Banana plant growth regulator composition and preparation method, application and banana plant growth regulator preparation

Abstract

The present invention relates to the field of plant growth technology, and specifically provides a banana plant regulator composition and a preparation method, application, and banana plant regulator preparation. The composition comprises: 5 to 15 parts of gibberellins, 1 to 5 parts of naphthoxyacetic acid, 2 to 5 parts of 14-hydroxybrassinosteroids, and 0.1 to 2 parts of arabinose. Compared with the prior art, applying the banana plant regulator composition of the present invention as the main component of foliar fertilizer can significantly increase the stem height, stem thickness, and chlorophyll content of banana plants, promote the growth of banana plants, and has broad development and application prospects.

Description

Technical Field

[0001] The invention relates to the field of plant growth technology, and more specifically to a banana plant regulator composition and a preparation method and application thereof, and a banana plant regulator preparation. Background Art

[0002] Banana (Musa spp) belongs to the Musaceae family and the Musa genus. It is an important food crop and cash crop in the world. When ripe, the banana flesh is soft and delicate, yellow-white, and has a sweet taste. Banana is rich in various vitamins and minerals, especially potassium, vitamin C and vitamin B6, and is also a good source of fiber.

[0003] Banana tissue culture seedlings are a method of propagating banana plants through tissue culture technology. This method is usually used to mass-produce disease-free, high-yield, high-quality banana seedlings to ensure the quality and quantity of the plants. Banana tissue culture seedlings cultivated through tissue culture technology have the following advantages: rapid propagation of a large number of high-quality disease-free plants; saving seeds and space, improving propagation efficiency; ensuring the quality and consistency of the plants.

[0004] Plant growth regulators are a class of chemical substances that can affect plant growth and development, usually including two types: plant hormones and growth inhibitors. They can affect the physiological processes of plants in various ways, thereby regulating plant growth, flowering, fruiting and the transport of nutrients. Common plant growth regulators include auxin, ethylene, cytokinin, etc. Plant growth regulators play an important role in modern agriculture and can be used to control crop growth, increase yield, improve quality, and extend the shelf life of fruits.

[0005] However, there is currently no specific plant growth regulator that can significantly promote the growth of banana tissue culture seedlings. Summary of the invention

[0006] In view of this, the object of the present invention is to provide a banana plant regulator composition and its preparation method, application, and banana plant regulator preparation. The banana plant regulator composition of the present invention can significantly increase the stem height, stem diameter and chlorophyll content of banana plants, and promote the growth of banana plants.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] The banana plant growth regulator composition comprises, by weight, 5 to 15 parts of gibberellins, 1 to 5 parts of naphthoxyacetic acid, 2 to 5 parts of 14-hydroxybrassinosteroids and 0.1 to 2 parts of arabinose.

[0009] Gibberellin acid (GA3) is a certain type of plant hormone. Gibberellin plays an important regulatory role in the growth and development of plants, especially in promoting seedling elongation, seed germination, pollen tube growth and fruit development. Gibberellin can promote cell division and elongation in plants, thereby affecting the morphology and structure of plants. Gibberellin is also involved in regulating the photosynthesis, nutrient absorption and metabolic processes of plants. Gibberellin has a multi-faceted effect on the growth and development of plants and plays an important role in plant growth and development and agricultural production.

[0010] The naphthoxyacetic acid of the present invention is 2-naphthoxyacetic acid (2-Naphthoxyacetic acid, BNOA), the molecular formula of which is C 12 H 10 O3, CAS No. 120-23-0, naphthoxyacetic acid is a naphthalene plant growth regulator with auxin biological activity, absorbed by leaves and roots, and can promote plant rooting; low content of 2-naphthoxyacetic acid has the effect of fruit setting. 2-naphthoxyacetic acid is used as a plant growth stimulant to increase the fruit setting rate and regulate the growth of pineapples, strawberries, and tomatoes.

[0011] Brassinosteroids are a general term for a class of polyhydroxylated sterol compounds that are widely found in plants. Brassinosteroids play a very important role in almost all plant growth and development processes and in the process of adapting to the environment. They are involved in regulating many growth and development processes such as plant photomorphogenesis, cell division and differentiation, reproductive development, flowering, aging, and the response of plants to adversity. 14-hydroxylated brassinosteroid is a type of brassinosteroid, also known as 14β-hydroxybrassinosteroid. It is an important biologically active compound that plays an important role in regulating growth and development, stress response, and other important physiological functions in plants.

[0012] The arabinose of the present invention is DL-arabinose, and the molecular formula is C5H 10 O5, CAS number is 147-81-9, melting point is 158°C. DL-arabinose is a white fine crystalline powder at room temperature; it is soluble in water and glycerol, and insoluble in ethanol and ether.

[0013] Preferably, the banana includes one or more of the following: Brazilian banana, pink banana, Baodao banana, and Nandina chinensis, preferably Brazilian banana.

[0014] Preferably, the banana vegetative regulator composition comprises, by weight: 6 to 10 parts of gibberellins, 1 to 3 parts of naphthoxyacetic acid, 2 to 4 parts of 14-hydroxybrassinosteroids and 0.6 to 2 parts of arabinose.

[0015] Preferably, the purity of the gibberellin is ≥90%; the naphthoxyacetic acid is 2-naphthoxyacetic acid; the purity of the naphthoxyacetic acid is ≥98%; the purity of the 14-hydroxybrassinosteroid is ≥98%; the arabinose is DL-arabinose; the purity of the arabinose is ≥98%.

[0016] In one embodiment of the present invention, the banana planting conditioning composition comprises, by weight:

[0017] 10 parts of gibberellins, 3 parts of naphthoxyacetic acid, 4 parts of 14-hydroxybrassinosteroids and 2 parts of arabinose.

[0018] In one embodiment of the present invention, the banana plant conditioning composition comprises, by weight:

[0019] 6 parts of gibberellins, 2 parts of naphthoxyacetic acid, 2 parts of 14-hydroxybrassinosteroids and 0.8 parts of arabinose.

[0020] In one embodiment of the present invention, the banana planting conditioning composition comprises, by weight:

[0021] 7 parts of gibberellins, 1 part of naphthoxyacetic acid, 2 parts of 14-hydroxybrassinosteroids and 0.6 parts of arabinose.

[0022] The present invention also provides a method for preparing the banana plant growth regulator composition, comprising the following steps:

[0023] Components including 5-15 parts of gibberellins, 1-5 parts of naphthoxyacetic acid, 2-5 parts of 14-hydroxybrassinosteroids and 0.1-2 parts of arabinose are mixed in parts by mass to obtain a banana plant growth regulator composition.

[0024] The invention also provides application of the banana plant growth regulator composition in promoting banana growth.

[0025] Preferably, the banana plant growth regulator composition is used for banana tissue culture seedlings; the banana tissue culture seedlings have a length of 18 cm to 22 cm and 8 to 10 leaves.

[0026] Preferably, the pseudostem thickness of the banana tissue culture seedling is ≥1 cm.

[0027] Preferably, the banana plant conditioning composition is used as foliar fertilizer or root fertilizer for banana tissue culture seedlings; when used as foliar fertilizer, it is sprayed on the surface of the leaves to cover the surface of the leaves with a layer of liquid. The concentration of the banana plant conditioning composition in the foliar fertilizer is preferably 10-20 mg / L, more preferably 10 mg / L or 20 mg / L, and the application amount of the foliar fertilizer is 15-25 mL / plant, more preferably 25 mL / plant; the banana plant conditioning preparation is applied once every 10-15 days and continuously applied for 4-6 months; preferably, 100-200 bananas are planted per mu, more preferably 150 bananas.

[0028] The invention also provides a banana plant regulator preparation, comprising the banana plant regulator composition and an auxiliary agent.

[0029] Preferably, the concentration of the banana plant growth regulator composition in the banana plant growth regulator preparation is 10-20 mg / L, more preferably 10 mg / L or 20 mg / L.

[0030] Preferably, the spraying amount of the banana plant regulator preparation is 15-25 mL / plant, more preferably 25 mL / plant; the banana plant regulator preparation is applied once every 10 days for 6 consecutive months.

[0031] Preferably, the bananas are planted at a rate of 100 to 200 plants per acre, more preferably 150 plants per acre.

[0032] Preferably, the banana plant growth regulator composition preparation is used for banana tissue culture seedlings; the length of the banana tissue culture seedlings is 18 cm to 22 cm; the number of leaves is 8 to 10; the pseudostem thickness of the banana tissue culture seedlings is ≥ 1 cm.

[0033] The banana plant growth regulator composition preparation of the present invention can be prepared into milk powder, granules or solutions according to different types of auxiliary agents.

[0034] Preferably, the preparation is a solution; the auxiliary agent includes one or more of water, methanol, ethanol, glycerol, n-propanol, isopropanol, acetone, cyclopentanone, cyclohexanone or ethyl acetate.

[0035] The present invention also provides a planting method for promoting the growth of banana plants, comprising the following steps:

[0036] 1) Planting: Choose suitable land and plant banana plants;

[0037] 2) Applying foliar fertilizer: using the banana plant regulator composition or the banana plant regulator preparation as foliar fertilizer for banana plants.

[0038] Preferably, the banana plant comprises a banana tissue culture seedling; the banana tissue culture seedling has a length of 18 cm to 22 cm and 8 to 10 leaves; and the pseudostem of the banana tissue culture seedling is ≥1 cm thick.

[0039] Preferably, the application amount of the banana plant growth regulator preparation is 15-25 mL / plant, more preferably 25 mL / plant.

[0040] Preferably, the banana plant growth regulator preparation is applied once every 10 to 15 days for 4 to 6 months; more preferably, the banana plant growth regulator preparation is applied once every 10 days for 6 months.

[0041] Preferably, foliar fertilizer is applied one week after the banana plants are transplanted; the foliar fertilizer is preferably applied by spraying.

[0042] Preferably, the bananas are planted at a rate of 100 to 200 plants per acre, more preferably 150 plants per acre.

[0043] Preferably, the planting method for promoting the growth of banana plants further comprises: before planting, deep plowing of the soil, weeding, pest control and disease prevention of the soil, and application of basal fertilizer; the soil is a plot with a deep soil layer, good drainage, a pH value of 7.0, and the soil is deep plowed to 65 cm; the basal fertilizer application is specifically: 6 kg of peanut bran, 2 kg of sugarcane bagasse, 5 kg of corn cobs, 25 kg of decomposed pig manure and 2 kg of rice husks per mu.

[0044] Preferably, the planting method for promoting the growth of banana plants further comprises applying root fertilizer: from January to February after the banana seedlings are planted, a fertilizer solution prepared from decomposed manure and water is applied to the roots, and 50 kg of human and animal manure is applied per mu; from March to June after the banana seedlings are planted, the following fertilizers are applied per mu: 20 kg of human and animal manure, 15 kg of potassium chloride, 10 kg of urea, 12 kg of superphosphate and 25 kg of diammonium phosphate, which are then evenly mixed and evenly spread in the trench dug below the roots of the plants, and fertilizer is applied once every 20 days.

[0045] Preferably, the planting method for promoting the growth of banana plants also includes preventing and controlling pests and diseases: spraying pesticides once every 20 days, digging out plants infected with bunchy top disease, and replanting them next to the holes.

[0046] The present invention creatively combines gibberellins, naphthoxyacetic acid, 14-hydroxy brassinolide and arabinose and fixes the ratio thereof, and through the synergistic effect of each component, significantly improves the stem height, stem diameter and chlorophyll content of banana plants, promotes the growth of banana plants, and has broad development and application prospects. The experimental examples of the present invention have confirmed that the composition is applied as a foliar fertilizer, and the stem height of banana tissue culture seedlings is increased by ≥48.7%, the stem diameter is increased by ≥71.6%, and the chlorophyll content is increased by ≥58.5% compared with the control group after 6 months of planting. DETAILED DESCRIPTION

[0047] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] In order to further illustrate the present invention, the following examples are used to explain it in detail. The raw materials used in the following examples and comparative examples of the present invention are all commercially available products, and the sources of the raw materials are shown in Table 1.

[0049] Table 1

[0050]

[0051] Examples 1-3

[0052] The components and contents of the banana plant growth regulator compositions of Examples 1-3 are shown in Table 2.

[0053] Table 2 Components and contents of banana plant conditioning composition

[0054]

[0055]

[0056] The preparation method of the banana plant regulating agent composition comprises: mixing the components in Table 2 according to corresponding contents, stirring evenly, and obtaining the banana plant regulating agent composition.

[0057] Comparative Example 1

[0058] The banana plant growth regulator composition of this comparative example is different from that of Example 1 in that this comparative example only includes gibberellins, 2-naphthoxyacetic acid and 14-hydroxybrassinosteroid, and the missing amount of DL-arabinose is distributed to the corresponding components according to the ratio of the other three substances in Example 1.

[0059] Comparative Example 2

[0060] The banana plant growth regulator composition of this comparative example is different from that of Example 1 in that gibberellin is replaced by zeatin of the same mass in this comparative example; the remaining components and contents are the same as those of Example 1.

[0061] Comparative Example 3

[0062] The banana plant growth regulator composition of this comparative example is different from that of Example 1 in that 2-naphthoxyacetic acid is replaced by 1-naphthylacetic acid of the same mass in this comparative example; the remaining components and contents are the same as those of Example 1.

[0063] Comparative Example 4

[0064] The banana plant growth regulator composition of this comparative example is different from that of Example 1 in that 14-hydroxybrassinosteroid is replaced by brassinolide of the same mass in this comparative example; the remaining components and contents are the same as those of Example 1.

[0065] Comparative Examples 5-8

[0066] The banana plant regulators of Comparative Examples 5-8 are single substances of gibberellin, 2-naphthoxyacetic acid, 14-hydroxybrassinosteroid and DL-arabinose, respectively, and their contents are the same as the total amount of the banana plant regulator composition in Example 1. For example, Comparative Example 5 is 19g of gibberellin.

[0067] Application Examples 1-3

[0068] The banana plant growth regulator compositions of Examples 1-3 are mixed with a solvent and water respectively to prepare a banana plant growth regulator preparation. The specific method comprises the following steps:

[0069] The banana plant regulator compositions of Examples 1-3 were respectively dissolved in a solvent consisting of 5 g ethanol + 5 g glycerol, and diluted with water to obtain a banana plant regulator preparation, wherein the concentration of the banana plant regulator composition was 10 mg / L.

[0070] Application Examples 4-6

[0071] The banana plant growth regulator compositions of Examples 1-3 are mixed with a solvent and water respectively to prepare a banana plant growth regulator preparation. The specific method comprises the following steps:

[0072] The banana plant regulator compositions of Examples 1-3 were respectively dissolved in a solvent consisting of 5 g ethanol + 5 g glycerol, and diluted with water to obtain a banana plant regulator preparation, wherein the concentration of the banana plant regulator composition was 20 mg / L.

[0073] Application Comparative Examples 1-8

[0074] The banana plant regulators of Comparative Examples 1-8 are respectively mixed with a solvent and water to prepare a banana plant regulator preparation. The specific method comprises the following steps:

[0075] The banana plant regulators of Comparative Examples 1-8 were respectively dissolved in a solvent consisting of 5g ethanol + 5g glycerol, and diluted with water to obtain a banana plant regulator preparation, wherein the concentration of the banana plant regulator composition was 10 mg / L.

[0076] Experimental example

[0077] 1) Preparation

[0078] 1.1 Select a plot with deep soil, good drainage and pH value of 7.0, turn the soil 65 cm deep, remove weeds at the same time, and then dilute 50% phoxim emulsifiable concentrate 800 times and spray it on the surface of the banana field to prevent diseases and pests.

[0079] 1.2 Apply base fertilizer: 6kg of peanut bran, 2kg of sugarcane bagasse, 5kg of corn cobs, 25kg of decomposed pig manure and 2kg of rice husk per mu.

[0080] 2) Planting of banana seedlings

[0081] Select Brazilian banana (Mimosa nana Lour.) tissue culture seedlings with uniform growth, 18cm to 22cm in length, 8 to 10 leaves, and a stiff pseudostem with a thickness of ≥1cm; plant the banana seedlings at a row spacing of 3m and a row spacing of 2m, plant 150 seedlings per mu of land, and then cover the pits with soil.

[0082] 3) Field management

[0083] 3.1 Foliar fertilizer application: One week after the banana seedlings were transplanted, an experimental group and a control group were set up. The experimental group was the banana planting agent preparation of application examples 1-6 and application comparison examples 1-8; the control group was water; the components of the experimental group and the control group were respectively sprayed on the leaves of the banana seedlings, with a spraying amount of 25 mL / plant, once every 10 days, for 6 consecutive months.

[0084] 3.2 Apply root fertilizer: From January to February after the banana seedlings are planted, the fertilizer solution made of rotted manure and water is applied to the roots, and 50 kg of human and animal manure is applied per mu; from March to June after the banana seedlings are planted, the following fertilizers are applied per mu: 20 kg of human and animal manure, 15 kg of potassium chloride, 10 kg of urea, 12 kg of superphosphate and 25 kg of diammonium phosphate, then mix them evenly, and then evenly spread them in the trench dug under the roots of the plants, and apply fertilizer once every 20 days.

[0085] 4) Prevention and control of pests and diseases

[0086] Spray insecticide once every 20 days. If you find plants infected with bunchy top disease, dig them out and replant them near the holes.

[0087] 5) After 6 months, the following agronomic traits of all Brazilian bananas were measured. The test results are shown in Table 3.

[0088] Stem height: refers to the vertical distance from the intersection of the petioles of the two most recently grown leaves to the substrate surface;

[0089] Stem diameter: Use a vernier caliper to measure the diameter at the middle of the stem;

[0090] Chlorophyll content: The chlorophyll content in the middle of the second unfolded leaf was measured using a Top TYSB chlorophyll meter.

[0091] Table 3

[0092]

[0093] As can be seen from Table 3, the stem height, stem diameter and chlorophyll content of the banana plants treated with the plant growth regulator preparation of the application example of the present invention are significantly higher than those of the control group and the plants treated with the comparative example, indicating that the specific ratio of gibberellin, naphthoxyacetic acid, 14-hydroxybrassinosteroid and arabinose composition can significantly increase the stem height, stem diameter and chlorophyll content of banana tissue culture seedlings, and promote the growth of banana tissue culture seedlings.

[0094] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A banana planting agent composition, characterized in that: Calculated by weight, the composition comprises: 6 to 10 parts of gibberellins, 1 to 3 parts of naphthoxyacetic acid, 2 to 4 parts of 14-hydroxybrassinosteroids and 0.6 to 2 parts of arabinose.

2. The banana plant conditioning agent composition according to claim 1, characterized in that: The purity of the gibberellin is ≥90%; the naphthoxyacetic acid is 2-naphthoxyacetic acid; the purity of the naphthoxyacetic acid is ≥98%; the purity of the 14-hydroxybrassinosteroid is ≥98%; the arabinose is DL-arabinose; the purity of the arabinose is ≥98%.

3. The banana plant conditioning agent composition according to claim 1, characterized in that: In parts by mass, including: 10 parts of gibberellins, 3 parts of naphthoxyacetic acid, 4 parts of 14-hydroxybrassinosteroids and 2 parts of arabinose; Alternatively, the banana planting agent composition comprises: 6 parts of gibberellins, 2 parts of naphthoxyacetic acid, 2 parts of 14-hydroxybrassinosteroids and 0.8 parts of arabinose; Alternatively, the banana planting agent composition comprises: 7 parts of gibberellins, 1 part of naphthoxyacetic acid, 2 parts of 14-hydroxybrassinosteroids and 0.6 parts of arabinose.

4. The method for preparing the banana plant conditioning agent composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: Components including 5-15 parts of gibberellins, 1-5 parts of naphthoxyacetic acid, 2-5 parts of 14-hydroxybrassinosteroids and 0.1-2 parts of arabinose are mixed in parts by mass to obtain a banana plant growth regulator composition.

5. Use of the banana plant growth regulator composition according to any one of claims 1 to 3 in promoting banana growth.

6. A banana planting agent, characterized in that: The invention comprises the banana plant regulating agent composition and auxiliary agents as described in any one of claims 1 to 3.

7. The banana planting agent according to claim 6, characterized in that: The concentration of the banana plant growth regulator composition in the banana plant growth regulator preparation is 10-20 mg / L.

8. A planting method for promoting the growth of banana plants, characterized in that: The following steps are involved: 1) Planting: Choose suitable land and plant banana plants; 2) Applying foliar fertilizer: using the banana plant regulator composition according to any one of claims 1 to 3 or the banana plant regulator preparation according to claim 6 or 7 as foliar fertilizer for banana plants.

9. The method for promoting banana plant growth according to claim 8, characterized in that: The banana plants include banana tissue culture seedlings; The length of the banana tissue culture seedling is 18 cm to 22 cm, and the number of leaves is 8 to 10; The application amount of the banana plant regulator preparation is 15-25 mL / plant; The banana planting regulator preparation is applied once every 10 to 15 days and continuously applied for 4 to 6 months.

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