Feed additive for delaying or improving reproductive function aging of laying hens in the late egg-laying period and application thereof

Abstract

The present invention provides a feed additive for delaying or improving the aging of reproductive function of laying hens in the late egg-laying period and its application, belonging to the technical field of feed additives. The feed additive comprises the following raw materials in parts by weight: 10 to 15 parts of chlorogenic acid, 10 to 15 parts of curcumin, 4 to 8 parts of astragalus polysaccharide, 1 to 3 parts of soy isoflavones, 0.1 to 0.5 parts of microbial agents, 1 to 3 parts of glucose oxidase, 2 to 3 parts of complex vitamins, 5 to 8 parts of complex amino acids and 1 to 3 parts of medical stone powder. The feed additive provided by the present invention can improve the reproductive hormone indexes of laying hens, significantly increase the number of follicles, reduce the atresia rate, improve the ovarian function of laying hens, and delay the aging of reproductive function of laying hens in the late egg-laying period.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, in particular to a feed additive for delaying or improving reproductive function aging of laying hens in the late egg-laying period and application thereof. Background Art

[0002] Studies have shown that laying hens generally enter the egg-laying period at 18 to 20 weeks of age, reach the peak of egg-laying at 24 to 25 weeks of age, and the egg-laying rate is about 95%. Then the egg-laying rate begins to decline, dropping to about 90% at 45 weeks of age, and to 70% at 95 weeks of age. It can be seen that the egg-laying capacity of laying hens will drop sharply in the late egg-laying period. This is because after laying hens enter the late egg-laying period, their immunity gradually decreases, their resistance decreases, and the structure and function of various tissues and organs have been damaged and degraded to varying degrees. There are problems such as a large number of follicles consumed, an increase in the rate of follicle atresia, a decrease in reproductive hormone levels, and an increase in oxidative stress. This has caused the ovarian function, egg-laying capacity and egg quality of laying hens to decline seriously, and even increased the egg-laying rate and egg breakage rate of deformed eggs, which has seriously affected the production efficiency of the farm.

[0003] Therefore, it is hoped to provide a technical solution that can delay or improve the reproductive function aging of laying hens in the late egg-laying period and restore the ovarian function of laying hens in the late egg-laying period. Summary of the invention

[0004] The purpose of the present invention is to provide a method for delaying or improving the reproductive function aging of laying hens in the late egg-laying period, so as to restore the ovarian function of laying hens in the late egg-laying period.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a feed additive for delaying or improving reproductive function aging of laying hens in the late egg-laying period. The feed additive comprises the following raw materials in parts by weight: 10 to 15 parts of chlorogenic acid, 10 to 15 parts of curcumin, 4 to 8 parts of astragalus polysaccharide, 1 to 3 parts of soybean isoflavones, 0.1 to 0.5 parts of microbial agent, 1 to 3 parts of glucose oxidase, 2 to 3 parts of complex vitamins, 5 to 8 parts of complex amino acids and 1 to 3 parts of medical stone powder.

[0007] Preferably, the chlorogenic acid is prepared by the following preparation method:

[0008] (1) Take Eucommia ulmoides leaves, grind them into 50-150 meshes, mix them with 40-60% ethanol solution by volume for 2-3 times, combine the extracts, and then concentrate the extracts to 1 / 5-1 / 10 of the original volume, separate the solid and liquid, and take the clear liquid to obtain a crude extract;

[0009] (2) The crude extract is saturatedly adsorbed by NKA-9 macroporous adsorption resin, and eluted with water, 30-40% ethanol solution, and 60-70% ethanol solution in sequence, and the 30-40% ethanol solution eluate and the 60-70% ethanol solution eluate are collected and combined, and concentrated to 1 / 5-1 / 10 of the total volume, and dried to obtain the chlorogenic acid.

[0010] Preferably, in step (1), the mixing ratio of Eucommia ulmoides leaves and ethanol solution is: 1 g: 5-10 mL;

[0011] The extraction is ultrasonic extraction with a frequency of 18-24 Hz, a temperature of 60-80° C. and an extraction time of 0.5-2 h.

[0012] Preferably, the vitamin complex comprises 0.8 to 1.2 parts by weight of vitamin A, 0.8 to 1.2 parts by weight of vitamin C, 0.5 to 0.8 parts by weight of vitamin E and 0.1 to 0.5 parts by weight of folic acid.

[0013] Preferably, the microbial agent is Enterococcus faecium and / or Bacillus subtilis, and the total effective viable count is (1-3)×10 6 CFU / g.

[0014] Preferably, the glucose oxidase has an enzyme activity of 800 to 1200 U / g.

[0015] Preferably, the complex amino acids are two or more of taurine, tryptophan, lysine and DL-methionine.

[0016] The present invention provides a method for preparing the above-mentioned feed additive, comprising the following steps: taking chlorogenic acid, curcumin, astragalus polysaccharide, soybean isoflavones, microbial agents, glucose oxidase, complex vitamins and complex amino acids, mixing, stirring at 25-30° C. for 10-15 minutes, then adding medical stone powder, and stirring at 30-35° C. for 20-30 minutes to obtain the feed additive.

[0017] The invention provides application of the feed additive in delaying or improving the aging of reproductive function of laying hens.

[0018] The present invention also provides application of the feed additive in improving the ovarian function of poultry and livestock.

[0019] The feed additive provided by the present invention is composed of a variety of natural plant extracts, such as chlorogenic acid from eucommia extract, soy isoflavones from soybean extract and curcumin from turmeric extract, etc. The ingredients are green and safe, and are supplemented with Enterococcus faecium, glucose oxidase and various vitamins and amino acids. The components work synergistically with each other and work together to improve the reproductive hormone indicators of laying hens, increase the number of follicles, reduce the follicle atresia rate, improve the ovarian function of laying hens, delay ovarian aging, and improve the aging problem of the reproductive function of laying hens in the later stage of egg laying. DETAILED DESCRIPTION

[0020] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0021] Example 1

[0022] (1) Take Eucommia ulmoides leaves, crush them into 100 mesh, mix them with a 60% volume fraction ethanol solution at a ratio of 1 g:10 mL, perform ultrasonic extraction 3 times (ultrasonic frequency is 20 Hz, temperature is 80°C, and each extraction time is 1 h), combine the extracts, and then concentrate the extracts to 1 / 8 of the original volume, filter, and take the clear liquid to obtain a crude extract (the mass content of chlorogenic acid in the crude extract is 10.87%);

[0023] (2) The crude extract was saturatedly adsorbed by NKA-9 macroporous adsorption resin, and eluted with water, 30% ethanol solution, and 60% ethanol solution in sequence (the flow rate of the adsorption extract was 0.5 retention volume / hour, and the flow rate during desorption was 2 retention volumes / hour). The 30-40% ethanol solution eluate and the 60-70% ethanol solution eluate were collected and combined, concentrated to 1 / 5 of the total volume, and spray-dried (inlet air temperature 170°C, outlet air temperature 70°C) to obtain the chlorogenic acid.

[0024] Example 2

[0025] A feed additive for delaying or improving reproductive function aging of laying hens in the late egg-laying period, the preparation method of which is as follows: take 10 parts of chlorogenic acid prepared in Example 1, 10 parts of curcumin, 4 parts of astragalus polysaccharide, 1 part of soy isoflavones, 0.5 parts of Enterococcus faecium, 1 part of glucose oxidase (enzyme activity is 1000U / g), 0.8 parts by weight of vitamin A, 1.2 parts by weight of vitamin C, 0.5 parts by weight of vitamin E, 0.5 parts by weight of folic acid, 2 parts of tryptophan and 3 parts of lysine, mix, stir at 30°C for 15 minutes, then add 2 parts of medical stone powder, and stir at 35°C for 30 minutes to obtain the feed additive.

[0026] Example 3

[0027] A feed additive for delaying or improving the reproductive function aging of laying hens in the late egg-laying period, the preparation method comprising: taking 15 parts of chlorogenic acid prepared in Example 1, 15 parts of curcumin, 8 parts of astragalus polysaccharide, 2 parts of soybean isoflavones, and a microbial agent (Enterococcus faecium and Bacillus subtilis are composed of an effective viable count of 1:1, and the total effective viable count is 1×10 6 CFU / g), 0.5 parts of glucose oxidase (enzyme activity is 1000U / g), 1.5 parts of glucose oxidase (enzyme activity is 1000U / g), 1.2 parts by weight of vitamin A, 0.8 parts by weight of vitamin C, 0.7 parts by weight of vitamin E, 0.3 parts by weight of folic acid and 1 part of taurine, 1 part of tryptophan, 3 parts of lysine and 1 part of DL-methionine are mixed, stirred at 30°C for 15 minutes, and then 2 parts of medical stone powder are added, and then stirred at 35°C for 30 minutes to obtain the feed additive.

[0028] Example 4

[0029] A feed additive for delaying or improving reproductive function aging of laying hens in the late egg-laying period, the preparation method comprising: taking 15 parts of chlorogenic acid prepared in Example 1, 12 parts of curcumin, 5 parts of astragalus polysaccharide, 3 parts of soybean isoflavones, and a microbial agent (Enterococcus faecium and Bacillus subtilis are composed of an effective viable count of 2:1, and a total effective viable count of 3×10 6 CFU / g), 0.5 parts of glucose oxidase (enzyme activity is 1000U / g), 2.5 parts of glucose oxidase (enzyme activity is 1000U / g), 1 part by weight of vitamin A, 1 part by weight of vitamin C, 0.5 parts by weight of vitamin E and 0.5 parts by weight of folic acid, 2 parts of taurine, 2 parts of tryptophan, 2 parts of lysine and 2 parts of DL-methionine, mix, stir at 30°C for 15 minutes, then add 3 parts of medical stone powder, and stir at 35°C for 30 minutes to prepare the feed additive.

[0030] Comparative Example 1

[0031] Different from Example 4, in this example, betaine is used in an equal amount to replace chlorogenic acid.

[0032] Comparative Example 2

[0033] Different from Example 4, in this example, an equal amount of Lactobacillus plantarum was used to replace Enterococcus faecium.

[0034] Comparative Example 3

[0035] Take 20 parts of curcumin, 10 parts of astragalus polysaccharide, 3 parts of soybean isoflavones, and microbial agents (Enterococcus faecium and Bacillus subtilis are composed of 2:1 effective viable bacteria, with a total effective viable bacteria count of 3×10 6CFU / g), 0.5 parts of glucose oxidase (enzyme activity is 1000U / g), 2.5 parts of glucose oxidase (enzyme activity is 1000U / g), 1 part by weight of vitamin A, 1 part by weight of vitamin C, 0.5 parts by weight of vitamin E and 0.5 parts by weight of folic acid, 2 parts of taurine, 2 parts of tryptophan, 2 parts of lysine and 2 parts of DL-methionine, mix, stir at 30°C for 15 minutes, then add 3 parts of medical stone powder, and stir at 35°C for 30 minutes to prepare the feed additive.

[0036] Test example

[0037] Fifty 50-week-old Beijing-red laying hens with similar weight from the same batch and the same early feeding conditions were randomly selected in the same farm and divided into 5 groups, each with 10, namely, Example 4 group, Comparative Example 1-3 groups, and a group without feed additives (control), wherein the control group was fed with a basic diet, and the basic diet can refer to the "New Edition of Jinghong No. 1 Laying Hen Feeding and Management Manual". Example 4 group and Comparative Example 1-3 groups were fed with a basic diet, and 1 kg of feed additives was added to each ton of basic diet. The experimental laying hen house is an ordinary large-scale laying hen house (window type), with two layers of cages in the chicken house, and 2 chickens in each cage. The room temperature of the chicken house is controlled at about 25°C, and the light duration is 16h. Free access to food and water. The experiment was completed after 15 weeks, and the feed was cut off for 12 hours after the experiment.

[0038] 1. Three laying hens were randomly selected from each group, wing vein blood was collected, and the contents of estradiol (E2), growth hormone GH / (ng / mL), luteinizing hormone LH / (ng / mL), follicle stimulating hormone FSH / (ng / mL), and progesterone (P4) in serum were determined using ELISA kits. The operation method was carried out according to the instructions of the kit. The test results are shown in Table 1:

[0039] Table 1 Hormone changes in laying hens in different experimental groups

[0040] E2(pg / mL) GH / (ng / mL) LH / (ng / mL) FSH / (ng / mL) P4 (ng / mL) Example 4 660.45 0.51 6.84 7.58 0.94 Comparative Example 1 630.44 0.44 6.43 7.38 0.81 Comparative Example 2 642.14 0.47 6.59 7.41 0.86 Comparative Example 3 625.21 0.43 6.54 7.32 0.76 Comparison 512.23 0.37 6.35 6.91 0.70

[0041] As can be seen from Table 1, compared with the control, the present invention can increase the estradiol and progesterone content in the serum of laying hens, and has an improvement effect on other hormone indicators in the serum, and the improvement effect is better than that of comparative examples 1 to 3, indicating that the feed additive raw materials provided by the present invention are synergistic with each other and have more advantages than other raw materials of the same type.

[0042] 2. Randomly select 3 laying hens, kill them, remove the ovaries, quickly separate the graded follicles (diameter>8mm), small yellow follicles (diameter 4-8mm) and large white follicles (diameter 2-4mm) with ophthalmic forceps, record their numbers, and then quickly put the ovaries into Bouin's fixative for making slices and HE staining, refer to the method of Li Yinghui et al. (Li Yinghui, Wang Linxian. The regulatory effect of PMSG and GnRH analogs on chicken follicle atresia [J]), observe the slices under a light microscope, record the number of normal and atretic primary follicles and secondary follicles on each slice, and calculate the atresia rate of secondary follicles, atresia rate (%) = number of atretic follicles / number of normal follicles × 100. The counting results are shown in Tables 2 and 3:

[0043] Table 2 Changes in the number of follicles of different grades in laying hens in different experimental groups (pieces)

[0044] Grade follicles Small yellow follicle Large white follicle Primary follicle Secondary follicles Example 4 7.21 19.21 17.31 184.21 161.58 Comparative Example 1 6.16 17.85 15.89 164.38 144.45 Comparative Example 2 6.41 18.44 16.24 171.25 156.24 Comparative Example 3 5.95 17.51 15.31 158.98 142.14 Comparison 5.32 16.88 12.13 131.12 121.54

[0045] Table 3 Changes in the atresia rate of follicles of different grades in laying hens in different experimental groups (%)

[0046]

[0047]

[0048] As shown in Tables 2 and 3, compared with the control and comparative examples 1 to 3, the feed additive provided by the present invention can significantly increase the number of graded follicles, small yellow follicles, large white follicles and primary follicles, and significantly reduce the atresia rate of small yellow follicles, large white follicles and secondary follicles. In summary, it can be seen that the feed additive provided by the present invention can improve the reproductive hormone indexes of laying hens, significantly increase the number of follicles, and reduce the atresia rate, delay the decline of ovarian function of laying hens, and restore the ovarian function of laying hens in the late egg-laying period.

[0049] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A feed additive for delaying or improving the aging of reproductive function of laying hens in the late egg-laying period, characterized in that: The invention is composed of the following raw materials in parts by weight: 10-15 parts of chlorogenic acid, 10-15 parts of curcumin, 4-8 parts of astragalus polysaccharide, 1-3 parts of soybean isoflavones, 0.1-0.5 parts of microbial agent, 1-3 parts of glucose oxidase, 2-3 parts of multivitamins, 5-8 parts of multiamino acids and 1-3 parts of medical stone powder; The multivitamin comprises 0.8 to 1.2 parts by weight of vitamin A, 0.8 to 1.2 parts by weight of vitamin C, 0.5 to 0.8 parts by weight of vitamin E and 0.1 to 0.5 parts by weight of folic acid; The microbial agents are Enterococcus faecium and Bacillus subtilis, and the total effective viable count is (1-3)×10 6 CFU / g; The complex amino acids are two or more of taurine, tryptophan, lysine and DL-methionine.

2. The feed additive according to claim 1, characterized in that The chlorogenic acid is prepared by the following preparation method: (1) Take Eucommia ulmoides leaves, crush them into 50-150 meshes, mix them with 40-60% ethanol solution by volume for 2-3 times, combine the extracts, and then concentrate the extracts to 1 / 5-1 / 10 of the original volume, separate the solid and liquid, and take the clear liquid to obtain a crude extract; (2) The crude extract is saturatedly adsorbed by NKA-9 macroporous adsorption resin, and eluted with water, 30-40% ethanol solution, and 60-70% ethanol solution in sequence, the 30-40% ethanol solution eluate and the 60-70% ethanol solution eluate are collected and combined, concentrated to 1 / 5-1 / 10 of the total volume, and dried to obtain the chlorogenic acid.

3. The feed additive according to claim 2, characterized in that: In step (1), the mixing ratio of Eucommia ulmoides leaves and ethanol solution is: 1 g: 5-10 mL; The extraction is ultrasonic extraction with a frequency of 18-24 Hz, a temperature of 60-80° C., and an extraction time of 0.5-2 h.

4. The feed additive according to claim 1, characterized in that The enzymatic activity of the glucose oxidase is 800-1200 U / g.

5. The method for preparing the feed additive according to any one of claims 1 to 4, characterized in that: The following steps are involved: Take chlorogenic acid, curcumin, astragalus polysaccharide, soy isoflavones, microbial agents, glucose oxidase, complex vitamins and complex amino acids, mix them, stir at 25-30° C. for 10-15 minutes, then add medical stone powder, and then stir at 30-35° C. for 20-30 minutes to obtain the feed additive.

6. Use of the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5 in delaying or improving the aging of reproductive function of laying hens.

7. Use of the feed additive according to any one of claims 1 to 4 or the feed additive prepared by the preparation method according to claim 5 in improving the ovarian function of poultry and livestock.

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