A composition for enhancing immunity, a health food, and a preparation method thereof

Abstract

The application belongs to the technical field of health care products, and specifically discloses a composition for enhancing immunity, a health care food and a preparation method, which comprises the following components in parts by weight: 6-10 parts of ganoderma extract, 10-15 parts of astragalin, 12-18 parts of collagen, 18-22 parts of chitosan oligosaccharide, 19-24 parts of polygonatum extract and 24-28 parts of selenium-rich yeast. The ganoderma extract, the astragalin, the collagen, the chitosan oligosaccharide, the polygonatum extract and the selenium-rich yeast are combined, and the synergistic effect of the ganoderma extract, the astragalin, the collagen, the chitosan oligosaccharide, the polygonatum extract and the selenium-rich yeast is utilized to improve the immunity of the human body from different angles, aspects and all aspects.

Description

Technical Field

[0001] This invention relates to the field of health product technology, specifically to a composition for enhancing immunity, a health food, and a preparation method thereof. Background Technology

[0002] After hundreds of thousands of years of evolution, humans have developed the ability to resist invasion by foreign viruses and bacteria; this ability is called immunity. Human immunity mainly consists of three parts: innate immunity, which is the foundation of human immune function and is related to factors such as heredity and age, generally changing with age; specific immunity, which refers to the immunity produced after exposure to a specific antigen (such as a vaccine); and variable immunity, which mainly refers to the immune capacity that can be adjusted and improved through acquired means.

[0003] The decline in human immunity has become a widespread problem. The main consequence of a weakened immune system is that the body is more susceptible to viruses, bacteria, and other pathogens. The most direct manifestation of this is that one is particularly prone to catching colds, getting sick and taking medication becomes commonplace, and the recovery time from each illness is often very long.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] This invention provides an immune-enhancing composition, a health food product, and a preparation method thereof, wherein the composition can significantly enhance immunity.

[0006] The present invention solves its technical problem by adopting the following technical solution: An immune-enhancing composition comprising the following components in parts by weight: 6-10 parts Ganoderma lucidum extract, 10-15 parts astragaloside, 12-18 parts collagen, 18-22 parts chitosan oligosaccharide, 19-24 parts Polygonatum odoratum extract, and 24-28 parts selenium-enriched yeast.

[0007] Among them, Ganoderma lucidum extract has a balancing and regulating effect on immunity. It can both inhibit excessive immune response and enhance the state of low immunity. It can enhance the vitality of immune cells, improve the body's immunity, block the release of allergy reaction mediators, prevent the occurrence of allergic reactions, and play a good role in adjuvant treatment.

[0008] Among them, astragaloside can help fight free radical damage, slow down cell aging, and enhance the function of the body's immune system, improving the body's resistance. It can reduce inflammation and relieve joint pain, which also helps maintain the health of the immune system. It can also regulate the immune system and reduce the occurrence of allergic reactions, further demonstrating its positive effect on immunity.

[0009] Collagen is a biological macromolecule and a major component of connective tissue, accounting for 25% to 35% of total human protein. It is one of the most abundant and widely distributed functional proteins in the human body. As an essential nutrient, collagen promotes bone growth and development, maintains normal skin function, and can bind with immune cells to enhance their function and accelerate their division and differentiation, thereby improving the body's immunity. Collagen plays a stabilizing role in the immune system, reducing problems and thus enhancing immunity. Furthermore, collagen contains a large amount of antioxidants, effectively scavenging free radicals and enhancing the immune system's antioxidant capacity, improving the body's self-repair capabilities and making it more effective at fighting disease.

[0010] Chitosan oligosaccharides can directly act on the central immune organs and hematopoietic stem cells in the human body, increasing the number and improving the quality of immune cells during differentiation, thereby fundamentally enhancing the body's immunity. Furthermore, chitosan oligosaccharides can comprehensively improve human immunity through multiple pathways, including cellular immunity, humoral immunity, and non-specific immunity.

[0011] Among its components, Polygonatum odoratum extract can effectively enhance the function of phagocytes and increase antibodies in the serum, thereby improving immunity, strengthening the body, and reducing the risk of disease. Furthermore, Polygonatum odoratum extract can moisturize and increase skin hydration, maintaining skin elasticity and radiance, while also promoting blood circulation and further enhancing immunity.

[0012] Among them, selenium-enriched yeast can enhance the sensitivity and responsiveness of the body's immune system, and selenium can enhance the body's immunity. The organic selenium provided by selenium-enriched yeast can be better absorbed and utilized by the human body, thus more effectively playing its role in immune regulation. Selenium-enriched yeast also has antioxidant properties. Selenium is a powerful free radical scavenger, which can inhibit oxidative stress, reduce the accumulation of harmful substances in cells, delay aging, and reduce the risk of cancer. This antioxidant effect also helps maintain the healthy state of the immune system.

[0013] This invention creatively combines Ganoderma lucidum extract, astragaloside, collagen, chitosan oligosaccharide, Polygonatum odoratum extract, and selenium-enriched yeast, utilizing the synergistic effect of these ingredients to comprehensively enhance the body's immunity from different angles and aspects.

[0014] As a preferred embodiment of the present invention, it comprises the following components in parts by weight: 8 parts Ganoderma lucidum extract, 12 parts astragaloside, 15 parts collagen, 20 parts chitosan oligosaccharide, 20 parts Polygonatum odoratum extract, and 25 parts selenium-enriched yeast.

[0015] As a preferred embodiment of the present invention, the preparation method of the Polygonatum odoratum extract is as follows: (1) Grind Solomon's Seal into powder to a fineness of 50-400 mesh. (2) Add malic acid, malonic acid and betaine to water and mix them evenly at 70~80℃ to obtain a eutectic solvent; (3) Add the Solomon's Seal powder to the eutectic solvent, sonicate, centrifuge, and dry to obtain Solomon's Seal extract.

[0016] As a preferred embodiment of the present invention, the mass ratio of malic acid, malonic acid, betaine and water is 1:(0.5~2):(1.5~3):(4~10).

[0017] In a preferred embodiment of the present invention, the mass ratio of the Solomon's seal powder to the eutectic solvent is 1:(4~10).

[0018] In a preferred embodiment of the present invention, the power of the ultrasonic treatment is 200~500W, and the ultrasonic extraction time is 20~50min.

[0019] As a preferred embodiment of the present invention, the method for preparing the Ganoderma lucidum extract is as follows: (1) Grind Ganoderma lucidum into powder to a fineness of 50-400 mesh. (2) Add Ganoderma lucidum powder, pectinase, flavor protease and trypsin to water and hydrolyze at 48~52℃ for 3~6h to obtain hydrolysate; (3) Pass the enzymatic hydrolysate through D101 macroporous adsorption resin at a flow rate of 0.5~2 BV / h, elute at a rate of 1~2 BV / h, wash with water for 1~2 BV, wash with 60~85wt% ethanol solution for 1~2 BV, collect the ethanol eluent, dry it, and obtain Ganoderma lucidum extract.

[0020] As a preferred embodiment of the present invention, the mass ratio of Ganoderma lucidum powder, pectinase, flavor protease, trypsin and water is 1:(0.005~0.02):(0.005~0.02):(0.005~0.02):(4~10).

[0021] The present invention also provides a method for preparing an immune-enhancing composition, comprising the following steps: Ganoderma lucidum extract, astragalus glycoside, collagen, chitosan oligosaccharide, Solomon's seal extract, and selenium-enriched yeast were mixed evenly and then atomized and dried to obtain an immune-enhancing composition.

[0022] The present invention also provides the application of an immune-enhancing composition in the preparation of health food products.

[0023] The beneficial effects of this invention are as follows: This invention combines Ganoderma lucidum extract, astragaloside, collagen, chitosan oligosaccharide, Polygonatum odoratum extract, and selenium-enriched yeast, and utilizes the synergistic effect of Ganoderma lucidum extract, astragaloside, collagen, chitosan oligosaccharide, Polygonatum odoratum extract, and selenium-enriched yeast to comprehensively improve the body's immunity from different angles and aspects. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0026] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0027] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0028] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0029] The astragaloside, collagen, chitosan oligosaccharide, and selenium-enriched yeast of this invention are all commercially available food-grade products.

[0030] The formulations of Examples 1-3 and Comparative Examples 1-10 are shown in Table 1 (all figures are parts by weight).

[0031] The preparation methods of the immune-enhancing compositions of Examples 1-3 and Comparative Examples 1-10 include the following steps: Mix the raw materials evenly according to the formula, atomize and dry them to obtain a composition that enhances immunity.

[0032] The preparation methods of Ganoderma lucidum extracts in Examples 1-3 and Comparative Examples 2-10 are as follows: (1) Ganoderma lucidum is pulverized to 200 mesh to obtain Ganoderma lucidum powder; (2) Add Ganoderma lucidum powder, pectinase, flavor protease and trypsin to water and enzymatically hydrolyze at 50°C for 5 hours to obtain enzymatic hydrolysate; the mass ratio of Ganoderma lucidum powder, pectinase, flavor protease, trypsin and water is 1:0.01:0.01:0.01:8. (3) The enzymatic hydrolysate was passed through D101 macroporous adsorption resin at a flow rate of 1 BV / h, and the elution rate was 1.5 BV / h. The solution was washed with water for 1.5 BV and then with 80 wt% ethanol solution for 1.5 BV. The ethanol solution eluent was collected, dried, and the Ganoderma lucidum extract was obtained.

[0033] The preparation methods of the Polygonatum odoratum extracts of Examples 1-3, Comparative Examples 1-4, and Comparative Examples 6-9 are as follows: (1) Polygonatum odoratum is pulverized to 200 mesh to obtain Polygonatum odoratum powder; (2) Add malic acid, malonic acid and betaine to water and mix them evenly at 70~80℃ to obtain a eutectic solvent; the mass ratio of malic acid, malonic acid and betaine and water is 1:1:2:8; (3) Add the Polygonatum odoratum powder to the eutectic solvent, sonicate at 400W for 30 min, centrifuge at 5000 rpm for 10 min, and dry to obtain the Polygonatum odoratum extract. The mass ratio of the Polygonatum odoratum powder to the solvent is 1:8.

[0034] Table 1

[0035] Comparative Example 11 The difference between Comparative Example 11 and Example 1 is that the preparation method of the Polygonatum odoratum extract is different, but everything else is the same.

[0036] The preparation method of the Polygonatum odoratum extract of Comparative Example 11 is as follows: (1) Pulverize Polygonatum odoratum to 200 mesh to obtain Polygonatum odoratum powder; (2) Add the Solomon's Seal powder to 6 times the amount of 80wt% ethanol aqueous solution and soak at 25°C for 18h. While soaking, sonicate at 200w power. After soaking, concentrate under reduced pressure until there is no ethanol smell, filter, and dry to obtain Solomon's Seal extract.

[0037] Comparative Example 12 The difference between Comparative Example 12 and Example 1 is that the preparation method of the Polygonatum odoratum extract is different, but everything else is the same.

[0038] The preparation method of the Polygonatum odoratum extract of Comparative Example 12 is as follows: (1) Pulverize Polygonatum odoratum to 200 mesh to obtain Polygonatum odoratum powder; (2) Add the Solomon's Seal powder to 6 times the amount of water and soak it twice at 45°C for 3 hours each time. While soaking, use 200W of sonication. After soaking, filter and dry to obtain Solomon's Seal extract.

[0039] Test case 1. Reagents and Materials 1.1 Experimental subjects: Kunming aged mice (10 months old), weighing 18-22g, male.

[0040] 1.2 Grouping and Dosing Animals were randomly divided into 16 groups, with 10 animals in each group. These groups were the blank control group, the examples, and the comparative examples. During the experiment, animals in each group had free access to 0.1% BSA acidified water. The blank group was given physiological saline by gavage daily, while the other groups were given the test samples by gavage once daily for 30 consecutive days. The dosage was 0.5 g / kg for all groups.

[0041] 2. Methods and Results 2.1 After administering the prescribed dose to each group of experimental mice via gavage for 30 consecutive days, the mice were euthanized by cervical dislocation, and their spleens were aseptically harvested to prepare a single-cell suspension (cell concentration of 1×10⁻⁶). 7 (units / mL).

[0042] 2.1.1 Mouse spleen cell suspensions were added to 24-well culture plates, with two replicates per treatment. 1 mL of solution was added to each well. One well contained 50 μL of ConA, and the other contained 50 μL of double-distilled water as a control. The plates were then incubated in a 5% CO2 incubator at 37°C for 48 hours. Four hours before the end of the incubation period, 50 μL of MTT (5 mg / mL) was added to each well, and incubation continued. After the incubation period, 1 mL of DMSO was added to each well, and the mixture was pipetted to dissolve the purple crystals completely. The cells were then aliquoted into 96-well culture plates, with three replicates per well. The OD value was measured at 570 nm using a microplate reader. The lymphocyte proliferation capacity was represented by subtracting the optical density value of the well with ConA from that of the well without ConA. Lymphocyte proliferation capacity = OD value of ConA wells - OD value of control wells. The results are shown in Table 2.

[0043] 2.2.2 NK cell activity assay: Mouse spleen cell suspension was added to PRMI 1640 complete culture medium to adjust the cell concentration to 4 × 10⁵ ml. -1(Target cells) 100 μl each of target cells and effector cells (effector-target ratio 50:1) were added to a U-shaped 96-well culture plate. 100 μl each of target cells and culture medium were added to the natural release wells, and 100 μl each of target cells and NP40 were added to the maximum release wells. All of the above were repeated in triplicate. The plates were incubated at 37℃ in a 5% CO2 incubator for 4 h. 100 μl of supernatant was aspirated from each well and placed in a flat-bottomed 96-well culture plate. 100 μl of lactate dehydrogenase (LDH) substrate solution was added simultaneously. The reaction was allowed to proceed for 3 min. 30 μl of 1 mol·L⁻¹ HCl was added to each well to terminate the reaction. The optical density (OD) was measured at 490 nm using a microplate reader. The results are shown in Table 2.

[0044] Table 2

[0045] As can be seen from Table 2, the composition described in this invention can effectively enhance immunity.

[0046] Comparing Examples 1 to 3, it can be seen that Example 1 is the best implementation of the present invention and has the best effect on enhancing immunity.

[0047] Comparing Example 1 with Comparative Examples 1-10, it can be seen that the Ganoderma lucidum extract, astragaloside, collagen, chitosan oligosaccharide, Polygonatum odoratum extract, and selenium-enriched yeast described in this invention have a significant synergistic effect in improving immunity. The absence of any one of them, or the use of other raw materials as substitutes, will lead to a significant decrease in the effect of improving immunity.

[0048] Comparing Example 1 with Comparative Examples 11-12, it can be seen that the Polygonatum odoratum extract of the present invention can significantly improve immunity. Moreover, the Polygonatum odoratum extract prepared by different methods has different effects on improving immunity. The Polygonatum odoratum extract prepared by the method of the present invention can more significantly improve immunity.

[0049] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for enhancing immunity, characterized in that, It includes the following components by weight: 6-10 parts Ganoderma lucidum extract, 10-15 parts astragaloside, 12-18 parts collagen, 18-22 parts chitosan oligosaccharide, 19-24 parts Polygonatum odoratum extract, and 24-28 parts selenium-enriched yeast. The preparation method of the Polygonatum odoratum extract is as follows: (1) Grind Solomon's Seal into powder to a fineness of 50-400 mesh. (2) Add malic acid, malonic acid and betaine to water and mix them evenly at 70~80℃ to obtain a eutectic solvent; (3) Add the Polygonatum odoratum powder to the eutectic solvent, sonicate, centrifuge, and dry to obtain Polygonatum odoratum extract; The preparation method of the Ganoderma lucidum extract is as follows: (1) Grind Ganoderma lucidum into powder to a fineness of 50-400 mesh. (2) Add Ganoderma lucidum powder, pectinase, flavor protease and trypsin to water and enzymatically hydrolyze at 48~52℃ for 3~6h to obtain enzymatic hydrolysate; (3) Pass the enzymatic hydrolysate through D101 macroporous adsorption resin at a flow rate of 0.5~2 BV / h, elute at a rate of 1~2 BV / h, wash with water for 1~2 BV, wash with 60~85wt% ethanol solution for 1~2 BV, collect the ethanol eluent, dry it, and obtain Ganoderma lucidum extract.

2. The immune-enhancing composition according to claim 1, characterized in that, It includes the following components by weight: 8 parts Ganoderma lucidum extract, 12 parts astragaloside, 15 parts collagen, 20 parts chitosan oligosaccharide, 20 parts Polygonatum odoratum extract, and 25 parts selenium-enriched yeast.

3. The immune-enhancing composition according to claim 1, characterized in that, The mass ratio of malic acid, malonic acid, betaine, and water is 1:(0.5~2):(1.5~3):(4~10).

4. The immune-enhancing composition according to claim 1, characterized in that, The mass ratio of the Solomon's Seal powder to the eutectic solvent is 1:(4~10).

5. The immune-enhancing composition according to claim 1, characterized in that, The ultrasonic treatment power is 200~500W, and the ultrasonic extraction time is 20~50min.

6. The immune-enhancing composition according to claim 1, characterized in that, The mass ratio of Ganoderma lucidum powder, pectinase, flavor protease, trypsin, and water is 1:(0.005~0.02):(0.005~0.02):(0.005~0.02):(4~10).

7. A method for preparing the immune-enhancing composition according to any one of claims 1 to 6, characterized in that, Includes the following steps: Ganoderma lucidum extract, astragalus glycoside, collagen, chitosan oligosaccharide, Solomon's seal extract, and selenium-enriched yeast were mixed evenly and then atomized and dried to obtain an immune-enhancing composition.

8. The use of the immune-enhancing composition according to any one of claims 1 to 6 in the preparation of health food products.

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