Use of furanone in preparing products for improving animal rumen fermentation

Abstract

The invention belongs to the technical field of animal breeding, and specifically relates to a use of furanone in preparing a product for improving animal rumen fermentation. Experiments have shown that furanone can improve rumen fermentation, thereby improving the feed utilization efficiency of ruminants.

Description

Technical Field

[0001] The invention belongs to the technical field of animal breeding, and particularly relates to use of furanone in preparing a product for improving animal rumen fermentation. Background Art

[0002] Feed expenditure accounts for more than 60% of animal production costs, and feed efficiency is the core proposition of ruminant production efficiency. Improving feed efficiency can be done from three aspects: breed selection, nutritional regulation and environmental optimization. Among them, nutritional regulation is widely used because of its simple operation, short cycle and high cost performance.

[0003] At present, the measures for nutritional regulation mainly include optimizing the types and composition ratios of feed raw materials and feed additives, among which feed additives are widely used. There are nutritional additives and non-nutritional additives. The former focuses on the digestion, absorption and utilization of the additives themselves in the animal intestine, while the latter mainly promotes the utilization efficiency of nutrients in the feed. Due to the presence of the rumen, ruminants' digestion and metabolism of nutrients in the feed are significantly different from those of other animals. A notable feature is the presence of ruminant rumen microorganisms. Rumen microorganisms degrade and ferment the nutrients in the feed in the rumen to produce volatile fatty acids, microbial proteins and other substances required for the maintenance and production of ruminants. In general, the more of the above substances synthesized, the higher the utilization efficiency of the feed by ruminants. To a certain extent, the optimization of rumen fermentation parameters can improve the feed efficiency of ruminants. Therefore, improving rumen fermentation by regulating rumen microorganisms is a new idea to improve the feed efficiency of ruminants.

[0004] Quorum sensing is a communication mechanism in which bacteria use signal molecules to communicate within and between species. With the help of this communication mechanism, microorganisms can achieve physiological functions and behavioral regulation in the form of a group that cannot be completed by individual microorganisms, such as the formation of biofilms and bioluminescence. Rumen microorganisms have high bacterial diversity and rich species content. Regulating rumen bacteria can improve rumen fermentation in ruminants and thus improve the efficiency of feed utilization. It is currently known that biofilms will form during the degradation of feed in the rumen, and quorum sensing can regulate the formation of biofilms. However, there is currently no case of using quorum sensing theory to improve rumen fermentation, let alone substances that improve feed efficiency developed based on quorum sensing. Summary of the invention

[0005] In view of the above technical problems, the present invention provides a use of furanone in preparing a product for improving animal rumen fermentation.

[0006] The specific technical solutions provided by the present invention are as follows:

[0007] In a first aspect, the present invention provides a use of furanone in preparing a product for improving animal rumen fermentation. The animal described in the present invention is a ruminant.

[0008] Preferably, the furanone is used to improve the utilization efficiency of feed by animals.

[0009] Preferably, the furanone is used at a concentration of 150 to 1200 μg / mL.

[0010] Preferably, the animal is a cow.

[0011] The second aspect of the present invention provides a product for improving animal rumen fermentation, which contains the furanone as a main active ingredient.

[0012] Preferably, the product is a medicine.

[0013] Preferably, the product includes veterinary acceptable adjuvants or carriers. The product is prepared into veterinary acceptable preparations, such as granules, powders or liquid preparations, and suitable adjuvants or carriers, such as diluents, wetting agents, adhesives, regulators, solubilizers, cosolvents, preservatives, etc., may be added during the preparation. Exemplarily, the diluent is selected from starch, sucrose, cellulose, inorganic salts, etc.; the wetting agent is selected from water, ethanol, etc.; the binder is selected from starch slurry, dextrin, sugar, cellulose derivatives, gelatin, povidone, polyethylene glycol, etc.; the regulator is selected from pigments, flavors, sweeteners, mucilages, deodorants, etc.; the solubilizer is selected from Tweens, polyoxyethylene fatty alcohol ethers, soaps, sulfates, sulfonates, etc.; the cosolvent is selected from organic acids and salts thereof, amide and amine compounds, inorganic salts, polyethylene glycol, glycerol, etc.; the preservative is selected from parabens, organic acids and salts thereof, quaternary ammonium compounds, chlorhexidine acetate, alcohols, phenols, and volatile oils, etc.

[0014] Preferably, the product is a feed additive.

[0015] Preferably, the furanone is mixed with conventional feed, such as at least one of corn, soybean meal, wheat bran, and wheat straw, or fermentation products of these substances, or any ruminant feed sold on the market.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] The present invention provides a use of furanone in preparing a product for improving animal rumen fermentation. Experiments have shown that furanone can improve rumen fermentation, thereby improving the feed efficiency of ruminants.

[0018] Although existing feed additives can improve rumen fermentation, they also affect the diversity and species richness of rumen microorganisms. Some additives are also prone to drug resistance in microorganisms during long-term use. The furanone provided by the present invention is a substance developed based on regulating the self-communication mechanism of microorganisms. It will not affect the diversity and species richness of rumen microorganisms, and will not produce drug resistance after long-term use. DETAILED DESCRIPTION

[0019] The following is a detailed description of an embodiment of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0020] The present invention provides the use of furanone in preparing a product for improving animal rumen fermentation. This property of furanone is developed by the present invention based on quorum sensing, and it will not affect the diversity and richness of microorganisms, nor will it produce drug resistance. It has been proved by experiments that furanone can improve rumen fermentation, thereby improving the feed utilization efficiency of ruminants.

[0021] The product can be a medicine or a feed additive. The medicine is a veterinary acceptable dosage form, such as granules, powders or liquid preparations. In the specific preparation, permitted excipients or carriers can be added so that the preparation can be better absorbed by animals or convenient for transportation and storage of the preparation. Furanone can also be mixed with conventional feeds, such as corn, soybean meal, bran, etc., when used in specific applications, or it can be a fermentation product of these substances.

[0022] The chemical formula of furanone used in the present invention is C6H8O3, and the structural formula is shown in Formula I:

[0023]

[0024] The effect of furanone in improving rumen fermentation in ruminants is described below.

[0025] 1. Test process

[0026] 1.1. Collection of rumen fluid

[0027] The rumen contents of 4 dairy cows were collected by oral intubation, and the rumen fluid was obtained after being mixed evenly and passed through four layers of gauze.

[0028] 1.2 Preparation of culture medium

[0029] The preparation of artificial saliva refers to the conventional method, and the culture medium is composed of rumen fluid and artificial saliva mixed evenly in a volume ratio of 1:2.

[0030] The mass composition of the in vitro fermentation substrate is as follows: 20.00% corn, 19.80% soybean meal, 3.49% wheat bran, 50.71% wheat straw, 1.00% calcium hydrogen phosphate, 0.50% stone powder, 0.50% salt, and 4.00% premix. Furanone was added to the fermentation substrate at a dose of 0, 150, 300, 600, and 1200 μg / mL, with 5 replicates for each dose. The fermentation was terminated in an ice bath after 48 hours of fermentation in a culture medium composed of rumen fluid and artificial saliva under anaerobic conditions.

[0031] 1.3 Sample collection

[0032] The fermentation liquid was passed through four layers of gauze and dispensed into centrifuge tubes and stored at -80°C until testing.

[0033] 2. Index detection

[0034] The pH value of the fermentation broth was measured with a portable pH meter immediately after filtration. Each sample was measured 3 times, and the average value was taken as the representative value of the fermentation broth.

[0035] Ammonia nitrogen (NH3-N) was determined by phenol-sodium hypochlorite colorimetric method.

[0036] Volatile fatty acids were analyzed by gas chromatography using the peak area external standard method to determine various volatile fatty acids and their corresponding contents based on relative retention times.

[0037] The signal molecule AI-2 was determined by Fe(III)-1,10-phenanthroline colorimetric method.

[0038] The biofilm content was determined by crystal violet staining.

[0039] 3. Statistical analysis

[0040] All data were analyzed by one-way analysis of variance, with P < 0.05 being considered significant. Tukey's multiple comparisons between doses were performed for indicators with significant differences.

[0041] 4. Test results

[0042] The results are shown in Table 1.

[0043] Table 1 Test results of various indicators in fermentation broth

[0044]

[0045]

[0046] Table Notes: No lowercase letters or the same lowercase letters in the table indicate no significant difference, while different lowercase letters indicate significant difference. SEM is the standard error of the mean. 0, 150, 300, 600, 1200 indicate that the dosage of furanone added is 0 μg / mL, 150 μg / mL, 300 μg / mL, 600 μg / mL, 1200 μg / mL, respectively.

[0047] According to the test results, the addition of furanone can increase the pH value to a certain extent, reduce the concentration of NH3-N, use ammonia nitrogen to generate more microbial protein in a more suitable rumen environment, and synthesize more volatile fatty acids, which is manifested in the numerical increase of acetic acid, propionic acid, butyric acid and total volatile fatty acid content. The addition of 300μg / mL has the best effect on improving rumen fermentation. The addition of 300μg / mL increases the content of acetic acid, propionic acid, butyric acid and total volatile fatty acids by 6.76%, 29.93%, 11.99% and 14.25%, respectively.

[0048] In addition, the optimization of acetate, propionate, butyrate, and total volatile fatty acid contents was associated with increased AI-2 signaling molecule concentrations and biofilm content, further demonstrating that furanone can optimize rumen fermentation.

[0049] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A use of furanone in preparing a product for improving animal rumen fermentation, characterized in that: The furanone is used for increasing the acetic acid content, propionic acid content, butyric acid content, total volatile fatty acid content, pH value or reducing NH3-N concentration in rumen fermentation liquid.

2. The use according to claim 1, characterized in that The furanone is used for improving the utilization efficiency of feed by animals.

3. The use according to claim 1, characterized in that The furanone is used at a concentration of 150 to 1200 μg / mL.

4. The use according to claim 1, characterized in that The animal is a dairy cow.

5. A product for improving animal rumen fermentation, characterized in that: The furanone described in claim 1 is used as a main active ingredient.

6. The product according to claim 5, characterized in that The product described is a drug.

7. The product according to claim 6, characterized in that The product includes veterinary acceptable excipients or carriers.

8. The product according to claim 5, characterized in that The product is a feed additive.

9. The product according to claim 8, characterized in that The furanone is mixed with feed for use.

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