A sugar alcohol solvent and its use in the preparation of a product containing ginkgo biloba leaf extract

Abstract

The application provides a sugar alcohol solvent and its application in preparation of a product containing ginkgo leaf extract, and belongs to the technical field of food and health care products. The sugar alcohol solvent comprises xylitol, and the mass percentage of xylitol in the sugar alcohol solvent is 30-50%. The sugar alcohol solvent is safe and non-toxic, has simple preparation process and low cost, can be used as a solubilizing solvent for the ginkgo leaf extract, can improve the solubility of the ginkgo leaf extract without affecting the stability and biological activity of ginkgolides and ginkgo flavones in the ginkgo leaf extract, can be used as an intermediate purification solvent for preparation of ginkgolide monomers, is safe and non-toxic, and can be used as a new solvent for the ginkgo leaf extract to prepare a liquid oral ginkgo leaf extract, so that liquid health care is realized, and safe drug delivery is realized for diagnosis and treatment of cardiovascular and cerebrovascular diseases of special groups (such as diabetic patients).

Description

Technical Field

[0001] This invention belongs to the field of food and health product technology, and in particular relates to a sugar alcohol solvent and its application in the preparation of products containing ginkgo leaf extract. Background Technology

[0002] Ginkgo biloba L., commonly known as white fruit, is a deciduous tree belonging to the Ginkgoaceae family and the Ginkgo genus. It is dioecious and is often referred to as a "living fossil." Currently, over 160 compounds have been isolated from ginkgo leaf extracts, mainly including flavonoids, terpenoid lactones, carboxylic acids, polysaccharides, amino acids, anthocyanins, alkylphenols, and polyisoprene alcohols. Ginkgo is the most widely recognized natural health supplement in Europe and America. Reports of the efficacy of ginkgo preparations in treating Alzheimer's disease have garnered even more attention. Originating in my country, its medicinal use dates back to the Ming Dynasty. The components of ginkgo possess extremely high pharmacological activity, including antibacterial, lipid-regulating, anticoagulant, antioxidant, and asthma-treating effects. The standard extract of ginkgo leaves is EGb761, patented by Schwabe in Germany. Since the 1960s, scholars both domestically and internationally have conducted extensive research on the effective medicinal components and pharmacological effects of ginkgo leaf extracts. The 2020 edition of the Chinese Pharmacopoeia records its use for "blood stasis obstructing the collaterals, chest pain, hemiplegia due to stroke, cough and asthma due to lung deficiency, and hyperlipidemia." Flavonoids are the main components of Ginkgo biloba extract and are also the main compounds responsible for its antioxidant effects and free radical scavenging. More than 50 flavonoids have been identified so far. The 2020 edition of the Chinese Pharmacopoeia stipulates that the content of flavonoids in Ginkgo biloba extract should not be less than 24%. The Chinese Pharmacopoeia requires that the flavonoid content in Ginkgo biloba leaves should not be less than 0.25%, mainly found in Ginkgo biloba leaves and seed coats. Currently, eight flavonoid aglycones have been identified: quercetin, kaempferol, isorhamnetin, luteolin, apigenin, eugenol, myricetin, and genkwain. There are also 17 flavonoid glycosides. The Chinese Pharmacopoeia stipulates that the content of terpene lactones in EGb should not be less than 6%. Currently, two compounds, diterpene lactones and sesquiterpene lactones, have been isolated from Ginkgo biloba leaf extract, namely ginkgolides A, B, C, J, K, L, N, P, Q and bilobalide. In addition, ginkgolide M is only found in Ginkgo biloba seed coat.

[0003] Ginkgo biloba extract is an active class of compounds prepared by solvent extraction from ginkgo leaves, and is an important natural product. The 2020 edition of the Chinese Pharmacopoeia stipulates that medicinal ginkgo biloba extract, calculated on a dried basis, should contain no less than 24.0% total flavonol glycosides and no less than 6.0% terpene lactones. Currently, commonly used industrial extraction solvents include methanol, ethanol, water, and acetone; among them, ethanol-water solution is the most commonly used extraction agent, but it suffers from low extraction efficiency, high temperature (above 60℃), cumbersome process, significant organic solvent volatilization loss, and high equipment costs. Developing green and environmentally friendly low-eutectic solvents is beneficial for solving these problems. Purification is a crucial step in the extraction and processing of natural products. Currently, there are many purification methods for ginkgolides and ginkgo flavonoids, mainly including resin purification, metal complexation, and recrystallization, but most of these methods use toxic and explosive solvents such as ethanol, n-hexane, petroleum ether, and ethylene glycol. It is necessary to develop green, non-toxic, and biodegradable environmentally friendly solvents to promote the green and sustainable development of the ginkgo biloba extract industry.

[0004] Ginkgo biloba extract (GBE) possesses anti-inflammatory, antioxidant, and anti-tumor activities. It can dilate cardiovascular and cerebrovascular vessels and arteries, preventing hypertension, hyperlipidemia, and diabetes, and is particularly popular among the elderly. Various preparations made from GBE are widely used in pharmaceuticals, health products, food additives, functional beverages, and cosmetics. Because the key active components of GBE, flavonoids and terpenoid lactones, have very low solubility in water, commercially available GBE preparations are primarily in solid dosage forms such as capsules, tablets, granules, and dispersible tablets. Solid dosage forms often suffer from drawbacks such as slow onset of action, fixed formulations that cannot be adjusted according to symptoms, and unfriendly effects on patients with swallowing difficulties. To overcome the shortcomings of solid dosage forms, in recent years, research has also been conducted on liquid dosage forms such as oral solutions, injections, and liposomes. These liquid dosage forms use water as the main solvent and often rely on organic solvents and surfactants for solubilization, resulting in limited solubilization effects, low effective GBE concentrations, large dosage volumes, and poor taste. Therefore, there is an urgent need to develop new liquid dosage forms of GBE with high solubility, stability, and patient-friendly properties.

[0005] Eutectic solvents, also known as deep eutectic solvents, refer to eutectic mixtures formed by two or more solid substances in a certain proportion through hydrogen bonding. The melting points of eutectic solvents are all lower than those of their components. Traditional solvents such as methanol, ethanol, and petroleum ether, also known as petrochemical solvents, have disadvantages such as environmental pollution, high toxicity, non-renewability, and strict requirements for large-scale applications. Ionic solutions, as alternative solvents, have long attracted scholarly attention. While ionic liquids are pollution-free, their high viscosity makes recycling inconvenient. Eutectic solvents overcome these disadvantages, possessing advantages such as simple preparation, good stability, high chemical purity, low cost, biodegradability, and environmental friendliness. As a novel environmentally friendly green solvent, they can enhance the extraction effects of microwaves and ultrasound, offering significant advantages in the extraction and deep dissolution of traditional Chinese medicine, and hold great potential in replacing traditional and toxic solvents.

[0006] For Ginkgo biloba leaves, which have significant medical and development value, there is an urgent need to find a new, safe, non-toxic, and low-cost green eutectic solvent that can be used for the extraction, deep dissolution, and preparation of liquid pharmaceutical preparations of Ginkgo biloba leaf extracts. Summary of the Invention

[0007] In view of this, the present invention provides the application of sugar alcohol solvent in the preparation of products containing ginkgo leaf extract.

[0008] The first objective of this invention is to provide a sugar alcohol solvent that can effectively dissolve ginkgo leaf extract, thereby improving its solubility without affecting the stability and bioactivity of ginkgo lactones and ginkgo flavonoids in the extract.

[0009] A second objective of this invention is to provide the sugar alcohol solvent as an intermediate purification solvent for the preparation of ginkgolide monomers.

[0010] The third objective of this invention is to provide the sugar alcohol solvent as a novel solvent in the preparation of Ginkgo biloba extract liquid oral solution, thereby achieving liquid health care and enabling safe administration for the diagnosis and treatment of cardiovascular and cerebrovascular diseases in special groups (such as diabetic patients).

[0011] The present invention provides a sugar alcohol solvent, including xylitol, wherein the mass percentage of xylitol in the sugar alcohol solvent is 30% to 50%.

[0012] Preferably, it also includes one or more of sorbitol, eurythritol, arbutin and erythritol.

[0013] Preferably, the sugar alcohol solvent comprises the following components in weight percentage:

[0014] Sorbitol 13%–14%, eurythritol 32%–38%, aritol 2.5%–3.5% and xylitol 35%–44%.

[0015] Preferably, the sugar alcohol solvent is derived from liquid xylitol.

[0016] Preferably, the sugar alcohol solvent comprises the following components in weight percentage: xylitol 30-45% and erythritol 10-30%.

[0017] This invention provides the application of the sugar alcohol solvent in the extraction of flavonoids from ginkgo leaves, wherein ginkgo leaves are mixed with the sugar alcohol solvent and extracted at 75-85°C for 3-5 hours.

[0018] This invention provides the application of the sugar alcohol solvent in the preparation of ginkgolide monomers, wherein the sugar alcohol solvent is used as an intermediate purification solvent.

[0019] This invention provides a liquid oral solution of ginkgo leaf extract, prepared from raw materials comprising the following components:

[0020] Xylitol 34%–36%, erythritol 10%–30%, glycerin 0.5%–1.5%, ginkgo leaf extract 0.3%–0.4%, menthol 0.003%–0.004%, and the balance being water.

[0021] This invention provides a method for preparing the aforementioned Ginkgo biloba extract liquid oral solution, comprising the following steps:

[0022] 1) Mix xylitol, erythritol and water to obtain a sugar alcohol solvent;

[0023] 2) Mix the ginkgo leaf extract with the sugar alcohol solvent described in step 1), dissolve and filter at 50-80°C, and collect the filtrate;

[0024] 3) Mix the filtrate from step 2) with menthol, treat at 68-72°C for 8-12 minutes, and then add glycerin to obtain a liquid oral solution of ginkgo biloba extract.

[0025] Preferably, the dissolution process in step 2) is accompanied by stirring, and the dissolution time is 0.5 to 1.5 hours.

[0026] Compared with existing technologies, the present invention has the following beneficial effects: The present invention provides a sugar alcohol solvent and its application in the extraction of Ginkgo biloba leaf extract. The sugar alcohol solvent provided by the present invention is safe and non-toxic, has a simple preparation process, and is low in cost. It can be used as a solubilizing solvent for Ginkgo biloba leaf extract, improving its solubility without affecting the stability and bioactivity of ginkgolides and ginkgo flavonoids in the extract. The sugar alcohol solvent can also be used as an intermediate purification solvent for the subsequent preparation of ginkgolide monomers, and is safe and non-toxic. The sugar alcohol solvent can also serve as a new solvent for Ginkgo biloba leaf extract, used in the preparation of liquid oral solutions of Ginkgo biloba leaf extract, realizing liquid health care and enabling safe administration for the diagnosis and treatment of cardiovascular and cerebrovascular diseases in special groups (such as diabetic patients).

[0027] Furthermore, the antibacterial effect of the Ginkgo biloba extract liquid oral liquid prepared using sugar alcohol solvent in this invention is superior to that of existing commercially available products, while the flavonoid content is comparable to that of commercially available products. Attached Figure Description

[0028] Figure 1 The chromatogram is of liquid xylitol derived from xylitol mother liquor;

[0029] Figure 2 The compounding process for preparing oral liquid of ginkgo leaf extract using sugar alcohol solvent;

[0030] Figure 3 This is a standard curve prepared using rutin as a standard.

[0031] Figure 4 Bacterial culture experiments were conducted on different oral liquids, including (a) a 35% xylitol solution; (b) the Ginkgo biloba extract liquid oral liquid prepared in Example 6; (c) the commercially available Greenbaokang oral liquid; and (d) the commercially available Nianniankang oral liquid. Detailed Implementation

[0032] The present invention provides a sugar alcohol solvent, including xylitol, wherein the mass percentage of xylitol in the sugar alcohol solvent is 30% to 50%.

[0033] In this invention, the xylitol content in the sugar alcohol solvent is preferably 32% to 48%, more preferably 35% to 45%.

[0034] In this invention, the sugar alcohol solvent is preferably derived from liquid xylitol; the liquid xylitol is a liquid mixture composed of xylitol, sorbitol, and other monosaccharide alcohols; more preferably, it is liquid xylitol obtained by extracting xylitol and sweet alcohol from xylose and xylitol byproducts (QB / T4574-2013); the liquid xylitol does not crystallize at room temperature and low temperature, and is a stable liquid that can be used as a novel low eutectic point solvent. The liquid xylitol of this invention possesses the physicochemical properties of general alcohol mixtures and has wide applications in industry.

[0035] In this invention, the liquid xylitol also includes sorbitol, euonymus alcohol, and aritol.

[0036] In this invention, the sugar alcohol solvent derived from liquid xylitol preferably comprises the following components in weight percentage:

[0037] Sorbitol 13%–14%, eurythritol 32%–38%, aritol 2.5%–3.5% and xylitol 35%–44%;

[0038] Further preferred components include the following components in weight percentage:

[0039] Sorbitol 13.8%, Euonymus 36%, Arlitol 3% and Xylitol 42%;

[0040] Furthermore, the sugar alcohol solvent also includes 6% of other ingredients.

[0041] In this invention, the preferred method for preparing the sugar alcohol solvent derived from liquid xylitol is as follows:

[0042] Liquid xylitol is heated at 65–75°C until all the xylitol is dissolved, then placed at 20–30°C for 10–14 hours for a first filtration, and the filtrate is collected. The filtrate is then allowed to stand at -14–-16°C for 10–14 hours for a second filtration, and the collected filtrate is the xylitol solvent. In this invention, the first or second filtration is preferably performed using a filter membrane, and the pore size of the filter membrane is preferably 0.22 μm.

[0043] In this invention, the heating temperature is preferably 68–72°C, more preferably 70°C; the placement temperature is preferably 24–26°C, more preferably 25°C; and the placement time is preferably 11–13 hours, more preferably 12 hours. The settling temperature is preferably -15°C, and the settling time is preferably 11–13 hours, more preferably 12 hours.

[0044] In this invention, the sugar alcohol solvent preferably comprises the following components in weight percentage: xylitol 30-45%, erythritol 10-30%; more preferably, it comprises xylitol 30-45%, erythritol 10-30%, and the balance being water; even more preferably, it comprises xylitol 33-38%, erythritol 14-24%, and the balance being water. In this invention, the above-mentioned sugar alcohol solvent is preferably prepared by using xylitol and erythritol standards.

[0045] The present invention also provides the application of the sugar alcohol solvent in the extraction of flavonoids from ginkgo leaves, wherein ginkgo leaves are mixed with the sugar alcohol solvent and extracted at 75-85°C for 3-5 hours.

[0046] In this invention, ginkgo leaves are mixed with the sugar alcohol solvent and extracted; the ginkgo leaves are preferably pulverized before being mixed with the sugar alcohol solution, and the pulverization mesh size is preferably 40 mesh; the mixing ratio of ginkgo leaves and sugar alcohol solution is preferably 1g:(5-15)ml, more preferably 1g:(6-9)ml, and even more preferably 1g:7ml; in this invention, the extraction temperature is preferably 78-82℃, more preferably 80℃, and the extraction time is preferably 3.5-4.5h, more preferably 4h.

[0047] This invention also provides the application of the sugar alcohol solvent in the preparation of ginkgolide monomers, wherein the sugar alcohol solvent serves as an intermediate purification solvent. This invention does not specifically limit the application of the sugar alcohol solvent; it is sufficient to use it as an intermediate purification solvent.

[0048] This invention provides a liquid oral solution of ginkgo leaf extract, prepared from raw materials comprising the following components:

[0049] Xylitol 34%–36%, erythritol 10%–30%, glycerin 0.5%–1.5%, ginkgo biloba extract 0.3%–0.4%, menthol 0.003%–0.004%, and the balance being water;

[0050] Preferably, it is prepared from raw materials comprising the following components: 35% xylitol, 14%–24% erythritol, 1.0% glycerol, 0.4% ginkgo leaf extract, 0.0035% menthol, and the balance being water.

[0051] This invention provides a method for preparing the aforementioned Ginkgo biloba extract liquid oral solution, comprising the following steps:

[0052] 1) Mix xylitol, erythritol and water to obtain a sugar alcohol solvent;

[0053] 2) Mix the ginkgo leaf extract with the sugar alcohol solvent described in step 1), dissolve and filter at 50-80°C, and collect the filtrate;

[0054] 3) Mix the filtrate from step 2) with menthol and treat at 68-72°C for 8-12 minutes to obtain a liquid oral solution of ginkgo biloba extract.

[0055] In this invention, the dissolution temperature is preferably 50-80°C, more preferably 65-75°C, and even more preferably 70°C; the dissolution time is preferably 0.5-1.5 h, more preferably 1 h; the filtration is preferably carried out using a filter membrane with a pore size of 0.22 μm.

[0056] In this invention, the filtrate is mixed with menthol and then preferably treated at 69-71°C for 9-11 minutes, and more preferably at 70°C for 10 minutes.

[0057] The Ginkgo biloba extract liquid oral solution prepared by this invention has good antibacterial properties and its flavonoid content is comparable to that of commercially available products.

[0058] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0059] Example 1

[0060] Liquid xylitol (QB / T4574-2013) is the xylitol mother liquor produced by xylitol production plants. It is the remaining product after extracting xylitol and other sweet alcohols. It is inexpensive and is purchased from xylitol production companies.

[0061] The preparation method of sugar alcohol solvent is as follows:

[0062] Liquid xylitol was heated at 70°C until all the xylitol was dissolved, allowed to stand at 25°C for 12 hours, and then filtered through a 0.22 μm filter membrane. The filtrate was allowed to stand at -15°C for 12 hours and then filtered again through a 0.22 μm filter membrane to obtain the xylitol solvent.

[0063] The components in the prepared sugar alcohol solvent were detected by liquid chromatography, and the results are as follows: Figure 1 As shown in Table 1, the specific components and contents of the sugar alcohol solvent were obtained through further calculations.

[0064] Table 1. Sugar alcohol solvent component content

[0065]

[0066] The refractive index of the prepared sugar alcohol solvent (treated liquid xylitol) meets the requirement of 60.

[0067] Example 2

[0068] The flavonoids in Ginkgo biloba leaves were extracted using the sugar alcohol solvent prepared in Example 1.

[0069] Materials: Ginkgo leaf powder, sugar alcohol solvent prepared in Example 1, ethanol, purified water

[0070] method:

[0071] ① Ginkgo leaves were pulverized to 40 mesh, and 500g was weighed and added to a round-bottom flask. Then, 3500mL of the sugar alcohol solvent prepared in Example 1 was added, and the water bath temperature was controlled at 80℃ for 4 hours. After 4 hours, the mixture was filtered through a 0.22μm filter membrane.

[0072] The filtrate was diluted with three times its volume of water and then the total flavonoid content in the filtrate was determined by ultraviolet spectrophotometry.

[0073] ② Set up a control experiment to compare the effect of sugar alcohol solvent on the dissolution effect of sugar alcohol solvent on Ginkgo biloba extract using conventional ethanol extraction method.

[0074] Weigh 500g of Ginkgo biloba leaf powder into a round-bottom flask, add 3500mL of 60% ethanol aqueous solution, and reflux extract twice, heating to reflux for 2 hours each time, controlling the water bath temperature at 65℃. After reflux, filter, combine the extracts, and measure the flavonoid content in the filtrate by ultraviolet spectrophotometry.

[0075] Results and Analysis

[0076] The ultraviolet spectrophotometer method was selected for flavonoid content testing.

[0077] Determination of standard curve

[0078] Preparation of standard solution: Accurately weigh 23 mg of rutin and place it in a 100 mL volumetric flask. Dissolve and dilute to volume with a 30% ethanol solution.

[0079] Preparation of the standard curve: Accurately pipette 0.5, 1, 2, 3, and 4 mL of the above rutin standard solution into 10 mL volumetric flasks. Add 5 mL of 30% ethanol aqueous solution and 0.3 mL of 5% NaNO2 solution, shake well, and let stand for 5 min. Then add 0.3 mL of 10% Al(NO3)3 solution. After 6 min, add 2 mL of 1 mol / L NaOH solution, shake well, and dilute to volume with 30% ethanol aqueous solution. Measure at 510 nm after 10 min. Determine the relationship between absorbance A and concentration C, and calculate the regression equation using the least squares method.

[0080] The flavonoid content in the extract was measured using the above methods, and a rutin standard curve was plotted. The results are as follows. Figure 3 As shown. The experimental data are as follows:

[0081] Table 2 Absorbance of different concentrations of rutin

[0082]

[0083] The regression equation was calculated using the least squares method: A = 1.32513C + 0.00133, R0 2 =0.99773.

[0084] Sample determination

[0085] Sample determination: Accurately transfer 10 mL of the test solution into a 50 mL volumetric flask using a pipette. Add 10 mL of ethanol, 1 mL of 5% NaNO2 solution, 1 mL of 10% Al(NO3)3 solution, and 5 mL of 1 mol / L NaOH solution respectively according to the above method. Then, make up to volume with 30% ethanol, let stand for 10 min, and then measure. Calculate the total flavonoid content using the regression equation method.

[0086] For the ethanol-extracted sample, three parallel absorbance measurements were performed, yielding values ​​of 1.062, 1.065, and 1.065, respectively. The average value was used to determine the concentration, which was 0.8019 mg / ml. The calculated flavonoid extraction rate was 72.9%. For the sugar alcohol solvent extract, three parallel absorbance measurements were performed, yielding values ​​of 0.525, 0.527, and 0.529, respectively. The average value was used to determine the concentration, which was 0.3967 mg / ml. The calculated flavonoid extraction rate was 81.1%.

[0087] calculate:

[0088] ①The total flavonoid content of commercially available ginkgo leaf extract is 24%, and the total flavonoid content of 500g ginkgo leaf powder is 120g. After dilution of the ethanol-water extract filtrate (in order to meet the concentration range of the standard curve), the total volume is 21800ml (actual measurement after filtration), and the concentration is 5.5mg / ml (120000mg÷21800ml).

[0089] Therefore, the extraction rate is [0.8019 mg / ml (actual measured concentration) ÷ (5.5 mg / ml ÷ 5 (5 times dilution, 10 ml to 50 ml volumetric flask, refer to sample determination method))] × 100% = 72.9%.

[0090] ②The total flavonoid content of commercially available ginkgo leaf extract is 24%, and the total flavonoid content of 500g ginkgo leaf powder is 120g. After dilution of the extract filtrate with sugar alcohol solvent (in order to meet the concentration range of the standard curve), the total volume is 21800ml (actual measurement after filtration), and the concentration is 2.45mg / ml (120000mg÷49000ml).

[0091] Therefore, the extraction rate is [0.3967 mg / ml (actual measured concentration) ÷ (2.45 mg / ml ÷ 5 (5 times dilution, 10 ml to 50 ml volumetric flask, refer to sample determination method))] × 100% = 81.1%.

[0092] It can be seen that the flavonoid extraction rate of ethanol aqueous solution is 72.9%, while the flavonoid extraction rate of sugar alcohol solvent is improved to 81.1%.

[0093] Example 3

[0094] A sugar alcohol solvent with the following composition: xylitol 35 wt%, erythritol 14 wt%, and water 51 wt%.

[0095] Example 4

[0096] A sugar alcohol solvent with the following composition: xylitol 35 wt%, erythritol 19 wt%, and water 46 wt%.

[0097] Example 5

[0098] A sugar alcohol solvent with the following composition: xylitol 35 wt%, erythritol 24 wt%, and water 41 wt%.

[0099] Example 6

[0100] To prepare 140g of Ginkgo biloba extract oral liquid: accurately weigh 49g of xylitol, 19.6g of erythritol, 1.4g of glycerin, 0.56g of Ginkgo biloba extract, 4.9mg of menthol, and the remainder water.

[0101] Preparation method: Xylitol, erythritol and water are mixed to obtain a sugar alcohol solvent; Ginkgo biloba extract is mixed with the sugar alcohol solvent, dissolved at 50°C for 1 hour, filtered and the filtrate is collected; the filtrate is mixed with menthol and treated at 70°C for 10 minutes to obtain Ginkgo biloba extract for oral administration.

[0102] Example 7

[0103] To prepare 140g of Ginkgo biloba extract oral liquid: accurately weigh 49g of xylitol, 26.6g of erythritol, 1.4g of glycerin, 0.56g of Ginkgo biloba extract, 4.9mg of menthol, and the remainder water.

[0104] Preparation method: Xylitol, erythritol and water are mixed to obtain a sugar alcohol solvent; Ginkgo biloba extract is mixed with the sugar alcohol solvent, dissolved at 80°C for 1 hour, filtered and the filtrate is collected; the filtrate is mixed with menthol and treated at 70°C for 10 minutes to obtain Ginkgo biloba extract for oral administration.

[0105] Example 8

[0106] To prepare 140g of Ginkgo biloba extract oral liquid: accurately weigh 49g of xylitol, 33.6g of erythritol, 1.4g of glycerin, 0.56g of Ginkgo biloba extract, 4.9mg of menthol, and the remainder water.

[0107] Preparation method: Xylitol, erythritol and water are mixed to obtain a sugar alcohol solvent; Ginkgo biloba extract is mixed with the sugar alcohol solvent, dissolved at 70°C for 1 hour, filtered and the filtrate is collected; the filtrate is mixed with menthol and treated at 70°C for 10 minutes to obtain Ginkgo biloba extract for oral administration.

[0108] Experimental Example 1

[0109] Antibacterial activity test: The liquid oral solution of Ginkgo biloba extract prepared in Example 6 was compared with commercially available oral solutions of Ginkgo biloba extract. The solutions were placed in sterile petri dishes containing culture medium and incubated in a constant temperature incubator for 48 hours before observation. It was found that both commercially available oral solutions exhibited relatively obvious bacterial growth, such as (…). Figure 4As shown in the figure, although the Ginkgo biloba extract liquid oral solution prepared in Example 6 also contained some bacteria, the number of bacteria was relatively small. Furthermore, in the control group using sugar alcohol solvent, no bacterial growth was observed, indicating that xylitol in the formulation has a certain antibacterial effect.

[0110] Flavonoid content determination: The method for determining flavonoid content is the same as the sample determination method described in Example 2.

[0111] Table 3. Measurement results of the three products

[0112]

[0113] It is evident that the Ginkgo biloba extract liquid oral liquid prepared by this invention has good antibacterial properties, and its flavonoid content is comparable to that of commercially available products.

[0114] As can be seen from the above embodiments, the flavonoid extraction rate of the sugar alcohol solvent provided by the present invention is higher, and the antibacterial effect of the prepared Ginkgo biloba extract liquid oral liquid is better than that of existing commercial products, while the flavonoid content is comparable to that of commercial products.

[0115] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A sugar alcohol solvent, characterized in that, The components include the following components by mass percentage: Sorbitol 13%~14%, eurythritol 32%~38%, aritol 2.5%~3.5% and xylitol 35%~44%; The sugar alcohol solvent is derived from liquid xylitol, and the preparation method is as follows: Liquid xylitol is heated at 65-75°C until all the xylitol is dissolved, and then placed at 20-30°C for 10-14 hours. The mixture is then filtered for the first time, and the filtrate is collected. The filtrate is then allowed to stand at -14-16°C for 10-14 hours, and then filtered for the second time. The collected filtrate is the xylitol solvent.

2. The application of the sugar alcohol solvent according to claim 1 in the extraction of Ginkgo biloba flavonoids, characterized in that, Ginkgo leaves were mixed with the sugar alcohol solvent and extracted at 75-85°C for 3-5 hours.

3. The application of the sugar alcohol solvent according to claim 1 in the preparation of ginkgolide monomers, characterized in that, The sugar alcohol solvent is used as an intermediate purification solvent.

4. A liquid oral solution of ginkgo leaf extract, characterized in that, It is prepared from raw materials including the following components: Xylitol 34%~36%, erythritol 10%~30%, glycerin 0.5%~1.5%, ginkgo leaf extract 0.3%~0.4%, menthol 0.003%~0.004%, and the balance being water.

5. The method for preparing the liquid oral solution of Ginkgo biloba extract according to claim 4, characterized in that, Includes the following steps: 1) Xylitol, erythritol and water are mixed to obtain a sugar alcohol solvent; 2) Mix the ginkgo leaf extract with the sugar alcohol solvent described in step 1), dissolve and filter at 50-80°C, and collect the filtrate; 3) Mix the filtrate obtained in step 2) with menthol, treat at 68~72℃ for 8~12 min, and then add glycerin to obtain a liquid oral solution of ginkgo biloba extract.

6. The preparation method according to claim 5, characterized in that, The dissolution process described in step 2) is accompanied by stirring, and the dissolution time is 0.5~1.5h.

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