A composition containing nematicide and its use

Abstract

The invention discloses an insecticide synergistic composition containing a piperic acid derivative compound and an application thereof. The composition consists of two active ingredients, compound A and compound B, wherein component B is selected from any one or two of nematicides; the nematicides include thiazolylphos, fluopyram, chloropicrin, dazomet, amino-oligosaccharides, and Paecilomyces lilacinus; the compound I-72 has the following structure: The composition of the invention has the advantages of obvious synergy, delayed resistance, and the like, can be used for preventing and controlling various nematode diseases, and has a good prevention and control effect on various pests such as Lepidoptera, Thysanoptera, Diptera, Hemiptera, etc.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide application, and particularly relates to a composition containing a nematicide and application thereof. Background Art

[0002] In agricultural production practice, the application of chemical agents is still the most effective means of preventing and controlling pests and diseases. However, insecticides containing a single ingredient often have certain limitations in pest control, such as narrow insecticide spectrum, high cost, short duration, and pests are very likely to develop resistance after continuous use. Mixing two insecticides with different mechanisms of action into a mixed component in a certain proportion can overcome the above shortcomings and play a role in improving the efficacy, expanding the insecticide spectrum, and slowing down the development of resistance.

[0003] There are few effective nematicides on the market. Nematicides such as thiazofosinate and fluopyram are mainstream chemical pesticides. They have high insecticidal activity and a wide insecticidal spectrum, making them the most popular and fiercely competitive products in the current pesticide market. However, with the widespread use of thiazofosinate in the prevention and control of nematode diseases, the resistance problem is becoming increasingly serious. Literature reports that southern root-knot nematodes have developed serious resistance to thiazofosinate in some areas, and once resistance is developed, sensitivity is difficult to recover. Therefore, reasonable mixing has become one of the effective means to extend the service life of this variety.

[0004] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity, good conduction activity, and can be used to control a variety of different types of agricultural pests. The compound has a novel structure, a broad insecticide spectrum, high activity, and no cross-resistance with other insecticides, and is expected to become an environmentally friendly agent for the control of resistant pests.

[0005] Mixing pesticides with different mechanisms of action can produce many additional advantages and effects, overcome the shortcomings of using a single pesticide, improve the efficacy, slow down the development of resistance, and more importantly, extend the service life of new varieties in the market and reduce the cost of new pesticide research and development through reasonable mixing of pesticides. Through literature search, no specific technical solution of a binary nematicide composition containing compound I-72 has been disclosed. Summary of the invention

[0006] The present invention aims to develop a composition containing a nematicide and its use.

[0007] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is as follows:

[0008] A composition containing a nematicide, the composition comprises two active components A and B, component A is selected from a compound of the following structure (i.e., compound I-72), and component B is selected from a nematicide; the weight ratio of the two active components A and B is 1:99 to 99:1; wherein the compound structural formula is as follows:

[0009]

[0010] The nematicides are halogenated hydrocarbons, organic sulfurs, other fumigants, organic phosphorus, carbamates, aromatic formamides, trifluorobutenes, oxadiazole sulfides, condensed rings, other non-fumigants or microorganisms.

[0011] The halogenated hydrocarbons include 1,3-dichloropropene, dibromochloropropane (DBCP), dibromoethylene (EDB), tetrachlorothiophene, 1,2-dichloropropane, chloropicrin, dichloroisopropyl ether (DCIP), bromomethane, and methyliodide;

[0012] Organic sulfur: carbon disulfide, sulfuryl fluoride, carbon oxysulfide (COS), methyl isothiocyanate, dimethyl disulfide, diallyl disulfide, methylene dithiocyanate, sodium tetrathiocarbonate, dazomet, and metham-sodium;

[0013] Other fumigants: xylenols, calcium cyanamide, cyanogen, sodium azide, furfural, acrolein, formaldehyde, propylene oxide;

[0014] Organophosphorus: phosphamidon, heterophos, profenofos, phosphocarb, fensulfothion, thionazin, isazofos, dichlofenthion, famphur, dimethoate, terbufos, phorate, cadusafos, ethoprophos, diamidafos, fenamiphos, isofenphos methyl, isofenphos ethyl, isamidofos, fosthietan, fosthiazate, imicyafos, mecarphon;

[0015] Carbamates: alanycarb, aldicarb, aldoxycarb, oxamyl, tirpate, thiodicarb, carbofuran, cloethocarb, benomyl, tirpate;

[0016] Arylformamides: fluopyram, fluopimomide, pydiflumetofen, cyclobutrifluram;

[0017] Trifluorobutylenes: trifluorofuronate (LH517), fluensulfone, trifluenfuronate;

[0018] Oxadiazole sulfide: tioxazafen, oxadiazole sulfide;

[0019] Condensed rings: trifluoroimidazole amide (fluazaindolizine), activated ester (acibenzolar-S-methyl), benclothiaz;

[0020] Other non-fumigation products: ipriodine, carvacrol, iprodione, oligosaccharins, calcium cyanamide, allyl isothiocyanate, fluthiazolin;

[0021] Microorganisms: Aspergullus niger, Paecilomyces lilacinus (Thom.) Samson, Verticillium chlamydosporium, Rhodovulum sulfidophilum HNI-1, Pasteuria pennetrans, Bacillus cereus, Bacillus firmus, Bacillus nematocida, Pseudomonas fluorescens, Trichoderma harzianum, Trichoderma viride, HAN055.

[0022] Preferably, component A in the composition is selected from the above-mentioned piperic acid derivative compounds; component B is selected from thiazolyl, fluopyram, chloropicrin, dazomet, amino oligosaccharide or Paecilomyces lilacinus;

[0023] The weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0024] Further preferably, in the composition, component A is selected from the above-mentioned piperic acid derivative compounds; component B is selected from thiazolyl, fluopyram, chloropicrin, dazomet, amino oligosaccharides, and Paecilomyces lilacinus;

[0025] The weight ratio of the two active components A and B is 1:10 to 10:1.

[0026] The application of the composition is used for preparing nematicides for preventing and controlling plant parasitic nematodes on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops and various plant-related diseases.

[0027] Indoor biological activity assays show that the binary insecticide composition of the present invention has a synergistic effect. For example, compound I-72 and fluopyram show a synergistic effect against southern root-knot nematodes in the range of 10:1 to 1:10.

[0028] The present invention relates to a method for controlling damage to plants and their parts by plant parasitic nematodes (endoparasitic, semi-endoparasitic and ectoparasitic nematodes), in particular plant parasitic nematodes, such as root knot nematodes, Meloidogyne hapla, Meloidogyne incognita, Meloidogyne javanica, Meloidogyne arenaria and other Meloidogyne sp.; cyst-forming nematodes, Globodera rostochiensis and other Globodera species; cereal cyst nematodes (Heterodera avenae), soybean cyst nematodes (Heterodera glycines), beet cyst nematodes (Heterodera schachtii), red clover cyst nematodes (Heterodera schachtii), and so on. trifolii, and other Heterodera spp.; Seed gall nematodes, Anguinas spp.; Stem and foliar nematodes, Aphelenchoides spp.; Sting nematodes, Eelonolaimus longicaudatus, and other Belonolaimus spp.; Pine nematodes, Bursaphelenchus xylophilus, and other Bursaphelenchus spp.; Ringnematodes, Criconema spp., Criconemella spp., Criconemoides spp. spp.), Mesocriconema spp.; Stem and bulb nematodes, Ditylenchus destructor, Ditylenchus dipsaci, and other Ditylenchus spp.); Awl nematodes, Dolichodorus spp.; Spiral nematodes, Heliocotylenchus multicinctus and other Helicotylenchus sp.; Sheath and sheathoid nematodes, Hemicycliophora sp. and Hemicriconemoides spp.; Hirshmanniella spp.; Lance nematodes, Hoploaimus spp.; false rootknot nematodes, Nacobbus spp.; Needle nematodes, Longidorus sp. elongatus and other Longidorus spp.; Pin nematodes, Pratylenchus spp.; Lesion nematodes, Pratylenchus neglectus, Pratylenchus penetrans, Pratylenchus curvitatus, Pratylenchus goodeyi and other Pratylenchus spp.; Burrowing nematodes, Radopholus similis and other Radopholus spp.; Reniform nematodes, Rotylenchus robustus, Rotylenchus reniformis and other Rotylenchus spp.; Scutellonema spp.; Stubby rootnematodes, Trichodorus primitivus and other Trichodorus sp.), Paratrichodorus spp.; Stuntnematodes, Tylenchorhynchus claytoni, Tylenchorhynchus dubius, and other Tylenchorhynchus sp.; Citrus nematodes, Tylenchulus spp.; Daggernematodes, Xiphinema spp.; and other plant-parasitic nematodes, such as Subanguina spp., Hypsoperine spp., Macroposthonia spp., Melinius spp., Punctodera spp.), and Quinisulcius spp.

[0029] Preferably, root knot nematodes, Meloidogyne hapla, Meloidogyne incognita, Meloidogyne javanica, Meloidogyne arenaria and other Meloidogyne spp.;

[0030] Preferably, cyst-forming nematodes, Globodera spp., and other Globodera spp.;

[0031] Preferably, cereal cyst nematode (Heterodera avenae), soybean cyst nematode (Heteroderaglycines), beet cyst nematode (Heterodera schachtii), red clover Heterodera trifolii, and other Heterodera spp.;

[0032] The nematicide composition of the present invention is suitable for controlling pests occurring on agricultural, horticultural, forest and ornamental plants or on tissues and organs of these plants, such as fruits, flowers, leaves, stems, tubers or roots, and other harmful organisms parasitic in organisms.

[0033] The composition of the present invention is applicable to but not limited to the following crops or plants:

[0034] Cereal crops, such as wheat, barley, rye, oats, rice, corn, sorghum, millet, etc.; tuber crops: including sweet potatoes, yams, potatoes, etc.; legume crops: including soybeans, broad beans, peas, mung beans, etc.; fiber crops: including cotton, jute, kenaf, hemp, flax, sisal, straw, etc.; oil crops: including oilseeds, iris, mustard, peanuts, sesame, hemp, sunflower, etc.; sugar crops: including sugarcane, sugar beets, etc.; drinkable Feed crops: including tea, coffee, cocoa, etc.; dye crops: including indigo, saffron, etc.; spice crops: including lemongrass, mint, rose; feed crops: including alfalfa, sweet clover, astragalus, vetch, clover, etc.; feed crops include ryegrass, oat grass, Sudan grass, etc.; green manure crops, such as vetch, astragalus, yellow alfalfa, sweet clover, tamarind, sesbania, amorpha, etc.; fruits, such as apples, pears, plums, peaches, almonds , cherry, citrus, mango, litchi, banana, grape; or berry, such as strawberry, raspberry or blackberry mustard, olive, coconut, castor, etc.; vegetables, such as cucumber, loofah, watermelon, melon, pumpkin, hanging melon, spinach, celery, cabbage, cabbage, cauliflower, gourd, pepper, eggplant, tomato, onion, ginger, garlic, leek, lettuce, kidney bean, cowpea, radish, carrot, yam, etc.; the composition of the present invention can also be used for any ornamental plants Plants, including flowers, shrubs, broadleaf trees and evergreens, for example, violets, begonias, dahlias, gerberas, hydrangeas, verbenas, roses, galanthus, poinsettias, aster, cornflowers, coreopsis, delphiniums, monopetalums, phlox, rhodiola, sedums, petunias, violets, impatiens, geraniums, chrysanthemums, ranunculus, fuchsias, sage, balsam pear, rosemary, sage, St. John's wort, mint.

[0035] Compared with the prior art, the composition of the present invention has the following advantages: the composition has obvious synergistic effect, which improves the control effect on pests; since the mechanism of action of compound I-72 is novel and there is no cross-resistance with existing nematicides, it can be used to control resistant pests. The application of the composition not only expands the insecticidal spectrum of compound I-72, but also delays the generation and development of pest resistance. DETAILED DESCRIPTION

[0036] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products. The active components in the formula examples are metered and added after 100%, and each component is measured by weight percentage.

[0037] Indoor Activity Assay Example

[0038] Example 1: In vitro combined toxicity test of I-72 and fluopyram against southern root-knot nematode

[0039] Test target: Second-instar larvae of southern root-knot nematode [Meloidogyne incognita], a susceptible strain raised in a greenhouse.

[0040] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0041] Test method: The prepared drug solution was added to the 24-well plate in the order of the experimental design dose from low to high. 50 nematodes were placed in each treated well. Five concentrations and three replicates were set for each drug. After treatment, the 24-well plate was placed in a 25-degree constant temperature incubator, and a blank control was set. After 48 hours, the number of dead and live worms was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to obtain the toxicity regression equation, LC for each single test agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 1.

[0042] Table 1 Results of in vitro combined toxicity test of I-72 and fluopyram against southern root-knot nematodes

[0043]

[0044] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the present invention. It can be seen from the above technical scheme that the nematicidal composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing the amount of pesticides used, reducing the amount of pesticide residues on crops, reducing environmental pollution, being safe for humans and animals, having good environmental compatibility, and pests are not easy to develop drug resistance. The composition of the present invention can be applied to the prevention and treatment of nematode-related diseases of various crops and can be widely used in the field of agriculture.

Claims

1. A composition containing a nematicide, characterized in that: The composition comprises two active components A and B, wherein component A is selected from the compounds of the following structure, and component B is fluopyram; the weight ratio of the two active components A and B is 1:20 to 20:1; wherein the compound structural formula is as follows: 。 2. The synergistic composite composition containing two active ingredients according to claim 1, characterized in that: The weight ratio of the two active components A and B is 1:10 to 10:

1.

3. The use of the composition according to claim 1, characterized in that: The composition is used for preparing nematicides for preventing and controlling plant parasitic nematodes on fruit trees, vegetables, ornamental plants, tea, cotton and cereal crops.

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