A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles

Abstract

The invention discloses a method for preparing a metal polyphenol-coated mesoporous silicon-selenium nanoparticle, comprising the steps of: placing a mesoporous silicon-selenium nanoparticle solution in a centrifuge tube, adding a 24mM EGCG aqueous solution, vortexing for 5-20s, adding a 96mM copper chloride aqueous solution, vortexing for 5-20s, ultrasonic treatment after the vortexing ends, centrifugal separation and precipitation after the ultrasonication ends, and washing the precipitation with ultrapure water to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles. The present invention discloses a method for preparing a metal polyphenol-coated mesoporous silicon-selenium nanoparticle, by constructing a Cu-EGCG coordination compound to meet the sustained release of copper and reduce its toxicity to an organism, using the Cu-EGCG coordination compound as a metal polyphenol coating, which can enhance SOD enzyme activity, remove ROS, and exert a good anti-inflammatory effect.

Description

Technical Field

[0001] The invention relates to the technical field of nano-medicine, and in particular to a method for preparing mesoporous silicon-selenium nanoparticles coated with metal polyphenols. Background Art

[0002] Selenium is an essential nutrient for the human body. It is present in the active sites of 25 essential selenoproteins for humans, so it can effectively improve the activity of glutathione peroxidase (GPX), a selenoenzyme related to the immune system. Selenium can enhance the immune response of the body's immune system and has strong antioxidant, immunomodulatory and anti-inflammatory properties.

[0003] Studies have shown that compared with selenium in other valence states, zero-valence selenium has the advantages of good biocompatibility and high bioavailability, and has anti-inflammatory, antioxidant and anti-cancer effects. In addition, selenium nanoparticles (SeNPs) show lower acute toxicity and greater biological activity due to their nanosize effect. However, they tend to aggregate into large clusters in aqueous solutions, resulting in reduced biological activity and bioavailability. Therefore, a new method for constructing selenium nanoparticles with good dispersibility is needed.

[0004] Mesoporous silica nanospheres (MSNs) have been widely used in drug delivery due to their unique physical and chemical properties, including special porous structure, adjustable particle size and pore volume, high specific surface area and good biocompatibility. In addition, depositing host molecules into porous materials can significantly accelerate their reaction rate with guest molecules. Therefore, in order to ensure that the nanoparticles have good dispersibility and higher biological activity, selenium was deposited on the surface of mesoporous silica nanospheres to obtain mesoporous silica nanosphere-selenium nanocomposites.

[0005] Epigallocatechin gallate (EGCG) is the most effective active ingredient in tea polyphenols, with antibacterial, antioxidant, anti-inflammatory and anti-tumor effects. However, the instability of EGCG in water or in vitro physiological fluids limits its further application. Therefore, it is crucial to adopt effective strategies to improve the stability of EGCG for the utilization of EGCG and exert its biological activity, such as introducing biocompatible and biodegradable carriers.

[0006] It is noteworthy that metal polyphenol networks (MPNs) formed by the coordination interactions between polyphenols and metal ions can serve as a versatile and efficient surface modification platform to improve the stability and bioavailability of EGCG. 2+ , Mn 2+ , Cu 2+Metal ions such as copper and iodine can combine with polyphenols to form nanoparticles with therapeutic functions. Among them, some copper coordination compounds also show anti-inflammatory and antioxidant effects. 2+ It is a cofactor of superoxide dismutase (SOD) and can effectively enhance the activity of SOD. Summary of the invention

[0007] The present invention provides a preparation method of metal polyphenol-coated mesoporous silicon-selenium nanoparticles, which overcomes the disadvantage that selenium nanoparticles are easy to agglomerate. Cu-EGCG is used as the metal polyphenol coating, which can not only enhance the activity of SOD enzyme and remove ROS, but also exert a good anti-inflammatory effect; the mesoporous silicon-selenium nanoparticles and Cu-EGCG work synergistically, thereby exerting more therapeutic functions.

[0008] To achieve the above object, the present invention provides a method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles, comprising the following steps:

[0009] S1. Prepare 5-20 mg / mL mesoporous silica solution, add ultrapure water and 20 mM sodium selenite according to the volume ratio of mesoporous silica solution: ultrapure water: sodium selenite = 1:3:1, and stir overnight at room temperature to obtain solution A;

[0010] S2, adding 20-80 mM ascorbic acid to solution A, stirring the reaction, centrifuging the solution, washing the precipitate with ultrapure water, and dispersing it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle solution;

[0011] S3. Place the mesoporous silicon-selenium nanoparticle solution in a centrifuge tube, add 24 mM EGCG aqueous solution, vortex for 5-20 seconds, add 96 mM copper chloride aqueous solution, vortex for 5-20 seconds, ultrasonicate after vortexing, centrifuge and precipitate after ultrasonication, wash the precipitate with ultrapure water to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles.

[0012] Preferably, in step S2, the volume ratio of ascorbic acid:solution A is 1:5.

[0013] Preferably, in step S2, the stirring reaction refers to stirring the reaction at 600 rpm at room temperature for 1 to 4 hours.

[0014] Preferably, in step S2, the centrifugal speed is 10000-13000 rpm, and the centrifugal time is 5 min.

[0015] Preferably, in step S3, the volume ratio of mesoporous silicon-selenium nanoparticle solution: EGCG aqueous solution: copper chloride aqueous solution = 3: (1-3): (1-3).

[0016] Preferably, in step S3, the ultrasonic treatment is performed for 5 min.

[0017] Preferably, in step S3, the centrifugal speed is 10000-13000 rpm, and the centrifugal time is 5 min.

[0018] The present invention provides a method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles, which has the following beneficial effects:

[0019] (1) Se is deposited on the surface of MSNs to obtain MSN-Se nanocomposites, which can prevent nano-Se from aggregating and make them have good dispersibility and higher biological activity;

[0020] (2) A Cu-EGCG coordination compound was constructed. Cu-EGCG can improve the stability of EGCG, meet the sustained release of Cu and reduce the toxicity of Cu to organisms;

[0021] (3) The Cu-EGCG coordination compound was used as a metal polyphenol coating loaded on the surface of mesoporous silica-selenium nanoparticles, which not only sealed the pores of mesoporous silica and improved the stability, but also efficiently delivered Cu-EGCG;

[0022] (4) Mesoporous silica-selenium nanoparticles work synergistically with Cu-EGCG to exert more therapeutic functions.

[0023] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 are transmission electron microscope images of mesoporous silica nanoparticles, mesoporous silicon-selenium nanoparticles, and metal polyphenol-coated mesoporous silicon-selenium nanoparticles, wherein A is a transmission electron microscope image of mesoporous silica nanoparticles, B is a transmission electron microscope image of mesoporous silicon-selenium nanoparticles prepared in Example 1, C is a transmission electron microscope image of mesoporous silicon-selenium nanoparticles coated with metal polyphenols at 100 nm, and D is a transmission electron microscope image of mesoporous silicon-selenium nanoparticles coated with metal polyphenols at 20 nm;

[0025] Figure 2 A is a particle size diagram of metal polyphenol-coated mesoporous silicon-selenium nanoparticles, and B is a potential diagram of mesoporous silicon-selenium nanoparticles (MSeNPs), metal polyphenol Cu-EGCG (CENPs) and metal polyphenol-coated mesoporous silicon-selenium nanoparticles (MSe@CENPs);

[0026] Figure 3 The mapping diagram of mesoporous silicon-selenium nanoparticles coated with metal polyphenols; A is the bright field, B is the distribution of Si elements, C is the distribution of Se elements, and D is the distribution of Cu elements;

[0027] Figure 4 Figure 2 shows the cytotoxicity of metal polyphenol-coated mesoporous silica-selenium nanoparticles.

[0028] Figure 5 A is a bar graph showing the ABTS free radical scavenging rate of mesoporous silicon-selenium nanoparticles coated with metal polyphenols at different concentrations; B is a bar graph showing the DPPH free radical scavenging rate of mesoporous silicon-selenium nanoparticles coated with metal polyphenols at different concentrations;

[0029] Figure 6 A is the superoxide anion O of mesoporous silica-selenium nanoparticles coated with metal polyphenols at different concentrations. 2- Clearance rate bar graph, B is the reduced glutathione clearance rate bar graph of mesoporous silicon-selenium nanoparticles coated with metal polyphenols at different concentrations;

[0030] Figure 7 These are experimental diagrams of mesoporous silica-selenium nanoparticles (MSeNPs), metal polyphenol Cu-EGCG (CE NPs), and metal polyphenol-coated mesoporous silica-selenium nanoparticles (MSe@CENPs) clearing reactive oxygen species in macrophages, where A is the flow cytometry result diagram and B is the fluorescence quantification diagram;

[0031] Figure 8 These are the experimental results of regulating macrophage polarization by metal polyphenol-coated mesoporous silicon-selenium nanoparticles, where A is the number of M1 macrophages and B is the number of M2 macrophages. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages disclosed in the embodiments of the present invention clearer, the embodiments of the present invention are further described in detail in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the embodiments of the present invention and are not intended to limit the embodiments of the present invention. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application. Examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions.

[0033] It should be noted that the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or server that includes a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units that are not explicitly listed or inherent to these processes, methods, products or devices.

[0034] Like reference numerals and letters denote similar items in the following drawings, and thus, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0035] Embodiment 1

[0036] A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles comprises the following steps:

[0037] S1. Take 1 mL of 5 mg / mL mesoporous silica solution, add 3 mL of ultrapure water, add 1 mL of 20 mM sodium selenite, and stir at room temperature overnight to obtain solution A.

[0038] S2. Add 1 mL of 20 mM ascorbic acid to solution A, stir at 600 rpm for 1 hour at room temperature, centrifuge the mixed aqueous solution at 10,000 rpm for 4 minutes after stirring the reaction, wash the precipitate with ultrapure water 3 times and disperse it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle solution.

[0039] S3. Take 1.5 mL of the mesoporous silicon-selenium nanoparticle solution and place it in a centrifuge tube, add 0.5 mL of a 24 mM EGCG aqueous solution, and vortex for 5 seconds; then add 0.5 mL of a 96 mM copper chloride aqueous solution, and vortex for 5 seconds. After vortexing, perform ultrasonic treatment for 5 minutes.

[0040] S4. After the ultrasound is finished, the solution is centrifuged at 10000 rpm for 5 min, and the precipitate is washed with ultrapure water three times to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles.

[0041] Embodiment 2

[0042] A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles comprises the following steps:

[0043] S1. Take 1 mL of 10 mg / mL mesoporous silica solution, add 3 mL of ultrapure water, add 1 mL of 20 mM sodium selenite, and stir at room temperature overnight to obtain solution A.

[0044] S2. Add 1 mL of 40 mM ascorbic acid to solution A, stir at 600 rpm for 2 hours at room temperature, centrifuge the mixed aqueous solution at 11000 rpm for 5 minutes after stirring the reaction, wash the precipitate with ultrapure water 3 times and disperse it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle (MSe NPs) solution.

[0045] S3. Take 1.5 mL of the mesoporous silicon-selenium nanoparticle solution and place it in a centrifuge tube, add 0.75 mL of a 24 mM EGCG aqueous solution, and vortex for 10 seconds; then add 0.75 mL of a 96 mM copper chloride aqueous solution, and vortex for 10 seconds. After vortexing, perform ultrasonic treatment for 5 minutes.

[0046] S4. After the ultrasound, the solution was centrifuged at 11000 rpm for 5 min, and the precipitate was washed with ultrapure water three times to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles (MSe@CE NPs).

[0047] Embodiment 3

[0048] A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles comprises the following steps:

[0049] S1. Take 1 mL of 15 mg / mL mesoporous silica solution, add 3 mL of ultrapure water, add 1 mL of 20 mM sodium selenite, and stir at room temperature overnight to obtain solution A.

[0050] S2. Add 1 mL of 60 mM ascorbic acid to solution A, stir at 600 rpm for 3 hours at room temperature, centrifuge the mixed aqueous solution at 12000 rpm for 5 minutes after the stirring reaction, wash the precipitate with ultrapure water 3 times and disperse it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle solution.

[0051] S3. Take 1.5 mL of the mesoporous silicon-selenium nanoparticle solution and place it in a centrifuge tube, add 1 mL of a 24 mM EGCG aqueous solution, and vortex for 20 seconds; then add 1 mL of a 96 mM copper chloride aqueous solution, and vortex for 20 seconds. After vortexing, perform ultrasonic treatment for 5 minutes.

[0052] S4. After the ultrasound is finished, the solution is centrifuged at 12000 rpm for 5 min, and the precipitate is washed with ultrapure water three times to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles.

[0053] Embodiment 4

[0054] A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles comprises the following steps:

[0055] S1. Take 1 mL of 20 mg / mL mesoporous silica solution, add 3 mL of ultrapure water, add 1 mL of 20 mM sodium selenite, and stir at room temperature overnight to obtain solution A.

[0056] S2. Add 1 mL of 80 mM ascorbic acid to solution A, stir at 600 rpm for 4 hours at room temperature, centrifuge the mixed aqueous solution at 13000 rpm for 5 minutes after stirring the reaction, wash the precipitate with ultrapure water 3 times and disperse it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle solution.

[0057] S3. Take 1.5 mL of the mesoporous silicon-selenium nanoparticle solution and place it in a centrifuge tube, add 1.5 mL of a 24 mM EGCG aqueous solution, and vortex for 20 seconds; then add 1.5 mL of a 96 mM copper chloride aqueous solution, and vortex for 20 seconds. After vortexing, perform ultrasonic treatment for 5 minutes.

[0058] S4. After the ultrasound is finished, the solution is centrifuged at 13000 rpm for 5 min, and the precipitate is washed with ultrapure water three times to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles.

[0059] Embodiment 5

[0060] According to the inductively coupled plasma test of Cu element in MSe@CE NPs, the quantitative analysis results determined that Cu 2+ The amount of copper chloride aqueous solution and EGCG aqueous solution was determined based on the result. A 24 mM EGCG aqueous solution was vortexed for 5 seconds; a 96 mM copper chloride aqueous solution was added and vortexed for 5 seconds. After the vortexing, ultrasonic treatment was performed for 5 minutes and then reacted at room temperature for 24 hours to obtain metal polyphenol CE NPs.

[0061] Experimental Testing

[0062] (1) Figure 1 As shown, A is a transmission electron microscope photo of mesoporous silica nanoparticles, and B and C are transmission electron microscope photos of the metal polyphenol-coated mesoporous silicon-selenium nanoparticles prepared in Example 1 at different magnifications. It can be seen that there is a transparent film on the surface of the metal polyphenol-coated mesoporous silicon-selenium nanoparticles, which indicates that the mesoporous silicon-selenium nanoparticles are successfully coated with the Cu-EGCG coating.

[0063] (2) Figure 2 As shown, A is the particle size diagram of metal polyphenol-coated mesoporous silicon-selenium nanoparticles; it shows that the metal polyphenol-coated mesoporous silicon-selenium nanoparticles are well dispersed in water, and the average hydrodynamic diameter is 180nm.

[0064] B is the potential diagram of mesoporous silicon-selenium nanoparticles (MSe NPs), metal polyphenol Cu-EGCG (CE NPs) and metal polyphenol-coated mesoporous silicon-selenium nanoparticles (MSe@CE NPs).

[0065] After the MSe NPs (22.1 mV) were coated by the CE NPs (−7.83 mV), the zeta potential of the MSe@CE NPs changed to −9.03 mV, indicating that the MSe@CE NPs displayed the potential of the CE NPs, further confirming that the MSe@CE NPs were successfully constructed.

[0066] (3) Figure 3 This is the mapping diagram of metal polyphenol-coated mesoporous silicon-selenium nanoparticles; A is the bright field, B is the distribution of Si element, C is the distribution of Se element, and D is the distribution of Cu element. It shows that Si and Se elements are mainly distributed in the center, while Cu element is distributed in the center and around, which also confirms the successful construction of MSe@CE NPs.

[0067] (4) Cytotoxicity assay

[0068] RAW264.7 cells were seeded into 96-well plates (1×10 4 Cells / well) were cultured in a 37°C, 5% CO2 incubator. After 24 hours, the old culture medium was discarded, and 200 μL of culture medium containing different concentrations (0 mg / L, 0.625 mg / L, 1.25 mg / L, 2.5 mg / L, 5 mg / L, 10 mg / L) of the metal polyphenol-coated mesoporous silicon-selenium nanoparticles prepared in Example 1 was added, and the culture was continued in the incubator. After 24 hours, the old culture medium was discarded, 100 μL of thiazolyl blue MTT solution was added to each well, and after continuing to culture in the incubator for 4 hours, the culture medium in the culture well was sucked off, 100 μL of dimethyl sulfoxide (DMSO) solution was added to each well, and the well was placed on a 37°C shaker and shaken for 10 minutes. The absorbance of each well at 570 nm was measured with an enzyme marker, and the group of mesoporous silicon-selenium nanoparticles coated with 0 mg / L metal polyphenols was taken as the Control group, and the cell survival rate of each group relative to the Control group was calculated.

[0069] The results are as follows Figure 4 As shown in the figure, the concentration of MSe@CeNPs is basically linearly related to its cytotoxicity to RAW264.7 cells. Although the higher the concentration, the greater the toxicity to the cells, when the particle concentration reaches 10 mg / L, the survival rate of RAW264.7 cells still remains at about 87%, indicating that the metal polyphenol-coated mesoporous silicon-selenium nanoparticles of the present invention have good biocompatibility.

[0070] (5) ABTS free radical scavenging: The ABTS free radical scavenging activity of MSe@CENPs was detected using an ABTS free radical scavenging ability detection kit. The MSe@CENPs prepared in Example 1 were diluted into solutions of 25, 50, 100, 200, and 400 μg / mL. After sufficient reaction with an equal amount of ABTS free radical solution, the absorbance of the solution at 405 nm was measured using an ELISA instrument. Three replicates were set for each concentration. On this basis, the ABTS free radical scavenging percentages of MSe@CENPs at different concentrations were calculated. The results are shown in Figure 2. Figure 5 As shown in A.

[0071] Scavenging of DPPH free radicals: The DPPH free radical scavenging activity of MSe@CENPs was detected using a DPPH free radical scavenging ability detection kit. The MSe@CENPs prepared in Example 1 were diluted into solutions of 200, 400, 800, 1200, and 3600 μg / mL. After sufficient reaction with an equal amount of DPPH free radical solution, the absorbance of the solution at 515 nm was measured using an ELISA instrument. Three replicates were set for each concentration. On this basis, the DPPH free radical scavenging percentages of MSe@CENPs at different concentrations were calculated. The results are shown in Figure 2. Figure 5 As shown in B.

[0072] Depend on Figure 5 It can be seen that MSe@CENPs can effectively scavenge ABTS and DPPH in vitro, and it has a dose-dependent free radical scavenging ability.

[0073] (6)O 2- Scavenging (SOD enzyme activity): The superoxide dismutase (SOD) activity detection kit was used to detect the SOD enzyme activity in MSe@CENPs. 2- , O 2- It can reduce nitroblue tetrazolium to generate blue formazan, which absorbs at 560nm; SOD enzyme can remove O 2- , thereby inhibiting the formation of formazan, and the ultraviolet absorption at 560nm decreased accordingly.

[0074] The MSe@CENPs in Example 1 were diluted into solutions of different concentrations of 100, 200, 400, 800, and 1600 μg / mL, and another group was set as a blank control group. Three replicates were set for each concentration. After the reaction was completed, the absorbance of the solution at 560 nm was measured using an ELISA instrument. On this basis, the O 2- The scavenging ability reflects the SOD enzyme activity of the particles. Figure 6 As shown in A.

[0075] Elimination of reduced glutathione (GPX enzyme activity): The glutathione peroxidase (GSH-Px / GPX) activity detection kit was used to measure the GPX enzyme activity in MSe@CENPs. GPX enzyme can catalyze reduced glutathione (GSH) to generate oxidized glutathione (GSSG), reducing toxic hydrogen peroxide to non-toxic hydroxyl compounds (DTNB), which have a characteristic absorption peak at 412nm. The decrease in absorbance at 412nm can reflect the activity of GPX.

[0076] The MSe@CENPs in Example 1 were diluted into solutions of different concentrations of 25, 50, 75, 100, 200, and 400 μg / mL, and another group was set as a blank control group. Three replicates were set for each concentration. After the reaction was completed, the absorbance of the solution at 412 nm was measured using an ELISA instrument. On this basis, the GSH scavenging capacity of MSe@CENPs of different concentrations was calculated to reflect the GPX enzyme activity of the particles. The results are shown in Figure 6 As shown in B.

[0077] The results showed that MSe@CENPs could effectively scavenge superoxide anions O in vitro. 2-, reduced glutathione, and it is dose-dependent, which also shows that the particles have SOD and GPX enzyme activities.

[0078] (7) Intracellular scavenging of reactive oxygen species: RAW264.7 cells were seeded into 24-well plates (1.5×10 5 Cells / well) were cultured overnight. Stimulated with LPS for 24 hours, the original culture medium was removed, and then the complete culture medium containing MSe NPs (Example 1), CE NPs (Example 5) and MSe@CE NPs (Example 1) was incubated with the cells for 24 hours. The cells were collected directly from the well plate into a flow tube with a scraper, stained with DCFH-DA at 37°C for 20 minutes, washed 3 times with PBS, and the original culture medium was discarded. The cells were stained with DCFH-DA at 37°C for 20 minutes, washed 3 times with PBS, and the fluorescence intensity of DCFH-DA was detected by flow cytometry.

[0079] The results are as follows Figure 7 As shown, A is the flow cytometry result diagram, and B is the fluorescence quantification diagram.

[0080] The results showed that the fluorescence intensity of reactive oxygen species was the strongest in the LPS group, while the fluorescence intensity of reactive oxygen species in cells of each experimental group with the addition of nanoparticles showed a decrease to varying degrees. It can be found that the effect of MSe@CE NPs in eliminating reactive oxygen species is better than that of MSe NPs and CE NPs.

[0081] (8) Regulation of macrophage polarization: RAW264.7 cells were implanted into 24-well plates (1.5×10 5 Cells / well) were cultured overnight. Stimulated with LPS for 24 h, the original culture medium was removed, and then the complete culture medium containing MSe NPs (Example 1), CE NPs (Example 5) and MSe@CE NPs (Example 1) was incubated with the cells for 24 h. The cells were collected directly from the well plate into a flow tube with a scraper, and stained with flow antibodies APC F4 / 80, FITC CD86, and PE CD206 at 4°C in the dark for 1 h. After washing with PBS three times, the cells were detected by flow cytometry.

[0082] The results are as follows Figure 8 As shown in the figure, after adding LPS, macrophages differentiated into M1 and M2 macrophages. After treatment with MSe@CENPs, the number of M1 pro-inflammatory macrophages decreased, while the number of M2 anti-inflammatory macrophages increased, indicating that MSe@CE NPs can exert its anti-inflammatory effect by regulating macrophage polarization.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles, characterized in that: The steps include: S1. Prepare 5-20 mg / mL mesoporous silica solution, add ultrapure water and 20 mM sodium selenite according to the volume ratio of mesoporous silica solution: ultrapure water: sodium selenite = 1:3:1, and stir overnight at room temperature to obtain solution A; S2, adding 20-80 mM ascorbic acid to solution A, stirring the reaction, centrifuging the solution, washing the precipitate with ultrapure water, and dispersing it in ultrapure water to obtain a mesoporous silicon-selenium nanoparticle solution; S3. Place the mesoporous silicon-selenium nanoparticle solution in a centrifuge tube, add 24 mM EGCG aqueous solution, vortex for 5-20 seconds, add 96 mM copper chloride aqueous solution, vortex for 5-20 seconds, ultrasonicate after vortexing, centrifuge and precipitate after ultrasonication, wash the precipitate with ultrapure water to obtain metal polyphenol-coated mesoporous silicon-selenium nanoparticles.

2. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S2, the volume ratio of ascorbic acid to solution A is 1:

5.

3. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S2, the stirring reaction refers to stirring the reaction at 600 rpm at room temperature for 1 to 4 hours.

4. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S2, the centrifugal speed is 10000-13000 rpm, and the centrifugal time is 5 min.

5. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S3, the volume ratio of mesoporous silicon-selenium nanoparticle solution: EGCG aqueous solution: copper chloride aqueous solution is 3: (1-3): (1-3).

6. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S3, ultrasonic treatment is performed for 5 min.

7. The method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to claim 1, characterized in that: In step S3, the centrifugal speed is 10000-13000 rpm, and the centrifugal time is 5 min.

8. Metal polyphenol-coated mesoporous silicon-selenium nanoparticles prepared by the method for preparing metal polyphenol-coated mesoporous silicon-selenium nanoparticles according to any one of claims 1 to 7.

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