Method for decoloring livestock and poultry breeding wastewater

Abstract

The application relates to the technical field of wastewater treatment, and particularly discloses a livestock and poultry breeding wastewater decoloring method. The livestock and poultry breeding wastewater decoloring method provided by the application comprises the following steps: pretreatment, anaerobic treatment, flocculation and sedimentation. The specific steps of the anaerobic treatment are as follows: adding an anaerobic bacterial agent into the pretreated livestock and poultry breeding wastewater, and performing anaerobic treatment for 24-48 hours. The anaerobic bacterial agent comprises fluorescent pseudomonas, clostridium butyricum, bifidobacterium longum and lactic acid bacteria. The viable bacterial count of the anaerobic bacterial agent is greater than or equal to 500 billion CFU / g. The livestock and poultry breeding wastewater decoloring method provided by the application can remove suspended particulate matters in the livestock and poultry breeding wastewater, reduce the ammonia nitrogen content, and efficiently degrade organic matters, so that the purpose of rapid decoloring is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of wastewater treatment, in particular to a decolorization method of livestock and poultry breeding wastewater. BACKGROUND

[0002] With the development of animal husbandry, livestock and poultry breeding wastewater pollution has attracted more and more attention. Livestock and poultry breeding wastewater includes breeding farm washing water, livestock and poultry urine wastewater, breeding farm residual manure wastewater, and feed residue wastewater. Livestock and poultry breeding wastewater has the characteristics of high organic matter concentration (COD), high ammonia nitrogen (NH3-N) content, high suspended particulate matter (SS) concentration, and high colority. Therefore, if livestock and poultry breeding wastewater is not effectively treated, it may cause a series of infectious diseases, and may hinder water self-purification, causing permanent water pollution and seriously affecting the surrounding environment.

[0003] In recent years, most pig farms use iron salt flocculants (such as FeCl3, polymeric ferric sulfate, polyaluminum chloride-polyacrylamide, etc.) for livestock and poultry breeding wastewater treatment. The results show that iron salt flocculants have significant effect on the removal of suspended particulate matter SS, but the removal effect of organic pollutants and pathogenic microorganisms in livestock and poultry breeding wastewater is not obvious, resulting in poor decolorization effect of wastewater. This problem has become a big problem for the current breeding industry personnel.

[0004] Therefore, there is an urgent need to provide a decolorization method of livestock and poultry breeding wastewater to solve the current problems faced by the breeding industry. SUMMARY

[0005] In view of the current decolorization difficulty of livestock and poultry breeding wastewater in the breeding industry, the present application provides a decolorization method of livestock and poultry breeding wastewater.

[0006] The decolorization method of livestock and poultry breeding wastewater provided by the present application adopts the following technical scheme:

[0007] A decolorization method of livestock and poultry breeding wastewater, comprising the following steps: pretreatment, anaerobic treatment, flocculation, and sedimentation treatment.

[0008] Anaerobic treatment: adding an anaerobic bacterial agent to the pretreated livestock and poultry breeding wastewater, and anaerobic treatment for 24-48h.

[0009] The anaerobic bacterial agent comprises Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum, and lactic acid bacteria; and the viable bacterial count of the anaerobic bacterial agent is ≥50 billion CFU / g.

[0010] The application provides a livestock and poultry breeding wastewater decolorization method, which adopts an anaerobic bacterial agent to treat livestock and poultry breeding wastewater, uses biological enzymes generated by microorganisms in the anaerobic bacterial agent to oxidize or reduce colored molecules, destroys unsaturated groups and colored group sequences of the colored molecules, hydrolyzes insoluble organic matters in the livestock and poultry breeding wastewater into soluble organic matters, and converts macromolecular organic matters that are difficult to biodegrade into small molecular substances that are easy to biodegrade, so that the decolorization purpose is achieved. In addition, the microorganisms in the anaerobic bacterial agent can use the small molecular organic matters decomposed as energy to promote the growth and reproduction of the microorganisms, so that the wastewater decolorization process is accelerated.

[0011] The anaerobic bacterial agent of the application comprises Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria. The above anaerobic bacterial agent is added to livestock and poultry breeding wastewater, extracellular enzymes generated by microorganisms can hydrolyze macromolecular organic matters into small molecules, which are then absorbed by Pseudomonas fluorescens and Bifidobacterium longum, so as to be converted into simpler compounds (such as alcohols, lactic acid, volatile fatty acids, carbon dioxide, hydrogen and the like) and secreted to the extracellular. These simple compounds are further converted into acetic acid, hydrogen and the like by Clostridium butyricum and lactic acid bacteria and the like, so that the purpose of wastewater purification is achieved. Compared with chemical decolorization in the related art, the biological decolorization method provided by the application has the advantages of short decolorization time, low treatment cost and no secondary pollution, and therefore has a good application prospect.

[0012] Optionally, the anaerobic bacterial agent comprises the following components in parts by weight: 1-2 parts of Pseudomonas fluorescens, 0.3-0.8 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium longum and 2-5 parts of lactic acid bacteria.

[0013] In some embodiments, the Pseudomonas fluorescens can be in a weight fraction of 1-2 parts or 2-3 parts.

[0014] In a specific embodiment, the Pseudomonas fluorescens can be in a weight fraction of 1 part, 2 parts or 3 parts.

[0015] In some embodiments, the Clostridium butyricum can be in a weight fraction of 0.3-0.5 parts or 0.5-0.8 parts.

[0016] In a specific embodiment, the Clostridium butyricum can be in a weight fraction of 0.3 parts, 0.5 parts or 0.8 parts.

[0017] In some embodiments, the Bifidobacterium longum can be in a weight fraction of 1-1.5 parts or 1.5-2 parts.

[0018] In a specific embodiment, the Bifidobacterium longum can be in a weight fraction of 1 part, 1.5 parts or 2 parts.

[0019] In some embodiments, the weight parts of the lactic acid bacteria can be 2-3.5 parts or 3.5-5 parts.

[0020] In a specific embodiment, the weight parts of the lactic acid bacteria can also be 2 parts, 3.5 parts or 5 parts.

[0021] Optionally, the added amount of the anaerobic bacteria agent is 50-150 g / m 3 .

[0022] In some embodiments, the added amount of the anaerobic bacteria agent can be 30-50 g / m 3 , 30-100 g / m 3 , 30-150 g / m 3 , 30-180 g / m 3 , 50-100 g / m 3 , 50-150 g / m 3 , 50-180 g / m 3 , 100-150 g / m 3 , 100-180 g / m 3 or 150-180 g / m 3 .

[0023] In a specific embodiment, the added amount of the anaerobic bacteria agent can be 30 g / m 3 , 50 g / m 3 , 100 g / m 3 , 150 g / m 3 or 180 g / m 3 .

[0024] Optionally, the activator is selected from one or more of ethylenediamine phosphate, propylenediamine phosphate, dimethylbenzene, sodium fluorosilicate, ammonium sulfate, ammonium chloride, ferrous sulfate and ammonium hydroxide.

[0025] Optionally, the pretreatment adopts three-stage filtration, and the pore sizes of the filters of the three-stage filtration are 3-4 mm, 2-3 mm and 1-2 mm in sequence.

[0026] Optionally, the flocculation step adopts a flocculant, and the flocculant is selected from one or more of polyaluminum chloride, polyferric sulfate, aluminum sulfate and anionic polyacrylamide.

[0027] By adding the flocculant to the wastewater after anaerobic treatment, the present application can coagulate the colloidal particles in the livestock and poultry breeding wastewater together to form water-insoluble flocculation aggregates through the adsorption, bridging and crosslinking of the flocculant, so as to achieve the purpose of further decolorization and removal of solid suspensions through precipitation.

[0028] Optionally, the precipitation treatment adopts inclined plate precipitation, and the time is 1-3h.

[0029] The precipitation of the application adopts inclined plate precipitation, which helps to increase the precipitation area, improve the sinking speed of the flocculation aggregates, improve the removal rate of the flocculation aggregates, and shorten the precipitation time.

[0030] Optionally, the livestock and poultry breeding wastewater after the precipitation treatment is filtered by using a microporous filter membrane with a pore size of 0.45μm.

[0031] In summary, the application has the following beneficial effects:

[0032] 1. In the decolorization method of the livestock and poultry breeding wastewater provided by the application, anaerobic bacteria agents are used for anaerobic treatment of the livestock and poultry breeding wastewater, and the organic matter in the livestock and poultry breeding wastewater is converted into stable and harmless substances by using the metabolism of microorganisms, so as to achieve the purpose of decolorization and purification.

[0033] 2. The anaerobic bacteria agents of the application include Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria, and the livestock and poultry breeding wastewater is treated by using the anaerobic bacteria agents, and is combined with flocculation, precipitation and other steps, so that the chemical oxygen demand COD value of the discharged water is only 102-134mg / L, the ammonia nitrogen concentration is only 50.2-64.7mg / L, both of which meet and are far lower than the discharge standard GB18595-2001, and the total suspended solids content SS of the discharged water is 125-155mg / L, and the colority is 11-22PCU.

[0034] 3. The application further controls the addition amount of the anaerobic bacteria agents in the range of 50-150g / m 3 , so that the treatment effect of the livestock and poultry breeding wastewater is better, the cost is lower, and the colority of the discharged water can be controlled to be below 20PCU. DETAILED DESCRIPTION

[0035] The application provides a decolorization method of livestock and poultry breeding wastewater, which comprises the following steps:

[0036] (1) Pretreatment: firstly, the livestock and poultry breeding wastewater is subjected to three-stage filtration to remove manure, solid particles, floating matter and the like in the livestock and poultry breeding wastewater in sequence; the pore sizes of the filters in the three-stage filtration are 3-4mm, 2-3mm and 1-2mm respectively.

[0037] (2) Anaerobic treatment: anaerobic bacteria agents are added to the livestock and poultry breeding wastewater after the pretreatment according to an addition amount of 50-150g / m 3 , and the anaerobic treatment is performed for 24-48h.

[0038] The anaerobic bacteria agent comprises the following components by weight: 1-2 parts of Pseudomonas fluorescens, 0.3-0.8 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium longum, 2-5 parts of lactic acid bacteria, and 5-10 parts of an activator. The activator is selected from one or more of ethylenediamine phosphate, propylenediamine phosphate, dimethylbenzene, sodium fluorosilicate, ammonium sulfate, ammonium chloride, ferrous sulfate, and ammonium hydroxide.

[0039] (3) Flocculation: a flocculant is added to the livestock breeding wastewater after anaerobic treatment, and is left to stand for 12-24 h. The amount of the flocculant added is 10-20 g / m 3 of the livestock breeding wastewater, calculated by volume. The flocculant is selected from one or more of polyaluminum chloride, polyferric sulfate, aluminum sulfate, and anionic polyacrylamide.

[0040] (3) Precipitation: the livestock breeding wastewater after flocculation treatment is sent to a inclined plate precipitation zone, and is left to stand for 1-3 h; then a microfiltration membrane with a pore size of 0.45 μm is used to filter the precipitated water, to obtain discharge water.

[0041] The anaerobic bacteria agent comprises Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum, and lactic acid bacteria; the viable bacterial count of the anaerobic bacteria agent is ≥ 500 billion CFU / g; the anaerobic bacteria agent comprises the following components by weight: 1-2 parts of Pseudomonas fluorescens, 0.3-0.8 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium longum, and 2-5 parts of lactic acid bacteria.

[0042] The strains used in the present application are all existing strains, and their sources are as follows: the Pseudomonas fluorescens has a preservation number of CGMCC No. 8525; the Clostridium butyricum has a preservation number of GDMCC NO: 60753; the Bifidobacterium longum has a preservation number of CGMCC NO. 15032; the lactic acid bacteria has a preservation number of CCTCC NO: M2017382; the remaining raw materials, reagents, solvents, etc. of the present application can be obtained by commercial purchase.

[0043] The present application is further described in detail below in combination with preparation examples, examples, and performance detection tests.

[0044] Preparation Examples 1-9

[0045] Preparation Examples 1-9 each provide an anaerobic bacteria agent.

[0046] The differences between the above preparation examples are the amounts of the components in the anaerobic bacteria agent, and are specifically shown in Table 1 below.

[0047] Preparation Examples 1-9 each provide an anaerobic bacteria agent.

[0048] (1) Activation and culture of strains: the test strains of Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria stored at -80°C were activated for 3 times and then transferred to nutrient liquid medium for culture, and the culture was counted by dilution plating when it reached the logarithmic phase;

[0049] (2) Preparation of each bacterial powder: each bacterial liquid was centrifuged by a centrifuge, and a protective agent was added to each bacterial liquid, and a bacterial powder was prepared by a spray dryer, and the bacterial content of the bacterial powder was adjusted to 100 billion cfu / g for standby; wherein the protective agent is a mixture of dextrin, anhydrous sodium sulfate and sodium metabisulfite in a mass ratio of 3:2:0.5, and the addition amount of the protective agent is 20% of the mass of the bacterial liquid;

[0050] (3) Preparation of anaerobic bacteria agent: 1.5 g of Pseudomonas fluorescens, 0.5 g of Clostridium butyricum, 1.5 g of Bifidobacterium longum, 3.5 g of lactic acid bacteria and 5 g of propylene diamine phosphate were mixed uniformly to obtain an anaerobic bacteria agent, which was stored at 4°C for standby.

[0051] Table 1 Addition amount of each component in the anaerobic bacteria agent provided in preparation examples 1-9

[0052]

[0053]

[0054] Comparative preparation example 1

[0055] Comparative preparation example 1 provides an anaerobic bacteria agent.

[0056] The difference between the above comparative preparation example and preparation example 2 is that the addition amount of Pseudomonas fluorescens is 0.

[0057] Comparative preparation example 2

[0058] Comparative preparation example 2 provides an anaerobic bacteria agent.

[0059] The difference between the above comparative preparation example and preparation example 2 is that the addition amount of Clostridium butyricum is 0.

[0060] Comparative preparation example 3

[0061] Comparative preparation example 3 provides an anaerobic bacteria agent.

[0062] The difference between the above comparative preparation example and preparation example 2 is that the addition amount of Bifidobacterium longum is 0.

[0063] Comparative preparation example 4

[0064] Comparative preparation example 4 provides an anaerobic bacteria agent.

[0065] The difference between the above comparative preparation example and preparation example 2 is that the addition amount of lactic acid bacteria is 0.

[0066] Comparative Preparation Example 5

[0067] Comparative Preparation Example 5 provides an anaerobic bacteria agent.

[0068] The above comparative preparation example differs from Preparation Example 2 in that the Pseudomonas fluorescens is replaced by a yeast, and the accession number of the yeast is CCTCC No: M2013725.

[0069] Comparative Preparation Example 6

[0070] Comparative Preparation Example 6 provides an anaerobic bacteria agent.

[0071] The above comparative preparation example differs from Preparation Example 2 in that the Pseudomonas fluorescens is replaced by a nitrite bacteria, and the accession number of the nitrite bacteria is CCTCC NO. M 2018030.

[0072] Comparative Preparation Example 7

[0073] Comparative Preparation Example 7 provides an anaerobic bacteria agent.

[0074] The above comparative preparation example differs from Preparation Example 2 in that the Clostridium butyricum is replaced by a nitrite bacteria, and the accession number of the nitrite bacteria is CCTCC NO. M 2018030.

[0075] Examples 1-9

[0076] Examples 1-9 respectively provide a method for decolorizing livestock breeding wastewater.

[0077] The above examples differ in that the anaerobic treatment in the method for decolorizing livestock breeding wastewater is respectively derived from Preparation Examples 1-9.

[0078] The method for decolorizing livestock breeding wastewater provided by Examples 1-9 comprises the following steps:

[0079] (1) Pretreatment: First, the livestock breeding wastewater is subjected to three-stage filtration to remove manure, solid particles, floating matter, etc. in the livestock breeding wastewater; the pore sizes of the filters in the three-stage filtration are 3 mm, 2 mm and 1 mm, respectively.

[0080] (2) Anaerobic treatment: 100 g / m 3 of the anaerobic bacteria agent is added to the pretreated livestock breeding wastewater, and the anaerobic treatment is performed for 48 h.

[0081] (3) Flocculation: Polymeric ferric sulfate is added to the livestock breeding wastewater after the anaerobic treatment, and the addition amount of the polymeric ferric sulfate is 15 g / m 3 , and the residence time is 12-24 h.

[0082] (4) Sedimentation: the livestock and poultry breeding wastewater after flocculation treatment was sent to the inclined plate sedimentation zone and stayed for 2 h. Then, the sedimentation water was filtered by using a microporous filter membrane with a pore size of 0.45 μm to obtain the discharge water.

[0083] Examples 10-13

[0084] Examples 10-13 respectively provide a livestock and poultry breeding wastewater decolorization method.

[0085] The above examples differ in the amount of anaerobic bacteria agent added, which is shown in Table 2 as follows.

[0086] Table 2 Amount of anaerobic bacteria agent added in Examples 2, 10-13

[0087] Examples Amount of anaerobic bacteria agent added (g / m 3 )]]> 2 100 10 30 11 50 12 150 13 180

[0088] Comparative Examples 1-7

[0089] Comparative Examples 1-7 respectively provide a livestock and poultry breeding wastewater decolorization method.

[0090] The above comparative examples and Example 2 differ in that the anaerobic treatment in the livestock and poultry breeding wastewater decolorization method is respectively derived from Comparative Preparation Examples 1-7.

[0091] Detection test

[0092] The indicators of the discharge water obtained from Examples 1-13 and Comparative Examples 1-7 were detected, and the results are shown in Table 3.

[0093] The COD detection method refers to GB11914 "Determination of Chemical Oxygen Demand in Water by Dichromate Method"; the ammonia nitrogen content of the discharge water was detected by using an ET6500 ammonia nitrogen concentration detector. The suspended particulate matter SS content of the discharge water was detected by using an ET9270 total suspended matter detector. The color of the discharge water was detected by using an ET7240 platinum cobalt colorimeter (0-500 PCU).

[0094] Table 3 Detection results of indicators of discharge water of Examples 1-13 and Comparative Examples 1-7

[0095]

[0096]

[0097] According to the detection results in Table 3, the anaerobic bacteria agent prepared by using Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria in the application examples 1-13 is used to treat the livestock breeding wastewater, and the chemical oxygen demand COD value of the discharged water is only 102-134 mg / L, and the ammonia nitrogen concentration is only 50.2-64.7 mg / L, both of which meet and are far lower than the discharge standard GB18595-2001, and the total suspended solids content SS of the discharged water is 125-155 mg / L, and the color is 11-22 PCU.

[0098] The anaerobic bacteria agent prepared by using any three of Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria in the comparative examples 1-4 is used to treat the livestock breeding wastewater, and the organic matter, solid suspended matter and color of the discharged water are higher; the chemical oxygen demand COD value is 143-178 mg / L, the ammonia nitrogen concentration is 96-127 mg / L, the total suspended solids content SS is 165-212 mg / L, and the color is 29-35 PCU.

[0099] The anaerobic bacteria agent prepared by using yeast, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria in the comparative example 5 is used to treat the livestock breeding wastewater, and the chemical oxygen demand COD value of the discharged water is 160 mg / L, the ammonia nitrogen concentration is 94 mg / L, the total suspended solids content SS is 168 mg / L, and the color is 29 PCU.

[0100] The anaerobic bacteria agent prepared by using any three of nitrite bacteria, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria in the comparative example 6 is used to treat the livestock breeding wastewater, and the chemical oxygen demand COD value of the discharged water is 156 mg / L, the ammonia nitrogen concentration is 46 mg / L, the total suspended solids content SS is 152 mg / L, and the color is 32 PCU.

[0101] The anaerobic bacteria agent prepared by using any three of Pseudomonas fluorescens, nitrite bacteria, Bifidobacterium longum and lactic acid bacteria in the comparative example 7 is used to treat the livestock breeding wastewater, and the chemical oxygen demand COD value of the discharged water is 148 mg / L, the ammonia nitrogen concentration is 37 mg / L, the total suspended solids content SS is 149 mg / L, and the color is 20 PCU.

[0102] In summary, in the decolorization method of livestock breeding wastewater provided by the application examples 1-13, the anaerobic bacteria agent prepared by using Pseudomonas fluorescens, Clostridium butyricum, Bifidobacterium longum and lactic acid bacteria is used to treat the livestock breeding wastewater, which can effectively remove the suspended particulate matter in the livestock breeding wastewater, reduce the ammonia nitrogen content, and efficiently degrade the organic matter, thereby achieving the purpose of rapid decolorization.

[0103] The test results of Examples 2, 10-13 show that with the increase of the amount of anaerobic bacteria agent, the organic matter concentration and the suspended solids content of the obtained effluent water show a trend of first decreasing and then increasing, the ammonia nitrogen content shows a trend of gradually decreasing, and the color shows a trend of first decreasing and then basically remaining unchanged. Therefore, considering the economic cost, the application further controls the amount of anaerobic bacteria agent to be 50-150 g / m 3 , so as to obtain effluent water with low organic matter, ammonia nitrogen, suspended solids content and color.

[0104] Although the present application has been described in detail with general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application claimed.

Claims

1. A method for decolorizing livestock and poultry breeding wastewater, characterized in that, The method comprises the following steps: pretreatment, anaerobic treatment, flocculation, and sedimentation; The anaerobic treatment is performed by adding an anaerobic bacteria agent to the pretreated livestock and poultry breeding wastewater and treating for 24-48 hours. The anaerobic bacteria agent comprises the following components in parts by weight: 1-2 parts of Pseudomonas fluorescens, 0.3-0.8 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium longum, and 2-5 parts of lactic acid bacteria; and the viable bacterial count of the anaerobic bacteria agent is ≥ 500 billion CFU / g. The amount of anaerobic bacteria agent added is 50-150 g / m 3 .

2. The method of decolorizing livestock and poultry breeding wastewater according to claim 1, characterized in that, The anaerobic bacteria agent further comprises 5-10 parts of an activator.

3. The method of decolorizing livestock and poultry breeding wastewater according to claim 2, characterized in that, The activator is selected from one or more of ethylenediamine phosphate, propylenediamine phosphate, dimethylbenzene, sodium fluorosilicate, ammonium sulfate, ammonium chloride, ferrous sulfate, and ammonium hydroxide.

4. The method of claim 1, wherein the wastewater is livestock wastewater. The pretreatment is performed by three-stage filtration, and the filter pore sizes of the three-stage filtration are 3-4 mm, 2-3 mm, and 1-2 mm, respectively.

5. The method of claim 1, wherein the wastewater is livestock wastewater. The flocculation step uses a flocculant selected from one or more of polyaluminum chloride, polyferric sulfate, aluminum sulfate, and anionic polyacrylamide.

6. The method of decolorizing livestock and poultry breeding wastewater according to claim 1, characterized in that, The sedimentation treatment is performed by using a sloping plate for 1-3 hours.

7. The method of decolorizing livestock and poultry breeding wastewater according to any one of claims 1 to 6, characterized in that, The livestock and poultry breeding wastewater after the sedimentation treatment is filtered by using a 0.45-μm microporous filter membrane.

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