Plant growth regulator for improving yield, quality and stress resistance of rice and its use method and application

Abstract

This invention discloses a plant growth regulator that can improve the yield, quality, and stress resistance of rice, as well as its application method and application. The active ingredient of the plant growth regulator is a ternary compound composition of salicylic acid, potassium humate (mineral-derived), and pyraclostrobin. The concentration of salicylic acid is 50-200 μM, the concentration of potassium humate is 100-400 mg / L, and the concentration of pyraclostrobin is 7.5-30 mM. Preferably, the concentration of salicylic acid is 100 μM, the concentration of potassium humate is 200 mg / L, and the concentration of pyraclostrobin is 15 mM. This invention utilizes a ternary compound of salicylic acid, mineral-derived potassium humate, and pyraclostrobin as active ingredients, which can significantly improve rice yield and quality, and enhance rice's resistance to heat, low temperatures, low light, or cold. It can be used alone as a plant growth regulator to improve rice yield, rice quality, or stress resistance, or as a plant growth regulator to synergistically improve rice yield, rice quality, and stress resistance.

Description

Technical Field

[0001] This invention relates to the field of plant growth regulators, and in particular to a plant growth regulator that can improve the yield, quality and stress resistance of rice, as well as its application and usage. Background Technology

[0002] With the improvement of people's living standards in my country, the demand for high-quality rice is increasing, gradually becoming one of the main obstacles restricting grain production. Although significant progress has been made in the breeding of high-quality rice varieties in recent years, with the Nanjing series of rice reaching or approaching the level of Koshihikari rice from Japan in terms of eating quality, high-quality rice varieties often suffer from low yields and poor adaptability. The contradiction of "high yield but not high quality, and high quality but not high yield" still exists widely. In the summer of 2022, the middle and lower reaches of the Yangtze River in my country experienced the most severe high-temperature drought since 1961, resulting in severe rice yield losses and serious deterioration of rice quality. In 2023, no extreme high-temperature weather occurred, but temperatures were low during the grain-filling stage of mid- and late-season rice, with minimum temperatures even dropping below 20°C, and sunlight was significantly insufficient, leading to a significant decrease in the yield of some varieties. In addition, the head rice rate of most rice varieties decreased significantly.

[0003] In light of this, research on cultivation techniques for synergistically improving rice yield and quality is urgently needed. Numerous studies have shown that optimizing water and fertilizer management is an important way to improve rice yield and quality, and there are many related reports. However, in practice, its feasibility is not high because different varieties have different water and fertilizer utilization, and there are significant regional and climatic differences. Only after fully understanding the local soil quality and the growth habits of rice varieties can water and fertilizer regulation be effectively carried out. There are too many uncontrollable factors, the workload is too large, and it is difficult to establish a unified standard.

[0004] Exogenous plant growth regulators have shown good effects on rice yield, quality, and stress resistance, with relatively low dependence on varieties, simple application methods, and ease of promotion. Using plant growth regulators and optimizing their application methods is an important strategy. Previous studies have shown that chemical regulation can significantly improve rice yield and stress resistance. However, currently used chemical regulation products are expensive, and there are few plant growth regulators that can synergistically improve rice yield, quality, and stress resistance.

[0005] Therefore, there is an urgent need for a plant growth regulator that can synergistically improve rice yield, quality, and stress resistance. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to provide a plant growth regulator that can synergistically improve the yield, quality and stress resistance of rice, as well as its application method and application.

[0007] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0008] On the one hand, the present invention provides a plant growth regulator that can improve the yield, quality and stress resistance of rice. The active ingredients are a ternary compound composition of salicylic acid (SA), potassium humate (FA) and pyraclostrobin (PYR), wherein the concentration of salicylic acid is 50-200 μM, the concentration of potassium humate is 100-400 mg / L, and the concentration of pyraclostrobin is 7.5-30 mM.

[0009] As a further improvement of the present invention, the concentration of salicylic acid used is 100-150 μM, the concentration of potassium humate from minerals used is 200-300 mg / L, and the concentration of pyraclostrobin used is 15-22.5 mM.

[0010] Furthermore, the concentration of salicylic acid used is 100 μM, the concentration of potassium humate from minerals is 200 mg / L, and the concentration of pyraclostrobin is 15 mM.

[0011] Furthermore, the plant growth regulator also contains adjuvants to formulate a dosage form suitable for agricultural use.

[0012] Secondly, the present invention also provides a method for using a plant growth regulator that can improve rice yield, quality, and stress resistance, comprising:

[0013] Spray once during the young panicle differentiation stage and once 3-5 days before heading to improve rice yield and rice quality.

[0014] Alternatively, it can be sprayed under high temperature conditions during the meiosis stage or the heading and flowering stage to improve rice yield and rice quality while maintaining high temperature resistance.

[0015] Alternatively, it can be sprayed during the heading and flowering stage when low temperature and low light conditions are encountered, which can improve rice yield and rice quality while being resistant to low temperature and low light conditions.

[0016] Alternatively, it can be sprayed under low-temperature freezing conditions and is resistant to low-temperature freezing.

[0017] Furthermore, the high temperature is 35-40 degrees Celsius, the low temperature in the low temperature and low sunshine weather conditions is 15-25 degrees Celsius, and the low temperature in the low temperature freezing conditions is below 10 degrees Celsius.

[0018] Thirdly, the present invention provides the application of the above-mentioned plant growth regulator, wherein the application is as follows:

[0019] (1) Application in increasing rice yield;

[0020] (2) and / or, applications in improving rice quality, including reducing the chalkiness of rice and / or increasing the head rice yield of rice;

[0021] (3) and / or, applications in rice stress resistance, including heat resistance, low temperature and low light resistance, or low temperature and freezing resistance.

[0022] By adopting the above technical solution, the present invention has at least the following beneficial effects:

[0023] (1) This invention uses salicylic acid, mineral-derived potassium humate and pyraclostrobin as the three active ingredients in a ternary compound. The three ingredients work synergistically to significantly improve rice yield and rice quality, and improve the rice's resistance to heat, low temperature and low light or cold. They can be used alone as plant growth regulators to improve rice yield, rice quality or improve resistance to stress, and are preferably used as plant growth regulators to synergistically improve rice yield, rice quality and improve resistance to stress.

[0024] (2) The plant growth regulator of the present invention has a wide range of sources, low cost, low dependence on varieties, simple method of use, and is easy to promote. Attached Figure Description

[0025] The above is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] Figure 1 This is a comparison diagram of plant growth regulator treatment and control under low temperature freezing conditions in one embodiment of the present invention, wherein (a) is the result of 1.0×(SA+PYR+FA) treatment and (b) is the result of control treatment. Detailed Implementation

[0027] This invention discloses a plant growth regulator that can improve rice yield, quality, and stress resistance. The active ingredients are a ternary compound composition of salicylic acid, potassium humate (mineral-derived), and pyraclostrobin. The concentration of salicylic acid is 50-200 μM, the concentration of potassium humate is 100-400 mg / L, and the concentration of pyraclostrobin is 7.5-30 mM. More preferably, the concentration of salicylic acid is 100-150 μM, the concentration of potassium humate is 200-300 mg / L, and the concentration of pyraclostrobin is 15-22.5 mM. More preferably, the concentration of salicylic acid is 100 μM, the concentration of potassium humate is 200 mg / L, and the concentration of pyraclostrobin is 15 mM.

[0028] In addition to the active ingredient, the aforementioned plant growth regulators may also contain adjuvants to formulate formulations suitable for agricultural use. Examples include commonly used wettable powders, water-dispersible granules, microemulsions, emulsions, emulsifiable concentrates, suspensions, or granules. This embodiment preferably uses water-dispersible granules.

[0029] When using it, either drone spraying or manual spraying can be used depending on the different situations and at different times.

[0030] The following detailed description is provided through examples:

[0031] Example 1

[0032] Using Huang Huazhan as the material, different concentrations of salicylic acid (SA), potassium humate (FA), and pyraclostrobin (PYR) were sprayed individually during the young panicle differentiation stage and 3-5 days before heading under field conditions. The application rate was 30 L per mu. The results of the effects on rice yield are shown in Table 1 below.

[0033] Table 1. Effects of different concentrations of salicylic acid, potassium humate (mineral-derived), and pyraclostrobin on rice yield.

[0034]

[0035]

[0036] As shown in Table 1, when sprayed alone, the yield of salicylic acid within the range of 100-10000 μM did not differ significantly; the 200-500 mg / L potassium humate treatment had the highest yield, but the difference between the two treatments was not significant; the 15-22.5 mM pyraclostrobin treatment had a relatively high yield, but the difference between the two treatments was not significant.

[0037] Based on the principle that for comparable effects, the lower the concentration, the better, the optimal concentrations are: salicylic acid 100 μM, potassium humate 200 mg / L, and pyraclostrobin 15 mM.

[0038] Example 2

[0039] A binary or ternary compound was prepared using the optimal concentrations of salicylic acid (100 μM), mineral-derived potassium humate (200 mg / L), and pyraclostrobin (15 mM). The compound was sprayed once during the panicle differentiation stage and again 3-5 days before heading under field conditions. The effects on rice yield are shown in Table 2 below.

[0040] Table 2. Effects of different compound concentrations on rice yield.

[0041]

[0042] Table 2 shows that, based on the principle that lower concentrations are better for comparable effects, the 1.0×(SA+FA) and 1.0×(PYR+FA) treatments showed better results, with comparable yield increases in rice. Therefore, a ternary compound of salicylic acid (100 μM), potassium humate (200 mg / L), and pyraclostrobin (15 mM) was adopted. The compound of salicylic acid, potassium humate, and pyraclostrobin significantly increased rice yield, with the 1.0×(SA+PYR+FA) treatment showing the highest yield increase at 14.9%, followed by 1.5×(SA+PYR+FA) at 13.1%. The 0.5×(SA+PYR+FA) and 2×(SA+PYR+FA) treatments also showed yield increases of 7.7% and 6.1%, respectively. This indicates that the yield-increasing effect of this compound is relatively stable, and there are no phytotoxicity issues within this range, demonstrating high safety.

[0043] In addition, this compound combination can reduce the chalkiness of rice and increase the head rice yield, as shown in Table 3 below:

[0044] Table 3. Effects of different concentrations of salicylic acid, potassium humate, and pyraclostrobin on rice quality.

[0045]

[0046] Example 3

[0047] To further verify its effects, a large-scale experiment (5 mu) was conducted in the field. Spraying was performed once during the panicle differentiation stage and again 3-5 days before heading. The results showed that the combined treatment of salicylic acid (100 μM), potassium humate (200 mg / L), and pyraclostrobin (15 mM) on Huayou Yuhe, Zhehexiang 2, and Yongyou 7860 varieties all exhibited significant yield increases, with increases of 12.9%, 15.1%, and 14.0%, respectively. Furthermore, chalkiness decreased significantly, while the head rice rate increased significantly. The results are shown in Table 4 below. Table 4: Effects of the combination of salicylic acid, potassium humate, and pyraclostrobin on rice yield and quality under field conditions.

[0048]

[0049] Example 4

[0050] Regarding stress resistance, the compound combination of salicylic acid (100 μM), mineral-derived potassium humate (200 mg / L), and pyraclostrobin (15 mM) was sprayed during the meiosis stage and the heading and flowering stage, respectively, and subjected to high-temperature treatment (38℃, 7 days). The results are shown in Table 5 below.

[0051] Table 5 Effects of SA+FA+PYR on seed setting rate and thousand-grain weight of rice under high temperature

[0052]

[0053] Table 5 shows that the SA+FA+PYR treatment significantly improved the heat resistance of rice during both the pollen mother cell meiosis stage and the heading and flowering stage, and also significantly increased the seed setting rate and thousand-grain weight. This indicates that the combination of salicylic acid (100 μM), mineral-derived potassium humate (200 mg / L), and pyraclostrobin (15 mM) can effectively improve the heat resistance of rice.

[0054] In addition, under the high temperature conditions in the field in 2022, the SA+FA+PYR treatment during the heading and flowering stage showed good application effects in terms of both yield and rice quality, as shown in Table 6 below.

[0055] Table 6. Effects of SA+FA+PYR combination on yield and rice quality of mid-season rice Nangeng 46 under high temperature conditions in the field.

[0056]

[0057] As shown in Table 6, the combination of salicylic acid (100M), mineral-derived potassium humate (200mg / L), and pyraclostrobin (15mM) significantly increased yield, reduced chalkiness, and improved head rice rate, with a yield increase of 18.1%, effectively mitigating the damage to rice yield caused by high temperature heat.

[0058] Example 5

[0059] No extreme high-temperature events occurred in the middle and lower reaches of the Yangtze River rice-growing area in 2023. However, prolonged periods of low temperatures and insufficient sunlight occurred in the region from mid-to-late September to early October, severely impacting the yield and quality of late-season rice. In Fuyang, the yield of Yongyou 1540 rice was low, only 411.2 kg / mu, due to late sowing and the low temperatures and insufficient sunlight encountered during the heading and flowering stages. However, the SA+FA+PYR treatment (100 μM salicylic acid, 200 mg / L potassium humate, and 15 mM pyraclostrobin) achieved a yield of 478 kg / mu, an increase of 16.2%. Furthermore, the SA+FA+PYR treatment (100 μM salicylic acid, 200 mg / L potassium humate, and 15 mM pyraclostrobin) significantly reduced grain chalkiness but significantly increased head rice yield. The results are shown in Table 7 below.

[0060] Table 7. Effects of SA+FA+PYR on yield and rice quality of late-season rice Yongyou 1540 under low temperature and low light conditions during the flowering stage in the field.

[0061]

[0062] Example 6

[0063] Regarding stress resistance, this compound was treated under low-temperature freezing conditions (daytime temperature 12℃, nighttime temperature 4℃, treatment for 5 days). The results are as follows: Figure 1 As shown, by Figure 1 As shown in (a), rice treated with salicylic acid (100 μM), potassium humate (200 mg / L), and pyraclostrobin (15 mM) can withstand low-temperature freezing. Under conditions of water surface freezing, the leaves showed no obvious changes, while the rice in the control treatment showed obvious frost damage to the leaves. It can be seen that this compound combination can withstand low-temperature freezing.

[0064] In summary, this invention utilizes a ternary compound of salicylic acid, mineral-derived potassium humate, and pyraclostrobin as active ingredients. The synergistic effect of these three components significantly improves rice yield and quality, enhances rice's resistance to heat, low temperatures, and low light, or improves its stress resistance. It can be used alone as a plant growth regulator to improve rice yield, rice quality, or stress resistance, or as a plant growth regulator to synergistically improve rice yield, rice quality, and stress resistance.

[0065] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications, equivalent changes, or alterations made by those skilled in the art using the disclosed technical content shall fall within the protection scope of the present invention.

Claims

1. A plant growth regulator that can improve the yield, quality, and stress resistance of rice, characterized in that, The active ingredients are a ternary compound composition of salicylic acid, potassium humate (mineral-derived) and pyraclostrobin, wherein the concentration of salicylic acid is 50-200 μM, the concentration of potassium humate is 100-400 mg / L, and the concentration of pyraclostrobin is 7.5-30 mM.

2. The plant growth regulator according to claim 1, which can improve rice yield, quality, and stress resistance, is characterized in that, The concentration of salicylic acid used is 100-150 μM, the concentration of potassium humate from minerals is 200-300 mg / L, and the concentration of pyraclostrobin is 15-22.5 mM.

3. The plant growth regulator according to claim 1, which can improve rice yield, quality, and stress resistance, is characterized in that... The concentration of salicylic acid used is 100 μM, the concentration of mineral-derived potassium humate is 200 mg / L, and the concentration of pyraclostrobin is 15 mM.

4. The plant growth regulator according to any one of claims 1-3, which can improve rice yield, quality and stress resistance, is characterized in that, The plant growth regulator also contains adjuvants to formulate a dosage form suitable for agricultural use.

5. The method of using the plant growth regulator according to any one of claims 1-4, which can improve rice yield, quality and stress resistance, is characterized in that: Spray once during the young panicle differentiation stage and once 3-5 days before heading to improve rice yield and rice quality. Alternatively, it can be sprayed under high temperature conditions during the meiosis stage or the heading and flowering stage to improve rice yield and rice quality while maintaining high temperature resistance. Alternatively, it can be sprayed during the heading and flowering stage when low temperature and low light conditions are encountered, which can improve rice yield and rice quality while being resistant to low temperature and low light conditions. Alternatively, it can be sprayed under low-temperature freezing conditions and is resistant to low-temperature freezing.

6. The method of using the plant growth regulator that can improve rice yield, quality and stress resistance according to claim 5, characterized in that, The high temperature is 35-40 degrees Celsius, the low temperature under the low temperature and low sunshine conditions is below 20 degrees Celsius, and the low temperature under the low temperature and freezing conditions is below 10 degrees Celsius.

7. The application of a plant growth regulator according to any one of claims 1-4, characterized in that, The application is as follows: (1) Application in increasing rice yield; (2) and / or, applications in improving rice quality, including reducing the chalkiness of rice and / or increasing the head rice yield of rice; (3) and / or, applications in rice stress resistance, including heat resistance, low temperature and low light resistance, or low temperature and freezing resistance.

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