A method for preparing animal-derived amino acid protein peptides
By performing two enzymatic hydrolysis and fermentation processes on bovine bones, combined with two acid decomposition processes, the problems of low amino acid content and poor binding force in existing technologies have been solved. This has enabled the effective combination of amino acids and trace elements, thereby enhancing the nutritional value of protein peptides.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUIZHOU XINHUA BIOTECH DEV CO LTD
- Filing Date
- 2024-05-17
- Publication Date
- 2026-06-30
AI Technical Summary
The existing technology has low peptone amino acid content, large peptide chain molecules, and poor binding force with other elements, resulting in insufficient nutritional value.
The process involves two enzymatic hydrolysis and fermentation processes using bovine bones, combined with two acid decomposition methods, to promote the formation of amino acids and the binding of trace elements, thereby increasing the content of amino acids and small molecule peptides.
It increases the content of amino acids and trace elements, allowing them to effectively combine with amino acids and enhance the nutritional value of the product.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of protein peptide preparation technology, and in particular to a method for preparing animal-derived amino acid protein peptides. Background Technology
[0002] Reagent-grade peptone is a peptone product used as a laboratory reagent in fields such as biochemistry and medicine, and has now become an indispensable raw material for preparing microbial culture media. Current peptone technology uses protamine as a matrix and is prepared as a dried powder through special hydrolysis extraction. It is rich in nutrients and provides a nitrogen source for microbial growth in culture media, making it suitable for use as a raw material for culture media and for the production of engineered microbial bacteria.
[0003] However, existing peptones have low amino acid content, large peptide chains, and poor binding affinity with other elements. To promote the formation of various amino acids and the synthesis of beneficial elements, it is necessary to improve the preparation process and develop a method for producing amino acid protein peptides. Summary of the Invention
[0004] In view of this, the present invention provides a method for preparing animal-derived amino acid protein peptides, which involves two enzymatic hydrolysis and fermentation processes on bovine bones, combined with two acid decomposition processes, to promote the formation of amino acids, increase the amino acid content, improve the content of trace elements, and effectively combine them with amino acids to enhance the nutritional value of the product.
[0005] The method for preparing the animal-derived amino acid protein peptide includes the following steps:
[0006] (1) Raw material enzymatic hydrolysis: After sterilizing the beef bones, add 30% water and steam at 200-220℃ and 4-5MPa for 4-5 hours. Remove bone residue and floating oil to obtain beef bone cooking soup. Then adjust the pH to 7.0-8.0, add 6-8% trypsin of the total weight of the raw materials and carry out enzymatic hydrolysis at 40-45℃ to obtain the first enzymatic hydrolysate.
[0007] (2) Primary fermentation: The first enzymatic hydrolysate obtained in step (1) is fermented, precipitated, and filtered to obtain the first fermentation liquid;
[0008] (3) Secondary enzymatic hydrolysis: Add trypsin to the first fermentation broth described in step (2) and enzymatically hydrolyze at 40-45℃ for 8-10 hours to obtain the second enzymatic hydrolysate;
[0009] (4) Primary acid decomposition: Add sulfuric acid solution to the second enzymatic hydrolysate and decompose acid at 100℃ to obtain sulfuric acid decomposition solution;
[0010] (5) Secondary fermentation: Filter the sulfuric acid decomposition liquid to remove impurities, add trypsin at 40-50℃, and keep it at 40-45℃ for 8-10 hours to obtain the second fermentation liquid;
[0011] (6) Secondary acid decomposition: Add hydrochloric acid solution to the second fermentation broth and decompose the acid at 100℃ to obtain hydrochloric acid decomposition broth;
[0012] (7) The hydrochloric acid decomposition solution was filtered to remove impurities, concentrated at low temperature, and spray-dried to obtain the animal-derived amino acid protein peptide.
[0013] Preferably, the fermentation time in step (2) is 8 to 10 hours and the fermentation temperature is 45°C; during the fermentation process, 20% to 25% of the weight of the first enzymatic hydrolysate of trypsin is added.
[0014] Preferably, the amount of trypsin added in step (3) is 20-22% of the weight of the first fermentation broth, and the fermentation time is 8-10 hours.
[0015] Preferably, the sulfuric acid solution in step (4) has a pH of 2, is added in an amount of 20-22% of the weight of the second enzymatic hydrolysate, and the acid decomposition time is 10-12 hours.
[0016] Preferably, the amount of trypsin added in step (5) is 10-12% of the weight of the sulfuric acid decomposition solution, and the fermentation time is 10-12 hours.
[0017] Preferably, the hydrochloric acid solution in step (6) has a pH of 4, is added in an amount of 10-12% of the weight of the second fermentation broth, and the acid decomposition time is 8-9 hours.
[0018] Preferably, the low-temperature vacuum concentration in step (7) involves filtering the hydrochloric acid decomposition solution to remove impurities and concentrating it to 70% at 60-70°C.
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] This invention provides a method for preparing animal-derived amino acid protein peptides, which involves two enzymatic hydrolysis and fermentation processes of bovine bones, combined with two acid decomposition processes, to promote the formation of amino acids and increase the content of amino acids and small molecule peptides. Furthermore, the two acid decomposition processes in this application increase the content of free trace elements in the product, enabling them to effectively combine with amino acids and improve the nutritional value of the product. Detailed Implementation
[0021] The present invention will be further described below with reference to the embodiments.
[0022] Example 1
[0023] A method for preparing animal-derived amino acid protein peptides, comprising the following steps:
[0024] (1) Raw material enzymatic hydrolysis: After disinfecting the beef bones, add water at 30% of the weight of the beef bones and cook at 210℃ and 4MPa for 4 hours. Remove bone residue and floating oil to obtain beef bone cooking soup. Then adjust the pH to 7.0, add trypsin at 8% of the total weight of the raw materials and enzymatically hydrolyze at 45℃ for 8 hours to obtain the first enzymatic hydrolysate.
[0025] (2) Primary fermentation: The first enzymatic hydrolysate obtained in step (1) is fermented, precipitated, and filtered to obtain the first fermentation liquid; the fermentation time is 8 hours and the fermentation temperature is 45℃; 20% of the weight of the first enzymatic hydrolysate of trypsin is added during the fermentation process.
[0026] (3) Secondary enzymatic hydrolysis: Add 20% trypsin to the first fermentation broth in step (2) and enzymatically hydrolyze at 45°C for 8 hours to obtain the second enzymatic hydrolysate; the amount of trypsin added is 20% of the weight of the first fermentation broth, and the fermentation time is 12 hours;
[0027] (4) First acid decomposition: Add sulfuric acid solution to the second enzymatic hydrolysate and decompose acid at 100°C to obtain sulfuric acid decomposition solution; the pH value of the sulfuric acid solution is 2, the amount added is 20% of the weight of the second enzymatic hydrolysate, and the acid decomposition time is 12 hours.
[0028] (5) Secondary fermentation: The sulfuric acid decomposition liquid is filtered to remove impurities, and trypsin is added at 50°C and fermented at 45°C for 10 hours to obtain the second fermentation liquid; the amount of trypsin added is 10% of the weight of the sulfuric acid decomposition liquid, and the fermentation time is 12 hours.
[0029] (6) Secondary acid decomposition: Hydrochloric acid solution is added to the second fermentation broth and acid decomposition is carried out at 100°C to obtain hydrochloric acid decomposition solution; the pH value of the hydrochloric acid solution is 4, the amount added is 10% of the weight of the second fermentation broth, and the acid decomposition time is 8 hours.
[0030] (7) The hydrochloric acid decomposition solution was filtered to remove impurities, concentrated to 70% at 60-70℃, and spray-dried to obtain the animal-derived amino acid protein peptide.
[0031] The animal-derived amino acid protein peptides prepared in Example 1 were subjected to component analysis.
[0032] The amino acid content is shown in Table 1:
[0033] Table 1
[0034]
[0035]
[0036] The trace element content is shown in Table 2:
[0037] Table 2
[0038]
[0039]
[0040] Comparative Example 1
[0041] A method for preparing an animal-derived amino acid protein peptide, the steps of which are as described in Example 1:
[0042] The difference is that Comparative Example 1 only performs steps (1) and (2), then removes impurities from the first fermentation broth, concentrates it to 70% at 60-70℃, and spray-dries it to obtain the animal-derived amino acid protein peptide.
[0043] The trace elements and amino acids in Comparative Example 1 were detected, and their contents were extremely low, proving that the method of this application improves the content of small molecules.
[0044] The results of tests performed on Example 1 and Comparative Example 1 are shown in Table 3:
[0045] Table 3
[0046] Inspection items Standard requirements Example 1 Comparative Example 1 Amino nitrogen ≥4％ 4.6％ 2.56％ Total phosphorus ≤0.3％ 0.13％ 0.13％ chloride ≤3％ 1.3％ 1.3 Ash ≤7％ 4.8％ 5.6
[0047] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for preparing animal-derived amino acid protein peptides, characterized in that, Includes the following steps: (1) Raw material enzymatic hydrolysis: After sterilizing the beef bones, add 30% water and steam at 200~220℃ and 4~5MPa for 4~5 hours. Remove bone residue and floating oil to obtain beef bone steaming soup. Then adjust the pH to 7.0~8.0, add 6~8% trypsin of the total weight of the raw materials and carry out enzymatic hydrolysis at 40~45℃ to obtain the first enzymatic hydrolysate. (2) Primary fermentation: The first enzymatic hydrolysate obtained in step (1) is fermented, precipitated, and filtered to obtain the first fermentation liquid; (3) Secondary enzymatic hydrolysis: Add trypsin to the first fermentation broth described in step (2) and enzymatically hydrolyze at 40-45℃ for 8-10 hours to obtain the second enzymatic hydrolysate; (4) First acid decomposition: Add sulfuric acid solution to the second enzymatic hydrolysate and decompose at 100℃ to obtain sulfuric acid decomposition solution; (5) Secondary fermentation: Filter the sulfuric acid decomposition liquid to remove impurities, add trypsin at 40~50℃, and keep it at 40~45℃ for 8~10h to obtain the second fermentation liquid; (6) Secondary acid decomposition: Add hydrochloric acid solution to the second fermentation broth and decompose the acid at 100℃ to obtain hydrochloric acid decomposition solution; (7) The hydrochloric acid decomposition solution was filtered to remove impurities, concentrated at low temperature, and spray-dried to obtain the animal-derived amino acid protein peptide; The amount of trypsin added in step (3) is 20-22% of the weight of the first fermentation broth; The amount of trypsin added in step (5) is 10-12% of the weight of the sulfuric acid decomposition solution; The hydrochloric acid solution in step (6) has a pH of 4 and is added at 10-12% of the weight of the second fermentation broth. The acid decomposition time is 8-9 hours.
2. The method for preparing animal-derived amino acid protein peptides according to claim 1, characterized in that, The fermentation time in step (2) is 8-10 hours and the fermentation temperature is 45℃; 20%-25% of the weight of the first enzymatic hydrolysate of trypsin is added during the fermentation process.
3. The method for preparing animal-derived amino acid protein peptides according to claim 1, characterized in that, The sulfuric acid solution in step (4) has a pH of 2 and is added in an amount of 20-22% of the weight of the second enzymatic hydrolysate. The acid decomposition time is 10-12 hours.
4. The method for preparing animal-derived amino acid protein peptides according to claim 1, characterized in that, The low-temperature vacuum concentration in step (7) involves filtering the hydrochloric acid decomposition solution to remove impurities and concentrating it to 70% at 60-70°C.