Disorienting agent for interfering with mating of cornus fruit borer adults and application thereof

Abstract

The application provides a kind of disorienting agent for interfering with mating of Cornus fruit borer adult and application, the disorienting agent includes (Z)-7-eicosen-11-one one monomer compound or (Z)-7-eicosen-11-one, (Z)-7-tricosene two compound mixtures;In the mixture, (Z)-7-eicosen-11-one and (Z)-7-tricosene are used in a ratio of 2:1.The disorienting agent is applied, and needs to be prepared as disorienting silk and arranged in the field.The efficient and stable sex pheromone disorienting agent for interfering with mating of Cornus fruit borer adult provided by the application is strong in specificity, safe and environmentally friendly, easy to operate, efficient and economical, has a long effective period, can be used for large-area promotion and application in the field, reduces the use of chemical pesticides in the field, improves the safety of Cornus officinalis medicinal materials, and maintains the stability of ecological environment.

Description

Technical Field

[0001] This invention relates to the field of biological control technology, specifically to a mating determinant for interfering with the mating of adult Cornus officinalis moths and its application. Background Technology

[0002] Cornus officinalis Cornus officinalis Sieb. et Zucc. belongs to the Cornaceae family and the Cornus genus. Horn It is a deciduous shrub or small tree, and its dried, ripe fruit pulp is used medicinally. It has the effects of tonifying the liver and kidneys and stopping sweating, and is a commonly used traditional Chinese medicine. In my country, it is mainly produced in several provinces such as Henan, Shaanxi, Shanxi, Zhejiang, and Anhui, and is basically in a semi-wild state.

[0003] Cornus officinalis fruit borer Korean carposin The Cornus fruit borer (Lepidoptera: Carposinidae) is currently the most serious pest affecting Cornus officinalis cultivation areas. It is widely distributed in major Cornus officinalis producing areas in my country, particularly in Henan, Shaanxi, and Shanxi provinces. The larvae bore into the fruit, feeding on the pulp by creating a network of tunnels until only the outer layer of skin remains. The hollowed-out fruit is filled with frass and loses its medicinal value. In severe infestations, over 90% of Cornus officinalis trees and 30-40% of fruits are affected, with some cases exceeding 90%, causing significant losses to the Cornus officinalis industry.

[0004] Currently, the control of the Cornus fruit borer mainly relies on spraying chemical pesticides on the tree canopy. However, because this insect is a borer, once the larvae have bored into the fruit, even pesticide application is not very effective. Furthermore, Cornus fruits mostly grow in mountainous forests where the trees are tall, making pesticide application extremely difficult. Indiscriminate and frequent pesticide use leads to problems such as accelerated insect resistance, decreased quality of Cornus medicinal materials, and excessive pesticide residues, causing the occurrence and damage of the Cornus fruit borer to continue to spread year by year, directly affecting the quality of Cornus medicinal materials and the health of pesticide users. Therefore, to date, there is no successful method for controlling the Cornus fruit borer.

[0005] The insect sex pheromone maze technique, also known as mating interference, involves releasing high concentrations of artificially synthesized sex pheromones in the field. This causes male insects to lose their sense of smell, making them unable to identify and orient themselves towards females, thus disrupting mating. Alternatively, prolonged exposure of the males' antennae to the high concentration of sex pheromones paralyzes them, rendering them unable to respond to the females' calls. Without mating, the females cannot reproduce and cause damage, ultimately achieving pest control. Compared to conventional integrated pest management techniques, sex pheromone maze control significantly reduces the occurrence and damage of the Cornus officinalis fruit borer, further suppressing its occurrence and population. Summary of the Invention

[0006] In order to overcome the shortcomings of the prior art, the purpose of this invention is to provide a mating agent that can interfere with the mating of adult Cornus officinalis moths and its application method.

[0007] To solve the above problems, the technical solution adopted by the present invention is as follows:

[0008] A mating determinant for interfering with the mating of adult Cornus officinalis moths, comprising a monomeric compound (Z)-7-eicosenoen-11-one or a mixture of two compounds, (Z)-7-eicosenoen-11-one and (Z)-7-tetracarne; wherein the mixture is used in a ratio of (Z)-7-eicosenoen-11-one to (Z)-7-tetracarne in a ratio of 2:1.

[0009] The deflector also includes 1%-2% of a UV stabilizer and 1%-2% of an antioxidant by mass.

[0010] A mating silk for interfering with the mating of adult Cornus officinalis fruit borers includes a mating agent and a carrier; the mating agent is attached to the carrier.

[0011] The carrier is a capillary made of materials such as hollow polyethylene or polyvinyl chloride, with an outer diameter of 0.5~1.5mm, an inner diameter of 50~500mm, and a length of 20~50cm.

[0012] When the machinator contains a single component (Z)-7-eicosene-11-one: each machinator filament contains 40-200 mg of machinator, and 30-50 machinators are set per acre, the machination rate can reach more than 80%; more preferably, each machinator filament contains 100-200 mg of machinator, and 40-50 machinators are set per acre, the machination rate can reach more than 90%.

[0013] When the machinator contains two compounds, (Z)-7-eicosene-11-one and (Z)-7-tetracarne: each machinator contains 60-300 mg of machinator, and 20-50 machinators are set per acre, the machination rate can reach more than 80%; more preferably, when each machinator contains 90-300 mg of machinator, and 30-50 machinators are set per acre, the machination rate can reach more than 90%.

[0014] The method for preparing the maze includes:

[0015] The demagnetizing agent is dissolved in an organic solvent to prepare a demagnetizing agent solution;

[0016] The misdirector solution, antioxidant, and UV stabilizer are injected into the misdirector filament carrier in a certain proportion, and the two ends are sealed to obtain the product.

[0017] A method for field application of maze silk includes: during the emergence period of adult dogwood fruit borers, the maze silk is wrapped and fixed to the branches on the outer periphery of the dogwood tree crown at a height of about 1.5-2m above the ground.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] The present invention provides a highly efficient and stable sex pheromone mating agent that specifically interferes with the mating of adult Cornus officinalis fruit borers. It is highly specific, safe and environmentally friendly, easy to operate, economical and efficient, and has a long-lasting effect. It can be widely promoted and applied in the field, reducing the use of chemical pesticides in the field, improving the safety of using Cornus officinalis medicinal materials, and maintaining the stability of the ecological environment.

[0020] The present invention will be further described in detail below with reference to specific embodiments. Detailed Implementation

[0021] This embodiment provides a mating agent for interfering with the mating of adult Cornus officinalis moths, comprising sex pheromones, UV stabilizers, and antioxidants. The sex pheromones comprise either a monomeric compound (Z)-7-eicosenoen-11-one or a mixture of (Z)-7-eicosenoen-11-one and (Z)-7-tetracarne; in the mixture, the ratio of (Z)-7-eicosenoen-11-one to (Z)-7-tetracarne is 2:1. In the mating agent, the sex pheromones constitute 96%-98% by mass, while the UV stabilizers and antioxidants each constitute 1%-2%. In this embodiment, the sex pheromones constitute 96% by mass, while the UV stabilizers and antioxidants each constitute 2%. The UV stabilizer includes at least one of UV-531, UV-328, and UV-P, and UV-531 is used in this embodiment; the antioxidant is selected from at least one of vitamin E, tert-butylhydroquinone, 2,6'-di-tert-butyl-4-methylphenol, and butylated hydroxyanisole, and vitamin E is used in this embodiment.

[0022] Table 1. Content of neutral pheromones, antioxidants, and UV stabilizers in the attractant.

[0023]

[0024] Note: Component A is (Z)-7-eicosenoen-11-one, and component B is (Z)-7-tetracarne.

[0025] In practical applications, the attractant needs to be processed into attractant threads. The preparation of attractant threads requires the aforementioned attractant and attractant carrier; the attractant is attached to the attractant thread carrier. The specific preparation method of the attractant threads is as follows: the attractant is dissolved in an organic solvent to prepare an attractant solution; then, the attractant solution, antioxidant, and UV stabilizer are injected into the attractant thread carrier in a specific ratio, and both ends are sealed to obtain the sex pheromone attractant thread of the Cornus officinalis moth. In this embodiment, petroleum ether is used as the organic solvent.

[0026] In this embodiment, the maze carrier is a capillary made of materials such as hollow polyethylene or polyvinyl chloride, with an inner diameter of 0.5 mm, a wall thickness of 0.05 mm, and a maze length of 30 cm.

[0027] During the adult emergence period of the Cornus officinalis fruit borer (July to September), 18 plots with similar planting environments, conditions, tree ages, and areas were selected from the Cornus officinalis planting base. Each plot was spaced approximately 200 meters apart to prevent mutual interference between plots. Each experimental plot covered approximately 10 mu (about 0.67 hectares). No chemical pesticides were applied to any plots during the experiment. Specifically, treatment plots 1-9 were mating test areas using different doses of a single-component sex pheromone (Z)-7-eicosenoen-11-one), and plot 10 was a control test area using a single-component sex pheromone lure. Treatment plots 11-19 were mating test areas using different doses of two-component sex pheromones A+B (A being (Z)-7-eicosenoen-11-one and B being (Z)-7-tetracarne), and plot 20 was a control test area using a two-component sex pheromone lure.

[0028] The prepared mating silk was directly wrapped around the branches on the outer edge of the Cornus officinalis canopy, according to the quantities shown in Tables 1 and 2, ensuring even coverage throughout the experimental area to eliminate differences caused by uneven release. Three sets of Cornus officinalis fruit borer sex pheromone traps were set up in each treatment plot for monitoring, using rubber-tipped lures and triangular traps as the carriers and trapping devices for the sex pheromone attractant. Fewer adult Cornus officinalis fruit borers were trapped, indicating a better mating effect, and vice versa.

[0029] After placing the mating silk and traps, the number of male dogwood fruit borers trapped on the sticky boards in each trap was counted every 3 days. After each count, the positions of the traps in each treatment area were randomly changed to eliminate differences in the number of traps caused by environmental factors, and the sticky boards were replaced in a timely manner.

[0030] The mating rate was used as an evaluation index for sex pheromone mating agents. A higher mating rate indicated a better control effect of the mating agent against the dogwood fruit borer moth, and vice versa. The formula for calculating the mating rate is as follows:

[0031] Mating rate (%) = (Number of moths attracted by traps in the control area - Number of moths attracted by traps in the mating agent-treated area) / Number of moths attracted by traps in the control area * 100%

[0032] The specific data of the test results are shown in Tables 2 and 3:

[0033] Table 2. Trapping effect and forest mating rate of different doses of single component sex pheromones on mating silk (A)

[0034]

[0035] Note: Component A is (Z)-7-eicosene-11-one.

[0036] Table 3. Trapping effect and forest mating rate of different doses of the two components of sex pheromones (A+B)

[0037]

[0038] Note: Component A is (Z)-7-eicosenoen-11-one, and component B is (Z)-7-tetracarne.

[0039] See Table 1 for the results of the single-component maze test:

[0040] When each mating thread contains 40-200 mg of mating agent, and 30-50 mating threads are planted per acre, the mating rate can reach over 80%. More preferably, when each mating thread contains 100-200 mg of mating agent, and 40-50 mating threads are planted per acre, the mating rate can reach over 90%.

[0041] See Table 2 for the results of the deflection test on the two-component mixture:

[0042] When each mating thread contains 60-300 mg of mating agent, and 20-50 mating threads are planted per acre, the mating rate can reach over 80%. More preferably, when each mating thread contains 90-300 mg of mating agent, and 30-50 mating threads are planted per acre, the mating rate can reach over 90%.

[0043] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A tropil, for interfering with mating of Cornus fruit borer adults, characterized in that, It includes a monomeric compound (Z)-7-eicosenoen-11-one or a mixture of two compounds, (Z)-7-eicosenoen-11-one and (Z)-7-tetratene; in the mixture, the ratio of (Z)-7-eicosenoen-11-one to (Z)-7-tetratene is 2:1; when the machinator contains a single component (Z)-7-eicosenoen-11-one, each machinator wire contains 40-200 mg of machinator, and 30-50 machinator wires are set per acre; when the machinator contains both (Z)-7-eicosenoen-11-one and (Z)-7-tetratene, each machinator wire contains 60-300 mg of machinator, and 20-50 machinator wires are set per acre.

2. The geotropic agent according to claim 1, characterized in that, The deflector also includes 1%-2% of a UV stabilizer and 1%-2% of an antioxidant by mass.

3. A disorientating thread for interfering with mating of Cornus fruit borer adults, characterized in that, It includes the deflecting agent and carrier as described in claim 1 or 2; the deflecting agent is attached to the carrier.

4. The maze wire of claim 3, wherein, Each mating wire contains 100-200 mg of mating agent, and 40-50 mating wires are planted per acre.

5. The maze wire of claim 3, wherein, Each mating wire contains 90-300mg of mating agent, and 30-50 mating wires are planted per acre.

6. A method of producing the aposome of any one of claims 3 to 5, characterized in that, include: The demagnetizing agent is dissolved in an organic solvent to prepare a demagnetizing agent solution; The misdirector solution, antioxidant, and UV stabilizer are injected into the misdirector filament carrier in a certain proportion, and the two ends are sealed to obtain the product.

7. A method of field use of the disorienting thread according to any one of claims 3 to 5, characterized in that, include: During the emergence period of the Cornus fruit borer moth, the maze silk is wrapped and fixed to the branches on the outer edge of the Cornus tree crown, at a height of 1.5-2m above the ground.

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