A complete pet canned food for improving immunity of pets and a preparation method thereof
Complete pet food, formulated with specific ratios of amino acids, peptides, probiotics, and traditional Chinese medicine extracts, addresses the shortcomings of canned pet food in terms of nutrition and immunity enhancement, thus promoting the healthy growth of pets, especially puppies.
Patent Information
- Application Number
- CN202410966671.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-18
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2044-07-18
AI Technical Summary
While existing canned pet food meets the daily nutritional needs of pets, it is difficult to effectively improve their immunity, especially for weaned puppies, who are more susceptible to disease.
Made with specific proportions of amino acids, peptides, probiotics, traditional Chinese medicine extracts, and mineral components, and prepared through specific enzymatic hydrolysis and mixing processes, this complete pet food provides comprehensive nutrition and immune-boosting effects.
It can improve a pet's immunity and digestive ability, reduce the risk of allergies, and promote healthy growth, especially the healthy and safe growth of puppies.
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of pet food, and particularly relates to a full-value pet can which can improve the immunity of pets and a preparation method thereof. BACKGROUND
[0002] Pet food is a diet specially designed for pets, aiming to provide pets with necessary nutrients and energy. There are various types of pet food on the current pet market, among which pet cans are a common type of pet food, and are favored by pets and pet owners due to their high water content (usually more than 70%) and rich meat quality.
[0003] Pet cans can be divided into three categories according to their functionality: snack cans, main food cans and prescription cans. Among them, main food cans are highly praised due to the diversification of their formula ingredients and comprehensive nutrition. These cans usually add a variety of nutrients to the minced meat paste, making full-value pet main food cans, which are rich in nutrients and relatively balanced in collocation, and can meet the daily nutritional needs of pets, and are an important source of main food for pets.
[0004] However, although pet owners tend to choose pet food that is rich in nutrients and balanced in collocation for pets, in actual life, pets may still face various physical function problems. For example, newly weaned puppies have an immature digestive system, poor absorption capacity and weak constitution, resulting in low resistance and being easily affected by diseases; pets recovering from serious illness have not yet recovered their immune system, and at this time, feeding them with cans on the market not only has poor absorption capacity, but also causes a burden to the body.
[0005] Therefore, pet owners urgently need a pet food that can meet the daily nutritional needs of pets while further improving the immunity of pets. SUMMARY
[0006] The purpose of the present application is to provide a full-value pet can which can improve the immunity of pets, enhance the constitution of pets, and at the same time, supplement a variety of nutrients for pets, so that pets, especially newly weaned puppies, can grow healthily and safely. In order to achieve the above purpose, the present application provides the following technical solutions:
[0007] A full-value pet can which can improve the immunity of pets, the preparation raw materials of which include, by weight percentage, 70% to 85% of meat, 1% to 5% of supplementary additives, and the balance to 100% of water.
[0008] The meat includes, by weight percentage, 82% to 90% of muscle, 3% to 10% of bone-in meat, and 5% to 15% of blood-in viscera.
[0009] The meat source is not limited, such as muscle can be derived from duck, beef, mutton, chicken, turkey meat, etc.; bone meat can be derived from chicken clavicle, etc.; blood viscera can be derived from rabbit liver, chicken heart, chicken liver, etc.
[0010] The supplementary additive includes, by weight, 0.5-1 parts of amino acid, 1-2 parts of peptide substance, 0.2-0.8 parts of probiotic, 1.2-1.8 parts of traditional Chinese medicine extract, 0.1-0.5 parts of mineral component, and 1-1.5 parts of compound vitamin.
[0011] Preferably, the amino acid is a combination of L-cysteine, L-arginine, taurine and lysine.
[0012] Preferably, the mass ratio of L-cysteine, L-arginine, taurine and lysine is (0.5-1):(0.8-1.2):(1-1.5):1; further preferably, 0.8:1:1.2:1.
[0013] In some preferred embodiments, L-cysteine, L-arginine, taurine and lysine with a mass ratio of (0.5-1):(0.8-1.2):(1-1.5):1 are selected as the amino acid added to the can, which can improve the immunity and digestive capacity of pets, especially pets in the early childhood. This can be because when the pet children are just weaned, the digestive system is not mature and the intestinal function is not perfect, so the immunity and the absorption capacity of nutrients are poor, especially the synthetic capacity of nutritional amino acid of pet cats is lower than that of dogs, so they are more susceptible to diseases or die of hunger. The applicant accidentally found that there is a synergistic effect among the amino acids in a specific ratio, which can make up for the deficiency of some amino acids in pet cans and ensure that pets obtain comprehensive nutrition. Moreover, lysine, L-cysteine and L-arginine have a synergistic effect on protein metabolism and nitrogen excretion, which can help pets to convert the excess nitrogen in the body into urea and excrete it out of the body, thereby preventing the accumulation of ammonia in the blood and causing poisoning, helping to maintain the health of pets and ensuring the healthy growth of pets. Taurine and lysine not only improve vision and enhance eye protection, but also protect the stomach and liver and enhance immunity.
[0014] The preparation method of the peptide substance includes the following steps:
[0015] S1, drying and grinding the raw materials into powder, and passing through a 40-60 mesh sieve;
[0016] S2, adding distilled water to the powder obtained in step S1, and standing at 0-4℃ for 10-15h; after boiling for 5-10min, the temperature is lowered to 40-55℃, a neutralizing agent is added to adjust the pH of the solution to neutral, a protease is added, and enzyme hydrolysis is carried out for 2-4h; then, water bath is carried out at 80-100℃ for 20-30min; after the temperature is lowered to room temperature, high-speed centrifugation is carried out to obtain the supernatant, which is obtained.
[0017] Preferably, the raw material for preparing the peptide substance is fish meat and toad oil.
[0018] Preferably, the mass ratio of the fish meat to the toad oil is 1:(1-3); more preferably, 1:2.
[0019] Preferably, the fish meat is perch meat.
[0020] Preferably, the solid-liquid ratio of the powder obtained in the step S1 to distilled water is 1g:10-20mL; more preferably, 1g:15mL.
[0021] Preferably, the protease includes one or more of neutral protease, alkaline protease, papain, trypsin, and flavor protease; more preferably, neutral protease and flavor protease.
[0022] Preferably, the mass ratio of the neutral protease to the flavor protease is (4-8):1; more preferably, 7:1.
[0023] Preferably, the enzyme activity of the neutral protease and the flavor protease is 40000-80000U / g; more preferably, 50000U / g.
[0024] The applicant found that the peptide material prepared by using sea bass meat and toad oil with a mass ratio of 1:(1-3) as raw materials can improve the immunity of pets and promote their growth and development. This may be because both of them are rich in protein, amino acids, polyunsaturated fatty acids, vitamins and trace elements such as minerals, which not only promote the normal operation of the immune system, but also provide raw materials for the formation of immune substances in pets. However, for young pets or pets in old age, weakness, or early recovery from illness, their absorption of protein is limited and the absorption efficiency is low, and a large amount of body energy is consumed for absorption. For non-adolescent healthy pets, this only aggravates their physical burden. The applicant unexpectedly found that the peptide material prepared by using neutral protease and flavor protease with a mass ratio of (4-8):1 to enzymatically hydrolyze sea bass and toad oil under specific conditions can be applied to pet cans to improve the immunity of pets, reduce the burden on the intestinal tract of pets, and also improve the appetite of pets. This may be because under specific conditions, neutral protease first decomposes the protein in sea bass meat and toad oil into peptide material, and then flavor protease further degrades the peptide material into amino acids, significantly improves the degree of hydrolysis, increases the generation rate of amino nitrogen, and through the Maillard reaction, the bitter peptide is completely degraded into amino acids and small molecule peptide material, removing the bitterness and improving the flavor of the product, and improving the appetite of pets. The peptide material prepared by decomposing sea bass meat and toad oil as raw materials can be directly absorbed by pets, thereby improving the immunity and appetite of pets, and the decomposition of protein into small molecule fragments can reduce the probability of pet allergic reactions, thereby improving the universality of the prepared pet cans.
[0025] In some preferred embodiments, the neutral protease and the flavor protease are both produced by Chongqing Tianrun Bioproducts Co., Ltd.
[0026] Preferably, the neutralizing agent is one or more of sodium carbonate, sodium bicarbonate, and sodium hydroxide; and more preferably, the neutralizing agent is sodium hydroxide.
[0027] Preferably, the probiotics are Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum, and Lactobacillus plantarum.
[0028] Preferably, the number of viable bacteria in the Lactobacillus acidophilus is ≥1×10 10 / g.
[0029] Preferably, the number of viable bacteria in the Bacillus subtilis is ≥1×10 10 / g.
[0030] Preferably, the number of viable bacteria in the Bifidobacterium longum is ≥10×10 10 / g.
[0031] Preferably, the number of viable bacteria in the Lactobacillus plantarum is ≥1×1010 / g.
[0032] In some preferred embodiments, the Lactobacillus acidophilus, Bifidobacterium longum and Lactobacillus plantarum are purchased from Shaanxi Chenming Biotechnology Co., Ltd., and the Bacillus subtilis is purchased from Hebei Rongheng Fertilizer Co., Ltd.
[0033] Preferably, the mass ratio of the Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum and Lactobacillus plantarum is (1-2):(1-2):(0.5-1.5):1; further preferably, 1.5:1.5:1:1.
[0034] In some preferred embodiments, by selecting the Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum and Lactobacillus plantarum with a mass ratio of (1-2):(1-2):(0.5-1.5):1 as probiotics, not only the immunity of pets can be improved, but also the health of the gastrointestinal tract can be regulated. This may be because, on the one hand, Bifidobacterium longum and Bacillus subtilis can synergistically stimulate immune cells and the immune system of the intestinal mucosa, thereby improving the immune capacity of pets and the intestinal mucosa; on the other hand, Lactobacillus acidophilus and Lactobacillus plantarum can jointly regulate the pH value of the intestinal tract, thereby creating a more favorable growth environment for Bifidobacterium longum and Bacillus subtilis, further promoting their immune-enhancing effect, and at the same time, regulating the balance of intestinal flora and promoting the absorption of nutrients. The addition of too much probiotics is avoided, which may otherwise destroy the ecological balance in pets.
[0035] Preferably, the preparation method of the traditional Chinese medicine extract comprises the following steps: taking traditional Chinese medicine powder, dissolving in 80% ethanol solution, ultrasonicating at 300-400 W for 40-60 min, filtering, and concentrating the filtrate by rotary evaporation to obtain the traditional Chinese medicine extract.
[0036] Preferably, the solid-liquid ratio of the traditional Chinese medicine powder and the ethanol solution is 1 g:20-40 mL.
[0037] Preferably, the traditional Chinese medicine powder is Astragalus, Lentinus edodes and hawthorn.
[0038] Preferably, the mass ratio of the Astragalus, Lentinus edodes and hawthorn is (1-3):(0.5-2):1; further preferably, 2:1:1.
[0039] In some preferred schemes, the astragalus, lentinus edodes and hawthorn are selected in a mass ratio of (1-3):(0.5-2):1, and the effective substances thereof are extracted and applied to the pet cans, so that the immunity of pets can be further improved. This may be because the polysaccharides, vitamin E and trace elements and other nutrients extracted from the three raw materials jointly act, on the one hand, can not only increase the activity of immune cells, but also can improve the antioxidant capacity and reduce the inflammatory response, on the other hand, can promote the proliferation and activation of immune cells by inhibiting the expression of certain cytokines, so as to enhance the immunity of the body.
[0040] Preferably, the mineral component comprises one or more of eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite; and more preferably, the mineral component comprises eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite.
[0041] Preferably, the mass ratio of the eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite is (3-5):(3-5):(1.5-2.5):(1.5-2.5):(1.5-2.5):1; and more preferably, the mass ratio of the eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite is 4:4:2:2:2:1.
[0042] Preferably, the calcium content in the eggshell powder is greater than or equal to 99%.
[0043] The compound vitamin comprises, by weight, 0.01-0.03 parts of vitamin A, 0.05-0.15 parts of vitamin D, 0.02-0.08 parts of vitamin C, 0.08-0.12 parts of vitamin E, 0.05-0.12 parts of pantothenic acid, 0.15-0.3 parts of folic acid and 0.05-0.15 parts of nicotinic acid.
[0044] The second aspect of the present application provides a preparation method of the full-price pet can for improving the immunity of pets, comprising the following steps:
[0045] (1) cutting the meat into a meat paste;
[0046] (2) mixing the supplementary additives and water uniformly to obtain a substance one;
[0047] (3) mixing the meat paste and the substance one uniformly, vacuum sealing after cooking and curing, and sterilizing at 100-120 DEG C for 3-4 hours to obtain the full-price pet can.
[0048] Compared with the prior art, the present application has the following advantages and beneficial effects:
[0049] 1. The application provides a full-ration pet can which can improve the immunity of pets, regulate the intestinal absorption capacity of pets, enhance the physical quality of pets, supplement various nutrients for pets, reduce the risk of allergy, and enable pets, especially weaned young pets, to grow healthily and safely.
[0050] 2. The application adds L-cysteine, L-arginine, taurine and lysine to the can in a mass ratio of (0.5-1):(0.8-1.2):(1-1.5):1, which can improve the immunity and digestive capacity of pets, especially young pets.
[0051] 3. The application uses sea bass meat and toad oil as raw materials of peptide substances in a mass ratio of 1:(1-3), which can improve the immunity of pets and promote the growth and development of pets.
[0052] 4. The application uses neutral protease and flavor protease in a mass ratio of (4-8):1 to enzymatically hydrolyze sea bass and toad oil under specific conditions, and the prepared peptide substances are applied to pet cans, which can improve the immunity of pets, reduce the intestinal burden of pets, and also improve the appetite of pets.
[0053] 5. The application uses Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum and Lactobacillus plantarum as probiotics in a mass ratio of (1-2):(1-2):(0.5-1.5):1, which can not only improve the immunity of pets, but also regulate the health of the gastrointestinal tract.
[0054] 6. The application uses Radix Astragali, Lentinus edodes and hawthorn as traditional Chinese medicines in a mass ratio of (1-3):(0.5-2):1, and extracts effective substances thereof for application in pet cans, which can further improve the immunity of pets. DETAILED DESCRIPTION
[0055] The technical solutions in the embodiments of the application will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.
[0056] The information of raw materials used in the application is as follows:
[0057] The neutral protease and flavor protease have an enzyme activity of 50000 U / g and are purchased from Chongqing Tianlun Biological Products Co., Ltd.
[0058] The effective viable count of Lactobacillus acidophilus is greater than or equal to 1x10 10 / g, and the effective viable count of Bacillus subtilis is greater than or equal to 1x1010 / g, the effective viable cell count of Bifidobacterium longum ≥ 10 × 10 10 / g, the effective viable cell count of Lactobacillus plantarum ≥ 1 × 10 10 / g; Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus plantarum were purchased from Shaanxi Chenming Biological Technology Co., Ltd., and Bacillus subtilis was purchased from Hebei Rongheng Fertilizer Co., Ltd.
[0059] The calcium content in eggshell powder is ≥ 99%, which is purchased from Jiangsu Chuangsheng Biological Engineering Co., Ltd.
[0060] Meat, perch meat, toad oil, milkvetch, shiitake mushroom, hawthorn are all commercially available products, and no specific restrictions are made.
[0061] The remaining raw materials are substances with CAS numbers, and no specific restrictions are made.
[0062] Example 1
[0063] The present embodiment provides a full-price canned pet for improving the immunity of pets, and the preparation raw materials thereof are as follows in terms of percentage by weight: 80% of meat, 3.5% of supplementary additives, and the balance of water.
[0064] The meat is 88% of muscle, 5% of bone-in meat, and 7% of blood-in viscera in terms of percentage by weight.
[0065] The muscle is chicken meat; the bone-in meat is derived from chicken clavicle; and the blood-in viscera is derived from rabbit liver.
[0066] The supplementary additives are as follows in terms of parts by weight: 0.7 parts of amino acids, 1.5 parts of peptide substances, 0.5 parts of probiotics, 1.5 parts of traditional Chinese medicine extracts, 0.2 parts of mineral components, and 1.2 parts of compound vitamins.
[0067] The amino acids are a combination of L-cysteine, L-arginine, taurine, and lysine.
[0068] The mass ratio of L-cysteine, L-arginine, taurine, and lysine is 0.8:1:1.2:1.
[0069] The preparation method of the peptide substances is as follows:
[0070] S1, dry and grind the raw materials into powder, and pass through a 50-mesh sieve;
[0071] S2, add distilled water to the powder obtained in step S1, and stand at 1℃ for 12h, then boil for 8min, and reduce the temperature to 50℃, add a neutralizing agent to adjust the pH of the solution to neutral, add protease, and enzymatically hydrolyze for 3h, then water bath at 90℃ for 25min, reduce the temperature to room temperature, and then high-speed centrifuge to obtain the supernatant.
[0072] The peptide material preparation raw material is fish meat and toad oil.
[0073] The mass ratio of the fish meat and toad oil is 1:2.
[0074] The fish meat is perch meat.
[0075] The solid-liquid ratio of the powder obtained in step S1 and distilled water is 1g:15mL.
[0076] The protease is neutral protease and flavor protease.
[0077] The mass ratio of the neutral protease and flavor protease is 7:1.
[0078] The neutralizing agent is sodium hydroxide.
[0079] The probiotics are lactobacillus acidophilus, bacillus subtilis, bifidobacterium longum, lactobacillus plantarum.
[0080] The mass ratio of the lactobacillus acidophilus, bacillus subtilis, bifidobacterium longum, lactobacillus plantarum is 1.5:1.5:1:1.
[0081] The preparation method of the traditional Chinese medicine extract comprises the following steps: taking traditional Chinese medicinal material powder, dissolving in 80% ethanol solution, ultrasonic treatment at 350W for 50min, filtering, and concentrating the filtrate by rotary evaporation to obtain the traditional Chinese medicine extract.
[0082] The solid-liquid ratio of the traditional Chinese medicinal material powder and the ethanol solution is 1g:30mL.
[0083] The traditional Chinese medicinal material powder is radix astragali, lentinus edodes and hawthorn.
[0084] The mass ratio of the radix astragali, lentinus edodes and hawthorn is 2:1:1.
[0085] The mineral component is eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite.
[0086] The mass ratio of the eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite is 4:4:2:2:2:1.
[0087] In terms of weight parts, the complex vitamin is vitamin A 0.02 parts, vitamin D 0.1 part, vitamin C 0.05 part, vitamin E 0.1 part, pantothenic acid 0.08 part, folic acid 0.2 part and nicotinic acid 0.1 part.
[0088] The preparation method of the full-price pet can for improving pet immunity comprises the following steps:
[0089] (1) cutting the meat into meat paste;
[0090] (2) Mix the supplementary additives and water evenly to obtain substance one;
[0091] (3) Mix the meat paste and substance one evenly, and then vacuum seal the tank after cooking and curing. After sterilization treatment at 110℃ for 3.5h, it is obtained.
[0092] Example 2
[0093] The difference between this example and Example 1 is that the supplementary additives are 0.7 parts of amino acids, 2 parts of peptide substances, 0.8 parts of probiotics, 1.2 parts of traditional Chinese medicine extracts, 0.2 parts of mineral components, and 1.2 parts of compound vitamins by weight.
[0094] Example 3
[0095] The difference between this example and Example 1 is that the mass ratio of neutral protease to flavor protease is 4:1.
[0096] Comparative Example 1
[0097] The difference between this comparative example and Example 1 is that the mass ratio of L-arginine, taurine, and lysine is 1:1.2:1.
[0098] Comparative Example 2
[0099] The difference between this comparative example and Example 1 is that the mass ratio of neutral protease to flavor protease is 1:1.
[0100] Comparative Example 3
[0101] The difference between this comparative example and Example 1 is that the mass ratio of Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum, and Lactobacillus plantarum is 1.5:0.5:1:1.
[0102] Comparative Example 4
[0103] The difference between this comparative example and Example 1 is that the traditional Chinese medicine powder is Astragalus and shiitake mushroom, and the mass ratio of Astragalus to shiitake mushroom is 2:1.
[0104] Performance test
[0105] Select 24 healthy three-month-old weaned kittens each, and randomly divide them into 8 groups, 3 each. Among them, the control group does not add supplementary additives, and the components are consistent with Example 1. The feeding environment is 25℃ and the relative humidity is 55%. Feed 1 meal per day for 1 week, and the feeding amount is 6% of the body weight of the test cat. After 1 week, take blood samples from each test animal for blood routine examination, record the IgG, IgA, and IgM in the serum; At the same time, record the body weight of each animal before and after 1 week, and calculate the total weight gain. All data are averaged. The results are shown in Table 1.
[0106] Table 1 Test results
[0107] IgG / (mg / mL) IgA / (mg / mL) IgM / (mg / mL) Weight gain / kg Example 1 24.83 2.14 1.45 0.65 Example 2 24.27 2.03 1.34 0.63 Example 3 23.93 2.01 1.32 0.62 Comparative Example 1 21.65 1.84 1.21 0.48 Comparative Example 2 19.28 1.77 1.18 0.44 Comparative Example 3 21.98 1.94 1.26 0.53 Comparative Example 4 21.65 1.90 1.23 0.50 Control group 14.89 1.58 0.82 0.35
[0108] The full-price pet cans prepared in Examples 1-3 of the present application can not only improve the immunity of pet kittens, but also supplement various nutritional ingredients for pets, regulate the health of the gastrointestinal tract, and enhance the gastrointestinal absorption capacity, so that the young pets can grow healthily. Comparative Example 1 does not add L-cysteine, Comparative Example 2 has a relatively small amount of neutral protease, Comparative Example 3 has a small amount of Bacillus subtilis, and Comparative Example 4 does not add hawthorn. Although the full-price pet cans prepared have improved immunity and body weight compared with the control group, the improvement is limited. Therefore, the full-price pet cans prepared by using the raw materials and method described in the present application can improve the immunity of pets, enhance the physical fitness of pets, and supplement various nutritional ingredients for pets, so that pets with low immunity can also grow healthily and safely.
[0109] The above is a preferred embodiment of the present application. It should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application. These improvements and refinements should also be considered within the scope of protection of the present application.
Claims
1. A complete canned pet food for improving immunity of pets, characterized in that, The preparation raw materials include, by weight percentage, 70-85% of meat, 1-5% of supplementary additives, and the balance of moisture to 100%; the supplementary additives include, by weight, 0.5-1 parts of amino acids, 1-2 parts of peptide substances, 0.2-0.8 parts of probiotics, 1.2-1.8 parts of traditional Chinese medicine extracts, 0.1-0.5 parts of mineral components, and 1-1.5 parts of compound vitamins; The amino acids are a combination of L-cysteine, L-arginine, taurine and lysine, and the mass ratio of the L-cysteine, L-arginine, taurine and lysine is (0.5-1):(0.8-1.2):(1-1.5):1; The preparation method of the peptide substances includes the following steps: S1, drying and grinding the raw materials into powder, and passing through a 40-60 mesh sieve; S2, adding distilled water to the powder obtained in step S1, and standing at 0-4℃ for 10-15h, boiling for 5-10min, and then cooling to 40-55℃, adding a neutralizing agent to adjust the pH of the solution to neutral, adding a protease, and enzymatically hydrolyzing for 2-4h, and then water-bathing at 80-100℃ for 20-30min, and then cooling to room temperature, and then high-speed centrifuging to obtain the supernatant, which is the peptide substances; The preparation raw materials of the peptide substances are fish meat and toad oil, and the mass ratio of the fish meat to the toad oil is 1:(1-3); the protease is a combination of neutral protease and flavor protease, and the mass ratio of the neutral protease to the flavor protease is (4-7):1; the enzyme activity of the neutral protease and the flavor protease is 40000-80000U / g; The probiotics are a combination of Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum and Lactobacillus plantarum, and the mass ratio of the Lactobacillus acidophilus, Bacillus subtilis, Bifidobacterium longum and Lactobacillus plantarum is (1-2):(1-2):(0.5-1.5):1; The preparation method of the traditional Chinese medicine extracts includes the following steps: taking traditional Chinese medicinal material powder, dissolving in 80% ethanol solution, ultrasonicating at 300-400W for 40-60min, filtering, and then rotary evaporating the filtrate to concentrate, which are the traditional Chinese medicine extracts; the solid-liquid ratio of the traditional Chinese medicinal material powder to the ethanol solution is 1g:20-40mL; the traditional Chinese medicinal material powder is a combination of Radix Astragali, Lentinus edodes and Crataegus pinnatifida Bunge, and the mass ratio of the Radix Astragali, Lentinus edodes and Crataegus pinnatifida Bunge is (1-3):(0.5-2):1; The mineral components are a combination of eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite, and the mass ratio of the eggshell powder, ferrous sulfate, zinc sulfate, potassium iodide, magnesium sulfate and sodium selenite is (3-5):(3-5):(1.5-2.5):(1.5-2.5):(1.5-2.5):
1.
2. The immunity-boosting complete pet canned pet food according to claim 1, characterized by, The meat includes, by weight percentage, 82-90% of muscle, 3-10% of bone-in meat, and 5-15% of blood-in viscera.
3. The immunity-boosting complete pet canned pet food according to claim 1, characterized by, 4. The immunity-boosting complete pet canned pet food according to claim 1, wherein, The complex vitamin includes vitamin A 0.01-0.03 parts, vitamin D 0.05-0.15 parts, vitamin C 0.02-0.08 parts, vitamin E 0.08-0.12 parts, pantothenic acid 0.05-0.12 parts, folic acid 0.15-0.3 parts, and nicotinic acid 0.05-0.15 parts by weight.
5. A method of preparing the complete pet can for improving immunity of a pet according to any one of claims 1 to 4, characterized in that, The method comprises the following steps: (1) cutting the meat into a paste; (2) mixing the supplementary additives and water uniformly to obtain a substance one; (3) mixing the meat paste and the substance one uniformly, vacuum sealing the mixture after cooking and curing, and sterilizing the mixture at 100-120℃ for 3-4h to obtain the product.
Citation Information
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