A specific combination of phosphate-solubilizing bacterial strains and a carrier and co-cultivation method for the same

CN118685313BActive Publication Date: 2026-07-03ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG FORESTRY UNIVERSITY
Filing Date
2024-06-26
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

The problem of available phosphorus in soil is severe in tropical and subtropical acidic red soils. The application of existing inorganic phosphate fertilizers reduces biodiversity and has low utilization efficiency. It is necessary to explore green and efficient ways to utilize soil phosphorus.

Method used

A co-culture method was adopted using a specific combination of phosphate-solubilizing bacteria strains (Burkholderia cepacia, Burkholderia pyrrolizin, Enterobacter aerogenes, and Bacillus subtilis) with a mixture of waste peanut shells and sugarcane bagasse as a carrier. The culture conditions were optimized to maintain the activity and proliferation of the strains.

Benefits of technology

It effectively solves the problems of easy inactivation of phosphorus-solubilizing microorganisms in liquids and high transportation costs, realizes efficient utilization of soil phosphorus, reduces raw material costs and maintains strain activity.

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Abstract

The application discloses a specific phosphorus-dissolving bacterial strain combination, a carrier for the strain combination and a co-cultivation method, and the strain combination is composed of Burkholderia cenocepacia, Burkholderia pyrrocinia, Enterobacter aerogenes and Bacillus subtilis, and the carrier is a mixture of discarded peanut shells and sugarcane residues. The application determines the dominant carrier suitable for the growth of the specific strain combination, meets the strain type and the number of living bacteria of the microbial agent product stipulated by the country, and effectively solves the problems of easy inactivation of the phosphorus-dissolving microbial strain in liquid, short preservation time and high transportation cost through the optimal cultivation process system of the specific phosphorus-dissolving microbial strain combination on the carrier. The strain activity and proliferation are maintained through the fermentation of the strain on the solid carrier, so as to meet the market demand for the phosphorus-dissolving microbial agent product.
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