A method for inducing virus-free original seed potatoes in sweet potato substrate culture

Abstract

The invention discloses a method for inducing virus-free sweet potato matrix culture, which relates to the technical field of plant propagation. The method comprises the following steps: S1, selecting virus-free test tube seedlings of 8-10 cm, training the seedlings in a plant growth room until the growth state is stable to obtain virus-free seedlings, and transferring the virus-free seedlings to a matrix culture device; S2, using zeatin ribosyl monophosphate (ZR) as a solute and 1 / 2 Hoagland nutrient solution as a solvent to prepare a nutrient solution with a ZR concentration of 0.8-16 mg / L, and adding the prepared nutrient solution to the matrix culture device; S3, adding 1 / 2 Hoagland nutrient solution every 40 days after transplanting the seedlings, and cultivating the culture room temperature at 24±2°C, a photoperiod of 14 / 10 h, and a light intensity of 80-100 µmol•m‑2•s‑1. The present invention provides a matrix cultivation method for virus-free seed potatoes, which cultivates virus-free seed potatoes and supplies them to growers instead of the traditional method of supplying virus-free potato seedlings. The growers can use the virus-free seed potatoes to cultivate virus-free seedlings in the production base according to their needs, so as to better meet the market demand for sweet potato production.

Description

Technical Field

[0001] The invention relates to the technical field of plant propagation, in particular to a method for inducing virus-free original seed potatoes for sweet potato substrate cultivation. Background Art

[0002] Sweet potato virus diseases are widespread in sweet potato-producing areas worldwide, causing annual yield losses of 26% to 63% and an average annual economic loss of 4 billion yuan. To date, the most effective method for preventing and controlling sweet potato virus diseases and restoring the quality of the variety is for research institutes or large enterprises with sufficient research equipment to decontaminate the shoot apical meristem and produce virus-free seedlings. These seedlings are then propagated into seed potatoes, which are then propagated into seedlings, which are then propagated into production seedlings. Agricultural cooperatives and large-scale growers then purchase the virus-free production seedlings for propagation and sale. However, current methods for rapid propagation of virus-free sweet potato seedlings are plagued by high propagation costs, significant transportation losses, and complex processes. With the increasing incidence of virus diseases and evolving transmission methods, traditional methods for producing and supplying virus-free seed potato seedlings are no longer sufficient to meet production needs. If the number of sweet potato tubers per plant can be increased and a large number of virus-free seed potatoes can be obtained, virus-free seedlings can be cultivated directly at the production base and used for production, the production of virus-free seedlings can increase exponentially, effectively solving the problem of virus-free sweet potato seed potatoes. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for inducing the culture of virus-free original seed potatoes of sweet potato substrate.

[0004] The object of the present invention is achieved through the following technical solutions:

[0005] A method for inducing the culture of virus-free original seed potatoes of sweet potato substrate comprises the following steps:

[0006] S1. Select 8-10 cm virus-free test tube seedlings, train them in a plant growth chamber until the growth state is stable, and then transfer the virus-free seedlings to a matrix culture container;

[0007] S2. Prepare a nutrient solution with a ZR concentration of 0.8-16 mg / L using zeatin ribosyl monophosphate (ZR) as the solute and 1 / 2 Hoagland nutrient solution as the solvent. Add the prepared nutrient solution to the substrate culture vessel.

[0008] S3. Add 1 / 2 Hoagland nutrient solution every 40 days after transplanting. During cultivation, the room temperature is 24±2℃, the photoperiod is 14 / 10 h, and the light intensity is 80~100 µmol•m-2•s-1.

[0009] The matrix culture device adopts a hydroponic box for cultivation, wherein a flower pot is placed in the hydroponic box, and the nutrient solution is added into the hydroponic box; the virus-free seedlings are moved into the flower pot in the hydroponic box, and the flower pot is filled with a solid matrix, wherein the solid matrix of the present invention adopts perlite.

[0010] In order to prevent excessive evaporation of water and algae growth, the opening of the hydroponic box frame is covered with black plastic film.

[0011] The beneficial effects of the present invention are:

[0012] 1) The present invention provides a matrix cultivation method for virus-free seed potatoes, which cultivates virus-free seed potatoes and supplies them to growers instead of the traditional method of supplying virus-free potato seedlings. Growers can use virus-free seed potatoes to cultivate virus-free seedlings at production bases according to their needs, so as to better meet the market demand for sweet potato production.

[0013] 2) According to the cultivation method provided by the present invention, the stem apical meristem is selected to obtain virus-free seedlings, and the production method of virus-free seed potatoes is further improved. The number of tubers per test tube seedling is regulated by exogenous ZR, and virus-free seed potatoes are efficiently obtained to supply the market for seed potato seedling production.

[0014] 3) The present invention adopts a soilless culture method of substrate culture to cultivate virus-free seed potatoes, which overcomes the problem that sweet potato planting is afraid of waterlogging and can ensure that virus-free seedlings can produce potatoes even under high humidity. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a statistical chart of stem diameter growth;

[0016] Figure 2 This is a statistical chart of root diameter growth;

[0017] Figure 3 This is a statistical chart of the number of tubers per plant;

[0018] Figure 4 This is a statistical chart of single-plant potato weight;

[0019] Figure 5 The figures are actual pictures of potatoes obtained from the examples and comparative examples. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work shall fall within the scope of protection of the present invention.

[0021] The chemicals required for the 1 / 2 Hoagland nutrient solution used in this invention were purchased from Broad Vanke Testing Equipment Sales Co., Ltd. and prepared according to the hydroponic solution formula instructions. The sweet potato used in the experiment was 'Longshu No. 9' (bred by the Longyan Agricultural Science Center in Fujian Province and preserved and provided by the Potato Research and Development Center of Sichuan Agricultural University). The experiment was conducted in the plant growth room of the third teaching building of Sichuan Agricultural University from March to July 2019.

[0022] Matrix culture device used in the embodiment of the present invention is made up of the non-porous plastic frame of length 30 cm, width 20 cm, height 13 cm and 2 plastic flower pots of 12 cm in diameter that are placed in, and perlite is housed in the plastic flower pot, and the interior filling liquid of matrix culture device is 1 / 4 of the non-porous plastic frame height.In order to prevent the excessive evaporation and algae growth of moisture, the non-porous plastic frame mouth is covered with black plastic film, and the hole is inserted in the perlite above the liquid level in the basin during the virus-free seedling planting.Matrix culture device is placed on the three-dimensional culture rack covered with an insect-proof net.

[0023] Example 1 (ZR0.8):

[0024] Take 2L of 1 / 2 Hoagland nutrient solution and 1.6mg ZR to prepare a nutrient solution with a ZR concentration of 0.8mg / L (1 / 2 Hoagland nutrient solution is used as the solvent) for later use.

[0025] 8 cm virus-free test tube seedlings were selected and trained in a plant growth room until the growth state was stable to obtain virus-free seedlings, and the virus-free seedlings were transferred to a matrix culture container;

[0026] Add the prepared nutrient solution to the non-porous plastic frame until the liquid reaches 1 / 4 of the height of the non-porous plastic frame;

[0027] Every 40 days, 1 / 2 Hoagland's nutrient solution was added to the non-porous plastic basket to the initial liquid height. During the cultivation process, the room temperature was 22℃, the photoperiod was 14 / 10 h, and the light intensity was 80 µmol•m-2•s-1.

[0028] Example 2 (ZR8):

[0029] Take 2L of 1 / 2 Hoagland nutrient solution and 16mg ZR to prepare a nutrient solution with a ZR concentration of 8mg / L (1 / 2 Hoagland nutrient solution is used as the solvent) for later use.

[0030] 9 cm virus-free test tube seedlings were selected and trained in a plant growth room until the growth state was stable to obtain virus-free seedlings, and the virus-free seedlings were transferred to a matrix culture container;

[0031] Add the prepared nutrient solution to the non-porous plastic frame until the liquid reaches 1 / 4 of the height of the non-porous plastic frame;

[0032] Every 40 days, 1 / 2 Hoagland's nutrient solution was added to the non-porous plastic basket to the initial liquid height. During the cultivation process, the room temperature was 24℃, the photoperiod was 14 / 10 h, and the light intensity was 90 µmol•m-2•s-1.

[0033] Example 3 (ZR16):

[0034] Take 2L of 1 / 2 Hoagland nutrient solution and 32mg ZR to prepare a nutrient solution with a ZR concentration of 16mg / L (1 / 2 Hoagland nutrient solution is used as the solvent) for later use.

[0035] 10 cm virus-free test tube seedlings were selected and trained in a plant growth room until the growth state was stable to obtain virus-free seedlings, and the virus-free seedlings were transferred to a matrix culture container;

[0036] Add the prepared business solution to the non-porous plastic frame until the liquid is 1 / 4 of the height of the non-porous plastic frame;

[0037] Every 40 days, 1 / 2 Hoagland nutrient solution was added to the non-porous plastic basket to the initial liquid height. During the cultivation process, the room temperature was 22℃, the photoperiod was 14 / 10 h, and the light intensity was 100 µmol•m-2•s-1.

[0038] Comparative Example (CK):

[0039] Different from Example 1, when preparing the nutrient solution, ZR was not added, and 1 / 2 Hoagland nutrient solution was directly used as the nutrient solution.

[0040] Index determination:

[0041] The virus-free seedlings cultivated in Examples 1 to 3 and the comparative example were measured. The stem diameter and root diameter were measured on days 0, 20, 40, 80, and 120 after cultivation. The number of tubers per plant and the weight of tubers per plant were counted on day 130 after cultivation. The growth rate was calculated as follows: stem diameter growth 20 days after treatment = stem diameter 20 days after treatment - stem diameter 0 days after treatment. The stem and root diameter growth rates at other times were calculated in the same way.

[0042] like Figure 1 As shown, 20 to 80 days after treatment, the stem diameter growth of the virus-free seedlings in Examples 2 and 3 was larger; 120 days after transplanting, the stem diameter growth of Example 2 was significantly higher than that of the control example; overall, ZR treatment can significantly thicken the stems of the virus-free seedlings.

[0043] like Figure 2 As shown, the root diameter growth of the virus-free sweet potato seedlings in the examples is greater than that in the comparative example, and the example 1 is particularly significant.

[0044] Depend on Figure 3-5It can be seen that the weight of single tuber in the examples is greater than that in the control example, and the weight of the tuber in the first example is particularly significant. Therefore, the experimental conditions in the examples may be given priority for cultivation during cultivation.

[0045] The foregoing description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concept described herein through the above teachings or techniques or knowledge in the relevant field. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention are intended to be protected by the appended claims.

Claims

1. A method for inducing the culture of virus-free original seed potatoes in a sweet potato matrix, characterized in that: The following steps are involved: S1. Select 8-10 cm virus-free test tube seedlings, train them in a plant growth room until the growth state is stable, and then transfer the virus-free seedlings to a matrix culture container; S2. Prepare a nutrient solution with a zeatin riboside concentration of 0.8-16 mg / L using zeatin riboside as the solute and 1 / 2 Hoagland nutrient solution as the solvent, and add the prepared nutrient solution to the substrate culture vessel; S3. After transplanting, add 1 / 2 Hoagland nutrient solution every 40 days. Cultivate at room temperature of 24±2℃, photoperiod of 14 / 10 h, and light intensity of 80~100 μmol•m -2 •s -1 .

2. The method for culturing virus-free original seed potatoes in a matrix according to claim 1, wherein: The matrix culture device adopts a hydroponic box for cultivation, wherein a flower pot is placed in the hydroponic box, and the nutrient solution is added into the hydroponic box; the virus-free seedlings are moved into the flower pot in the hydroponic box, and the flower pot is filled with a solid matrix.

3. The method for culturing virus-free original seed potatoes in a matrix according to claim 2, wherein: The opening of the hydroponic box frame is covered with a black plastic film.

4. The method for culturing virus-free original seed potatoes in a matrix according to claim 2, wherein: The solid matrix is ​​perlite.

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