Anti-aging composition containing plant polypeptide and preparation method thereof

Abstract

The present invention relates to an anti-aging composition containing plant polypeptides and a preparation method thereof, and belongs to the technical field of cosmetics. An anti-aging composition containing plant polypeptides comprises the following components: ginkgo polypeptide, jaboticaba peel extract and hydroxypinacolone retinoic acid ester; the preparation of ginkgo polypeptide: ginkgo powder is added to deionized water for heat treatment, mixed enzyme is added for enzymolysis after cooling, inactivation is performed, anhydrous ethanol is added for uniform mixing, aging, centrifugation, and drying to obtain the composition. The present invention effectively copes with the problem of inflammatory aging by combining ginkgo extract, jaboticaba peel extract and hydroxypinacolone retinoic acid ester. It is found that hydroxypinacolone retinoic acid ester not only significantly enhances the antioxidant capacity of jaboticaba peel extract, but also plays a positive role in reducing skin inflammation. In addition, when used in combination with ginkgo polypeptide, the composition of the present invention can significantly slow down the oxidative stress apoptosis rate of cells, thereby achieving excellent anti-skin aging effect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to an anti-aging composition containing plant polypeptides and a preparation method thereof. Background Art

[0002] Skin aging, as an early indicator of systemic aging, has always been valued by researchers in the field of aging. In addition to natural aging, how to slow down skin aging is also a concern of dermatologists and skin care product developers.

[0003] Hydroxypinacolone retinoate is widely used in skin care products because it can help skin cells repair and regenerate better and inhibit melanin synthesis, improving skin tone. However, if used improperly, it may cause skin irritation or allergic reactions.

[0004] Jabuticaba, also known as pseudo-love tree, treasure fruit, and tree grape, is an evergreen plant belonging to the genus Persiana of the Myrtaceae family. The flesh, peel, and even leaves of Jabuticaba are rich in anthocyanins, polyphenols, flavonoids, tannic acid, multiple vitamins, minerals, and other bioactive substances, and have high antioxidant, anti-inflammatory, hypoglycemic and other health benefits. Jabuticaba peel is the main waste generated during the deep processing of Jabuticaba. Jabuticaba peel contains rich nutrients and bioactive substances. In actual production, Jabuticaba peel is discarded, missing out on effective resource utilization. Using Jabuticaba peel as the main raw material to develop products with antioxidant functions is rarely reported in the field of anti-aging cosmetics. Summary of the invention

[0005] The object of the present invention is to provide an anti-aging composition containing plant polypeptides and a preparation method thereof. The present invention combines ginkgo extract with jaboticaba peel extract and introduces hydroxypinacolone retinoic acid ester, thereby effectively coping with inflammatory aging problems. In addition, hydroxypinacolone retinoic acid ester not only significantly enhances the antioxidant capacity of jaboticaba peel extract, but also plays a positive role in reducing skin inflammation. In addition, when used in combination with ginkgo polypeptide, the composition of the present invention can significantly slow down the oxidative stress apoptosis rate of cells, thereby achieving excellent anti-skin aging effects.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0008]

[0009] As a preferred technical solution of the present invention, ginkgo polypeptide can remove free radicals in the body, inhibit oxidative stress reactions, reduce the degree of lipid peroxidation, and thus reduce oxidative damage to cell membranes.

[0010] As a preferred technical solution of the present invention, the jaboticaba peel extract has a strong antioxidant effect in vivo and in vitro, which is specifically manifested in the following aspects: scavenging free radicals: it can scavenges free radicals such as DPPH, ABTS+, OH·, and block free radical chain reactions; resisting lipid peroxidation: by reducing the MDA content, lipid oxidation is delayed; and the activity of antioxidant enzymes is increased: superoxide dismutase, glutathione oxidase, and catalase activity.

[0011] As a preferred technical solution of the present invention, hydroxypinacolone retinoic acid ester can induce collagen synthesis and reduce matrix collagen decomposition by inhibiting metalloproteinases, thereby thickening the skin epidermis and making the stratum corneum denser, inhibiting melanin synthesis, improving skin color, and achieving an anti-aging effect.

[0012] The preparation of the ginkgo polypeptide comprises the steps of adding ginkgo powder into deionized water for heat treatment, adding mixed enzymes for enzymolysis after cooling, inactivating with ultraviolet rays, adding anhydrous ethanol for uniform mixing, aging, centrifuging, taking the supernatant and drying the resulting product.

[0013] As a preferred technical solution of the present invention, the heat treatment is ultrasonic treatment at a frequency of 40-50kHz for 3 minutes, heating to 40-50°C and keeping warm for 10-15 minutes; the cooling is cooling to 35°C; the enzymatic hydrolysis is at 35-45°C for 1.2-1.5 hours; and the aging time is 8-10 hours.

[0014] As a preferred technical solution of the present invention, the mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7-8:0.18-0.25:80.

[0015] As a preferred technical solution of the present invention, the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 3-5:2:0.5-0.8.

[0016] As a preferred technical solution of the present invention, the present invention adopts a reasonable heat treatment process to ensure that the protein in the ginkgo nuts is not excessively damaged, so as to obtain polypeptides with excellent antioxidant capacity. Specifically, by combining ultrasound and heat preservation operations, the tissue structure of the ginkgo nuts can be effectively destroyed, the exposure degree of the protein and the number of enzymatic hydrolysis sites can be increased, and the subsequent enzymatic hydrolysis process is convenient. In addition, the introduction of cellulase can further decompose the cellulose in the plant cell wall, making it easier for subtilisin and chymotrypsin to contact and decompose the protein inside the ginkgo nuts, while promoting the precipitation of protein. In this process, subtilisin is responsible for catalyzing the hydrolysis of protein into small molecular weight polypeptides, while chymotrypsin can randomly cut peptide bonds, further promoting the decomposition of protein and generating smaller molecular weight polypeptides.

[0017] Furthermore, a plant polypeptide-containing anti-aging composition further comprises the following components by weight:

[0018]

[0019] As a preferred technical solution of the present invention, the penetration enhancer is prepared by mixing propylene glycol and niacinamide in a mass ratio of 8:1.

[0020] As a preferred technical solution of the present invention, the thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.2-1.8.

[0021] As a preferred technical solution of the present invention, the moisturizing agent is one or more of betaine, glycerin, erythritol, xylitol, glyceryl polyether-5 lactate, and sodium polyglutamate.

[0022] As a preferred technical solution of the present invention, the conditioning agent is one or more of panthenol, beeswax and salicylic acid.

[0023] The present invention also discloses a method for preparing an anti-aging composition containing plant polypeptides, the method comprising the following steps:

[0024] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0025] 2) Cool the preformed liquid to 40-45° C., add dipotassium glycyrrhizinate and a thickener, stir at 1000-1200 r / min for 50-60 min, and cool to room temperature to obtain the product.

[0026] As a preferred technical solution of the present invention, the preparation of the jaboticaba peel extract includes: drying, crushing, leaching, filtering and rotary evaporation of the jaboticaba peel, the leaching solvent is 60-70% volume concentration of ethanol aqueous solution, the leaching time is 50-60min, the leaching temperature is 65-70°C, and the solid-liquid ratio is 1g:45-50mL.

[0027] As a preferred technical solution of the present invention, the Jabuticaba peel extract is rich in a variety of biologically active substances, such as polyphenols, aromatic acids and flavonoids, and has antioxidant and anti-inflammatory effects.

[0028] Beneficial effects of the present invention:

[0029] The present invention innovatively combines ginkgo extract with jaboticaba peel extract and introduces hydroxypinacolone retinoic acid ester, thereby effectively coping with inflammatory aging problems. In addition, hydroxypinacolone retinoic acid ester not only significantly enhances the antioxidant capacity of jaboticaba peel extract, but also plays a positive role in reducing skin inflammation. In addition, when used in combination with ginkgo polypeptide, the composition of the present invention can significantly slow down the oxidative stress apoptosis rate of cells, thereby achieving excellent anti-skin aging effects. DETAILED DESCRIPTION

[0030] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0031] Example 1

[0032] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0033]

[0034] The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:1;

[0035] The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.2;

[0036] The moisturizing agent is a mixture of betaine and glycerin in a mass ratio of 1:1;

[0037] The conditioning agent is panthenol;

[0038] The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding a mixed enzyme for enzymolysis, inactivating with ultraviolet light, adding anhydrous ethanol, mixing evenly, aging, centrifuging, taking the supernatant and drying, and obtaining the polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 40 kHz for 3 minutes, heating to 40° C. and keeping the temperature for 10 minutes; the cooling comprises cooling to 35° C.; the enzymolysis comprises enzymolysis at 35° C. for 1.2 hours; and the aging time is 8 hours;

[0039] The mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7:0.18:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 3:2:0.5;

[0040] A method for preparing an anti-aging composition containing plant polypeptides comprises the following steps:

[0041] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0042] 2) Cool the preformed liquid to 40° C., add dipotassium glycyrrhizinate and a thickener, stir at 1000 r / min for 50 min, and cool to room temperature to obtain the product.

[0043] Example 2

[0044] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0045]

[0046] The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:1;

[0047] The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.4;

[0048] The moisturizing agent is a mixture of betaine and glycerin in a mass ratio of 1:1;

[0049] The conditioning agent is panthenol;

[0050] The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding a mixed enzyme for enzymolysis, inactivating with ultraviolet light, adding anhydrous ethanol, mixing evenly, aging, centrifuging, taking the supernatant and drying, and obtaining the polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 42kHz for 3 minutes, heating to 42°C, and keeping the temperature for 11 minutes; the cooling comprises cooling to 35°C; the enzymolysis comprises enzymolysis at 38°C for 1.3 hours; and the aging time is 8.5 hours;

[0051] The mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7.2:0.2:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 3.5:2:0.57;

[0052] A method for preparing an anti-aging composition containing plant polypeptides comprises the following steps:

[0053] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0054] 2) Cool the preformed liquid to 41° C., add dipotassium glycyrrhizinate and a thickener, stir at 1050 r / min for 52 min, and cool to room temperature to obtain the product.

[0055] Example 3

[0056] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0057]

[0058]

[0059] The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:1;

[0060] The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.5;

[0061] The moisturizing agent is a mixture of betaine and glycerin in a mass ratio of 1:1;

[0062] The conditioning agent is panthenol;

[0063] The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding a mixed enzyme for enzymolysis, inactivating with ultraviolet light, adding anhydrous ethanol, mixing evenly, aging, centrifuging, taking the supernatant and drying, and obtaining the polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 45kHz for 3 minutes, heating to 45°C, and keeping the temperature for 12 minutes; the cooling comprises cooling to 35°C; the enzymolysis comprises enzymolysis at 40°C for 1.35 hours; and the aging time is 9 hours;

[0064] The mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7.5:0.22:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 4:2:0.65;

[0065] A method for preparing an anti-aging composition containing plant polypeptides comprises the following steps:

[0066] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0067] 2) Cool the preformed liquid to 42° C., add dipotassium glycyrrhizinate and a thickener, stir at 1100 r / min for 55 min, and cool to room temperature to obtain the product.

[0068] Example 4

[0069] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0070]

[0071] The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:1;

[0072] The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.6;

[0073] The moisturizing agent is a mixture of betaine and glycerin in a mass ratio of 1:1;

[0074] The conditioning agent is panthenol;

[0075] The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding a mixed enzyme for enzymolysis, inactivating with ultraviolet light, adding anhydrous ethanol, mixing evenly, aging, centrifuging, taking the supernatant and drying, and obtaining the polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 48 kHz for 3 minutes, heating to 48° C., and keeping the temperature for 14 minutes; the cooling comprises cooling to 35° C.; the enzymolysis comprises enzymolysis at 42° C. for 1.42 hours; and the aging time is 9.5 hours;

[0076] The mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7.8:0.23:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 4.5:2:0.7;

[0077] A method for preparing an anti-aging composition containing plant polypeptides comprises the following steps:

[0078] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0079] 2) Cool the preformed liquid to 44° C., add dipotassium glycyrrhizinate and a thickener, stir at 1150 r / min for 58 min, and cool to room temperature to obtain the product.

[0080] Example 5

[0081] A plant polypeptide-containing anti-aging composition comprises the following components by weight:

[0082]

[0083] The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:1;

[0084] The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.8;

[0085] The moisturizing agent is a mixture of betaine and glycerin in a mass ratio of 1:1;

[0086] The conditioning agent is panthenol;

[0087] The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding a mixed enzyme for enzymolysis, inactivating with ultraviolet light, adding anhydrous ethanol, mixing evenly, aging, centrifuging, taking the supernatant and drying, and obtaining the polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 50 kHz for 3 minutes, heating to 50° C., and keeping the temperature for 15 minutes; the cooling comprises cooling to 35° C.; the enzymolysis comprises enzymolysis at 45° C. for 1.5 hours; and the aging time is 10 hours;

[0088] The mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:8:0.25:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin in a mass ratio of 5:2:0.8;

[0089] A method for preparing an anti-aging composition containing plant polypeptides comprises the following steps:

[0090] 1) Heat deionized water to 70° C., add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a preformed solution;

[0091] 2) Cool the preformed liquid to 45° C., add dipotassium glycyrrhizinate and a thickener, stir at 1200 r / min for 60 min, and cool to room temperature to obtain the product.

[0092] Comparative Example 1

[0093] Compared with Example 4, the difference is that no heat treatment is performed, and the other components, preparation steps and parameters are the same.

[0094] Comparative Example 2

[0095] Compared with Example 4, the difference is that no cellulase is added, the mixed enzyme is prepared by mixing subtilisin and chymotrypsin in a mass ratio of 2:0.7, and the other components, preparation steps and parameters are the same.

[0096] Comparative Example 3

[0097] Compared with Example 4, the difference is that no subtilisin is added, the mixed enzyme is prepared by mixing cellulase and chymotrypsin in a mass ratio of 4.5:0.7, and the other components, preparation steps and parameters are the same.

[0098] Comparative Example 4

[0099] Compared with Example 4, the difference is that chymotrypsin is not added, the mixed enzyme is prepared by mixing cellulase and subtilisin in a mass ratio of 4.5:2, and the other components, preparation steps and parameters are the same.

[0100] Comparative Examples 5-8

[0101] Compared with Example 4, the difference is that the weight proportions of ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester and dipotassium glycyrrhizinate are as shown in Table 1, and the other components, preparation steps and parameters are the same.

[0102] Table 1 (Unit: parts by weight)

[0103]

[0104] Comparative Example 9

[0105] Compared with Example 4, the difference is that 4 parts of hydroxypinacolone retinoic acid ester are added, and the other components, preparation steps and parameters are the same.

[0106] Irritation evaluation test

[0107] 70 adult subjects with healthy skin were selected and divided into 14 groups, with 5 persons in each group, and the compositions prepared in Examples 1-5 and Comparative Examples 1-9 were tested accordingly, and the results were averaged.

[0108] Test method: After washing the face in the morning and evening, apply the composition prepared in each embodiment and comparative example on the left side, and the adult volunteers score the irritation of the composition after using it for 7 consecutive days, during which time no other skin care products are used;

[0109] The rating scale is 0-10, where 0 means no obvious irritation, 1-2 means slight irritation, 3-4 means strong irritation, 5-6 means moderate irritation, 7-8 means strong irritation, and 9-10 means severe irritation. The test results are shown in Table 2.

[0110] Table 2

[0111]

[0112]

[0113] From the results in Table 2, combined with the analysis of Examples 1-5 and Comparative Examples 1-9, it can be seen that the combination of Jabuticaba peel extract and dipotassium glycyrrhizinate can significantly reduce the skin irritation effects of hydroxypinacolone retinoic acid ester and niacinamide.

[0114] Cellular antioxidant efficacy evaluation experiment

[0115] First, a sample solution with a concentration of 0.5% (Example 1-5, Comparative Example 1-9) was prepared with serum-free DMEM culture medium, and sterilized by filtration with a 0.22 μm filter membrane. Then, a dichlorofluorescein diacetate (DCFH-DA) probe solution was prepared: diluting at a ratio of 0.375 uLDCFH-DA: 1 mL PBS.

[0116] HaCaT cells in the logarithmic growth phase were cultured at 1×10 5 Cells were inoculated at a density of 100 / mL in a 24-well culture plate, and 16 wells were selected. Each well contained 2 mL of cell suspension and was placed in a carbon dioxide incubator at 37°C and 5% CO2 for 24 hours. 2 , irradiation intensity for 1h, UVB with 700μW / cm 2 The cells were irradiated with an intensity of 100 μg / mL for 3 min, and the normal control group was not irradiated. After irradiation, the old culture medium was discarded, and the sample solution was added to the experimental group, and serum-free DMEM culture medium was added to the model group and the normal control group, with 2 mL per well. After continuing to culture for 16 h, all the culture medium was discarded and washed twice with PBS. 1.5 mL of DCFH-DA solution was added to each well, and the cells were placed in a 37 ° C cell culture incubator for another 30 min, and mixed every 5 min to allow the probe to fully bind. The probe was discarded, washed twice with preheated serum-free culture medium, and 1 mL of serum-free culture medium was added to each well and incubated at 37 ° C for 10 min. After washing once with PBS, the cells were digested with trypsin, washed twice with PBS, resuspended with 300 μL PBS, and detected by dual-channel flow cytometer (cells were filtered with a 100-mesh filter before being put on the machine), FL1-H channel, and 10,000 cells were collected for each sample.

[0117] ROS clearance rate = (model group - experimental group) / model group × 100%;

[0118] The test results are shown in Table 3;

[0119] Table 3

[0120]

[0121]

[0122] From the test results in Table 3, it can be seen that compared with Comparative Examples 1-9, Examples 1-5 of the present invention have a significant synergistic effect among the ginkgo polypeptide, the jaboticaba peel extract and the hydroxypinacolone retinoic acid ester, and achieve a significant antioxidant effect through their excellent free radical scavenging ability. The three can directly or indirectly regulate the synthesis process of melanin through the joint action of multiple mechanisms, thereby achieving a significant anti-aging effect.

[0123] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. An anti-aging composition containing plant polypeptides, characterized in that: The components include the following by weight: 2-4 servings of Ginkgo polypeptide 5-8 parts of Jabuticaba peel extract 1.5-2.0 parts of hydroxypinacolone retinoic acid ester Dipotassium glycyrrhizinate 0.2-0.4 parts; The preparation of the ginkgo polypeptide comprises the following steps: adding ginkgo powder to deionized water for heat treatment, cooling, adding mixed enzyme for enzymolysis, inactivating with ultraviolet rays, adding anhydrous ethanol for uniform mixing, aging, centrifuging, taking the supernatant and drying, and obtaining the ginkgo polypeptide; the heat treatment comprises ultrasonic treatment at a frequency of 40-50kHz for 3 minutes, heating to 40-50°C, and keeping the temperature for 10-15 minutes; the cooling comprises cooling to 35°C; the enzymolysis comprises enzymolysis at 35-45°C for 1.2-1.5 hours; the aging time is 8-10 hours; the mass ratio of the ginkgo powder, deionized water, mixed enzyme and anhydrous ethanol is 1.2:7-8:0.18-0.25:80; the mixed enzyme is prepared by mixing cellulase, subtilisin and chymotrypsin at a mass ratio of 3-5:2:0.5-0.

8.

2. The anti-aging composition containing plant polypeptides according to claim 1, characterized in that: The following components are also included by weight: Penetration enhancer 14-18 parts Thickener 0.3-0.5 parts 5-8 parts moisturizer Conditioner 0.6-0.8 parts 60-70 parts of deionized water.

3. The anti-aging composition containing plant polypeptides according to claim 2, characterized in that: The penetration enhancer is prepared by mixing propylene glycol and nicotinamide in a mass ratio of 8:

1.

4. The anti-aging composition containing plant polypeptides according to claim 2, characterized in that: The thickener is a mixture of carbomer and sodium carrageenan in a mass ratio of 6:1.2-1.

8.

5. The anti-aging composition containing plant polypeptides according to claim 2, characterized in that: The moisturizing agent is one or more of betaine, glycerin, erythritol, xylitol, glyceryl polyether-5 lactate and sodium polyglutamate.

6. The anti-aging composition containing plant polypeptides according to claim 2, characterized in that: The conditioning agent is one or more of panthenol, beeswax and salicylic acid.

7. A method for preparing the anti-aging composition containing plant polypeptides as claimed in any one of claims 2 to 6, characterized in that: The preparation method comprises the following steps: 1) Heat deionized water to 70°C, add ginkgo polypeptide, jaboticaba peel extract, hydroxypinacolone retinoic acid ester, penetration enhancer, moisturizer and conditioner, mix well, and stir for 35-45 minutes to obtain a pre-made solution; 2) Cool the pre-made liquid to 40-45°C, add dipotassium glycyrrhizinate and thickener, stir at 1000-1200 r / min for 50-60 min, and cool to room temperature to obtain the product.

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