Method for binding waste rubber to slope protection with amylase and lignin

Abstract

The application discloses a slope protection method combining waste and old rubber with lignin and amylase, which comprises the following steps: step 1), removing the gravel and household garbage on the surface of the accumulated slope, digging water guide ditches at equal intervals for filling, sowing seeds into planting holes, laying rubber particles and biomass charcoal, and leveling the soil body; step 2), spraying the amylase mixed solution on the slope surface by using a spraying machine at the planting hole of the plant, then spraying the calcium source mixed solution and the lignin on the slope surface in proportion, repeatedly spraying every interval of time, and finally laying a two-dimensional protection net; step 3), after the seeds germinate, the solution in step 2) is sprayed again in sequence, and finally non-woven fabric is covered; the application combines the waste and old rubber tire with the biomineralization technology to achieve the purpose of repairing the accumulated slope, and solves the problems that the existing slope protection technology cannot be properly disposed, the greening effect is poor, and the technology is not environmentally friendly.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of slope ecological protection engineering, in particular to a slope protection method combining waste and old rubber with amylase and lignin. BACKGROUND

[0002] With the rapid advancement of modernization, the continuous improvement of people's living standards has brought about the vigorous development of the logistics industry. Almost every family in the city has a car, which has caused insufficient road capacity and the continuous production of automobile garbage, especially the consumption of automobile tires. In order to improve energy utilization efficiency and strengthen the carbon sink capacity of the ecological system, how to properly handle waste and old rubber tires has become a hot issue of concern from all walks of life.

[0003] However, the existing waste and old rubber tire recycling methods include physical methods and chemical methods. The physical method includes tire retreading and crushing recycling. The technical level of the recycled retreaded tire is low, but the tire elasticity is poor, which accelerates the aging of the rubber, the labor intensity is large, and the secondary pollution problem is prominent. The chemical method includes devulcanization recycling. The recycled resource value is high, but traditional devulcanization recycling will lead to rubber crosslinking network, high technical level and high energy consumption. The product result is greatly affected by the process. The conventional vegetation slope protection has the disadvantage of weak anti-scouring ability. Therefore, it is necessary to find an environmentally friendly, friendly and convenient method to treat waste and old rubber tires and effectively reinforce the slope to improve the anti-scouring property, which will bring great economic and social benefits to the ecological construction and environmental improvement of our country. SUMMARY

[0004] The purpose of the present application is to overcome the above-mentioned shortcomings and provide a slope protection method combining waste and old rubber with amylase and lignin. The purpose of the present application is to achieve the purpose of repairing the accumulated slope by combining waste and old rubber tires with biomineralization technology. The present application solves the problems of poor greening effect and environmental protection of the existing slope protection technology.

[0005] To solve the above technical problems, the technical solution adopted by the present application is as follows: a slope protection method combining waste and old rubber with amylase and lignin, which comprises the following steps:

[0006] Step 1), removing the rubble and household garbage on the surface of the accumulated slope, digging water guide ditches at equal intervals for filling, sowing seeds into planting holes, laying rubber particles and biomass charcoal, and leveling the soil body;

[0007] Step 2), using a spraying machine to spray amylase mixed solution on the slope surface at the plant planting hole, then mixing and spraying calcium source mixed solution and lignin on the slope surface in proportion, and repeating the spraying every certain period of time, and finally laying two-dimensional protection net;

[0008] Step 3), after the seeds germinate, the solution in step 2) is sprayed again in sequence, and finally non-woven fabric is covered.

[0009] Preferably, in the step 1), the seed is tall fescue seed, which is washed with pure water to remove impurities; the gibberellin is dissolved in ethanol first, then diluted with pure water to 200-220 mg / L, and the seed is soaked in the treated gibberellin solution for 10-12 hours; the seed is reserved for use after soaking; the seed is sown at a density of 6-10 g / m 2 , and the distance between adjacent seeds is 13-17 cm, and the distance between adjacent planting holes is 13-17 cm.

[0010] Preferably, in the step 1), the water guide ditch is arranged obliquely, the top water guide ditch is spaced apart from the ridge by 80-120 cm, has a width of 10-15 cm, and has a depth of 8-12 cm, adjacent water guide ditches are spaced apart by 80-120 cm, and the water guide ditch is filled with palm net, and adjacent palm nets are fixed by U-shaped nails.

[0011] Preferably, in the step 1), the biomass charcoal is corn straw biomass charcoal purchased from the market; the rubber particles are obtained by processing waste tires, and the processing method is as follows: the steel wire and tire in the rubber tire are separated, a crusher is used for first crushing, the crushing process is maintained at a temperature of 25-30℃, then the rubber coarse particles of centimeter level are obtained through multiple crushing, and then the rubber fine particles of 1-2 mm are obtained through grinding and sieving by a rubber powder mill, and the rubber particles are obtained after soaking in 40℃ water to remove metal impurities; finally, the rubber particles are mixed with the corn straw biomass charcoal, and a spraying machine is used for laying, the mixing mass ratio is 1.5:1, and the laying density is 10-15 g / m 2 .

[0012] Preferably, in the step 2), the amylase mixed solution is a mixture of urease crude solution and alpha amylase, and the extraction method of the urease crude solution is as follows: soybeans are dried in an oven at 30℃ for 8 hours, the soybeans are cooled to room temperature, the soybeans are crushed by a cell wall crusher and passed through a 100 mesh sieve, the soybean powder is added to a 30% ethanol solution at a concentration of 60 g / L, then the suspension is stirred for 30 min to obtain a uniform suspension, the suspension is filtered through cheese cloth and stored in a 4℃ refrigerator for 12 h to obtain a supernatant layered solution, and the supernatant layered solution is centrifuged at 10000 rpm / min for 10 min at 4℃, and finally the supernatant containing urease is collected as the urease crude solution; the urease crude solution and alpha amylase are mixed at a mass ratio of 2:1 to obtain the amylase mixed solution.

[0013] Preferably, in the step 2), the extraction method of the calcium source mixed solution is: roasting the sesame at 30-40 DEG C, washing the surface shell and impurities with pure water, grinding into powder with a grinder, screening to obtain sesame powder through 80-120 meshes, mixing the carbamide and the sesame powder with pure water in a mass ratio of 1:1, and stirring to obtain the calcium source mixed solution.

[0014] Preferably, in the step 2), the volume ratio of the amylase mixed solution and the calcium source mixed solution is 1:1, and the mass of the lignin is 3%-4% of the total mass of the carbamide and the sesame powder in the calcium source.

[0015] Preferably, in the step 2), the amount of the amylase mixed solution and the calcium source mixed solution sprayed on the slope surface each time is 2.0-2.4 L / m 2 , 1.0-1.2 L / m 2 , and the interval time of spraying is 4-5 days, and the spraying is continued for 12-15 days.

[0016] Preferably, in the step 2), the two-dimensional protection net adopts a starch-based fiber net as a raw material, is laid from top to bottom according to the stress direction of the fiber net on the slope surface, is reserved 30-40 cm from the top of the slope, and is laid to the bottom of the slope, each region is fixed by using a fiber turf nail, the distance between the net line and the slope surface is 5-10 cm, and the adjacent nets are treated by using a lap joint form, and the lap joint is inserted by using a straw strip.

[0017] Preferably, in the step 3), the amount of the amylase mixed solution and the calcium source mixed solution sprayed in sequence is changed to 1.0-1.2 L / m 2 , 0.5-0.6 L / m 2 , and the non-woven fabric is removed when the plant grows to 4 cm.

[0018] The present application has the following beneficial effects:

[0019] (1) The alpha amylase and urease are mixed in the present application, firstly, the addition of the amylase participates in the decomposition process of the soil organic matter and humus, the macromolecular organic matter is decomposed into sugar substances which are more easily absorbed and utilized by plants, the penetration depth can reach more than 2 cm above the ground, and the nutrient circulation in the soil is promoted, secondly, the addition of the amylase can promote the decomposition of the carbamide by the urease to provide cell energy and carbon source, promote the normal catalytic function of the urease, and achieve the effect of accelerating the plant growth.

[0020] (2) In this invention, the addition of lignin ensures that only one type of homogeneous polymorphism is generated by enzyme-induced calcium carbonate mineralization, namely the most stable calcite thermodynamic phase, and provides nucleation sites for enzyme-induced calcium carbonate precipitation technology; the furfural ring composed of benzene ring and furfural group contained in lignin combines with calcite to form a chain-like cement. This structure has good stability and can improve the resistance of loose soil particles to liquefaction and deformation, eliminating the hidden danger of structural damage caused by high temperature in engineering measures from the inside; in addition, the addition of lignin will promote the accumulation of organic matter in the soil and improve soil fertility; on the other hand, the flavonoids in lignin will combine with soil particles to enhance the structural stability, wind erosion resistance and water retention capacity of soil aggregates.

[0021] (3) The addition of corn straw biochar in this invention enables the small soil particles to connect with each other, and the organic matter remaining in the corn straw biochar also provides nutrients for plant growth. When calcium carbonate is generated in the soil pore space, the high specific surface area of ​​corn straw biochar can make the unreacted calcium source solution react completely, and the porous structure can also serve as a nucleation site for calcium carbonate, thereby improving the generation efficiency of calcium carbonate. In addition, the attraction generated by the CO bonds in the biochar will cause the enzyme-induced calcium carbonate to grow in parallel, thereby forming more dense hemispherical crystals embedded in the pores of soil particles.

[0022] (4) This invention reuses waste tires as a pollution-free raw material to improve the strength of embankment slopes, which to some extent solves the risks of high technical difficulty and large environmental pollution problems in waste tire recycling. The aggregation of rubber particles actually fills the pores inside the soil, forming a weak interface. Some rubber particles will replace soil particles to bear the load of the skeleton, reducing the compressive strength of the soil. The introduction of enzyme-induced calcium carbonate mineralization technology can make up for this shortcoming. The addition of rubber particles can improve the pore structure of the soil. Biochar, rubber particles and soil particles are firmly attached together, improving the aeration and permeability of the soil, and better releasing or adsorbing nutrients in the soil through exchange, improving the cohesion and internal friction angle of the soil, and maintaining the integrity of the soil and the water and soil conservation performance.

[0023] (5) This invention uses sesame as a calcium source, which can effectively increase the rate of enzyme-induced calcium carbonate reaction, and the calcium carbonate production increases by about 2-3 times; it solves the problem that when anhydrous calcium chloride is used as a calcium source, the pH rises to a weakly alkaline state, that is, the reaction between urea and soybean urease causes the soil pH to be weakly alkaline, and the activation of urease at room temperature causes the pH to drop slightly, and then rises to a weakly alkaline state after a few days, which is not suitable for the soil conditions for the growth of most plants; the organic acids and other substances in sesame powder will neutralize the alkalinity produced, so that the soil pH can be adapted to the survival conditions of most herbaceous plants; in addition, the addition of sesame also helps to reduce the problem of the temperature rising too quickly during the reaction process.

[0024] (6) In the enzyme-induced calcium carbonate mineralization technology used in this invention, the interaction between the hydroxyl polar groups contained in the compound and lignin will damage the dense spherical supramolecular microregions in lignin, giving it a honeycomb-like porous structure. These pores can carry calcite particles and form a double helix structure with lipid molecules in rubber particles, generating strong intermolecular forces within the biomacromolecules.

[0025] (7) The palm mesh used in this invention is used as a filling material in the inclined drainage ditch. Palm mesh has many advantages such as low price, easy availability, high water resistance, light resistance, water permeability, and air permeability. Moreover, the interlaced spacing of the palm mesh layers can effectively prevent soil erosion and provide a certain degree of support. The two-dimensional protective net material is a starch-based fiber net, which is a biodegradable material that can be biodegraded in the natural environment and is environmentally friendly. The spraying of amylase can decompose part of the net structure into sugar molecules, which can serve as nutrients for plant growth. In terms of slope protection, the starch-based fiber net firstly... It is highly adaptable to slope terrain, has a short deployment cycle, high construction efficiency, small workload, fast construction progress, and minimal damage to the original topography; secondly, it requires less labor and has a lower project cost, making it more economical and economical, and eliminating the need for large-scale cleanup later; finally, the mesh structure can resist rainwater erosion, and during droughts, it can collect dew at night to provide short-term water for plants, serving as an attachment material for tall fescue growth and improving survival rate; the interspersed straw strips can act as plant fibers, improving the adhesion between soil particles (serving as a medium for spraying substrate), and after natural degradation and shedding, they can serve as organic matter in the soil to ensure plant growth.

[0026] (8) This invention combines enzyme-induced calcium carbonate mineralization technology with waste tires and lignin for effective waste utilization. The treatment of slopes is based on not destroying the original broken structure. The materials are widely available and can be fully utilized for industrial mass production and promotion, increasing the survival rate of vegetation on slopes and reducing the soil erosion characteristics such as wind erosion and runoff scouring that are susceptible to accumulation slopes.

[0027] (9) This invention combines waste rubber tires with biomineralization technology to achieve the purpose of repairing the accumulated slope, which solves the shortcomings of existing slope protection technology that cannot be properly disposed of, has poor greening effect and is not environmentally friendly. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the slope protection structure involved in the present invention;

[0029] Figure 2 for Figure 1 Enlarged structural diagram of the area where the medium-fiber turf nails are located;

[0030] Figure 3 for Figure 1 A schematic diagram of the right-side view structure;

[0031] In the figure, 1 represents a planting hole; 2 represents a water guide ditch; 3 represents a slope surface; 4 represents a U-shaped nail; 5 represents a palm net; 6 represents a fiber turf nail; 7 represents a two-dimensional protective net; and 8 represents a straw strip. DETAILED DESCRIPTION

[0032] The application will be further described in detail below in combination with the drawings and specific examples.

[0033] As shown in the figure, a slope protection method for combining waste and old rubber with amylase and lignin comprises the following steps: Figures 1 to 3 Step 1), removing the rubble and household garbage on the surface of the accumulated slope, digging water guide ditches at equal intervals for filling, planting holes for sowing seeds, laying rubber particles and biomass charcoal, and leveling the soil body;

[0034] Step 2), using a spraying machine to spray the amylase mixed solution on the slope surface at the plant planting hole, then mixing and spraying the calcium source mixed solution and lignin on the slope surface in proportion, and repeating the spraying at intervals, and finally laying the two-dimensional protective net;

[0035] Step 3), after the seeds germinate, the solutions in step 2) are sprayed again in sequence, and finally the non-woven fabric is covered.

[0036] In the step 1), the plant seeds are tall fescue seeds, which are purchased from the market and washed with pure water to remove impurities. First, gibberellin is dissolved with ethanol, and then pure water is added to dilute it to 200-220 mg / L. The seeds are soaked in the treated gibberellin solution for 10-12 hours, and the soaked seeds are reserved for use. The sowing density of the seeds is 6-10 g / m 2 , the distance between adjacent seeds is 13-17 cm, and the distance between adjacent planting holes is 13-17 cm.

[0037] In the step 1), the biomass charcoal is derived from corn straw biomass charcoal purchased from the market, and the rubber particles can be processed by purchasing waste tires from the market. The processing method is as follows: first, separate the steel wire and tire from the rubber tire, use a crusher for the first crushing, and keep the temperature between 25-30°C during the crushing process. Then, after multiple crushing, cm-level rubber coarse particles are obtained, which are ground and sieved by a rubber powder mill to obtain 1-2 mm rubber fine particles. After soaking in 40°C water to remove metal impurities, rubber particles are obtained. Finally, the rubber particles are mixed with corn straw biomass charcoal, and then laid using a spraying machine, with a mixing mass ratio of 1.5:1 and a laying density of 10-15 g / m 2 .

[0038]

[0039] ​In the step 2), the extraction method of the amylase mixture is as follows: soybean and alpha amylase product are purchased in the market. The soybean is dried in an oven at 30°C for 8 hours, and then cooled to room temperature. The soybean is crushed by a cell breaker and sieved through a 100-mesh sieve. The soybean powder is added into a 30% ethanol solution at a concentration of 60 g / L with a mass ratio of 1:10. Then, the suspension is stirred for 30 min to obtain a uniform suspension, which is filtered through a cheese cloth and stored in a refrigerator at 4°C for 12 h to obtain a supernatant layered solution. The supernatant layered solution is centrifuged at 10,000 rpm / min at 4°C for 10 min. Finally, the supernatant containing urease is collected as a crude urease solution. The crude urease solution and alpha amylase are mixed at a mass ratio of 2:1 before mixing to obtain the amylase mixture.

[0040] In the step 2), the extraction method of the calcium source mixture is as follows: the sesame purchased in the market is baked at 30-40°C, and then washed with pure water to remove the surface shell and impurities. The sesame is ground into powder using a grinder and sieved through a 80-120-mesh sieve to obtain sesame powder. Carbamide and the sesame powder are mixed with pure water at a mass ratio of 1:1 to obtain the calcium source mixture.

[0041] In the step 2), the volume ratio of the amylase mixture to the calcium source mixture is 1:1, and the mass of the lignin is 3%-4% of the total mass of the carbamide and the sesame powder in the calcium source.

[0042] In the step 2), the amount of the amylase mixture and the calcium source mixture sprayed onto the slope surface each time is 2.0-2.4 L / m 2 , 1.0-1.2 L / m 2 , respectively, and the interval time of spraying is 4-5 days, and the spraying is continued for 12-15 days.

[0043] In the step 3), the amount of the amylase mixture and the calcium source mixture sprayed in turn is changed to 1.0-1.2 L / m 2 , 0.5-0.6 L / m 2 , respectively, and the ratio is unchanged. When the plant grows to 4 cm, the non-woven fabric can be removed.

[0044] The following specific examples are used to illustrate the application.

[0045] Example 1 (the application)

[0046] Taking a slope land near a pumped storage power station as an example, a 5m x 5m sample land is taken from a 1# slope with a height of 18m and a slope of 35°. In step one, the debris and household garbage on the surface of the accumulated slope are removed. In step two, a seed planting hole is dug every 16 cm, and high fescue seeds soaked in pure water and gibberellin are sown at a density of 8 g / m 2, step three, spray rubber particles and biomass charcoal mixture on the sample plot, the ratio is 1.5:1, the laying density is 15g / m 2 , every 5 days, repeat spraying amylase mixture and lignin mixed solution, calcium source mixture, the concentration is 2.4L / m 2 and 1.2L / m 2 , after the seed germination, spray the above-mentioned substances again, the concentration is changed to 1.2L / m 2 and 0.6L / m 2 , before each spraying, detect the organic matter content, unconfined compressive strength, shear strength under low vertical pressure, soil separation capacity, vegetation coverage, porosity and tall fescue plant number per square meter of the soil.

[0047] Comparative example 1 (without amylase)

[0048] Taking a 5m*5m sample plot on a 18m high and 35° slope of a 2# slope of a slope greening construction site near a certain pumped storage power station as an example, step one, remove the rubble and household garbage on the surface of the accumulated slope, step two, dig out seed planting holes every 16cm, sow tall fescue seeds washed with pure water and soaked with gibberellin, the sowing density is 8g / m 2 , step three, spray rubber particles and biomass charcoal mixture on the sample plot, the ratio is 1.5:1, the laying density is 15g / m 2 , every 5 days, repeat spraying equal amount of water and lignin mixed solution, calcium source mixture, the concentration is 1.2L / m 2 and 1.2L / m 2 , after the seed germination, spray the above-mentioned substances again, the concentration is changed to 0.6L / m 2 and 0.6L / m 2 , before each spraying, detect the organic matter content, unconfined compressive strength, shear strength under low vertical pressure, soil separation capacity, vegetation coverage, porosity and tall fescue plant number per square meter of the soil.

[0049] Comparative example 2 (without rubber particles and biomass charcoal)

[0050] Taking a 5m*5m sample plot on a 18m high and 35° slope of a 3# slope of a slope greening construction site near a certain pumped storage power station as an example, step one, remove the rubble and household garbage on the surface of the accumulated slope, step two, dig out seed planting holes every 16cm, sow tall fescue seeds washed with pure water and soaked with gibberellin, the sowing density is 8g / m 2 , step three, every 5 days, repeat spraying amylase mixture and lignin mixed solution, calcium source mixture, the concentration is 2.4L / m 2 and 1.2L / m 2, and after the seeds germinate, the above-mentioned substances are sprayed again, and the concentration is changed to 1.2 L / m 2 and 0.6 L / m 2 Before each spraying, the organic matter content, unconfined compressive strength, shear strength under low vertical pressure, soil separation capacity, vegetation coverage, porosity, and tall fescue plant number per square meter of the soil are detected.

[0051] Comparative Example 3 (no calcium source solution and lignin)

[0052] Taking a 5m x 5m sample plot on a 4# slope with a height of 18m and a slope of 35° of a slope greening construction site near a certain pumped storage power station as an example, step one, remove the rubble and household garbage on the surface of the accumulated slope, step two, dig out seed planting holes every 16cm, and sow tall fescue seeds washed with pure water and soaked with gibberellin, with a sowing density of 8g / m 2 , step three, spray a mixture of rubber particles and biomass charcoal on the sample plot, with a ratio of 1.5:1, and a laying density of 15g / m 2 Repeat the spraying of amylase mixed solution every 5 days, with a concentration of 1.2 L / m 2 and an equal amount of water, and after the seeds germinate, the above-mentioned substances are sprayed again, and the concentration is changed to 0.6 L / m 2 Before each spraying, the organic matter content, unconfined compressive strength, shear strength under low vertical pressure, soil separation capacity, vegetation coverage, porosity, and tall fescue plant number per square meter of the soil are detected.

[0053] Comparative Example 4 (blank)

[0054] Taking a 5m x 5m sample plot on a 5# slope with a height of 18m and a slope of 35° of a slope greening construction site near a certain pumped storage power station as an example, step one, remove the rubble and household garbage on the surface of the accumulated slope, step two, dig out seed planting holes every 16cm, and sow tall fescue seeds washed with pure water and soaked with gibberellin, with a sowing density of 8g / m 2 , step three, repeat the spraying of water every 5 days, and after the seeds germinate, spray water again, and before each spraying, the organic matter content, unconfined compressive strength, shear strength under low vertical pressure, soil separation capacity, vegetation coverage, porosity, and tall fescue plant number per square meter of the soil are detected.

[0055] Comparing the above examples and comparative examples, the data are as follows:

[0056]

[0057] Test method and reference standard: organic matter is determined by using a flow analyzer; unconfined compressive strength is measured by using a universal testing machine according to the standard for soil test methods (GB / T 50123-2019); shear strength under low vertical pressure is measured by using a direct shear apparatus under low vertical pressure (25 kPa) according to the standard for soil test methods (GB / T 50123-2019); soil separation capacity is measured by using a simulated scouring test; vegetation coverage is measured by using a sampling needle puncture method to calculate the percentage of the vertical projection area of the above-ground part of the total plant community or each individual to the sample area; porosity is determined by using a density test method according to the standard for soil test methods (GB / T 50123-2019); and the number of tall fescue plants per square meter is measured by using a visual counting method.

[0058] As can be seen from the above comparative data, the vegetation coverage of the example 30 days after the treatment is 1.73-2.69 times higher than that of the control example, the unconfined compressive strength and shear strength under 25 kPa of the soil body are increased by 105%-413% and 51%-184%, respectively, the porosity is reduced by 13.8% before 30 days, the content of organic matter in the soil body is increased by about 2.9 times at most, the soil separation capacity, which represents the ability of the soil to resist rainwater runoff and scouring, is reduced by 43% compared with the blank group after 30 days, and the number of dogtooth grass plants per square meter is also greatly increased compared with the control group. It is shown that the slope protection method of combining the starch amylase with the waste and old rubber and the lignin can significantly improve the soil erosion resistance and mechanical strength and other soil and water conservation capacity indicators of the soil body, the increase of the organic matter content provides a relatively mild environment for the subsequent growth of the vegetation on the slope, the large-area coverage of the vegetation can effectively improve the effect of the slope greening recovery and management, and the reuse of the waste materials also plays a due role in reducing the greenhouse effect.

[0059] The above examples are only preferred technical solutions of the present application, and should not be regarded as a limitation of the present application. The protection scope of the present application should be based on the technical solutions recited in the claims, including equivalent replacement solutions of the technical features recited in the claims. That is, equivalent replacement improvements within this range are also within the protection scope of the present application.

Claims

1. A method for slope protection using amylase in synergistic lignin binding with waste rubber, characterized in that: It includes the following steps: Step 1) Remove the gravel and domestic waste from the surface of the slope, dig drainage ditches at equal intervals and fill them, sow seeds in the planting holes, lay rubber granules and biochar, and level the soil. Step 2) Use a sprayer to spray the amylase mixture onto the slope at the planting holes, then mix the calcium source mixture and lignin in proportion and spray it onto the slope. Repeat the spraying at intervals, and finally set up a two-dimensional protective net. Step 3) After the seeds sprout, spray the solution from Step 2) again in sequence, and finally cover with non-woven fabric; In step 1), the seeds are tall fescue seeds, which are washed with pure water to remove impurities; gibberellin is first dissolved in ethanol, then diluted with pure water to 200-220 mg / L, and the seeds are soaked in the treated gibberellin solution for 10-12 hours. The soaked seeds are then stored for later use; the sowing density is 6-10 g / m². 2 The spacing between adjacent seeds is 13-17 cm, and the spacing between adjacent planting holes is 13-17 cm. In step 1), the water diversion ditch is arranged at an angle, with the top water diversion ditch spaced 80-120 cm from the top of the slope, 10-15 cm wide, and 8-12 cm deep. Adjacent water diversion ditches are 80-120 cm apart. The water diversion ditch is filled with palm mesh, and adjacent palm meshes are fixed with U-shaped nails. The biochar in step 1) is sourced from corn stalk biochar purchased from the market; the rubber granules are a product obtained from the processing of waste tires. The processing method is as follows: the steel wires in the rubber tires are separated from the tires, and the tires are crushed for the first time using a crusher. The crushing process is carried out at a temperature between 25-30℃. Then, after multiple rounds of crushing, coarse rubber particles at the centimeter level are obtained. These particles are then ground in a rubber pulverizer and sieved to obtain fine rubber particles of 1-2 mm. After soaking in water at 40℃ to remove metal impurities, the rubber granules are obtained. Finally, the rubber granules are mixed with corn stalk biochar and then laid using a spraying machine. The mixing mass ratio is 1.5:1, and the laying density is 10-15 g / m³. 2 ; In step 2), the amylase mixture is a mixture of crude urease and alpha amylase. The crude urease is extracted as follows: soybeans are dried in an oven at 30°C for 8 hours, cooled to room temperature, crushed using a high-speed blender and passed through a 100-mesh sieve, and the soybean powder is added to a 30% ethanol solution with a mass ratio of 1:10 at a concentration of 60 g / L. The suspension is then stirred for 30 min to obtain a uniform suspension. After filtration through cheesecloth, the suspension is stored in a refrigerator at 4°C for 12 h to obtain a supernatant layer. The supernatant layer is then centrifuged at 10,000 rpm / min for 10 min at 4°C. Finally, the supernatant containing urease is collected as the crude urease. The crude urease and alpha amylase are mixed at a mass ratio of 2:1 to obtain the amylase mixture.

2. The method for slope protection using amylase-assisted lignin-based binding of waste rubber according to claim 1, characterized in that: In step 2), the extraction method of the calcium source mixture is as follows: sesame seeds are roasted at 30-40℃, the surface shell and impurities are washed with pure water, and the seeds are ground into powder using a grinder. The powder is then sieved through an 80-120 mesh sieve to obtain sesame powder. Carbonamide and sesame powder are mixed with pure water in a mass ratio of 1:1 and stirred to obtain the calcium source mixture.

3. The method for slope protection using amylase-assisted lignin-based binding of waste rubber according to claim 2, characterized in that: In step 2), the volume ratio of the sprayed amylase mixture and the calcium source mixture is 1:1, and the mass of lignin is 3%-4% of the total mass of carbamide and sesame powder in the calcium source.

4. The method for slope protection using amylase-assisted lignin-based binding of waste rubber according to claim 1, characterized in that: In step 2), the amount of amylase mixture and calcium source mixture sprayed onto the slope each time is 2.0-2.4 L / m². 2 1.0-1.2 L / m 2 The spraying interval is 4-5 days, and the process lasts for 12-15 days.

5. The method for slope protection using amylase-assisted lignin-based binding of waste rubber according to claim 1, characterized in that: The two-dimensional protective netting in step 2) uses starch-based fiber netting as raw material. According to the stress direction of the fiber netting on the slope, it is laid from top to bottom, leaving a 30-40 cm gap from the top of the slope, and is laid to the bottom of the slope. Each area is fixed with fiber turf nails. The distance between the netting and the slope is 5-10 cm. Adjacent netting is treated by overlapping, and straw strips are used to interweave at the overlap.

6. The method for slope protection using amylase-assisted lignin-based binding of waste rubber according to claim 1, characterized in that: In step 3), the amount of amylase mixture and calcium source mixture sprayed sequentially becomes 1.0-1.2 L / m³. 2 0.5-0.6L / m 2 Once the plant has grown to 4 cm, remove the non-woven fabric.

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