A vitamin E moisturizing skin cream added with peony fresh flower stock solution and a preparation method thereof

Abstract

The present application belongs to the technical field of daily cosmetics, and particularly relates to a vitamin E moisturizing skin cream added with peony fresh flower stock solution and a preparation method thereof. The moisturizing skin cream provided by the present application comprises, in weight parts, 1-10 parts of peony fresh flower stock solution fermentation liquor, and 0.1-2 parts of vitamin E, wherein the peony fresh flower stock solution fermentation liquor is obtained by two-step fermentation of peony fresh flower stock solution with a compound microbial agent of two-split yeast, Pseudoalteromonas and galactose yeast-like bacteria in sequence. The product obtained by compounding the vitamin E cream with the fermented peony stock solution fermentation liquor has good moisturizing property, improves the side effect of skin dryness caused by long-term use of vitamin E cream, and has good anti-allergy effect, and the prepared moisturizing cream has an inhibition rate of hyaluronidase of more than 80%.

Description

Technical Field

[0001] This invention belongs to the field of daily cosmetics technology, specifically relating to a vitamin E moisturizing lotion containing peony flower extract and its preparation method. Background Technology

[0002] Healthy skin requires maintaining the balance of the skin's micro-ecosystem. Currently, although there are precedents of successfully applying many microorganisms such as Bifidobacterium, Lactobacillus, Enterococcus, Escherichia coli, Bacillus subtilis, Bacillus cereus, Bacillus licheniformis, Clostridium butyricum, and yeast to the human body, there are also skin care products that claim to add microorganisms to maintain the skin's ecological balance.

[0003] In fact, adding beneficial microbial agents to skincare products is not so easy. For example, regulations in the cosmetics industry limit the total number of colonies in cosmetics to ≤1000 CFU / g (or CFU / mL) and the total number of molds and yeasts to ≤100 CFU / g (or CFU / mL). This determines that live bacteria cannot be applied to skincare products in the cosmetics industry.

[0004] Of course, some cosmetics, in order to achieve certain skin conditioning capabilities, typically use specific microorganisms to ferment raw materials and add the microbial metabolites to the cosmetics. However, there are potential problems with existing technologies:

[0005] Firstly, using mixed cultures to ferment raw materials in one step simplifies the process, but the mixed cultures may inhibit each other during fermentation, and the final effect is unclear.

[0006] Secondly, stepwise fermentation is based on the specific fermentation conditions of the strains used, such as fermentation temperature, pH value, and fermentation time. The product of the first fermentation step can be used as or partially used as the reactant for the second fermentation step. Compared with the blindness of mixed fermentation, stepwise fermentation process is more precise and the target product composition is clearer.

[0007] Peony, a plant of the Ranunculaceae family and Paeonia genus, has excellent ornamental and cosmetic value. Traditional ornamental peonies have multi-layered petals and produce almost no peony seeds, so their petals are widely used.

[0008] "Fengdan" peony is a classic traditional peony variety. Its petals were approved as a new resource food ingredient in 2013. Compared to other peony varieties familiar to the public, "Fengdan" peony flowers are single-petaled and almost entirely single-layered. It produces a large quantity of peony seeds, primarily cultivated as an oilseed crop. However, the development and utilization of Fengdan peony flowers are still in their early stages. Fengdan peony flowers are rich in protein, flavonoids, total ascorbic acid, anthocyanins, and minerals such as potassium, sodium, magnesium, calcium, iron, and phosphorus. The amino acid composition is excellent and well-balanced, with high levels of essential and flavor-enhancing amino acids. Furthermore, it exhibits no acute oral toxicity, making it a valuable natural health food resource.

[0009] Heze, Shandong, is known as the capital of peonies. According to official statistics, the planting area of ​​"Fengdan" peony has reached 480,000 mu. However, most peony enterprises only rely on the sale of peony seed oil to obtain economic benefits, and peony flowers can only be used as field waste, which not only wastes resources but also pollutes the environment.

[0010] Vitamin E lotion is a widely used skincare product, but it generally has certain side effects during use. That is, vitamin E acts like a hormone in the human body, and long-term external use of vitamin E may cause skin dependence. Once discontinued, it is easy to experience rebound effects such as dry skin, and it may even cause clogged pores. Currently, no commercially available vitamin E lotion has solved this problem.

[0011] If we can process peony petals at specific stages based on the accumulation patterns of small molecule active substances during different developmental stages of peony flowers, and then combine them with vitamin E lotion to improve the side effects of long-term use of vitamin E lotion, obtaining a moisturizing skin lotion would be of great significance for the high-value development of "Fengdan" peony. Summary of the Invention

[0012] To address the aforementioned technical problems, this invention provides a vitamin E moisturizing lotion containing peony flower extract, comprising, by weight, 1-10 parts of peony flower extract fermentation liquid and 0.1-2 parts of vitamin E; the peony flower extract fermentation liquid is obtained by fermenting peony flower extract twice; wherein the microbial agent used in the first fermentation is Bifida ferment lysate, and the microbial agent used in the second fermentation is a compound microbial agent, including Pseudomonas aeruginosa and Galactomyces-like bacteria.

[0013] Preferably, in the vitamin E moisturizing lotion with added peony flower extract, the weight ratio of peony flower extract to vitamin E is 6:1.

[0014] Preferably, in the first fermentation, the weight of Bifida Ferment Lysate added accounts for 0.1-0.2% of the weight of the original peony flower extract, and the enzyme activity of Bifida Ferment Lysate is ≥80,000 U / g.

[0015] Preferably, in the second fermentation, the added weight of *Pseudomonas pseudoalteromonas* accounts for 0.1-0.2% of the weight of the first fermentation broth, with an enzyme activity ≥70,000 U / g, and the added weight of *Galactomyces* accounts for 0.1-0.2% of the weight of the first fermentation broth, with an enzyme activity ≥80,000 U / g.

[0016] More preferably, the inoculation weight ratio of *Pseudomonas alterniflora* to *Galactomyces* is 2–4:3–6.

[0017] In this invention, the purpose of fermenting peony flower extract with Bifida Ferment Lysate is to obtain Bifida Ferment Lysate fermentation product lysate derived from peony, referred to as the first fermentation broth. The first fermentation broth is then subjected to ultrasonic treatment. The cavitation effect of ultrasound thins the mass transfer boundary of the first fermentation broth, accelerates the movement of active solute molecules at a lower temperature, which is beneficial for the diffusion of microbial agents in the reaction system during the second fermentation and helps to increase the rate of biological reaction.

[0018] In addition, the aforementioned vitamin E moisturizing lotion containing peony flower extract also includes the following ingredients: 1-10 parts glycerin, 0.5-2 parts hydroxyethyl urea, 1-5 parts niacinamide, 1-5 parts dimethicone, 0.5-3 parts peony (PAEONIA SUFFRUTICOSA) seed oil, 0.5-5 parts hydroxyethyl piperazine ethane sulfonic acid, 0.5-2 parts cetearyl alcohol, 0.1-0.5 parts propylene glycol, 0.01-0.1 parts Eucommia ulmoides bark extract, 0.01-0.1 parts Chrysanthemum indicum extract, 0.1-1 parts 1,2-hexanediol, 0.01-0.05 parts Lonicera japonica extract, and Panax notoginseng (Panax notoginseng) extract. Notoginseng root extract 0.01-0.05 parts, inositol 0.2-2 parts, phytosterol oleate 0.5-2 parts, glyceryl stearate 0.1-0.5 parts, sodium stearoyl lactylate 0.1-0.5 parts, 3-O-ethyl ascorbic acid 0.1-1 parts, p-hydroxyacetophenone 0.1-1 parts, polyacrylamide dimethyl taurine 0.1-1 parts, xanthan gum 0.05-0.5 parts, carbomer 0.05-0.5 parts, sodium hyaluronate 0.01-0.05 parts, water to make up to 100 parts, all of the above are parts by weight.

[0019] This invention also provides a method for preparing the above-mentioned vitamin E moisturizing lotion with added peony flower extract, comprising the following steps:

[0020] (1) Preparation of peony flower raw liquid fermentation broth;

[0021] S1. Select peony buds, wash them, and physically press them to obtain peony flower extract;

[0022] S2, sterilize the peony flower extract obtained in S1, then add bifida ferment lysate for the first fermentation, sterilize and filter after fermentation to obtain the first fermentation liquid;

[0023] S3, the first fermentation broth obtained in S2 is subjected to ultrasonic treatment, and then a compound microbial agent is added for a second fermentation. The compound microbial agent is composed of Pseudomonas alterniflora and Galactomyces-like bacteria. During the fermentation, ultrasonic treatment is performed once every 3 hours for 10 to 30 minutes each time to obtain the second fermentation broth.

[0024] S4. The second fermentation broth obtained in S3 is inactivated, filtered, and centrifuged to obtain the original fermentation broth of peony flowers.

[0025] (2) The peony flower extract fermentation liquid prepared in (1) is combined with vitamin E and other excipients to obtain a vitamin E moisturizing lotion with added peony flower extract.

[0026] Preferably, in the above preparation method, in S1, the peony is "Fengdan" peony, the peony bud is a peony bud in the round peach stage, and the diameter of the bud is 2.5 to 5.5 cm.

[0027] In fact, the inventors have found that the flavonoid content of Fengdan peony is significantly higher than that of general ornamental peony varieties. Furthermore, the flavonoid content of Fengdan peony varies significantly at different developmental stages. During the bud stage, the content of total phenols, flavonoids, and anthocyanins is the highest, especially the flavonoid content, which is as high as 528.15 U / g.

[0028] Preferably, in S2, the temperature of the first fermentation is 36-40℃, the pH is 6.0-7.0, and the fermentation time is 10-12 hours.

[0029] Preferably, in S3, the inoculation weight ratio of *Pseudomonas pseudoalteromonas* to *Galactomyces* is 2–4:3–6.

[0030] Preferably, in S3, the inoculation weight ratio of *Pseudomonas pseudoalteromonas* and *Galactomyces* is 3:5.

[0031] Preferably, in S3, the temperature of the second fermentation is 32-40℃, the pH is 4.0-6.0, and the fermentation time is 36-72h.

[0032] Preferably, the ultrasonic treatment described in S3 specifically involves ultrasonically treating the first fermentation broth obtained in S2 at a temperature of 25–40°C and a frequency of 80–120 kHz for 2–3 minutes.

[0033] More preferably, the second fermentation conditions described in S3 are as follows: at 40°C, the pH is adjusted to 5.0, and a compound microbial agent is added for the second fermentation. During the fermentation, an ultrasonic vibrator is used to sonicate the cells once every 3 hours for 25 minutes each time, and the fermentation lasts for 72 hours.

[0034] Preferably, the Bifida Ferment Lysate, Pseudomonas alterniflora, and Galactomyces-like bacteria used in this invention were all purchased from Zhejiang Aoda Pharmaceutical Technology Co., Ltd.

[0035] Furthermore, the present invention provides a vitamin E moisturizing lotion with added peony flower extract, prepared by the following method:

[0036] (1) Preparation of peony flower raw liquid fermentation broth;

[0037] S1. Select peony buds with a diameter of 2.5-5.5cm at the round peach stage, wash them, and physically press them to obtain the original juice of fresh peony flowers. No external water is added during the physical pressing process.

[0038] S2, sterilize the peony flower extract obtained in S1, adjust the pH to 6.0-7.0 at 36-40℃, add Bifida ferment lysate for the first fermentation, ferment for 10-12 hours, sterilize and filter after fermentation to obtain the first fermentation liquid;

[0039] S3, the first fermentation broth obtained in S2 is treated with ultrasound at 25-40℃ and 80-120kHz for 2-3 minutes, then the temperature is adjusted to 32-40℃ and the pH is 4.0-6.0. A compound microbial agent is added for a second fermentation. The compound microbial agent is composed of *Pseudomonas aeruginosa* and *Galactosaccharomyces galactosomal*-like bacteria, and the inoculation weight ratio of *Pseudomonas aeruginosa* to *Galactosaccharomyces galactosomal*-like bacteria is 2-4:3-6. Ultrasound is performed once every 3 hours during fermentation, each time for 10-30 minutes, to obtain the second fermentation broth.

[0040] S4. The second fermentation broth obtained in S3 is inactivated and filtered, and then centrifuged at 2000-6000 rpm for 30-60 min to obtain the original fermentation broth of peony flowers.

[0041] (2) The peony flower extract fermentation liquid prepared in (1) is combined with vitamin E and other excipients to obtain a vitamin E moisturizing lotion containing peony flower extract.

[0042] The beneficial effects of this invention are as follows:

[0043] (1) Based on the different types and contents of small and medium molecular components contained in the petals of peony flowers at different growth and development stages, the petals of "Fengdan" peony in the round bud stage were selected as raw materials. At this stage, the flavonoid content in the petals of "Fengdan" peony reached 528.15 U / g.

[0044] (2) In this invention, different types of microbial agents such as Bifida Ferment Lysate, Pseudomonas alterniflora and Galactomyces are used in sequence to ferment the peony flower extract, which significantly improves the skin irritation of the peony flower extract and enhances the moisturizing and skin-nourishing properties of the product, avoiding the occurrence of allergies, itching, and roughness and cracks on the face when the peony flower extract is directly compounded with vitamin E lotion;

[0045] (3) The product prepared by combining the fermentation liquid of peony flowers with vitamin E in this invention has a good anti-allergic effect. The product has an inhibition rate of more than 80% on hyaluronidase activity. Attached Figure Description

[0046] Figure 1 This is a graph showing the changes in skin moisture content of subjects under different usage times for the product of Example 1 of the present invention;

[0047] Figure 2 This is a graph showing the changes in transdermal water loss in subjects at different usage times for the product of Example 1 of the present invention. Detailed Implementation

[0048] To enable those skilled in the art to better understand the present invention, the present invention will now be further described in conjunction with specific embodiments.

[0049] Example 1

[0050] A vitamin E moisturizing lotion with added peony flower extract is prepared as follows:

[0051] (1) Preparation of peony flower raw liquid fermentation broth;

[0052] S1. Select 'Fengdan' peony buds with a diameter of 2.5-5.5cm at the round bud stage, wash them, and physically press them to obtain the original juice of fresh peony flowers. No external water is added during the physical pressing process.

[0053] S2. Sterilize the peony flower extract obtained in S1, adjust the pH to 6.5 at 37℃, add 0.12% of Bifida Ferment Lysate by weight of the peony flower extract for the first fermentation, with the enzyme activity of Bifida Ferment Lysate ≥80,000 U / g, ferment for 10 hours, sterilize and filter after fermentation to obtain the first fermentation liquid.

[0054] S3, the first fermentation broth obtained in S2 is ultrasonically treated at 28℃ with a frequency of 100kHz for 3 minutes, then the temperature is adjusted to 40℃ and the pH is 5.0. A compound microbial agent is added for a second fermentation. The compound microbial agent consists of *Pseudomonas aeruginosa* and *Galactosacchariformis*. The weight of *Pseudomonas aeruginosa* added accounts for 0.1% of the weight of the first fermentation broth, with an enzyme activity ≥70,000 U / g. The weight of *Galactosacchariformis* added accounts for 0.17% of the weight of the first fermentation broth, with an enzyme activity ≥80,000 U / g. That is, the inoculation weight ratio of *Pseudomonas aeruginosa* to *Galactosacchariformis* is 3:5. Ultrasonic treatment is performed once every 3 hours for 25 minutes each time during fermentation to obtain the second fermentation broth.

[0055] S4. The second fermentation broth obtained in S3 is inactivated and filtered, and then centrifuged at 3000 rpm for 40 min to obtain the original fermentation broth of peony flowers.

[0056] (2) The peony flower extract fermentation liquid prepared in (1) is combined with vitamin E and other excipients to obtain vitamin E moisturizing lotion containing peony flower extract.

[0057] The composition, by weight, includes: 6 parts peony flower fermentation liquid, 1 part vitamin E, 5 parts glycerin, 0.5 parts hydroxyethyl urea, 3 parts nicotinamide, 2 parts polydimethylsiloxane, 2 parts peony seed oil, 2 parts hydroxyethylpiperazine ethane sulfonic acid, 1.5 parts cetearyl alcohol, 0.25 parts propylene glycol, 0.05 parts eucommia bark extract, 0.05 parts golden chamomile extract, 0.6 parts 1,2-hexanediol, and honeysuckle. Flower extract 0.01 parts, Panax notoginseng root extract 0.01 parts, inositol 1 part, phytosterol oleate 1 part, glyceryl stearate 0.3 parts, sodium stearoyl lactylate 0.18 parts, 3-O-ethyl ascorbic acid 0.5 parts, p-hydroxyacetophenone 0.5 parts, polyacrylamide dimethyl taurine 0.5 parts, xanthan gum 0.1 parts, carbomer 0.1 parts, sodium hyaluronate 0.02 parts, water to 100 parts.

[0058] In this invention, the content of each component in the "Fengdan" peony at different stages was detected. The specific content of each component in the peony petals at different stages is shown in Table 1.

[0059] Table 1. Content of various components in "Fengdan" peony at different stages

[0060] Flowering period Total phenols (mg / g FW) Flavonoids (U / g FW) Anthocyanins (U / g FW) Budding stage 107.51±3.2a 369.30±2.8b 25.62±1.3cd Little Wind Chimes Period 82.32±1.4b 401.13±25.0b 30.23±0.5c Big Wind Chime Period 71.73±2.5c 489.20±14.8a 39.01±0.6c Bud stage 69.76±4.4c 528.15±4.4b 56.33±3.3a Flowering period 66.28±5.3c 398.23±19.8a 50.56±1.6ab

[0061] Note: Different letters represent differences at the 0.05 level.

[0062] As can be clearly seen from Table 1, for the "Fengdan" peony, the content of active ingredients such as total phenols, flavonoids, and anthocyanins varies significantly at different growth stages. In particular, the flavonoid content in the petals is the highest during the bud stage, reaching 528.15 U / g FW, and the anthocyanin content is 56.33 U / g FW. The flavonoid and anthocyanin contents in the petals at other growth stages are much lower than those during the bud stage.

[0063] In addition, the performance of the product prepared in Example 1 was tested using the following method in this invention:

[0064] Sample usage instructions: Apply the product to the face, covering the entire face, once in the morning and once in the evening, for a period of 4 weeks ± 3 days.

[0065] Subject information: 26 subjects were female and 7 were male; subjects were aged 18 to 60 years; subjects had dry skin and were prone to facial redness.

[0066] The products prepared in this embodiment were tested using the following standards:

[0067] QBT4256-2011 Guidelines for Evaluating the Moisturizing Efficacy of Cosmetics;

[0068] "T-ZHCA003-2018 Test Method for Cosmetics' Influence on Transepidermal Moisture Loss";

[0069] The test temperature is 20-22℃, the relative humidity is 40-60%RH, and other conditions are performed in accordance with the standard requirements.

[0070] Hyaluronidase inhibition rate (sample diluted to 10% aqueous solution) was measured according to the following methods: Wang Ling. Preparation process, efficacy and mechanism of action of anti-allergic and antipruritic plant combination extracts [D]. Harbin: Northeast Agricultural University, 2015; Liu Chao. Extraction and separation of irisin and its role in hyaluronidase [D]. Wuxi: Jiangnan University, 2012.

[0071] The test temperature is 18–26℃, the relative humidity is 45–65%RH, and other conditions are performed in accordance with the standard requirements.

[0072] After using the product of this embodiment for 14 to 28 days, the descriptive statistics of skin stratum corneum moisture content and the changes in stratum corneum moisture content are shown in Tables 2 and 3 below, and attached... Figure 1 As shown, the difference in moisture content is illustrated in Table 4, and the transdermal moisture loss is shown in Tables 5-6 and appendix. Figure 2 .

[0073] Among them, for the moisture content of the stratum corneum, the higher the measured value, the higher the moisture content of the stratum corneum; when measuring transepidermal water loss, the higher the measured value, the more transepidermal water loss.

[0074] Table 2. Descriptive statistics of skin stratum corneum moisture content (n=30)

[0075]

[0076] Table 3. Moisture content of the stratum corneum of the skin

[0077]

[0078] Table 4. Analysis of differences in moisture content in the stratum corneum of the skin.

[0079]

[0080] As can be seen from the table above, after 14 days of product use, the moisture content of the skin's stratum corneum increased by 59.99%, and after 28 days of product use, compared to the initial state, the moisture content of the skin's stratum corneum increased by 67.79%.

[0081] Table 5. Descriptive statistics of transdermal water loss (n=30)

[0082]

[0083]

[0084] Table 6. Analysis of differences in transdermal water loss (comparison before and after sample use)

[0085]

[0086] The test results above show that after 14 days of use, the stratum corneum moisture content of the skin of the 30 subjects increased by 59.99%, and the transepidermal water loss decreased by 28.13%. After 28 days of use, compared with the initial state, the stratum corneum moisture content of the skin of the 30 subjects increased by 67.79%, and the transepidermal water loss decreased by 30.65%. The sample showed good moisturizing properties and the effect of improving skin barrier repair.

[0087] In addition, the anti-allergic properties of the product prepared in Example 1 were tested, and its inhibition rate against hyaluronidase was determined.

[0088] Since hyaluronidase is involved in allergic reactions and is strongly correlated with histamine release from mast cells, the anti-allergic activity of a substance can be determined by the inhibition rate of hyaluronidase activity. The higher the inhibition rate of hyaluronidase activity, the stronger the anti-allergic effect of the substance, and vice versa.

[0089] After testing the product of Example 1 of this invention, its inhibition rate against hyaluronidase was 80.221 ± 8.124%.

[0090] It is evident that the moisturizing lotion prepared by this invention not only has significantly good moisturizing properties, but also, after the peony extract in the product is processed, the overall anti-allergic effect of the product on the skin is significantly improved.

[0091] Example 2

[0092] (1) Preparation of peony flower raw liquid fermentation broth;

[0093] S1. Select 'Fengdan' peony buds with a diameter of about 3.0cm during the round peach stage, wash them, and physically press them to obtain the original juice of fresh peony flowers. No external water is added during the physical pressing process.

[0094] S2, sterilize the peony flower extract obtained in S1, adjust the pH to 6.2 at 35℃, add 0.12% of Bifida ferment lysate by weight of the peony flower extract for the first fermentation, ferment for 12 hours, sterilize and filter after fermentation to obtain the first fermentation liquid;

[0095] S3, the first fermentation broth obtained in S2 is treated with ultrasound at 32°C and 80kHz for 2 minutes, then the temperature is adjusted to 37°C and the pH is 4.2. A compound microbial agent is added for a second fermentation. The compound microbial agent is composed of Pseudomonas aeruginosa and Galactomyces-like bacteria, and the inoculation weight ratio of Pseudomonas aeruginosa to Galactomyces-like bacteria is 2:6. Ultrasound is performed once every 3 hours for 25 minutes each time during the fermentation to obtain the second fermentation broth.

[0096] S4. The second fermentation broth obtained in S3 is inactivated and filtered, and then centrifuged at 3000 rpm for 40 min to obtain the original fermentation broth of peony flowers.

[0097] (2) The peony flower extract fermentation liquid prepared in (1) is combined with vitamin E and other auxiliary ingredients to obtain vitamin E moisturizing lotion containing peony flower extract.

[0098] In this case, by weight, there are 3 parts of peony flower original fermentation liquid and 1 part of vitamin E. The amount of other auxiliary materials added is the same as in Example 1. Then, water is added to make up to 100 parts.

[0099] Example 3

[0100] Unlike Example 1, the inoculation ratio of the compound microbial agent was adjusted only during the second fermentation, wherein the inoculation weight ratio of Pseudomonas alterniflora to Galactomyces-like bacteria was 4:6.

[0101] Example 4

[0102] Unlike Example 1, the inoculation ratio of the compound microbial agent was adjusted only during the second fermentation, wherein the inoculation weight ratio of Pseudomonas alterniflora to Galactomyces-like bacteria was 4:4.

[0103] Example 5

[0104] Unlike Example 1, the inoculation ratio of the compound microbial agent was adjusted only during the second fermentation, wherein the inoculation weight ratio of Pseudomonas alterniflora to Galactomyces-like bacteria was 4:3.

[0105] The results of using the products prepared in Examples 2-5, and the stratum corneum moisture content and transepidermal water loss of the subjects are shown in Table 7 below.

[0106] Table 7 Performance of the products prepared in Examples 2-5

[0107]

[0108] The data in Table 7 show that the product prepared under the fermentation ratio in Example 3 has the best moisturizing effect. After a period of use, the water content of the stratum corneum is the highest. Compared with Examples 2 to 5, it can be seen that during the secondary fermentation, as the inoculation weight ratio of Pseudomonas aeruginosa to Galactomyces-like bacteria gradually decreases, the water retention effect of the vitamin E moisturizing lotion containing peony flower extract fermentation liquid shows a trend of gradually increasing to a maximum value and then decreasing. When the inoculation weight ratio of Pseudomonas aeruginosa to Galactomyces-like bacteria is around 1.5, the compound effect of the obtained fermentation liquid is the best.

[0109] In addition, the products prepared in Examples 2 to 5 all showed a certain inhibitory effect on hyaluronidase. However, due to the different ratios of Pseudomonas alterniflora and Galactomyces in the compound microbial agent added during the secondary fermentation process, the anti-allergic effects of the obtained products varied significantly, but none were as good as the anti-allergic effect of the product obtained under the conditions of Example 1, which had an inhibition rate of up to 80.22% on hyaluronidase. The product showed good anti-allergic and moisturizing properties.

[0110] Comparative Example 1

[0111] The only difference from Example 1 is that the "Fengdan" peony is replaced with the petals of the "Xiangyu" variety peony, and all other operations are the same as in Example 1; the performance of the obtained product is tested, and the testing methods are the same as in Example 1.

[0112] Comparative Example 2

[0113] Unlike Example 1, during the first fermentation, the weight of Bifida Ferment Lysate added accounted for 3% of the weight of the original peony flower extract.

[0114] Comparative Example 3

[0115] Unlike Example 1, only bifida ferment lysate was added for a single fermentation, and no secondary fermentation was performed.

[0116] Comparative Example 4

[0117] Unlike Example 1, instead of using Bifida Ferment Lysate for fermentation, Pseudomonas alterniflora and Galactomyces-like bacteria were added directly to the peony flower extract at a weight ratio of 3:5 for fermentation. All other operations were the same as in Example 1.

[0118] Comparative Example 5

[0119] Unlike Example 1, the obtained peony flower extract was not fermented, but directly compounded with vitamin E and other excipients according to the proportions in Example 1.

[0120] Table 8 shows the moisture content of the stratum corneum of the skin before and after use of the products prepared in Comparative Examples 1 to 5, as well as the inhibition rate of the products on hyaluronic acid.

[0121] Table 8. Performance of the products prepared in Comparative Examples 1–5

[0122]

[0123] The data in Table 8 show that, in Comparative Example 1, the experimental results of using the fresh flower extract of the classic traditional peony variety "Xiangyu" showed no significant difference in the change of stratum corneum moisture content and the inhibition rate of hyaluronidase compared to "Fengdan" peony after a period of use. However, "Xiangyu" peony is an ornamental variety, and using the petals of this variety as raw material would undoubtedly greatly increase the production cost of the product. In contrast, the "Fengdan" peony used in this invention is the most widely planted variety, and its raw material is inexpensive and more suitable for actual production.

[0124] Furthermore, data from Comparative Examples 2-5 show that changing the fermentation process and the proportion of added microorganisms in this invention resulted in a significantly lower effect of the obtained moisturizing lotion compared to Example 1.

[0125] In Comparative Example 2, the amount of Bifida Ferment Lysate added during the first fermentation was relatively high, which may have inhibited the reproduction of the Bifida Ferment Lysate community to a certain extent, resulting in insufficient fermentation during the first fermentation and thus affecting the performance of the final product.

[0126] In Comparative Examples 3 and 4, all underwent a single fermentation. Clearly, the resulting products had lower stratum corneum moisture content, poorer moisturizing effects, and lower inhibition rates of hyaluronidase, ranging from only 39.12% to 40.78%. This demonstrates that double fermentation allows for a more complete extraction of active substances from the "Fengdan" peony petals, and under the action of microorganisms, these substances can be better converted into active ingredients that the skin can absorb, thus better maintaining skin moisture and preventing moisture loss. It also avoids the strong irritation to the skin caused by the components in the peony petal extract.

[0127] In Comparative Example 5, the product prepared by directly using peony flower extract showed poor hyaluronidase inhibition rate and moisturizing effect.

[0128] Considering the data from the above product efficacy tests, as well as the economic and social benefits such as raw material costs, choosing "Fengdan" peony petals as the raw material for the product design of this invention is undoubtedly the best option at present.

Claims

1. A vitamin E moisturizing lotion containing peony flower extract, characterized in that, By weight, it includes 1 to 10 parts of peony flower raw material fermentation liquid and 0.1 to 2 parts of vitamin E; The peony flower extract fermentation liquid is obtained by fermenting peony flower extract twice. The microbial agent used in the first fermentation is Bifida ferment lysate, and the microbial agent used in the second fermentation is a compound microbial agent, including Pseudomonas aeruginosa and Galactomyces-like bacteria. The peony mentioned is the "Fengdan" peony in its bud stage; During the first fermentation, the weight of Bifida Ferment Lysate added accounted for 0.12% of the weight of the original peony flower extract, and the enzyme activity of Bifida Ferment Lysate was ≥80,000 U / g; In the microbial agent used during the second fermentation, the inoculation weight ratio of *Pseudomonas aeruginosa* to *Galactomyces* was 3:

5.

2. The vitamin E moisturizing lotion with added peony flower extract as described in claim 1, characterized in that, The moisturizing lotion also includes the following ingredients: 1-10 parts glycerin, 0.5-2 parts hydroxyethyl urea, 1-5 parts niacinamide, 1-5 parts dimethicone, 0.5-3 parts peony seed oil, 0.5-5 parts hydroxyethylpiperazine ethane sulfonic acid, 0.5-2 parts cetearyl alcohol, 0.1-0.5 parts propylene glycol, 0.01-0.1 parts eucommia bark extract, 0.01-0.1 parts golden chamomile extract, 0.1-1 parts 1,2-hexanediol, 0.01-0.05 parts honeysuckle flower extract, and Panax notoginseng root. Extract 0.01~0.05 parts, inositol 0.2~2 parts, phytosterol oleate 0.5~2 parts, glyceryl stearate 0.1~0.5 parts, sodium stearoyl lactylate 0.1~0.5 parts, 3-O-ethyl ascorbic acid 0.1~1 parts, p-hydroxyacetophenone 0.1~1 parts, polyacrylamide dimethyl taurate 0.1~1 parts, xanthan gum 0.05~0.5 parts, carbomer 0.05~0.5 parts, sodium hyaluronate 0.01~0.05 parts, water to make up to 100 parts, all of the above are parts by weight.

3. The method for preparing the vitamin E moisturizing lotion with added peony flower extract as described in claim 1, characterized in that, The steps include the following: (1) Preparation of peony flower raw material fermentation broth; S1. Select peony buds at the round bud stage of "Fengdan" peony, wash them, and physically press them to obtain peony flower extract. S2, sterilize the peony flower extract obtained in S1, then add bifida ferment lysate for the first fermentation, sterilize and filter after fermentation to obtain the first fermentation liquid; S3, the first fermentation broth obtained in S2 is subjected to ultrasonic treatment, and then a compound microbial agent is added for a second fermentation. The compound microbial agent is composed of Pseudomonas alterniflora and Galactomyces-like bacteria. During the fermentation, ultrasonic treatment is performed once every 3 hours for 10-30 minutes each time to obtain the second fermentation broth. S4. The second fermentation broth obtained in S3 is inactivated, filtered, and centrifuged to obtain the original fermentation broth of peony flowers. (2) The peony flower extract fermentation liquid prepared in (1) is combined with vitamin E and other excipients to obtain a vitamin E moisturizing lotion with added peony flower extract.

4. The method for adding peony flower extract to a vitamin E moisturizing lotion as described in claim 3, characterized in that, The peony buds described in S1 have a diameter of 2.5 to 5.5 cm.

5. The method for adding peony flower extract to a vitamin E moisturizing lotion as described in claim 3, characterized in that, In S2, the temperature of the first fermentation is 36~40℃, the pH is 6.0~7.0, and the fermentation time is 10~12 h.

6. The method for adding peony flower extract to a vitamin E moisturizing lotion as described in claim 3, characterized in that, In S3, the temperature of the second fermentation is 32~40℃, the pH is 4.0~6.0, and the fermentation time is 36~72 h.

7. The method for adding peony flower extract to a vitamin E moisturizing lotion as described in claim 3, characterized in that, The ultrasonic treatment described in S3 specifically involves ultrasonically treating the first fermentation broth obtained in S2 at 25~40℃ and a frequency of 80~120 kHz for 2~3 minutes.

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