An anti-aging composition and its application, and an anti-aging capsule and its application.

Abstract

This invention relates to the field of anti-aging compositions, and more particularly to an anti-aging composition and its application, and an anti-aging capsule and its application. The invention provides an anti-aging composition comprising the following components in parts by weight: 20-2000 parts of calcium α-ketoglutarate, 20-100 parts of quercetin, 20-2000 parts of trans-resveratrol, 20-100 parts of calendula extract, and 0.02-1.8 parts of spermidine. This composition targets 12 major causes of aging, with multi-target synergistic effects, and can safely and effectively reverse aging, enhance physiological function, reduce the risk of chronic diseases, and improve quality of life. The composition is safe, using carefully selected natural food ingredients, ensuring safety and reliability, and has no side effects with long-term use. The oral dosage form is convenient to take, easy to adhere to, and allows for expanded applications.

Description

Technical Field

[0001] This invention relates to the field of anti-aging composition technology, and more particularly to an anti-aging composition and its application, and an anti-aging capsule and its application. Background Technology

[0002] Aging is the strongest risk factor for most chronic diseases, physical and cognitive impairments, and death. Despite this, our approaches to understanding and treating age-related diseases often overlook the biological basis of the aging process. The science of aging suggests that directly targeting aging itself may simultaneously slow the progression of multiple age-related diseases. With the global aging population continuing to grow, the prospect of treating aging to extend healthy lifespan is becoming increasingly promising, and the health problems of the elderly are receiving increasing attention as the aging population intensifies.

[0003] However, aging is a complex physiological process involving multiple mechanisms. Currently, all 12 molecular mechanisms of aging (PMID: 36599349) have been identified: altered intercellular communication, cellular senescence, chronic inflammation, dystrophic nutrient perception, autophagy disorders, gut microbiota dysbiosis, epigenetic changes, genomic instability, loss of protein homeostasis, mitochondrial dysfunction, stem cell depletion, and telomere loss. These represent common aging characteristics across different organisms and species. Current anti-aging products on the market often target only one aging mechanism, lacking comprehensive anti-aging combinations. For example, low-calorie diets and young blood transfusions can partially reverse aging, but low-calorie diets are difficult to quantify and may cause digestive system diseases, including anorexia, or psychological problems. Furthermore, they are difficult to maintain due to their inhumane nature. Young blood transfusions present significant ethical challenges and can lead to inherent transfusion complications such as hemolysis or disease transmission. Therefore, the anti-aging field urgently needs new comprehensive approaches to reverse aging. Summary of the Invention

[0004] The purpose of this invention is to provide an anti-aging composition that targets 12 major causes of aging, with multi-target synergistic effects, and can safely and effectively reverse aging, enhance physiological functions, reduce the risk of chronic diseases, and improve quality of life.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0006] The present invention provides an anti-aging composition comprising the following components in parts by weight: 20-2000 parts of calcium α-ketoglutarate, 20-100 parts of quercetin, 20-2000 parts of trans-resveratrol, 20-100 parts of calendula extract, and 0.02-1.8 parts of spermidine.

[0007] As a preferred embodiment, the composition comprises the following components in parts by weight: 20-2000 parts of calcium α-ketoglutarate, 20-100 parts of quercetin, 20-2000 parts of trans-resveratrol, 20-100 parts of calendula extract, 0.02-1.8 parts of spermidine, 20-2000 parts of glycine, 20-1200 parts of berberine, and 20-500 parts of urolithin A.

[0008] Preferably, the composition includes the following components in parts by weight: 20-2000 parts of calcium α-ketoglutarate, 20-100 parts of quercetin, 20-2000 parts of trans-resveratrol, 20-100 parts of calendula extract, 0.02-1.8 parts of spermidine, 20-2000 parts of glycine, 20-1200 parts of berberine, 20-500 parts of urolithin A, 0.1-1 part of trace lithium, 20-5400 parts of malic acid, 20-608 parts of magnesium, 1-200 parts of hyaluronic acid, 1-300 parts of L-theanine, 1-200 parts of vitamin C, 1-1800 parts of vitamin A, 1-50 parts of vitamin D, and 1-200 parts of 2% curcumin.

[0009] The present invention also provides the use of the described composition in the preparation of anti-aging agents.

[0010] Preferably, the dosage form of the anti-aging preparation is a capsule, tablet, powder, or injection.

[0011] The present invention also provides an anti-aging capsule, comprising a composition and excipients;

[0012] The composition is the composition described above;

[0013] The mass ratio of the composition to the excipients is 5 to 9:1.

[0014] Preferably, the excipients include one or more of rice bran, hydroxypropyl methylcellulose, vegetable magnesium stearate, silicon dioxide, purple carrot, titanium dioxide, propylene glycol, polyvinyl acetate, benzoate, sodium lauryl sulfate, and polyoxyethylene ether 80.

[0015] The present invention also provides the application of the aforementioned capsule in anti-aging.

[0016] The beneficial effects of this invention are:

[0017] The anti-aging compounds of this invention achieve the effects of delaying and reversing aging through the following pathways: Protecting the genome: maintaining genome stability and reducing DNA damage and mutations; extending telomeres: protecting telomere length and delaying cellular aging; regulating epigenetics: optimizing gene expression and maintaining cell function; maintaining protein homeostasis: promoting a balance between protein synthesis and degradation and preventing protein aggregation; enhancing autophagy: clearing damaged cellular components and maintaining cell health; optimizing nutrient sensing: regulating metabolic balance and delaying age-related diseases; enhancing mitochondrial function: improving energy production efficiency and reducing oxidative stress; inhibiting cellular senescence: prolonging cell lifespan and maintaining tissue function; activating stem cells: promoting tissue regeneration and repairing aging damage; improving intercellular communication: coordinating cellular function and maintaining tissue integrity; inhibiting inflammation: reducing the damage of chronic inflammation to cells and tissues; regulating gut microbiota: maintaining gut microbiota balance and promoting overall health.

[0018] The composition of this invention targets 12 major causes of aging, with multi-target synergistic effects. It can reduce aging biomarkers (epigenetic age) in mammals, enhance their motor function, improve metabolic disorders, reverse the increase in liver epigenetic age caused by a high-fat diet, enhance memory, reduce skin wrinkles, and increase skin collagen content, thereby safely and effectively reversing aging, enhancing physiological function, reducing the risk of chronic diseases, and improving quality of life. The composition is safe, made from carefully selected natural food ingredients, and is safe and reliable with no side effects from long-term use. The oral dosage form is convenient to take, easy to adhere to, and allows for expanded application methods. Attached Figure Description

[0019] Figure 1 The percentage of individuals in the normal diet group whose epigenetic age decreased;

[0020] Figure 2 The percentage of individuals in the high-fat diet group who showed a decrease in epigenetic age.

[0021] Figure 3 The results of the athletic ability test are shown in Figure A, where the speed multiplier changes are shown, and Figure B shows the endurance time multiplier changes.

[0022] Figure 4 To improve glucose tolerance after a high-fat diet;

[0023] Figure 5 The results of the Morris maze test;

[0024] Figure 6 The results show the skin wrinkles and collagen content. Detailed Implementation

[0025] The present invention provides an anti-aging composition comprising the following components in parts by weight: 20 to 2000 parts of calcium α-ketoglutarate, preferably 520 to 1500 parts, and more preferably 1010 parts;

[0026] Quercetin 20-100 parts, preferably 50-70 parts, more preferably 60 parts;

[0027] Trans-resveratrol: 20-2000 parts, preferably 520-1500 parts, and more preferably 1010 parts;

[0028] Calendula extract: 20-100 parts, preferably 50-70 parts, and more preferably 60 parts;

[0029] The amount of spermidine is 0.02 to 1.8 parts, preferably 0.52 to 1.3 parts, and more preferably 0.91 parts.

[0030] In this invention, calcium α-ketoglutarate can provide key metabolites for mitochondria to produce cellular energy, helping mitochondria generate cellular energy and improve motility; quercetin can enhance the immune system's immunity and clear "zombie" aging cells and dysfunctional proteins; trans-resveratrol has the function of activating sirtuins' DNA genome repair function, which can maintain the stability of the genome and epigenome; calendula extract can clear "zombie" aging cells and enhance the body's immunity; spermidine has the effect of enhancing mitochondrial phagocytosis and mitochondrial function.

[0031] In this invention, the component comprises the following parts by mass: 20 to 2000 parts of calcium α-ketoglutarate, preferably 520 to 1500 parts, and more preferably 1010 parts;

[0032] Quercetin 20-100 parts, preferably 50-70 parts, more preferably 60 parts;

[0033] Trans-resveratrol: 20-2000 parts, preferably 520-1500 parts, more preferably 1010 parts;

[0034] Calendula extract: 20-100 parts, preferably 50-70 parts, and more preferably 60 parts;

[0035] The amount of spermidine is 0.02 to 1.8 parts, preferably 0.52 to 1.3 parts, and more preferably 0.91 parts;

[0036] The glycine content is 20 to 2000 parts, preferably 520 to 1500 parts, and more preferably 1010 parts;

[0037] Berberine 20-1200 parts, preferably 420-800 parts, more preferably 610 parts;

[0038] The amount of urolithiasis A is 20 to 500 parts, preferably 220 to 300 parts, and even more preferably 260 parts.

[0039] In this invention, glycine has the effect of prolonging the lifespan of mammals, with the same effect as "methionine restriction diet" (i.e., prolonging the lifespan of mammals), and enhancing the ability of mitochondrial autophagy; berberine can effectively activate the longevity-promoting AMPK signal and maintain blood sugar and metabolic balance; urolithin A has the effects of enhancing mitochondrial autophagy and enhancing muscle function.

[0040] In this invention, the component comprises the following parts by mass: 20 to 2000 parts of calcium α-ketoglutarate, preferably 520 to 1500 parts, and more preferably 1010 parts;

[0041] Quercetin 20-100 parts, preferably 50-70 parts, more preferably 60 parts;

[0042] Trans-resveratrol: 20-2000 parts, preferably 520-1500 parts, more preferably 1010 parts;

[0043] Calendula extract: 20-100 parts, preferably 50-70 parts, and more preferably 60 parts;

[0044] The amount of spermidine is 0.02 to 1.8 parts, preferably 0.52 to 1.3 parts, and more preferably 0.91 parts;

[0045] The glycine content is 20 to 2000 parts, preferably 520 to 1500 parts, and more preferably 1010 parts;

[0046] Berberine 20-1200 parts, preferably 420-800 parts, more preferably 610 parts;

[0047] Urolithiasis A: 20-500 parts, preferably 220-300 parts, more preferably 260 parts.

[0048] The trace amount of lithium is 0.1 to 1 part, preferably 0.5 to 0.6 parts, and more preferably 0.55 parts;

[0049] The amount of malic acid is 20 to 5400 parts, preferably 2020 to 3400 parts, and more preferably 2710 parts;

[0050] Magnesium: 20-608 parts, preferably 220-408 parts, more preferably 314 parts;

[0051] Hyaluronic acid: 1-200 parts, preferably 51-150 parts, more preferably 100.5 parts;

[0052] L-theanine: 1-300 parts, preferably 101-200 parts, more preferably 150.5 parts;

[0053] Vitamin C 1-200 parts, preferably 51-150 parts, more preferably 100.5 parts;

[0054] Vitamin A 1-1800 parts, preferably 801-1000, more preferably 900.5 parts;

[0055] Vitamin D 1-50 parts, preferably 21-30 parts, more preferably 25.5 parts;

[0056] 1-200 parts of 2% curcumin, preferably 51-150 parts, and even more preferably 100.5 parts.

[0057] In this invention, trace amounts of lithium can extend the lifespan of various species, and supplementing with trace amounts of lithium can promote healthy brain aging; malic acid, a natural substance found in apples, has been shown to extend the lifespan of organisms and can also help humans maintain healthy blood sugar levels; magnesium supports healthy metabolism and targets inflammation at the cellular level, and people who supplement with magnesium show less DNA damage; malic acid and magnesium are often found together as magnesium malate; studies have shown that acetylglucosamine, a component of hyaluronic acid, has been shown to extend the lifespan of animals, and oral intake of hyaluronic acid can reduce the appearance of wrinkles; theanine is one of the substances responsible for the health effects of green tea, and theanine can extend the lifespan of various organisms, increase the production of aging-protective proteins such as FOXO1 and antioxidant enzymes, and help the body manage cross-linking; vitamin C helps maintain a healthy epigenome, especially when used in combination with α-ketoglutarate; vitamins A and D help reduce the epigenetic age of men, especially when used in combination with α-ketoglutarate.

[0058] The present invention also provides the use of the described composition in the preparation of anti-aging agents.

[0059] In this invention, the dosage form of the anti-aging preparation is a capsule, tablet, powder, or injection.

[0060] The present invention also provides an anti-aging capsule, comprising a composition and excipients;

[0061] The composition is the composition described above;

[0062] The mass ratio of the composition to the excipients is 5 to 9:1, preferably 7:1.

[0063] In this invention, the excipients include one or more of rice bran, hydroxypropyl methylcellulose, vegetable magnesium stearate, silicon dioxide, purple carrot, titanium dioxide, propylene glycol, polyvinyl acetate, benzoate, sodium lauryl sulfate, and polyoxyethylene ether 80.

[0064] In this invention, if the excipients used are rice bran, hydroxypropyl methylcellulose, magnesium stearate from vegetables, silicon dioxide, purple carrot, titanium dioxide, propylene glycol, polyvinyl acetate, benzoate, sodium lauryl sulfate, and polyoxyethylene ether 80 in a weight ratio of 2 to 60:2, then the mass ratio of rice bran, hydroxypropyl methylcellulose, magnesium stearate from vegetables, silicon dioxide, purple carrot, titanium dioxide, propylene glycol, polyvinyl acetate, benzoate, sodium lauryl sulfate, and polyoxyethylene ether 80 is 2 to 60:2. 0~92:1~10:0.2~1:1~4:0.2~1:2~15:1~8:1~8:1~10:2.5~7, preferably 22~40:40~72:4~7:0.5~0.7:2~3:0.5~0.7:7~10:3~6:3~6:4~7:3.5~6, further preferably 31:56:5.5:0.6:2.5:0.6:8.5:4.5:4.5:5.5:4.75.

[0065] The present invention also provides the application of the aforementioned capsule in anti-aging.

[0066] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0067] Example 1: An anti-aging capsule

[0068] (1) Weighing the composition: Weigh 1000mg of α-ketoglutarate calcium, 100mg of quercetin, 500mg of trans-resveratrol, 100mg of calendula extract and 900μg of spermidine and mix them to prepare the composition;

[0069] (2) Weigh out the following excipients: 90mg rice bran, 540mg hydroxypropyl methylcellulose, 45mg magnesium stearate for vegetables, 4.5mg silicon dioxide, 18mg purple carrot, 4.5mg titanium dioxide, 18mg propylene glycol, 45mg polyvinyl acetate, 45mg benzoate, 45mg sodium lauryl sulfate and 45mg polyoxyethylene ether 80 are mixed to prepare the excipients;

[0070] (3) Preparation of capsules: The above composition and excipients are mixed in a ratio of 9:1 and the anti-aging capsules are prepared in a fully automated GMP environment; the cap of a single capsule is 11.50 mm long, the wall thickness is 0.090 mm, the outer diameter is 8.45 mm, the capsule body is 20.00 mm long, the wall thickness is 0.090 mm, the outer diameter is 8.10 mm, and the total effective ingredients are 900 mg.

[0071] Example 2: An anti-aging capsule

[0072] (1) Weigh the composition: Weigh 1000mg of α-ketoglutarate calcium, 100mg of quercetin, 500mg of trans-resveratrol, 100mg of calendula extract, 900μg of spermidine, 1000mg of glycine, 600mg of berberine, and 250mg of urolithiasis A and mix them to prepare the composition.

[0073] (2) Weigh the excipients as in Example 1;

[0074] (3) The preparation of the capsules is described in Example 1.

[0075] Example 3: An anti-aging capsule

[0076] (1) Weighing the composition: Weigh 1000mg of α-ketoglutarate calcium, 100mg of quercetin, 500mg of trans-resveratrol, 100mg of calendula extract, 900μg of spermidine, 1000mg of glycine, 600mg of berberine, 250mg of urolithiasis A, 0.5mg of trace lithium, 2700mg of malic acid, 302mg of magnesium, 100mg of hyaluronic acid, 150mg of L-theanine, 100mg of vitamin C, 900mg of vitamin A, 25mg of vitamin D, and 100mg of 2% curcumin and mix them to prepare the composition;

[0077] (2) Weigh the excipients as in Example 1;

[0078] (3) The preparation of the capsule is described in Example 1.

[0079] Comparative Example 1: An anti-aging capsule

[0080] (1) Weighing the composition: Weigh 1000mg glycine, 600mg berberine and 250mg urolithiasis A and mix them to prepare the composition;

[0081] (2) Weigh the excipients as in Example 1;

[0082] (3) Preparation of capsules (Refer to Example 1)

[0083] Experimental Example 1: Changes in Liver Epigenetic Age

[0084] Preparation of experimental mice: Sixty-eight male C57BL / 6J wild-type (WT) mice (4 months old, purchased from JacksonLab) were selected and randomly divided into 17 treatment groups as shown in Table 1.

[0085] Table 1 Feeding methods for the treatment groups

[0086]

[0087] Note: The standard feed was LabDiet 5053, purchased from Labdiet; the high-fat Ain-93 feed was purchased from TestDiet and was formulated by adding hydrogenated coconut oil to provide 60% of the calories; the calorie diet was formed by gradually reducing the food intake by 10% each week to the remaining 30%; β-nicotinamide mononucleotide was purchased from Sigma.

[0088] All treatment groups were administered the drug to mice via gavage at a dose of 300 mg / kg once a day. The mice were fed freely at a dose of 5 g / day per mouse.

[0089] After 10 months of rearing, mouse livers were collected, and genomic DNA was extracted and purified using the DNeasyBlood and Tissue Kit (Qiagen). Then, following a slight modification of the method in literature PMID 28380383, a corresponding RRBS methylation gene chip (Illumia) library was constructed. The methylation site profile was determined using the Illumia RRBS methylation chip, and the following bioinformatics algorithms (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / , http: / / www.bioinformatics.babraham.ac.uk / projects / trim_galore / , http: / / www.bioinformatics.babraham.ac.uk / projects / bismark / ) were used to confirm the methylation site profile. The methylation data was then input into a specific epigenetics calculation website (https: / / dnamage.genetics.ucla.edu / home) to calculate the epigenetic age according to the method in literature PMID38200273. The specific steps are as follows:

[0090] (1) Genomic DNA extraction and purification were performed according to the above kit. Sample processing: The tissue (25 mg mouse liver tissue) was cut into small pieces and placed in a 1.5 ml microcentrifuge tube. 180 μl of Buffer ATL and 20 μl of proteinase K were added. The mixture was vortexed and incubated at 56 °C until complete lysis. During incubation, the mixture was vortexed and mixed. After vortexing for 15 s, 200 μl of Buffer ATL was added. The mixture was vortexed and mixed thoroughly. After incubating the sample at 56 °C overnight, 200 μl of ethanol (96%) was added and vortexed and mixed thoroughly. The mixture was aspirated into a DNeasyMini column in a 2 ml waste collection tube and centrifuged at 8000 rpm for 1 min. The effluent was discarded and the waste collection tube was reused. 500 μl of Buffer ATL was added and centrifuged at 8000 rpm for 1 min. The effluent was discarded and the waste collection tube was reused. 500 μl of Buffer ATL was added and centrifuged at 8000 rpm for 1 min. The effluent was discarded and the waste collection tube was reused. Buffer AW2 and centrifuge at 14000 rpm for 3 min, discard the effluent and waste collection tube, and transfer the purification column; transfer the column to a new 2 ml microcentrifuge tube, and then elute DNA by adding 200 μl Buffer AE to the center of the column membrane; incubate at 25 °C for 1 min, centrifuge at 8000 rpm for 1 min to complete the extraction and purification of mouse liver genomic DNA;

[0091] (2) Establishment of RRBS library: First, genomic DNA is fragmented by restriction endonuclease, that is, after genomic DNA is extracted, it is treated with restriction endonuclease Msp I and then the ends are repaired and aptamers are ligated. That is, after digesting genomic DNA, the CG protrusion at the 5' end is repaired and the A tail is added; Second, the size selection of genomic DNA fragmentation: Once the aptamers are ligated, the DNA is loaded onto an agarose gel and fragments of 40-120 bp and 120-220 bp in length are separated for sulfite conversion;

[0092] (3) Sulfite conversion: Sulfite conversion deamination of unmethylated cytosine converts it into uracil residues, which are ultimately read as thymine in the analysis; methylated cytosine is protected and will not be converted by sulfite, so it is still read as cytosine in the analysis.

[0093] (4) PCR amplification: Before sequencing, the sulfite-converted DNA is amplified by PCR;

[0094] (5) Sequencing: The amplified library was subjected to next-generation sequencing on the Illumina NGS platform;

[0095] (6) Bioinformatics to obtain baseation site profiles: After sequencing is completed, bioinformatics analysis is performed, including sequence alignment and methylation identification;

[0096] (7) Input the obtained methylation profile data into the publicly available epigenetic age calculator (https: / / dnamage.genetics.ucla.edu / home) and calculate the liver epigenetic age according to the method in literature PMID38200273. The calculation results are as follows: Figures 1-2 As shown.

[0097] from Figure 1 As can be seen, Formula 1 and Formula 3 alone can reduce the epigenetic age of elderly people with normal diets. Formula 2 can enhance the effect of Formula 1 in reducing the epigenetic age of elderly people with normal diets. The effect of Formula 2 alone is not obvious. The effect of Formula 1 + Formula 2 is better than Formula 3 alone (the anti-aging capsule described in Example 3). No single component in Formula 1 + Formula 2 has the effect of reducing the epigenetic age of elderly people with normal diets.

[0098] from Figure 2 As can be seen, Formula 1 + Formula 2 can reverse the epigenetic age increase caused by a high-fat diet; a 30% calorie restriction can reduce the body's epigenetic age, and Formula 1 + Formula 2 is more effective than Formula 3 in reducing epigenetic age than 30% calorie restriction; NMN cannot reduce epigenetic age. Therefore, the anti-aging capsules described in Examples 1-3 can reduce the epigenetic age increase caused by a high-fat diet.

[0099] Experiment Example 2: Measurement of Motor Ability

[0100] Preparation of experimental mice: Thirty-two male C57BL / 6J wild-type (WT) mice (4 months old, purchased from JacksonLab) were randomly divided into eight treatment groups: high-fat diet group (high-fat Ain-93 diet, purchased from TestDiet, formed by adding hydrogenated coconut oil to provide 60% of the calories), 30% calorie diet (formed by gradually reducing food by 10% each week to the remaining 30%), high-fat diet group + formula 1 (anti-aging capsules of Example 1), high-fat diet group formula 2 (anti-aging capsules of Comparative Example 2), high-fat diet group + formula 1 + formula 2 (anti-aging capsules of Example 2), high-fat diet group + formula 3 (anti-aging capsules as described in Example 3), NMN (regular diet + β-nicotinamide mononucleotide, purchased from Sigma), and normal diet group (regular diet). All treatment groups were administered the drugs to the mice by gavage at a dose of 300 mg / kg once a day.

[0101] Motor ability was assessed using an electric treadmill (model 1050EXER 3 / 6; Columbus Instruments). Three days prior to the test, mice were gradually acclimatized by walking on the treadmill for 10 minutes daily at a speed of 10 m / min. At the start of the test, the mice began walking at 10 m / min, increasing the speed by 2 m / min every 2 minutes until exhaustion was achieved. The time to exhaustion and maximum speed were recorded. Testing began when the mice were 4 months old and was conducted every 2 weeks for 6 months. Test results are as follows: Figure 3 As shown in the figure, Figure A represents the change in speed ratio, and Figure B represents the change in tolerance time ratio.

[0102] from Figure 3 As can be seen, Formula 1 and Formula 3 alone can improve the body's athletic ability, namely, increasing maximum speed and endurance time. Formula 2 can enhance the effect of Formula 1 in improving athletic ability. The combined effect of Formula 1 and Formula 2 is better than Formula 3. A 30% calorie restriction can improve athletic ability, while NMN cannot enhance athletic ability. Therefore, the anti-aging capsules described in Examples 1-3 can effectively improve the body's athletic ability.

[0103] Experiment Example 3: Glucose Tolerance Test

[0104] Preparation of experimental mice: Twenty male C57BL / 6J wild-type (WT) mice (4 months old, purchased from JacksonLab) were randomly divided into 5 treatment groups: a normal diet group (fed conventional feed without any added drugs) as the control group, and a control group fed a completely high-fat diet (high-fat Ain-93 diet, purchased from TestDiet, which is formed by adding hydrogenated coconut oil to provide 60% of the calories) with the following drugs added: Formula 1 (anti-aging capsules described in Example 1), Formula 2 (anti-aging capsules described in Comparative Example 1), Formula 1 + Formula 2 (anti-aging capsules of Example 2), and Formula 3 (anti-aging capsules of Example 3). All treatment groups were administered the drugs to the mice by gavage at a dose of 300 mg / kg once a day.

[0105] After 10 months of feeding, the mice were fasted for 12 hours and then injected intraperitoneally with glucose (Sigma-Aldrich) at a dose of 2 g / kg per mouse. Blood samples were collected at 0, 15, 30, 60, and 120 minutes after injection. Serum insulin concentration was measured using the UltraSensitive MouseInsulin ELISA kit (Crystal Chem, 90080). The results are shown below. Figure 4 As shown.

[0106] from Figure 4As can be seen, Formula 1 + Formula 2 can improve glucose tolerance after a high-fat diet, while Formula 1 alone, Formula 2 alone, Formula 3 alone, and a normal diet cannot improve glucose tolerance after a high-fat diet. Therefore, the anti-aging capsules described in Examples 1-3 can improve abnormal glucose tolerance after a high-fat diet and treat metabolic disorders in mice on a high-fat diet.

[0107] Experiment Example 4: Morris Water Maze Experiment

[0108] Preparation of experimental mice: Twenty male C57BL / 6J wild-type (WT) mice (4 months old, purchased from JacksonLab) were randomly divided into 5 treatment groups: normal diet group (fed conventional feed without any drugs), and the following drugs were added to the conventional diet: Formula 1 (anti-aging capsules described in Example 1), Formula 2 (anti-aging capsules described in Comparative Example 1), Formula 1 + Formula 2 (anti-aging capsules of Example 2), and Formula 3 (anti-aging capsules of Example 3). All treatment groups were administered the drugs to the mice by gavage at a dose of 300 mg / kg once a day.

[0109] The Morris water maze system (purchased from ConductScience) has a diameter of 90 cm and a height of 45 cm. The inner walls are painted black, and the maze is filled with tap water at a temperature maintained at 23°C. The escape platform has a diameter of 6 cm and a height of 24 cm, located in the center of the fourth quadrant of the maze, 1 cm below the water surface. A camera is installed directly above the maze, connected to the equipment analysis system, to collect images and data. The maze is divided into four quadrants: Q1, Q2, Q3, and Q4, with Q3 being the target quadrant, including the escape platform Q5. The experiment consisted of two phases: a training phase and a testing phase. During the training phase, mice were randomly placed in the water in four different quadrants. The software recorded the time it took for the mouse to enter the water and climb onto the platform; this was called the escape latency. Each mouse underwent four training sessions per day (entering the water from different quadrants), with each session lasting 1 minute, for 5 consecutive days. If a mouse failed to find the platform within 1 minute, it was manually guided to find it and rest for 1 minute. During the testing phase, all mice were placed in water from a fixed quadrant (excluding the target quadrant). The software recorded the data for 1 minute and observed their process of finding a safe platform. The escape latency (s) was used as the final observation indicator. The test results are as follows: Figure 5 As shown.

[0110] from Figure 5As can be seen, Formula 1 + Formula 2, Formula 1 alone, and Formula 3 alone can improve the body's memory ability. Formula 2 can enhance the memory-enhancing effect of Formula 1, while Formula 2 alone has no significant effect. Formula 1 + Formula 2 is more effective than Formula 3. Therefore, the anti-aging capsules described in Examples 1-3 can improve the body's memory ability.

[0111] Experimental Example 5

[0112] Preparation of experimental mice: Twenty-four male C57BL / 6J wild-type (WT) mice (4 weeks old, purchased from JacksonLab) were randomly divided into six treatment groups: 4-week-old mice on a normal diet served as a blank control; the experimental groups were: normal diet group (fed conventional feed without any added drugs), and the following drugs were added to the conventional feed: Formula 1 (anti-aging capsules described in Example 1), Formula 2 (anti-aging capsules described in Comparative Example 1), Formula 1 + Formula 2 (anti-aging capsules from Example 2), and Formula 3 (anti-aging capsules from Example 3). All drugs in the above experimental groups were administered to the mice by gavage at a dose of 300 mg / kg once a day for 10 months.

[0113] The dorsal wrinkles of nude mice were examined and photographed after 10 months of normal diet and experimental feeding at 4 weeks of age. The wrinkles were quantified using NIH ImagineJ software. Simultaneously, the full-thickness skin of the corresponding dorsal region of the nude mice was removed and fixed with 4% paraformaldehyde. The fixed skin tissue was gradually dehydrated in ethanol solutions of different concentrations, typically starting with a low concentration (70%) and gradually transferring to a higher concentration (100%). The dehydrated skin tissue was then placed in a suitable organic solvent (pentanol) to remove fat and make the tissue transparent. The degreased skin tissue was then immersed in liquid paraffin or molten wax and solidified at low temperature. The solidified skin tissue was cut using a rotary microtome (Thermo Fisher) to obtain sections (10 μm thick). The sections were placed in a staining cassette and stained with Masson's Trichrome (lab Vector) to specifically stain collagen fibers. The stained sections were washed under running water to remove excess dye, mounted, photographed under a microscope (Leica), and then entered into the NIH ImagineJ software. J software calculates skin collagen content, wrinkle gross observation score, and changes in collagen III absorption fold, as shown below. Figure 6 As shown.

[0114] The standard for the gross wrinkle observation score is as follows (PMID:2169296): 0 points: normal skin with fine texture; 1 point: skin with fine abrasions; 2 points: a few shallow wrinkles; 3 points: many shallow wrinkles; 4 points: rough skin with deep wrinkles; 5 points: many rough wrinkles; 6 points: rough wrinkles with local skin damage.

[0115] from Figure 6 As can be seen, Formula 1 + Formula 2, Formula 1 alone, and Formula 3 alone can reduce skin wrinkles and increase collagen III content. Formula 2 can enhance the effect of Formula 1, while Formula 2 alone has no significant effect. Formula 1 + Formula 2 is more effective than Formula 3. Therefore, the anti-aging capsules described in Examples 1-3 can effectively reduce skin wrinkles and increase skin collagen content.

[0116] Example 4: An anti-aging capsule

[0117] (1) Weigh the composition: Weigh 1200mg of α-ketoglutarate calcium, 80mg of quercetin, 1000mg of trans-resveratrol, 50mg of calendula extract and 1000μg of spermidine and mix them to prepare the composition;

[0118] (2) Weigh out the following excipients: 360mg rice bran, 675mg hydroxypropyl methylcellulose, 90mg magnesium stearate for vegetables, 15mg silicon dioxide, 45mg purple carrot, 15mg titanium dioxide, 45mg propylene glycol, 90mg polyvinyl acetate, 60mg benzoate, 60mg sodium lauryl sulfate and 45mg polyoxyethylene ether 80 are mixed to prepare the excipients;

[0119] (3) Preparation of capsules: The above composition and excipients are mixed in a 7:1 ratio and the anti-aging capsules are prepared in a fully automated GMP environment; the cap of a single capsule is 11.50 mm long, the wall thickness is 0.090 mm, the outer diameter is 8.45 mm, the capsule body is 20.00 mm long, the wall thickness is 0.090 mm, the outer diameter is 8.10 mm, and the total effective ingredients are 900 mg.

[0120] Example 5: An anti-aging capsule

[0121] (1) Weigh the composition: Weigh 1200mg of α-ketoglutarate calcium, 80mg of quercetin, 1000mg of trans-resveratrol, 50mg of calendula extract, 1000μg of spermidine, 1500mg of glycine, 1000mg of berberine and 400mg of urolithiasis A and mix them to prepare the composition.

[0122] (2) Weigh the excipients as described in Example 4;

[0123] (3) The preparation of the capsules is described in Example 4.

[0124] Example 6: An anti-aging capsule

[0125] (1) Weighing the composition: Weigh 1200mg α-ketoglutarate calcium, 80mg quercetin, 1000mg trans-resveratrol, 50mg calendula extract, 1000μg spermidine, 1500mg glycine, 1000mg berberine, 400mg urolithiasis A, 1mg trace lithium, 3000mg malic acid, 600mg magnesium, 150mg hyaluronic acid, 200mg L-theanine, 50mg vitamin C, 700mg vitamin A, 40mg vitamin D, and 150mg 2% curcumin and mix them to prepare the composition;

[0126] (2) Weigh the excipients as described in Example 4;

[0127] (3) The preparation of the capsules is described in Example 4.

[0128] As can be seen from the above embodiments, the present invention provides an anti-aging composition and its application, and an anti-aging capsule and its application. The composition targets 12 major causes of aging, with multi-target synergistic effects, and can safely and effectively reverse aging, enhance physiological functions, reduce the risk of chronic diseases, and improve quality of life. The composition is safe, made from carefully selected natural food ingredients, and is safe and reliable with no side effects from long-term use. The oral dosage form is convenient to take, easy to adhere to, and can be used in various ways.

[0129] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-aging composition, characterized in that, It includes the following components in parts by weight: 1000 mg of calcium α-ketoglutarate, 100 mg of quercetin, 500 mg of trans-resveratrol, 100 mg of calendula extract, 900 μg of spermidine, 1000 mg of glycine, 600 mg of berberine, and 250 mg of urolithiasis A.

2. The use of the composition according to claim 1 in the preparation of anti-aging agents.

3. The application according to claim 2, characterized in that, The dosage form of the anti-aging preparation is capsule, tablet, powder or injection.

4. An anti-aging capsule, characterized in that, Includes compositions and excipients; The composition is the composition according to claim 1; The mass ratio of the composition to the excipients is 5~9:

1.

5. The capsule according to claim 4, characterized in that, The excipients include one or more of rice bran, hydroxypropyl methylcellulose, magnesium stearate from vegetables, silicon dioxide, purple carrot, titanium dioxide, propylene glycol, polyvinyl acetate, benzoate, sodium lauryl sulfate, and polyoxyethylene ether 80.

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