A bodily fluid-based biological detection device and method

Abstract

The application discloses a kind of biological detection device and method based on body fluid, device includes successively from lower to upper water filter layer, molecular imprinting polymer layer and flexible circuit board layer, and biochemical sensor is integrated in flexible circuit board layer;In a state, water filter layer filters water in body fluid, molecular imprinting polymer layer identifies and combines biomarker in the body fluid after filtration, the combination of biomarker and molecular imprinting polymer layer causes the current of biochemical sensor to change, to change physical signal into biological signal in body fluid;The application is a kind of biological detection device and method based on body fluid with high sensitivity, stable detection result.

Description

Technical Field

[0001] This invention relates to the field of biomarker detection technology, specifically a biological detection device and method based on body fluids. Background Technology

[0002] Biological fluids refer to various fluids secreted by the human body, such as saliva, blood, urine, sputum, sweat, gastric juice, as well as semen and vaginal discharge. With the advancement of medicine, testing these bodily fluids can not only help examinees identify diseased areas but also detect potential health problems in advance.

[0003] Currently, blood is the most common biological fluid sample. However, existing blood sample testing technologies have the following drawbacks: 1. Blood sample collection is invasive; 2. Blood samples need to be tested and analyzed in a laboratory, which inevitably places high demands on the testing environment and instruments; 3. Testing is not continuous.

[0004] In recent years, an increasing number of researchers have focused on studying non-invasive, convenient, real-time, and continuous biological fluids as samples for health monitoring. Chinese Patent Publication No. CN111671437A discloses a wearable sweat detection system, method, and wearable device. The system includes a microfluidic chip, an electrochemical electrode, a circuit acquisition system, and a mobile terminal system. A sweat collection section is located on the side of the microfluidic chip that contacts the skin, and a sweat concentration chamber is located on the other side of the microfluidic chip, which is in contact with the electrochemical electrode. The circuit acquisition system converts the electrical signals of the sweat components detected by the electrochemical electrode into digital signals and sends them to the mobile terminal system. The mobile terminal system receives, analyzes, and processes the digital signals before outputting the sweat detection results. This invention provides a wearable sweat detection system, method, and wearable device that is simple to operate, convenient to carry, and can collect, detect, analyze, and output the sweat of the subject in real time, thereby enabling real-time monitoring and timely warning of human health status information. However, the detection system provided by this technical solution has the following drawbacks: 1. It does not examine biomarkers; 2. It uses microfluidics to collect sweat samples, which requires a certain amount of sweat.

[0005] In addition, sweat testing can avoid the drawbacks of blood testing, but existing sweat testing devices still have shortcomings such as low sensitivity and unstable test results. Summary of the Invention

[0006] To address the above problems, the present invention provides a biological detection device and method based on body fluids that has high sensitivity and stable detection results.

[0007] To achieve this objective, the present invention provides the following technical solution:

[0008] In a first aspect, the present invention provides a biological detection device based on body fluid, comprising, from bottom to top, a water filtration layer, a molecularly imprinted polymer layer, and a flexible circuit board layer, wherein the flexible circuit board layer integrates a biochemical sensor; in one state, the water filtration layer filters water from the body fluid, the molecularly imprinted polymer layer identifies and binds to biomarkers in the filtered body fluid, and the binding of the biomarkers to the molecularly imprinted polymer layer causes a change in the current of the biochemical sensor, thereby converting the biological signal in the body fluid into a physical signal.

[0009] In this invention, the filter layer can filter water from bodily fluids, such as sweat, which contains approximately 99% water and very little biomarker. Through extensive experimentation, the inventors of this application have discovered that filtering water from the sample before testing can increase the sensitivity and accuracy of the test results.

[0010] In this invention, the molecularly imprinted polymer layer can precisely bind to the biomarker to be detected, thereby making the detection results of the detection device more accurate.

[0011] In this invention, the flexible circuit board layer can integrate a variety of electronic components, and its functions can be expanded according to the actual needs of the user.

[0012] In this invention, the detection device includes, but is not limited to, from bottom to top, a water filter layer, a molecularly imprinted polymer layer, and a flexible circuit board layer. In practical use, a housing can be added outside the circuit board layer, or a water filter layer can be loaded outside the circuit board layer to protect the electronic components on the circuit board layer.

[0013] Preferably, the flexible circuit board layer also integrates a humidity sensor. In one state, the humidity sensor monitors the humidity around the detection device and transmits the humidity information to the biochemical sensor in real time. The biochemical sensor uses the humidity data as a volume parameter of body fluid and, in combination with the biomarker data transmitted by the molecularly imprinted polymer layer, analyzes and calculates the concentration of the biomarker.

[0014] Preferably, the humidity sensor includes an MXene-based humidity sensor.

[0015] In this invention, the humidity sensor can be any one or more small sensors, including but not limited to MXene-based humidity sensors. The choice of a small sensor is based on controlling the overall size of the detection device to facilitate its use, particularly in wearable or adhesive applications.

[0016] Preferably, the filter layer comprises a UHMWPE membrane.

[0017] In this paper, the filter layer can be any one or more compounds with high porosity and high air permeability. Based on the application of the detection device in wearable or adhesive applications, the filter layer is made of green materials that are non-toxic and harmless to organisms.

[0018] Preferably, the biochemical sensor includes a biochemical sensor based on an organic electrochemical transistor.

[0019] Preferably, it also includes a smart terminal, which is connected to the flexible circuit board layer via a network signal; more preferably, the smart terminal includes a mobile phone, computer, iPad, etc.

[0020] Preferably, the detection device is wearable or adhesive; more preferably, when the detection device is wearable, the detection device further includes a collar; when the detection device is adhesive, the detection device does not require the use of additional adhesive and can be adhered to the skin solely by van der Waals forces.

[0021] In this invention, the detection device is wearable or adhesive, which has the advantages of being easy to use and having good market prospects.

[0022] Preferably, the biomarkers include biometabolites, hormones, electrolytes, and proteins; more preferably, the biometabolites include, but are not limited to, lactic acid, uric acid, and glucose; the hormones include, but are not limited to, cortisol; the electrolytes include, but are not limited to, sodium, chloride, and hydrogen ions; and the proteins include, but are not limited to, C-reactive protein.

[0023] Preferably, the bodily fluids include sweat and sweat vapor.

[0024] In this invention, by detecting biomarkers in sweat vapor, interference with the test results from unconscious sweating can be avoided.

[0025] Preferably, the thickness of the detection device is 100-500nm; more preferably, the thickness of the detection device is 150nm, 200nm, 250nm, 300nm, 350nm, 400nm, or 450nm.

[0026] Preferably, the detection device further includes an early warning system, which is programmed with a threshold. When the result detected by the biochemical sensor is higher or lower than the threshold, the early warning system activates an alarm. More preferably, the early warning system is connected to the smart terminal of a hospital or family doctor via a wireless network. After the early warning system activates an alarm, the smart terminal of the hospital or family doctor obtains the early warning information in real time and intervenes promptly.

[0027] A second aspect of the present invention provides a biological detection method based on body fluids, comprising the following steps:

[0028] S1. Body fluids are filtered through a water layer to remove water.

[0029] S2, the molecularly imprinted polymer layer recognizes and binds to biomarkers in filtered body fluids;

[0030] S3. The binding of biomarkers to the molecularly imprinted polymer layer causes a change in the current of the biochemical sensor, thereby converting biological signals in body fluids into physical signals.

[0031] S4. Biochemical sensors analyze the physical signals of biomarkers in body fluids.

[0032] Preferably, the bodily fluids include sweat and sweat vapor, and the method further includes the following step: correcting the results of the test of the subject's sweat biomarkers using the results of the detection of biomarkers of sweat vapor under conditions of involuntary sweating.

[0033] Preferably, the detection method employs a body fluid-based biological detection device provided by the present invention. The detection device comprises, from bottom to top, a water filtration layer, a molecularly imprinted polymer layer, and a flexible circuit board layer. The flexible circuit board layer integrates a biochemical sensor. In one state, the water filtration layer filters water from the body fluid, and the molecularly imprinted polymer layer identifies and binds to biomarkers in the filtered body fluid. The binding of the biomarkers to the molecularly imprinted polymer layer causes a change in the current of the biochemical sensor, thereby converting the biological signal in the body fluid into a physical signal.

[0034] Preferably, the flexible circuit board layer also integrates a humidity sensor. In one state, the humidity sensor monitors the humidity around the detection device and transmits the humidity information to the biochemical sensor in real time. The biochemical sensor uses the humidity data as a volume parameter of body fluid and, in combination with the biomarker data transmitted by the molecularly imprinted polymer layer, analyzes and calculates the concentration of the biomarker.

[0035] Preferably, the humidity sensor includes an MXene-based humidity sensor.

[0036] Preferably, the filter layer comprises a UHMWPE membrane.

[0037] Preferably, the biochemical sensor includes a biochemical sensor based on an organic electrochemical transistor.

[0038] Preferably, it also includes a smart terminal, which is connected to the flexible circuit board layer via a network signal.

[0039] Preferably, the detection device is wearable or adhesive.

[0040] Preferably, the biomarkers include biological metabolites, hormones, electrolytes, and proteins.

[0041] Preferably, the thickness of the detection device is 100-500 nm.

[0042] Compared with the prior art, the beneficial effects and significant progress of applying the technical solution of the present invention are as follows:

[0043] 1. The body fluid-based biological detection device of the present invention is thinner, at 100-500 nm, and is more suitable for wearable or adhesive applications;

[0044] 2. The body fluid-based biological detection device of the present invention can remove water from body fluids through a filter layer, while biomarkers in sweat are retained in the molecularly imprinted polymer layer through a thin membrane and bind to the molecularly imprinted polymer layer. Thus, biomarkers are obtained more accurately through the molecularly imprinted polymer layer, ultimately making the detection device more sensitive and accurate. In addition, most existing products on the market use microfluidics to detect sweat, while the device of the present invention can use sweat vapor to detect biomarkers, and the detection results of the present invention are more accurate.

[0045] 3. The biological detection device based on body fluid of the present invention adds a humidity sensor that can be used to measure the concentration of biomarkers. The biological detection device based on body fluid of the present invention can also be used to detect involuntary sweating. By comparing the data of microbial markers in sweat and sweat vapor, the interference of involuntary sweating on the data can be avoided. Attached Figure Description

[0046] To more clearly illustrate the technical solution of the present invention, the accompanying drawings used in the embodiments of the present invention will be briefly introduced below.

[0047] Obviously, the accompanying drawings described below are only some of the drawings of the embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort, but these other drawings are also within the scope of the drawings required for the embodiments of the present invention.

[0048] Figure 1 This is a schematic diagram of the structure of a biological detection device based on body fluids in Embodiment 1 of the present invention;

[0049] Figure 2 This is a partial structural schematic diagram of a biological detection device based on body fluids according to Embodiment 1 of the present invention;

[0050] Figure 3 This is a diagram illustrating the usage state of the prior art body fluid detection device in Embodiment 2 of the present invention;

[0051] Figure 4 This is a microscopic image of the materials used in a body fluid-based biological detection device according to Embodiment 2 of the present invention.

[0052] Figure 5 This is a skin-friendliness experiment diagram of a body fluid-based biological detection device in Embodiment 3 of the present invention;

[0053] Figure 6 This is a schematic diagram of a biological detection device based on body fluids, as shown in Embodiments 4 and 5 of the present invention.

[0054] Figure 7 The flowcharts are for a body fluid-based biological detection device according to embodiments 4 and 5 of the present invention.

[0055] Reference numerals: 1. Filter layer; 2. Molecularly imprinted polymer layer; 3. Flexible circuit board layer; 3.1. Biochemical sensor; 3.2. Humidity sensor. Detailed Implementation

[0056] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, it should be understood that after reading this invention, those skilled in the art can make various alterations and modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

[0057] To better understand this invention, the technical terms used in this invention will be explained below.

[0058] Biochemical sensors, also known as electrochemical sensors, are devices or apparatuses that can respond to biochemical quantities and convert them into measurable physical signals (optical signals, electrical signals, etc.) according to certain rules. Biochemical sensors are a research field formed by the interpenetration of multiple disciplines such as biology, chemistry, physics, electronics, medicine, and semiconductor technology. They are characterized by good selectivity, high sensitivity, fast analysis speed, and low cost, enabling online continuous monitoring in complex systems. They are widely used in fields such as chemistry, life sciences, biomedicine, environmental monitoring, food, pharmaceuticals, and the military. Structurally, a biosensor mainly consists of two parts: a sensitive membrane (a highly sensitive modified membrane) and a transducer. The sensitive membrane reacts with the analyte, identifying the measured biological or chemical quantity, thus converting the change in the measured quantity into a measurable change. The transducer converts the measured signal sensed by the sensitive membrane into a physical signal for easy measurement.

[0059] Molecularly imprinted polymers refer to polymers synthesized using natural or synthetic compounds to simulate biological systems for molecular recognition research. These polymers possess specific recognition and selective adsorption capabilities. Molecular imprinting is achieved through the following methods: (1) binding imprinted molecules to functional monomers via covalent or non-covalent bonds to form a template-monomer complex; (2) adding a crosslinker to the complex, which, under the initiation of an initiator, heat, or light, induces a polymerization reaction around the imprinted molecule-monomer complex. During this process, the polymer chains "capture" the template molecule and monomer complex into the polymer's three-dimensional structure through free radical polymerization; (3) extracting or dissociating the imprinted molecules from the polymer using appropriate methods to form binding sites that recognize the imprinted molecules.

[0060] Humidity sensors based on MXene utilize MXene materials, which are a class of metal carbide and metal nitride materials with a two-dimensional layered structure, resembling stacked potato chips. The chemical formula of MXene is M... n+1 AX nIn this model (n = 1–3), M represents an early transition metal, such as Sc, Ti, Zr, V, Nb, Cr, or Mo; A typically represents Group III and Group IV chemical elements; and X represents C or N. A humidity sensor based on MXene utilizes the excellent hydrophilicity and conductivity of MXene. MXene nanosheets are coated onto chitosan-modified TPU electrospun nanofibers via electrostatic interactions to prepare an MXene / TPU composite film, and a humidity sensor is fabricated based on this film. A joint research team from the First Affiliated Hospital of Xi'an Jiaotong University and the School of Advanced Materials and Nanotechnology of Xi'an University of Electronic Science and Technology published a paper in the journal *Advanced Sensor Research* entitled "MXene / TPU Composite Film for Humidity Sensing and Human Respiration Monitoring." This research utilizes the excellent hydrophilicity and conductivity of MXene, and coats MXene nanosheets onto chitosan-modified TPU electrospun nanofibers via electrostatic interactions to prepare an MXene / TPU composite film, and a humidity sensor is fabricated based on this film. Based on the principle that changes in water molecule concentration affect the spacing of MXene nanosheets and thus change the tunnel resistance, the MXene / TPU humidity sensor exhibits many characteristics such as fast response speed (12s), wide humidity response range (11%-94% relative humidity (RH)), low hysteresis (<7%RH) and high repeatability.

[0061] Biochemical sensors based on organic electrochemical transistors (OECTs) are characterized by high sensitivity. OECT-based sensors are typically controlled by two interfaces: gate / electrolyte and electrolyte / channel; changes at either interface will alter the device's performance.

[0062] UHMWPE membrane, or ultra-high molecular weight polyethylene for short, is characterized by low cost, flexibility, stretchability, and porosity. Chinese patent CN113263747B discloses a novel, robust, and mechanically flexible 100nm UHMWPE film with a polygonal pore structure that can be easily prepared using an initial low-winding UHMWPE film. This novel nanofilm boasts a tensile strength of up to 900 MPa and an elongation of 26%, making it widely applicable in many important technological fields.

[0063] Example 1

[0064] like Figure 1As shown, a biological detection device based on body fluid includes, from bottom to top, a water filtration layer 1, a molecularly imprinted polymer layer 2, and a flexible circuit board layer 3. The flexible circuit board layer 3 integrates a biochemical sensor 3.1. The water filtration layer 1 filters water from the body fluid, and the molecularly imprinted polymer layer 2 identifies and binds to biomarkers in the filtered body fluid. The binding of biomarkers to the molecularly imprinted polymer layer 2 causes a change in the current of the biochemical sensor 3.1, thereby converting the biological signal in the body fluid into a physical signal.

[0065] like Figure 2 As shown, the flexible circuit board layer 3 also integrates a humidity sensor 3.2. The humidity sensor 3.2 monitors the humidity around the detection device and transmits the humidity information to the biochemical sensor 3.1 in real time. The biochemical sensor 3.1 uses the humidity data as a volume parameter of body fluid and, in combination with the biomarker data transmitted by the molecularly imprinted polymer layer 2, analyzes and calculates the concentration of the biomarker.

[0066] In this embodiment, the humidity sensor includes an MXene-based humidity sensor.

[0067] In this embodiment, the filter layer includes a UHMWPE membrane.

[0068] In this embodiment, the biochemical sensor includes a biochemical sensor based on an organic electrochemical transistor.

[0069] In this embodiment, a smart terminal is also included, which is connected to the flexible circuit board layer via a network signal.

[0070] In this embodiment, the detection device is an adhesive type.

[0071] In this embodiment, biomarkers include biological metabolites, hormones, electrolytes, and proteins.

[0072] In this embodiment, body fluids include sweat and sweat vapor.

[0073] In this embodiment, the thickness of the detection device is 150 nm.

[0074] Example 2

[0075] A comparative experiment was conducted on the body fluid-based biological detection device (experimental group) of Example 1 and a commercially available sweat detection device (control group). The devices of the experimental group and the control group were attached to the left and right arms of the subjects, respectively. The time of first detection of biological signal, the total amount of biological signal detected, and the condition of the skin around the device were statistically analyzed for both groups.

[0076] As a result, Figure 3As shown, the commercially available sweat detection device exhibits numerous noticeable air bubbles around its perimeter, likely due to moisture retention of sweat in that area. In contrast, the body fluid-based biological detection device of Example 1 shows no obvious air bubbles. Furthermore, microscopic observation of the filter layer of the body fluid-based biological detection device of Example 1 yields the following results: Figure 4 As shown, the filter layer has numerous pores, which may increase water evaporation. Furthermore, it significantly reduces interference from sweat in other areas (non-detection areas), ensuring the cleanliness of the surrounding environment. In addition, the experimental group obtained biological signals in a shorter time and with more signals than the control group. This is likely because the filter layer in the experimental group filtered out most of the water from the sweat, resulting in a higher concentration of biomarkers in the detected samples. Moreover, the molecularly imprinted polymer layer in the experimental group can accurately identify biomarkers.

[0077] Example 3 Skin affinity test

[0078] The material for preparing the filter layer of the body fluid-based biological detection device of Example 1 was applied to the skin surface of the subject, and the skin surface condition was observed for a period of time, and the subject's feelings were recorded.

[0079] The results are as follows Figure 5 As shown, the subjects' skin showed no redness, swelling, or rashes, and the subjects also experienced no discomfort.

[0080] Example 4

[0081] This embodiment uses the body fluid-based biological detection device from Example 1 to detect and analyze sweat.

[0082] like Figure 6 , 7 As shown, the main steps include:

[0083] Step 1: The first step in analyzing any biofluid (body fluid) is to correctly collect the test sample from the subject's body. This is crucial because it is difficult to accurately quantify biomarkers without proper sample collection. In particular, sweat contains 99% water and only 1% biomarkers, making proper collection even more important. Therefore, we used a highly porous and breathable material (UHMWPE membrane filtration layer) that allows for rapid evaporation of moisture, retaining only biomarkers within the ultra-high molecular weight polyethylene membrane.

[0084] Step 2: However, the desired biomarkers may only represent a small fraction of the total biomarkers (e.g., 0.1%), so we need deeper molecular recognition capabilities. In our case, we incorporated a molecularly imprinted polymer (MIP) that can specifically bind to the desired molecules, which are then linked to the organic electrochemical transistor (OECT) of the biochemical sensor.

[0085] Step 3: The biological signal is converted into an electrical signal and flows in the OECT, where the biochemical sensor detects and analyzes the electrical signal;

[0086] Step 4: In addition, in order to capture the amount of sweat collected for calibration for each user, we use an MXene-based sensor to measure humidity. This sensor correlates resistance changes with the collected sweat vapor so that we can combine the collected biomarkers with the volume to obtain accurate sweat biomarker concentrations.

[0087] Step 5: Finally, after achieving real-time tracking of biomarkers, a software system was developed that can process and build personalized algorithms to provide individualized measurements for each person.

[0088] Example 5

[0089] This embodiment uses the body fluid-based biological detection device from Example 1 to detect and analyze sweat and sweat vapor.

[0090] like Figure 6 , 7 As shown, the main steps include:

[0091] Step 1: The first step in analyzing any biofluid (body fluid) is to correctly collect the test sample from the subject's body. This is crucial because it is difficult to accurately quantify biomarkers without proper sample collection. In particular, sweat contains 99% water and only 1% biomarkers, making proper collection even more important. Therefore, we used a highly porous and breathable material (UHMWPE membrane filtration layer) that allows for rapid evaporation of moisture, retaining only biomarkers within the ultra-high molecular weight polyethylene membrane.

[0092] Step 2: However, the desired biomarkers may only represent a small fraction of the total biomarkers (e.g., 0.1%), so we need deeper molecular recognition capabilities. In our case, we incorporated a molecularly imprinted polymer (MIP) that can specifically bind to the desired molecules, which are then linked to the organic electrochemical transistor (OECT) of the biochemical sensor.

[0093] Step 3: The biological signal is converted into an electrical signal and flows in the OECT, where the biochemical sensor detects and analyzes the electrical signal;

[0094] Step 4: In addition, in order to capture the amount of sweat collected for calibration for each user, we use an MXene-based sensor to measure humidity. This sensor correlates resistance changes with the collected sweat vapor so that we can combine the collected biomarkers with the volume to obtain accurate sweat biomarker concentrations.

[0095] Step 5: Finally, after achieving real-time tracking of biomarkers, a software system was developed that can process and build personalized algorithms to provide individualized measurements for each person.

[0096] Step Six: Use the methods from Steps One to Five to detect biomarkers in the subject's unconscious perspiration (sweat vapor);

[0097] Step 7: Compare and analyze the data obtained in Step 5 with the data obtained in Step 6.

[0098] The applicant declares that, in the process of describing the above-mentioned specification:

[0099] The terms "this embodiment," "an embodiment of the present invention," "as shown," "further," and "further improved technical solutions," etc., indicate that the specific features, structures, materials, or characteristics described in the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms are not necessarily directed at the same embodiment or example, and the specific features, structures, materials, or characteristics described can be combined or combined in any suitable manner in one or more embodiments or examples. Furthermore, without causing contradiction, those skilled in the art can combine or combine the different embodiments or examples described in this specification and the features of the different embodiments or examples.

[0100] Finally, it should be noted that:

[0101] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them;

[0102] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention. Non-essential improvements, adjustments or substitutions made by those skilled in the art based on the content of this specification are all within the scope of protection claimed by the present invention.

Claims

1. A biological testing device based on a body fluid, characterized by, It comprises, from bottom to top in sequence, a water filtering layer, a molecularly imprinted polymer layer and a flexible circuit board layer, wherein the flexible circuit board layer integrates a biochemical sensor and a humidity sensor; The water filtering layer comprises a UHMWPE film; The water filtering layer filters water in the body fluid, the molecularly imprinted polymer layer recognizes and binds biomarkers in the filtered body fluid, and the binding of biomarkers to the molecularly imprinted polymer layer causes the current of the biochemical sensor to change, thereby converting the biological signal in the body fluid into a physical signal; The humidity sensor monitors the humidity around the detection device and transmits the humidity information to the biochemical sensor in real time, and the biochemical sensor takes the humidity data as the volume parameter of the body fluid and combines the data of biomarkers transmitted by the molecularly imprinted polymer layer to analyze and calculate the concentration of biomarkers; The biochemical sensor comprises an organic electrochemical transistor-based biochemical sensor; The body fluid comprises sweat and sweat vapor.

2. A bodily fluid based biological testing device as claimed in claim 1, wherein, The humidity sensor comprises an MXene-based humidity sensor.

3. The bodily fluid-based biological testing device of claim 1, wherein, It also comprises a smart terminal connected to the flexible circuit board layer through network signals.

4. The bodily fluid-based biological testing device of claim 1, wherein, The detection device is wearable or adhesive.

5. The bodily fluid-based biological testing device of claim 1, wherein, The biomarkers include biological metabolites, hormones, electrolytes and proteins.

6. A method for biological detection based on a body fluid using the biological detection device based on a body fluid according to any one of claims 1 to 5, characterized by, It comprises the following steps: S1, filtering water in the body fluid through the water filtering layer; S2, the molecularly imprinted polymer layer recognizes and binds biomarkers in the filtered body fluid, and the body fluid comprises sweat and sweat vapor; S3, an organic electrochemical transistor-based biochemical sensor is used, and the binding of biomarkers to the molecularly imprinted polymer layer causes the current of the biochemical sensor to change, thereby converting the biological signal in the body fluid into a physical signal; S4, monitoring the humidity around the detection device and transmitting the humidity information to the biochemical sensor in real time, and the biochemical sensor takes the humidity data as the volume parameter of the body fluid and combines the data of biomarkers transmitted by the molecularly imprinted polymer layer to analyze and calculate the concentration of biomarkers, and uses the detection results of biomarkers of sweat vapor under the condition of unconscious perspiration to correct the detection results of biomarkers of sweat of the subject.

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