A toothpaste containing probiotics for preventing moths and inhibiting bacteria and a preparation method thereof

Abstract

The invention discloses a toothpaste containing probiotics for preventing caries and inhibiting bacteria. The toothpaste comprises the following components by weight: 1-5 parts of probiotic freeze-dried powder, 3-8 parts of carboxymethyl chitosan-manganese gluconate sol, 1-5 parts of naringin, 0.5-3 parts of fisetin, 20-45 parts of sorbitol, 10-25 parts of cocamidopropyl betaine, 1-5 parts of cellulose gum and 50-70 parts of deionized water. The multiple beneficial ingredients synergistically inhibit the growth of pathogenic bacteria to form a barrier to prevent the colonization and infection of pathogenic bacteria. The manganese gluconate not only has good antibacterial and antioxidant properties, but also can provide manganese ions. The manganese ions are combined with hydroxyapatite on the surface of teeth to form a protective layer similar to natural teeth, which is helpful to promote the remineralization process of teeth, thereby reducing the occurrence of tooth decay on the tooth surface.

Description

Technical Field

[0001] The invention relates to the technical field of oral care, and in particular to a toothpaste containing probiotics for preventing caries and inhibiting bacteria, and a preparation method thereof. Background Art

[0002] Oral health is one of the top ten criteria for overall health. As one of the key points for the prevention and treatment of chronic diseases, oral diseases have been listed by the WHO as the third largest disease after cancer and cerebrovascular diseases. The latest oral epidemiological survey report shows that the prevalence of oral diseases such as tooth decay and periodontal disease in my country is as high as 97.6%. Studies have shown that dental health can prevent a variety of oral diseases, such as caries and periodontitis. Dental health is also closely related to overall health. Oral diseases may increase the risk of cardiovascular diseases, diabetes and other systemic diseases, and maintaining dental health can help reduce these risks.

[0003] Anti-caries and antibacterial toothpaste is a toothpaste designed for oral health problems. Its main function is to prevent caries and inhibit the growth of oral bacteria. This type of toothpaste contains some antibacterial and anti-caries ingredients to help keep the mouth clean and healthy. Traditional anti-caries and antibacterial toothpaste mainly uses fluoride and some conventional antibacterial agents as the main ingredients. Its antibacterial spectrum is limited and can only target some oral bacteria. It may not be effective against some drug-resistant bacteria or specific strains. Some antibacterial ingredients last for a short time in the mouth and are quickly washed away or become ineffective after brushing, causing oral bacteria to grow again and making it difficult to keep the mouth clean for a long time. With the continuous advancement of science and technology, researchers are looking for more effective antibacterial ingredients to replace or enhance traditional ingredients such as fluoride and sodium trifluorosilicate.

[0004] Therefore, there is an urgent need to develop a new type of toothpaste for preventing caries and inhibiting bacteria, so as to improve the effect and quality of the toothpaste, and thus better meet people's needs for oral health. Summary of the invention

[0005] In view of this, the present invention provides a toothpaste containing probiotics for preventing caries and inhibiting bacteria to solve the above problems.

[0006] The technical solution of the present invention is achieved in this way:

[0007] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 1-5 parts of probiotic freeze-dried powder, 3-8 parts of carboxymethyl chitosan-manganese gluconate sol, 1-5 parts of naringin, 0.5-3 parts of fisetin, 20-45 parts of sorbitol, 10-25 parts of cocamidopropyl betaine, 1-5 parts of cellulose gum and 50-70 parts of deionized water.

[0008] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 3 parts of probiotic freeze-dried powder, 5 parts of carboxymethyl chitosan-manganese gluconate sol, 3 parts of naringin, 1.5 parts of fisetin, 30 parts of sorbitol, 20 parts of cocamidopropyl betaine, 3 parts of cellulose gum and 60 parts of deionized water.

[0009] Preferably, the mass ratio of carboxymethyl chitosan to manganese gluconate in the carboxymethyl chitosan-manganese gluconate sol is 3-5:1.

[0010] The probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; the number of live bacteria of the Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g.

[0011] The present invention also provides a method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria, comprising the following steps:

[0012] S1, preparation of carboxymethyl chitosan-manganese gluconate sol;

[0013] S2, weighing naringin, fisetin, and carboxymethyl chitosan-manganese gluconate sol, and mixing them to obtain a mixture A;

[0014] S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer, heating and stirring, to obtain a mixture B;

[0015] S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 15 to 20 minutes;

[0016] S5. Perform vacuum degassing to obtain toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0017] Preferably, the preparation method of the S1 carboxymethyl chitosan-manganese gluconate sol is: prepare an acetic acid-sodium acetate buffer with a pH value of 4-6, disperse the carboxymethyl chitosan in the acetic acid-sodium acetate buffer to obtain a suspension, add manganese gluconate to the suspension, stir, filter, wash, and dry to obtain the carboxymethyl chitosan-manganese gluconate sol.

[0018] Preferably, the heating temperature in S3 is 60-70° C.; the stirring speed is 1000-2000 r / min, and the stirring time is 25-40 min.

[0019] Preferably, the vacuum degree of the S5 vacuum degassing is 0.2-0.4 MPa; and the degassing time is 50-60 min.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The present invention provides a toothpaste containing probiotics for preventing caries and inhibiting bacteria. The antibacterial substances are reasonably proportioned with probiotic freeze-dried powder, carboxymethyl deacetylated chitosan-manganese gluconate sol, naringin, and fisetin components. The multiple beneficial ingredients synergistically inhibit the growth of pathogenic bacteria, forming a barrier to prevent pathogenic bacteria from colonizing and infecting.

[0022] (2) Manganese gluconate not only has good antibacterial and antioxidant properties, but also can provide manganese ions. Manganese ions combine with hydroxyapatite on the surface of teeth to form a protective layer similar to natural teeth, which helps promote the remineralization process of teeth and thus reduce the occurrence of tooth decay on the tooth surface.

[0023] (3) Carboxymethyl chitosan-manganese chloride in the form of a sol can more easily achieve uniform distribution of ingredients, has better solubility, can dissolve faster in the oral cavity, and release manganese chloride ions; carboxymethyl chitosan-manganese chloride in the form of a sol has better stability, can maintain its effectiveness in toothpaste for a longer period of time, is not prone to decomposition or failure, thereby improving the care effect of the toothpaste. DETAILED DESCRIPTION

[0024] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0025] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0026] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0027] Bifidobacterium lactis was purchased from Shanghai Microbiological Collection Center with the collection number SHBCCD24675.

[0028] Naringin, INCI English name Naringin, CAS No. 10236-47-2, was purchased from Guangdong Hongyou Biotechnology Co., Ltd.

[0029] Fisetin, INCI English name Fisetin, CAS No. 528-48-3, was purchased from Nanjing Dosfu Biotechnology Co., Ltd.

[0030] Example 1

[0031] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 1 part of probiotic freeze-dried powder, 3 parts of carboxymethyl chitosan-manganese gluconate sol, 1 part of naringin, 0.5 part of fisetin, 20 parts of sorbitol, 10 parts of cocamidopropyl betaine, 1 part of cellulose gum, and 50 parts of deionized water; the probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; and the number of live bacteria of the Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g.

[0032] A method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following steps:

[0033] S1. Preparation of carboxymethyl chitosan-manganese gluconate sol: prepare an acetic acid-sodium acetate buffer with a pH of 4.5, disperse carboxymethyl chitosan in 0.5 mol / L acetic acid-sodium acetate buffer, wherein the amount of the buffer added is 6 times the mass of the carboxymethyl chitosan, to obtain a suspension, add manganese gluconate with a mass of 1 / 3 of the mass of the carboxymethyl chitosan to the suspension and stir; stir at 30° C. for 12 h, filter, wash, and dry to obtain carboxymethyl chitosan-manganese gluconate sol;

[0034] S2, weighing naringin, fisetin, and carboxymethyl chitosan-manganese gluconate sol, and mixing them to obtain a mixture A;

[0035] S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum and deionized water, placing them in a stirrer and heating and stirring, the heating temperature is 60° C., the stirring speed is 1000 r / min, and the stirring time is 25 min, to obtain a mixture B;

[0036] S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 15 minutes;

[0037] S5. Perform vacuum degassing, wherein the vacuum degree of the vacuum degassing is 0.2 MPa and the degassing time is 50 min, to obtain a toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0038] Example 2

[0039] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 3 parts of probiotic freeze-dried powder, 5 parts of carboxymethyl chitosan-manganese gluconate sol, 3 parts of naringin, 1.5 parts of fisetin, 30 parts of sorbitol, 20 parts of cocamidopropyl betaine, 3 parts of cellulose gum, and 60 parts of deionized water; the probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; and the number of live bacteria of the Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g.

[0040] A method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following steps:

[0041] S1. Preparation of carboxymethyl chitosan-manganese gluconate sol: prepare an acetic acid-sodium acetate buffer with a pH of 5.5, disperse carboxymethyl chitosan in 0.5 mol / L acetic acid-sodium acetate buffer, wherein the amount of the buffer added is 6 times the mass of the carboxymethyl chitosan, to obtain a suspension, add manganese gluconate with a mass of 1 / 4 of the mass of the carboxymethyl chitosan to the suspension and stir; stir at 30° C. for 12 h, filter, wash, and dry to obtain carboxymethyl chitosan-manganese gluconate sol;

[0042] S2, weighing naringin, fisetin, and carboxymethyl chitosan-manganese gluconate sol, and mixing them to obtain a mixture A;

[0043] S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer and heating and stirring, the heating temperature is 65° C., the stirring speed is 1500 r / min, and the stirring time is 35 min, to obtain a mixture B;

[0044] S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 20 minutes;

[0045] S5. Perform vacuum degassing, wherein the vacuum degree of the vacuum degassing is 0.3 MPa and the degassing time is 60 min, to obtain a toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0046] Example 3

[0047] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 5 parts of probiotic freeze-dried powder, 8 parts of carboxymethyl chitosan-manganese gluconate sol, 5 parts of naringin, 3 parts of fisetin, 45 parts of sorbitol, 25 parts of cocamidopropyl betaine, 5 parts of cellulose gum, and 70 parts of deionized water; the probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; the number of live bacteria of the Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g.

[0048] A method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following steps:

[0049] S1. Preparation of carboxymethyl chitosan-manganese gluconate sol: prepare an acetic acid-sodium acetate buffer with a pH of 6, disperse carboxymethyl chitosan in 0.5 mol / L acetic acid-sodium acetate buffer, wherein the amount of the buffer added is 6 times the mass of the carboxymethyl chitosan, to obtain a suspension, add manganese gluconate with a mass of 1 / 5 of the mass of the carboxymethyl chitosan to the suspension and stir; stir at 30° C. for 12 h, filter, wash, and dry to obtain carboxymethyl chitosan-manganese gluconate sol;

[0050] S2, weighing naringin, fisetin, and carboxymethyl chitosan-manganese gluconate sol, and mixing them to obtain a mixture A;

[0051] S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer and heating and stirring, the heating temperature is 70° C., the stirring speed is 2000 r / min, and the stirring time is 40 min, to obtain a mixture B;

[0052] S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 20 minutes;

[0053] S5. Perform vacuum degassing, wherein the vacuum degree of the vacuum degassing is 0.4 MPa and the degassing time is 60 min, to obtain a toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0054] Comparative Example 1

[0055] This example provides a toothpaste containing probiotics for anti-caries and antibacterial purposes. Its components and preparation method are basically the same as those in Example 2, except that manganese gluconate is not added to the carboxymethyl deacetylated chitosan-manganese gluconate sol in this example, which is a single carboxymethyl deacetylated chitosan sol.

[0056] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 3 parts of probiotic freeze-dried powder, 5 parts of carboxymethyl deacetylated chitosan sol, 3 parts of naringin, 1.5 parts of fisetin, 30 parts of sorbitol, 20 parts of cocamidopropyl betaine, 3 parts of cellulose gum, and 60 parts of deionized water; the probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; the number of live bacteria of the Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g.

[0057] A method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following steps:

[0058] S1. Preparation of carboxymethyl chitosan sol: prepare an acetic acid-sodium acetate buffer with a pH of 5.5, disperse carboxymethyl chitosan in 0.5 mol / L acetic acid-sodium acetate buffer, wherein the amount of the buffer added is 6 times the mass of the carboxymethyl chitosan, to obtain a suspension, stir at 30° C. for 12 h, filter, wash, and dry to obtain a carboxymethyl chitosan sol;

[0059] S2, weighing naringin, fisetin, and carboxymethyl chitosan sol, and mixing them to obtain a mixture A;

[0060] S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer and heating and stirring, the heating temperature is 65° C., the stirring speed is 1500 r / min, and the stirring time is 35 min, to obtain a mixture B;

[0061] S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 20 minutes;

[0062] S5. Perform vacuum degassing, wherein the vacuum degree of the vacuum degassing is 0.3 MPa and the degassing time is 60 min, to obtain a toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0063] Comparative Example 2

[0064] This example provides a toothpaste containing probiotics for preventing caries and inhibiting bacteria. Its components and preparation method are basically the same as those in Example 2, except that in this example, carboxymethyl deacetylated chitosan and manganese gluconate are not directly added in the form of sol.

[0065] A toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following components by weight: 3 parts of probiotic freeze-dried powder, 4 parts of carboxymethyl chitosan, 1 part of manganese gluconate, 3 parts of naringin, 1.5 parts of fisetin, 30 parts of sorbitol, 20 parts of cocamidopropyl betaine, 3 parts of cellulose gum, and 60 parts of deionized water; the probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; the number of live bacteria of Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g; the mass ratio of carboxymethyl chitosan to manganese gluconate is 4:1.

[0066] A method for preparing a toothpaste containing probiotics for preventing caries and inhibiting bacteria comprises the following steps:

[0067] S1. Weigh carboxymethyl chitosan, manganese gluconate, naringin and fisetin, and mix them to obtain a mixture A;

[0068] S2, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer and heating and stirring, the heating temperature is 65° C., the stirring speed is 1500 r / min, and the stirring time is 35 min, to obtain a mixture B;

[0069] S3, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 20 minutes;

[0070] S4, performing vacuum degassing, wherein the vacuum degree of the vacuum degassing is 0.3 MPa, and the degassing time is 60 min, to obtain a toothpaste containing probiotics for preventing caries and inhibiting bacteria.

[0071] Comparative Example 3

[0072] This example provides a toothpaste containing probiotics for preventing caries and inhibiting bacteria. Its components and preparation method are basically the same as those in Example 2, except that naringin is not added in this example, and the amount of fisetin added is adjusted to 4.5 parts.

[0073] Comparative Example 4

[0074] This example provides a toothpaste containing probiotics for preventing caries and inhibiting bacteria. Its components and preparation method are basically the same as those in Example 2, except that in this example, fisetin is not added and the amount of naringin added is adjusted to 4.5 parts.

[0075] Test Example 1 Antibacterial Performance Detection

[0076] The antibacterial performance of the toothpastes of Examples 1-3 and Comparative Examples 1-4 was tested by the Oxford cup method (tube-plate method), which is a common method for determining the potency of antibiotics at home and abroad. The inhibition zone test was used to determine the inhibitory effect of the tested samples on Streptococcus mutans (ATCC 25175), Porphyromonas gingivalis (ATCC 33277), and Escherichia coli (ATCC 25922).

[0077] Test sample: Take 100 mg of each toothpaste prepared in Examples 1-3 and Comparative Examples 1-4, dilute with sterile deionized water at a ratio of 1:1 to prepare the test sample, and perform the following test: Pour the culture medium sterilized by high temperature into a sterile culture dish with a diameter of 9 cm using aseptic operation, so that each culture dish has 15-20 mL of culture medium, and after cooling, add 1 mL of Streptococcus mutans, Porphyromonas gingivalis, and Escherichia coli suspension (concentration of 1.0×10 7 / mL), use a coating rod to evenly coat the surface of the culture medium, 21 culture dishes for each type of bacteria (divided into 7 groups on average, 3 in each group, corresponding to Examples 1-3 and Comparative Examples 1-4, respectively), use tweezers to vertically place the Oxford cup on the surface of the corresponding culture medium, gently pressurize it so that it is in contact with the culture medium without gaps, drop 0.1mL of various samples to be tested into each small tube, mark it well, and do not let it overflow, place it in a 37°C incubator for anaerobic culture for 24 hours, and observe the results. During the culture, on the one hand, the test bacteria begin to grow, and on the other hand, the sample to be tested diffuses around with the Oxford cup as the origin, forming a circular area where the colony cannot grow, i.e., the "inhibition zone". The diameter of the inhibition zone in each group of culture dishes after 24 hours is measured and the average value is taken. The results are shown in Table 1 below.

[0078] Table 1 Antibacterial diameter of each group of toothpaste on various pathogens (mm)

[0079] Group Streptococcus mutans Porphyromonas gingivalis Escherichia coli Example 1 25.9 28.5 26.4 Example 2 27.1 29.7 27.9 Example 3 26.5 28.9 27.2 Comparative Example 1 18.9 20.1 17.4 Comparative Example 2 20.3 19.2 19.8 Comparative Example 3 17.8 18.4 15.3 Comparative Example 4 16.5 19.0 16.7

[0080] As can be seen from Table 1, the toothpastes prepared in Examples 1-3 of the present invention have good inhibitory effects on various pathogenic bacteria in the oral cavity; Comparative Example 1 does not contain manganese gluconate, and its antibacterial effect is significantly reduced compared with Example 2, and the good antibacterial effect of manganese gluconate is lost; Comparative Example 2 carboxymethyl deacetylated chitosan and manganese gluconate are not in the form of sol, and the antibacterial effect is weakened; Comparative Examples 3-4 do not contain naringin and fisetin, which changes the composition and ratio of the beneficial ingredients, and significantly affects the antibacterial effect of the toothpaste.

[0081] Test Example 2: Tooth mineralization detection

[0082] Test object: Take 100 mg of each of the toothpastes prepared in Examples 1-3 and Comparative Examples 1-4, and dilute with sterile deionized water to obtain a 5 mg / mL toothpaste solution of the sample to be tested;

[0083] Experimental materials: 35 deciduous teeth of children aged 3 to 6 years old were obtained from a dental hospital. All teeth had no obvious damage on the surface; all teeth had no caries. The crown parts of 36 deciduous teeth of children were cut off and prepared into 35 regular crown tissue blocks with a size of 5×5mm. The crown tissue blocks were ultrasonically cleaned for 25 minutes, and the crown tissue blocks without cracks and defects were selected as the tooth tissue calcification model.

[0084] Experimental method: The prepared crown tissue blocks were demineralized and then randomly divided into 7 groups. They were placed in the toothpaste solution to be tested for 10 minutes and in a neutral buffer solution (20mmol / L HEPES buffer, 2.25mmol / L CaCl2, 1.5mmol / LKH2PO4 pH 7.0) for 5 minutes. The cycle was repeated 3 times a day for 14 days, and then the crown tissue was placed in a neutral buffer solution for 24 hours. Each sample was cut into 350wn thick slices with a slicer. After the tissue slices were polished, they were stained with 0.1mmol / L Rhodamine B fluorescent dye for 1 hour, decolorized on a shaker for 50 minutes, washed 3 times, and blown dry. The sections were sealed with neutral glycerol and the mineral content of the crowns before and after remineralization was analyzed by CLSM, that is, the amount of Rhodamine B fluorescent dye penetrating into the porous dentin was observed, and the fluorescence area and average fluorescence amount before and after remineralization were compared. The larger the difference, the higher the degree of remineralization. The test results are shown in Table 2.

[0085] Table 2 Difference of CLSM measurement parameters before and after mineralization: before mineralization - after mineralization (unit: μm 2 )

[0086]

[0087]

[0088] As can be seen from Table 2, the toothpastes prepared in Examples 1-3 of the present invention are beneficial to the remineralization of teeth. After remineralization, the amount of fluorescence excited by Rhodamine B staining is significantly reduced compared with that before remineralization, indicating that the amount of Rhodamine B fluorescent dye penetrating into porous dentin is significantly reduced, further illustrating that Examples 1-3 are beneficial to the remineralization of the tooth surface, improving the firmness of teeth, and are beneficial to preventing microbial erosion of teeth, which is helpful to the prevention of dental caries. The toothpastes prepared in Comparative Examples 1-4 have a significantly poorer effect on the remineralization of teeth than Examples 1-3, indicating that the components in the formula of the present invention cooperate with each other to promote the remineralization of teeth. The poor remineralization effect of Comparative Example 1 compared with Example 2 indicates that manganese gluconate can provide manganese ions, which combine with hydroxyapatite on the tooth surface to form a protective layer similar to natural teeth, which helps to promote the remineralization process of teeth, thereby reducing the occurrence of tooth decay on the tooth surface.

[0089] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A toothpaste containing probiotics for anti-caries and antibacterial, characterized in that: The composition comprises the following components by weight: 1-5 parts of probiotic freeze-dried powder, 3-8 parts of carboxymethyl chitosan-manganese gluconate sol, 1-5 parts of naringin, 0.5-3 parts of fisetin, 20-45 parts of sorbitol, 10-25 parts of cocamidopropyl betaine, 1-5 parts of cellulose gum and 50-70 parts of deionized water; The probiotic freeze-dried powder is Bifidobacterium lactis freeze-dried powder; the number of live bacteria of Bifidobacterium lactis freeze-dried powder is 100 million bacteria / g; The mass ratio of carboxymethyl chitosan to manganese gluconate in the carboxymethyl chitosan-manganese gluconate sol is 3-5:1; The preparation method of the carboxymethyl chitosan-manganese gluconate sol comprises the following steps: preparing an acetic acid-sodium acetate buffer solution with a pH value of 4-6, dispersing the carboxymethyl chitosan in the acetic acid-sodium acetate buffer solution to obtain a suspension, adding manganese gluconate into the suspension, stirring, filtering, washing, and drying to obtain the carboxymethyl chitosan-manganese gluconate sol.

2. The toothpaste containing probiotics for anti-caries and antibacterial according to claim 1, characterized in that: The invention comprises the following components by weight: 3 parts of probiotic freeze-dried powder, 5 parts of carboxymethyl chitosan-manganese gluconate sol, 3 parts of naringin, 1.5 parts of fisetin, 30 parts of sorbitol, 20 parts of cocamidopropyl betaine, 3 parts of cellulose gum and 60 parts of deionized water.

3. A method for preparing a toothpaste containing probiotics for preventing moths and inhibiting bacteria according to claim 1 or claim 2, characterized in that: The following steps are involved: S1, preparation of carboxymethyl chitosan-manganese gluconate sol; S2, weighing naringin, fisetin, and carboxymethyl chitosan-manganese gluconate sol, and mixing them to obtain a mixture A; S3, weighing sorbitol, cocamidopropyl betaine, cellulose gum, and deionized water, placing them in a stirrer, heating and stirring, to obtain a mixture B; S4, cooling the mixture B to room temperature, adding the mixture A and the probiotic freeze-dried powder to the mixture B, and stirring for 15 to 20 minutes; S5. Perform vacuum degassing to obtain toothpaste containing probiotics for preventing caries and inhibiting bacteria.

4. The method for preparing a toothpaste containing probiotics for anti-caries and antibacterial according to claim 3, characterized in that: The heating temperature in S3 is 60-70° C.; the stirring speed is 1000-2000 r / min, and the stirring time is 25-40 min.

5. The method for preparing a toothpaste containing probiotics for preventing moths and inhibiting bacteria according to claim 3, characterized in that: The vacuum degree of the S5 vacuum degassing is 0.2-0.4 MPa; the degassing time is 50-60 min.

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