A cysteamine coated product and its production process

Abstract

The present invention proposes a cysteamine coated product and its production process, and belongs to the technical field of cysteamine. The following steps are included: S1. Using high-voltage electrostatic spraying method, using modified hyaluronic acid and sodium alginate as wall materials, and cysteamine hydrochloride as core material, a first microcapsule is prepared; S2. Using freeze drying method, hydrophobic modified starch and whey protein as wall materials, using the first microcapsule as core material, a second microcapsule is prepared; S3. Using chitosan gelatin as wall material, and using the second microcapsule as core material to prepare a cysteamine coated product. The cysteamine coated product prepared by the present invention has good acid resistance, can target active cysteamine to the intestinal tract, avoid gastric acid to destroy cysteamine, improve the stability of cysteamine, promote the growth and development of poultry and livestock, improve the utilization rate of feed, improve the quality of carcass meat, and has good heat resistance and moisture resistance stability.

Description

Technical Field

[0001] The invention relates to the technical field of cysteamine, and in particular to a cysteamine coated product and a production process thereof. Background Art

[0002] Cysteamine (CS), also known as β-mercaptoethylamine, is an intermediate metabolite of cysteine ​​and a component of coenzyme A. It contains active sulfhydryl and hydrogen groups and has multiple physiological effects. It has an inhibitory regulatory effect on animal growth hormone, thyroxine, insulin and other metabolites. It can reduce the content of somatostatin in livestock and poultry, indirectly increase the concentration of growth hormone, thereby promoting the growth of livestock and poultry, and can improve feed utilization, improve carcass quality, and effectively promote the absorption and utilization of calcium and phosphorus by animal bodies, reduce the phosphorus content in livestock and poultry feces, and is beneficial to environmental protection. However, cysteamine is very easy to oxidize into disulfide in the air and lose its effectiveness, and it will irritate the stomach wall in the stomach and cause severe gastric ulcers. Therefore, it cannot be used in large-scale industrial production. At the same time, it also has the disadvantages of low melting point (70.2-70.7℃), easy deliquescence, active chemical properties (easy to oxidize, easy to complex, etc.), making it difficult to be used in large-scale industrial production. Summary of the invention

[0003] The purpose of the present invention is to provide a cysteamine coated product and a production process thereof, which has good acid resistance, can target the delivery of active cysteamine to the intestine, avoid the destruction of cysteamine by gastric acid, improve the stability of cysteamine, promote the growth and development of poultry and livestock, improve the utilization rate of feed, improve the quality of carcass meat, have good heat and moisture resistance stability, and have broad application prospects.

[0004] The technical solution of the present invention is achieved in this way:

[0005] The present invention provides a production process of a cysteamine coated product, comprising the following steps:

[0006] S1. A first microcapsule was prepared by high-voltage electrostatic spraying with modified hyaluronic acid and sodium alginate as wall materials and cysteamine hydrochloride as core material;

[0007] S2. A second microcapsule was prepared by freeze drying, using hydrophobically modified starch and whey protein as the wall material and the first microcapsule as the core material;

[0008] S3. Prepare a cysteamine coated product using chitosan-gelatin as the wall material and the second microcapsule as the core material.

[0009] As a further improvement of the present invention, the preparation method of the modified hyaluronic acid is as follows: hyaluronic acid is added to water, activated with N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl)carbodiimide, silk fibroin peptide is added, stirred for reaction, ethanol is added for precipitation, filtered, washed, and dried to obtain modified hyaluronic acid.

[0010] As a further improvement of the present invention, the mass ratio of the hyaluronic acid, N-hydroxysuccinimide, 1-ethyl-(3-dimethylaminopropyl)carbodiimide and silk fibroin peptide is 15-20:11-13:18-20:7-10, the temperature of the activation reaction is 0-4°C, the time is 20-30min, the temperature of the stirring reaction is 35-45°C, the time is 5-7h, and ethanol is added to the system to reach an ethanol content of 75-85wt%, and the precipitation is 2-4h.

[0011] As a further improvement of the present invention, the preparation method of the hydrophobically modified starch is as follows: long-chain fatty acids and starch are added to N,N-dimethylformamide, a catalyst is added, and under the protection of an inert gas, the mixture is heated and stirred for reaction, filtered, washed, and recrystallized from ethanol to obtain the hydrophobically modified starch.

[0012] As a further improvement of the present invention, the long-chain fatty acid is selected from at least one of octanoic acid, nonanoic acid, decanoic acid, undecanoic acid, dodecanoic acid, tetradecanoic acid, hexadecanoic acid, and octadecanoic acid, the mass ratio of the long-chain fatty acid to starch is 5-6:12-15, the catalyst is concentrated sulfuric acid, the addition amount is 2-3wt% of the total mass of the system, the temperature of the heating and stirring reaction is 120-130°C, and the time is 4-6h.

[0013] As a further improvement of the present invention, the following steps are included:

[0014] S1. Sodium alginate, cysteamine hydrochloride, and lecithin are added to water, added to fish oil, homogenized, calcium chloride solution is added dropwise, solidified at room temperature, centrifuged, washed, dried, the obtained microcapsules are added to water, modified hyaluronic acid is added, stirred and mixed, high-voltage electrostatic spraying is performed, and freeze-dried to obtain a first microcapsule;

[0015] S2. Add the hydrophobically modified starch and whey protein to water, heat and stir to dissolve, add the first microcapsules and emulsifier prepared in step S1, stir and disperse evenly, add dropwise to the fish oil, emulsify, homogenize, centrifuge, wash, and freeze-dry to obtain the second microcapsules;

[0016] S3. Dissolve carboxymethyl chitosan and gelatin in water, add the second microcapsule obtained in step S2, homogenize, adjust the pH value of the solution to the first pH value, stir to react, cool, adjust the pH value of the solution to the second pH value, stir to react, let stand, centrifuge, wash, freeze-dry, and obtain a cysteamine coated product.

[0017] As a further improvement of the present invention, the mass ratio of sodium alginate, cysteamine hydrochloride, lecithin and modified hyaluronic acid in step S1 is 12-15:10-12:0.5-1:13-17, the homogenization condition is homogenization at 22-25 MPa for 5-10 min, the high-voltage electrostatic spraying condition is a flow rate of 0.5-2.5 mL / h, the distance between the receiving plate and the spray port of the syringe needle is 15-20 cm, the diameter of the spray port is 0.5 mm, and the solution is pushed by an axial flow injection pump at a voltage of 10-25 kV.

[0018] As a further improvement of the present invention, the mass ratio of the hydrophobically modified starch, whey protein, the first microcapsule and the emulsifier in step S2 is 15-20:7-10:17-22:1-2, the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60 and Tween-80, the heating temperature is 40-50°C, and the homogenization condition is homogenization at 25-30MPa for 10-15min.

[0019] As a further improvement of the present invention, the mass ratio of carboxymethyl chitosan, gelatin and the second microcapsule in step S3 is 22-25:12-16:25-30, the first pH value is 4.2-4.5, the second pH value is 5.9-6.2, the temperature is lowered to 10-15°C, the standing time is 12-15h, and the homogenization condition is homogenization at 45-49MPa for 5-7min.

[0020] The present invention further protects a cysteamine coated product prepared by the above production process.

[0021] The present invention has the following beneficial effects:

[0022] The active thiol of cysteamine can open the disulfide bond in the somatostatin molecule, destroy the molecular structure and biological activity of somatostatin, effectively reduce the level of somatostatin, relieve the inhibitory effect of somatostatin on the body's anabolism and digestive function, promote the secretion of endogenous growth hormone and various digestive enzymes, enhance the body's anabolism and digestion and absorption of nutrients, significantly promote the rapid growth of animals and increase the lean meat rate of carcasses. At the same time, the active thiol of cysteamine can convert cystine into cysteine, synthesize glutathione with glutamic acid and glycine, increase the concentration of glutathione, an important antioxidant in animals, and play the role of antioxidant and immune enhancement through glutathione, effectively improve meat quality and color, and improve the water content of meat lines, so as to obtain better quality livestock and poultry products.

[0023] The present invention adopts a high-voltage electrostatic spray method to prepare microcapsules, and uses an external electric field to control the force of the liquid or solution to be sprayed. When the liquid passes through the external electric field, the polymer droplets are simultaneously subjected to the combined effects of their own gravity, electric field force, surface tension, attraction and repulsion between molecules, and the like, and the same kind of charges are continuously accumulated. When the charge aggregation reaches a limit, the electrostatic force of the charge accumulation is greater than the surface tension of the fluid, and a jet liquid column is generated. The liquid column is atomized into tiny droplets through the action of force, and the prepared microcapsules have the characteristics of regular capsules, good controllability of particle size, and easy control of parameters. The present invention adopts this method to prepare the first microcapsule, and cysteamine is embedded in the first layer. The size of the microcapsule is controllable, and modified hyaluronic acid is used as the embedding wall material. The hyaluronic acid and silk protein peptide are condensed and modified, and the film-forming property is good, the embedding size is small, and the uniformity and smoothness are high, which is convenient for subsequent embedding.

[0024] The present invention uses hydrophobically modified starch and whey protein as wall materials. The hydrophobically modified starch makes the polysaccharide amphiphilic, so a thick and tough interface layer can be formed on the interface of the emulsion, showing good emulsification properties and emulsification stability. At the same time, the wall material has pH responsiveness, does not degrade under acidic conditions, and degrades under alkaline conditions, which is convenient for intestinal transportation of active components.

[0025] The composite coacervation microcapsule technology uses oppositely charged polymer materials as wall materials, disperses the core material in the wall material solution, adjusts the pH, forms a solidified film on the surface of the core material through electrostatic self-assembly between opposite charges, and uses the phase separation phenomenon after the solubility change to obtain microcapsules. The present invention selects carboxymethyl chitosan and gelatin as wall materials, which have the characteristics of high load and high temperature resistance, and have significant advantages in the sustained and controlled release of the core material, thereby preparing the cysteamine coated product of the present invention.

[0026] The cysteamine coated product prepared by the invention has good acid resistance, can deliver active cysteamine to the intestine in a targeted manner, avoids the destruction of cysteamine by gastric acid, improves the stability of cysteamine, promotes the growth and development of poultry and livestock, improves the utilization rate of feed, improves the quality of carcass meat, has good heat resistance and moisture resistance stability, and has broad application prospects. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Hyaluronic acid was purchased from Jiangsu Jiujia Biotechnology Co., Ltd. Starch was industrial-grade corn starch, product standard GB / T 8885, purchased from Shandong Anquan Chemical Technology Co., Ltd.

[0029] Preparation Example 1 Preparation of modified hyaluronic acid

[0030] The method is as follows: add 15 parts by weight of hyaluronic acid to 500 parts by weight of water, add 11 parts by weight of N-hydroxysuccinimide and 18 parts by weight of 1-ethyl-(3-dimethylaminopropyl)carbodiimide, activate at 0°C for 20 minutes, add 7 parts by weight of silk protein peptide, heat to 35°C, stir to react for 5 hours, add ethanol until the ethanol content of the system is 75wt%, precipitate for 2 hours, filter, wash, and dry to obtain modified hyaluronic acid.

[0031] Preparation Example 2 Preparation of modified hyaluronic acid

[0032] The method is as follows: add 20 parts by weight of hyaluronic acid to 500 parts by weight of water, add 13 parts by weight of N-hydroxysuccinimide and 20 parts by weight of 1-ethyl-(3-dimethylaminopropyl)carbodiimide, activate at 4°C for 30 minutes, add 10 parts by weight of silk protein peptide, heat to 45°C, stir and react for 7 hours, add ethanol until the ethanol content of the system is 85wt%, precipitate for 4 hours, filter, wash, and dry to obtain modified hyaluronic acid.

[0033] Preparation Example 3 Preparation of modified hyaluronic acid

[0034] The method is as follows: add 17 parts by weight of hyaluronic acid to 500 parts by weight of water, add 12 parts by weight of N-hydroxysuccinimide and 19 parts by weight of 1-ethyl-(3-dimethylaminopropyl)carbodiimide, activate at 2°C for 25 minutes, add 8.5 parts by weight of silk protein peptide, heat to 40°C, stir to react for 6 hours, add ethanol until the ethanol content of the system is 80wt%, precipitate for 3 hours, filter, wash, and dry to obtain modified hyaluronic acid.

[0035] Preparation Example 4 Preparation of hydrophobically modified starch

[0036] The method is as follows: 5 parts by weight of octanoic acid and 12 parts by weight of starch are added to 200 parts by weight of N,N-dimethylformamide, and concentrated sulfuric acid is added in an amount of 2wt% of the total mass of the system. Under nitrogen protection, the mixture is heated to 120°C, stirred for reaction for 4 hours, filtered, washed, and recrystallized from ethanol to obtain a hydrophobically modified starch.

[0037] Preparation Example 5 Preparation of hydrophobically modified starch

[0038] The method is as follows: add 6 parts by weight of capric acid and 15 parts by weight of starch to 200 parts by weight of N,N-dimethylformamide, add concentrated sulfuric acid in an amount of 3wt% of the total mass of the system, heat to 130°C under nitrogen protection, stir to react for 6 hours, filter, wash, and recrystallize with ethanol to obtain hydrophobically modified starch.

[0039] Preparation Example 6 Preparation of hydrophobically modified starch

[0040] The method is as follows: 5.5 parts by weight of dodecanoic acid and 13.5 parts by weight of starch are added to 200 parts by weight of N,N-dimethylformamide, and concentrated sulfuric acid is added in an amount of 2.5wt% of the total mass of the system. Under nitrogen protection, the mixture is heated to 125°C, stirred for reaction for 5 hours, filtered, washed, and recrystallized with ethanol to obtain a hydrophobically modified starch.

[0041] Example 1

[0042] This embodiment provides a production process of a cysteamine coated product, comprising the following steps:

[0043] S1. 12 parts by weight of sodium alginate, 10 parts by weight of cysteamine hydrochloride, and 0.5 parts by weight of lecithin are added to 200 parts by weight of water, added dropwise to 500 parts by weight of fish oil, homogenized at 22 MPa for 5 min, 20 parts by weight of 3 wt% calcium chloride solution are added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried, the obtained microcapsules are added to 500 parts by weight of water, 13 parts by weight of the modified hyaluronic acid obtained in Preparation Example 1 are added, stirred and mixed evenly, high-voltage electrostatic spraying is performed, and freeze-dried to obtain a first microcapsule;

[0044] The conditions of the high-voltage electrostatic spray are as follows: the flow rate is 0.5 mL / h, the distance between the receiving plate and the spray port of the syringe needle is 15 cm, the diameter of the spray port is 0.5 mm, the solution is pushed by an axial flow syringe pump, and the voltage is 10 kV;

[0045] S2. 15 parts by weight of the hydrophobically modified starch prepared in Preparation Example 4 and 7 parts by weight of whey protein were added to 200 parts by weight of water, heated to a temperature of 40°C, stirred to dissolve, 17 parts by weight of the first microcapsule prepared in step S1 and 1 part by weight of an emulsifier Tween-20 were added, stirred and dispersed uniformly, and added dropwise to 500 parts by weight of fish oil, emulsified at 10000 r / min min, homogenized at 25 MPa for 10 min, centrifuged, washed, and freeze-dried to obtain a second microcapsule;

[0046] S3. Dissolve 22 parts by weight of carboxymethyl chitosan and 12 parts by weight of gelatin in 500 parts by weight of water, add 25 parts by weight of the second microcapsule obtained in step S2, homogenize at 45 MPa for 5 minutes, adjust the pH value of the solution to 4.2, stir and react for 30 minutes, cool to 10°C, adjust the pH value of the solution to 5.9, stir and react for 30 minutes, let stand for 12 hours, centrifuge, wash, and freeze-dry to obtain a cysteamine coated product.

[0047] Example 2

[0048] This embodiment provides a production process of a cysteamine coated product, comprising the following steps:

[0049] S1. 15 parts by weight of sodium alginate, 12 parts by weight of cysteamine hydrochloride, and 1 part by weight of lecithin are added to 200 parts by weight of water, added dropwise to 500 parts by weight of fish oil, homogenized at 25 MPa for 10 min, 20 parts by weight of 3 wt% calcium chloride solution are added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried, the obtained microcapsules are added to 500 parts by weight of water, 17 parts by weight of the modified hyaluronic acid obtained in Preparation Example 2 are added, stirred and mixed evenly, high-voltage electrostatic spraying is performed, and freeze-dried to obtain a first microcapsule;

[0050] The conditions of the high-voltage electrostatic spray are as follows: the flow rate is 2.5 mL / h, the distance between the receiving plate and the spray port of the syringe needle is 20 cm, the diameter of the spray port is 0.5 mm, the solution is pushed by an axial flow syringe pump, and the voltage is 25 kV;

[0051] S2. 20 parts by weight of the hydrophobic modified starch prepared in Preparation Example 5 and 10 parts by weight of whey protein were added to 200 parts by weight of water, heated to a temperature of 50°C, stirred to dissolve, 22 parts by weight of the first microcapsule prepared in step S1 and 2 parts by weight of emulsifier Tween-40 were added, stirred and dispersed uniformly, and added dropwise to 500 parts by weight of fish oil, emulsified at 10000 r / min for min, homogenized at 30 MPa for 15 min, centrifuged, washed, and freeze-dried to obtain a second microcapsule;

[0052] S3. Dissolve 25 parts by weight of carboxymethyl chitosan and 16 parts by weight of gelatin in 500 parts by weight of water, add 30 parts by weight of the second microcapsule obtained in step S2, homogenize at 49 MPa for 7 minutes, adjust the pH value of the solution to 4.5, stir and react for 30 minutes, cool to 15°C, adjust the pH value of the solution to 6.2, stir and react for 30 minutes, let stand for 15 hours, centrifuge, wash, and freeze-dry to obtain a cysteamine-coated product.

[0053] Example 3

[0054] This embodiment provides a production process of a cysteamine coated product, comprising the following steps:

[0055] S1. 14 parts by weight of sodium alginate, 11 parts by weight of cysteamine hydrochloride, and 0.7 parts by weight of lecithin are added to 200 parts by weight of water, added dropwise to 500 parts by weight of fish oil, homogenized at 23.5 MPa for 7 min, 20 parts by weight of 3 wt% calcium chloride solution are added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried, the obtained microcapsules are added to 500 parts by weight of water, 15 parts by weight of the modified hyaluronic acid obtained in Preparation Example 3 are added, stirred and mixed evenly, high-voltage electrostatic spraying is performed, and freeze-dried to obtain the first microcapsules;

[0056] The conditions of the high-voltage electrostatic spray are as follows: the flow rate is 1.5 mL / h, the distance between the receiving plate and the spray port of the syringe needle is 17 cm, the diameter of the spray port is 0.5 mm, the solution is pushed by an axial flow syringe pump, and the voltage is 19 kV;

[0057] S2. 17 parts by weight of the hydrophobically modified starch prepared in Preparation Example 6 and 8.5 parts by weight of whey protein were added to 200 parts by weight of water, heated to a temperature of 45 ° C, stirred to dissolve, 20 parts by weight of the first microcapsule prepared in step S1 and 1.5 parts by weight of emulsifier Tween-80 were added, stirred and dispersed uniformly, and added dropwise to 500 parts by weight of fish oil, emulsified at 10000 r / min min, homogenized at 27 MPa for 12 min, centrifuged, washed, and freeze-dried to obtain a second microcapsule;

[0058] S3. Dissolve 23 parts by weight of carboxymethyl chitosan and 14 parts by weight of gelatin in 500 parts by weight of water, add 27 parts by weight of the second microcapsule obtained in step S2, homogenize at 47 MPa for 6 minutes, adjust the pH value of the solution to 4.4, stir and react for 30 minutes, cool to 12°C, adjust the pH value of the solution to 6.0, stir and react for 30 minutes, let stand for 13 hours, centrifuge, wash, and freeze-dry to obtain a cysteamine-coated product.

[0059] Comparative Example 1

[0060] Compared with Example 3, the difference is that the modified hyaluronic acid in step S1 is replaced by hyaluronic acid of equal mass.

[0061] Comparative Example 2

[0062] Compared with Example 3, the difference is that step S1 is not performed.

[0063] Comparative Example 3

[0064] Compared with Example 3, the difference is that the hydrophobically modified starch in step S2 is replaced by starch of equal mass.

[0065] Comparative Example 4

[0066] Compared with Example 3, the difference is that step S2 is not performed.

[0067] Comparative Example 5

[0068] Compared with Example 3, the difference is that step S3 is not performed.

[0069] Test Example 1

[0070] 270 healthy fattening pigs with an average initial weight of (67.82±1.09) kg were selected and randomly divided into 9 groups, with 3 replicates in each group and 10 pigs (5 males and 5 females) in each replicate. The pre-test period was 5 days and the main test period was 40 days. The control group was fed a basal diet, and the experimental group was added with 100 mg / kg of the cysteamine coated product prepared in Example 1-3 or Comparative Example 1-5 to the basal diet. The pigs were raised in pens, immunized with normal procedures, raised according to routine management, and the pig houses were routinely disinfected. The experimental pigs were free to eat and drink water.

[0071] The formula of the basic diet is as follows (mass percentage): corn 63.5wt%, soybean meal 20wt%, wheat bran 8wt%, peanut cake 4.5wt%, calcium carbonate 1.4wt%, calcium bicarbonate 1.3wt%, salt 0.3wt%, premix 1wt%, wherein each kilogram of premix contains: vitamin A 6200IU, vitamin D 3700IU, vitamin E 88IU, vitamin K (menadione sodium bisulfite complex) 4.4mg, vitamin B 28.8mg, pantothenic acid 24.2mg, niacin (nicotinic acid) 33mg, choline 330mg; copper 15mg (CuSO4·5H2O), zinc 105mg (ZnSO4·7H2O), iron 135mg (FeSO4·H2O), manganese 40mg (MnSO4·5H2O), selenium 0.15mg (Na2SeO3·5H2O), iodine 0.3mg (KI).

[0072] After the experiment, one boar and one sow with the closest average weight were selected from each repetition and fasted for 12 hours. 5 mL of anterior vena cava blood was collected, centrifuged, and the upper serum was taken and stored at -20°C. The selected fattening pigs were killed by electroshock bleeding, and the abdominal cavity was opened to separate the liver and duodenum. About 100 g of liver tissue was taken from the middle of the left hepatic lobule, frozen in liquid nitrogen, and stored at -80°C for later use. The contents of the duodenum were collected in an Eppendorf tube, immediately placed in a liquid nitrogen tank, and then transferred to -80°C for low temperature storage.

[0073] 1. Growth performance

[0074] The body weight of each replicate was measured at the beginning and end of the experiment, and the feed consumption of each replicate was recorded every day. The average daily gain (ADG), average daily feed intake (ADFI) and feed / gain ratio (F / G) were calculated.

[0075] The results are shown in Table 1.

[0076] Table 1

[0077]

[0078] It can be seen from the above table that the cysteamine coated products prepared in Examples 1-3 of the present invention can significantly promote the growth of finishing pigs and improve the feed consumption to weight gain ratio.

[0079] 2. Serum immune indicators Serum immune indicators IgA, IgG, IgM

[0080] The corresponding kits were used to determine the levels of serum immune indicators IgA, IgG, and IgM.

[0081] The results are shown in Table 2.

[0082] Table 2

[0083] Group IgA content (g / L) IgG content (g / L) IgM content (g / L) Control group 0.09 2.26 0.12 Example 1 0.10 2.41 0.10 Example 2 0.08 2.39 0.11 Example 3 0.11 2.43 0.13 Comparative Example 1 0.09 2.33 0.11 Comparative Example 2 0.10 2.31 0.12 Comparative Example 3 0.11 2.34 0.10 Comparative Example 4 0.12 2.30 0.13 Comparative Example 5 0.13 2.28 0.13

[0084] It can be seen from the above table that the cysteamine coated products prepared in Examples 1-3 of the present invention can significantly increase the IgG content, but have no significant promoting effect on IgA and IgM.

[0085] 3. Serum antioxidant index serum

[0086] The total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activities, and malondialdehyde (MDA) content were determined using corresponding kits.

[0087] The results are shown in Table 3.

[0088] Table 3

[0089]

[0090] It can be seen from the above table that the cysteamine coated products prepared in Examples 1-3 of the present invention can significantly improve the total antioxidant capacity, total superoxide dismutase, and glutathione peroxidase activities, and reduce the malondialdehyde content.

[0091] 4. Digestive enzyme activity index

[0092] Weigh part of the duodenal contents, add pre-cooled physiological saline with a volume of 9 times the weight of the contents, homogenize, centrifuge, take the supernatant, and store it at -80°C for testing. Use the corresponding kits to determine the activities of total protease, total amylase and total lipase. The results are shown in Table 4.

[0093] Table 4

[0094]

[0095]

[0096] It can be seen from the above table that the cysteamine coated products prepared in Examples 1-3 of the present invention can significantly improve the activities of total protease, total amylase and total lipase.

[0097] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A production process for a cysteamine coated product, characterized in that: The following steps are involved: S1. Add sodium alginate, cysteamine hydrochloride and lecithin into water, add into fish oil, homogenize, add calcium chloride solution dropwise, solidify at room temperature, centrifuge, wash, dry, add the obtained microcapsules into water, add modified hyaluronic acid, stir and mix evenly, spray with high-voltage electrostatic spray, freeze-dry, and obtain the first microcapsules; the mass ratio of sodium alginate, cysteamine hydrochloride, lecithin and modified hyaluronic acid is 12-15:10-12:0.5-1:13-17, the homogenization condition is homogenization at 22-25MPa for 5-10min, the high-voltage electrostatic spray condition is a flow rate of 0.5-2.5mL / h, the distance between the receiving plate and the spray port of the syringe needle is 15-20cm, the diameter of the spray port is 0.5mm, and the solution is driven by an axial flow injection pump with a voltage of 10-25kV; S2. Add hydrophobically modified starch and whey protein into water, heat and stir to dissolve, add the first microcapsule and emulsifier prepared in step S1, stir and disperse evenly, add dropwise into fish oil, emulsify, homogenize, centrifuge, wash, freeze-dry, and prepare second microcapsules; the mass ratio of the hydrophobically modified starch, whey protein, first microcapsule and emulsifier is 15-20:7-10:17-22:1-2, the emulsifier is selected from at least one of Tween-20, Tween-40, Tween-60 and Tween-80, the heating temperature is 40-50°C, and the homogenization condition is 25-30MPa for 10-15min; S3. Dissolve carboxymethyl chitosan and gelatin in water, add the second microcapsule obtained in step S2, homogenize, adjust the pH value of the solution to the first pH value, stir to react, cool, adjust the pH value of the solution to the second pH value, stir to react, stand, centrifuge, wash, freeze-dry, and obtain a cysteamine coated product; the mass ratio of the carboxymethyl chitosan, gelatin, and the second microcapsule is 22-25:12-16:25-30, the first pH value is 4.2-4.5, the second pH value is 5.9-6.2, the temperature is lowered to 10-15°C, the standing time is 12-15h, and the homogenization condition is 5-7min at 45-49MPa.

2. The production process of the cysteamine coated product according to claim 1, characterized in that: The preparation method of the modified hyaluronic acid is as follows: hyaluronic acid is added into water, activated by N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl)carbodiimide, silk fibroin peptide is added, stirred for reaction, ethanol is added for precipitation, filtered, washed, and dried to obtain the modified hyaluronic acid.

3. The production process of the cysteamine coated product according to claim 2, characterized in that: The mass ratio of the hyaluronic acid, N-hydroxysuccinimide, 1-ethyl-(3-dimethylaminopropyl)carbodiimide and silk fibroin peptide is 15-20:11-13:18-20:7-10, the temperature of the activation reaction is 0-4°C, the time is 20-30min, the temperature of the stirring reaction is 35-45°C, the time is 5-7h, ethanol is added until the ethanol content of the system is 75-85wt%, and the precipitation is 2-4h.

4. The production process of the cysteamine coated product according to claim 1, characterized in that: The preparation method of the hydrophobically modified starch is as follows: adding long-chain fatty acids and starch to N,N-dimethylformamide, adding a catalyst, heating and stirring the reaction under the protection of an inert gas, filtering, washing, and ethanol recrystallization to obtain the hydrophobically modified starch; the long-chain fatty acid is selected from at least one of octanoic acid, nonanoic acid, decanoic acid, undecanoic acid, dodecanoic acid, tetradecanoic acid, hexadecanoic acid, and octadecanoic acid, the mass ratio of the long-chain fatty acid to the starch is 5-6:12-15, the catalyst is concentrated sulfuric acid, the addition amount is 2-3wt% of the total mass of the system, the temperature of the heating and stirring reaction is 120-130°C, and the time is 4-6h.

5. A cysteamine coated product prepared by the production process according to any one of claims 1 to 4.

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