A whitening, anti-aging and wrinkle-lightening composition and preparation method thereof

Abstract

The present invention relates to a composition for whitening, anti-aging and wrinkle fading and a preparation method thereof, and belongs to the technical field of cosmetics. The composition contains extract components such as bamboo fungus, charcoal motherwort, yellow catfish, gooseberry, and blueberry; wherein the bamboo fungus extract is obtained by extracting protein from bamboo fungus and then using papain and subtilisin for graded enzymolysis; the charcoal motherwort extract is obtained by taking charcoal motherwort for hemicellulase enzymolysis, fermenting with Bacillus subtilis, and finally enzymolysis with trypsin; the yellow catfish extract is obtained by fermenting yellow catfish with Propionibacterium scheffleri and Clostridium butyricum and then enzymolysis with trypsin; the gooseberry extract is obtained by enzymolysis of gooseberry with pepsin; and the blueberry extract is obtained by graded enzymolysis of blueberry with pepsin and trypsin. The compound use of each component has a good synergistic effect, and can realize fast and efficient skin brightening, whitening, repairing skin elasticity, fading wrinkles, delaying aging, and is safe and non-irritating.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a whitening, anti-aging and wrinkle-lightening composition and a preparation method thereof. Background Art

[0002] Damaged or aged facial skin cells will show allergies, redness, dull yellow, spots, acne, wrinkles and other phenomena, which greatly affect the facial image. Therefore, in order to improve skin health and delay the aging process, modern people cannot do without cosmetics for daily skin care. There are many kinds of functional cosmetics, which are mainly divided into: moisturizing, anti-allergic, whitening, spot removal, acne removal, and wrinkle removal. Its active ingredients are often added with various plant extracts and / or various animal extracts, and hormones, lead, mercury and other ingredients are also used. For example, CN102302440B discloses a Chinese herbal medicine skin rejuvenation, whitening, anti-aging, wrinkle removal, and spot removal cosmetic composition, in which Chinese herbal medicine extracts include saffron, ganoderma lucidum, atractylodes, white peony root, white poria, white ampel, licorice, qinpi, bletilla striata, pine needles and mint extracts; various extracts have antioxidant effects, can improve skin metabolism, promote skin blood circulation, increase skin nutrition, remove wrinkles and spots on the skin, and make the skin more delicate, smooth, white and elastic. For example, CN104825804B discloses a whitening, moisturizing and anti-aging composition and its preparation method and use, the composition ingredients include Bletilla striata, Morus alba bark, Radix Pseudostellariae, Periostracum Cicadae, Lithospermum officinale, etc.; each ingredient has good inhibitory activity on tyrosinase, strong moisturizing effect, long duration, can effectively enhance skin elasticity, and synergistically enhances the activity of inhibiting melanin production. For example, CN110269822B discloses a salmon proteoglycan moisturizing skin care lotion and its preparation method and use, the ingredients include liquid paraffin, caprylic triglyceride, coconut oil, carbomer, polyglutamic acid, butylene glycol, glycerol, xanthan gum, salmon proteoglycan, Bacillus nuclease, honeysuckle dew, surfactant, pH regulator, etc., wherein salmon proteoglycan, honeysuckle dew, and polyglutamic acid are reasonably formulated to produce a pure natural natural skin care moisturizing lotion without heavy metal addition and side effects, which effectively improves skin metabolism, helps to moisturize and whiten the skin, improves skin elasticity, and removes wrinkles.

[0003] In modern cosmetics, animal and plant extracts are used for whitening, anti-aging and wrinkle removal. Although they are safer, less prone to allergies and less likely to damage the skin than cosmetics containing hormones, lead, mercury and other ingredients, the whitening and wrinkle removal effects of various animal and plant extracts are still not ideal and cannot meet people's increasingly high demand for cosmetic effects. Therefore, it is necessary to continuously develop and improve technology, shorten the course of whitening and wrinkle removal treatments, maintain beauty for a long time, and effectively delay the aging process. Summary of the invention

[0004] The whitening and wrinkle-removing effects of the existing active ingredients of animal and plant extracts are not ideal, that is, the whitening and wrinkle-removing effects are not obvious in the short term, and the beauty state cannot be maintained for a long time after long-term use, and the degree of aging delay is low. The present invention provides a whitening, anti-aging and wrinkle-removing composition and a preparation method thereof. The composition contains bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract, blueberry extract, resveratrol, niacinamide, lecithin and other ingredients, wherein the bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are prepared by special graded enzymatic hydrolysis or fermentation to obtain small molecule extracts below 2kDa, and the compound use of each component has a good synergistic effect, which can achieve fast and efficient skin brightening, whitening, repair skin elasticity, reduce wrinkles, and delay aging; and it is safe and non-irritating. The specific technical scheme is as follows:

[0005] A composition for whitening, anti-aging and wrinkle reduction is prepared from the following raw materials in parts by mass: 0.5 to 3 parts of bamboo fungus extract, 0.5 to 1.5 parts of charcoalwort extract, 1 to 2.5 parts of yellow catfish extract, 0.3 to 0.6 parts of gooseberry extract, 0.3 to 0.6 parts of blueberry extract, 0.05 to 0.3 parts of resveratrol, 0.05 to 0.5 parts of niacinamide, 0.5 to 2 parts of lecithin, 0.5 to 2 parts of carbomer and 50 to 60 parts of purified water;

[0006] The bamboo fungus extract is obtained by extracting protein from the bamboo fungus, and then using papain and subtilisin for graded enzymatic hydrolysis to obtain an extract with a size below 2kDa; the pyrochelidon extract is obtained by hydrolyzing pyrochelidon with hemicellulase, fermenting with Bacillus subtilis, and finally hydrolyzing with trypsin to obtain an extract with a size below 2kDa; the yellow catfish extract is obtained by fermenting yellow catfish with Propionibacterium scheffleri and Clostridium butyricum, and then hydrolyzing with trypsin to obtain an extract with a size below 2kDa; the gooseberry extract is obtained by hydrolyzing gooseberry with pepsin to obtain an extract with a size below 2kDa; and the blueberry extract is obtained by hydrolyzing blueberry with pepsin and trypsin for graded enzymatic hydrolysis to obtain an extract with a size below 2kDa.

[0007] In the above technical scheme, the preparation method of the bamboo fungus extract is: taking fresh bamboo fungus to break the spores and crushing them, adding 0.15mol / L~0.20mol / L saline, soaking them for 1h~2h by 30KHz~40KHz ultrasound, centrifuging, taking the supernatant, adding 4mol / L~4.5mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 8 to 10 times that of the protein precipitate, adjusting the pH to 7.0 to pH 7.5, adding papain, enzymolysis at 40℃~45℃ for 1h~1.5h, inactivating the enzyme in a water bath, cooling to 30℃~40℃, adding subtilisin, enzymolysis for 1.5h~2h, inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray drying, and obtaining a bamboo fungus extract.

[0008] The amount of the salt water added is 8 to 10 times the mass of the bamboo fungus; the amount of the papain added is 0.3% to 1% of the mass of the protein precipitation; the amount of the subtilisin added is 0.2% to 0.6% of the mass of the protein precipitation; the temperature of the water bath enzyme inactivation is 85°C to 95°C, and the time is 30min to 40min.

[0009] In the above technical scheme, the preparation method of the charcoal motherwort extract is: grind the charcoal motherwort, add water 8 to 10 times the mass of the charcoal motherwort, adjust the pH to 4.0 to pH 5.5, add hemicellulase, enzymolysis at 50°C to 60°C for 1.5h to 2h, inactivate the enzyme in a water bath, reduce the temperature to 35°C to 40°C, adjust the pH to 6.8 to pH 7.4, add Bacillus subtilis, ferment at 35°C to 40°C for 48h to 72h, sterilize in a water bath, reduce the temperature to 36°C to 38°C, adjust the pH to 7.8 to pH 8.5, add trypsin, enzymolysis for 1.5h to 2h, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray dry, and obtain the charcoal motherwort extract.

[0010] The amount of hemicellulase added is 1% to 3% of the mass of charcoal grass; the amount of Bacillus subtilis added is 0.5% to 3% of the mass of charcoal grass; the amount of trypsin added is 0.5% to 1% of the mass of charcoal grass; the temperature of the water bath enzyme inactivation is 85°C to 95°C, and the time is 30min to 40min; the temperature of the water bath sterilization is 85°C to 100°C, and the time is 30min to 40min.

[0011] In the above technical scheme, the preparation method of the yellow catfish extract is: take yellow catfish, remove the internal organs, crush it to obtain fish paste, add water 10 to 12 times the mass of the fish paste to the fish paste, add Propionibacterium schreiberis and Clostridium butyricum, ferment for 12 hours to 15 hours, sterilize in a water bath, reduce the temperature to 36°C to 38°C, adjust the pH to 7.8 to pH 8.5, add trypsin, enzymolysis for 1.5 hours to 2 hours, then inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane, obtain liquid A below 2kDa, freeze-dry, and obtain the yellow catfish extract.

[0012] The amount of Propionibacterium schizophylla added is 1% to 3% of the mass of the fish paste; the amount of Clostridium butyricum added is 0.5% to 2% of the mass of the fish paste; the amount of trypsin added is 0.5% to 1% of the mass of the fish paste; the temperature of the water bath sterilization is 85°C to 100°C, and the time is 30min to 40min; the temperature of the water bath enzyme inactivation is 85°C to 95°C, and the time is 30min to 40min.

[0013] In the above technical scheme, the preparation method of the gooseberry extract is: crushing the gooseberries, adding water 5 to 6 times the mass of the gooseberries, adjusting the pH to 1.5 to pH 2.0, adding pepsin 0.2% to 0.5% by mass of the gooseberries, hydrolyzing at 36°C to 38°C for 1h to 2h, inactivating the enzyme in a water bath at 85°C to 95°C for 30min to 40min, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane, taking the filtrate less than 2kDa, spray drying, and obtaining the gooseberry extract.

[0014] In the above technical scheme, the preparation method of the blueberry extract is as follows: blueberries are crushed, water is added that is 5 to 6 times the mass of blueberries, the pH is adjusted to 1.5 to pH 2.0, pepsin is added that is 0.2% to 0.5% by mass of blueberries, and after enzymatic hydrolysis at 36°C to 38°C for 1h to 2h, the enzyme is inactivated in a water bath at 85°C to 95°C for 30min to 40min, and after the pH is reduced to 36°C to 38°C, the pH is adjusted to 7.8 to pH 8.5, trypsin is added that is 0.2% to 0.5% by mass of blueberries, the enzyme is inactivated in a water bath at 85°C to 95°C for 30min to 40min, and after the pH is reduced to room temperature, a 2kDa ultrafiltration membrane is used for filtration to obtain a liquid A below 2kDa, and the liquid is spray-dried to obtain a blueberry extract.

[0015] The preparation method of the above-mentioned whitening, anti-aging and wrinkle-reducing composition comprises the following steps:

[0016] According to the mass fraction, the purified water is heated to 75°C-80°C, carbomer and niacinamide are added, mixed evenly, cooled to 50°C-60°C, resveratrol and lecithin are added, mixed evenly, cooled to 35°C-40°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, cooled to obtain a composition.

[0017] The present invention provides a whitening, anti-aging and wrinkle-lightening composition and a preparation method thereof, which, compared with the prior art, has the following beneficial effects:

[0018] 1. The composition of the present invention contains bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract, blueberry extract, resveratrol, niacinamide, lecithin, carbomer and other ingredients. The combined use of the ingredients has a good synergistic effect, and can quickly and efficiently brighten the skin, whiten the skin, repair skin elasticity, reduce wrinkles, and delay aging.

[0019] Second, the bamboo fungus spore component has anti-inflammatory and whitening effects. The bamboo fungus extract of the present invention is obtained by extracting protein from bamboo fungus, and then using papain and subtilisin for graded enzymolysis to obtain an extract below 2kDa; it has a good effect of inhibiting tyrosinase activity and inhibiting melanin precipitation, and promotes synergistic whitening significantly; it has a good effect of scavenging hydroxyl free radicals, and can greatly improve the antioxidant and anti-aging abilities of cells.

[0020] 3. The charcoal grass has the effects of anti-inflammatory, anti-oxidation, improving blood circulation, removing dampness and detoxification, and improving immunity. The charcoal grass extract of the present invention is obtained by hydrolyzing the charcoal grass with hemicellulase, fermenting with Bacillus subtilis, and finally hydrolyzing with trypsin to obtain an extract below 2kDa; it has a good effect of promoting the removal of hydroxyl free radicals, can greatly improve the antioxidant and anti-aging abilities of cells, and improve cell vitality.

[0021] 4. The Pelteobagrus vachelli extract of the present invention is an extract below 2 kDa obtained by fermenting Pelteobagrus vachelli with Propionibacterium shermanii and Clostridium butyricum and then hydrolyzing with trypsin; it has excellent effects of improving skin elasticity and roughness. The Pelteobagrus vachelli extract contains rich small molecule peptides, which can promote cells to produce collagen, and cooperate with other ingredients to restore cell vitality, improve skin condition, and make the skin smooth, delicate and elastic.

[0022] 5. Gooseberry has the effect of enhancing cellular immunity, preventing bacterial and viral invasion and allergic symptoms, promoting blood circulation and promoting blood circulation, and anti-oxidation. The gooseberry extract of the present invention is an extract below 2kDa obtained by enzymolysis of gooseberry with pepsin; it can greatly improve the anti-aging performance of gooseberry components, contains rich gooseberry small molecule peptides, and cooperates with other small molecule active substances to enhance synergy, can well inhibit tyrosinase, inhibit melanin precipitation, promote whitening effect, promote and improve the removal of hydroxyl free radicals, and improve the antioxidant and anti-aging ability of cells; make the skin smooth and delicate.

[0023] Sixth, blueberry is used in cosmetics to have the effects of anti-oxidation, anti-inflammatory, soothing, skin brightening, pore shrinking, anti-acne, moisturizing, etc. The blueberry extract of the present invention is an extract below 2kDa obtained after blueberry is graded enzymatically hydrolyzed by pepsin and trypsin, which contains rich small molecule peptides and other active ingredients, is easy to absorb, and can have more efficient whitening and brightening, inhibit tyrosinase, inhibit melanin precipitation, anti-oxidation, and anti-wrinkle effects when used in combination.

[0024] 7. After trial testing, the bamboo fungus extract, the charcoal motherwort extract and the yellow catfish extract of the present invention have a synergistic effect. When used in combination, the synergistic effect of scavenging hydroxyl free radicals is very prominent, and can greatly improve the effects of whitening, restoring skin elasticity, restoring skin tenderness, and reducing wrinkles.

[0025] In summary, the combined use of the ingredients such as the bamboo fungus extract, the charcoal motherwort extract, the yellow catfish extract, the gooseberry extract, the blueberry extract, the resveratrol, the niacinamide, the lecithin and the like in the composition of the present invention has a good synergistic effect, and the effect is obvious after 2 months of use, and can achieve fast and efficient skin brightening, whitening, repairing skin elasticity, fading wrinkles, and delaying aging; and has good practical value. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0027] Example 1

[0028] A composition for whitening, anti-aging and wrinkle reduction is prepared from the following raw materials in parts by mass: 2 parts of bamboo fungus extract, 1 part of pyrocaul extract, 1.5 parts of yellow catfish extract, 0.5 parts of gooseberry extract, 0.5 parts of blueberry extract, 0.2 parts of resveratrol, 0.3 parts of niacinamide, 1 part of lecithin, 1.5 parts of carbomer and 55 parts of purified water.

[0029] In the above technical scheme, the preparation method of the bamboo fungus extract is: taking fresh bamboo fungus to break the spores and crushing them, adding 0.15 mol / L saline, soaking them for 1.5 hours by 35KHz ultrasound, centrifuging, taking the supernatant, adding 4 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water with a mass of 9 times that of the protein precipitate, adjusting the pH to 7.2, adding papain, enzymolysis at 42°C for 1 hour, inactivating the enzyme in a water bath, cooling to 35°C, adding subtilisin, enzymolysis for 1.5 hours, inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray drying, and obtaining a bamboo fungus extract.

[0030] The amount of salt water added is 9 times the mass of bamboo fungus; the amount of papain added is 0.5% of the mass of protein precipitation; the amount of subtilisin added is 0.4% of the mass of protein precipitation; the temperature of water bath enzyme inactivation is 90°C and the time is 35 minutes.

[0031] In the above technical scheme, the preparation method of the charcoal motherwort extract is: grind the charcoal motherwort, add 9 times the mass of water of the charcoal motherwort, adjust the pH to 5.0, add hemicellulase, enzymolysis at 55°C for 1.5 hours, inactivate the enzyme in a water bath, reduce the temperature to 37°C, adjust the pH to 7.0, add Bacillus subtilis, ferment at 37°C for 60 hours, sterilize in a water bath, reduce the temperature to 37°C, adjust the pH to 8.0, add trypsin, enzymolysis for 1.5 hours, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray dry, and obtain the charcoal motherwort extract.

[0032] The amount of hemicellulase added is 2% of the mass of pyrocalyx; the amount of Bacillus subtilis added is 1.5% of the mass of pyrocalyx; the amount of trypsin added is 0.6% of the mass of pyrocalyx; the temperature of the water bath enzyme inactivation is 90°C and the time is 35 minutes; the temperature of the water bath sterilization is 90°C and the time is 35 minutes.

[0033] In the above technical scheme, the preparation method of the yellow catfish extract is: take yellow catfish, remove the internal organs, crush it to obtain fish paste, add water 11 times the mass of the fish paste to the fish paste, add Propionibacterium schreiberis and Clostridium butyricum, ferment for 14 hours, sterilize in a water bath, reduce to 37°C, adjust the pH to 8.0, add trypsin, enzymolysis for 1.5 hours, then inactivate the enzyme in a water bath, reduce to room temperature, filter with a 2kDa ultrafiltration membrane, obtain liquid A below 2kDa, freeze-dry, and obtain the yellow catfish extract.

[0034] The amount of Propionibacterium schizophylla added is 2% of the mass of the fish paste; the amount of Clostridium butyricum added is 2% of the mass of the fish paste; the amount of trypsin added is 0.6% of the mass of the fish paste; the temperature of the water bath sterilization is 90°C and the time is 35 minutes; the temperature of the water bath enzyme inactivation is 90°C and the time is 35 minutes.

[0035] In the above technical scheme, the preparation method of the gooseberry extract is: crush the gooseberries, add 5.5 times the mass of water of the gooseberries, adjust the pH to 1.8, add 0.3% of the mass of pepsin of the gooseberries, hydrolyze at 37°C for 1.5h, inactivate the enzyme in a 90°C water bath for 35min, cool to room temperature, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, spray dry, and obtain the gooseberry extract.

[0036] In the above technical scheme, the preparation method of the blueberry extract is: crushing the blueberries, adding 5.5 times the mass of water of the blueberries, adjusting the pH to 1.8, adding 0.3% of the mass of pepsin of the blueberries, enzymolysis at 37°C for 1.5 hours, inactivating the enzyme in a water bath at 90°C for 35 minutes, cooling to 37°C, adjusting the pH to 8.0, adding 0.3% of the mass of trypsin of the blueberries, enzymolysis for 1.5 hours, inactivating the enzyme in a water bath at 90°C for 35 minutes, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane, obtaining a liquid A below 2kDa, spray drying, and obtaining a blueberry extract.

[0037] The preparation method of the above-mentioned whitening, anti-aging and wrinkle-reducing composition comprises the following steps:

[0038] According to the mass fraction, the purified water is heated to 78°C, carbomer and niacinamide are added, mixed evenly, cooled to 55°C, resveratrol and lecithin are added, mixed evenly, cooled to 38°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, cooled to obtain a composition.

[0039] Example 2

[0040] A composition for whitening, anti-aging and wrinkle reduction is prepared from the following raw materials in parts by mass: 0.5 parts of bamboo fungus extract, 0.5 parts of pygmy caltrop extract, 1 part of yellow catfish extract, 0.3 parts of gooseberry extract, 0.3 parts of blueberry extract, 0.05 parts of resveratrol, 0.05 parts of niacinamide, 0.5 parts of lecithin, 0.5 parts of carbomer and 50 parts of purified water.

[0041] In the above technical scheme, the preparation method of the bamboo fungus extract is: taking fresh bamboo fungus to break the spores and crushing them, adding 0.15 mol / L saline, soaking them for 1 hour using 30KHz ultrasound, centrifuging, taking the supernatant, adding 4 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water with a mass 8 times that of the protein precipitate, adjusting the pH to 7.0, adding papain, enzymolysis at 40°C for 1 hour, inactivating the enzyme in a water bath, cooling to 30°C, adding subtilisin, enzymolysis for 1.5 hours, inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray drying, and obtaining a bamboo fungus extract.

[0042] The amount of salt water added is 8 times the mass of bamboo fungus; the amount of papain added is 0.3% of the mass of protein precipitation; the amount of subtilisin added is 0.2% of the mass of protein precipitation; the temperature of water bath enzyme inactivation is 85°C and the time is 30 minutes.

[0043] In the above technical scheme, the preparation method of the charcoal motherwort extract is: grind the charcoal motherwort, add water 8 times the mass of the charcoal motherwort, adjust the pH to 4.0, add hemicellulase, enzymolysis at 50°C for 1.5 hours, inactivate the enzyme in a water bath, reduce the temperature to 35°C, adjust the pH to 6.8, add Bacillus subtilis, ferment at 35°C for 48 hours, sterilize in a water bath, reduce the temperature to 36°C, adjust the pH to 7.8, add trypsin, enzymolysis for 1.5 hours, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray dry, and obtain the charcoal motherwort extract.

[0044] The amount of hemicellulase added is 1% of the mass of pyrocalyx; the amount of Bacillus subtilis added is 0.5% of the mass of pyrocalyx; the amount of trypsin added is 0.5% of the mass of pyrocalyx; the temperature of the water bath enzyme inactivation is 85°C and the time is 30 minutes; the temperature of the water bath sterilization is 85°C and the time is 30 minutes.

[0045] In the above technical scheme, the preparation method of the yellow catfish extract is: take yellow catfish, remove the internal organs, crush it to obtain fish paste, add water 10 times the mass of the fish paste to the fish paste, add Propionibacterium schreiberis and Clostridium butyricum, ferment for 12 hours, sterilize in a water bath, reduce to 36°C, adjust the pH to 7.8, add trypsin, enzymolysis for 1.5 hours, then inactivate the enzyme in a water bath, reduce to room temperature, filter with a 2kDa ultrafiltration membrane, obtain liquid A below 2kDa, freeze-dry, and obtain the yellow catfish extract.

[0046] The amount of Propionibacterium schizophylla added is 1% of the mass of the fish paste; the amount of Clostridium butyricum added is 0.5% of the mass of the fish paste; the amount of trypsin added is 0.5% of the mass of the fish paste; the temperature of the water bath sterilization is 85°C and the time is 30 minutes; the temperature of the water bath enzyme inactivation is 85°C and the time is 30 minutes.

[0047] In the above technical scheme, the preparation method of the gooseberry extract is: crush the gooseberries, add water 5 times the mass of the gooseberries, adjust the pH to 1.5, add pepsin 0.2% by mass of the gooseberries, hydrolyze at 36°C for 1 hour, inactivate the enzyme in a water bath at 85°C for 30 minutes, cool to room temperature, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, spray dry, and obtain the gooseberry extract.

[0048] In the above technical scheme, the preparation method of the blueberry extract is: crushing the blueberries, adding water 5 times the mass of the blueberries, adjusting the pH to 1.5, adding 0.2% of the mass of pepsin of the blueberries, enzymolysis at 36°C for 1 hour, inactivating the enzyme in a water bath at 85°C for 30 minutes, cooling to 36°C, adjusting the pH to 7.8, adding 0.2% of the mass of trypsin of the blueberries, enzymolysis for 1.5 hours, inactivating the enzyme in a water bath at 85°C for 30 minutes, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane, obtaining a liquid A below 2kDa, spray drying, and obtaining a blueberry extract.

[0049] The preparation method of the above-mentioned whitening, anti-aging and wrinkle-reducing composition comprises the following steps:

[0050] According to the mass fraction, the purified water is heated to 75°C, carbomer and niacinamide are added, mixed evenly, cooled to 50°C, resveratrol and lecithin are added, mixed evenly, cooled to 35°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, cooled to obtain a composition.

[0051] Example 3

[0052] A composition for whitening, anti-aging and wrinkle reduction is prepared from the following raw materials in parts by mass: 3 parts of bamboo fungus extract, 1.5 parts of charcoalwort extract, 2.5 parts of yellow catfish extract, 0.6 parts of gooseberry extract, 0.6 parts of blueberry extract, 0.3 parts of resveratrol, 0.5 parts of niacinamide, 2 parts of lecithin, 2 parts of carbomer and 60 parts of purified water.

[0053] In the above technical scheme, the preparation method of the bamboo fungus extract is: taking fresh bamboo fungus to break the spores and crushing them, adding 0.20 mol / L saline, soaking them for 2 hours using 40KHz ultrasound, centrifuging, taking the supernatant, adding 4.5 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water with a mass 10 times that of the protein precipitate, adjusting the pH to 7.5, adding papain, enzymolysis at 45°C for 1.5 hours, inactivating the enzyme in a water bath, cooling to 40°C, adding subtilisin, enzymolysis for 2 hours, inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray drying, and obtaining a bamboo fungus extract.

[0054] The amount of salt water added is 10 times the mass of bamboo fungus; the amount of papain added is 1% of the mass of protein precipitation; the amount of subtilisin added is 0.6% of the mass of protein precipitation; the temperature of water bath enzyme inactivation is 95°C and the time is 40 minutes.

[0055] In the above technical scheme, the preparation method of the charcoal motherwort extract is: crush the charcoal motherwort, add water 10 times the mass of the charcoal motherwort, adjust the pH to 5.5, add hemicellulase, enzymolysis at 60°C for 2 hours, inactivate the enzyme in a water bath, reduce the temperature to 40°C, adjust the pH to 7.4, add Bacillus subtilis, ferment at 40°C for 72 hours, sterilize in a water bath, reduce the temperature to 38°C, adjust the pH to 8.5, add trypsin, enzymolysis for 2 hours, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray dry, and obtain the charcoal motherwort extract.

[0056] The amount of hemicellulase added is 3% of the mass of pyrocalyx; the amount of Bacillus subtilis added is 3% of the mass of pyrocalyx; the amount of trypsin added is 1% of the mass of pyrocalyx; the temperature of the water bath enzyme inactivation is 95°C and the time is 40 minutes; the temperature of the water bath sterilization is 100°C and the time is 40 minutes.

[0057] In the above technical scheme, the preparation method of the yellow catfish extract is: take yellow catfish, remove the internal organs, crush it to obtain fish paste, add water 12 times the mass of the fish paste to the fish paste, add Propionibacterium schreiberis and Clostridium butyricum, ferment for 15 hours, sterilize in a water bath, reduce to 38°C, adjust the pH to 8.5, add trypsin, enzymolysis for 2 hours, then inactivate the enzyme in a water bath, reduce to room temperature, filter with a 2kDa ultrafiltration membrane, obtain liquid A below 2kDa, freeze-dry, and obtain the yellow catfish extract.

[0058] The amount of Propionibacterium schizophylla added is 3% of the mass of the fish paste; the amount of Clostridium butyricum added is 2% of the mass of the fish paste; the amount of trypsin added is 1% of the mass of the fish paste; the temperature of the water bath sterilization is 100°C and the time is 40 minutes; the temperature of the water bath enzyme inactivation is 95°C and the time is 40 minutes.

[0059] In the above technical scheme, the preparation method of the gooseberry extract is: crush the gooseberries, add water 6 times the mass of the gooseberries, adjust the pH to 2.0, add pepsin 0.5% by mass of the gooseberries, hydrolyze at 38°C for 2h, inactivate the enzyme in a 95°C water bath for 40min, cool to room temperature, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, spray dry, and obtain the gooseberry extract.

[0060] In the above technical scheme, the preparation method of the blueberry extract is: crushing the blueberries, adding water 6 times the mass of the blueberries, adjusting the pH to 2.0, adding pepsin 0.5% by mass of the blueberries, enzymolysis at 38°C for 2 hours, inactivating the enzyme in a water bath at 95°C for 40 minutes, cooling to 38°C, adjusting the pH to 8.5, adding trypsin 0.5% by mass of the blueberries, enzymolysis for 2 hours, inactivating the enzyme in a water bath at 95°C for 40 minutes, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid A below 2kDa, spray drying, and obtaining a blueberry extract.

[0061] The preparation method of the above-mentioned whitening, anti-aging and wrinkle-reducing composition comprises the following steps:

[0062] According to the mass fraction, the purified water is heated to 80°C, carbomer and niacinamide are added, mixed evenly, cooled to 60°C, resveratrol and lecithin are added, mixed evenly, cooled to 40°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, and cooled to obtain a composition.

[0063] Example 4

[0064] A composition for whitening, anti-aging and wrinkle reduction is prepared from the following raw materials in parts by mass: 1 part of bamboo fungus extract, 1.2 parts of pygmy caltrop extract, 1.5 parts of yellow catfish extract, 0.3 parts of gooseberry extract, 0.3 parts of blueberry extract, 0.3 parts of resveratrol, 0.5 parts of niacinamide, 1.5 parts of lecithin, 1.5 parts of carbomer and 50 parts of purified water.

[0065] In the above technical scheme, the preparation method of the bamboo fungus extract is: taking fresh bamboo fungus to break the spores and crushing them, adding 0.15 mol / L saline, soaking them for 1 hour using 40KHz ultrasound, centrifuging, taking the supernatant, adding 4.5 mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in water with a mass 8 times that of the protein precipitate, adjusting the pH to 7.5, adding papain, enzymolysis at 40°C for 1.5 hours, inactivating the enzyme in a water bath, cooling to 30°C, adding subtilisin, enzymolysis for 2 hours, inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray drying, and obtaining a bamboo fungus extract.

[0066] The amount of salt water added is 10 times the mass of bamboo fungus; the amount of papain added is 0.3% of the mass of protein precipitation; the amount of subtilisin added is 0.6% of the mass of protein precipitation; the temperature of water bath enzyme inactivation is 85°C and the time is 40 minutes.

[0067] In the above technical scheme, the preparation method of the charcoal motherwort extract is: grind the charcoal motherwort, add water 8 times the mass of the charcoal motherwort, adjust the pH to 5.5, add hemicellulase, enzymolysis at 50°C for 2 hours, inactivate the enzyme in a water bath, reduce the temperature to 35°C, adjust the pH to 7.1, add Bacillus subtilis, ferment at 37°C for 48 hours, sterilize in a water bath, reduce the temperature to 37°C, adjust the pH to 8.2, add trypsin, enzymolysis for 1.5 hours, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, spray dry, and obtain the charcoal motherwort extract.

[0068] The amount of hemicellulase added is 1% of the mass of pyrocalyx; the amount of Bacillus subtilis added is 0.5% of the mass of pyrocalyx; the amount of trypsin added is 0.5% of the mass of pyrocalyx; the temperature of the water bath enzyme inactivation is 85°C and the time is 40 minutes; the temperature of the water bath sterilization is 85°C and the time is 40 minutes.

[0069] In the above technical scheme, the preparation method of the yellow catfish extract is: take yellow catfish, remove the internal organs, crush it to obtain fish paste, add water 10 times the mass of the fish paste to the fish paste, add Propionibacterium schreiberis and Clostridium butyricum, ferment for 13 hours, sterilize in a water bath, reduce to 37°C, adjust the pH to 8.2, add trypsin, enzymolysis for 1.5 hours, then inactivate the enzyme in a water bath, reduce to room temperature, filter with a 2kDa ultrafiltration membrane, obtain liquid A below 2kDa, freeze-dry, and obtain the yellow catfish extract.

[0070] The amount of Propionibacterium schizophylla added is 1.5% of the mass of the fish paste; the amount of Clostridium butyricum added is 0.8% of the mass of the fish paste; the amount of trypsin added is 0.8% of the mass of the fish paste; the temperature of the water bath sterilization is 95°C and the time is 30 minutes; the temperature of the water bath enzyme inactivation is 95°C and the time is 30 minutes.

[0071] In the above technical scheme, the preparation method of the gooseberry extract is: crush the gooseberries, add water 5 times the mass of the gooseberries, adjust the pH to 2.0, add pepsin 0.2% by mass of the gooseberries, hydrolyze at 37°C for 1 hour, inactivate the enzyme in a 95°C water bath for 30 minutes, cool to room temperature, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, spray dry, and obtain the gooseberry extract.

[0072] In the above technical scheme, the preparation method of the blueberry extract is: crushing the blueberries, adding water 5 times the mass of the blueberries, adjusting the pH to 2.0, adding 0.2% of the mass of pepsin of the blueberries, enzymolysis at 37°C for 1 hour, inactivating the enzyme in a water bath at 95°C for 30 minutes, and after cooling to 37°C, adjusting the pH to 8.2, adding 0.5% of the mass of trypsin of the blueberries, enzymolysis for 1.5 hours, inactivating the enzyme in a water bath at 95°C for 30 minutes, cooling to room temperature, filtering with a 2kDa ultrafiltration membrane, obtaining a liquid A below 2kDa, spray drying, and obtaining a blueberry extract.

[0073] The preparation method of the above-mentioned whitening, anti-aging and wrinkle-reducing composition comprises the following steps:

[0074] According to the mass fraction, the purified water is heated to 75°C, carbomer and niacinamide are added, mixed evenly, cooled to 50°C, resveratrol and lecithin are added, mixed evenly, cooled to 37°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, cooled to obtain a composition.

[0075] Preparation Comparative Example 1

[0076] The composition does not contain bamboo fungus extract; other parameters and methods are the same as in Example 1.

[0077] Preparation Comparative Example 2

[0078] The composition does not contain the extract of Herba Eupatorii; other parameters and methods are the same as those in Example 1.

[0079] Preparation Comparative Example 3

[0080] The composition does not contain the Pelteobagrus vachelli extract; other parameters and methods are the same as those in Example 1.

[0081] Preparation Comparative Example 4

[0082] The composition does not contain gooseberry extract and blueberry extract; other parameters and methods are the same as those in Example 1.

[0083] Preparation Comparative Example 5

[0084] The composition does not contain the extract of Dictyophora indica, the extract of Herba Lycopodii and the extract of Psoralea corylifolia. Other parameters and methods are the same as those in Example 1.

[0085] The compositions of the above-mentioned embodiments and the combinations of the comparative examples were subjected to the following tests.

[0086] 1. Patch test

[0087] 20 female subjects were selected, and 0.025 g of the composition sample of each example was placed in a spot tester chamber and applied to the inner arm of the subject. The spot tester was removed after 24 hours, and the skin condition was observed within 48 hours. No adverse reactions were observed, and the ingredients were all safe and non-irritating.

[0088] 2. Tyrosinase inhibition test

[0089] 200 μL of the composition was added to a well plate inoculated with B16F10 cells, and pure water was used instead of the composition as a blank sample. The absorbance was measured after 48 hours of culture, and the cell survival rate was calculated. The activity of tyrosinase in the B16F10 cells was measured by an ELISA assay method, and the inhibition rate was calculated. The content of melanin in the cells was measured, and the melanin inhibition rate was calculated. The test results are shown in Table 1 below:

[0090] Table 1 Tyrosinase inhibition test results

[0091]

[0092] From the above results, it can be seen that the compositions of Examples 1 to 4 have good anti-melanin deposition effects and have good skin whitening and lightening effects. From the comparative examples, it can be seen that the bamboo fungus extract, gooseberry extract and blueberry extract have good effects of inhibiting tyrosinase and melanin deposition, and promote obvious whitening effects.

[0093] 3. Hydroxyl free radical scavenging test

[0094] 10 μL of 1.25 mg / mL salicylic acid solution and 2.5 mg / mL ferrous sulfate solution were added to the ELISA plate, followed by 180 μL of pure water, followed by 40 μL of the composition sample, and finally 10 μL of 0.03% mass concentration of H2O2. After reacting for 10 minutes, the absorbance was measured using an ELISA instrument, and compared with the blank control sample (40 μL of the moisturizing anti-aging composition was replaced with 40 μL of pure water), and the hydroxyl radical scavenging rate of the sample was calculated; the test results are shown in Table 2 below:

[0095] Table 2 Hydroxyl radical scavenging test results

[0096]

[0097]

[0098] From the above results, it can be seen that the compositions of Examples 1 to 4 have a good ability to scavenge hydroxyl free radicals; from Comparative Examples 1, 2 and 4, it can be seen that the bamboo fungus extract, the charcoal motherwort extract, the gooseberry extract and the blueberry extract have a good promoting effect of scavenging hydroxyl free radicals, and can greatly improve the antioxidant and anti-aging abilities of cells; from Comparative Example 5, it can be seen that the bamboo fungus extract, the charcoal motherwort extract and the yellow catfish extract have a synergistic effect, and when used in combination, the synergistic scavenging effect of hydroxyl free radicals is very prominent.

[0099] 4. Skin elasticity and whitening effect test

[0100] 72 subjects (23-38 years old), half of whom were male and half were female; they were equally divided into 9 groups, with 8 people in each group (4 males and 4 females); VISA facial image analysis system and Image-Pro Plus image analysis software were used to detect facial ITA° value, and the larger the ITA° value, the brighter the skin color; skin elasticity tester MPA580 and ReviewmeterRV600 probe were used to detect skin elasticity and roughness parameters; initial parameters were detected before using the composition; then each group of subjects used each composition sample respectively, and evenly applied the composition after cleansing the face in the morning and evening every day for 2 months, and the parameters after 2 months were measured; the change rate of ITA° value, skin elasticity change rate, and roughness change rate were calculated; the test results are shown in Table 3 below.

[0101] ITA° value change rate (%) = (ITA° value after 2 months - ITA° value before use) / ITA° value before use × 100%

[0102] Skin elasticity change rate (%) = (skin elasticity after 2 months - skin elasticity before use) / skin elasticity before use × 100%

[0103] Roughness change rate (%) = (roughness before use - roughness after 2 months) / roughness before use × 100%

[0104] Table 3 Skin elasticity and whitening effect test results

[0105]

[0106]

[0107] As can be seen from the above results, the whitening, skin elasticity restoration, skin delicate restoration, and wrinkle fading effects of Examples 1 to 4 are good, and the whitening and anti-aging effects can be greatly improved after 2 months of use, and skin aging can be delayed. From Comparative Examples 1 and 4, it can be seen that the bamboo fungus extract, gooseberry extract, and blueberry extract have good permeability and absorption, and can quickly and significantly improve the whitening effect and brighten the skin. From Comparative Example 3, it can be seen that the yellow catfish extract has an excellent effect of improving skin elasticity and roughness. The yellow catfish extract contains rich small molecule peptides, which can promote cells to produce collagen, cooperate with other ingredients to restore cell vitality, and improve skin conditions. From Comparative Example 5, it can be seen that the bamboo fungus extract, the charcoal mother grass extract, and the yellow catfish extract are used in combination, and have a good synergistic effect, which can greatly improve the effects of whitening, restoring skin elasticity, restoring skin delicate restoration, and wrinkle fading.

Claims

1. A composition for whitening, anti-aging and wrinkle reduction, characterized in that: The composition is prepared from the following raw materials in parts by mass: 0.5 to 3 parts of bamboo fungus extract, 0.5 to 1.5 parts of charcoalwort extract, 1 to 2.5 parts of yellow catfish extract, 0.3 to 0.6 parts of gooseberry extract, 0.3 to 0.6 parts of blueberry extract, 0.05 to 0.3 parts of resveratrol, 0.05 to 0.5 parts of niacinamide, 0.5 to 2 parts of lecithin, 0.5 to 2 parts of carbomer and 50 to 60 parts of purified water; The preparation method of the bamboo fungus extract comprises the following steps: taking fresh bamboo fungus to break the spore wall and crush it, adding 0.15mol / L-0.20mol / L saline solution of 8 to 10 times the mass of the bamboo fungus, soaking it for 1 to 2 hours by ultrasonic treatment at 30KHz to 40KHz, centrifuging, taking the supernatant, adding 4mol / L-4.5mol / L ammonium sulfate solution to the supernatant until the protein is completely precipitated; centrifuging to obtain a protein precipitate; resuspending the protein precipitate in 8 to 10 times the mass of the protein In the water of the precipitate, adjust the pH to 7.0-7.5, add 0.3%-1% papain by weight of the protein precipitate, perform enzymolysis at 40-45°C for 1h-1.5h, inactivate the enzyme in a water bath, and after cooling to 30-40°C, add 0.2%-0.6% subtilisin by weight of the protein precipitate, perform enzymolysis for 1.5h-2h, inactivate the enzyme in a water bath, and after cooling to room temperature, filter with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray dry to obtain a bamboo fungus extract; The preparation method of the charcoal motherwort extract is as follows: grind the charcoal motherwort, add water 8 to 10 times the mass of the charcoal motherwort, adjust the pH to 4.0 to 5.5, add hemicellulase 1% to 3% by mass of the charcoal motherwort, perform enzymolysis at 50 to 60° C. for 1.5 to 2 hours, inactivate the enzyme in a water bath, reduce the temperature to 35 to 40° C., adjust the pH to 6.8 to 7.4, add Bacillus subtilis 0.5% to 3% by mass of the charcoal motherwort, ferment at 35 to 40° C. for 48 to 72 hours, sterilize in a water bath, reduce the temperature to 36 to 38° C., adjust the pH to 7.8 to 8.5, add trypsin 0.5% to 1% by mass of the charcoal motherwort, perform enzymolysis for 1.5 to 2 hours, inactivate the enzyme in a water bath, reduce the temperature to room temperature, filter with a 2 kDa ultrafiltration membrane to obtain a liquid below 2 kDa, and spray dry to obtain the charcoal motherwort extract; The preparation method of the yellow catfish extract is as follows: taking yellow catfish, removing the internal organs, and crushing to obtain fish paste, adding water 10 to 12 times the mass of the fish paste to the fish paste, adding 1% to 3% of the mass of Propionibacterium schreiberis and 0.5% to 2% of the mass of Clostridium butyricum, fermenting for 12 hours to 15 hours, sterilizing in a water bath, cooling to 36° C. to 38° C., adjusting the pH to 7.8 to pH 8.5, adding 0.5% to 1% of the mass of trypsin, enzymolysis for 1.5 hours to 2 hours, then inactivating the enzyme in a water bath, cooling to room temperature, filtering with a 2 kDa ultrafiltration membrane to obtain a liquid below 2 kDa, and freeze-drying to obtain the yellow catfish extract; The preparation method of the gooseberry extract comprises: crushing the gooseberries, adding water 5 to 6 times the mass of the gooseberries, adjusting the pH to 1.5 to pH 2.0, adding pepsin 0.2% to 0.5% by mass of the gooseberries, performing enzymatic hydrolysis at 36° C. to 38° C. for 1 h to 2 h, inactivating the enzyme in a water bath at 85° C. to 95° C. for 30 min to 40 min, cooling to room temperature, filtering with a 2 kDa ultrafiltration membrane, taking a filtrate less than 2 kDa, and spray drying to obtain the gooseberry extract; The preparation method of the blueberry extract comprises the following steps: crushing blueberries, adding water 5 to 6 times the mass of the blueberries, adjusting the pH to 1.5 to 2.0, adding pepsin in an amount of 0.2% to 0.5% by mass of the blueberries, performing enzymatic hydrolysis at 36°C to 38°C for 1 to 2 hours, inactivating the enzyme in a water bath at 85°C to 95°C for 30 to 40 minutes, cooling the temperature to 36°C to 38°C, adjusting the pH to 7.8 to 8.5, adding trypsin in an amount of 0.2% to 0.5% by mass of the blueberries, performing enzymatic hydrolysis for 1.5 to 2 hours, inactivating the enzyme in a water bath at 85°C to 95°C for 30 to 40 minutes, cooling the temperature to room temperature, filtering with a 2 kDa ultrafiltration membrane to obtain a liquid below 2 kDa, and spray drying to obtain the blueberry extract.

2. A whitening, anti-aging and wrinkle-reducing composition according to claim 1, characterized in that: In the preparation method of the bamboo fungus extract, the temperature of the water bath enzyme inactivation is 85° C. to 95° C., and the time is 30 min to 40 min.

3. The whitening, anti-aging and wrinkle-reducing composition according to claim 1, characterized in that: In the preparation method of the charcoalwort extract, the temperature of the water bath enzyme inactivation is 85°C to 95°C, and the time is 30min to 40min; the temperature of the water bath sterilization is 85°C to 100°C, and the time is 30min to 40min.

4. The whitening, anti-aging and wrinkle-reducing composition according to claim 1, characterized in that: In the preparation of the Pelteobagrus vachelli extract, the temperature of the water bath enzyme inactivation is 85°C to 95°C, and the time is 30min to 40min; the temperature of the water bath sterilization is 85°C to 100°C, and the time is 30min to 40min.

5. The method for preparing the whitening, anti-aging and wrinkle-lightening composition according to claim 1, characterized in that: The preparation method comprises the following steps: According to the mass fraction, the purified water is heated to 75°C-80°C, carbomer and niacinamide are added, mixed evenly, cooled to 50°C-60°C, resveratrol and lecithin are added, mixed evenly, cooled to 35°C-40°C, bamboo fungus extract, charcoal motherwort extract, yellow catfish extract, gooseberry extract and blueberry extract are added, mixed evenly, cooled to obtain a composition.

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