetal doping, ultraviolet light activation, and ultrasound activation. Previous studies have found that transition metal activation is one of the most popular and effective methods, with transition metals including Co... 2+ Fe 2+ Mn 2+ Cu 2+ However, transition metal activation also has its drawbacks, such as the easy leakage of metal ions into the aquatic environment, leading to decreased catalytic performance and reusability, as well as secondary water pollution. In recent years, carbon materials have received widespread attention due to their abundant functional groups and high stability, but the activation effect of directly pyrolyzed biochar on persulfate is not satisfactory.

[0007] To address the aforementioned issues, existing research provides a method for preparing porous biochar using corn stalks as raw material and KHCO3 as a pore-forming and activating agent; using Co(NO3)2·6H2O and Fe(NO3)3·9H2O as graphitizing agents, CoFe2O4 as a precursor, and activated biochar as a carrier; and forming a graphitized composite material, CoFe2O4 / HPC, in situ at the heterogeneous interface between CoFe2O4 and activated biochar through high-temperature calcination under N2 atmosphere. The CoFe2O4 / HPC is then used to activate PMS. However, this CoFe2O4 / HPC composite material is suitable as a catalyst for degrading organic pollutants, but not for inactivating aquatic plants.

[0008] Existing research, such as the patent publication number CN116639789B, discloses a method for removing recalcitrant organic matter from wastewater using modified biochar catalysts to catalyze persulfate removal. This method involves mixing biochar with a solution containing iron and aluminum salts, adding a complexing agent to obtain a modified biochar precursor, and then calcining the modified biochar precursor to obtain the modified biochar catalyst. However, this modified biochar catalyst has a high metal content, and while it is suitable for activating persulfate to degrade organic matter in wastewater, it is not suitable for inactivating aquatic plants.

[0009] In conclusion, if a recyclable, green, and highly efficient catalyst can be developed to promote the activation of persulfate and achieve efficient, low-cost, and environmentally friendly inactivation of aquatic plant viruses, it will be more conducive to ensuring the health of agricultural products and the sustainable development of agriculture. Summary of the Invention

[0010] In view of the shortcomings of the prior art, the present invention provides a biomass-based antiviral agent, its preparation and application in the inactivation of aquatic plant viruses, to solve the problems of existing catalysts for activating persulfate being unsuitable for inactivating aquatic plant viruses; and the low efficiency and high cost of existing aquatic plant virus inactivation technologies.

[0011] To achieve the above and related objectives, the present invention adopts the following technical solution:

[0012] The first aspect of this invention provides a method for preparing a biomass-based antiviral agent, the method comprising:

[0013] (1) Pre-treat the biomass, mix the pre-treated biomass with an activator, and prepare activated carbon;

[0014] (2) Mix activated carbon with a solution containing nitrogen source and iron salt, and calcine and carbonize it; after calcine and carbonization, cool, acid wash, water wash, filter, and dry to obtain a biomass-based antiviral agent, wherein the nitrogen source is dicyandiamide and / or melamine; and the iron salt is at least one of ferric chloride, ferric nitrate, ferric citrate, and ferric nitrate nonahydrate.

[0015] In one embodiment of this application, the preparation conditions in step (1) are selected from at least one of (I) to (III):

[0016] (I) The biomass is at least one of rice straw, wheat straw, and corn cob;

[0017] (II) The activator is potassium carbonate and / or potassium hydroxide;

[0018] (III) The mass ratio of biomass to activator is 1:(0.5~2).

[0019] In one embodiment of this application, step (1) pre-treating the biomass includes: washing, drying, crushing, and sieving the biomass to obtain pre-treated biomass.

[0020] In one embodiment of this application, step (1) of preparing activated carbon includes: mixing pretreated biomass with an activator, activating it at 600-800°C for 100-150 min under an inert gas atmosphere, cooling, acid washing, water washing, filtering, and drying to obtain activated carbon.

[0021] In one embodiment of this application, the reaction conditions in step (2) are selected from at least one of (Ⅳ) to (Ⅶ):

[0022] (Ⅳ) The mass ratio of activated carbon, nitrogen source, and iron salt is (1~1.5):(20~25):(2.5~4);

[0023] (V) Calcination and carbonization are carried out by heating to 700-1000℃ at a heating rate of 3-10℃ / min;

[0024] (VI) The calcination time is 100-150 min;

[0025] (VII) The cooling condition is to cool to room temperature at a cooling rate of 1 to 5 °C / min.

[0026] The second aspect of the present invention provides a biomass-based antiviral agent prepared by the method described above.

[0027] The third aspect of this invention provides the application of a biomass-based antiviral agent as a catalyst in the inactivation of aquatic plant viruses. The biomass-based antiviral agent is as described above, and the aquatic plant virus is at least one of tobacco mosaic virus, pepper mild mottle virus, and cucumber mosaic virus. The biomass-based antiviral agent inactivates the aquatic plant virus by catalyzing the activation of persulfate to generate singlet oxygen.

[0028] In one embodiment of this application, the persulfate is sodium persulfate and / or potassium persulfate.

[0029] The fourth aspect of the present invention provides a method for inactivating aquatic plant viruses, the method comprising mixing a biomass-based antiviral agent, persulfate, and water containing aquatic plant viruses and carrying out a catalytic reaction to inactivate the aquatic plant viruses; the biomass-based antiviral agent is the biomass-based antiviral agent as described above.

[0030] In one embodiment of this application, the reaction conditions of the aquatic plant virus inactivation method are selected from at least one of (VIII) to (X):

[0031] (VIII) The mass ratio of biomass-based antiviral agent to persulfate is 1:(1-5);

[0032] (IX) The catalytic reaction was carried out at room temperature with a stirring speed of 100–300 r / min;

[0033] (X) The catalytic reaction time is 10–60 min.

[0034] The beneficial technical effects of this invention are as follows:

[0035] (1) This application first uses activators such as potassium hydroxide to react with biomass to increase the specific surface area of ​​carbon materials and improve electrical conductivity; secondly, it synthesizes biomass-based antiviral agents through one-step carbonization and calcination. Compared with the two-step calcination synthesis method, the preparation method of this application is simpler, more efficient and more economical.

[0036] (2) This application uses biomass such as corn cobs as raw materials, which has advantages such as wide availability, low price and green environmental protection. While realizing the resource utilization of biomass waste, it can also effectively reduce environmental pollution, which has certain economic and environmental significance.

[0037] (3) The biomass-based antiviral agent prepared in this application has a large specific surface area and a large number of active sites, and has high activity. It also has strong magnetism, which makes it easy to collect and utilize, and the preparation efficiency and production capacity are higher, which is conducive to industrial-scale production.

[0038] (4) The biomass-based antiviral agent prepared in this application is used as a catalyst in the inactivation of aquatic plant viruses. It inactivates aquatic plant viruses by catalyzing the activation of persulfate to generate singlet oxygen. It has a high inactivation rate and fast inactivation speed for viruses such as pepper mild mottle. It provides a low-cost, recyclable and efficient biomass-based antiviral agent catalyzing persulfate inactivation method for the inactivation of aquatic plant viruses, and has good prospects for promotion and application.

[0039] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0040] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0041] Figure 1 Scanning electron microscope (SEM) image and transmission electron microscope (TEM) image of the biomass-based antiviral agent prepared in Example 1 of this application;

[0042] Figure 2 This is a magnetic example diagram of the biomass-based antiviral agent prepared in Example 1 of this application;

[0043] Figure 3 Transmission electron microscopy (TEM) image and initial virus particle morphology image of the damage to pepper mild mottle virus particles by the biomass-based antiviral agent prepared in Example 4 of this application.

[0044] Figure 4 The graph shows the results of real-time fluorescence quantitative inactivation rate testing using Examples 5 and Comparative Examples 1-3 as test subjects in this application. Detailed Implementation

[0045] Unless otherwise defined, all technical and / or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. It should be understood that certain features of the invention (described in the context of separate embodiments for clarity) may also be provided in combination in a single embodiment. Conversely, multiple features of the invention (described in the context of a single embodiment for brevity) may also be provided separately or in any suitable combination or, where appropriate, in any other described embodiment of the invention. Certain features described in the context of various embodiments will not be considered essential features of those embodiments unless the embodiment is inoperable without those elements. The invention is further illustrated below by specific examples; however, it should be noted that the specific process conditions and results described in the embodiments of the invention are merely illustrative and should not be construed as limiting the scope of protection of the invention. All equivalent changes or modifications made in accordance with the spirit and essence of the invention should be covered within the scope of protection of the invention.

[0046] This invention provides a method for preparing a biomass-based antiviral agent, the method comprising:

[0047] (1) Select at least one of rice straw, wheat straw and corn cob as biomass, wash the biomass multiple times with deionized water, dry it, crush it with a ball mill, and pass it through a 60-100 mesh sieve to obtain pretreated biomass.

[0048] Potassium carbonate and / or potassium hydroxide are selected as activators. Pretreated biomass and activators are mixed at a mass ratio of 1:(0.5-2) and placed in a tube furnace. Under an inert gas atmosphere, the temperature is increased to 600-800℃ at a heating rate of 3-10℃ / min, activated for 100-150min, and then cooled to room temperature at a cooling rate of 1-5℃ / min. The inert gas is nitrogen and / or argon.

[0049] The material is acid-washed with 0.1 mol / L hydrochloric acid or 0.1 mol / L sulfuric acid to remove metallic impurities. Then it is repeatedly washed with deionized water, filtered, and dried to obtain activated carbon.

[0050] (2) Select dicyandiamide and / or melamine as nitrogen source, and select at least one of ferric chloride, ferric nitrate, ferric citrate, and ferric nitrate nonahydrate as iron salt. Dissolve the nitrogen source and iron salt in a solvent to prepare a solution containing nitrogen source and iron salt. The solvent is methanol and / or ultrapure water.

[0051] In a solution containing a nitrogen source and an iron salt, the mass ratio of nitrogen source to iron salt is (5-10):1.

[0052] Activated carbon is mixed with a solution containing a nitrogen source and iron salt, wherein the mass ratio of activated carbon, nitrogen source and iron salt is (1-1.5):(20-25):(2.5-4).

[0053] The temperature is increased to 700-1000℃ at a heating rate of 3-10℃ / min for carbonization and calcination for 100-150 min, and then cooled to room temperature at a cooling rate of 1-5℃ / min.

[0054] The impurities are removed by acid washing with 0.1 mol / L hydrochloric acid or 0.1 mol / L sulfuric acid, followed by repeated washing with deionized water, filtration, and drying at 100–110°C to obtain the biomass-based antiviral agent.

[0055] This invention provides a biomass-based antiviral agent prepared by the above-mentioned method.

[0056] This invention provides the application of a biomass-based antiviral agent as a catalyst in the inactivation of aquatic plant viruses. The biomass-based antiviral agent is as described above, and the aquatic plant virus is at least one of tobacco mosaic virus, pepper mild mottle virus, and cucumber mosaic virus. The biomass-based antiviral agent inactivates the aquatic plant virus by catalyzing the activation of persulfate to generate singlet oxygen. The persulfate is sodium persulfate and / or potassium peroxymonosulfate.

[0057] This invention provides a method for inactivating aquatic plant viruses, the method comprising:

[0058] A biomass-based antiviral agent and persulfate in a mass ratio of 1:(1-5) were mixed with water containing aquatic plant viruses and subjected to a catalytic reaction for 10-60 minutes at room temperature with a stirring speed of 100-300 r / min to inactivate the aquatic plant viruses.

[0059] The present invention will be described in detail below through specific examples and embodiments. It should also be understood that the following embodiments are only for specific illustration of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-essential improvements and adjustments made by those skilled in the art based on the above description of the present invention are within the scope of protection of the present invention. The specific process parameters, etc., in the following examples are merely examples within a suitable range; that is, those skilled in the art can make appropriate selections within the appropriate range based on the description herein, and are not intended to be limited to the specific values ​​in the examples below.

[0060] Example 1

[0061] (1) Rice straw was selected as biomass. The rice straw was washed multiple times with deionized water, dried, crushed by ball mill, and passed through an 80-mesh sieve to obtain pretreated rice straw.

[0062] Pretreated rice straw and potassium hydroxide were mixed at a mass ratio of 1:1 and placed in a tube furnace. Under a N2 atmosphere, the mixture was heated to 600°C at a heating rate of 5°C / min, activated for 100 min, and then cooled to room temperature at a cooling rate of 3°C / min.

[0063] The activated carbon was obtained by acid washing with 0.1 mol / L sulfuric acid, repeated washing with deionized water, filtration, and drying.

[0064] (2) Melamine and ferric nitrate nonahydrate in a mass ratio of 8:1 were dissolved in ultrapure water to prepare a solution containing a nitrogen source and iron salt.

[0065] Activated carbon was selected, and the mass ratio of activated carbon, nitrogen source, and iron salt was 1:20:2.5. The activated carbon was mixed with a solution containing nitrogen source and iron salt, heated to 700℃ at a heating rate of 5℃ / min, calcined for 100min, and cooled to room temperature at a cooling rate of 3℃ / min.

[0066] The biomass-based antiviral agent was obtained by acid washing with 0.1 mol / L sulfuric acid, repeated washing with deionized water, filtration, and drying at 110°C.

[0067] Example 2

[0068] (1) Wheat straw was selected as biomass. The wheat straw was washed multiple times with deionized water, dried, crushed by ball mill, and passed through a 100-mesh sieve to obtain pretreated wheat straw.

[0069] Pretreated wheat straw and potassium carbonate were mixed at a mass ratio of 1:0.5 and placed in a tube furnace. Under a N2 atmosphere, the mixture was heated to 800°C at a heating rate of 5°C / min and activated for 120 min. Then, it was cooled to room temperature at a cooling rate of 3°C / min.

[0070] The activated carbon was obtained by acid washing with 0.1 mol / L sulfuric acid, repeated washing with deionized water, filtration, and drying.

[0071] (2) Melamine and ferric citrate in a mass ratio of 10:1 were dissolved in ultrapure water to prepare a solution containing a nitrogen source and iron salt.

[0072] Activated carbon was selected, and the mass ratio of activated carbon, nitrogen source, and iron salt was 1:25:2.5. The activated carbon was mixed with a solution containing nitrogen source and iron salt, heated to 800℃ at a heating rate of 6℃ / min, calcined for 120min, and cooled to room temperature at a cooling rate of 4℃ / min.

[0073] The biomass-based antiviral agent was obtained by acid washing with 0.1 mol / L sulfuric acid, repeated washing with deionized water, filtration, and drying at 110°C.

[0074] Example 3

[0075] (1) Corn cobs were selected as biomass. The corn cobs were washed with deionized water multiple times, dried, crushed by ball mill, and passed through a 100-mesh sieve to obtain pretreated corn cobs.

[0076] Pretreated corn cobs and potassium carbonate were mixed at a mass ratio of 1:2 and placed in a tube furnace. Under a N2 atmosphere, the mixture was heated to 1000°C at a heating rate of 8°C / min, activated for 150 min, and then cooled to room temperature at a cooling rate of 5°C / min.

[0077] The activated carbon was obtained by acid washing with 0.1 mol / L hydrochloric acid, repeated washing with deionized water, filtration, and drying.

[0078] (2) Dissolve dicyandiamide and ferric chloride in ultrapure water at a mass ratio of 7:1 to prepare a solution containing a nitrogen source and iron salt;

[0079] Activated carbon was selected, and the mass ratio of activated carbon, nitrogen source, and iron salt was 1:21:3. The activated carbon was mixed with a solution containing nitrogen source and iron salt, heated to 1000℃ at a heating rate of 8℃ / min, calcined for 120min, and cooled to room temperature at a cooling rate of 5℃ / min.

[0080] The biomass-based antiviral agent was obtained by acid washing with 0.1 mol / L hydrochloric acid, repeated washing with deionized water, filtration, and drying at 100°C.

[0081] Example 4

[0082] Take 50 mL containing 3.0 × 10 10 A water sample containing a copy number of chili pepper mild mottle virus (CMP) was treated with 30 mg of potassium peroxymonosulfate and 15 mg of the biomass-based antiviral agent prepared in Example 3. The mixture was subjected to catalytic inactivation at room temperature and a stirring speed of 100 rpm for 30 min. A certain amount of the reaction solution was taken at time points of 0, 1, 3, 5, 15, and 30 min and quenched in an equal volume of methanol. The reaction solution was then suspended droplets on a copper grid, and the morphology of the virus particles was observed using transmission electron microscopy. The results are shown below. Figure 3 As shown.

[0083] Example 5

[0084] Take 50 mL containing 3.0 × 10 10A solution containing *V. spp.* var. *chia* was inactivated for 30 min at room temperature and a stirring speed of 100 rpm by adding 30 mg potassium persulfate and 15 mg of the biomass-based antiviral agent prepared in Example 3. A certain amount of the reaction solution was taken at time points of 0, 1, 3, 5, 15, and 30 min and quenched in an equal volume of methanol. Total RNA was then extracted and reverse transcribed. Finally, the copy number of *V. spp.* var. *chia* was detected by real-time quantitative PCR. The results are shown below. Figure 4 As shown.

[0085] Example 6

[0086] Take 50 mL containing 3.0 × 10 10 Add 30 mg of potassium peroxymonosulfate and 10 mg of the biomass-based antiviral agent prepared in Example 2 to water containing a copy number of chili pepper mottle virus per liter, and carry out a catalytic inactivation reaction for 30 min at room temperature and a stirring speed of 100 r / min.

[0087] Example 7

[0088] Take 50 mL containing 3.0 × 10 10 Add 30 mg of sodium persulfate and 8 mg of the biomass-based antiviral agent prepared in Example 1 to water containing a copy number of chili pepper mottle virus per liter, and carry out a catalytic inactivation reaction for 30 min at room temperature and a stirring speed of 100 r / min.

[0089] Comparative Example 1

[0090] 50mL contains 3.0×10 10 The number of copies per liter of chili peppers in the water body was slightly mottled with the virus.

[0091] Comparative Example 2

[0092] Take 50 mL containing 3.0 × 10 10 Add 30 mg of potassium peroxymonosulfate to water containing chili pepper mottle virus at a copy number per liter, and carry out a catalytic inactivation reaction for 30 min at room temperature and a stirring speed of 100 r / min.

[0093] Comparative Example 3

[0094] Take 50 mL containing 3.0 × 10 10 A water sample containing 10 mg of the biomass-based antiviral agent prepared in Example 3 was subjected to a catalytic inactivation reaction at room temperature and a stirring speed of 100 r / min for 30 min, with a copy number of chili pepper mottle virus per liter.

[0095] Performance testing

[0096] Morphology of the biomass-based antiviral agent: The structure and morphology of the biomass-based antiviral agent prepared in Example 1 were analyzed using a FEI Talos F200S transmission electron microscope and a Zeiss / Auriga FIB scanning electron microscope at different magnification scales. Transmission electron microscopy analysis is as follows: Figure 1 As shown in (b), scanning electron microscopy analysis is as follows: Figure 1 As shown in (a).

[0097] Magnetic properties: The magnetic properties of the biomass-based antiviral agent prepared in Example 1 were tested using a magnet. The test results are as follows: Figure 2 As shown.

[0098] Effects of biomass-based antiviral agents on the morphology of pepper mild mottle virus particles: The morphology of the initial pepper mild mottle virus particles in Example 4 was analyzed using a FEI Talos F200S transmission electron microscope. The results are as follows: Figure 3 As shown in (b); the analysis of the destruction of pepper mild mottle virus particles by the biomass-based antiviral agent of Example 4 is as follows. Figure 3 As shown in (a).

[0099] Inactivation activity: Using Examples 5 and Comparative Examples 1-3 as test subjects, at time points of 0, 1, 3, 5, 15, and 30 min, a certain amount of reaction solution from Examples 5 and Comparative Examples 1-3 was added to an equal volume of methanol for quenching. Total RNA was then extracted for reverse transcription. Finally, real-time fluorescence quantitative PCR was performed to detect the copy number of pepper mild mottle virus to determine the inactivation activity. The results are as follows: Figure 4 As shown;

[0100] like Figure 1 As shown, by Figure 1 (a) It can be seen that irregular, dense, bamboo-like carbon nanotubes germinate vertically on the biochar sheet and are sparsely distributed on the biochar sheet. Figure 1 (b) It can be seen that the nano-iron is encapsulated in the biochar layer, indicating that its structure becomes more stable, thereby promoting the extension of its lifespan as a persulfate catalyst.

[0101] like Figure 2 As shown, Figure 2 (a) indicates that the biomass-based antiviral agent is uniformly distributed in the aqueous solution. Figure 2 (b) indicates that under the action of an external magnetic field, biomass-based antiviral agents can be effectively separated from aqueous solutions.

[0102] like Figure 3 As shown, Figure 3 (a) indicates that the virus particles of pepper mottle virus aggregated 30 minutes after inactivation, and there were multiple breaks on the virus particles. Figure 3 (b) indicates that the initial virus particles are rod-shaped, intact, and well-dispersed.

[0103] like Figure 4 As shown, Figure 4 C t The concentration of pepper mild mottle virus at minute t represents the concentration of pepper mild mottle virus at minute t, where t is 1, 3, 5, 15, or 30 minutes; C0 represents the initial concentration of pepper mild mottle virus, i.e., at minute 0. This real-time fluorescence quantitative inactivation rate graph illustrates the inactivation efficiency of pepper mild mottle virus in water. The 2log2 inactivation rate graph in this application... 10 This indicates a 99% inactivation rate, 3log 10 This indicates an inactivation rate of 99.9%, 4log 10 This indicates an inactivation rate of 99.99%. Furthermore, in the application of this biomass-based antiviral agent catalyzing persulfate for the inactivation of plant viruses in aquatic bodies, the inactivation rate against pepper mild mottle virus can reach 5.9 log [value missing] in 1 minute. 10 This indicates that the biomass-based antiviral agent catalyzed persulfate prepared in this application for the inactivation of aquatic plant viruses has the characteristics of high efficiency and high inactivation rate.

[0104] The inactivation rates of comparative examples 1–3 were all less than 3log. 10 The inactivation rate is far lower than that of the method applied in this application, and the biomass-based antiviral agent has no inactivation activity when it acts directly on pepper mild mottle virus. It needs to work together with persulfate to achieve a highly efficient inactivation effect on pepper mild mottle virus.

[0105] Furthermore, tobacco mosaic virus, cucumber mosaic virus and pepper mild mottle virus are similar and belong to the same pepper virus family. Therefore, the biomass-based antiviral agent catalyzed persulfate prepared in this application can also be used to inactivate tobacco mosaic virus, cucumber mosaic virus, and even other types of pepper viruses.

[0106] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. The application of a biomass-based antiviral agent as a catalyst in the inactivation of aquatic plant viruses, characterized in that, The biomass-based antiviral agent inactivates aquatic plant viruses by catalyzing the activation of persulfate to generate singlet oxygen. The aquatic plant viruses are at least one of tobacco mosaic virus, pepper mild mottle virus, and cucumber mosaic virus. The preparation method of the biomass-based antiviral agent includes: (1) The biomass is pretreated, and the pretreated biomass is mixed with an activator at a mass ratio of 1:(0.5~2). Under an inert gas atmosphere, the mixture is activated at 600~800℃ for 100~150 min, cooled, acid-washed, washed with water, filtered, and dried to obtain activated carbon. The biomass is at least one of rice straw, wheat straw, and corn cob, and the activator is potassium carbonate and / or potassium hydroxide. (2) The activated carbon is mixed with a solution containing a nitrogen source and an iron salt, and heated to 700-1000°C at a heating rate of 3-10°C / min for calcination and carbonization for 100-150 min. After calcination and carbonization, the carbon is cooled to room temperature at a cooling rate of 1-5°C / min, acid-washed, water-washed, filtered, and dried to obtain a biomass-based antiviral agent. The nitrogen source is dicyandiamide and / or melamine. The iron salt is at least one of ferric chloride, ferric nitrate, ferric citrate, and ferric nitrate nonahydrate. The mass ratio of the activated carbon, the nitrogen source, and the iron salt is (1-1.5):(20-25):(2.5-4).

2. The application according to claim 1, characterized in that, The step (1) of pre-treating biomass includes: washing, drying, crushing, and sieving the biomass to obtain pre-treated biomass.

3. The application according to claim 1, characterized in that, The persulfate is potassium persulfate.

4. A method for inactivating aquatic plant viruses, characterized in that, The method includes mixing a biomass-based antiviral agent, persulfate, and water containing aquatic plant viruses and carrying out a catalytic reaction to inactivate the aquatic plant viruses; the biomass-based antiviral agent is the biomass-based antiviral agent used in claim 1.

5. The method for inactivating aquatic plant viruses according to claim 4, characterized in that, The reaction conditions of the method are selected from at least one of (VIII) to (X): (VIII) The mass ratio of the biomass-based antiviral agent to the persulfate is 1:(1~5); (IX) The catalytic reaction is carried out at room temperature with a stirring speed of 100~300 r / min; (X) The catalytic reaction time is 10~60 min.

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