A method for removing aspergillus niger spores by synergistically strengthening ultraviolet auxiliary micro-current catalytic potassium ferrate and ozone water

Abstract

The application discloses a method for removing aspergillus niger spores by synergistically strengthening ultraviolet auxiliary micro-current catalysis potassium ferrate and ozone water, and relates to a method for removing aspergillus niger spores. The application is a technology for enhancing the sterilization effect by combining micro-current catalysis activation of potassium ferrate with ozone water and utilizing ultraviolet irradiation. The method aims to solve the problems of insufficient killing efficiency of existing sterilization technologies on high-resistance spores and high risk of sterilization by-products. The specific steps include: adding potassium ferrate into water containing aspergillus niger spores; micro-current catalysis, and disconnecting the current after the catalysis is completed; introducing ozone into the catalyzed water, and then performing ultraviolet irradiation to enhance the sterilization activity and effectively improve the killing rate of aspergillus niger spores. The application can realize a killing rate of aspergillus niger spores of more than 98.3%, significantly reduce the generation of sterilization by-products, and has the advantages of simple operation, environmental protection and safety.

Description

Technical Field

[0001] This invention relates to a method for removing Aspergillus niger spores. Background Technology

[0002] When treating raw water, in addition to removing inorganic and organic pollutants, it is also necessary to pay attention to pathogenic microorganisms such as Aspergillus niger, which have strong pathogenicity and reproductive capabilities. These microorganisms pose a serious threat to human health, and traditional disinfection technologies such as chlorine disinfection, chlorine dioxide disinfection, sodium hypochlorite disinfection, ultraviolet irradiation, and ozone disinfection are often ineffective in eliminating highly resistant microorganisms that produce vegetative and dormant forms. Furthermore, these traditional methods have high water quality requirements, are costly, and may produce harmful disinfection byproducts such as trihalomethanes and haloacetic acids, further exacerbating environmental pollution. Therefore, developing a more effective disinfection method is crucial to solving this challenge in order to improve water treatment efficiency and reduce environmental impact.

[0003] Aspergillus niger, a common species in the genus Aspergillus, is a prevalent filamentous fungus widely distributed in the natural environment, especially in soil, air, and decaying plant material. It is an important microorganism in industrial production, widely used in enzyme production and the fermentation of organic acids (such as citric acid). However, Aspergillus niger is also a recognized pathogenic fungus; the widespread distribution and high survival rate of its spores pose a significant health risk in hospitals, laboratories, and homes.

[0004] Aspergillus niger produces a large number of spores that are highly resilient, able to survive in extreme environments, and can be spread through the air. Inhaling air containing Aspergillus niger spores can lead to respiratory illnesses such as allergic bronchopulmonary aspergillosis and aspergillosis. Furthermore, in individuals with weakened immune systems, such as cancer patients, organ transplant recipients, or HIV / AIDS patients, Aspergillus niger may cause more severe invasive fungal infections.

[0005] Existing disinfection indicator bacteria mostly use Bacillus subtilis, but Aspergillus niger spores are much larger in morphology than Bacillus subtilis sporosomes and have higher tolerance. Traditional disinfection methods (such as simple physical irradiation or chemical treatment) often fail to completely kill its spores, and all have shortcomings and defects, and may produce harmful disinfection byproducts, such as trihalomethanes, chlorophenols, and other organochlorine compounds. Currently, few disinfection methods can simultaneously meet the requirements of applicability to various aquatic environments, high efficiency in sterilization, ease of operation, and low cost. Therefore, developing a safe, efficient, and environmentally friendly disinfection method is crucial for controlling the spread of Aspergillus niger spores. Summary of the Invention

[0006] The purpose of this invention is to address the problems of traditional disinfection methods, such as low kill rate of pathogenic microorganisms in water, easy generation of carcinogenic, teratogenic, and mutagenic substances, small applicable pH range, high requirements for water turbidity, large consumption of reagents and strict storage conditions, environmental unfriendliness, and inability to achieve low cost and easy operation. The invention provides a method for the synergistic enhancement of removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis of potassium ferrate and ozone water.

[0007] A method for the synergistic enhancement of potassium ferrate and ozone water removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis is specifically carried out according to the following steps:

[0008] 1. Add potassium ferrate to water containing Aspergillus niger spores to obtain water containing both potassium ferrate and Aspergillus niger spores;

[0009] 2. A microcurrent is passed through water containing potassium ferrate and Aspergillus niger spores for a period of time to carry out catalytic treatment;

[0010] 3. After power is cut off, ozone is continuously introduced into the catalytically treated water, and ultraviolet light is used for irradiation to obtain water in which Aspergillus niger spores are removed.

[0011] The principles and advantages of this invention:

[0012] This invention provides a method for the synergistic enhancement of potassium ferrate and ozone water in the removal of Aspergillus niger spores through ultraviolet-assisted microcurrent catalysis. The aim is to utilize the strong oxidizing properties of potassium ferrate and ozone, synergistically using microcurrent to catalyze potassium ferrate removal, while simultaneously utilizing ultraviolet (UV) light... 254 It helps to kill pathogenic microorganisms and reduce the concentration of Aspergillus niger spores in the water;

[0013] Firstly, the technical principle of this invention is as follows: Potassium ferrate in aqueous solution can release high-valence iron ions (Fe(VI)) that directly act on the protein components of microorganisms, destroying their cell walls, cell membranes, and other tissue structures, while reducing cytotoxicity and genotoxicity; microcurrent can promote a more complete and rapid oxidation reaction of potassium ferrate. Microcurrent increases the electronic activity in the solution, accelerating the valence state transition of iron ions, thereby enhancing the oxidant's attack on cell structures and increasing the kill rate; ozone, as another strong oxidant, can penetrate the cell wall of microorganisms, destroying the enzyme system inside the cell and blocking the metabolism of microorganisms. The addition of ozone can effectively eliminate any microorganisms that may remain after treatment with potassium ferrate; ultraviolet (UV) radiation... 254 It can not only directly destroy the DNA structure of microorganisms, preventing them from replicating and regenerating, but also activate ozone molecules in water, generating a high concentration of hydroxyl radicals and creating a highly efficient oxidation environment. This is especially effective for spore-form microorganisms that are difficult to remove by a single chemical method.

[0014] Secondly, compared with the prior art, the method of "ultraviolet-assisted microcurrent catalytic synergistic disinfection of Aspergillus niger spores by potassium ferrate and ozone water" proposed in this invention has the following advantages: The ultraviolet (UV) light added to the combined system of potassium ferrate, microcurrent, and ozone water in this invention... 254 Irradiation activates some of the ozone in the system, generating a large number of reactive free radicals, which enhances the reaction efficiency of ozone water and potassium ferrate, creating a highly efficient disinfection environment. Microcurrent catalysis precisely controls the rate and range of the chemical reaction, effectively reducing the generation of harmful chemical byproducts. This method is simple to operate, low in cost, suitable for large-scale water treatment, has minimal environmental impact, and meets environmental protection and sustainable development standards. Furthermore, this method is not only applicable to Aspergillus niger spores but can also control other microorganisms, offering broad application prospects. The simple and mild reaction process, easy operation and control, and applicability to various water bodies effectively solve pollution problems in raw water disinfection, demonstrating high practicality and innovation. Detailed Implementation

[0015] Specific Implementation Method 1: This implementation method describes a method for the synergistic enhancement of potassium ferrate and ozone water removal of Aspergillus niger spores through ultraviolet-assisted microcurrent catalysis, which is carried out according to the following steps:

[0016] 1. Add potassium ferrate to water containing Aspergillus niger spores to obtain water containing both potassium ferrate and Aspergillus niger spores;

[0017] 2. A microcurrent is passed through water containing potassium ferrate and Aspergillus niger spores for a period of time to carry out catalytic treatment;

[0018] 3. After power is cut off, ozone is continuously introduced into the catalytically treated water, and ultraviolet light is used for irradiation to obtain water in which Aspergillus niger spores are removed.

[0019] Specific Implementation Method Two: This implementation method differs from Specific Implementation Method One in that the dosage of potassium ferrate in step one is 8 mg / L to 15 mg / L. The other steps are the same as in Specific Implementation Method One.

[0020] Specific Implementation Method Three: This implementation method differs from Specific Implementation Method One or Two in that: the concentration of Aspergillus niger spores in the water containing Aspergillus niger spores in step one is 10. 6 CFU / mL ~10 7 CFU / mL. Other steps are the same as in specific implementation method one or two.

[0021] Specific Implementation Method Four: This implementation method differs from Specific Implementation Methods One to Three in that the current density of the microcurrent mentioned in step two is 0.5 mA / cm². 2 ~1mA / cm 2 The other steps are the same as those in implementation methods one through three.

[0022] Specific Implementation Method Five: This implementation method differs from Specific Implementation Methods One to Four in that the catalytic treatment time in step two is 15 to 30 minutes. The other steps are the same as in Specific Implementation Methods One to Four.

[0023] Specific Implementation Method Six: This implementation method differs from Specific Implementation Methods One to Five in that the ozone injection rate in step three is 30 g / h to 40 g / h. The other steps are the same as in Specific Implementation Methods One to Five.

[0024] Specific Implementation Method Seven: The difference between this implementation method and Specific Implementation Methods One through Six is ​​that the wavelength of the ultraviolet light mentioned in step three is 254nm, and the irradiation dose is 20mJ / cm². 2 ~40mJ / cm 2 The other steps are the same as those in Specific Implementation Methods One through Six.

[0025] Specific Implementation Method Eight: This implementation method differs from Specific Implementation Methods One to Seven in that the ultraviolet light irradiation time and ozone introduction time in step three are the same, ranging from 15 to 30 minutes. The other steps are the same as in Specific Implementation Methods One to Seven.

[0026] Specific Implementation Method Nine: This implementation method differs from Specific Implementation Methods One through Eight in that the concentration of Aspergillus niger spores in the water used to remove Aspergillus niger spores is 10. 4 CFU / mL ~ 1.7*10 5 CFU / mL. Other steps are the same as in specific implementation methods one through eight.

[0027] Specific Implementation Method Ten: The difference between this implementation method and Specific Implementation Methods One to Nine is that the removal rate of Aspergillus niger spores in step three is 98.3% to 99.1%. The other steps are the same as in Specific Implementation Methods One to Nine.

[0028] The beneficial effects of the present invention are verified using the following embodiments:

[0029] Example 1: A method for the synergistic enhancement of potassium ferrate and ozone water removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis is specifically carried out according to the following steps:

[0030] 1. Add potassium ferrate to water containing Aspergillus niger spores to obtain water containing both potassium ferrate and Aspergillus niger spores;

[0031] The dosage of potassium ferrate mentioned in step one is 8 mg / L;

[0032] The concentration of Aspergillus niger spores in the water mentioned in step one is 10. 6 CFU / mL;

[0033] 2. Pass a microcurrent through water containing potassium ferrate and Aspergillus niger spores for 15 minutes;

[0034] The current density of the microcurrent mentioned in step two is 1 mA / cm². 2 ;

[0035] 3. After power failure, ozone is continuously introduced into the catalytically treated water, and ultraviolet light is used for 0 min to 15 min. Samples are taken at specific time points of 0, 5, 10, and 15 min, and then plated to cultivate colonies. The concentration of Aspergillus niger spores is calculated. After reacting for 15 min, water with Aspergillus niger spores removed is obtained.

[0036] The ozone injection rate mentioned in step three is 40 g / h;

[0037] The ultraviolet light mentioned in step three has a wavelength of 254 nm and an irradiation dose of 20 mJ / cm². 2 .

[0038] In step three, the culture medium used for plating to cultivate colonies was Czapek-Dox agar; the culture temperature was 37℃ and the culture time was 48h.

[0039] Comparative Example 1: The difference between this embodiment and Example 1 is that the current density of the microcurrent mentioned in step two is 0 mA / cm². 2 The ozone injection rate in step three is 0 g / h; the ultraviolet light irradiation dose in step three is 0 mJ / cm². 2 The other steps and parameters are the same as in Example 1.

[0040] Comparative Example 2: The differences between this example and Example 1 are as follows: the dosage of potassium ferrate in step one is 0 mg / L; the ozone injection rate in step three is 0 g / h; and the ultraviolet light irradiation dose in step three is 0 mJ / cm². 2 The other steps and parameters are the same as in Example 1.

[0041] Comparative Example 3: The difference between this example and Example 1 is that the dosage of potassium ferrate in step one is 0 mg / L; the current density of the microcurrent in step two is 0 mA / cm². 2 The ultraviolet light irradiation dose mentioned in step three is 0 mJ / cm². 2 The other steps and parameters are the same as in Example 1.

[0042] Comparative Example 4: The difference between this example and Example 1 is that the dosage of potassium ferrate in step one is 0 mg / L; the current density of the microcurrent in step two is 0 mA / cm².2 The ozone injection rate in step three is 0 g / h. All other steps and parameters are the same as in Example 1.

[0043] Comparative Example 5: The difference between this example and Example 1 is that potassium ferrate is omitted in step one, i.e., the dosage of potassium ferrate in step one is 0 mg / L. All other steps and parameters are the same as in Example 1.

[0044] Comparative Example 6: The difference between this embodiment and Example 1 is that the use of microcurrent is omitted in step two; that is, the current density of the microcurrent mentioned in step two is 1 mA / cm². 2 The other steps and parameters are the same as in Example 1.

[0045] Comparative Example 7: The difference between this example and Example 1 is that ozone introduction is omitted in step three, i.e., the ozone introduction rate in step three is 0 g / h. All other steps and parameters are the same as in Example 1.

[0046] Comparative Example 8: The difference between this example and Example 1 is that ultraviolet light irradiation is omitted in step three, that is, the ultraviolet light irradiation dose in step three is 0 mJ / cm². 2 The other steps and parameters are the same as in Example 1.

[0047] Table 1 shows the removal rate of Aspergillus niger spores at different times in the reaction systems of Examples 1-8 (Comparative Examples 1-8).

[0048] Table 1

[0049]

[0050] As shown in Table 1, traditional single disinfection systems can kill a maximum of only 72.8% of Aspergillus niger spores within 15 minutes, while the killing effect of microcurrent and ultraviolet light alone on Aspergillus niger spores is relatively limited. This may be due to the slow sterilization rate when microcurrent or ultraviolet light is used alone. However, in the complete system of this invention, through the synergistic effect of electrocatalytic potassium ferrate and ozone, supplemented by ultraviolet (UV) light... 254 Activation with ozone significantly improved the killing efficiency against Aspergillus niger spores. Furthermore, the removal rate of Aspergillus niger spores reached approximately 98.4% within 15 minutes, demonstrating a significant killing effect. Comparative experimental results show that omitting any one of these factors led to a decrease in the removal rate. Microcurrent, ozone water, and ultraviolet (UV) light were used to activate the spores. 254 The combination of potassium ferrate, ozone water, and ultraviolet (UV) light achieved a removal rate of 79.1% within 15 minutes. While the effect was still significant, it was noticeably lower compared to the complete system. 254The combination of these two methods achieved a removal rate of 82.5% within 15 minutes, demonstrating effective synergistic action. However, the lack of microcurrent weakened the catalytic effect. Microcurrent catalysis of potassium ferrate synergistically with ultraviolet (UV) light... 254 Within 15 minutes, the removal rate decreased to 66.5%, demonstrating that the oxidizing effect of ozone is crucial for improving sterilization efficiency; the microcurrent-catalyzed potassium ferrate synergistic ozone removal rate reached 86% within 15 minutes, proving that ultraviolet (UV) radiation... 254 The activation of ozone to generate a large number of hydroxyl radicals and the catalytic effect of microcurrent are key factors in enhancing the overall sterilization efficiency.

Claims

1. A method for the synergistic enhancement of potassium ferrate and ozone water removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis, characterized in that... The method is specifically implemented according to the following steps: Step 1: Add potassium ferrate to water containing Aspergillus niger spores to obtain water containing potassium ferrate and Aspergillus niger spores. The dosage of potassium ferrate is 8 mg / L to 15 mg / L. Step 2: Catalytically treat the water containing potassium ferrate and Aspergillus niger spores by introducing a microcurrent for 15-30 minutes. The current density of the microcurrent is 0.5 mA / cm². 2 ~1mA / cm 2 ; Step 3: After power is cut off, ozone is continuously introduced into the catalytically treated water, while simultaneously irradiating it with ultraviolet light for 15-30 minutes to obtain water with Aspergillus niger spores removed. The ozone introduction rate is 30-40 g / h; the wavelength of the ultraviolet light is 254 nm, and the irradiation dose is 20 mJ / cm². 2 ~40mJ / cm 2 .

2. The method for the synergistic enhancement of potassium ferrate and ozone water in the removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis according to claim 1, characterized in that... The concentration of Aspergillus niger spores in the water mentioned in step one is 10. 6 CFU / mL ~10 7 CFU / mL.

3. The method for the synergistic enhancement of potassium ferrate and ozone water in the removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis according to claim 1, characterized in that... The concentration of Aspergillus niger spores in the water used to remove Aspergillus niger spores was 10. 4 CFU / mL ~1.7×10 5 CFU / mL.

4. The method for the synergistic enhancement of potassium ferrate and ozone water in the removal of Aspergillus niger spores by ultraviolet-assisted microcurrent catalysis according to claim 1, characterized in that... In step three, the removal rate of Aspergillus niger spores was 98.3%~99.1%.

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