A traditional Chinese medicine composition with the functions of improving immunity and tonifying qi and anti-aging and application thereof

Abstract

The application provides a traditional Chinese medicine composition with the functions of improving immunity and tonifying qi and resisting aging and application thereof. The traditional Chinese medicine composition comprises the following components in mass fractions: green tea 2255 parts, Gynostemma pentaphyllum 1000 parts, Astragalus extract 625 parts, Lycium barbarum fruit extract 600 parts, Ligustrum lucidum fruit extract 420 parts and Ganoderma lucidum extract 100 parts. The traditional Chinese medicine composition has the functions of improving immunity and tonifying qi and resisting aging through the synergistic effect of the multiple components.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care products, and particularly relates to a Chinese medicine composition capable of improving immunity, replenishing qi and resisting aging, and application thereof. Background Art

[0002] Immunity is a physiological function of the human body that distinguishes "self" from "non-self" components. It then destroys and rejects antigens (such as pathogens) that enter the body, as well as damaged cells and tumor cells produced by the body itself. This maintains health and resists or prevents infection by microorganisms, parasites, and other unwanted organisms. Qi and blood are essential for the proper functioning of tissues, organs, and systems. Replenishing Qi is one method of strengthening Qi and blood. In Traditional Chinese Medicine, Qi can be replenished through traditional Chinese medicine. This can enhance the body's immunity and abilities, strengthen the body's defenses, and reduce the risk of disease. It can also improve skin condition, regulate endocrine function, enhance mood and mental state, and improve sleep quality. With improvements in living standards, enhancing immunity and replenishing Qi for anti-aging benefits have become hot topics of research. Summary of the Invention

[0003] The purpose of the present invention is to provide a Chinese medicine composition having the functions of improving immunity, replenishing qi and resisting aging and its application.

[0004] In order to achieve the above objectives, the following technical solutions are provided:

[0005] A traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging comprises the following components in parts by mass: 2255 parts of green tea, 1000 parts of gynostemma pentaphyllum, 625 parts of astragalus root extract, 600 parts of wolfberry fruit extract, 420 parts of privet fruit extract and 100 parts of ganoderma lucidum extract.

[0006] Furthermore, the preparation method of Gynostemma pentaphyllum is as follows: fresh Gynostemma pentaphyllum leaves are selected, the pot temperature is controlled at 120°C, the fresh Gynostemma pentaphyllum leaves are put into the pot and stirred continuously; after 5 minutes, kneading is started to knead out 70% of the moisture in the fresh Gynostemma pentaphyllum leaves, the leaves are spread over a low fire for 20 minutes, and then dried over a high fire, turned over every 4 minutes, and baked for 40 minutes to obtain Gynostemma pentaphyllum.

[0007] Furthermore, the preparation method of the Ligustrum lucidum extract comprises the following steps:

[0008] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0009] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:5-7 and an ethanol aqueous solution with a mass fraction of 50-60%, perform ultrasonic extraction three times, each time for 2-4 hours, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0010] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0011] Furthermore, the step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 6-8 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 60-70° C. for 4-6 hours, and then crushing the fruit through a 100-mesh sieve.

[0012] Furthermore, the elution rate in step (3) is 1.5 BV / h.

[0013] Furthermore, the conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 30-40 KHz and temperature of 40-60°C.

[0014] Furthermore, the preparation method of the Ganoderma lucidum extract comprises the following steps:

[0015] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0016] (2) Ganoderma lucidum powder and water in a mass ratio of 1:10-12 are mixed, and continuous countercurrent ultrasonic extraction is performed, and solid-liquid separation is performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:10-12 are mixed, and continuous countercurrent ultrasonic extraction is performed, and solid-liquid separation is performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B are mixed to obtain a mixed extract;

[0017] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0018] Furthermore, the conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time of 80-90 min, temperature of 55-60° C., and ultrasonic frequency of 35-40 kHz.

[0019] Furthermore, the filter membrane in step (3) is a 0.22 μm filter membrane.

[0020] Furthermore, the preparation method of the Chinese medicine composition comprises the following steps:

[0021] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0022] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0023] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0024] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0025] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0026] Unless otherwise specified, other raw materials of the present invention are commercially available and are not particularly limited.

[0027] The present invention also provides an application of a Chinese medicine composition capable of improving immunity, replenishing qi and resisting aging.

[0028] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: the present invention provides a Chinese medicine composition and its application that can improve immunity, replenish qi and resist aging. The Chinese medicine composition provided by the present invention works synergistically through the compounding of multiple components, and has the effects of improving immunity, replenishing qi and resisting aging. The active ingredient extraction rate and retention rate of the glossy privet extract and the ganoderma lucidum extract of the present invention are high, and can improve immunity, enhance physical strength and prevent diseases. The ganoderma lucidum extract contains multiple active ingredients, such as polysaccharides, triterpenoids, etc., which can regulate the human immune system, enhance the body's immunity, and improve disease resistance. The triterpenoids in the ganoderma lucidum extract can enhance muscle strength and endurance, and improve the body's athletic ability; the multiple active ingredients in the glossy privet extract have obvious antioxidant effects, which can reduce the damage of free radicals to cells, delay aging, and prevent diseases. The polysaccharides in the glossy privet can enhance immunity, improve the body's resistance, and have certain effects on preventing colds and strengthening physical fitness. The above two components are compounded with the other multiple components in the system of the present invention to synergize and enhance the effect. DETAILED DESCRIPTION

[0029] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0030] Example 1

[0031] This embodiment provides a traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging, comprising the following components in parts by mass: 2255 parts of green tea, 1000 parts of Gynostemma pentaphyllum, 625 parts of Astragalus membranaceus extract, 600 parts of Lycium barbarum extract, 420 parts of Ligustrum lucidum extract, and 100 parts of Ganoderma lucidum extract.

[0032] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0033] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0034] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0035] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:6 and a 55% ethanol aqueous solution, perform ultrasonic extraction three times, each time for 3 h, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0036] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0037] The step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 7 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 65° C. for 5 hours, and then crushing the fruit through a 100-mesh sieve.

[0038] The elution rate in step (3) is 1.5 BV / h.

[0039] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 35 KHz and temperature of 50°C.

[0040] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0041] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0042] (2) Ganoderma lucidum powder and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B were mixed to obtain a mixed extract;

[0043] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0044] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time 85 min, temperature 57° C., and ultrasonic frequency 37 kHz.

[0045] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0046] The preparation method of the Chinese medicine composition comprises the following steps:

[0047] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0048] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0049] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0050] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0051] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0052] Example 2

[0053] This embodiment provides a traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging, comprising the following components in parts by mass: 2255 parts of green tea, 1000 parts of Gynostemma pentaphyllum, 625 parts of Astragalus membranaceus extract, 600 parts of Lycium barbarum extract, 420 parts of Ligustrum lucidum extract, and 100 parts of Ganoderma lucidum extract.

[0054] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0055] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0056] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0057] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:5 and a 50% ethanol aqueous solution, perform ultrasonic extraction three times, each time for 2 h, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0058] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0059] The step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 6 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 60° C. for 4 hours, and then crushing the fruit through a 100-mesh sieve.

[0060] The elution rate in step (3) is 1.5 BV / h.

[0061] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 30 KHz and temperature of 40°C.

[0062] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0063] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0064] (2) Ganoderma lucidum powder and water in a mass ratio of 1:10 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:10 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B were mixed to obtain a mixed extract;

[0065] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0066] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time of 80 min, temperature of 55° C., and ultrasonic frequency of 35 kHz.

[0067] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0068] The preparation method of the Chinese medicine composition comprises the following steps:

[0069] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0070] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0071] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0072] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0073] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0074] Example 3

[0075] This embodiment provides a traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging, comprising the following components in parts by mass: 2255 parts of green tea, 1000 parts of Gynostemma pentaphyllum, 625 parts of Astragalus membranaceus extract, 600 parts of Lycium barbarum extract, 420 parts of Ligustrum lucidum extract, and 100 parts of Ganoderma lucidum extract.

[0076] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0077] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0078] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0079] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:7 and a 60% ethanol aqueous solution, perform ultrasonic extraction three times, each time for 4 hours, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0080] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0081] The step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 8 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 70° C. for 6 hours, and then crushing the fruit through a 100-mesh sieve.

[0082] The elution rate in step (3) is 1.5 BV / h.

[0083] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 40 KHz and temperature of 60°C.

[0084] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0085] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0086] (2) Ganoderma lucidum powder and water in a mass ratio of 1:12 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:12 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B were mixed to obtain a mixed extract;

[0087] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0088] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time of 90 min, temperature of 60° C., and ultrasonic frequency of 40 kHz.

[0089] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0090] The preparation method of the Chinese medicine composition comprises the following steps:

[0091] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0092] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0093] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0094] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0095] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0096] Comparative Example 1

[0097] The difference between this comparative example and Example 1 is that the ratio of the components is different.

[0098] A traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging comprises the following components in parts by mass: 2000 parts of green tea, 1500 parts of gynostemma pentaphyllum, 525 parts of astragalus root extract, 700 parts of wolfberry fruit extract, 320 parts of privet fruit extract and 200 parts of ganoderma lucidum extract.

[0099] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0100] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0101] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0102] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:6 and a 55% ethanol aqueous solution, perform ultrasonic extraction three times, each time for 3 h, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0103] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0104] The step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 7 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 65° C. for 5 hours, and then crushing the fruit through a 100-mesh sieve.

[0105] The elution rate in step (3) is 1.5 BV / h.

[0106] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 35 KHz and temperature of 50°C.

[0107] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0108] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0109] (2) Ganoderma lucidum powder and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B were mixed to obtain a mixed extract;

[0110] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0111] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time 85 min, temperature 57° C., and ultrasonic frequency 37 kHz.

[0112] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0113] The preparation method of the Chinese medicine composition comprises the following steps:

[0114] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0115] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0116] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0117] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0118] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0119] Comparative Example 2

[0120] The difference between this comparative example and Example 1 is that the extract of Ligustrum lucidum fruit was commercially available from Hebei Jiuyu Biotechnology Co., Ltd.

[0121] A traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging comprises the following components in parts by mass: 7 parts of green tea, 8 parts of gynostemma pentaphyllum, 10 parts of astragalus root extract, 6 parts of wolfberry fruit extract, 8 parts of privet fruit extract, 9 parts of ganoderma lucidum extract, 4 parts of rhodiola rosea extract, 3 parts of snow lotus extract, 5 parts of dandelion extract, 3 parts of peony bark extract, 6 parts of knotweed extract, 2 parts of rehmannia root extract and 2 parts of cornus officinalis extract.

[0122] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0123] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0124] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0125] (2) Ganoderma lucidum powder and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:11 were mixed, and continuous countercurrent ultrasonic extraction was performed, and solid-liquid separation was performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B were mixed to obtain a mixed extract;

[0126] (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

[0127] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time 85 min, temperature 57° C., and ultrasonic frequency 37 kHz.

[0128] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0129] The preparation method of the Chinese medicine composition comprises the following steps:

[0130] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0131] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0132] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0133] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0134] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0135] Comparative Example 3

[0136] The difference between this comparative example and Example 1 is that the Ganoderma lucidum extract was purchased from Hebei Jiuyu Biotechnology Co., Ltd.

[0137] A traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging comprises the following components in parts by mass: 7 parts of green tea, 8 parts of gynostemma pentaphyllum, 10 parts of astragalus root extract, 6 parts of wolfberry fruit extract, 8 parts of privet fruit extract, 9 parts of ganoderma lucidum extract, 4 parts of rhodiola rosea extract, 3 parts of snow lotus extract, 5 parts of dandelion extract, 3 parts of peony bark extract, 6 parts of knotweed extract, 2 parts of rehmannia root extract and 2 parts of cornus officinalis extract.

[0138] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0139] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0140] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0141] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:6 and a 55% ethanol aqueous solution, perform ultrasonic extraction three times, each time for 3 h, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0142] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0143] The step (1) further comprises cleaning the Ligustrum lucidum fruit, steaming the fruit in water for 7 hours, taking out the Ligustrum lucidum fruit, drying the fruit at 65° C. for 5 hours, and then crushing the fruit through a 100-mesh sieve.

[0144] The elution rate in step (3) is 1.5 BV / h.

[0145] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 35 KHz and temperature of 50°C.

[0146] The preparation method of the Chinese medicine composition comprises the following steps:

[0147] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0148] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0149] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0150] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0151] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0152] Comparative Example 4

[0153] The difference between this comparative example and Example 1 is that the preparation methods of the Ligustrum lucidum extract and the Ganoderma lucidum extract are different.

[0154] A traditional Chinese medicine composition for improving immunity, replenishing qi and resisting aging comprises the following components in parts by mass: 7 parts of green tea, 8 parts of gynostemma pentaphyllum, 10 parts of astragalus root extract, 6 parts of wolfberry fruit extract, 8 parts of privet fruit extract, 9 parts of ganoderma lucidum extract, 4 parts of rhodiola rosea extract, 3 parts of snow lotus extract, 5 parts of dandelion extract, 3 parts of peony bark extract, 6 parts of knotweed extract, 2 parts of rehmannia root extract and 2 parts of cornus officinalis extract.

[0155] The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; starting to knead the leaves after 5 minutes to knead out 70% of the moisture in the leaves, spreading them over a low fire for 20 minutes, and then drying them over a high fire, turning them over every 4 minutes, and baking them for 40 minutes to obtain Gynostemma pentaphyllum.

[0156] The preparation method of the Ligustrum lucidum fruit extract comprises the following steps:

[0157] (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 300-mesh sieve to obtain Ligustrum lucidum fruit powder;

[0158] (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:10 and a 70% ethanol aqueous solution, perform ultrasonic extraction twice, each time for 2 h, combine the two filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract;

[0159] (3) The extract was dispersed in water and eluted with AB-8 macroporous resin in sequence with 5 column volumes of water and 5 column volumes of 70 wt% ethanol aqueous solution. The eluate was collected, the ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract.

[0160] The elution rate in step (3) is 2 BV / h.

[0161] The conditions for ultrasonic extraction in step (2) are: ultrasonic frequency of 20 KHz and temperature of 35°C.

[0162] The preparation method of the Ganoderma lucidum extract comprises the following steps:

[0163] (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through a 300-mesh sieve to obtain Ganoderma lucidum powder;

[0164] (2) Ganoderma lucidum powder and water were mixed in a mass ratio of 1:6, and continuous countercurrent ultrasonic extraction was performed, followed by solid-liquid separation to obtain Ganoderma lucidum extract and Ganoderma lucidum residue;

[0165] (3) The Ganoderma lucidum extract is concentrated through a filter membrane and dried to obtain the Ganoderma lucidum extract.

[0166] The conditions for the continuous countercurrent ultrasonic extraction in step (2) are: extraction time of 180 min, temperature of 50° C., and ultrasonic frequency of 45 kHz.

[0167] The filter membrane in step (3) is a 0.22 μm filter membrane.

[0168] The preparation method of the Chinese medicine composition comprises the following steps:

[0169] (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside;

[0170] (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 67°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter.

[0171] (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film.

[0172] (4) Outer packaging: 10 bags per box, with labels and other outer packaging.

[0173] (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

[0174] Performance Testing

[0175] 1. Anti-fatigue function test:

[0176] The Chinese medicine compositions of Examples 1-3 and Comparative Examples 1-4 were bagged according to the formulation, at 5.0 portions per bag. After soaking in 100 mL of 100°C water for 30 minutes, the resulting liquid was administered orally to test animals twice daily at a volume of 0.1 mL / 10 portions / bw. The control group received the same volume of water. Each group received gavage for 30 consecutive days. 30 minutes after the last administration, a lead weight of 5% of the mouse's body weight was placed at the base of the mouse's tail. The mice were placed in a swimming tank with a water depth of 30 cm and a temperature of 25°C ± 1.0°C. The weighted swimming time (s) of the mice was recorded, which is the time from the start of swimming to death.

[0177] Table 1 Test results

[0178]

[0179] The results show that the Chinese medicine compositions of Examples 1-3 have good effects of replenishing qi and strengthening physical fitness. Comparative Examples 1-3 show that the effects decrease to varying degrees when the composition and preparation method are changed.

[0180] 2. Test to enhance immune function

[0181] Male Kunming mice weighing 20-24 kg were randomly divided into 7 groups of 10 mice each. The Chinese medicine compositions of Examples 1-3 and Comparative Examples 1-4 were bagged according to their respective proportions (5.0 portions / bag). After soaking in 100 mL of 100°C water for 30 minutes, the resulting solution was administered orally twice daily at a volume of 0.1 mL / 10 portions / kg. A control group received the same volume of water. This was continued orally for 30 consecutive days. Thirty minutes after the last dose, each mouse was sensitized with an intraperitoneal injection of 0.2 mL of 2% (v / v) SRBC. Four days later, the left hind paw thickness was measured. Then, 20% (v / v) SRBC (20 μL / mouse) was subcutaneously injected at the measurement site. 24 hours after injection, the left hind paw thickness was measured. Three measurements were taken at the same site, and the average value was calculated. The difference in paw thickness before and after challenge (the degree of paw swelling) was used to represent the degree of DTH.

[0182] Table 2 Test results

[0183]

[0184] The results show that the Chinese medicine compositions of Examples 1-3 have better immunity-enhancing effects. Comparative Examples 1-3 show that the effects decrease to varying degrees when the compositions and preparation methods are changed.

[0185] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for improving immunity, replenishing qi and resisting aging, characterized in that: The invention is composed of the following components in parts by mass: 2255 parts of green tea, 1000 parts of Gynostemma pentaphyllum, 625 parts of Astragalus root extract, 600 parts of Lycium barbarum fruit extract, 420 parts of Ligustrum lucidum fruit extract and 100 parts of Ganoderma lucidum extract; The preparation method of Gynostemma pentaphyllum comprises the following steps: selecting fresh Gynostemma pentaphyllum leaves, controlling the pot temperature at 120° C., adding the fresh Gynostemma pentaphyllum leaves into the pot and stirring continuously; kneading the leaves after 5 minutes to remove 70% of the moisture in the leaves, spreading the leaves over a low fire for 20 minutes, and then drying the leaves over a high fire, turning the leaves over every 4 minutes, and baking the leaves for 40 minutes to obtain Gynostemma pentaphyllum; The preparation method of the Ligustrum lucidum fruit extract comprises the following steps: (1) Clean the Ligustrum lucidum fruit, dry and crush it, and pass it through a 100-mesh sieve to obtain Ligustrum lucidum fruit powder; (2) Mix the powder of Ligustrum lucidum fruit with a mass ratio of 1:5-7 and an ethanol aqueous solution with a mass fraction of 50-60%, perform ultrasonic extraction three times, each time for 2-4 hours, combine the three filtrates, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain the extract; (3) The extract was dispersed in water and eluted using AB-8 macroporous resin with 5 column volumes of water, 6 column volumes of 30 wt% ethanol aqueous solution, and 7 column volumes of 60 wt% ethanol aqueous solution. The eluate was collected, ethanol was recovered under reduced pressure, and freeze-dried to obtain the Ligustrum lucidum fruit extract; The preparation method of Ganoderma lucidum extract comprises the following steps: (1) Dry and crush the Ganoderma lucidum fruiting body, and pass it through an 80-mesh sieve to obtain Ganoderma lucidum powder; (2) Ganoderma lucidum powder and water in a mass ratio of 1:10-12 are mixed, and continuous countercurrent ultrasonic extraction is performed, and solid-liquid separation is performed to obtain Ganoderma lucidum extract A and Ganoderma lucidum residue; Ganoderma lucidum residue and water in a mass ratio of 1:10-12 are mixed, and continuous countercurrent ultrasonic extraction is performed, and solid-liquid separation is performed to obtain Ganoderma lucidum extract B; Ganoderma lucidum extract A and Ganoderma lucidum extract B are mixed to obtain a mixed extract; (3) The mixed extract is concentrated through a filter membrane and dried to obtain a Ganoderma lucidum extract.

2. The Chinese medicine composition for improving immunity, replenishing qi and resisting aging according to claim 1, characterized in that: The preparation method of the Ligustrum lucidum fruit extract further comprises the steps of: cleaning the Ligustrum lucidum fruit, steaming the Ligustrum lucidum fruit in water for 6-8 hours, taking out the Ligustrum lucidum fruit, drying the Ligustrum lucidum fruit at 60-70° C. for 4-6 hours, and then crushing the Ligustrum lucidum fruit through a 100-mesh sieve.

3. The Chinese medicine composition for improving immunity, replenishing qi and resisting aging according to claim 2, characterized in that: The elution rate in step (3) of the preparation method of the Ligustrum lucidum extract is 1.5 BV / h.

4. The Chinese medicine composition for improving immunity, replenishing qi and resisting aging according to claim 3, characterized in that: The conditions for ultrasonic extraction in step (2) of the preparation method of the Ligustrum lucidum fruit extract are: ultrasonic frequency of 30-40 KHz and temperature of 40-60°C.

5. The Chinese medicine composition for improving immunity, replenishing qi and resisting aging according to claim 4, characterized in that: The conditions for continuous countercurrent ultrasonic extraction in step (2) of the preparation method of the Ganoderma lucidum extract are: extraction time of 80-90 min, temperature of 55-60° C., and ultrasonic frequency of 35-40 kHz.

6. The Chinese medicine composition for improving immunity, replenishing qi and resisting aging according to claim 5, characterized in that: The preparation method of the Chinese medicine composition comprises the following steps: (1) Pass the wolfberry extract, ganoderma lucidum extract, astragalus extract, and glossy privet extract through a 60-mesh sieve, crush the gynostemma pentaphyllum and green tea, and pass through a 60-mesh sieve respectively; weigh each raw material and set aside; (2) Mixing, granulation, drying, and granulation: Mix all the raw materials evenly, then add 70% ethanol for wet granulation to obtain wet granules; dry the wet granules at a drying temperature of 65-70°C, and sieve the obtained dry granules with a 14-mesh sieve; the dry granules should be dried evenly and free of other impurities and foreign matter. (3) Inner packaging: The dry granules obtained above were divided into filter paper bags, and the amount of each bag was adjusted to 5g; then the filter paper bags were wrapped with composite film. (4) Outer packaging: 10 bags per box, with labels and other outer packaging. (5) Finished product inspection and warehousing: Each batch of products shall be sampled for inspection in accordance with the enterprise standard of this product, and qualified products shall be put into warehousing.

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