Method of preparing a bordeaux leaf extract, skin care products and uses thereof

Abstract

Embodiments of the present disclosure disclose a method for preparing a bordeaux leaf extract, a skin care product and uses thereof. One specific embodiment of the method comprises: freezing and pulverizing a bordeaux leaf to obtain a bordeaux leaf powder; mixing the bordeaux leaf powder with a vegetable oil to obtain a first mixture; mixing the first mixture with an emulsifier to obtain a second mixture; microwave extracting the second mixture after standing to obtain a first extract; ultrasonic extracting the first extract to obtain a second extract; performing a suction filtration treatment on the second extract to obtain a filtrate as a third extract; and performing a centrifugal treatment on the third extract to obtain a supernatant as the bordeaux leaf extract. The embodiment can prepare a bordeaux leaf extract with better activity.

Description

TECHNICAL FIELD

[0001] Embodiments of the present disclosure relate to the technical field of cosmetics, in particular to a preparation method of boldo leaf extract, a skin care product and use thereof. BACKGROUND

[0002] Peumus boldus Molina is a shrub belonging to Monimiaceae and distributed in Chile. The boldo leaf contains active ingredients such as alkaloids, phenolic compounds and essential oils. In particular, boldine contained in the boldo leaf is a relatively high content alkaloid. Due to its antioxidant, anti-inflammatory, cell protection and other effects, the boldo leaf has applications in liver protection, urinary tract infection treatment, and indigestion treatment.

[0003] However, when the boldo leaf extract is extracted by the above method, the following technical problem I exists:

[0004] Alcohol organic solvents often need to be heated during extraction, and high temperature can cause the active ingredients contained in the boldo leaf to be decomposed or denatured, resulting in low activity or even failure of the boldo leaf extract obtained.

[0005] In addition, skin is the interface between the body and the external environment, and is the first line of defense against adverse external environment. In practice, compared with other organs, skin often shows signs of aging earlier with age and environmental factors, such as skin laxity and wrinkle formation. At present, when repairing and protecting the skin, the skin repair and protection is usually performed from the symptom level (for example, reducing skin redness, shrinking fine pores, and improving skin water-oil balance), which leads to the following technical problem II: there is a lack of effective skin repair and protection from the cause level, especially from the light cause level, resulting in the skin repair and protection effect to be improved. SUMMARY

[0006] The summary of the present disclosure is used to introduce the concepts in a brief form, which will be described in detail in the specific embodiments part. The summary of the present disclosure is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to limit the scope of the claimed technical solutions.

[0007] Some embodiments of the present disclosure propose a preparation method of a bordeaux leaf extract, a skin care product and use to solve one or more of the technical problems mentioned in the background section.

[0008] In a first aspect, some embodiments of the present disclosure provide a preparation method of a bordeaux leaf extract, comprising: freezing and crushing a bordeaux leaf to obtain a bordeaux leaf powder; mixing the bordeaux leaf powder with a vegetable oil to obtain a first mixture; mixing the first mixture with an emulsifier to obtain a second mixture; performing microwave extraction on the second mixture after standing to obtain a first extract; performing ultrasonic extraction on the first extract to obtain a second extract; performing filtration on the second extract to obtain a filtrate as a third extract; and performing centrifugation on the third extract to obtain a supernatant as the bordeaux leaf extract.

[0009] Optionally, the vegetable oil includes one or more of jojoba oil, rice germ oil, rapeseed oil, olive oil, squalane, sunflower oil, and white pool flower oil.

[0010] Optionally, the emulsifier is a polyglycerol-based emulsifier, and the emulsifier includes one or more of polyglyceryl-2 triisostearate, polyglyceryl-10 isostearate, polyglyceryl-2 isostearate, polyglyceryl-2 dipolyhydroxystearate, polyglyceryl-3 diisostearate, polyglyceryl-2 isostearate / dipolyunsaturated linoleate copolymer, polyglyceryl-2 sesquiisocaprylate, and polyglyceryl-2 stearate.

[0011] Optionally, before the microwave extraction on the second mixture after standing to obtain the first extract, the method further includes: standing the second mixture at a standing temperature in a range from 4 degrees Celsius to 35 degrees Celsius for 24 hours to 72 hours to obtain the second mixture after standing.

[0012] Optionally, the microwave extraction on the second mixture after standing to obtain the first extract includes: performing cyclic microwave extraction on the second mixture after standing for a target number of times at a microwave extraction power in a range from 200 watts to 800 watts to obtain the first extract, wherein the target number of times is 2 to 4, a single cycle of microwave extraction has a duration of 5 minutes to 10 minutes, and a time interval between two consecutive cycles of microwave extraction is 2 minutes to 10 minutes.

[0013] Optionally, the ultrasonic extraction on the first extract to obtain the second extract comprises: performing cyclic ultrasonic extraction on the first extract for a target number of times to obtain the second extract, wherein the target number of times is 2-4, and the extraction time of a single cycle of ultrasonic extraction is 15-30 minutes.

[0014] Optionally, the centrifugal treatment on the third extract to obtain the supernatant as the extract of the leaves of the vine Vitis vinifera Linn. comprises: centrifugal treatment on the third extract at a centrifugal temperature of 4-20 degrees Celsius and a centrifugal speed of 3600-5000 rpm for 5-15 minutes, and collecting the supernatant as the extract of the leaves of the vine Vitis vinifera Linn.

[0015] In the second aspect, some embodiments of the present disclosure provide a skin care product comprising the extract of the leaves of the vine Vitis vinifera Linn. as described in the first aspect.

[0016] In the third aspect, some embodiments of the present disclosure provide a use of the extract of the leaves of the vine Vitis vinifera Linn. comprising the use of the extract of the leaves of the vine Vitis vinifera Linn. as described in the first aspect in anti-photoaging and photodamage repair.

[0017] In the fourth aspect, some embodiments of the present disclosure provide a use of the skin care product comprising the use of the skin care product as described in the second aspect in anti-photoaging and photodamage repair.

[0018] The above various embodiments of the present disclosure have the following beneficial effects: through the preparation method of the Bordeaux leaf extract of some embodiments of the present disclosure, the Bordeaux leaf extract with better activity can be prepared. Specifically, the reason why the activity of the obtained Bordeaux leaf extract is poor or even inactivated is that the alcohol organic solvent needs to be heated during the extraction process, and the high temperature may cause the active ingredients contained in the Bordeaux leaf to be decomposed or denatured by heat, thereby resulting in the activity of the extracted Bordeaux leaf extract being low or even ineffective. Based on this, the preparation method of the Bordeaux leaf extract of some embodiments of the present disclosure first freezes and crushes the Bordeaux leaf to obtain a Bordeaux leaf powder. By freezing and crushing to obtain the Bordeaux leaf powder, it is beneficial to the subsequent full extraction of the extract. Secondly, the above-mentioned Bordeaux leaf powder is mixed with vegetable oil to obtain a first mixture. Since the vegetable oil itself contains certain bioactive ingredients, it can synergistically act with the active ingredients contained in the Bordeaux leaf to obtain a Bordeaux leaf extract with better activity. Then, the above-mentioned first mixture is mixed with an emulsifier to obtain a second mixture. By combining the emulsifier to promote the further extraction of the active ingredients. Further, the above-mentioned second mixture after standing is subjected to microwave extraction to obtain a first extract. In addition, the above-mentioned first extract is subjected to ultrasonic extraction to obtain a second extract. In addition to this, the above-mentioned second extract is subjected to suction filtration treatment to obtain a filtrate as a third extract. Finally, the above-mentioned third extract is subjected to centrifugal treatment to obtain a supernatant as a Bordeaux leaf extract. Through this method, compared with the extraction method based on the alcohol organic solvent, the Bordeaux leaf extract with better activity can be prepared. BRIEF DESCRIPTION OF DRAWINGS

[0019] The above and other features, advantages, and aspects of the embodiments of the present disclosure will become more apparent with reference to the following detailed description in conjunction with the accompanying drawings. Throughout the drawings, the same or similar reference numerals can refer to the same or similar elements. It should be understood that the drawings are schematic, and elements are not necessarily drawn to scale.

[0020] Figure 1 is a comparison chart of NO inhibition rates of different samples of some embodiments of the present disclosure;

[0021] Figure 2 is a comparison chart of relative expression amounts of COL-1 genes corresponding to different samples of some embodiments of the present disclosure;

[0022] Figure 3 is a comparison chart of relative expression amounts of MMP-1 genes corresponding to different samples of some embodiments of the present disclosure;

[0023] Figure 4 is a comparison chart of relative expression amounts of MMP-1 genes corresponding to another different sample of some embodiments of the present disclosure;

[0024] Figure 5 is a relative expression amount comparison chart of the gene of COL-1 corresponding to different samples of some embodiments of the present disclosure;

[0025] Figure 6 is a relative expression amount comparison chart of the gene of IL-6 corresponding to different samples of some embodiments of the present disclosure. DETAILED DESCRIPTION

[0026] Embodiments of the present disclosure will be described in more detail with reference to the drawings. Although certain embodiments of the present disclosure are shown in the drawings, it is understood that the present disclosure can be embodied by various forms and should not be interpreted as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that the present disclosure can be more thoroughly and completely understood. It should be understood that the drawings and embodiments of the present disclosure are only for exemplary purposes and are not intended to limit the scope of protection of the present disclosure.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Generally, the terms used herein are intended to have their ordinary and customary meaning to those skilled in the art. When amounts, concentrations, or other values or parameters are expressed in ranges, preferred ranges, or a series of upper preferred values and lower preferred values, it is understood that all ranges formed from any pair of an upper limit or an upper preferred value and a lower limit or a lower preferred value are expressly disclosed. Whether the range is expressly disclosed or not, when numerical ranges are described herein, unless otherwise stated, the range is intended to include the end values and all integers and fractions within that range.

[0028] It should also be noted that only parts related to the application are shown in the drawings for the convenience of description. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0029] It should be noted that the modification of "one" or "a plurality of" mentioned in the present disclosure is illustrative rather than restrictive, and those skilled in the art should understand that it should be understood as "one or more" unless otherwise explicitly indicated in the context.

[0030] The main terms used in the present disclosure are defined as follows:

[0031] The term "aging" includes a process with oxidative damage, energy failure or mitochondrial dysfunction. Symptoms of aging include, but are not limited to, wrinkles, water loss, loss of skin elasticity, and uneven pigmentation.

[0032] The term "jojoba oil", also known as "jojoba butter", is a natural oil extracted from a plant native to Mexico. The jojoba oil is light yellow in color. The jojoba oil has good skin affinity and can shrink pores, increase skin elasticity, delay aging, reduce fine lines, and repair sensitive skin.

[0033] The term "rice germ oil" is an oil extracted from rice germ.

[0034] The term "rapeseed oil" is a vegetable oil extracted from rapeseed. The rapeseed oil is transparent or translucent, and has a golden or brownish yellow color. The main components of rapeseed oil include: erucic acid, oleic acid, linoleic acid, linolenic acid, tocopherol, and rapeseed sterol.

[0035] The term "olive oil" is a vegetable oil extracted from the fruit of Olea europaea. The olive oil is light yellow in color. The olive oil has the effects of maintaining skin elasticity and moisturizing the skin.

[0036] The term "squalane", also known as isomerotriacontane, is a transparent oily viscous liquid prepared by hydrogenation of squalene. The squalane is colorless and odorless. In practice, squalane can also be extracted from olive oil, amaranth oil, palm oil, and other vegetable oils. Squalane has good skin affinity and can accelerate the penetration of active substances into the skin.

[0037] The term "sunflower oil", also known as "sunflower seed oil", is a vegetable oil extracted from sunflower seeds. The sunflower oil is transparent, and has a golden yellow color. The sunflower oil is rich in linoleic acid and also contains active substances such as vitamin E. The sunflower oil has good anti-inflammatory, antioxidant, and anti-aging effects.

[0038] The term "white pool flower oil" is a vegetable oil extracted from white pool flowers. The white pool flower oil has good antioxidant and moisturizing effects.

[0039] The method for preparing the bordeaux leaf extract of the present disclosure can include the following steps:

[0040] First, the bordeaux leaves are frozen and pulverized to obtain bordeaux leaf powder.

[0041] As an example, first, the bordeaux leaves can be frozen and solidified using liquid nitrogen rapid freezing. Then, the frozen and solidified bordeaux leaves are pulverized to a target mesh size using a frozen grinding instrument to obtain the bordeaux leaf powder. In practice, the target mesh size can be 40 mesh to 100 mesh. For example, the target mesh size can be 60 mesh. The frozen grinding instrument can be a SPEX 6875D high-throughput liquid nitrogen frozen grinding instrument.

[0042] As a further example, first, the bordeaux leaves can be subjected to low-temperature drying treatment to obtain dried bordeaux leaves. Then, the dried bordeaux leaves are crushed to a target mesh number to obtain the above-mentioned bordeaux leaf powder. In practice, the target mesh number can be 40 mesh to 100 mesh.

[0043] Secondly, the above-mentioned bordeaux leaf powder is mixed with plant oil to obtain a first mixture.

[0044] The mass of the plant oil is 3 times to 100 times the mass of the bordeaux leaf powder.

[0045] Optionally, the plant oil includes one or more of jojoba oil, rice germ oil, rapeseed oil, olive oil, squalane, sunflower oil, and white pool flower oil.

[0046] Thirdly, the above-mentioned first mixture is mixed with an emulsifier to obtain a second mixture.

[0047] The mass fraction of the emulsifier is 5% to 15% of the mass fraction of the first mixture.

[0048] Optionally, the emulsifier is a polyglycerin emulsifier. The above-mentioned emulsifier includes one or more of polyglyceryl-2 triisostearate, polyglyceryl-10 isostearate, polyglyceryl-2 isostearate, polyglyceryl-2 dipolyhydroxystearate, polyglyceryl-3 diisostearate, polyglyceryl-2 isostearate / dipolylinoleate copolymer, polyglyceryl-2 sesquiisocaprylate, and polyglyceryl-2 stearate.

[0049] Fourthly, the above-mentioned second mixture after standing is subjected to microwave extraction to obtain a first extract.

[0050] The above-mentioned second mixture after standing can be subjected to microwave extraction by using an ultrasonic-microwave catalytic extraction instrument to obtain the first extract. In practice, the ultrasonic-microwave catalytic extraction instrument can be an extraction instrument with ultrasonic extraction and microwave extraction (FCMCR-3C).

[0051] Optionally, before the above-mentioned second mixture after standing is subjected to microwave extraction to obtain the first extract, the above-mentioned method further includes: standing the above-mentioned second mixture at a standing temperature in the range of 4 degrees Celsius to 35 degrees Celsius for 24 hours to 72 hours to obtain the above-mentioned second mixture after standing.

[0052] Optionally, the above-mentioned microwave extraction on the second mixture after standing to obtain the first extract, comprising: using a microwave extraction power of 200 watts to 800 watts, the second mixture after standing to obtain the target number of cycles of microwave extraction, to obtain the first extract, wherein the target number is 2 to 4 times, the extraction time of a single cycle of microwave extraction is 5 minutes to 10 minutes, and the time interval between two consecutive cycles of microwave extraction is 2 minutes to 10 minutes.

[0053] The fifth step is to perform ultrasonic extraction on the first extract to obtain a second extract.

[0054] Optionally, the above-mentioned microwave extraction on the second mixture after standing to obtain the first extract, comprising: using a microwave extraction power of 200 watts to 800 watts, the second mixture after standing to obtain the target number of cycles of microwave extraction, to obtain the first extract, wherein the target number is 2 to 4 times, the extraction time of a single cycle of microwave extraction is 5 minutes to 10 minutes, and the time interval between two consecutive cycles of microwave extraction is 2 minutes to 10 minutes.

[0055] Optionally, the above-mentioned microwave extraction on the second mixture after standing to obtain the first extract, comprising: using a microwave extraction power of 200 watts to 800 watts, the second mixture after standing to obtain the target number of cycles of microwave extraction, to obtain the first extract, wherein the target number is 2 to 4 times, the extraction time of a single cycle of microwave extraction is 5 minutes to 10 minutes, and the time interval between two consecutive cycles of microwave extraction is 2 minutes to 10 minutes.

[0056] The sixth step is to perform filtration on the second extract to obtain a filtrate as a third extract.

[0057] As an example, a vacuum pump (SN-HP-02) can be used to perform filtration on the second extract to obtain a filtrate as a third extract.

[0058] The seventh step is to perform centrifugal treatment on the third extract to obtain a supernatant as a bordeaux leaf extract.

[0059] As an example, a centrifuge can be used to perform centrifugal treatment on the third extract to obtain a supernatant as a bordeaux leaf extract. In practice, a floor-standing high-speed centrifuge (Thermo, Thermo) can be used to perform centrifugal treatment on the third extract to obtain a supernatant as a bordeaux leaf extract.

[0060] Optionally, the above-mentioned microwave extraction on the second mixture after standing to obtain the first extract, comprising: using a microwave extraction power of 200 watts to 800 watts, the second mixture after standing to obtain the target number of cycles of microwave extraction, to obtain the first extract, wherein the target number is 2 to 4 times, the extraction time of a single cycle of microwave extraction is 5 minutes to 10 minutes, and the time interval between two consecutive cycles of microwave extraction is 2 minutes to 10 minutes.

[0061] Optionally, the above-mentioned sixth step to seventh step can be repeated 2-3 times, and the supernatant is collected as the above-mentioned extract of boronia leaf.

[0062] The extract of boronia leaf in the present disclosure can be used for preparing a skin care product. The skin care product is one or more of the following: serum, emulsion, cream, and cream.

[0063] The extract of boronia leaf in the present disclosure can be used for the purpose of anti-photoaging and repair of photo-damage.

[0064] The skin care product containing the extract of boronia leaf in the present disclosure can be used for the purpose of anti-photoaging and repair of photo-damage.

[0065] The present disclosure will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0066] Embodiment 1

[0067] In the embodiment 1, rapeseed oil is used as the plant oil, and polyglyceryl-2 triisostearate is used as the emulsifier to prepare the extract of boronia leaf.

[0068] Preparation method:

[0069] In the first step, the boronia leaf is frozen and crushed to obtain the boronia leaf powder.

[0070] In practice, 500 grams of boronia leaf is rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified boronia leaf is ground to 60 mesh by a frozen grinder to obtain the above-mentioned boronia leaf powder.

[0071] In the second step, the above-mentioned boronia leaf powder is mixed with the plant oil to obtain a first mixture.

[0072] In practice, the boronia leaf powder and the rapeseed oil are uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0073] In the third step, the above-mentioned first mixture is mixed with the emulsifier to obtain a second mixture.

[0074] In practice, 15% of polyglyceryl-2 triisostearate by mass fraction is added to the above-mentioned first mixture, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the above-mentioned second mixture.

[0075] In the fourth step, the above-mentioned second mixture after standing is subjected to microwave extraction to obtain a first extract.

[0076] In practice, the second mixture, after being left to stand, was subjected to microwave extraction for two cycles using an ultrasonic-microwave catalytic extractor to obtain the first extract. The microwave extraction power was 600 watts. The microwave irradiation time for each cycle was 6 minutes, with a 2-minute interval between cycles.

[0077] The fifth step involves ultrasonic extraction of the first extract to obtain the second extract.

[0078] In practice, the first extract was subjected to ultrasonic extraction using an ultrasonic-microwave catalytic extraction instrument to obtain the second extract. The ultrasonic extraction power was 160 watts, and the extraction time was 20 minutes.

[0079] The sixth step is to filter the second extract to obtain a filtrate, which is then used as the third extract.

[0080] In practice, the second extract is filtered using a vacuum pump to obtain the filtrate, which is then used as the third extract.

[0081] Step 7: Centrifuge the third extract to obtain a supernatant, which is used as the Bordeaux leaf extract.

[0082] In practice, the third extract is centrifuged using a floor-standing high-speed centrifuge to obtain a supernatant, which serves as the Bordeaux leaf extract. The floor-standing high-speed centrifuge operates at 4000 rpm for 10 minutes. Specifically, step seven can be repeated twice to obtain the Bordeaux leaf extract.

[0083] Example 2

[0084] In Example 2, rapeseed oil was used as the vegetable oil and polyglycerol-2 triisostearate was used as the emulsifier to prepare Bordeaux leaf extract.

[0085] Preparation method:

[0086] The first step is to freeze and pulverize the Bordeaux leaves to obtain Bordeaux leaf powder.

[0087] In practice, 500 grams of Bordeaux leaves are rapidly frozen and solidified using liquid nitrogen, and then the frozen and solidified Bordeaux leaves are ground to 60 mesh using a cryogenic grinder to obtain the aforementioned Bordeaux leaf powder.

[0088] The second step is to mix the Bordeaux leaf powder with vegetable oil to obtain the first mixture.

[0089] In practice, Bordeaux leaf powder and rapeseed oil are mixed evenly at a mass ratio of 1:30 to obtain the first mixture.

[0090] Thirdly, the first mixture is mixed with an emulsifier to obtain a second mixture.

[0091] In practice, the first mixture is added with 5% of polyglyceryl-2 triisostearate by mass fraction and soaked for 24 hours at a temperature of 25 degrees Celsius to obtain the second mixture.

[0092] Fourthly, the second mixture after standing is subjected to microwave extraction to obtain a first extract.

[0093] In practice, the second mixture after standing is subjected to microwave extraction for 2 cycles by an ultrasonic-microwave catalytic extraction instrument to obtain the first extract. The microwave extraction power is 600 watts. The microwave irradiation time of each cycle is 6 minutes. The cycle interval is 2 minutes.

[0094] Fifthly, the first extract is subjected to ultrasonic extraction to obtain a second extract.

[0095] In practice, the first extract is subjected to ultrasonic extraction by an ultrasonic-microwave catalytic extraction instrument to obtain the second extract. The ultrasonic extraction power is 160 watts. The ultrasonic extraction time is 20 minutes.

[0096] Sixthly, the second extract is subjected to filtration treatment to obtain a filtrate as a third extract.

[0097] In practice, the second extract is subjected to filtration treatment by a vacuum pump to obtain a filtrate as the third extract.

[0098] Seventhly, the third extract is subjected to centrifugal treatment to obtain a supernatant as a bordeaux leaf extract.

[0099] In practice, the third extract is subjected to centrifugal treatment by a floor type high-speed centrifuge to obtain a supernatant as the bordeaux leaf extract. The centrifuge speed of the floor type high-speed centrifuge is 4000 rpm, and the centrifugation time is 10 minutes. Specifically, the execution subject can repeat the seventh step for 2 times to obtain the bordeaux leaf extract.

[0100] Example 3

[0101] In the example 3, the bordeaux leaf extract is prepared by using rapeseed oil as the plant oil and polyglyceryl-10 isostearate as the emulsifier.

[0102] Preparation method:

[0103] Firstly, the bordeaux leaves are frozen and crushed to obtain bordeaux leaf powder.

[0104] In practice, 500 grams of the bordeaux leaves are rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified bordeaux leaves are ground to 60 mesh by a freeze grinder to obtain the bordeaux leaf powder.

[0105] In practice, 500 grams of the bordeaux leaves are rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified bordeaux leaves are ground to 60 mesh by a freeze grinder to obtain the bordeaux leaf powder.

[0106] In practice, the bordeaux leaf powder and the rapeseed oil are uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0107] In practice, the bordeaux leaf powder and the rapeseed oil are uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0108] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0109] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0110] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0111] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0112] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0113] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0114] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0115] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0116] In practice, the polyglyceryl-10 isostearate is added to the first mixture at a mass fraction of 15%, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0117] Comparative Example 1

[0118] In Comparative Example 1, the bordeaux leaf extract was prepared by using rapeseed oil as the vegetable oil and sorbitan oleate as the emulsifier. Sorbitan oleate is commonly used in cosmetics, food, and other fields, and can be used in the preparation of food, oral and topical drugs. It is generally considered to be non-toxic and non-irritating.

[0119] Preparation method:

[0120] Step 1: Freeze and crush the bordeaux leaves to obtain bordeaux leaf powder.

[0121] In practice, 500 grams of bordeaux leaves were rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified bordeaux leaves were ground to 60 mesh by a freeze grinder to obtain the above-mentioned bordeaux leaf powder.

[0122] Step 2: Mix the above-mentioned bordeaux leaf powder with vegetable oil to obtain a first mixture.

[0123] In practice, the bordeaux leaf powder and rapeseed oil were uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0124] Step 3: Mix the above-mentioned first mixture with an emulsifier to obtain a second mixture.

[0125] In practice, 15% by mass of sorbitan oleate was added to the above-mentioned first mixture and soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the above-mentioned second mixture.

[0126] Step 4: Microwave extract the above-mentioned second mixture after standing to obtain a first extract.

[0127] In practice, the above-mentioned second mixture after standing was microwave extracted for 2 cycles by an ultrasonic-microwave catalytic extraction instrument to obtain the first extract. The microwave extraction power was 600 watts. The microwave irradiation time for each cycle was 6 minutes. The cycle interval was 2 minutes.

[0128] Step 5: Ultrasonic extract the above-mentioned first extract to obtain a second extract.

[0129] In practice, the above-mentioned first extract was ultrasonic extracted by an ultrasonic-microwave catalytic extraction instrument to obtain the second extract. The ultrasonic extraction power was 160 watts. The ultrasonic extraction time was 20 minutes.

[0130] Step 6: Perform suction filtration on the above-mentioned second extract to obtain a filtrate as a third extract.

[0131] In practice, the second extract is subjected to filtration by a vacuum pump to obtain a filtrate as a third extract.

[0132] In practice, the third extract is subjected to centrifugal treatment by a floor type high-speed centrifuge to obtain a supernatant as the extract of the leaves of the bordeaux tree. The centrifuge speed of the floor type high-speed centrifuge is 4000 rpm, and the centrifugal time is 10 minutes. Specifically, the execution subject can repeatedly execute the seventh step for 2 times to obtain the extract of the leaves of the bordeaux tree.

[0133] In practice, the third extract is subjected to centrifugal treatment by a floor type high-speed centrifuge to obtain a supernatant as the extract of the leaves of the bordeaux tree. The centrifuge speed of the floor type high-speed centrifuge is 4000 rpm, and the centrifugal time is 10 minutes. Specifically, the execution subject can repeatedly execute the seventh step for 2 times to obtain the extract of the leaves of the bordeaux tree.

[0134] Comparative Example 2

[0135] In the comparative example 2, the extract of the leaves of the bordeaux tree is prepared by using rapeseed oil as the vegetable oil and using polysorbate-20 as the emulsifier. The polysorbate-20, also known as Tween 20, is a yellowish to yellow viscous liquid at room temperature. It is commonly used in the fields of food, cosmetics, etc.

[0136] Preparation method:

[0137] In practice, 500 grams of the leaves of the bordeaux tree are rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified leaves of the bordeaux tree are ground to 60 mesh by a frozen grinder to obtain the leaf powder of the bordeaux tree.

[0138] In practice, 500 grams of the leaves of the bordeaux tree are rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified leaves of the bordeaux tree are ground to 60 mesh by a frozen grinder to obtain the leaf powder of the bordeaux tree.

[0139] In practice, 500 grams of the leaves of the bordeaux tree are rapidly frozen and solidified by liquid nitrogen, and the frozen and solidified leaves of the bordeaux tree are ground to 60 mesh by a frozen grinder to obtain the leaf powder of the bordeaux tree.

[0140] In practice, the leaf powder of the bordeaux tree and the rapeseed oil are uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0141] In practice, the leaf powder of the bordeaux tree and the rapeseed oil are uniformly mixed at a mass ratio of 1:30 to obtain the first mixture.

[0142] In practice, 15% of polysorbate-20 by mass fraction is added to the first mixture, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0143] In practice, 15% of polysorbate-20 by mass fraction is added to the first mixture, and the mixture is soaked at a temperature of 25 degrees Celsius for 24 hours to obtain the second mixture.

[0144] In practice, the second mixture, after being left to stand, was subjected to microwave extraction for two cycles using an ultrasonic-microwave catalytic extractor to obtain the first extract. The microwave extraction power was 600 watts. The microwave irradiation time for each cycle was 6 minutes, with a 2-minute interval between cycles.

[0145] The fifth step involves ultrasonic extraction of the first extract to obtain the second extract.

[0146] In practice, the first extract was subjected to ultrasonic extraction using an ultrasonic-microwave catalytic extraction instrument to obtain the second extract. The ultrasonic extraction power was 160 watts, and the extraction time was 20 minutes.

[0147] The sixth step is to filter the second extract to obtain a filtrate, which is then used as the third extract.

[0148] In practice, the second extract is filtered using a vacuum pump to obtain the filtrate, which is then used as the third extract.

[0149] Step 7: Centrifuge the third extract to obtain a supernatant, which is used as the Bordeaux leaf extract.

[0150] In practice, the third extract is centrifuged using a floor-standing high-speed centrifuge to obtain a supernatant, which serves as the Bordeaux leaf extract. The floor-standing high-speed centrifuge operates at 4000 rpm for 10 minutes. Specifically, step seven can be repeated twice to obtain the Bordeaux leaf extract.

[0151] Comparative Example 3

[0152] In Comparative Example 3, no emulsifier or vegetable oil was used to prepare Bordeaux leaf extract. Furthermore, the preparation method of Comparative Example 3 differed from that of Examples 1, 2, 3, Comparative Example 1, and Comparative Example 2.

[0153] Preparation method:

[0154] The first step is to freeze and pulverize the Bordeaux leaves to obtain Bordeaux leaf powder.

[0155] In practice, 500 grams of Bordeaux leaves are dried at a constant temperature until they reach a constant weight, and then ground to a fine powder (60 mesh) to obtain the Bordeaux leaf powder described above. The constant temperature drying process is conducted at 45 degrees Celsius for 24 to 48 hours. Specifically, the constant temperature drying process can be stopped once the Bordeaux leaves have reached a constant weight.

[0156] The second step involves adding distilled water to the Bordeaux leaf powder and heating it in a water bath at 80 degrees Celsius for 2 hours under reflux, collecting the supernatant.

[0157] The ratio of the bordeaux leaf powder to the distilled water is 1:30. In practice, the supernatant can be collected for 3 times.

[0158] In the third step, the supernatant is concentrated to 1 / 3 of the original volume by a rotary evaporator to obtain the bordeaux leaf extract.

[0159] In practice, the supernatant is concentrated to 1 / 3 of the original volume by a rotary evaporator (N-1100D-WD) to obtain the bordeaux leaf extract.

[0160] Comparative Example 4

[0161] In the comparative example 4, no emulsifier and no vegetable oil are used for preparing the bordeaux leaf extract. In addition, the preparation method of the comparative example 4 is different from the preparation methods of the example 1, the example 2, the example 3, the comparative example 1 and the comparative example 2.

[0162] Preparation method:

[0163] In the first step, the bordeaux leaves are frozen and crushed to obtain the bordeaux leaf powder.

[0164] In practice, 500 grams of the bordeaux leaves are dried at a constant temperature until the constant weight, and the bordeaux leaves are crushed and ground to 60 mesh to obtain the bordeaux leaf powder. The temperature for the constant temperature drying is 45 degrees Celsius, and the constant temperature drying time is 24 hours to 48 hours. Specifically, when the bordeaux leaves are dried at a constant temperature until the constant weight, the constant temperature drying can be stopped.

[0165] In the second step, the bordeaux leaf powder is added with the ethanol aqueous solution with a volume fraction of 95%, and heated in a water bath at a temperature of 80 degrees Celsius for 1 hour to obtain the mixture.

[0166] The ratio of the bordeaux leaf powder to the ethanol aqueous solution is 1:30.

[0167] In the third step, the mixture is centrifuged to obtain the supernatant.

[0168] In practice, the mixture can be centrifuged for 3 times to obtain the supernatant. The centrifugal speed of the floor type high-speed centrifuge is 4000 rpm, and the centrifugal time is 15 minutes.

[0169] In the fourth step, the supernatant is concentrated to 1 / 3 of the original volume by a rotary evaporator to obtain the bordeaux leaf extract.

[0170] In practice, a rotary evaporator (N-1100D-WD) is selected to concentrate the supernatant to 1 / 3 of the original volume, thereby obtaining the above-mentioned Bordeaux leaf extract.

[0171] The polyphenol content and antioxidant effect of the Bordeaux leaf extract obtained according to the above examples and comparative examples will be determined and described in detail as follows.

[0172] Among them, the polyphenol content of sample 1, sample 2, sample 3, sample 3, sample 5, sample 6 and sample 7 is determined by Folin-Ciocalteu colorimetric method (colorimetric method). For detailed description of sample 1 to sample 7, see Table 1 below:

[0173] Table 1

[0174]

[0175] ABTS antioxidant assay

[0176] Among them, ABTS is oxidized by active oxygen to generate stable blue-green cation radical ABTS+·, and the maximum absorption wavelength is 734 nm (nanometer). The antioxidant components in sample 1 to sample 7 react with free radicals ABTS+·, which will cause the reaction system to fade. By detecting the change of absorbance, the clearance rate of the measured substance to free radicals ABTS+·, i.e. the total antioxidant capacity, can be directly calculated.

[0177] ABTS antioxidant assay preparation

[0178] Among them, the solution preparation: free radical ABTS+· stock solution, sample 1 to sample 7 solution.

[0179] ABTS antioxidant assay steps:

[0180] First, in group A1, 20 μL of sample and 180 μL of ABTS+· stock solution are added to each well.

[0181] Among them, the solution of sample 1 to sample 7 can be mixed with 180 μL of ABTS+· stock solution and then added to the multi-well plate.

[0182] Second, in group A2, 20 μL of sample and 180 μL of distilled water are added to each well.

[0183] Among them, the solution of sample 1 to sample 7 can be mixed with 180 μL of distilled water and then added to the multi-well plate.

[0184] Third, in group A0, 20 μL of distilled water and 180 μL of ABTS+· stock solution are added to each well.

[0185] In which, the distilled water and 180 μL distilled water can be added into the multi-well plate.

[0186] In which, the scavenging rate of the measured substance on free radical ABTS+· is [1-(A1-A2) / A0]x100%.

[0187] The scavenging rate of samples 1~7 on free radical ABTS+· and the polyphenol content are as follows in Table 2:

[0188] Table 2

[0189]

[0190] It can be seen that, first, the polyphenol content of the Bordeaux leaf extract prepared according to the preparation method of Example 1, Example 2 and Example 3 is higher than that of the Bordeaux leaf extract prepared according to the preparation method of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 3. Secondly, according to the verification results of ABTS antioxidant assay, the Bordeaux leaf extract prepared according to the preparation method of Example 1, Example 2 and Example 3 has better antioxidant capacity than the Bordeaux leaf extract prepared according to the preparation method of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 3.

[0191] Nitric oxide level determination

[0192] Nitric oxide level determination process: by setting blank control group, positive control group and experimental group. Specifically, HaCat cells were seeded in a 12-well plate at a density of 2x10 5 cells per milliliter (counting instrument counting) and incubated in an incubator overnight. Among them, no LPS (Lipopolysaccharide, Lipopolysaccharide) was added in the blank control group. The positive control group added 100 μg / L dexamethasone after 24 hours, and further incubated for 2 hours before adding 125 ng / mL LPS. The experimental group added samples as shown in Table 3 after 24 hours, and further incubated for 1 hour before adding 125 ng / mL LPS. Among them, (HaCat cells (Hacat human immortalized epidermal cells), batch number CC-Y1177, provided by Shanghai Zerene Biotechnology Co., Ltd.).

[0193] Table 3

[0194]

[0195] After incubating the blank control group, the positive control group and the experimental group for 24 hours, 50 μL of supernatant was taken in a 96-well plate. Among them, 50 μL of Griess reagent was added to each well, and after further incubation for 30 minutes, the absorbance at 540 nm wavelength was detected by an enzyme-labeled instrument. The NO content secreted by macrophages in each group was measured, and the corresponding inhibition rate of each group was calculated according to the following formula: inhibition rate (%) = (1-T / C) x 100%. Wherein, T represents the average value of the inflammatory factor content in the test sample. C represents the average value of the negative control inflammatory factor content. The comparison chart of NO inhibition rates of different samples is shown in Figure 1 Fig. 1, wherein Figure 1 The corresponding NO inhibition rates of only 100 μg / ul of dexamethasone, sample 1-1, sample 1-2, sample 2-1, sample 2-2, sample 3-1, sample 3-2, sample 4-1 and sample 4-2 are shown. It can be seen from the comparison that, compared with the bordeaux leaf extract prepared by the preparation method of Comparative Example 4, the bordeaux leaf extract prepared by the preparation methods of Example 1, Example 2 and Example 3 can play a significant role in inhibiting the content of NO. It shows that the bordeaux leaf extract obtained by using the polyglycerol emulsifier of the present disclosure has a good effect on inhibiting oxidative inflammation induced by LSP. Specifically, by comparing sample 1-1, sample 1-2, sample 2-1, sample 2-2, sample 3-1 and sample 3-2, the bordeaux leaf extract prepared by using polyglyceryl-2 triisostearate as an emulsifier has a better effect on inhibiting oxidative inflammation induced by LPS.

[0196] Light damage test

[0197] The light damage test process is as follows: blank control group, positive control group and experimental group are set. Specifically, 3T3 cell suspension at logarithmic phase is seeded in a 6-well plate at a density of 7 x 10 5 cells / well, and incubated for 24 hours. Among them, (3T3 cells (NIH / 3T3 mouse embryo cells), batch number CC-Y2070, provided by Shanghai Zymo Biological Technology Co., Ltd.).

[0198] The blank control group was discarded after 24 hours, and 1 milliliter of PBS (Phosphate buffered saline, phosphate buffered physiological saline) was added to each well of the 6-well plate. The positive control group was discarded after 24 hours, and the experimental group was irradiated with a UVB dose of 50 mJ / cm 2 The experimental group was discarded after 24 hours, and 1 milliliter of PBS was added to each well of the 6-well plate, and irradiated with a UVB dose of 50 mJ / cm 2The UVB dose of the UV irradiation was performed on the experimental groups, and after the irradiation, the PBS was discarded and the samples in Table 4 were added for sample loading.

[0199] Table 4

[0200]

[0201] After further culturing for 24 hours, the cells were collected by Trans Zol reagent. The collected cells were subjected to RNA extraction according to the instructions of the full-size gold ER501 Trans Zol Up Plus RNA Kit, and the total RNA concentration and purity were determined using a ultramicro UV-visible spectrophotometer. Reverse transcription was performed using a reverse transcription mix, and finally cDNA was synthesized. Then, qPCR detection was performed to detect the gene expression of β-actin and MMP-1, COL-1.

[0202] Further, the Ct values of β-actin and Collagen I, MMP-1 corresponding to the experimental groups of sample 1, sample 2, sample 3 and sample 3 were calculated, and the 2-△△Ct method was used to analyze the mRNA expression level of the related genes. Among them, the data between groups was analyzed by TTEST statistical analysis, and P<0.05 indicated that the difference was significant and had statistical significance.

[0203] Among them, the calculation formula of the 2-△△Ct method is as follows: △Ct=Ct1-Ct2; △△Ct=△Ct1-△Ct2; the relative expression amount of mRNA=2^(-△△Ct). Among them, Ct1 represents the Ct value of the target gene. Ct2 represents the Ct value of β-actin in the experimental group. △Ct1 represents the average value of the Ct value of the blank group. △Ct2 represents the average value of the Ct value of the experimental group. Specifically, the relative expression amount of COL-1 gene corresponding to different samples is as shown in Table 5, and the relative expression amount of MMP-1 gene corresponding to different samples is as shown in Table 6. Figure 2 Figure 3 ​As shown, by comparison, first, sample 1, sample 2 and sample 3 after UV irradiation can promote the production of COL-1 in fibroblasts, and reduce the content of MMP-1. It shows that the preparation method of example 1, example 2, example 3 for preparing bordeaux leaf extract can effectively promote the repair after light damage, and anti-aging effect. Secondly, by comparing sample 1, sample 2, sample 3 and sample 4, it is found that the bordeaux leaf extract prepared by the preparation method of example 1, example 2, example 3 for preparing bordeaux leaf extract has more excellent effect of promoting light damage repair and anti-aging. In addition, by comparing sample 1, sample 2 and sample 3, it is found that sample 1 and sample 2 prepared by using polyglycerol-2 triisostearate as emulsifier have better effect of promoting the production of COL-1 in fibroblasts and reducing the content of MMP-1, that is, the bordeaux leaf extract prepared by using polyglycerol-2 triisostearate as emulsifier has more excellent effect of promoting repair after light damage, anti-aging.

[0204] Light protection test

[0205] In the light protection test process: set up blank control group, positive control group and experimental group. Specifically, the logarithmic phase of 3T3 cell suspension was inoculated in 6-hole plate at the density of 7x10 5 / hole, and incubated for about 24 hours.

[0206] In which, the blank control group is not treated with UVB and no extract is added. The positive control group is treated with UVB and no extract is added. The experimental group is according to the sample shown in table 5, and is treated with UVB.

[0207] Table 5

[0208]

[0209] First, extract RNA by full type gold ER501 Trans Zol Up Plus RNA Kit instruction, and determine the total RNA concentration and purity by ultramicro ultraviolet-visible spectrophotometer, and use reverse transcription Mix for reverse transcription, and finally synthesize cDNA. Then, qPCR detection is carried out to detect the gene expression of β-actin and IL-6, MMP-1, COL-1.

[0210] Further, the Ct values of the experimental group corresponding to β-actin and Collagen I, MMP-1, IL-6 of the experimental sample group samples 1-1, sample 1-2, sample 2-1, sample 2-2, sample 3-1, sample 3-2, sample 4-1, sample 4-2 are calculated, and the 2-△△Ct method is used to analyze the mRNA expression level of the related genes, and the data between groups is analyzed by TTEST, P<0.05 indicates that the difference is significant and has statistical significance.

[0211] Wherein, the calculation formula of 2-△△Ct method is as follows: △Ct=Ct1-Ct2; △△Ct=△Ct1-△Ct2; the relative expression amount of mRNA=2^(-△△Ct). Wherein, Ct1 represents the Ct value of the target gene. Ct2 represents the Ct value of the experimental group β-actin. △Ct1 represents the average Ct value of the blank group. △Ct2 represents the average Ct value of the experimental group. Specifically, the relative expression amount of MMP-1 gene corresponding to different samples is as shown in Figure 4 , the relative expression amount of COL-1 gene corresponding to different samples is as shown in Figure 5 , and the relative expression amount of IL-6 gene corresponding to different samples is as shown in Figure 6 It can be seen by comparison that first, after UV irradiation, samples 1-1, sample 1-2, sample 2-1, sample 2-2, sample 3-1, sample 3-2, sample 4-1 and sample 4-2 can all inhibit the generation of collagen in photoaging-induced fibroblasts and the large production of MMPs and IL-6 induced by photoaging. It shows that the extract of portugal tree leaves can effectively protect fibroblasts from light damage, that is, the extract of portugal tree leaves has the effects of anti-photoaging, anti-aging and anti-inflammatory. Second, by comparing sample 1-1, sample 1-2, sample 2-1, sample 2-2, sample 3-1 and sample 3-2, it is found that sample 1-2 prepared by using polyglycerol-2 triisostearate as an emulsifier has better effects of inhibiting the generation of collagen in photoaging-induced fibroblasts and inhibiting the large production of MMPs and IL-6 induced by photoaging, and presents a certain concentration dependence, that is, the extract of portugal tree leaves prepared by using polyglycerol-2 triisostearate as an emulsifier has more excellent effects of anti-photoaging, anti-aging and anti-inflammatory.

[0212] The use of the Bordeaux leaf extract described above in anti-photoaging and photodamage repair solves the second technical problem mentioned in the background art, i.e. "the lack of effective skin repair and protection methods from the cause level, especially the lack of skin repair and protection methods from the photo-cause level, resulting in the need for improved skin repair and protection effects". In practice, conventional repair is often symptomatic adaptive skin repair, and there is a lack of skin repair and protection methods from the photo-cause level. Through the Bordeaux leaf extract with more active substances of the present disclosure, skin problems induced by photoaging can be eliminated from the anti-photoaging and photodamage level, i.e. the source, and at the same time, skin repair and protection can be performed for the already generated photoaging and photodamage problems.

[0213] The above description is merely some preferred embodiments of the present disclosure and an explanation of the principles of the technology used. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the technical features described above, and should also cover other technical solutions formed by any combination of the above technical features or equivalent features without deviating from the above inventive concept. For example, the above features are replaced with each other to form technical solutions with similar functions to the technical features disclosed in the embodiments of the present disclosure (but not limited to).

Claims

1. A method for preparing Bordeaux leaf extract for skin anti-photoaging and photodamage repair, comprising: Bordeaux leaves were frozen and pulverized to obtain Bordeaux leaf powder; The Bordeaux leaf powder was mixed with vegetable oil to obtain a first mixture, wherein the vegetable oil was rapeseed oil. The first mixture is mixed with an emulsifier to obtain a second mixture, wherein the emulsifier is polyglycerol-2 triisostearate or polyglycerol-10 isostearate; The second mixture, after being allowed to stand, was subjected to microwave extraction to obtain the first extract; The first extract was subjected to ultrasonic extraction to obtain a second extract; The second extract is subjected to vacuum filtration to obtain a filtrate, which is used as the third extract. The third extract was centrifuged to obtain a supernatant, which was used as the Bordeaux leaf extract.

2. The preparation method according to claim 1, wherein, Before performing microwave extraction on the settled second mixture to obtain the first extract, the method further includes: The second mixture is left to stand for 24 to 72 hours within a standing temperature range of 4°C to 35°C to obtain the second mixture after standing.

3. The preparation method according to claim 1, wherein, The microwave extraction of the second mixture after it has been left to stand yields a first extract, comprising: The second mixture after settling is subjected to a target number of microwave extraction cycles at a microwave extraction power of 200 watts to 800 watts to obtain the first extract. The target number of extraction cycles is 2 to 4, the extraction time of a single microwave extraction cycle is 5 to 10 minutes, and the time interval between two consecutive microwave extraction cycles is 2 to 10 minutes.

4. The preparation method according to claim 1, wherein, The process of ultrasonically extracting the first extract to obtain the second extract includes: The first extract is subjected to a target number of ultrasonic extraction cycles at an ultrasonic extraction power of 100 watts to 300 watts to obtain the second extract. The target number of cycles is 2 to 4, and the extraction time for each cycle of ultrasonic extraction is 15 to 30 minutes.

5. The preparation method according to claim 1, wherein, The centrifugation of the third extract to obtain a supernatant, as a Bordeaux leaf extract, comprises: The third extract was centrifuged at a temperature of 4°C to 20°C and a speed of 3600 rpm to 5000 rpm for 5 to 15 minutes, and the supernatant was collected as the Bordeaux leaf extract.

6. A skin care product for anti-photoaging and photodamage repair of skin, made from Bordeaux leaf extract prepared by any one of claims 1-5.

7. The use of the Bordeaux leaf extract prepared by any one of claims 1-5 in the preparation of products for skin anti-photoaging and photodamage repair.

8. Use of the skin care product according to claim 6 in the preparation of products for skin anti-photoaging and photodamage repair.

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