A black hair composition containing Paris polyphylla extract and preparation method thereof

Abstract

The present invention relates to the technical field of hair preparations, and in particular to a black hair composition containing a Paris polyphylla extract and a preparation method thereof. The raw materials for preparing the composition of the present invention contain Paris polyphylla extract, Polygonum multiflorum extract, Eclipta prostrata extract, Ligustrum lucidum fruit extract, Angelica sinensis extract, Ginseng extract, Clove extract, Selaginella thunbergii extract, Tea tree extract and Cinchona pubescens extract. The black hair composition of the present invention is prepared using plant raw materials, has good safety, and can effectively regulate the expression of tyrosinase in cells to better regulate the production of melanin, and ultimately affect the color of hair.

Description

Technical Field

[0001] The invention relates to the technical field of hair preparations, and in particular to a black hair composition containing a Paris polyphylla extract and a preparation method thereof. Background Art

[0002] Hair is a keratinized appendage on the skin and a special deformable organ of epidermal differentiation. It mainly protects the body and beautifies the appearance. The external environment and internal factors of the body can cause premature graying of hair. Traditional Chinese medicine summarizes the causes of gray hair as deficiency of both heart and kidney, deficiency of liver and kidney, deficiency of both spleen and kidney, and excessive blood heat. Modern medical theory believes that the cause of gray hair is mainly due to the loss of pigment in the hair shaft and the loss or reduction of tyrosinase activity in the hair bulb. Tyrosinase (EC1.14.18.1) is the most critical rate-limiting enzyme in its synthesis process. Studies have shown that tyrosinase has two main functions: one is to hydroxylate monophenols as a monophenolase to produce o-diphenols, and the other is to oxidize o-diphenols as a diphenolase to produce o-quinones. Tyrosinase is only produced by melanocytes, and the amount of melanin synthesis is related to the activity of tyrosinase. The production of melanin can be effectively regulated by regulating the expression of tyrosinase in cells, which ultimately affects the color of skin or hair. Therefore, by properly activating the expression of tyrosinase through appropriate substances and restoring melanin synthesis to normal, it is possible to turn gray hair black.

[0003] Therefore, providing a product that effectively activates tyrosinase and achieves black hair is a technical problem that needs to be solved at present. Summary of the invention

[0004] The present invention provides a black hair composition containing a Paris polyphylla extract and a preparation method thereof. The black hair composition of the present invention is prepared using plant raw materials, has good safety, and can effectively regulate the expression of tyrosinase in cells to better regulate the production of melanin, ultimately affecting the color of hair.

[0005] The raw materials for preparing the composition of the invention include yunnan paridis extract, polygonum multiflorum extract, eclipta extract, glossy privet fruit extract, angelica extract, ginseng extract, clove extract, selaginella extract, smilax china extract and cinchona extract.

[0006] Among them, Yunnan Paris contains a large amount of steroidal saponins, which have multiple physiological activities such as hemostasis, analgesia, anti-inflammatory, anti-tumor, and immunomodulation. It can treat scalp inflammation, promote scalp metabolism, and promote hair follicle regeneration.

[0007] Polygonum multiflorum has the effect of blackening hair and can improve gray hair to a certain extent. It contains rich active ingredients that can nourish hair, promote the formation of melanin, and make hair more black and shiny. Polygonum multiflorum extract has the effect of nourishing the liver and kidneys, and replenishing the essence and blood. It can enhance the liver and kidney functions and promote the circulation of qi and blood in the body, thereby helping hair growth. At the same time, it can also nourish the scalp, improve the scalp environment, and provide healthy growth conditions for hair. Improve hair loss. Polygonum multiflorum extract can improve problems such as dry hair and split ends, making hair smoother and more shiny. It can also repair damaged hair and reduce the breakage and split ends of hair caused by damage from the external environment.

[0008] Eclipta prostrata contains a variety of flavonoids such as quercetin, luteolin, apigenin, isorhamnetin, etc., as well as coumarins such as wedelolic acid, demethylwedelactone and triterpenoid components such as oleanolic acid, ecliptaside, and echinocystic acid. It has multiple functions such as replenishing qi and blood, benefiting the liver and kidneys, and blackening hair. Eclipta prostrata can promote the synthesis of melanin, making hair more black and shiny. Mixing Eclipta prostrata powder with clean water and washing hair can effectively improve hair color and gradually turn white hair black. It is rich in vitamins and minerals, which can provide necessary nutrients for hair and enhance its toughness and luster.

[0009] Fructus Ligustri Lucidi contains a variety of glycosides and phenolic acids such as ligustri glycoside, oleuropein, oleanolic acid, etc. These ingredients have anti-aging, liver protection, neuroprotection and antibacterial activities, and are beneficial to the essence and blood, blackening the hair, strengthening the muscles and bones, etc.; they can also stimulate tyrosinase activity and promote melanin production, and have a therapeutic effect on the transformation of white hair to black. At the same time, they can improve scalp flora, regulate scalp immunity, treat scalp inflammation, and promote hair follicle regeneration.

[0010] Ginseng contains ginseng polysaccharides and ginsenosides, which have the activities of promoting metabolism, enhancing cell vitality, anti-aging, lowering blood lipids and protecting myocardium. It has the effects of replenishing vitality, restoring pulse and strengthening immunity. Ginseng extract is a natural plant ingredient, which is rich in nutrients such as ginsenosides, ginsenotriol, ginseng polysaccharides, methionine, etc., and has a very good nourishing effect. Ginseng extract is rich in ginsenosides, which can stimulate the division of hair follicle cells and promote hair growth. At the same time, ginseng extract can increase blood circulation in the scalp and make the hair absorb more nutrients, which is also very beneficial to the health of hair. Ginseng saponins and ginseng polysaccharides in ginseng extract are good protective agents, which can form a protective film, enhance the toughness of hair, and prevent hair breakage and split ends. Methionine in ginseng extract is a natural moisturizing ingredient that can deeply nourish hair and make hair more supple and shiny. The natural antibacterial ingredients in ginseng extract can remove excess oil from the scalp, regulate the lipid metabolism of the scalp, and avoid excessive oil secretion from the scalp causing greasy hair.

[0011] Cloves contain eugenol, acetyleugenol, caryophyllene, vanillin and esters; they have the effects of regulating qi and blood, anti-inflammatory and bactericidal, can effectively improve hair quality and promote the transformation of white hair to black.

[0012] Selaginella is a common plant for external treatment of hair loss. Its extracts mainly include biflavonoid compounds and various phenolic acids, which can inhibit the excessive secretion of scalp oil. It can effectively activate tyrosinase and promote hair darkening. It also has antibacterial properties, can enhance blood circulation, control dermatitis, stimulate hair growth, and activate blood circulation and regulate blood circulation.

[0013] Angelica contains ligustilide, butenoyl lactone, ferulic acid, niacin, sucrose and some sesquiterpenoid compounds. It has anti-aging, antibacterial, blood-enriching and blood-nourishing effects, and can regulate the immune system, promote blood circulation, and improve skin health.

[0014] Kidney tea, also known as ya nuomiao and cat's whisker grass, contains flavonoids and flavonoid glycosides, phenolic acids, diterpenes, etc., which have the effect of improving the metabolic environment of the scalp and promoting hair regeneration.

[0015] Cinchona contains quinine alkaloids and a variety of flavonoids, which can have antibacterial and anti-inflammatory effects, regulate scalp flora, improve the scalp metabolic environment, promote hair regeneration, protect hair follicle cells, and relieve hair loss.

[0016] The black hair composition containing the Paris polyphylla extract provided by the invention comprises, by weight, 0.5-1 parts of the Paris polyphylla extract, 0.1-0.25 parts of the Polygonum multiflorum extract, 0.1-0.25 parts of the Eclipta prostrata extract, 0.1-0.25 parts of the Ligustrum lucidum fruit extract, 0.05-0.1 parts of the Angelica sinensis extract, 0.1-0.25 parts of the Ginseng extract, 0.05-0.1 parts of the Clove extract, 0.05-0.1 parts of the Selaginella thunbergii extract, 0.05-0.25 parts of the Herba Cynoglossi extract and 0.05-0.1 parts of the Cinchona quinata extract.

[0017] Furthermore, the preparation method of the Paris polyphylla extract comprises: crushing the Paris polyphylla, adding water, heating, ultrasonic-assisted extraction to obtain an extract, filtering, concentrating the filtrate, and drying to obtain the Paris polyphylla extract.

[0018] Furthermore, the ultrasound-assisted extraction time is 20-120 minutes

[0019] Furthermore, the heating is heating to 40-60°C.

[0020] Furthermore, the amount of water added is 8-20 times the mass of the crushed Paris polyphylla.

[0021] Furthermore, the preparation method of the Polygonum multiflorum extract includes: crushing the Polygonum multiflorum, adding an extraction solvent according to a solid-liquid ratio of 1:7-18, heating to 45-62°C, ultrasonic-assisted extraction for 25-80 minutes to obtain an extract, filtering, concentrating the filtrate, and drying to obtain the Polygonum multiflorum extract.

[0022] Furthermore, the extraction solvent is an ethanol aqueous solution with a volume concentration of 10-25%.

[0023] Furthermore, the Eclipta prostrata extract, Ligustrum lucidum fruit extract, Angelica sinensis extract, Panax ginseng extract, Clove extract, Selaginella thunbergii extract, Herba Cynanchum sphaerocephalae extract and Cinchona pubescens extract can all be prepared according to the preparation method of Polygonum multiflorum extract.

[0024] Furthermore, the mass ratio of the Eclipta prostrata extract to the Herba Cynanchifoliae extract is 1:0.8-1.5, 1:0.85-1.45, and 1:0.9-1.4.

[0025] The invention also provides a hair care essence, which contains the composition, ethanol and water.

[0026] Furthermore, the hair care essence comprises, by mass, 0.5-1 parts of Paris polyphylla extract, 0.1-0.25 parts of Polygonum multiflorum extract, 0.1-0.25 parts of Eclipta prostrata extract, 0.1-0.25 parts of Ligustrum lucidum fruit extract, 0.05-0.1 parts of Angelica sinensis extract, 0.1-0.25 parts of Panax ginseng extract, 0.05-0.1 parts of Clove extract, 0.05-0.1 parts of Selaginella tataricus extract, 0.05-0.25 parts of Camellia ternata extract, 0.05-0.1 parts of Cinchona quinata extract, 20-30 parts of anhydrous ethanol and 70-80 parts of water.

[0027] Furthermore, in the hair care essence, the mass ratio of the Eclipta prostrata extract to the Herba Cynanchifoliae extract is 1:0.8-1.5.

[0028] The present invention also provides a method for preparing the composition, comprising the step of mixing various extracts.

[0029] The present invention also provides a method for preparing the hair care essence, comprising the steps of adding water and anhydrous ethanol into a container, starting stirring, then adding various extracts, and continuing stirring and sterilizing.

[0030] The present invention also provides application of the above composition and hair care essence in preparing medicine or cosmetics.

[0031] Furthermore, the medicine includes an external skin medicine.

[0032] The beneficial effects of the present invention are as follows:

[0033] The invention utilizes a reasonable combination of traditional Chinese medicine ingredients, has better safety and has a good activation effect on tyrosinase; the raw materials and dosage of the composition of the invention are reasonably matched, and the problem of graying hair is solved from the root.

[0034] The active ingredients of the black hair composition of the present invention can promote the activation of tyrosinase, effectively promote the production of melanin, and give the effect of turning white hair into black.

[0035] The combination of the Paris polyphylla extract, the Polygonum multiflorum extract, the Eclipta prostrata extract, the Ligustrum lucidum fruit extract, the Angelica sinensis extract, the Panax ginseng extract, the Clove extract, the Selaginella tataricus extract, the Herba Cynoglossi extract and the Cinchona pubescens extract can make the prepared product have a better tyrosinase activation effect.

[0036] The present invention uses a combination of Eclipta prostrata extract and Herba Sinensis extract to effectively activate tyrosinase activity and impart a black-white hair effect. The combination of the two can mutually promote the increase of tyrosinase activity and produce melanin more efficiently. When the mass ratio of the Eclipta prostrata extract to the Herba Sinensis extract is 1:0.8-1.5, the essence promotes higher tyrosinase activity.

[0037] The preparation process of the invention is simple, the raw material source is abundant, the prepared composition and essence milk are green, environmentally friendly, have excellent effects, and have good application prospects. DETAILED DESCRIPTION

[0038] The technical solution of the present invention will be explained below in conjunction with specific implementation methods, and the effect of the technical solution of the present invention will be clearer and more specific.

[0039] 1. Preparation of Extracts

[0040] The preparation method of the Yunnan Paris polyphylla extract includes: crushing the dried Yunnan Paris polyphylla rhizome, adding water at a solid-liquid ratio of 1:10, heating to 50°C, performing ultrasonic-assisted extraction for 30 minutes at an ultrasonic frequency of 60KHz and a power of 200W to obtain the extract, filtering with a 0.5-micron pore size filter membrane, concentrating the filtrate at 62°C under reduced pressure to a filtrate mass of 16.2%, and freeze-drying at -40°C to a water content of 3.78% to obtain the Yunnan Paris polyphylla extract.

[0041] The preparation method of the Polygonum multiflorum extract comprises: crushing the dried Polygonum multiflorum root, adding an ethanol aqueous solution with a volume concentration of 18% according to a solid-liquid ratio of 1:12, heating to 48°C, performing ultrasonic assisted extraction for 35 minutes with an ultrasonic frequency of 60KHz and a power of 200W to obtain the extract, then filtering with a filter membrane with a pore size of 0.5 micron, concentrating the filtrate under reduced pressure at 58°C to a filtrate mass of 15.1%, and freeze-drying at -35°C to a water content of 4.07% to obtain the Polygonum multiflorum extract.

[0042] The preparation method of the Eclipta prostrata extract comprises: crushing dried Eclipta prostrata leaves, adding 24% ethanol aqueous solution with a volume concentration according to a solid-liquid ratio of 1:10, heating to 50°C, performing ultrasonic assisted extraction with an ultrasonic frequency of 60KHz and a power of 200W for 30 minutes to obtain an extract, then filtering with a filter membrane with a pore size of 0.5 micron, concentrating the filtrate under reduced pressure at 56°C to a filtrate mass of 17.6%, and freeze-drying at -37°C to a water content of 4.52%, to obtain the Eclipta prostrata extract.

[0043] The preparation method of the Ligustrum lucidum fruit extract comprises: crushing the dried Ligustrum lucidum fruit, adding a 12% ethanol aqueous solution with a volume concentration according to a solid-liquid ratio of 1:10, heating to 50°C, performing ultrasonic assisted extraction for 30 minutes with an ultrasonic frequency of 60KHz and a power of 200W to obtain an extract, then filtering with a 0.5-micron filter membrane, concentrating the filtrate under reduced pressure at 58°C to a filtrate mass of 17.2%, and freeze-drying at -35°C to a water content of 3.56%, to obtain the Ligustrum lucidum fruit extract.

[0044] The preparation method of angelica extract includes: crushing dried angelica root, adding 17% ethanol aqueous solution with a volume concentration according to a solid-liquid ratio of 1:16, heating to 50°C, ultrasonic frequency of 60KHz and power of 200W for ultrasonic-assisted extraction for 35 minutes to obtain the extract, and then filtering with a 0.5 micron pore size filter membrane, concentrating the filtrate under reduced pressure at 57°C to 12.3% of the filtrate mass, and freeze-drying at -35°C to 3.92% of the water mass to obtain the angelica extract.

[0045] The preparation method of ginseng extract includes: crushing dried ginseng roots, adding 20% ​​ethanol aqueous solution with a volume concentration of 1:15 according to the solid-liquid ratio, heating to 52°C, ultrasonically assisted extraction with an ultrasonic frequency of 60KHz and a power of 200W for 35 minutes to obtain an extract, and then filtering with a 0.5-micron filter membrane, concentrating the filtrate under reduced pressure at 57°C to a filtrate mass of 15.1%, and freeze-drying at -40°C to a water content of 3.17% to obtain the ginseng extract.

[0046] Clove extract: Crush the dried clove flowers, add 12.5% ​​ethanol aqueous solution at a solid-liquid ratio of 1:15, heat to 50°C, and perform ultrasonic assisted extraction at an ultrasonic frequency of 60KHz and a power of 200W for 30 minutes to obtain an extract, which is then filtered through a 0.5-micron filter membrane, and the filtrate is concentrated under reduced pressure at 55°C to a filtrate mass of 10.7%, and freeze-dried at -35°C to a water content of 3.54% to obtain a clove extract.

[0047] Selaginella extract: The dried whole herb of Selaginella was crushed, and an ethanol aqueous solution with a volume concentration of 18.3% was added according to a solid-liquid ratio of 1:10, and the mixture was heated to 50°C, and ultrasonic-assisted extraction was performed at a frequency of 60 kHz and a power of 200 W for 40 minutes to obtain an extract, which was then filtered through a 0.5 μm filter membrane, and the filtrate was concentrated under reduced pressure at 57°C to a filtrate mass of 14.5%, and freeze-dried at -35°C to a water content of 3.22% to obtain a Selaginella extract.

[0048] Kidney tea extract: The dried kidney tea leaves are crushed, and a 15.5% ethanol aqueous solution is added at a solid-liquid ratio of 1:14, heated to 50°C, and ultrasonically assisted extraction is performed at a frequency of 60 kHz and a power of 200 W for 30 minutes to obtain an extract, which is then filtered through a 0.5 micron filter membrane, and the filtrate is concentrated under reduced pressure at 58°C to a filtrate mass of 13.4%, and freeze-dried at -35°C to a water content of 3.76% to obtain the kidney tea extract.

[0049] Cinchona extract: The dried bark of Cinchona is crushed, and a 12.8% ethanol aqueous solution is added at a solid-liquid ratio of 1:10, and the mixture is heated to 50°C, and ultrasonic-assisted extraction is performed at a frequency of 60 kHz and a power of 200 W for 35 minutes to obtain an extract, which is then filtered through a 0.5 μm filter membrane, and the filtrate is concentrated under reduced pressure at 57°C to a filtrate mass of 15.0%, and freeze-dried at -35°C to a water content of 3.55%, to obtain a Cinchona extract.

[0050] 2. Preparation and testing of hair care essence

[0051] The extract prepared above is used to prepare hair care essence.

[0052] Hair Care Essence 1:

[0053] In terms of mass parts, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.2 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.2 parts of Camellia oleifera extract, 0.08 parts of Cinchona quinata extract, 25 parts of anhydrous ethanol and 75 parts of water; the mass ratio of Camellia oleifera extract and Camellia oleifera extract is 1:1.

[0054] Hair Care Essence 2:

[0055] In terms of mass parts, the raw material composition is: 0.72 parts of Paris polyphylla extract, 0.18 parts of Polygonum multiflorum extract, 0.16 parts of Eclipta prostrata extract, 0.18 parts of Ligustrum lucidum fruit extract, 0.09 parts of Angelica sinensis extract, 0.17 parts of Panax ginseng extract, 0.07 parts of clove extract, 0.07 parts of Selaginella tataricus extract, 0.23 parts of Camellia oleifera extract, 0.09 parts of Cinchona quinata extract, 24 parts of anhydrous ethanol and 77 parts of water; the mass ratio of Camellia oleifera extract and Camellia oleifera extract is 1:1.44.

[0056] Hair Care Essence 3:

[0057] The raw material composition is as follows in parts by mass: 0.15 parts of Polygonum multiflorum extract, 0.2 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.15 parts of Panax ginseng extract, 25 parts of anhydrous ethanol and 75 parts of water.

[0058] Hair Care Essence 4:

[0059] The raw material composition is as follows in parts by mass: 0.08 parts of angelica extract, 0.08 parts of clove extract, 0.08 parts of Selaginella extract, 25 parts of anhydrous ethanol and 75 parts of water.

[0060] Hair Care Essence 5:

[0061] The raw material composition is as follows in parts by mass: 0.2 parts of nephrolepis chinensis extract, 0.08 parts of cinchona quinquefolia extract, 25 parts of anhydrous ethanol and 75 parts of water.

[0062] Hair Care Essence 6:

[0063] In terms of mass, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.4 parts of Camellia ternata extract, 0.08 parts of Cinchona pubescens extract, 25 parts of anhydrous ethanol and 75 parts of water.

[0064] Hair Care Essence 7:

[0065] In terms of mass, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.4 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.08 parts of Cinchona quinata extract, 25 parts of anhydrous ethanol and 75 parts of water.

[0066] Hair Care Essence 8:

[0067] In terms of mass parts, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.08 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.32 parts of Camellia oleifera extract, 0.08 parts of Cinchona quinata extract, 25 parts of anhydrous ethanol and 75 parts of water; the mass ratio of Camellia oleifera extract and Camellia oleifera extract is 0.25:1.

[0068] Hair Care Essence 9:

[0069] In terms of mass parts, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.11 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.29 parts of Camellia ternata extract, 0.08 parts of Cinchona pubescens extract, 25 parts of anhydrous ethanol and 75 parts of water; the mass ratio of Camellia ternata extract and Camellia ternata extract is 1:2.64.

[0070] Hair Care Essence 10:

[0071] In terms of mass parts, the raw material composition is: 0.15 parts of Polygonum multiflorum extract, 0.2 parts of Eclipta prostrata extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.2 parts of Camellia oleifera extract, 0.08 parts of Cinchona quinata extract, 25 parts of anhydrous ethanol and 75.7 parts of water; the mass ratio of Eclipta prostrata extract and Camellia oleifera extract is 1:1.

[0072] Hair Care Essence 11:

[0073] In terms of mass, the raw material composition is: 0.7 parts of Paris polyphylla extract, 0.15 parts of Polygonum multiflorum extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.08 parts of Cinchona quinquefolia extract, 25 parts of anhydrous ethanol and 75.4 parts of water.

[0074] Hair Care Essence 12:

[0075] The raw material composition is as follows in parts by mass: 0.15 parts of Polygonum multiflorum extract, 0.15 parts of Ligustrum lucidum fruit extract, 0.08 parts of Angelica sinensis extract, 0.15 parts of Panax ginseng extract, 0.08 parts of clove extract, 0.08 parts of Selaginella tataricus extract, 0.08 parts of Cinchona quinquefolia extract, 25 parts of anhydrous ethanol and 76.1 parts of water.

[0076] The preparation method of the hair care essence is as follows: water and anhydrous ethanol are added into a container, stirring is started, the speed is adjusted to 85 rpm, and then various extracts are added, stirred for 0.5 hours, sterilized, and the hair care essence is obtained.

[0077] Tyrosinase is a key rate-limiting enzyme in the process of melanin synthesis, which directly affects the synthesis and synthesis efficiency of melanin. Modern medical theory believes that the cause of white hair is mainly due to the loss of pigment in the hair shaft and the loss or reduction of tyrosinase activity in the hair bulb. Therefore, by properly activating tyrosinase with suitable substances, the synthesis of melanin can be restored to normal, and white hair can be turned into black hair. Therefore, the present invention uses the activation rate of tyrosinase to characterize the effect of black hair.

[0078] The test method of tyrosinase activation rate refers to the literature (Yu Q, Fan L, Duan Z, Fiveindividual polyphenols as tyrosinase inhibitors: Inhibitory activity, synergistic effect, action mechanism, and molecular docking, Food Chem 2019; 297: 124910; Yang Y, Sun X, Ni H, et al, Identification and characterization of the tyrosinase inhibitory activity of caffeine from Camellia pollen, J AgrFood Chem 2019; 67(46): 12741-12751.) was used to determine the tyrosinase inhibitory activity with appropriate modifications. 40 μL tyrosinase solution, 140 μL PBS solution, and 20 μL substrate were added to the 96-well plate in sequence and mixed well as experimental group 1; 180 μL PBS solution and 20 μL substrate were added to the 96-well plate in sequence and mixed well as experimental group 2; 40 μL tyrosinase solution, 40 μL inhibitor solution, 100 μL PBS solution, and 20 μ substrate were added to the 96-well plate in sequence and mixed well as experimental group 3; 40 μL inhibitor solution, 140 μL PBS solution, and 20 μL substrate were added to the 96-well plate in sequence and mixed well as experimental group 4. Three parallel control groups were set up for each group of samples, incubated at 30 °C for 30 min, and then mixed and the absorbance of the solution at 475 nm was detected by a microplate reader for 30 min to calculate the tyrosinase activation rate. Tyrosinase activation rate (%) = 100% × {(CD) / (AB)-1}; where A: absorbance of experimental group 1; B: absorbance of experimental group 2; C: absorbance of experimental group 3; D: absorbance of experimental group 4.

[0079] Before testing, the essence was diluted to a mass concentration of 2% using deionized water, and then the activation rate of tyrosinase was tested according to the above method. The results are shown in Table 1.

[0080] Table 1: Efficiency of hair care essences in activating tyrosinase

[0081] Serial number Tyrosinase activation rate / % Hair Essence 1 84.32±5.48 Hair Essence 2 83.14±6.02 Hair Essence 3 55.63±5.99 Hair Essence 4 35.84±4.75 Hair Essence 5 38.73±5.07 Hair Essence 6 78.65±4.58 Hair Essence 7 76.39±7.24 Hair Essence 8 82.25±4.92 Hair Essence 9 81.07±9.13 Hair Essence 10 61.78±8.36 Hair Essence 11 63.92±7.61 Hair Essence 12 52.46±6.85

[0082] Combined with the test results in Table 1, it can be seen that the active ingredients of the black hair composition of the present invention can promote the activation of tyrosinase, effectively promote the production of melanin, and give the effect of turning white hair into black.

[0083] It can be seen from the test results of hair care essences 1-2 and 3-5 that the combination of the Paris polyphylla extract, Polygonum multiflorum extract, Eclipta prostrata extract, Ligustrum lucidum fruit extract, Angelica sinensis extract, Panax ginseng extract, Clove extract, Selaginella tataricus extract, Camellia ternata extract and Cinchona pubescens extract of the present invention can make the prepared product have a better effect of activating tyrosinase.

[0084] It can be seen from the test in Table 1 that the hair essence 1, which uses 0.2 parts of Eclipta prostrata extract and 0.2 parts of Herba Sinensis extract, has a better tyrosinase activation effect than the hair essence 6 (only 0.4 parts of Herba Sinensis extract is used without Eclipta prostrata extract). And the hair essence 1 has a better tyrosinase activation effect than the hair essence 7 (only 0.4 parts of Eclipta prostrata extract is used without Herba Sinensis extract). The above conclusions can explain that the use of Eclipta prostrata extract and Herba Sinensis extract in the essence system of the present invention produces a synergistic effect in increasing the activity of tyrosinase. The combination of the two can promote each other to increase the activity of tyrosinase and produce melanin more efficiently; the reason for the analysis may be that the combination of the two enriches the types and contents of active ingredients that can activate tyrosinase, making the hair essence more efficient in activating tyrosinase. The mass ratio of Eclipta prostrata extract to Herba Sinensis extract in hair essences 8-9 is not within the range of 1:0.8-1.5, and the effect of the essence on activating tyrosinase is poor.

[0085] From the test of hair essence 1 and hair essence 10 in Table 1, it can be seen that after omitting the Paris polyphylla extract, the tyrosinase activation effect of the hair essence becomes worse. Compared with hair essence 1, hair essence 11 lacks Eclipta prostrata extract and Herba spatholobi extract, and hair essence 12 lacks Eclipta prostrata extract, Herba spatholobi extract and Paris polyphylla extract. The tyrosinase activation effects of the obtained hair essences 11 and 12 are worse than those of hair essence 1.

[0086] 3. Application Examples

[0087] 30 volunteers aged 30 to 60 were recruited to use the above-mentioned hair care essence 1. Then, according to the use situation, the use experience questionnaire was summarized and the use experience was evaluated, specifically: 30 subjects used the above-mentioned hair care essence 1 for a total of 28 days in a row, 24 people felt that the product had a refreshing Chinese medicine flavor and was easy to absorb after use; 24 people felt that the scalp was more comfortable after use; 25 people thought that the product had the effect of relieving itching and dandruff after use; 23 people thought that the product had the effect of preventing hair breakage after use; 24 people thought that the product had the effect of hair care after use; 24 people thought that the product had the effect of soothing after use; 26 people thought that the product could maintain the scalp after use; 27 people felt that the product had the effect of strengthening hair and preventing hair loss after use.

[0088] The invention utilizes a reasonable combination of traditional Chinese medicine ingredients, has better safety and has a good activation effect on tyrosinase; the raw materials and dosage of the composition of the invention are reasonably matched, and the problem of graying hair is solved from the root.

[0089] Those skilled in the art may make various changes and modifications to the present invention. If these changes and modifications fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include these changes and modifications.

Claims

1. A black hair composition containing a Polygonum multiflorum extract, characterized in that: The composition is as follows, by weight: 0.5-1 parts of Paris polyphylla extract, 0.1-0.25 parts of Polygonum multiflorum extract, 0.1-0.25 parts of Eclipta prostrata extract, 0.1-0.25 parts of Ligustrum lucidum fruit extract, 0.05-0.1 parts of Angelica sinensis extract, 0.1-0.25 parts of Ginseng extract, 0.05-0.1 parts of Clove extract, 0.05-0.1 parts of Selaginella thunbergii extract, 0.05-0.25 parts of Herba Cynoglossi extract and 0.05-0.1 parts of Cinchona quinata extract; The preparation method of the Paris polyphylla extract comprises: crushing Paris polyphylla, adding water, heating, ultrasonic-assisted extraction, obtaining an extract, filtering, concentrating the filtrate, and drying to obtain the Paris polyphylla extract; The mass ratio of the Eclipta prostrata extract to the Herba Cynanchum sphaerocarpa extract is 1:0.8-1.5; The method for preparing the black hair composition comprises the step of mixing various extracts.

2. The black hair composition containing the Paris polyphylla extract according to claim 1, characterized in that: The heating is heating to 40-60°C.

3. The black hair composition containing the Paris polyphylla extract according to claim 2, characterized in that: The amount of water added is 8-20 times the mass of the crushed Yunnan Paris polyphylla.

4. The black hair composition containing the Paris polyphylla extract according to claim 2, characterized in that: The ultrasound-assisted extraction time is 20-120 minutes.

5. The black hair composition containing the Paris polyphylla extract according to claim 1, characterized in that: The preparation method of the Polygonum multiflorum extract comprises: crushing the Polygonum multiflorum, adding an extraction solvent according to a solid-liquid ratio of 1:7-18, heating to 45-62° C., performing ultrasonic-assisted extraction for 25-80 minutes to obtain an extract, filtering, concentrating the filtrate, and drying to obtain the Polygonum multiflorum extract.

6. The black hair composition containing the Paris polyphylla extract according to claim 5, characterized in that: The extraction solvent is an ethanol aqueous solution with a volume concentration of 10-25%.

7. The method for preparing a black hair composition containing the Paris polyphylla extract according to any one of claims 1 to 6, characterized in that: The method comprises the step of mixing various extracts.

8. A hair care essence, characterized in that: The black hair composition comprises the black hair composition according to any one of claims 1 to 6 and 20 to 30 parts by mass of anhydrous ethanol and 70 to 80 parts by mass of water.