A compound antioxidant product containing panax quinquefolium and its preparation method and application

Abstract

The application discloses a kind of American ginseng compound antioxidant products, is prepared from the following mass fraction components extraction: American ginseng 20-30 parts, 15-25 parts of radix rehmanniae preparata, 20-28 parts of dogwood, 15-25 parts of radix scrophulariae, 15-25 parts of gardenia, 8-12 parts of safflower, 18-22 parts of chuanxiong, 20-26 parts of citrus aurantium, 18-25 parts of alocasia, 10-20 parts of polygala, 16-26 parts of Platycladus branchlet, 15-25 parts of hovenia dulcis thunb, after screening, cleaning, crushing, weighing, alcohol extraction, water extraction, concentration and the like step is made.Compared with prior art, the application obtains the beneficial effect: synergistic effect between components, by tonifying qi and invigorating the spleen, nourishing liver and kidney, clearing heart and detoxifying, promoting blood circulation and qi movement, it has the effect of root and original for senile dementia, using reasonable extraction method is beneficial to the extraction of each effective component;Since the components of the application are non-toxic commonly used medicinal materials, there is no harm to the body after use, and there is no side effect.

Description

[0001] This application is a divisional application of application number 202310689265.6, application date June 12, 2023, entitled "A compound antioxidant product containing American ginseng and its preparation method and application". Technical Field

[0002] This invention relates to the field of medical technology, and more specifically to a compound antioxidant product containing American ginseng, its preparation method, and its application. Background Technology

[0003] Alzheimer's disease (AD) is a degenerative disease of the central nervous system that occurs in old age and pre-old age. It is characterized by progressive cognitive impairment and behavioral damage, and its pathological features include Aβ deposition forming neuronal plaques and neurofibrillary tangles formed by hyperphosphorylated Tau protein. The pathogenesis of AD is closely related to Aβ-mediated oxidative stress. Currently, treatment involves drug therapy and non-drug therapies.

[0004] 1. Drug Treatment: Alzheimer's disease, also known as senile dementia, is currently incurable; treatment can only slow its progression. Patients can improve cognitive function by taking medications such as donepezil, rivastigmine, or galantamine, or improve brain metabolism by taking medications such as piracetam, piracetam, or citicoline sodium. Medication treatment should be administered under the guidance of a doctor.

[0005] 2. Non-pharmacological treatment: There are many non-pharmacological treatment methods, including nursing care, nutritional supplementation, cognitive rehabilitation training, or physical exercise, which can improve the patient's memory and cognitive abilities, thereby achieving a therapeutic effect. At the same time, supporting elderly patients to participate in social activities can improve their mental state and thus enhance their quality of life.

[0006] Alzheimer's disease is a common illness among the elderly, severely impacting patients' physical health and quality of life. Alzheimer's patients should not self-medicate; it is recommended that they seek timely medical attention and undergo scientific and rational treatment under the guidance of a doctor, strictly following medical advice to avoid delaying their condition. Current treatment methods have the limitation of treating only the symptoms, not the root cause. Research shows that antioxidants can prevent the occurrence of Alzheimer's disease through multiple pathways. First, antioxidants can neutralize harmful free radicals, reducing oxidative damage to cells and thus protecting cell function. Second, antioxidants can also inhibit inflammation and abnormal neurogenetic activity, slowing down processes such as apoptosis and neuronal degeneration. Furthermore, antioxidants can improve brain cell metabolism, increase the energy supply to brain cells, and help neurons maintain their function. In conclusion, how to provide a treatment method for Alzheimer's disease that is effective and has no toxic side effects is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0007] In view of this, the present invention provides a compound antioxidant product of American ginseng, a composition for treating Alzheimer's disease and a method for preparing the same.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A compound antioxidant product containing American ginseng is prepared from the following components in parts by weight: 20-30 parts American ginseng, 15-25 parts Rehmannia glutinosa, 20-28 parts Cornus officinalis, 15-25 parts Scutellaria baicalensis, 15-25 parts Gardenia jasminoides, 8-12 parts Carthamus tinctorius, 18-22 parts Ligusticum chuanxiong, 20-26 parts Citrus aurantium, 18-25 parts Acorus tatarinowii, 10-20 parts Polygala tenuifolia, 16-26 parts Platycladus orientalis seed, and 15-25 parts Ziziphus jujuba seed.

[0010] The compound antioxidant product containing American ginseng is prepared by extracting the following components in parts by weight: 20 parts American ginseng, 25 parts Rehmannia glutinosa, 20 parts Cornus officinalis, 25 parts Scutellaria baicalensis, 15 parts Gardenia jasminoides, 12 parts Carthamus tinctorius, 18 parts Ligusticum chuanxiong, 26 parts Citrus aurantium, 18 parts Acorus tatarinowii, 20 parts Polygala tenuifolia, 16 parts Platycladus orientalis seed, and 25 parts Ziziphus jujuba seed.

[0011] The preparation method of the American ginseng-containing compound antioxidant product includes the following steps:

[0012] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0013] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, dried, pulverized and sieved, weighed and obtained as powdered drugs.

[0014] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder to obtain mixture A. Add 10 to 12 times the mass of ethanol to mixture A and reflux extract twice, each time for 2 to 3 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain ethanol extract concentrate.

[0015] (4) Mix the remaining medicinal powders to obtain mixture B, add water with a mass of 10 to 20 times that of mixture B, then add the dregs from step (3), soak for 1 to 2 hours, and decoct 3 times, 1 to 2 hours each time;

[0016] (5) Combine the three water decoctions with the alcohol extract concentrate obtained in step (3), concentrate under reduced pressure and then vacuum dry to make extract powder.

[0017] The American ginseng compound antioxidant product is prepared by extracting the following components in parts by weight: 25 parts American ginseng, 20 parts Rehmannia glutinosa, 24 parts Cornus officinalis, 20 parts Scutellaria baicalensis, 20 parts Gardenia jasminoides, 10 parts Carthamus tinctorius, 20 parts Ligusticum chuanxiong, 23 parts Citrus aurantium, 21 parts Acorus tatarinowii, 15 parts Polygala tenuifolia, 20 parts Platycladus orientalis seed, and 20 parts Ziziphus jujuba seed.

[0018] The preparation method of the American ginseng-containing compound antioxidant product includes the following steps:

[0019] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0020] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, dried, pulverized and sieved, weighed and obtained as powdered drugs.

[0021] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder to obtain mixture A. Add 10 to 12 times the mass of ethanol to mixture A and reflux extract twice, each time for 2 to 3 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain ethanol extract concentrate.

[0022] (4) Mix the remaining medicinal powders to obtain mixture B, add water with a mass of 10 to 20 times that of mixture B, then add the dregs from step (3), soak for 1 to 2 hours, and decoct 3 times, 1 to 2 hours each time;

[0023] (5) Combine the three water decoctions with the alcohol extract concentrate obtained in step (3), concentrate under reduced pressure and then vacuum dry to make extract powder.

[0024] The preparation method of the compound antioxidant product containing American ginseng has the following steps: the drying temperature in step (2) is 40-60℃, the sieving is 80-100 mesh, and the volume concentration of ethanol in step (3) is 80-90%.

[0025] The preparation method of the compound antioxidant product containing American ginseng also includes adding the extract powder to excipients to prepare tablets, capsules or granules.

[0026] The excipients in the preparation method of the compound antioxidant product containing American ginseng are one or more of dextrin, mannitol, sucrose, xylitol, and starch.

[0027] The application of the compound antioxidant product containing American ginseng in the preparation of antioxidant drugs.

[0028] The application of the compound antioxidant product containing American ginseng in the preparation of medicines to improve memory.

[0029] The application of the compound antioxidant product containing American ginseng in the preparation of drugs for treating Alzheimer's disease.

[0030] American ginseng, Poria cocos, Dioscorea opposita, Rehmannia glutinosa, Cornus officinalis, Scutellaria baicalensis, Gardenia jasminoides, Carthamus tinctorius, Ligusticum chuanxiong, Citrus aurantium, Acorus tatarinowii, Polygala tenuifolia, Platycladus orientalis seed, and Ziziphus jujuba seed all conform to the Chinese Pharmaco

[0031] Poria cocos is sweet, bland, and neutral in nature. It enters the heart, spleen, and kidney meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen, and calming the mind. It is used for edema, diarrhea, difficulty urinating, phlegm retention, palpitations, and insomnia.

[0032] Rehmannia glutinosa (processed) is sweet and slightly warm in nature, and enters the liver and kidney meridians. It nourishes blood and yin, replenishes essence and marrow, and is suitable for blood deficiency, irregular menstruation in women, liver and kidney yin deficiency, diabetes, and deficiency of essence and blood. Rehmannia glutinosa is an important medicine for nourishing the liver and kidneys, not only nourishing blood and yin but also replenishing essence and marrow. Traditional Chinese medicine believes that essence and blood are the most fundamental material basis in the human body. Sufficient essence and blood result in normal liver and kidney function, and a strong and healthy body. Conversely, insufficient essence and blood can lead to dizziness, blurred vision, and weakness in the lower back and knees. Modern medical research has confirmed that Rehmannia glutinosa contains sitosterol, mannitol, rehmannia glutinosa, sugars, and various vitamins and minerals. Rehmannia glutinosa has the effects of inhibiting lipid peroxidation, increasing antioxidant enzyme activity, lowering blood pressure and lipids, inhibiting thrombosis, and improving myocardial ischemia.

[0033] Chuanxiong (Ligusticum striatum) is pungent and warm in nature. It enters the liver, gallbladder, and pericardium meridians. It has the effects of promoting blood circulation, regulating qi, dispelling wind, and relieving pain. It is used to soothe nerves, relieve headaches caused by wind, abdominal pain due to masses, stabbing pain in the chest and ribs, and headaches.

[0034] Salvia miltiorrhiza has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, clearing the heart and relieving irritability, and cooling the blood. It is used to treat abdominal and hypochondriac pain, insomnia due to heart palpitations, irregular menstruation, and swelling and pain.

[0035] Goji berries are neutral in nature and sweet in taste. They enter the liver, kidney, and lung meridians. They nourish the liver and kidneys, improve eyesight, and moisten the lungs. They are used for dizziness, blurred vision, seminal emission, thirst, and cough due to yin deficiency.

[0036] American ginseng is the dried root of *Panax quinquefolium* L., a plant belonging to the Araliaceae family. It is a cultivated product, harvested in autumn, washed, and sun-dried or dried at low temperature. It enters the heart, lung, and kidney meridians. It is sweet and slightly bitter, and cool in nature. It tonifies qi and nourishes yin, clears heat and generates fluids. It is used for qi and yin deficiency, internal heat, cough with phlegm and blood, fatigue due to deficiency heat, thirst, and dry mouth and throat.

[0037] The principles of Traditional Chinese Medicine (TCM) in treating Alzheimer's disease are based on the understanding of the etiology and pathogenesis of dementia. TCM believes that the brain is the residence of the primordial spirit, and memory resides in the brain, not the heart. In old age, memory loss indicates a gradual depletion of brain marrow. A person's essence and will are stored in the kidneys; insufficient essence leads to a weakened will and qi, which cannot ascend to the heart, thus causing confusion and forgetfulness. TCM also believes that dementia can gradually develop from conditions without phlegm, due to stagnation, frustration, excessive thinking, or fright. Deficiency of heart qi leads to a clouded spirit and the production of phlegm; phlegm obstructing the heart orifices causes forgetfulness. Blood stasis in the heart also contributes to forgetfulness. From the above discussion, it can be seen that TCM believes the location of Alzheimer's disease is in the brain, closely related to imbalances in the heart, liver, spleen, and kidneys. It is caused by pathogenic factors such as qi, blood, phlegm, stagnation, and heat, resulting in depletion of essence and blood in the elderly, depletion of brain marrow, insufficient primordial qi, and an imbalance of yin and yang. The characteristics include deficiency of the heart, kidneys, and spleen, and internal obstruction of phlegm and blood stasis. The heart, as the monarch of the organs, governs the mind; insufficient qi and blood lead to malnourishment of the brain, resulting in mental confusion, delirium, and forgetfulness. The kidneys are the foundation of innate essence, governing bones, producing marrow, which in turn connects to the brain; the essence of the kidneys is a crucial material basis for the brain. Insufficient kidney essence leads to malnourishment of the brain, resulting in yin-yang imbalance, confusion, forgetfulness, slow movement, and sluggish reactions. Secondly, the seven emotions injure the liver, causing stagnation of liver qi, or spleen dysfunction and water retention, easily generating phlegm and fire. The accumulation of phlegm and blood stasis forms pathogenic factors, obstructing the five internal organs and causing dementia.

[0038] This invention posits that Alzheimer's patients are often elderly and chronically ill, suffering from qi and blood deficiency, leading to spleen and kidney deficiency, insufficient production of vital energy, and empty marrow. Therefore, American ginseng, Poria cocos, and Dioscorea opposita are used to invigorate qi and strengthen the spleen, while Rehmannia glutinosa and Cornus officinalis nourish the liver and kidneys, serving as the principal herbs. Furthermore, since Alzheimer's disease is often caused by prolonged qi stagnation, which transforms into fire and scorches yin, or by blood stasis obstructing the brain's collaterals, preventing the brain's qi from connecting with the internal organs, resulting in forgetfulness, Scutellaria baicalensis and Gardenia jasminoides are used to clear the heart and detoxify, while Carthamus tinctorius, Ligusticum chuanxiong, and Citrus aurantium are used to invigorate blood and promote qi circulation, serving as the assistant herbs. Moreover, since a weak spleen and stomach can lead to phlegm accumulation in the chest, obscuring the clear mind, Acorus tatarinowii and Polygala tenuifolia are used as adjuvant herbs to resolve phlegm and open the orifices, while Platycladus orientalis seed and Ziziphus jujuba seed are used as guiding herbs to nourish the heart and calm the mind. Targeting the irritability and restlessness of Alzheimer's patients, the entire formula has been experimentally studied and has antioxidant effects, improves memory, and can treat Alzheimer's disease. Detailed Implementation

[0039] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] The reagents required for this invention are conventional experimental reagents, purchased from commercially available channels; the experimental methods not mentioned are conventional experimental methods, and will not be described in detail here.

[0041] Example 1

[0042] Formula: American ginseng 20g, Rehmannia glutinosa 25g, Cornus officinalis 20g, Scutellaria baicalensis 25g, Gardenia jasminoides 15g, Carthamus tinctorius 12g, Ligusticum chuanxiong 18g, Citrus aurantium 26g, Acorus tatarinowii 18g, Polygala tenuifolia 20g, Platycladus orientalis seed 16g, Ziziphus jujuba seed 25g.

[0043] Preparation steps:

[0044] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0045] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, then dried (drying temperature 40℃), pulverized, passed through an 80-mesh sieve, weighed, and powdered drugs are obtained.

[0046] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder, add 10 times the weight of the medicinal materials in 80% ethanol, and reflux extract twice, each time for 2 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the concentrated alcohol extract.

[0047] (4) Mix the remaining medicinal powders, add water at 20 times the weight of the medicinal materials, add the dregs obtained in step (3), soak for 1 hour, decoct 3 times, 2 hours each time;

[0048] (5) Combine the three decoctions with the concentrated alcohol extract obtained in step (3), concentrate under reduced pressure and then vacuum dry to make a dry extract powder. Add dextrin to prepare granules.

[0049] Example 2

[0050] Formula: American ginseng 30g, Rehmannia glutinosa 15g, Cornus officinalis 28g, Scutellaria baicalensis 15g, Gardenia jasminoides 25g, Carthamus tinctorius 8g, Ligusticum chuanxiong 22g, Citrus aurantium 20g, Acorus tatarinowii 25g, Polygala tenuifolia 10g, Platycladus orientalis seed 26g, Ziziphus jujuba seed 15g.

[0051] Preparation steps:

[0052] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0053] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, then dried (drying temperature 60℃), pulverized, passed through a 100-mesh sieve, weighed, and powdered drugs are obtained.

[0054] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder, add 90% ethanol at 12 times the weight of the medicinal materials, and reflux extract twice, each time for 3 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the concentrated ethanol extract.

[0055] (4) Mix the remaining medicinal powders, add water at 10 times the weight of the medicinal materials, then add the dregs obtained in step (3), soak for 1 hour, and decoct 3 times, 1 hour each time;

[0056] (5) Combine the three decoctions with the concentrated alcohol extract obtained in step (3), concentrate under reduced pressure and then vacuum dry to make a dry extract powder. Add dextrin to prepare granules.

[0057] Example 3

[0058] Formula: American ginseng 20g, Rehmannia glutinosa 25g, Cornus officinalis 20g, Scutellaria baicalensis 25g, Gardenia jasminoides 15g, Carthamus tinctorius 12g, Ligusticum chuanxiong 18g, Citrus aurantium 26g, Acorus tatarinowii 18g, Polygala tenuifolia 20g, Platycladus orientalis seed 16g, Ziziphus jujuba seed 25g.

[0059] Preparation steps:

[0060] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0061] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, then dried (drying temperature 50℃), pulverized, passed through a 90-mesh sieve, weighed, and powdered drugs are obtained.

[0062] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder, add 12 times the weight of the medicinal materials of 85% ethanol, and reflux extract twice, each time for 2.5 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the concentrated ethanol extract.

[0063] (4) Mix the remaining medicinal powders, add water at 20 times the weight of the medicinal materials, then add the dregs obtained in step (3), soak for 2 hours, and decoct 3 times, 1 hour each time;

[0064] (5) Combine the three decoctions with the concentrated alcohol extract obtained in step (3), concentrate under reduced pressure and then vacuum dry to make a dry extract powder. Add mannitol to prepare tablets.

[0065] Example 4

[0066] Formula: American ginseng 20g, Rehmannia glutinosa 25g, Cornus officinalis 20g, Scutellaria baicalensis 25g, Gardenia jasminoides 15g, Carthamus tinctorius 12g, Ligusticum chuanxiong 18g, Citrus aurantium 26g, Acorus tatarinowii 18g, Polygala tenuifolia 20g, Platycladus orientalis seed 16g, Ziziphus jujuba seed 25g.

[0067] Preparation steps:

[0068] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0069] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, then dried (drying temperature 60℃), pulverized, passed through a 100-mesh sieve, weighed, and powdered drugs are obtained.

[0070] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder, add 90% ethanol at 10 times the weight of the medicinal materials, and reflux extract twice, each time for 2 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the concentrated ethanol extract.

[0071] (4) Mix the remaining medicinal powders, add water at 20 times the weight of the medicinal materials, add the dregs obtained in step (3), soak for 1 hour, decoct 3 times, 2 hours each time;

[0072] (5) Combine the three decoctions with the concentrated alcohol extract obtained in step (3), concentrate under reduced pressure and then vacuum dry to make a dry extract powder. Add xylitol to prepare capsules.

[0073] Example 5

[0074] Formula: American ginseng 20g, Rehmannia glutinosa 25g, Cornus officinalis 20g, Scutellaria baicalensis 25g, Gardenia jasminoides 15g, Carthamus tinctorius 12g, Ligusticum chuanxiong 18g, Citrus aurantium 26g, Acorus tatarinowii 18g, Polygala tenuifolia 20g, Platycladus orientalis seed 16g, Ziziphus jujuba seed 25g.

[0075] Preparation steps:

[0076] (1) Selection and weighing: Select raw materials carefully to remove impurities and substandard products;

[0077] (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, then dried (drying temperature 60℃), pulverized, passed through an 80-mesh sieve, weighed, and powdered drugs are obtained.

[0078] (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder, add 12 times the weight of the medicinal materials in 80% ethanol, and reflux extract twice, each time for 2 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the concentrated alcohol extract.

[0079] (4) Mix the remaining medicinal powders, add water at 20 times the weight of the medicinal materials, add the dregs obtained in step (3), soak for 1 hour, decoct 3 times, 2 hours each time;

[0080] (5) Combine the three water decoctions with the alcohol extract concentrate obtained in step (3), concentrate under reduced pressure and then vacuum dry to make extract powder, add starch and prepare granules.

[0081] Experimental Example 1: A pharmacodynamic study of the effect of this invention on a scopolamine-induced mouse model of memory acquisition impairment. The methods and results are as follows:

[0082] 1. Experimental Materials

[0083] Seventy-two male Kunming mice, weighing 18-22g, were kept at a constant temperature (21-23℃), constant humidity (45-65%), artificial day and night, fed with ordinary feed, and given free access to water. They were randomly divided into six groups: a control group, a model group, a positive control group (donepezil hydrochloride 0.65mg / kg), and the granules prepared in Example 1 of this invention (low-dose group 1g / kg bw, medium-dose group 2g / kg bw, high-dose group 4g / kg bw). The control and model groups were given the same amount of physiological saline. A memory test was conducted 10 minutes later, using the step-down test and water maze test to assess the mice's learning and memory abilities.

[0084] 2. Experimental methods and observation indicators.

[0085] 2.1 Jumping Platform Method: Mice in each group were placed on a jumping platform device. They were first allowed to acclimatize to the voltage environment for 3 minutes, followed by continuous electrical stimulation at 36V for 5 minutes. The normal escape response of mice after receiving the electric shock was to jump onto the platform. The number of times the animals jumped off the platform within 5 minutes was recorded as the number of errors during the training period. This was performed in parallel during training. Recording was repeated 24 hours later, noting the time of the mouse's first jump (latency period) and the number of jumps within 5 minutes (i.e., the number of errors), which served as a memory indicator.

[0086] 2.2 Water Maze Method: The water temperature in the Y-shaped water maze box for mice was maintained at 25-27℃, and the water depth was approximately 1 cm. Mice in different groups were placed into the water maze for training. During training, the mice were gently placed into the water from the end of the long arm, tail to tail, with reaching the platform within 15 seconds as the correct response. After reaching the platform, the mice rested for 15 seconds before the training was repeated 6 times. The time from entry into the water to reaching the platform and the number of correct responses were recorded. Memory retention was tested after 24 hours, and memory consolidation was tested after 48 hours.

[0087] 2.3 Detection of SOD activity and MDA level in mouse brain tissue: After the water maze experiment, 10 mice were randomly selected from each group. After anesthesia with 10% chloral hydrate via intraperitoneal injection, brain tissue was collected, lysed, homogenized, centrifuged at 12,000 rpm for 10 minutes, and the supernatant was collected. The MDA level and SOD activity in mouse brain tissue were detected by ELISA.

[0088] 3 Results

[0089] 3.1 The test results of the platform jumping method are shown in Table 1.

[0090] Table 1. Results of the step-jump test in mice of each group (x±s, n=10)

[0091] Group Latency period (s) of the diving platform Number of errors (n) normal control group 234.5±79.6 1.2±1.34 Model control group 57.8±53.8 14.8±7.19 Positive control group 198.4±84.2* 3.7±2.63* The low-dose group of the present invention 156.9±57.4* 7.4±4.32* In this invention, the dosage group 187.5±47.5* 5.6±2.98* High-dose group of the present invention 204.7±83.8* 3.50±2.42*

[0092] Note: *P<0.05 compared with the model control group.

[0093] The results of the platform jumping test showed that the latency period of the mice in the model control group was significantly shortened and the number of errors was significantly increased; while the drugs administered in this invention significantly prolonged the latency period of the mice in the platform jumping test and significantly reduced the number of errors, and this was dose-dependent.

[0094] 3.2 The results of the water maze test are shown in Table 2.

[0095] Table 2. Results of the water maze test in each group of mice (x±s, n=10)

[0096]

[0097]

[0098] Note: Compared with the model control group, *P<0.05, **P<0.01.

[0099] The results of the water maze test over three consecutive days showed that, compared with the normal control group, the time required for mice in the model group to navigate the maze was significantly longer (P<0.01). The time required for mice in each dosage group of the present invention to navigate the maze was significantly shorter than that in the model group (P<0.05 or P<0.01), indicating a dose-related relationship.

[0100] 3.3 The results of the determination of SOD activity and MDA level in mouse brain tissue are shown in Table 3.

[0101] Table 3. Determination of SOD activity and MDA levels in mouse brain tissue (x±s, n=10)

[0102] Group SOD (ng / mL) MDA (nmol / mL) normal control group 3.56±0.78 0.96±0.09 Model control group 2.23±0.26 1.43±0.34 Positive control group 3.42±1.53* 1.14±0.53* The low-dose group of the present invention 2.56±0.34* 1.34±0.94* In this invention, the dosage group 3.09±1.02* 1.25±0.45* High-dose group of the present invention 3.27±1.24** 1.17±0.29**

[0103] Note: Compared with the model control group, *P<0.05, **P<0.01.

[0104] Experimental results on SOD activity and MDA levels in mouse brain tissue showed that, compared with the blank group, the model control group had significantly increased MDA levels and significantly decreased SOD activity; compared with the model group, all groups of this invention showed increased SOD activity (P<0.05, P<0.01). This indicates that the therapeutic effect of this invention on Alzheimer's disease may be due to its antioxidant properties.

[0105] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A compound antioxidant product containing American ginseng, characterized in that, It is prepared from the following components in parts by weight: American ginseng 20 parts, Rehmannia glutinosa 25 parts, Cornus officinalis 20 parts, Scutellaria baicalensis 25 parts, Gardenia jasminoides 15 parts, Carthamus tinctorius 12 parts, Ligusticum chuanxiong 18 parts, Citrus aurantium 26 parts, Acorus tatarinowii 18 parts, Polygala tenuifolia 20 parts, Platycladus orientalis seed 16 parts, and Ziziphus jujuba seed 25 parts. The preparation method includes the following steps: (1) Selection and weighing: Select raw materials to remove impurities and substandard products; (2) Cleaning, drying and pulverizing: The raw materials obtained in step (1) are cleaned, dried, pulverized and sieved, weighed and obtained as powdered drugs. (3) Mix safflower powder, chuanxiong powder, cypress seed powder and jujube seed powder to obtain mixture A. Add 10 to 12 times the mass of ethanol to mixture A and reflux extract twice, each time for 2 to 3 hours. Combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain ethanol extract concentrate. (4) Mix the remaining medicinal powders to obtain mixture B, add water with a mass of 10 to 20 times that of mixture B, then add the dregs from step (3), soak for 1 to 2 hours, and decoct 3 times, 1 to 2 hours each time; (5) Combine the three water decoctions with the alcohol extract concentrate obtained in step (3), concentrate under reduced pressure and then vacuum dry to make extract powder. In step (2) of the preparation method, the drying temperature is 40-60℃ and the sieving is through an 80-100 mesh sieve. In step (3) of the preparation method, the volume concentration of ethanol is 80-90%. The preparation method also includes adding the extract powder to an excipient to prepare tablets, capsules or granules. The excipient in the preparation method is one or more of dextrin, mannitol, sucrose, xylitol and starch.

2. The application of the ginseng-containing compound antioxidant product as described in claim 1 in the preparation of antioxidant drugs.

3. The application of the ginseng-containing compound antioxidant product as described in claim 1 in the preparation of products that regulate memory function.

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