Application of methyl jasmonate in improving nitrogen use efficiency of plants
By applying an appropriate concentration of exogenous methyl jasmonate to the apple tree cultivation system, the problem of inhibited growth of apple trees under limited nitrogen supply was solved, biomass, root growth and photosynthetic capacity were improved, nitrogen use efficiency was enhanced, and environmental pollution from fertilizer use was reduced.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NORTHWEST A & F UNIV
- Filing Date
- 2024-03-25
- Publication Date
- 2026-04-21
AI Technical Summary
Under limited nitrogen supply conditions, apple trees have low nitrogen use efficiency, which leads to inhibited growth, and excessive nitrogen fertilizer in the soil causes environmental pollution. Existing technologies have not been able to effectively address the impact of exogenous methyl jasmonate on fruit tree growth.
Exogenous methyl jasmonate was applied to the cultivation system for plant growth at a concentration of 0.5–10 μmol/L, preferably 1–2.5 μmol/L, to improve biomass accumulation and root growth of plants under limited nitrogen supply, enhance photosynthetic capacity, and regulate plant nitrogen use efficiency.
It significantly improved the biomass, root growth, and photosynthetic capacity of apple trees, reduced the carbon-to-nitrogen ratio, improved nitrogen use efficiency, alleviated growth inhibition caused by nitrogen starvation, saved fertilizer costs, and reduced environmental pollution.
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Figure CN118044514B_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of fruit tree cultivation technology, specifically relating to the application of methyl jasmonate in improving the nitrogen use efficiency of plants. Background Technology
[0002] Nitrogen, as the main nutrient element for the growth and development of fruit trees, is obtained by absorbing NO from the soil through the roots. 3- NH 4+ Nitrogen, along with simple organic nitrogen, participates in various life activities related to apple growth and development. Nitrogen metabolism affects the total nitrogen accumulation, protein content, and enzyme synthesis within the plant. When plants suffer from nitrogen starvation, their ability to absorb and convert nitrogen decreases, affecting the synthesis of chlorophyll precursors and chlorophyll itself. Chlorophyll is a crucial factor essential for photosynthesis, leading to a decrease in the photosynthetic rate, reduced leaf dry matter accumulation, and inhibited above-ground growth. Plants absorb nutrients from the soil through their roots. Under nutrient stress, the root system structure changes. Nitrogen starvation promotes the growth and development of total root length, total root surface area, average root diameter, total root volume, and the number of lateral roots, allowing for better nitrogen absorption.
[0003] Apples are one of my country's important economic fruit trees. In recent years, my country's apple industry has developed steadily, with the Loess Plateau region accounting for approximately 58% of the national apple planting area and 52% of the national apple production. However, the soil fertility in this region is low, and the large amounts of nitrogen fertilizer applied to increase apple yields have led to a serious excess of nitrates in the soil. Therefore, improving the nitrogen use efficiency (NUE) of apples is an important approach and strategy for maintaining the healthy and sustainable development of my country's apple industry.
[0004] Jasmonic acid (JA) is an endogenous growth regulator found in higher plants. It plays a crucial physiological regulatory role in plant growth and development, actively responding to biotic and abiotic stresses. Jasmonic acid, along with its volatile methyl ester derivative methyl jasmonate (MeJA), amino acid derivatives (such as JA-Ile), and the active precursor 12-oxo-phytodienoic acid (OPDA), are collectively known as jasmonic acids (JAs). Jasmonic acids participate in regulating plant growth and development and responses to external stresses. In plant growth and development, they play important roles in axis elongation during embryogenesis, flower development during reproductive growth, leaf senescence during growth, root formation, lateral root development, and stomatal opening. In response to nutrient stress, the jasmonic acid signaling pathway can sense nutrient stress and regulate plant responses by increasing or inhibiting the expression of jasmonic acid-related genes.
[0005] However, no research has been reported on the effects of exogenous methyl jasmonate on fruit tree growth under limited nitrogen supply. Summary of the Invention
[0006] To address the aforementioned problems, this invention provides an application of methyl jasmonic acid in improving nitrogen use efficiency in plants. This invention analyzes the effects of exogenous methyl jasmonic acid on the growth of Pingyi sweet tea under limited nitrogen supply, proposes suitable methyl jasmonic acid application methods, enriches the regulatory mechanism of nitrogen use efficiency in apples under limited nitrogen supply, and lays a theoretical foundation for the development of cultivation techniques that rely on the jasmonic acid pathway to improve nitrogen use efficiency in apples.
[0007] To achieve the above objectives, the technical solution provided by the present invention is as follows:
[0008] This invention provides an application of methyl jasmonate in improving nitrogen use efficiency in plants.
[0009] Preferably, in application, exogenous methyl jasmonate is applied to the plant growth cultivation system to a final concentration of 0.5–10 μmol / L.
[0010] Preferably, exogenous methyl jasmonate is applied to the cultivation system for plant growth until its final concentration is 1–2.5 μmol / L.
[0011] Preferably, exogenous methyl jasmonate is applied to the cultivation system for plant growth to a final concentration of 2.5 μmol / L.
[0012] Preferably, the methyl jasmonate is used to improve the accumulation of plant biomass or the growth and development of roots under limited nitrogen supply.
[0013] Preferably, the limited nitrogen refers to a nitrogen content in the plant cultivation system that is not higher than 0.2 mmol / L.
[0014] Preferably, the accumulation of biomass is the accumulation of plant fresh weight or dry weight.
[0015] Preferably, improving the growth and development of jasmine roots involves increasing the total root length, total root surface area, total root volume, number of root tips, number of lateral roots, or root vitality.
[0016] Preferably, the methyl jasmonate is used to reduce the carbon-to-nitrogen ratio of plants or to increase the nitrogen content of plants.
[0017] Preferably, the methyl jasmonate is used to improve the photosynthetic capacity of plants.
[0018] Preferably, improving plant photosynthetic capacity involves increasing the plant's chlorophyll content, net photosynthetic rate, intercellular carbon dioxide concentration, or stomatal conductance.
[0019] Preferably, the plant is an apple.
[0020] Preferably, the apple is Pingyi sweet tea.
[0021] Compared with the prior art, the present invention has the following beneficial effects:
[0022] 1. This invention uses Pingyi sweet tea, a species of the genus Malus with apomixis, as material. By analyzing the effect of exogenous methyl jasmonate on the growth of Pingyi sweet tea under limited nitrogen supply, it shows that exogenous methyl jasmonate can improve the nitrogen use efficiency of plants and effectively alleviate the growth inhibition of Pingyi sweet tea caused by nitrogen starvation. Specifically, after applying methyl jasmonate, the biomass of the plants increases, the root system grows better, the photosynthetic capacity is enhanced, the nitrogen content in the plant increases, the carbon-nitrogen ratio decreases, and the nitrogen use efficiency is improved.
[0023] 2. This invention treated Pingyi sweet tea seedlings cultured in hydroponics under low nitrogen conditions with methyl jasmonate at concentrations of 1–2.5 μmol / L. The results showed that methyl jasmonate treatment improved nitrogen use efficiency and alleviated the inhibitory effect of nitrogen starvation on plant growth, manifested in significant increases in fresh weight, dry weight, total root length, total root surface area, total root volume, number of root tips, number of lateral roots, and root activity. Simultaneously, exogenous methyl jasmonate treatment significantly increased chlorophyll content, maximum photochemical efficiency (Fv / Fm), net photosynthetic rate, nitrogen content, and reduced carbon-to-nitrogen ratio, thus improving nitrogen use efficiency. This indicates that appropriate concentrations of methyl jasmonate can significantly improve nitrogen use efficiency in plants, alleviate the inhibitory effect of nitrogen starvation on apple plant growth, further enrich the regulatory mechanism of nitrogen use efficiency in apples under limited nitrogen supply, and lay a theoretical foundation for developing cultivation techniques that rely on the jasmonate pathway to improve nitrogen use efficiency in apples.
[0024] 3. The method of the present invention is simple and easy to operate. The amount of methyl jasmonate used is only 1 to 2.5 μmol / L, which is a small dosage and can save fertilizer costs and alleviate environmental pollution caused by excessive use of fertilizers. Attached Figure Description
[0025] Figure 1 This is the growth phenotype of Pingyi sweet tea plants after 30 days of treatment according to Example 1 of the present invention.
[0026] Figure 2 The data in Example 1 of this invention are the fresh weight (a), dry weight (b), total root length (c), total root surface area (d), total root volume (e), number of root tips (f), number of lateral roots (g), and root activity (h) of Pingyi sweet tea plants treated for 30 days.
[0027] Figure 3This invention describes the detection of various indicators of the photosynthetic system of Pingyi sweet plants treated for 0-30 days in Example 2 of this invention, including SPAD value (a), maximum photochemical efficiency Fv / Fm (b), net photosynthetic rate (c), intercellular carbon dioxide concentration (d), and stomatal conductance (e).
[0028] Figure 4 The nitrogen content (a), carbon content (b), and carbon-nitrogen ratio (c) of Pingyi sweet potato plants treated for 30 days in Example 3 of this invention are shown. 15 The percentage of nitrogen atoms (d) and nitrogen use efficiency (e). Detailed Implementation
[0029] The present invention will be further described in detail below with reference to embodiments, so that those skilled in the art can implement it based on the description. The specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
[0030] Unless otherwise specified, the experimental and detection methods described in the following embodiments are conventional methods; the reagents and materials described are commercially available unless otherwise specified; and the index data described are conventional measurement methods unless otherwise specified.
[0031] The methyl jasmonate described in this invention is an organic compound with the molecular formula C0. 13 H 20 O3, with a molecular weight of 224.30 and a purity of 98%, is generally diluted with anhydrous ethanol when preparing this product.
[0032] Preparation of reagents used in the embodiments of the present invention
[0033] (1) 1 / 2 Hoagland nutrient solution: composed of solution A, solution B, and solution C. Solution A contains 0.41 g / L Ca(NO3)2·4H2O and 0.253 g / L KNO3; solution B contains 0.247 g / L MgSO4·7H2O and 0.068 g / L KH2PO4·2H2O; solution C contains 18.355 mg / L Fe-Na-EDTA, 1.430 mg / L H3BO3, 0.110 mg / L ZnSO4·7H2O, 0.905 mg / L MnCl2·4H2O, 0.040 mg / L CuSO4·5H2O, and 0.045 mg / L H2MoO4·H2O. The nitrogen content in the 1 / 2 Hoagland nutrient solution is 6 mmol / L after conversion.
[0034] (2) 100mM methyl jasmonate stock solution: 0.212ml of 98% methyl jasmonate was diluted to 10ml with anhydrous ethanol. After the methyl jasmonate stock solution was prepared, it should be sealed and stored at room temperature away from light.
[0035] (3) Nitrogen starvation culture medium: The nitrogen content of solution A in 1 / 2 Hoagland nutrient solution is adjusted to 0.2 mmol / L, while solutions B and C have the same composition as 1 / 2 Hoagland nutrient solution.
[0036] (4) Solution A: It is prepared by adding 25 μL of 100 mM methyl jasmonate stock solution to 5 L of nitrogen-starved culture medium. The concentration of methyl jasmonate in the solution is 0.5 μM.
[0037] (5) Solution B: It is prepared by adding 50 μL of 100 mM methyl jasmonate stock solution to 5 L of nitrogen-starved culture medium. The concentration of methyl jasmonate in the solution is 1 μM.
[0038] (6) Solution C: It is prepared by adding 125 μL of 100 mM methyl jasmonate stock solution to 5 L of nitrogen-starved culture medium. The concentration of methyl jasmonate in the solution is 2.5 μM.
[0039] (7) Solution D: It is prepared by adding 250 μL of 100 mM methyl jasmonate stock solution to 5 L of nitrogen-starved culture medium. The concentration of methyl jasmonate in the solution is 5 μM.
[0040] (8) Solution E: It is prepared by adding 500 μL of 100 mM methyl jasmonate stock solution to 5 L of nitrogen-starved culture medium. The concentration of methyl jasmonate in the solution is 10 μM.
[0041] Example 1
[0042] Phenotypic and growth differences in nitrogen starvation response of Pingyi sweet tea after methyl jasmonate application
[0043] Using Pingyi sweet tea, a common rootstock of apple plants, as material, we analyzed the effect of exogenous methyl jasmonate on nitrogen-deficient nutrition (NUE) of Pingyi sweet tea and observed the phenotypic and growth differences of Pingyi sweet tea under nitrogen starvation after methyl jasmonate application.
[0044] 1. Materials and Methods
[0045] The experiment began in May 2023 and was conducted in the Fruit Tree Stress Biology Laboratory of Northwest A&F University in Yangling District, Xianyang City, Shaanxi Province.
[0046] The seeds of Pingyi sweet tea were collected in Pingyi County, Shandong Province, China (35°07'N, 117°25'E). After being stored in sand at 4°C for one month, the seeds were sown in 5×10-cell trays and cultured using a mixed substrate of 3:1:1 volume ratio of substrate, vermiculite, and perlite. When the seedlings grew to 5-6 true leaves, experimental treatments were conducted.
[0047] Uniformly growing Pingyi sweet tea seedlings were selected. After washing the roots, they were fixed to 4×6-hole foam boards using hydroponic cotton and placed in hydroponic trays containing 5L of 1 / 2 Hoagland nutrient solution (the pH was adjusted to 5.8–6.0 using phosphoric acid). Intermittent aeration was provided using an air pump, maintaining a dissolved oxygen concentration of 8.0–8.5 mg / L. The nutrient solution was changed every five days. The hydroponic temperature was 23–25℃, the photoperiod was 14h light / 8h darkness, and the light intensity was 160 μmol / m². -2 s -1 Subsequent treatment should be carried out under these conditions for 15 days until the seedlings have grown new roots and leaves.
[0048] Methyl jasmonate (MeJA) was used as an exogenous hormone and applied to different nutrient solutions for 30 days. The experiment consisted of seven treatments, with 72 Pingyi sweet tea seedlings treated according to the experimental protocol in Table 1. Each treatment used 72 Pingyi sweet tea plants (24 plants per replicate, 3 replicates). After 30 days of treatment, the hydroponic Pingyi sweet tea seedlings were cleaned, dried, and their fresh weight was measured. They were then blanched at 105℃ for 30 minutes and dried at 65℃ until constant weight, and their dry weight was measured. After 30 days of nitrogen starvation treatment, intact fresh plant roots were collected, washed with deionized water, and scanned using a SNAPSCAN 310 scanner (Seiko Epson Corp., Bangalore, India). Root architecture parameters, including total root length, total root surface area, total root volume, number of root tips, and number of lateral roots, were analyzed using a WinRhizo image analysis system (V4.1 c; Regent Instruments, Quebec City, Quebec, Canada). Root activity was measured using a root activity / dehydrogenase (DHA) kit from Suzhou Keming Biotechnology Co., Ltd.
[0049] Table 1 Hydroponic Treatment Scheme
[0050] hydroponic system Process one group (CK) 1 / 2 Hoagland nutrient solution Treatment group 2 (0LN) Nitrogen starvation culture medium Three groups (0.5 LN) were treated. Solution A Four groups (1LN) were treated. Solution B Five groups (2.5 LN) were treated. Solution C Six groups (5LN) were treated. Solution D Seven groups (10 LN) were treated. Solution E
[0051] 2. Test Results
[0052] like Figure 1 As shown, after 30 days of nitrogen starvation treatment, the growth of treatment group 2 (0LN) and treatment group 7 (10LN) was relatively worse than that of other treatment groups, specifically in that the plants were stunted and the leaves were narrow and slightly yellow.
[0053] In addition, such as Figure 2As shown in a to b, after nitrogen starvation treatment, the fresh weight and dry weight of the plants were significantly reduced. After measuring relevant indicators, it was found that the fresh weight and dry weight of the plants after applying exogenous methyl jasmonate were higher than those of treatment group 2 (0LN). Among them, the fresh weight and dry weight of Pingyi sweet tea in treatment group 5 (2.5LN) with 2.5μM exogenous methyl jasmonate were the highest, which were significantly higher than those of treatment group 2 (0LN).
[0054] Under nitrogen starvation treatment (OLN), the root architecture of plants underwent significant changes. Total root length, total root surface area, total root volume, and the number of root tips and lateral roots increased significantly, by 14.48%, 9.3%, 37.05%, 14.47%, and 3.26%, respectively, compared to the control group (CK). Root activity was significantly reduced, decreasing by 37.5% compared to the control group (CK). After treatment with exogenous methyl jasmonate, the total root length, total root surface area, total root volume, and the number of root tips and lateral roots increased even more significantly. Figure 2 c~h). Among all treatments involving the application of exogenous methyl jasmonate, the five groups treated with 2.5 μM exogenous methyl jasmonate (2.5 LN) showed the best root growth in Pingyi sweet tea. Compared with treatment group 1 (CK), the total root length, total root surface area, total root volume, and the number of root tips and lateral roots increased by 35.23%, 60.6%, 104.9%, 79.2%, and 50.39%, respectively; root activity was also significantly enhanced, increasing by 40% compared with treatment group 2 (0 LN). Figure 2 c~h).
[0055] The above results indicate that exogenous methyl jasmonate can increase the biomass accumulation of Pingyi sweet tea under nitrogen starvation, promote root growth of Pingyi sweet tea, and reduce the growth differences caused by nitrogen starvation.
[0056] Example 2
[0057] Comparison of photosynthetic capacity of Pingyi sweet tea after application of methyl jasmonate under nitrogen starvation
[0058] Using Pingyi sweet tea, a common rootstock of apple plants, as material, this study analyzed the effect of exogenous methyl jasmonate on the nitrogen utilization efficiency (NUE) of Pingyi sweet tea and compared the photosynthetic capacity of Pingyi sweet tea after applying methyl jasmonate under nitrogen starvation.
[0059] 1. Materials and Methods
[0060] Pingyi sweet tea plants were treated according to the method in Example 1. The maximum photochemical efficiency (Fv / Fm) of PSII was measured using a PAM2500 chlorophyll fluorometer. Leaves were dark-acclimated for 15 minutes before measurement. The relative chlorophyll content (SPAD value) was measured using a SPAD-502Pluse every 5 days from treatment day 0 to 30. A CIRAS-3 portable photosynthesis meter was used to measure photosynthetic parameters such as net photosynthetic rate (Pn), intercellular carbon dioxide concentration (Ci), and transpiration rate (Tr) of mature leaves with sufficient light and consistent leaf position from each treatment group between 9:00 and 11:00 AM using a CIRAS-3 portable photosynthesis meter. Measurements were taken on days 0, 5, 10, 20, and 30 of treatment.
[0061] 2. Test Results
[0062] Nitrogen starvation treatment resulted in lower chlorophyll content in plant leaves compared to the control, and the difference between the two treatments gradually increased with the duration of nitrogen starvation. After 30 days of treatment, the chlorophyll content in treatment group 2 (0LN) was the lowest and significantly lower than that in the treatment with methyl jasmonate. Specifically, treatment group 5 (2.5LN) with exogenous application of 2.5 μM methyl jasmonate significantly increased the chlorophyll content of the plants. Figure 3 a).
[0063] Consistent with this trend, the maximum photochemical efficiency (Fv / Fm) of plants decreased significantly after nitrogen starvation treatment, but the decrease was less pronounced after applying a certain concentration of exogenous methyl jasmonate. Specifically, the treatment groups (0.5 LN), (1 LN), and (2.5 LN) groups treated with exogenous methyl jasmonate at concentrations of 0.5 μM, 1 μM, and 2.5 μM were essentially no different from treatment group 1 (CK). Figure 3 b).
[0064] Furthermore, the net photosynthetic rate of plants treated with nitrogen starvation (0LN) gradually decreased, and the longer the treatment time, the greater the difference compared with treatment group 1 (CK). After applying an appropriate amount of exogenous methyl jasmonate, the net photosynthetic rate of the plants could be maintained at a high level, with the net photosynthetic rate of treatment group 5 (2.5LN) being basically consistent with that of treatment group 1 (CK). Figure 3 c). Consistent with this trend are intercellular carbon dioxide concentration and stomatal conductance ( Figure 3 d~e).
[0065] The above results indicate that applying an appropriate concentration of exogenous methyl jasmonate can alleviate the effects of nitrogen starvation on plant photosynthetic capacity by increasing photosynthetic-related indicators such as chlorophyll content, with 2.5 μM exogenous methyl jasmonate being the optimal concentration.
[0066] Example 3
[0067] Comparison of nitrogen use efficiency of Pingyi sweet tea after application of methyl jasmonate under nitrogen starvation
[0068] Using Pingyi sweet tea, a common rootstock of apple plants, as material, the effect of exogenous methyl jasmonate on the nitrogen-neutralization (NUE) of Pingyi sweet tea was analyzed. The carbon-nitrogen content, ratio, and NUE of Pingyi sweet tea after application of methyl jasmonate under nitrogen starvation were observed and compared.
[0069] 1. Materials and Methods
[0070] The Pingyi sweet tea plants were treated according to the method in Example 1. After 30 days of treatment, the dry weight was measured, and the sample was ground using a high-throughput grinder. Then, 0.1 g of the sample was accurately weighed and placed in a 100 ml digestion tube, and 5 ml of H2SO4 was added. The digestion apparatus temperature was set to 375°C. After reaching the reaction temperature, the sample was heated for 30 minutes, cooled for 15 minutes, and then 5 ml of 30% H2O2 was added. This process was repeated several times until the solution became clear and transparent. Finally, the volume was adjusted to 100 mL with deionized water. Each treatment was performed in triplicate, and at least two blank controls were included for the same batch. The nitrogen content was determined using an AA3 continuous flow analyzer.
[0071] Weigh 0.1g of the dried sample that has passed through an 80-mesh sieve and determine the carbon content using a carbon-nitrogen analyzer. The carbon-nitrogen ratio is the ratio of plant carbon content to plant nitrogen content.
[0072] Nitrogen use efficiency (NUE): After 30 days of treatment, the nitrogen source in the nutrient solution is compared with that of nitrogen using δ¹⁸O₂. 15 After treatment with N-labeled KNO3 for 3 hours, dried plant samples were collected, and stable isotope δ¹⁴ ions were measured. 15 N percentage and concentration. Elemental analysis was performed using a Flash 2000HT (FlashEA 1112HT, Thermo Fisher Scientific, Inc., USA) elemental analyzer combined with a stable isotope mass spectrometer (Finnigan DELTAV Advantage, Thermo Fisher Scientific, Inc.). 15 The method for calculating nitrogen content is as follows: 15 The nitrogen concentration is the product of the nitrogen content and the dry weight. Nitrogen use efficiency (NUE) is calculated as the product of the total nitrogen content of the apple plant. 15 The ratio of nitrogen content to total fertilizer application.
[0073] 2. Test Results
[0074] The results showed that the nitrogen content of treatment group 2 (0LN) was significantly reduced after nitrogen starvation treatment, decreasing by 26.03% compared with treatment group 1 (CK), while the nitrogen content of treatment group 5 (2.5LN) treated with 2.5 μM exogenous methyl jasmonate decreased by 16.55%. Figure 4 a).
[0075] Nitrogen starvation treatment increased the carbon content of plants, while application of exogenous methyl jasmonate significantly reduced the carbon content. Figure 4 b). The carbon-to-nitrogen ratio (C / N ratio) of plants after nitrogen starvation treatment significantly increased compared to treatment group 1 (CK). Specifically, the C / N ratio of treatment group 2 (0LN) and treatment group 5 (2.5LN) treated with 2.5 μM exogenous methyl jasmonate increased by 35.5% and 19.2%, respectively. Compared to treatment group 2 (0LN), treatment with 2.5 μM exogenous methyl jasmonate significantly reduced the C / N ratio of plants under nitrogen starvation. Figure 4 c).
[0076] After nitrogen starvation treatment, the plants in the two treatment groups (0LN) 15 The percentage of nitrogen atoms decreased significantly compared to the control group (CK), while the application of an appropriate concentration of exogenous methyl jasmonate significantly improved plant health. 15 Percentage of N atoms ( Figure 4 d), of which five groups were treated with 2.5 μM exogenous methyl jasmonate (2.5 LN). 15 The percentage of nitrogen atoms was significantly higher than in other treatment groups. After nitrogen starvation treatment, the application of 1 μM and 2.5 μM exogenous methyl jasmonate increased the nitrogen use efficiency of plants and was significantly higher than that of treatment group two (0LN). Among them, the application of 2.5 μM exogenous methyl jasmonate was optimal, increasing nitrogen use efficiency by 34.78% compared to treatment group two (0LN). Figure 4 e).
[0077] The above results indicate that nitrogen content in plants decreases after nitrogen starvation treatment, and exogenous application of methyl jasmonate can improve nitrogen use efficiency in Pingyi sweet tea by increasing plant nitrogen content and reducing the nitrogen-carbon ratio.
[0078] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. It should be noted that any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention that are of ordinary technical skill should be included within the scope of protection of the present invention.
Claims
1. The application of methyl jasmonate in improving nitrogen use efficiency in plants, characterized in that, The methyl jasmonate is used to improve the accumulation of plant biomass or the growth and development of roots under limited nitrogen supply, wherein limited nitrogen refers to nitrogen content in the plant cultivation system not exceeding 0.2 mmol / L; When applying, exogenous methyl jasmonate is added to the plant cultivation system until the final concentration is 1–2.5 μmol / L.
2. The application according to claim 1, characterized in that, The accumulation of biomass refers to the accumulation of plant fresh or dry weight.
3. The application according to claim 1, characterized in that, Improving the growth and development of jasmine roots involves increasing the total root length, total root surface area, total root volume, number of root tips, number of lateral roots, or root vitality.
4. The application according to claim 1, characterized in that, The methyl jasmonate is used to reduce the carbon-nitrogen ratio of plants or increase the nitrogen content of plants.
5. The application according to claim 1, characterized in that, The methyl jasmonate is used to enhance the photosynthetic capacity of plants.
6. The application according to claim 5, characterized in that, Improving plant photosynthetic capacity involves increasing chlorophyll content, net photosynthetic rate, intercellular carbon dioxide concentration, or stomatal conductance.
7. The application according to claim 1, characterized in that, The plant in question is an apple.