Traditional Chinese medicine composition for preventing and treating lung tumor, preparation method and application thereof

Abstract

The present invention belongs to the field of tumor medicine technology, and in particular to a Chinese medicine composition for preventing and treating lung tumors, and its preparation method and application. The Chinese medicine composition of the present invention comprises the following raw materials in parts by weight: 30 to 42 parts by weight of white chrysanthemum, 35 to 45 parts by weight of ligusticum chuanxiong, 15 to 28 parts by weight of purslane, 12 to 17 parts by weight of salvia miltiorrhiza, 5 to 15 parts by weight of angelica, 16 to 22 parts by weight of parsley, and 10 to 15 parts by weight of sophora japonica. After oral administration of the Chinese medicine composition of the present invention, the separated animal serum has an anti-proliferation inhibitory effect on A549, IR, PC-9, and LLC cells and an inhibitory effect on tumor cell metastasis; significantly improves the proliferation of mouse lung cancer cells; through pharmacological network analysis, it is found that the Chinese medicine composition of the present invention exerts an anti-tumor effect by slowing down cell migration and exerts its anti-non-small cell lung cancer effect by regulating the PI3K-AKT / lipid metabolism pathway.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of tumor medicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating lung tumors and a preparation method and application thereof. BACKGROUND

[0002] Lung cancer is one of the malignant tumors with high morbidity and mortality worldwide, and non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancer. At present, the main treatment methods for lung cancer include surgery, radiotherapy, chemotherapy and targeted therapy, among which chemotherapy is the most common treatment method. However, patients often have drug resistance and varying degrees of side effects after taking the medicine. At the same time, with the continuous deepening of tumor research, a large number of anticancer drugs have been developed by scientific researchers, but most of the drugs still have great toxic side effects and low selectivity, so many candidate drugs are stopped in clinical research. Although chemically synthesized antitumor drugs have a place in the clinic, as people increasingly realize their toxic side effects, it is still an urgent problem to find and develop new antitumor drugs with high efficiency and low toxicity. Traditional Chinese medicine has a long history in the treatment of lung cancer and has unique characteristics, so finding new drugs from traditional Chinese medicine has attracted the attention of many pharmacologists.

[0003] According to traditional Chinese medicine theory, lung tumors belong to the categories of "lung accumulation", "cough", "hemoptysis", "chest pain" and "asthma". Deficiency of vital qi and imbalance of viscera yin and yang are the main basis for suffering from lung cancer. Due to deficiency of vital qi and imbalance of viscera yin and yang, evil toxin attacks the lung, stagnates in the chest, causes lung dysfunction, fails to discharge and descend, obstructs blood flow, and causes accumulation of fluid, which forms phlegm, phlegm and blood coagulate, and finally forms lung masses. Therefore, lung cancer is a disease of deficiency in the whole body and excess in the local part, deficiency of vital qi is the root and excess of evil is the branch. The disease is located in the lung and is closely related to the liver, spleen and kidney. The basic treatment principle is to support the healthy qi and eliminate evil, and treat the branch and the root. In the early stage of the disease, the main problem is excess of evil, if the healthy qi is not deficient, the evil should be eliminated first (including surgical removal of tumor), and the healthy qi should be supported. In the late stage of lung cancer, the main problem is deficiency of vital qi, and the treatment should support the healthy qi and eliminate the evil. Through reasonable tonification, the body state is improved, which helps to improve the anticancer ability and delay the rapid deterioration of the disease.

[0004] With the rapid development of modern Chinese medicine pharmacology, traditional Chinese medicine oncology has been transformed into a mode of treating tumors with Chinese characteristics of traditional Chinese and Western medicine combination, with micro-differentiation, overall prevention and treatment combined with flexible use of modern medical technology, instead of the past macro-differentiation. China has rich traditional Chinese medicine resources. In recent years, researchers have carried out a large number of screening on traditional Chinese medicine monomers or compound traditional Chinese medicine with tumor activity, which shows that anti-tumor traditional Chinese medicine has many mechanisms of action, such as regulating immune function of the body, inhibiting tumor angiogenesis, directly killing tumor cells, inducing tumor cell apoptosis, inducing tumor cell differentiation, reversing multiple drug resistance of cancer cells, regulating cell signal transduction, and inhibiting telomerase activity. Traditional Chinese medicine has many characteristics that western medicine cannot have, such as multiple targets, multiple links, and wide pharmacological effects. The same traditional Chinese medicine often regulates the whole body to improve the body's own disease resistance and achieve the effect of anti-tumor through multiple mechanisms. With the continuous deepening of experimental and clinical research on the anti-tumor effect of compound traditional Chinese medicine, the anti-tumor effect of traditional Chinese medicine is increasingly recognized by the international community. Traditional Chinese medicine combined with small molecule inhibitors has unique advantages in the treatment of lung cancer, which can not only relieve the clinical symptoms of patients, but also reduce the complications and adverse reactions of small molecule inhibitors.

[0005] The present application is based on network pharmacology and serum drug chemistry. By integrating information on drug molecular structure, mechanism of action, molecular pathway, serum metabolites and the like in bioinformatics and systems biology, the therapeutic effect of drugs is comprehensively presented through calculation technology, neural network and the like. The present application uses network pharmacology and serum drug chemistry to construct a component-target-disease interaction network, and combines computer-aided design, animal experiments and cell experiments to further explore the main effective components and specific mechanisms of the traditional Chinese medicine composition in treating NSCLC, thereby providing a direction and a train of thought for future research on traditional Chinese medicine treatment of NSCLC. Therefore, the present application provides a traditional Chinese medicine composition for preventing and treating lung tumors. SUMMARY

[0006] The present application provides a traditional Chinese medicine composition for preventing and treating lung tumors, which can inhibit the proliferation and metastasis of tumor cells, enhance the efficacy of chemotherapeutic drugs, improve the immune function of the body, and has good effects on the recovery of patients after lung tumor surgery. The traditional Chinese medicine composition is made of pure natural traditional Chinese medicinal materials, is rapidly decomposed and absorbed after oral administration, has no digestive tract inhibition and other adverse reactions.

[0007] Another object of the present application is to provide a preparation method of the traditional Chinese medicine composition, which extracts beneficial components from raw materials in multiple ways, so that the beneficial components are fully released and the efficacy of the prescription is significantly improved. The present application also provides an application of the traditional Chinese medicine composition in preparing a medicine for preventing and / or treating lung tumors.

[0008] The technical scheme of the present application is as follows:

[0009] The traditional Chinese medicine composition for preventing and treating lung tumors comprises the following raw materials in parts by weight: white chrysanthemum 30-42 parts by weight, Szechuan lovage 35-45 parts by weight, spadeleaf 15-28 parts by weight, red sage root 12-17 parts by weight, Chinese angelica 5-15 parts by weight, yucca 16-22 parts by weight, and Japanese pagoda tree flower 10-15 parts by weight.

[0010] Preferably, the white chrysanthemum is 35-38 parts by weight, the Szechuan lovage is 38-42 parts by weight, the spadeleaf is 20-26 parts by weight, the red sage root is 12-15 parts by weight, the Chinese angelica is 8-12 parts by weight, the yucca is 18-20 parts by weight, and the Japanese pagoda tree flower is 10-13 parts by weight.

[0011] Further preferably, the white chrysanthemum is 36.8 parts by weight, the Szechuan lovage is 40.5 parts by weight, the spadeleaf is 22.3 parts by weight, the red sage root is 13.5 parts by weight, the Chinese angelica is 10.5 parts by weight, the yucca is 18.4 parts by weight, and the Japanese pagoda tree flower is 11.7 parts by weight.

[0012] The preparation method of the traditional Chinese medicine composition for preventing and treating lung tumors comprises the following steps:

[0013] (1) The raw materials are washed, dried, alcohol-extracted, concentrated under reduced pressure, and then an extract one and a residue one are obtained;

[0014] The alcohol extraction method is as follows: the raw materials are added into an ethanol solution, soaked at 50-60°C for 2-4 days, and the weight ratio of the raw materials to the ethanol solution is 1:(8-10), and the volume concentration of the ethanol solution is 95%-99%.

[0015] (2) The residue one is soaked, decocted, and concentrated in water to obtain an extract two and a residue two;

[0016] (3) The residue two is again soaked, decocted, and concentrated in water to obtain an extract three;

[0017] In steps (2) and (3), the mass ratio of the residue one or the residue two to water is 1:(5-10); the soaking time is 10-40 min, the decocting time is 40-120 min, and the concentration ratio is 10-15 times;

[0018] (4) The extract one, the extract two, and the extract three are combined to obtain the traditional Chinese medicine composition, which is bottled, sealed, and stored at 4°C.

[0019] The application of the traditional Chinese medicine composition in the preparation of a medicine for preventing and / or treating lung tumors.

[0020] The application of the traditional Chinese medicine composition in the preparation of a medicine for inhibiting the proliferation of non-small cell lung cancer cells, wherein the traditional Chinese medicine composition can inhibit the proliferation of A549 (human non-small cell lung cancer cells), IR (human non-small cell lung cancer cell strain with EGFR L858R / T790MA549 (human lung cancer cell line with EGFR mutation), PC-9 (human lung cancer cell line with EGFR mutation) proliferation. del19 A549 (human lung cancer cell line with EGFR mutation), PC-9 (human lung cancer cell line with EGFR mutation) proliferation.

[0021] The traditional Chinese medicine composition can inhibit the proliferation of LLC (mouse non-small cell lung cancer cells).

[0022] A medicine for inhibiting lung tumors, comprising a therapeutically effective amount of the traditional Chinese medicine composition of the present application and a pharmaceutically acceptable excipient, which can be prepared into tablets, capsules, granules, pills, extracts or oral liquids.

[0023] The present application carries out the anti-tumor activity research of the traditional Chinese medicine composition, finds that the traditional Chinese medicine composition has a significant therapeutic effect on non-small cell lung cancer cells, and the traditional Chinese medicine composition has no inhibitory effect on normal cells, low toxicity and good safety, and the specific research includes:

[0024] (1) The efficacy test example 1 research finds that after the traditional Chinese medicine composition of the present application is administered by gavage, the separated animal serum has an anti-proliferation inhibitory effect on A549, IR, PC-9 and LLC cells and an inhibitory effect on tumor cell metastasis;

[0025] (2) The efficacy test example 2 research finds that the traditional Chinese medicine composition of the present application can significantly improve the proliferation of lung cancer cells in mice by gavage administration;

[0026] (3) Through pharmacological network analysis, it is found that the traditional Chinese medicine composition of the present application plays an anti-tumor role by slowing down cell migration and plays an anti-non-small cell lung cancer role by regulating the PI3K-AKT / lipid metabolism pathway.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] 1. The traditional Chinese medicine composition of the present application collects 7 kinds of raw materials, taking white chrysanthemum and Szechuan lovage root as monarch drugs, white chrysanthemum plays a role of clearing heat and detoxifying, chrysanthemum is cold in nature, disperses wind-heat, eliminates swelling and detoxifies, plays an anticancer and swelling-eliminating role, Szechuan lovage root has the effects of removing blood stasis, relieving pain and regulating qi and relieving depression; the traditional Chinese medicine composition can inhibit the proliferation and metastasis of tumor cells, enhance the curative effect of chemotherapeutic drugs and improve the immune function of the body; it also has a good effect on the recovery of the patient's body after lung tumor surgery, effectively prevents the recurrence of lung tumors after surgery, effectively prolongs the survival time of the patient and improves the quality of life of the patient.

[0029] 2. The traditional Chinese medicine composition of the present application uses pure natural Chinese medicinal materials, the raw materials are easy to obtain, has broad applicability, is safe and has no toxic side effects, is rapidly decomposed and absorbed after oral administration, has no digestive tract inhibition and other adverse reactions.

[0030] 3. The preparation method of the traditional Chinese medicine composition is simple, the preparation cost is low, and the beneficial components in the raw materials are extracted in multiple ways, so that the beneficial components are fully released, and the efficacy of the prescription is significantly improved. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 , the inhibitory activity of the traditional Chinese medicine composition on A549 and LLC cells, A: the inhibition rate and IC 50 of the traditional Chinese medicine composition on A549 cells; B: the inhibition rate and IC 50 of the traditional Chinese medicine composition on LLC cells;

[0032] Figure 2 , the effect of drug-containing serum on the migration of A549 cells;

[0033] Figure 3 , the effect of the traditional Chinese medicine composition on the C57 mouse tumor model, A: the effect of the traditional Chinese medicine composition on the body weight of C57 mice; B: the effect of the traditional Chinese medicine composition on the tumor volume of C57 mice; C: the effect of the traditional Chinese medicine composition on the tumor of C57 mice;

[0034] Figure 4 , the effect diagram of the traditional Chinese medicine composition on the C57 mouse tumor model;

[0035] Figure 5 , the intersection diagram of drug target points and disease target points;

[0036] Figure 6 , the drug component-target point relationship network diagram;

[0037] Figure 7 , the core target point schematic diagram;

[0038] Figure 8 , the BP, CC, and MF result schematic diagram;

[0039] Figure 9 , the KEGG result schematic diagram. DETAILED DESCRIPTION

[0040] In order to verify the anti-tumor effect of the traditional Chinese medicine composition, the inventors carried out pharmacodynamic experiment. It should be noted that the following pharmacodynamic experiment selects the most representative formula and the prepared medicine obtained by the preparation method of the present application; the inventors also carried out pharmacodynamic experiments on other formulas and the prepared medicine obtained by the preparation method of the present application, and the experimental results show that the other formulas and the prepared medicine obtained by the preparation method have the same or similar efficacy, but due to the limited space, they are not listed here; in addition, the following pharmacodynamic experiment takes in vitro experiment and part of the representative animal model as an example to verify the efficacy of the present application, and the following experimental study is carried out on the basis of acute toxicity experiment and long-term toxicity experiment to prove the safety of the medicine.

[0041] Example 1

[0042] The traditional Chinese medicine composition for preventing and treating lung tumor comprises the following raw materials by weight:

[0043] 36.8 parts by weight of white chrysanthemum, 40.5 parts by weight of chuanxiong, 22.3 parts by weight of purslane, 13.5 parts by weight of danshen, 10.5 parts by weight of angelica, 18.4 parts by weight of yunnan bletilla, and 11.7 parts by weight of sophora flower;

[0044] The preparation method is as follows:

[0045] (1) The raw materials are washed and dried, and then subjected to alcohol extraction, and the extract one and the residue one are obtained after concentration by reduced pressure evaporation;

[0046] The alcohol extraction method is as follows: the raw materials are added into ethanol solution, and soaked at 60℃ for 2d, the weight ratio of the raw materials to the ethanol solution is 1:8, and the volume concentration of the ethanol solution is 95%.

[0047] (2) The residue one is soaked, decocted and concentrated in water to obtain the extract two and the residue two;

[0048] (3) The residue two is again soaked, decocted and concentrated in water to obtain the extract three;

[0049] In steps (2) and (3), the mass ratio of the residue one or the residue two to water is 1:5, the soaking time is 30min, the decocting time is 60min, and the concentration ratio is 10 times;

[0050] (4) The extract one, the extract two and the extract three are combined to obtain the traditional Chinese medicine composition, which is bottled, sealed and stored at 4℃.

[0051] Example 2

[0052] The traditional Chinese medicine composition for preventing and treating lung tumor comprises the following raw materials by weight:

[0053] Baij 30 parts by weight, Chuanqiong 35 parts by weight, Maqian 15 parts by weight, Danshen 12 parts by weight, Dangui 5 parts by weight, Chonglou 16 parts by weight, Huaihua 10 parts by weight;

[0054] The preparation method is referred to Example 1.

[0055] Example 3

[0056] The traditional Chinese medicine composition for preventing and treating lung tumor according to the present application comprises the following raw materials in parts by weight:

[0057] Baij 42 parts by weight, Chuanqiong 45 parts by weight, Maqian 28 parts by weight, Danshen 17 parts by weight, Dangui 15 parts by weight, Chonglou 22 parts by weight, Huaihua 15 parts by weight;

[0058] The preparation method is referred to Example 1.

[0059] The traditional Chinese medicine composition according to the present application is subjected to anti-tumor test as follows:

[0060] 1. Pharmacodynamic test Example 1 in vitro anti-tumor experiment

[0061] 1.1 Experimental method

[0062] (1) Preparation of drug-containing serum

[0063] Take 48 mice, with a body weight of 17-20 g, all male; divide them into two groups, a normal control group and a drug administration group; the normal control group: normal saline, the drug administration group: the extract in Example 1, 0.97 g / kg of crude drug;

[0064] Administer intragastrically twice a day for 3 consecutive days, 1 h after the last administration or normal saline, anesthetize with sodium pentobarbital, take blood from the inferior vena cava, centrifuge to separate serum, obtain serum at the 1 h time point, inactivate at 56℃ for 30 min, and store in a refrigerator at -20℃ for standby.

[0065] (2) Cell culture and resuscitation

[0066] Before resuscitation, preheat the water bath to 37℃ and turn on the ultraviolet lamp of the clean bench for irradiation for more than 30 min, then quickly take out the pre-experimental cell cryopreservation tube from the liquid nitrogen tank and immediately put it into the 37℃ water bath, shake it to melt as soon as possible. Then transfer the cell cryopreservation tube to the centrifuge and centrifuge at 1000 rpm for 5 min. After the end, discard the supernatant in the clean bench, resuspend the cells with 1 mL of corresponding culture medium containing 10% FBS by blowing gently, and transfer them to a new culture bottle, add an appropriate amount of corresponding culture medium containing 10% FBS, shake gently to make the cells evenly distributed, then observe the number and morphology of the cells under a microscope. Place the culture bottle in a 37℃, 5% CO2 incubator for culture.

[0067] (3) Determination of the effect of drug-containing serum on cell lines

[0068] Different tumor cells were inoculated in 96-well plates, 100 μL of cell suspension was added to each well, and the cell density was 5 x 10 3 cells per well for A549, LLC, PC-9, and IR cells, respectively. The cells were incubated in a cell culture incubator for 24 h. The next day, the drug-containing serum was treated at concentrations of 1%, 2%, 5%, 10%, and 20%, with 4 replicate wells for each concentration. After 72 h, 10 μL of MTT detection reagent was added to each well, and the cells were incubated in a cell culture incubator for another 4 h. The culture medium was aspirated, 150 μL of DMSO was added to each well, and the mixture was shaken for 1 min. Finally, the absorbance at 490 nm (OD value) was measured using a microplate reader. The final cell growth inhibition rate was calculated using the formula: cell growth inhibition rate (%) = {1-[(OD experimental group-OD blank group) / (OD control group-OD blank group)]} x 100%.

[0069] (4) Effect of drug-containing serum on cell migration

[0070] A549 cells in the logarithmic growth phase were inoculated in 6-well plates at a density of 5 x 10 5 cells per well, and incubated for 24 h. The cells were then scratched with a white gun head and washed with PBS 3 times. Different concentrations of drug-containing serum were added to interfere with the cells. At 0 h and 24 h, inverted microscopy (100x) was used to take photographs. The cell migration rate was calculated using Image Analysis and Image J software, and the results were analyzed using GraphPad prism 7.04 software.

[0071] 1.2 Results

[0072] The results of the cell experiment showed that the cell activity gradually decreased after the A549 and LLC cells were treated with the traditional Chinese medicine compound concentrate detected by the MTT method. The IC 50 values were 1.077 mg / mL and 1.317 mg / mL, respectively. See Figure 1 A and B in the middle.

[0073] The drug administration group was administered by gavage, and the drug-containing serum was obtained. The four lung cancer cell lines A549 (human non-small cell lung cancer cell line), IR (non-small cell lung cancer cell line with EGFR L858R / T790M mutation), PC-9 (lung cancer cell line with EGFR del19 mutation), and LLC (murine non-small cell lung cancer cell line) were all inhibited. The drug-containing serum was treated at concentrations of 1%, 2%, 5%, 10%, and 20%, and the inhibition rates of A549, IR, PC-9, and LLC cells were as shown in Table 1.

[0074] Table 1 Inhibition rate of the serum containing the traditional Chinese medicine composition in the application on cell strains

[0075] Cell strain name Inhibition rate of 5% drug-containing serum administration group on tumor cells Inhibition rate of 10% drug-containing serum administration group on tumor cells A549 39.79% 63.75% IR 22.9% 40.33% PC-9 26.17% 45.94% LLC 37.01% 68.07%

[0076] It is illustrated by Table 1 that after the A549, IR, PC-9 and LLC cells are treated by the serum containing the traditional Chinese medicine composition in different concentrations, the inhibition effect similar to that of the traditional Chinese medicine compound concentrated solution is shown, and the inhibition rates of the A549, IR, PC-9 and LLC cells are 39.79%, 22.9%, 26.17% and 37.01% respectively under the 5% serum containing the traditional Chinese medicine composition; and the inhibition rates of the A549, IR, PC-9 and LLC cells are 63.75%, 40.33%, 45.94% and 68.07% respectively under the 10% serum containing the traditional Chinese medicine composition.

[0077] The living cells generally have a certain migration ability, and the migration abilities of different cells are different. The migration ability of most tumor cells is strong, and thus the cell migration ability is also an important index for reflecting the metastasis of tumor cells in vivo. In the application, the A549 cells in the logarithmic growth phase are plated in a 6-well plate, 5x10 5 Figure 2 The results are analyzed by the GraphPad prism 7.04 software. As shown in the results, with the extension of time, the scratch of the cells starts to heal gradually inward, compared with the blank group, the high and medium concentrations of the traditional Chinese medicine concentrated solution can inhibit the metastasis of the A549 cells to a certain extent.

[0078] 2. Pharmacodynamic test example 2 Inhibition effect of the traditional Chinese medicine composition in the application on the lung cancer model of mice

[0079] 2.1 Establishment of non-small cell lung cancer model of mice

[0080] The experimental animals are 25 C57 mice, female, 4-6 weeks old, and the feeding temperature is 20-25℃, and the relative humidity is 60%. These animals are irradiated by a light cycle of light and darkness for 12 hours. The animal drinking water, cage is sterilized by an autoclave, and the materials and bedding are irradiated, and the animals are free to drink water, and the auxiliary materials are changed once a day. After the animals are adaptively fed for 7 days, the model is established. The treatment of the animals is carried out in accordance with the ethical requirements of the experimental animal management committee.

[0081] ​Take DMEM medium 45 mL, fetal bovine serum 5 mL, penicillin-streptomycin double antibody 500 nl, mix on the clean bench, shake well, and then prepare 50 mL complete culture medium. Discard the old cell culture medium, absorb 3 mL PBS to gently moisten the cells, discard the PBS, add 1 mL trypsin, digest for about 1 min, add 1 mL complete medium along the bottle to stop the digestion, and gently blow the cells off. Centrifuge to prepare a cell suspension, and count with a hemocytometer. After counting, adjust the cell suspension concentration to 2×10 7 mL / L of LLC cell density, and gently blow the cell suspension. Randomly select 20 mice for modeling, and subcutaneously inject the cell suspension 0.1 mL into each mouse under the right hip, that is, inoculate 2×10 6 mice with 2×10

[0082] 2.2 In vivo anti-non-small cell lung cancer activity of the traditional Chinese medicine composition

[0083] After tumor formation, measure the tumor diameter every day, weigh the mice, and when the tumor diameter reaches 50 mm 3 , randomly divide the average tumor into 2 groups. Calculate the dosing amount according to the mouse weight, and perform gavage. The control group is given an equal amount of normal saline every day, and the administration group is given the traditional Chinese medicine composition prepared in Example 1 for gavage 0.2 mL / time, 2 times / d, for continuous administration for 21 d. During the treatment period, the mice are raised routinely, and the tumor size of the mice is observed and photographed every 3 days before and after administration. On the 21st day of treatment, the mice are fasted and deprived of water, and after the treatment, the mice are anesthetized with 0.3% sodium pentobarbital solution, the tumor tissue is removed and photographed. As shown in Figure 3 Fig. A, it is found through the research of the administration group, the blank group, and the control group that the mouse weight basically remains unchanged during the experiment, indicating that the traditional Chinese medicine compound does not affect the overall weight of the animals during the anti-tumor process. As shown in Figure 3 Fig. B, it is found through the research of the administration group, the blank group, and the control group that compared with the blank group, the tumor volume of the traditional Chinese medicine administration group is significantly reduced. As shown in Figure 3 Fig. C and Figure 4 , it is found through the research of the administration group, the blank group, and the control group that the tumor volume of the administration group is significantly reduced (P<0.05) under the intervention of the traditional Chinese medicine during the experiment, and is positively correlated with the dose. The traditional Chinese medicine composition of the present application shows an obvious tumor inhibition effect.

[0084] 3. Network pharmacology analysis and processing

[0085] 3.1 Gene annotation of drug targets

[0086] Drug targets were collected using the TCMSP database and the SwissTargetPrediction platform. The active ingredients and targets were screened and de-duplicated using a script written in Perl computer program language, and the names and numbers of drug targets were displayed. On this basis, the drug ingredient name was converted into a gene tag through the Uniprot database, and a total of 534 drug targets were obtained.

[0087] 3.2 Screening of disease-related genes

[0088] The disease targets were searched using the GeneCard database (https: / / www.genecards.org / ), OMIM database (https: / / www.omim.org / ), etc., with the keyword: non-small cell lung cancer, and the data were downloaded, sorted and summarized. A total of 5814 disease target genes were obtained from the Genecard database.

[0089] 3.3 Screening of drug-disease targets

[0090] The label information was separated from the drug target file for later use, and the disease corresponding label information was separated from the GeneCard data for later use; the data were processed using the R language VennDiagram package and a Venn diagram was made (Fig. 3) Figure 5 ), and the drug-disease corresponding data were obtained. Through screening of drug targets, 347 disease and drug common targets were obtained (Fig. 4) Figure 6 ).

[0091] As Figure 7 , the disease-drug interaction proteins were imported into the String PPi protein interaction network (PPI) analysis database, the human database was selected, the score (confidence) was set to >0.9 after adjustment, the free nodes were hidden, and the PPI protein interaction network node graph was generated. According to the existing data, 18448 protein interaction connections were generated from the PPI database. Subsequently, the R language was used to enrich the connection results and make a visual column chart, in which the top five targets with the strongest force were TP53, JUN, IL6, TNF and AKT1.

[0092] The disease drug target data were transformed using the R language BiocManager package, and the gene ID was displayed to facilitate later data enrichment analysis; the corresponding package was installed in R language, the corresponding parameters were set, the GO gene function was enriched, and a column chart (Fig. 6) Figure 8 ) and a scatter plot (Fig. 7) Figure 9The GO enrichment results are screened by using a Perl computer program language to obtain the functions of the corresponding genes. GO function enrichment analysis in R language obtains 3715 GO entries (P<0.05). Analysis of the mechanism finds that the mechanism of the Chinese medicine composition in playing the role of resisting non-small cell lung cancer may be related to PI3K-AKT, lipid metabolism and the like.

[0093] The above is only a preferred embodiment of the present application, and does not limit the present application in any form; any person skilled in the art can easily implement the present application according to the drawings and the above description; however, any equivalent changes, modifications and evolutions made by those skilled in the art within the scope of the technical solutions of the present application, using the above disclosed technical content, are equivalent embodiments of the present application; at the same time, any equivalent changes, modifications and evolutions made according to the essence of the present application to the above embodiments are still within the protection scope of the technical solutions of the present application.

Claims

1. A Chinese medicine composition for preventing and adjuvant treatment of lung tumors, characterized in that: The invention is composed of the following raw materials in parts by weight: 30-42 parts by weight of feverfew, 35-45 parts by weight of chuanxiong, 15-28 parts by weight of purslane, 12-17 parts by weight of salvia miltiorrhiza, 5-15 parts by weight of angelica, 16-22 parts by weight of paridis and 10-15 parts by weight of sophora japonica flower.

2. The Chinese medicine composition according to claim 1, characterized in that The invention is composed of the following raw materials in parts by weight: 36.8 parts by weight of feverfew, 40.5 parts by weight of ligusticum chuanxiong, 22.3 parts by weight of purslane, 13.5 parts by weight of salvia miltiorrhiza, 10.5 parts by weight of angelica sinensis, 18.4 parts by weight of paridis and 11.7 parts by weight of sophora japonica flower.

3. A method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are involved: (1) The raw material is washed and dried, subjected to alcohol extraction, and concentrated by evaporation under reduced pressure to obtain an extract and a medicinal residue; (2) soaking the medicinal residue 1 in water, boiling, and concentrating to obtain the extract 2 and the medicinal residue 2; (3) soaking the second medicinal residue in water again, boiling, and concentrating to obtain the third extract; (4) Combining extract 1, extract 2 and extract 3 to obtain the Chinese medicine composition.

4. The method for preparing the Chinese medicine composition according to claim 3, wherein The alcohol extraction method is as follows: adding the raw material into an ethanol solution and soaking it at 50-60° C. for 2-4 days, wherein the weight ratio of the raw material to the ethanol solution is 1:(8-10), and the volume concentration of the ethanol solution is 95%-99%.

5. The method for preparing the Chinese medicine composition according to claim 3, wherein The mass ratio of the medicinal residue one or the medicinal residue two to water is 1:(5-10).

6. The method for preparing the Chinese medicine composition according to claim 3, wherein: In steps (2) and (3), the soaking time is 10 to 40 minutes, and the decoction time is 40 to 120 minutes.

7. Use of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of a drug for preventing and / or treating lung tumors.

8. Use of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of a drug for inhibiting the proliferation of non-small cell lung cancer cells.

9. A drug for inhibiting lung tumors, characterized in that: The medicine comprises a therapeutically effective amount of the traditional Chinese medicine composition according to claim 1 or 2, and pharmaceutically acceptable excipients.

10. The drug according to claim 9, characterized in that The medicine is prepared into tablets, capsules, granules, pills, extracts or oral liquids.

Images