An in vitro culture device for intestinal flora

Abstract

The invention discloses an in vitro culture device for intestinal flora, which relates to the technical field of flora culture, and comprises: a culture chamber, which is a closed chamber structure; a temperature-controlled water body, which is pre-placed at the inner bottom of the culture chamber and is temperature-controlled by an external circulating temperature-control device; a driving component, which has a driving end, and the driving end is used to drive a culture dish to rotate around its central vertical axis; a visual sensor, which is arranged at the inner top of the culture chamber and is used to monitor the growth of intestinal flora in the culture dish; and a stirring head control component, which has a rotating control end, and the rotating control end is connected to a stirring head, and the stirring head can be sent to the culture dish through the rotating control end, or taken out of the culture dish and rotated to achieve giving way to the visual sensor; the culture dish is driven to rotate by the driving component, and the stirring head is sent to the culture dish by the stirring head control component at the same time, so as to achieve simulation of intestinal peristalsis.

Description

Technical Field

[0001] The invention relates to the technical field of flora culture, in particular to an in vitro culture device for intestinal flora. Background Art

[0002] In the field of intestinal microbiology, the study of intestinal flora plays a vital role in understanding human health, disease prevention and treatment. With the advancement of science and technology, in vitro culture technology has become one of the important means to study intestinal flora. However, the existing in vitro culture equipment still has some shortcomings in design and function, which limits its application effect in intestinal flora research.

[0003] For example, intestinal motility is an important part of intestinal function, which has an important impact on food digestion, nutrient absorption, and the distribution and metabolism of intestinal flora. However, existing in vitro culture equipment often cannot simulate intestinal motility effects, and therefore cannot provide specific intestinal motility effects, which makes it impossible to study the effects of other parameters on intestinal flora under specific intestinal motility effects.

[0004] Therefore, it is necessary to provide an in vitro culture device for intestinal flora to solve the above problems. Summary of the invention

[0005] To achieve the above object, the present invention provides the following technical solution: an in vitro culture device for intestinal flora, comprising:

[0006] The culture chamber is a closed chamber structure with a one-way air outlet;

[0007] A temperature-controlled water body is pre-placed at the inner bottom of the culture bin and is temperature-controlled by an external circulation temperature-control device;

[0008] A drive assembly having a drive end for driving the culture dish to rotate about its central vertical axis;

[0009] a visual sensor disposed on the inner top of the culture chamber and used to monitor the growth of intestinal flora in the culture dish; and a stirring head control component having a rotating control end, the rotating control end being connected to a stirring head, through which the stirring head can be sent into the culture dish, or taken out of the culture dish and rotated, so as to make way for the visual sensor;

[0010] The culture dish is driven to rotate by the driving component, and the stirring head is sent into the culture dish by the stirring head control component, so as to realize the simulation of intestinal peristalsis.

[0011] Further, as a preference, the stirring head control assembly comprises:

[0012] A lifter having a lift end, and the lifter is located above the outer side of the culture bin;

[0013] A first rod body, which is fixed below the lifting end; and a second rod body, which is rotatably disposed below the first rod body, and the second rod body is sealed and slidably penetrates the top of the culture bin;

[0014] The second rod body serves as a rotation control end of the stirring head control assembly;

[0015] And the stirring head is bent.

[0016] Further, preferably, a spring is provided between the second rod body and the first rod body, and the spring has a tendency to drive the stirring head to reset; when the stirring head is reset, its downward projection is partially mapped in the culture dish;

[0017] A second magnetic block is fixed on the second rod body, and a first magnetic block is fixed on the inner top of the culture chamber; when the stirring head is reset, the first magnetic block and the second magnetic block are misaligned, and when the second magnetic block moves upward and approaches the first magnetic block, the second magnetic block and the first magnetic block are magnetically attracted to each other, and the second magnetic block rotates until it matches the first magnetic block.

[0018] Further, preferably, the driving assembly comprises:

[0019] A ring seat, which is fixed on the inner wall of the culture chamber;

[0020] A limiting ring, which is rotatably arranged in the ring groove on the upper part of the ring seat;

[0021] A ferrule is located on the inner side of the ring seat, the ferrule is connected to the limiting ring, and the ferrule serves as the driving end of the driving assembly.

[0022] Further, as a preference, a friction wheel is rotatably provided at the bottom of the ring seat, the friction wheel contacts the ferrule, and a power unit is also provided on one side of the ring seat, the power unit is used to drive the friction wheel.

[0023] Further, as a preference, a controller is further provided on one side of the culture chamber, and an intestinal peristalsis model is provided in the controller; based on the Monod equation and the exponential decay function, the intestinal peristalsis model is constructed as follows:

[0024]

[0025] Wherein, RPM is the stirring speed, and the stirring speed is the rotation speed of the culture dish;

[0026] Δt is the stirring duration;

[0027] θ is the stirring contact area;

[0028] RPM opt is the optimal stirring speed;

[0029] Δt opt is the optimal stirring duration;

[0030] θ opt is the optimal stirring contact area;

[0031] RPM range is the effective range of stirring speed;

[0032] Δt range is the effective range of stirring duration;

[0033] θ range It is the effective range of stirring contact area.

[0034] Furthermore, preferably, in the intestinal peristalsis model,

[0035]

[0036] Where, d is the diameter of the stirring head;

[0037] Δz is the contact depth between the stirring head and the intestinal flora in the culture dish.

[0038] Further, preferably, the intestinal peristalsis model includes:

[0039] Obtaining historical intestinal peristalsis data information, wherein the historical intestinal peristalsis data information includes intestinal peristalsis data information of each calibration coefficient category;

[0040] Based on the intestinal motility data information and intestinal motility models of each calibration coefficient category, data fitting is performed and an intestinal motility model library is constructed;

[0041] Determine the intestinal motility calibration coefficient according to the intestinal flora category;

[0042] Based on the intestinal peristalsis calibration coefficient, the corresponding intestinal peristalsis model is called from the intestinal peristalsis model library.

[0043] Further, preferably, the culture chamber is supplied with gas from a nitrogen tank until gas outflow is detected at the one-way air outlet.

[0044] Further, preferably, a splash plate is placed on the surface of the temperature-controlled water body, and a through hole is opened on the splash plate for making way for the culture dish, and the density of the splash plate is lower than that of the temperature-controlled water body.

[0045] Compared with the prior art, the present invention provides an in vitro culture device for intestinal flora, which has the following beneficial effects:

[0046] In the present invention, an intestinal motility model library can be established based on historical intestinal motility data information and intestinal motility models. When studying a specific intestinal flora, the corresponding intestinal motility model can be quickly called to facilitate the analysis of the influence of stirring speed, stirring duration, and stirring contact area on the intestinal motility effect and the influence on the in vitro culture of intestinal flora; based on this, the influence of other parameters on the in vitro culture of intestinal flora can also be studied under a specific intestinal motility effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 It is a schematic diagram of the overall structure of an in vitro culture device for intestinal flora;

[0048] Figure 2 A schematic diagram of the structure of a driving component in an in vitro culture device for intestinal flora;

[0049] Figure 3 A schematic diagram of the structure of a stirring head control component in an in vitro culture device for intestinal flora Figure 1 ;

[0050] Figure 4 A schematic diagram of the structure of a stirring head control component in an in vitro culture device for intestinal flora Figure 2 ;

[0051] In the figure: 1. culture chamber; 2. temperature-controlled water body; 3. splash plate; 4. drive assembly; 5. culture dish; 6. visual sensor; 7. stirring head control assembly; 8. nitrogen tank; 9. first magnetic block; 10. stirring head; 11. controller; 41. ferrule; 42. ring seat; 43. power unit; 44. friction wheel; 45. limit ring; 71. lifter; 72. first rod body; 73. second rod body; 74. spring; 75. second magnetic block. DETAILED DESCRIPTION

[0052] The terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned description of the drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequential order. It should be understood that the terms used in this way can be interchangeable under appropriate circumstances, which is merely a way of distinguishing the objects of the same attributes when describing them in the embodiments of the present application. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, so that the process, method, system, product or equipment comprising a series of units need not be limited to those units, but may include other units that are not clearly listed or inherent to these processes, methods, products or equipment.

[0053] Example: Please refer to Figure 1-Figure 4 In an embodiment of the present invention, there is provided an in vitro culture device for intestinal flora, comprising:

[0054] The culture chamber 1 is a closed chamber structure, which provides a stable environment for cultivating intestinal flora, and the culture chamber 1 has a one-way air outlet, which is designed to control the gas exchange in the culture chamber and maintain the stability of the culture environment;

[0055] The temperature-controlled water body 2 is pre-placed at the inner bottom of the culture chamber 1 and is temperature-controlled by an external circulation temperature-control device. The temperature-controlled water body 2 is used to maintain the temperature in the culture chamber 1. The temperature in the culture chamber 1 can be accurately controlled by the external circulation temperature-control device to ensure that the intestinal flora grows at a suitable temperature.

[0056] A driving assembly 4 having a driving end for driving the culture dish 5 to rotate around its central vertical axis;

[0057] a visual sensor 6 disposed on the inner top of the culture chamber 1 and used to monitor the growth of the intestinal flora in the culture dish 5; and a stirring head control component 7 having a rotating control end, the rotating control end being connected to a stirring head 10, through which the stirring head 10 can be sent into the culture dish 5, or taken out of the culture dish 5 and rotated, so as to make way for the visual sensor 6;

[0058] The culture dish 5 is driven to rotate by the driving component 4, and the stirring head 10 is sent into the culture dish 5 by the stirring head control component 7, so as to realize the simulation of intestinal peristalsis.

[0059] In this embodiment, the working principle of the whole device is to drive the culture dish 5 to rotate through the driving component 4, and at the same time use the stirring head control component 7 to send the stirring head 10 into the culture dish, so as to simulate the environment of intestinal peristalsis. This simulated environment may help to more accurately simulate the growth environment of intestinal flora and improve the effect of in vitro culture. At the same time, through the real-time monitoring of the visual sensor 6, the growth of the flora can be understood in time, providing strong support for research work.

[0060] It should also be explained that mechanical stirring can simulate intestinal peristalsis, mainly because the two have some similarities in physical processes and effects.

[0061] First of all, intestinal peristalsis is a movement produced by the contraction and relaxation of intestinal smooth muscle, which helps food and digestive juices mix, propel and absorb in the intestine. This movement is a complex, rhythmic and continuous process that has an important impact on the growth and metabolism of intestinal flora.

[0062] Mechanical stirring is a process of mixing materials evenly by rotation or other means. In in vitro culture equipment used to simulate intestinal peristalsis, a mechanical stirrer can rotate the culture dish or stirring head through a drive component, thereby producing a similar mixing and propulsion effect in the culture dish. This stirring action helps simulate the flow and mixing environment in the intestine and promotes uniform contact and interaction between the intestinal flora and the culture medium.

[0063] Secondly, the intensity and frequency of mechanical stirring can be adjusted as needed to simulate intestinal peristalsis of different intensities and frequencies. This flexibility allows the mechanical stirrer to more accurately simulate various conditions of intestinal peristalsis, including normal peristalsis, hyperactive peristalsis, and attenuated peristalsis.

[0064] In addition, mechanical stirring can also be combined with other technical means to simulate other characteristics of intestinal peristalsis, such as temperature changes, pH changes, etc. These factors may affect the growth and metabolism of intestinal flora. Therefore, by comprehensively simulating these factors, the intestinal peristalsis environment can be more comprehensively simulated, and the accuracy and reliability of in vitro culture can be improved.

[0065] If the drive assembly 4 is configured as two, then there are two culture dishes 5 in the culture chamber 1 at the same time (in Figure 1 The drive assembly 4 corresponding to the culture dish 5 on the right side is located at the back of the culture chamber 1 and is therefore not shown) for comparative experiments.

[0066] Based on the above situation (there are two culture dishes 5 in the culture chamber 1 at the same time), in this embodiment, the stirring head control component 7 includes:

[0067] A lifter 71 having a lift end, and the lifter 71 is located above the outer side of the culture chamber 1;

[0068] A first rod 72, which is fixed below the lifting end; and a second rod 73, which is rotatably disposed below the first rod 72, and the second rod 73 is sealed and slidably penetrates the top of the culture chamber 1;

[0069] The second rod body 73 serves as the rotation control end of the stirring head control assembly 7;

[0070] The stirring head 10 is bent, more specifically, Figure 3 The stirring head 10 is a bent symmetrical structure.

[0071] The lifter 71 includes but is not limited to an electric telescopic rod, a hydraulic rod, and a cylinder;

[0072] The design of the entire stirring head control assembly 7 ensures that the stirring head 10 can accurately and flexibly enter and exit the culture dish 5, and also ensures the sealing of the culture chamber 1 and the rotation control capability of the stirring head 10. These features enable the device to more accurately simulate the intestinal peristalsis environment and improve the accuracy and reliability of in vitro culture.

[0073] In addition, a spring 74 is provided between the second rod 73 and the first rod 72, and the spring 74 has a tendency to drive the stirring head 10 to reset; when the stirring head 10 is reset, its downward projection is partially reflected in the culture dish 5;

[0074] A second magnetic block 75 is fixed on the second rod body 73, and a first magnetic block 9 is fixed on the inner top of the culture chamber 1; when the stirring head 10 is reset, the first magnetic block 9 and the second magnetic block 75 are misaligned, and when the second magnetic block 75 moves upward and approaches the first magnetic block 9, the second magnetic block 75 and the first magnetic block 9 are magnetically attracted to each other, and the second magnetic block 75 rotates until it matches the first magnetic block 9.

[0075] It should be explained that when stirring is required, the lifter 71 drives the stirring head 10 to descend through the first rod body 72 and the second rod body 73 .

[0076] Stirring process: The stirring head 10 is partially inserted into the culture dish 5, and at the same time, the culture dish 5 starts to rotate to achieve a stirring effect.

[0077] Stirring head reset: After stirring is completed, the spring 74 drives the stirring head 10 to move upward. As the second magnetic block 75 moves upward and approaches the first magnetic block 9, the two are magnetically attracted to each other, causing the second magnetic block 75 to rotate until it matches the first magnetic block 9.

[0078] This ensures that the stirring head 10 remains in the correct position when not in operation (that is, the downward projection of the stirring head 10 is not reflected in the culture dish 5 at all), so as to overcome the problem of interference with the visual sensor 6. This ensures that the visual sensor 6 can effectively monitor the intestinal flora.

[0079] In this embodiment, the driving component 4 includes:

[0080] A ring seat 42, which is fixed on the inner wall of the culture chamber 1;

[0081] A limiting ring 45 is rotatably disposed in a ring groove on an upper portion of the ring seat 42;

[0082] The ferrule 41 is located on the inner side of the ring seat 42 . The ferrule 41 is connected to the limiting ring 45 . The ferrule 41 serves as the driving end of the driving assembly 4 .

[0083] A friction wheel 44 is rotatably provided at the bottom of the ring seat 42 . The friction wheel 44 contacts the ferrule 41 . A power unit 43 is also provided at one side of the ring seat 42 . The power unit 43 is used to drive the friction wheel 44 .

[0084] In this embodiment, the ring seat 42 is fixed on the inner wall of the culture chamber 1, providing a stable installation foundation for the ferrule 41. The limiting ring 45 is rotatably set in the ring groove of the ring seat 42 and is connected to the ferrule 41 to ensure the stability and accuracy of the ferrule 41. The ferrule 41 is located on the inner side of the ring seat 42 and serves as the driving end of the driving assembly. It directly contacts or is connected to the culture dish 5. The friction wheel 44 is located at the bottom of the ring seat 42 and contacts the outer side of the ferrule 41, waiting for the drive of the power unit 43.

[0085] During the startup phase, after receiving the startup command, the power unit 43 starts working and drives the friction wheel 44 to rotate. The rotation of the friction wheel 44 acts on the ferrule 41 through friction, causing the ferrule 41 to also start rotating. Since the ferrule 41 is connected to or in direct contact with the culture dish 5, the culture dish 5 also starts rotating following the rotation of the ferrule 41.

[0086] In this embodiment, the culture chamber 1 is supplied with gas by a nitrogen tank 8 until gas outflow is detected at the one-way air outlet.

[0087] Before the nitrogen tank 8 starts to supply gas, it is necessary to ensure that the valve on the pipeline connecting the nitrogen tank 8 and the culture chamber 1 is open. Once the valve is opened, the nitrogen in the nitrogen tank 8 will flow into the culture chamber 1 through the pipeline under the action of the pressure difference. In this process, it may be necessary to adjust the opening of the valve to control the flow rate and flow rate of the nitrogen. There will be one or more one-way air outlets at a certain position of the culture chamber 1 (usually the top or side). The design of these air outlets allows the gas to flow only from the inside of the culture chamber 1 to the outside, preventing external air from entering the culture chamber 1. During the gas supply process, it is possible to detect whether there is gas flowing out of the one-way air outlet.

[0088] This is usually achieved by detecting the gas flow or pressure change at the air outlet. By detecting whether there is gas flowing out of the one-way air outlet, it can be determined whether the sealing of the culture chamber 1 is good. If there is no gas flowing out of the air outlet, it may mean that there is a gas leak in the culture chamber 1, which will affect the stability of the gas environment inside the culture chamber 1.

[0089] By detecting the gas flow or pressure changes at the air outlet, it is possible to monitor whether the gas supply process is normal and whether the gas supply is completed.

[0090] In addition, nitrogen is an inert gas with stable chemical properties and will not react chemically with the culture, so it is suitable as the gas environment of the culture chamber 1. Therefore, the use of nitrogen gas supply can remove oxygen and other gases in the culture chamber 1 that may interfere with the culture process, providing a more stable and pure culture environment for the culture. 。

[0091] In this embodiment, a splash plate 3 is placed on the surface of the temperature-controlled water body 2 . The splash plate 3 is provided with through holes for making way for the culture dish 5 . The density of the splash plate 3 is lower than that of the temperature-controlled water body 2 .

[0092] The density of the splash plate 3 is lower than that of the temperature-controlled water body 2, which enables the splash plate 3 to float on the surface of the temperature-controlled water body 2. This design enables the splash plate 3 to move with the fluctuation of the temperature-controlled water body 2, and always maintains the protective effect on the culture dish 5.

[0093] It is worth mentioning that the splash plate 3 floats on the surface of the temperature-controlled water body 2, forming a barrier that can effectively reduce the direct contact between the temperature-controlled water body 2 and the upper environment, thereby reducing heat loss. This is very critical for maintaining the temperature stability of the temperature-controlled water body 2, especially in a culture environment that requires precise temperature control. Due to the presence of the splash plate 3, the air flow on the surface of the temperature-controlled water body 2 will be restricted to a certain extent, further reducing the temperature fluctuation caused by air flow. This stable temperature environment is crucial for the growth and reproduction of cultures.

[0094] In this embodiment, a controller 11 is further provided on one side of the culture chamber 1, and an intestinal peristalsis model is provided in the controller 11; based on the Monod equation and the exponential decay function, the intestinal peristalsis model is constructed as:

[0095]

[0096] Wherein, RPM is the stirring speed, and the stirring speed is the rotation speed of the culture dish 5;

[0097] Δt is the stirring duration;

[0098] θ is the stirring contact area;

[0099] RPM opt is the optimal stirring speed;

[0100] Δt opt is the optimal stirring duration;

[0101] Δ opt is the optimal stirring contact area;

[0102] RPM range is the effective range of stirring speed;

[0103] Δt range is the effective range of stirring duration;

[0104] θ range It is the effective range of stirring contact area.

[0105] In the intestinal motility model,

[0106]

[0107] Wherein, d is the diameter of the stirring head 10;

[0108] Δz is the contact depth between the stirring head 10 and the intestinal flora in the culture dish 5 .

[0109] In addition, before the intestinal peristalsis model is called, the steps include: obtaining historical intestinal peristalsis data information, wherein the historical intestinal peristalsis data information includes intestinal peristalsis data information of each calibration coefficient category;

[0110] Based on the intestinal motility data information and intestinal motility models of each calibration coefficient category, data fitting is performed and an intestinal motility model library is constructed;

[0111] Determine the intestinal motility calibration coefficient according to the intestinal flora category;

[0112] Based on the intestinal peristalsis calibration coefficient, the corresponding intestinal peristalsis model is called from the intestinal peristalsis model library.

[0113] It needs to be explained that the system or researchers need to collect data information about intestinal motility in the past. This data may come from experiments, observations, medical records or other relevant sources.

[0114] These data information usually include intestinal motility data of multiple calibration coefficient categories. The calibration coefficient categories refer to different intestinal states, different physiological conditions, different individuals or groups, etc.

[0115] After obtaining historical data, these data are used to fit the intestinal motility model. The process of data fitting is to adjust the parameters of the model so that the results predicted by the model are as close as possible to the actual observed data.

[0116] Since there are multiple calibration coefficient categories (i.e., different intestinal conditions or states), a separate model needs to be fitted for each category and stored in a intestinal motility model library.

[0117] In addition, the intestinal flora category refers to the types of microorganisms in the intestine. Different intestinal flora may have different effects on intestinal motility.

[0118] An intestinal motility calibration coefficient is determined according to the current intestinal flora category. This coefficient is used to indicate which model or models in the model library best matches the current intestinal condition.

[0119] After the intestinal motility calibration coefficient is determined, the model that best matches the current intestinal conditions can be called from the intestinal motility model library.

[0120] This called model will be used to predict or analyze the current intestinal motility.

[0121] In other words, an intestinal motility model library can be established based on historical intestinal motility data information and intestinal motility models. When studying specific intestinal flora, the corresponding intestinal motility model can be quickly called to facilitate the analysis of the effects of stirring speed, stirring duration, and stirring contact area on intestinal motility and on the in vitro culture of intestinal flora; based on this, the effects of other parameters on the in vitro culture of intestinal flora can also be studied under specific intestinal motility effects.

[0122] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An in vitro culture device for intestinal flora, characterized in that: include: The culture chamber (1) is a closed chamber structure and has a one-way air outlet; A temperature-controlled water body (2) is pre-placed at the inner bottom of the culture bin (1) and is temperature-controlled by an external circulation temperature control device; A driving assembly (4) having a driving end, the driving end being used to drive the culture dish (5) to rotate around its central vertical axis; a visual sensor (6) arranged on the inner top of the culture chamber (1) and used to monitor the growth of intestinal flora in the culture dish (5); and a stirring head control component (7) having a rotating control end, the rotating control end being connected to a stirring head (10), and the stirring head (10) can be sent into the culture dish (5) or taken out of the culture dish (5) and rotated through the rotating control end to achieve the purpose of making way for the visual sensor (6); The culture dish (5) is driven to rotate by the driving component (4), and the stirring head (10) is sent into the culture dish (5) by the stirring head control component (7), so as to realize the simulation of intestinal peristalsis; The stirring head control assembly (7) comprises: A lifter (71) having a lift end, and the lifter (71) is located above the outer side of the culture bin (1); A first rod (72) fixed below the lifting end; and a second rod (73) rotatably disposed below the first rod (72), and the second rod (73) sealingly slides through the top of the culture bin (1); The second rod body (73) serves as a rotation control end of the stirring head control assembly (7); The stirring head (10) is in a bent shape; A spring (74) is provided between the second rod (73) and the first rod (72), and the spring (74) has a tendency to drive the stirring head (10) to reset; when the stirring head (10) is reset, its downward projection is partially reflected in the culture dish (5); A second magnetic block (75) is fixed on the second rod body (73), and a first magnetic block (9) is fixed on the inner top of the culture chamber (1); when the stirring head (10) is reset, the first magnetic block (9) and the second magnetic block (75) are misaligned, and when the second magnetic block (75) moves upward and approaches the first magnetic block (9), the second magnetic block (75) and the first magnetic block (9) are magnetically attracted to each other, and the second magnetic block (75) rotates until it matches the first magnetic block (9); The driving assembly (4) comprises: A ring seat (42) fixed on the inner wall of the culture chamber (1); A limiting ring (45) is rotatably disposed in a ring groove on the upper portion of the ring seat (42); A ferrule (41) located on the inner side of the ring seat (42), the ferrule (41) being connected to the limiting ring (45), and the ferrule (41) serving as the driving end of the driving assembly (4); A friction wheel (44) is rotatably arranged at the bottom of the ring seat (42), and the friction wheel (44) contacts the ferrule (41). A power unit (43) is also arranged on one side of the ring seat (42), and the power unit (43) is used to drive the friction wheel (44).

2. The in vitro culture device for intestinal flora according to claim 1, characterized in that: A controller (11) is also provided on one side of the culture chamber (1), and an intestinal peristalsis model is provided in the controller (11); based on the Monod equation and the exponential decay function, the intestinal peristalsis model is constructed as follows: Wherein, RPM is the stirring speed, and the stirring speed is the rotation speed of the culture dish (5); Δt is the stirring duration; θ is the stirring contact area; RPM opt is the optimal stirring speed; Δt opt is the optimal stirring duration; Δ opt is the optimal stirring contact area; RPM range is the effective range of stirring speed; Δt range is the effective range of stirring duration; θ range It is the effective range of stirring contact area.

3. The in vitro culture device for intestinal flora according to claim 2, characterized in that: In the intestinal motility model, Wherein, d is the diameter of the stirring head (10); Δz is the contact depth between the stirring head (10) and the intestinal flora in the culture dish (5).

4. The in vitro culture device for intestinal flora according to claim 2, characterized in that: The intestinal motility model includes the following before being called: Obtaining historical intestinal peristalsis data information, wherein the historical intestinal peristalsis data information includes intestinal peristalsis data information of each calibration coefficient category; Based on the intestinal motility data information and intestinal motility models of each calibration coefficient category, data fitting is performed and an intestinal motility model library is constructed; Determine the intestinal motility calibration coefficient according to the intestinal flora category; Based on the intestinal peristalsis calibration coefficient, the corresponding intestinal peristalsis model is called from the intestinal peristalsis model library.

5. The in vitro culture device for intestinal flora according to claim 1, characterized in that: The culture chamber (1) is supplied with gas from a nitrogen tank (8) until gas outflow is detected at the one-way air outlet.

6. The in vitro culture device for intestinal flora according to claim 1, characterized in that: A splash plate (3) is placed on the surface of the temperature-controlled water body (2), and a through hole is provided on the splash plate (3) for making way for the culture dish (5), and the density of the splash plate (3) is lower than that of the temperature-controlled water body (2).

Images