A whitening plant polysaccharide composition

Abstract

The present invention relates to a whitening plant polysaccharide composition, and belongs to the technical field of plant polysaccharide compositions. The preparation method of the plant polysaccharide composition comprises the following steps: 1) adding a bacterial agent to a mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment; 2) homogenizing egg yolk lecithin, cholesterol, polyglycerol-10, butanediol, dendrobium extract, glycyrrhizic flavonoids, glycyrrhizic acid, deionized water and the mixed fermentation product to obtain a plant polysaccharide composition. The present invention adopts a combination of dendrobium extract, glycyrrhizic flavonoids, glycyrrhizic acid and a mixed fermentation product, and reduces the synthesis of dopaquinone and melanin by inhibiting tyrosinase activity, different anti-inflammatory mechanisms and antioxidant mechanisms, and can inhibit the occurrence of oxidative stress, thereby reducing the production of melanin.

Description

Technical Field

[0001] The invention belongs to the technical field of plant polysaccharide compositions and relates to a whitening plant polysaccharide composition. Background Art

[0002] Cosmetics with whitening effects have always been popular among consumers and are also an important research direction for researchers in the cosmetics field. The degree of skin color is closely related to the melanin content in skin cells. The production of melanin is closely related to tyrosinase, which is specifically manifested in: tyrosinase catalyzes tyrosine to form dopa, and then continues to catalyze the formation of dopaquinone. Dopaquinone is then rearranged, polymerized, and combined with structural proteins in melanosomes to form melanin. Tyrosinase is an important rate-limiting enzyme in the production of melanin. Inhibiting tyrosinase activity can reduce the production of melanin, which is beneficial to whitening.

[0003] In addition, human skin also stores a large amount of water, fat, protein, sugar and vitamins, which together participate in the metabolic process of the entire body. When the body's metabolism is affected, such as: external environment, unhealthy diet, mental stress and other influences, it will lead to skin problems such as dry skin, dullness and aging. Summary of the invention

[0004] The object of the present invention is to provide a whitening plant polysaccharide composition. By adopting a combination of dendrobium extract, licorice flavonoids, glycyrrhizic acid and a mixed fermentation product, the synthesis of dopaquinone and melanin is reduced by inhibiting tyrosinase activity, different anti-inflammatory mechanisms and antioxidant mechanisms, and the occurrence of oxidative stress can be inhibited, thereby reducing the production of melanin. The whitening plant polysaccharide composition prepared by the scheme of the present invention has a moisturizing effect and is excellent in protecting and repairing the skin from oxidative damage.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A whitening plant polysaccharide composition, wherein the preparation method of the plant polysaccharide composition comprises the following steps:

[0007] 1) adding bacterial agent to the mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment;

[0008] 2) homogenizing egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin flavonoids, glycyrrhizic acid, deionized water and mixed fermentation product to obtain a plant polysaccharide composition.

[0009] As a preferred technical solution of the present invention, in step 1), ultraviolet sterilization is also included before the mixed extract is fermented; the mixed extract includes plant extract, concentrated extract of dendrobium residue, glucose and ammonium sulfate; the bacterial agent is brewer's yeast; the mass ratio of glucose, ammonium sulfate, concentrated extract of dendrobium residue and plant extract is 0.1-0.5:0.1-0.3:18-20:70-75; the plant extract is a mixture of Eryngium marinum extract, turmeric extract and rose callus extract in a mass ratio of 2:0.4-0.8:0.8-1.2; the number of bacterial agents inoculated in the mixed extract is 10 5 -10 6 CFU / mL, the fermentation condition is fermentation at 25-35°C for 30-48h; the ultrasonic treatment is at 80-140w for 10-20min.

[0010] As a preferred technical solution of the present invention, in step 2), the mass ratio of the egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizic acid, deionized water and mixed fermentation product is 1: 0.05-0.125: 0.3-0.8: 1.0-4.5: 2.0-5.6: 0.15-0.5: 0.25-0.50: 15-25: 1-4.

[0011] In the scheme of the present invention, the active ingredients in the Eryngium littoralis extract reduce the synthesis of melanin by inhibiting the tyrosinase activity of melanin production or by anti-oxidation; the active ingredients in the concentrated extract of Dendrobium candidum reduce the synthesis of melanin by inhibiting the tyrosinase activity of melanin production, anti-inflammatory effect and antioxidant effect. In addition, the concentrated extract of Dendrobium candidum has good moisturizing effect, protection and repair effect against oxidative damage on human skin; the active ingredients in the turmeric extract can reduce the synthesis of melanin by inhibiting the tyrosinase activity of melanin production, anti-inflammatory effect and antioxidant effect; the active ingredients in the rose callus extract reduce the synthesis of melanin by inhibiting the tyrosinase activity of melanin production, anti-inflammatory effect and antioxidant effect; glycyrrhizic flavonoids reduce the synthesis of melanin by inhibiting the tyrosinase activity of melanin production, anti-inflammatory effect and antioxidant effect; glycyrrhizic acid reduces the synthesis of melanin by inhibiting the tyrosinase activity of melanin production, anti-inflammatory effect and antioxidant effect.

[0012] The invention discloses a method for preparing the dendrobium extract, comprising the following steps:

[0013] (1) crushing and sieving the Dendrobium officinale, adding deionized water, extracting and treating the mixture multiple times, centrifuging the mixture, and combining the supernatants to obtain a Dendrobium officinale supernatant;

[0014] (2) concentrating the dendrobium supernatant under reduced pressure to obtain a dendrobium concentrated supernatant;

[0015] (3) adding ethanol to the concentrated supernatant of Dendrobium officinale, refrigerating at low temperature, and discarding the supernatant after centrifugation to obtain the Dendrobium officinale extract.

[0016] As a preferred technical solution of the present invention, in step (1), the sieving is through a 40-mesh sieve, the dosage ratio of the Dendrobium officinale and deionized water is 1 g: 25-28 mL; the multiple extraction treatment is extraction at 70-80° C. for 2-3 times; the centrifugation condition is centrifugation at a speed of 5000-6000 r / min for 8-12 min;

[0017] As a preferred technical solution of the present invention, in step (1), it also includes the step of combining the waste residues after centrifugation to obtain Dendrobium waste residues.

[0018] As a preferred technical solution of the present invention, in step (2), the reduced pressure concentration condition is to concentrate at 55-60°C.

[0019] As a preferred technical solution of the present invention, in step (3), the volume ratio of the concentrated supernatant of Dendrobium officinale to ethanol is 1:4.0-4.5; the ethanol is anhydrous ethanol, and the low-temperature refrigeration is refrigeration at 4°C; the centrifugation condition is at 7500-8000r / min for 8-12min;

[0020] The invention discloses a method for preparing the concentrated extract of dendrobium residue, comprising the following steps:

[0021] S1. After adding deionized water to the waste residue of Dendrobium, ultrasonic treatment is performed, cellulase and ligninase are added, and after enzymolysis, heating is performed to obtain a Dendrobium residue extract;

[0022] S2, concentrating the dendrobium residue extract under reduced pressure to obtain a dendrobium residue concentrated extract.

[0023] As a preferred technical solution of the present invention, in step S1, the dosage ratio of the Dendrobium waste residue and deionized water is 1kg:8-10L, the ultrasonic treatment is at 100-120w, and the ultrasonic treatment is 15-25min; the mass ratio of the Dendrobium waste residue, cellulase and ligninase is 10:0.1-0.2:0.06-0.08, and the enzymatic hydrolysis is 10-15min; the heating condition is heating to 85-95℃.

[0024] As a preferred technical solution of the present invention, in step S2, the reduced pressure concentration condition is to concentrate at 60-80°C, and the dosage ratio of the concentrated extract of dendrobium residue to dendrobium officinale is 3-5L:1kg.

[0025] Beneficial effects of the present invention:

[0026] The scheme of the present invention adopts a combination of dendrobium extract, licorice flavonoids, glycyrrhizic acid and mixed fermentation products to inhibit tyrosinase activity, different anti-inflammatory mechanisms and antioxidant mechanisms, reduce the synthesis of dopaquinone and melanin, inhibit the occurrence of oxidative stress, and thus reduce the production of melanin. DETAILED DESCRIPTION

[0027] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0028] Example 1

[0029] A whitening plant polysaccharide composition, wherein the preparation method of the plant polysaccharide composition comprises the following steps:

[0030] 1) adding a bacterial agent to a mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment; wherein, the mixed extract is further sterilized by ultraviolet light before fermentation; the mixed extract comprises a plant extract, a concentrated extract of dendrobium residue, glucose and ammonium sulfate; the bacterial agent is saccharomyces cerevisiae; the mass ratio of the glucose, ammonium sulfate, the concentrated extract of dendrobium residue and the plant extract is 0.1:0.1:18:70; the plant extract is a mixture of a marine eryngium extract, a turmeric extract and a rose callus extract in a mass ratio of 2:0.4:0.8; the number of bacterial agents inoculated in the mixed extract is 10 5 CFU / mL, the fermentation condition is fermentation at 25°C for 30h; the ultrasonic treatment is at 80w for 20min;

[0031] 2) Homogenize egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product to obtain a plant polysaccharide composition; wherein the mass ratio of the egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product is 1:0.05:0.3:1.0:2.0:0.15:0.25:15:1.

[0032] The preparation method of the dendrobium extract comprises the following steps:

[0033] (1) crushing and sieving the Dendrobium officinale, adding deionized water, performing multiple extraction treatments, centrifuging, combining the supernatant and the waste residue, and obtaining a Dendrobium officinale supernatant and a Dendrobium officinale waste residue; wherein the sieving is to pass through a 40-mesh sieve, the amount ratio of the Dendrobium officinale and the deionized water is 1 g: 25 mL; the multiple extraction treatment is to extract twice at 70° C.; the centrifugation condition is to centrifuge at a speed of 5000 r / min for 8 min;

[0034] (2) concentrating the supernatant of Dendrobium officinale under reduced pressure to obtain a concentrated supernatant of Dendrobium officinale; wherein the concentrating under reduced pressure is performed at 55° C.;

[0035] (3) adding ethanol to the concentrated supernatant of Dendrobium officinale, refrigerating at low temperature, and discarding the supernatant after centrifugation to obtain a Dendrobium officinale extract; wherein the volume ratio of the concentrated supernatant of Dendrobium officinale to ethanol is 1:4.0; the ethanol is anhydrous ethanol, and the low temperature refrigeration is refrigeration at 4°C; the centrifugation condition is at 7500 r / min for 8 minutes;

[0036] The preparation method of the concentrated extract of dendrobium residue comprises the following steps:

[0037] S1. After adding deionized water to the waste residue of Dendrobium, ultrasonic treatment is performed, cellulase and ligninase are added, and after enzymolysis, heating is performed to obtain a Dendrobium residue extract; wherein the dosage ratio of the waste residue of Dendrobium to deionized water is 1kg:8L, the ultrasonic treatment is performed at 100w for 15min; the mass ratio of the waste residue of Dendrobium, cellulase and ligninase is 10:0.1:0.06, and the enzymolysis is performed for 10min; the heating condition is heating to 85°C;

[0038] S2. Concentrating the dendrobium residue extract under reduced pressure to obtain a dendrobium residue concentrated extract; wherein the reduced pressure concentration condition is to concentrate at 60° C., and the dosage ratio of the dendrobium residue concentrated extract to the dendrobium officinale is 3L:1kg.

[0039] Example 2

[0040] A whitening plant polysaccharide composition, the preparation method of the plant polysaccharide composition comprising the following steps:

[0041] 1) adding a bacterial agent to a mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment; wherein, the mixed extract is further sterilized by ultraviolet light before fermentation; the mixed extract comprises a plant extract, a concentrated extract of dendrobium residue, glucose and ammonium sulfate; the bacterial agent is saccharomyces cerevisiae; the mass ratio of glucose, ammonium sulfate, concentrated extract of dendrobium residue and plant extract is 0.3:0.2:19:72.5; the plant extract is a mixture of Eryngium marinum extract, turmeric extract and rose callus extract in a mass ratio of 2:0.6:1.0; the number of bacterial agents inoculated in the mixed extract is 5×10 5 CFU / mL, the fermentation condition is fermentation at 28°C for 40h; the ultrasonic treatment is at 100w for 15min;

[0042] 2) homogenizing egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product to obtain a plant polysaccharide composition; wherein the mass ratio of the egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product is 1:0.08:0.5:2.3:3.8:0.32:0.40:18:2.5.

[0043] The preparation method of the dendrobium extract comprises the following steps:

[0044] (1) crushing and sieving the Dendrobium officinale, adding deionized water, extracting and treating for multiple times, centrifuging, combining the supernatant and the waste residue, and obtaining a Dendrobium officinale supernatant and a Dendrobium officinale waste residue; wherein the sieving is to pass through a 40-mesh sieve, the amount ratio of the Dendrobium officinale and the deionized water is 1 g: 26.5 mL; the multiple extraction treatment is to extract twice at 75° C.; the centrifugation condition is to centrifuge at a speed of 5500 r / min for 10 min;

[0045] (2) concentrating the supernatant of Dendrobium officinale under reduced pressure to obtain a concentrated supernatant of Dendrobium officinale; wherein the concentrating under reduced pressure is performed at 58° C.;

[0046] (3) adding ethanol to the concentrated supernatant of Dendrobium officinale, refrigerating at low temperature, and discarding the supernatant after centrifugation to obtain a Dendrobium officinale extract; wherein the volume ratio of the concentrated supernatant of Dendrobium officinale to ethanol is 1:4.2; the ethanol is anhydrous ethanol, the low temperature refrigeration is refrigeration at 4°C; the centrifugation condition is at 7800 r / min for 10 min;

[0047] The preparation method of the concentrated extract of dendrobium residue comprises the following steps:

[0048] S1. After adding deionized water to the waste residue of Dendrobium, ultrasonic treatment is performed, cellulase and ligninase are added, and after enzymolysis, heating is performed to obtain a Dendrobium residue extract; wherein the dosage ratio of the waste residue of Dendrobium to deionized water is 1kg:9L, the ultrasonic treatment is performed at 110w for 20min; the mass ratio of the waste residue of Dendrobium, cellulase and ligninase is 10:0.15:0.07, and the enzymolysis is performed for 12.5min; the heating condition is heating to 90°C;

[0049] S2. Concentrating the dendrobium residue extract under reduced pressure to obtain a dendrobium residue concentrated extract; wherein the reduced pressure concentration condition is to concentrate at 70° C., and the dosage ratio of the dendrobium residue concentrated extract to the dendrobium officinale is 4 L:1 kg.

[0050] Example 3

[0051] A whitening plant polysaccharide composition, the preparation method of the plant polysaccharide composition comprising the following steps:

[0052] 1) adding a bacterial agent to a mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment; wherein, the mixed extract is further sterilized by ultraviolet light before fermentation; the mixed extract comprises a plant extract, a concentrated extract of dendrobium residue, glucose and ammonium sulfate; the bacterial agent is saccharomyces cerevisiae; the mass ratio of glucose, ammonium sulfate, concentrated extract of dendrobium residue and plant extract is 0.5:0.3:20:75; the plant extract is prepared by mixing a marine eryngium extract, a turmeric extract and a rose callus extract in a mass ratio of 2:0.8:1.2; the number of bacterial agents inoculated in the mixed extract is 10 6 CFU / mL, the fermentation condition is fermentation at 35°C for 48h; the ultrasonic treatment is at 140w for 10min;

[0053] 2) homogenizing egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product to obtain a plant polysaccharide composition; wherein the mass ratio of the egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product is 1:0.125:0.8:4.5:5.6:0.5:0.50:25:4.

[0054] The preparation method of the dendrobium extract comprises the following steps:

[0055] (1) crushing and sieving the Dendrobium officinale, adding deionized water, extracting for multiple times, centrifuging, combining the supernatant and the waste residue, and obtaining a Dendrobium officinale supernatant and a Dendrobium officinale waste residue; wherein the sieving is through a 40-mesh sieve, the amount ratio of the Dendrobium officinale and the deionized water is 1 g: 28 mL; the multiple extraction treatment is extraction for 3 times at 80° C.; the centrifugation condition is centrifugation at a speed of 6000 r / min for 12 min;

[0056] (2) concentrating the supernatant of Dendrobium officinale under reduced pressure to obtain a concentrated supernatant of Dendrobium officinale; wherein the concentrating under reduced pressure is performed at 60° C.;

[0057] (3) adding ethanol to the concentrated supernatant of Dendrobium officinale, refrigerating at low temperature, and discarding the supernatant after centrifugation to obtain a Dendrobium officinale extract; wherein the volume ratio of the concentrated supernatant of Dendrobium officinale to ethanol is 1:4.5; the ethanol is anhydrous ethanol, and the low temperature refrigeration is refrigeration at 4°C; the centrifugation condition is at 8000 r / min for 12 min;

[0058] The preparation method of the concentrated extract of dendrobium residue comprises the following steps:

[0059] S1. After adding deionized water to the waste residue of Dendrobium, ultrasonic treatment is performed, cellulase and ligninase are added, and after enzymolysis, heating is performed to obtain a Dendrobium residue extract; wherein the dosage ratio of the waste residue of Dendrobium to deionized water is 1kg:10L, the ultrasonic treatment is performed at 120w for 25min; the mass ratio of the waste residue of Dendrobium, cellulase and ligninase is 10:0.2:0.08, and the enzymolysis is performed for 15min; the heating condition is heating to 95°C;

[0060] S2. Concentrating the dendrobium residue extract under reduced pressure to obtain a dendrobium residue concentrated extract; wherein the reduced pressure concentration condition is to concentrate at 80° C., and the dosage ratio of the dendrobium residue concentrated extract to the dendrobium officinale is 5L:1kg.

[0061] Comparative Example 1

[0062] Compared with Example 3, the difference is that comparative example 1 uses lactic acid bacteria instead of saccharomyces cerevisiae, and the other components, preparation steps and parameters are the same.

[0063] Comparative Examples 2-4

[0064] Compared with Example 3, the difference is that the total amount of plant extracts in Comparative Examples 2-4 remains unchanged, and the amounts of Eryngium littoralis extract, turmeric extract and rose callus extract are as shown in Table 1, and the other components, preparation steps and parameters are the same.

[0065] Table 1

[0066]

[0067] Comparative Example 5

[0068] Compared with Example 3, the difference is that in Comparative Example 5, no concentrated extract of dendrobium residue is used in step 1), and the other components, preparation steps and parameters are the same.

[0069] Comparative Example 6

[0070] Compared with Example 3, the difference is that in Comparative Example 6, glucose and ammonium sulfate are not used in step 1), and the other components, preparation steps and parameters are the same.

[0071] Comparative Examples 7-10

[0072] Compared with Example 3, the difference is that the total amount of the plant polysaccharide composition prepared in Comparative Examples 7-10 remains unchanged, and the amounts of Dendrobium extract, Licorice flavonoids, Glycyrrhizic acid and mixed fermentation product are as shown in Table 2, and the other components, preparation steps and parameters are the same.

[0073] Table 2

[0074] Dendrobium extract Licorice flavonoids Glycyrrhetinic acid Mixed fermentation Comparative Example 7 0 0.5 0.5 4 Comparative Example 8 5.6 0 0.5 4 Comparative Example 9 5.6 0.5 0 4 Comparative Example 10 5.6 0.5 0.5 0

[0075] The plant polysaccharide compositions prepared in Examples 1-3 and Comparative Examples 1-10 were subjected to melanin inhibition test and DPPH free radical scavenging test to obtain melanin inhibition rate and DPPH free radical scavenging rate. The test results are shown in Table 3.

[0076] Table 3

[0077] Melanin inhibition rate (%) DPPH free radical scavenging rate (%) Example 1 33.39 81.29 Example 2 32.68 80.60 Example 3 33.10 81.42 Comparative Example 1 27.82 77.64 Comparative Example 2 14.93 42.56 Comparative Example 3 10.42 38.65 Comparative Example 4 12.32 40.22 Comparative Example 5 25.26 72.58 Comparative Example 6 23.10 68.50 Comparative Example 7 9.32 34.24 Comparative Example 8 8.85 31.84 Comparative Example 9 13.14 42.97 Comparative Example 10 7.97 30.83

[0078] From the test results in Table 3, it can be seen that compared with Comparative Examples 1-10, the plant polysaccharide compositions prepared in Examples 1-3 of the present invention have significantly better melanin inhibition ability and DPPH free radical scavenging ability than Comparative Examples 1-10, that is, the plant polysaccharide compositions of the present invention have excellent whitening effects.

[0079] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A whitening plant polysaccharide composition, characterized in that: The preparation method of the plant polysaccharide composition comprises the following steps: 1) adding a bacterial agent to the mixed extract for fermentation, and obtaining a mixed fermentation product after ultrasonic treatment; the mixed extract comprises a plant extract, a concentrated extract of dendrobium residue, glucose and ammonium sulfate; the bacterial agent is saccharomyces cerevisiae; the mass ratio of the glucose, ammonium sulfate, the concentrated extract of dendrobium residue and the plant extract is 0.1-0.5:0.1-0.3:18-20:70-75; the plant extract is prepared by mixing a Eryngium littoralis extract, a turmeric extract and a rose callus extract in a mass ratio of 2:0.4-0.8:0.8-1.2; 2) homogenizing egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product to obtain a plant polysaccharide composition; the mass ratio of the egg yolk lecithin, cholesterol, polyglycerol-10, butylene glycol, dendrobium extract, glycyrrhizin, glycyrrhizinic acid, deionized water and mixed fermentation product is 1: 0.05-0.125: 0.3-0.8: 1.0-4.5: 2.0-5.6: 0.15-0.5: 0.25-0.50: 15-25: 1-4; Wherein, the preparation method of the dendrobium extract comprises the following steps: (1) crushing and sieving the Dendrobium officinale, adding deionized water, extracting and treating the mixture multiple times, centrifuging the mixture, and combining the supernatants to obtain a Dendrobium officinale supernatant; (2) concentrating the dendrobium supernatant under reduced pressure to obtain a dendrobium concentrated supernatant; (3) adding ethanol to the concentrated supernatant of Dendrobium officinale, refrigerating at low temperature, and discarding the supernatant after centrifugation to obtain a Dendrobium officinale extract; The preparation method of the concentrated extract of dendrobium residue comprises the following steps: S1. After adding deionized water to the waste residue of Dendrobium, ultrasonic treatment is performed, cellulase and ligninase are added, and after enzymolysis, heating is performed to obtain a Dendrobium residue extract; S2, concentrating the extract of dendrobium residue under reduced pressure to obtain a concentrated extract of dendrobium residue.

2. A whitening plant polysaccharide composition according to claim 1, characterized in that: In step 1), the mixed extract is sterilized by ultraviolet light before fermentation; the number of inoculated bacteria in the mixed extract is 10 5 -10 6 CFU / mL, the fermentation condition is fermentation at 25-35°C for 30-48h; the ultrasonic treatment is at 80-140w for 10-20min.

3. A whitening plant polysaccharide composition according to claim 1, characterized in that: In step (1), the sieving is through a 40-mesh sieve, the dosage ratio of the Dendrobium officinale and deionized water is 1 g: 25-28 mL; the multiple extraction treatment is extraction at 70-80° C. for 2-3 times; and the centrifugation condition is centrifugation at a speed of 5000-6000 r / min for 8-12 min.

4. The whitening plant polysaccharide composition according to claim 1, characterized in that: In step (1), the method further includes combining the waste residues after centrifugation to obtain the Dendrobium waste residues.

5. The whitening plant polysaccharide composition according to claim 1, characterized in that: In step (2), the reduced pressure concentration condition is to concentrate at 55-60°C.

6. The whitening plant polysaccharide composition according to claim 1, characterized in that: In step (3), the volume ratio of the concentrated supernatant of Dendrobium officinale to ethanol is 1:4.0-4.5; the ethanol is anhydrous ethanol, and the low-temperature refrigeration is refrigeration at 4°C; the centrifugation condition is 7500-8000r / min, and centrifugation for 8-12min.

7. The whitening plant polysaccharide composition according to claim 1, characterized in that: In step S1, the dosage ratio of the dendrobium waste residue and deionized water is 1kg:8-10L, the ultrasonic treatment is at 100-120w, and the ultrasonic treatment is for 15-25min; the mass ratio of the dendrobium waste residue, cellulase and ligninase is 10:0.1-0.2:0.06-0.08, and the enzymatic hydrolysis is for 10-15min; the heating condition is heating to 85-95°C; in step S2, the reduced pressure concentration condition is concentrating at 60-80°C, and the dosage ratio of the dendrobium residue concentrated extract to Dendrobium officinale is 3-5L:1kg.

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