A quick preservation method for swida macrophylla germplasm

Abstract

The application discloses a kind of mountain yellow skin germplasm quick preservation method, belong to agricultural planting technical field.The method includes the following steps: (1) stock tree is retracted once;(2) germplasm scion grafting;(3) wipe tip and promote new shoot;(4) pruning modeling;(5) stock tree is retracted twice;(6) water and fertilizer management;(7) disease and insect pest control.The mountain yellow skin germplasm quick preservation method of the application is simple, labor-saving, grows fast, grafting survival rate is high, crown is easy to form, can promote early fruiting, reduces production cost.By the method, mountain yellow skin germplasm preservation rate is high, shortens innovation utilization time, effectively improves the preservation capacity of mountain yellow skin germplasm, improves scientific research and breeding efficiency, realizes quick production and utilization, promotes the sustainable development of mountain yellow skin industry.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of agricultural planting technology, and particularly relates to a rapid preservation method of a mountain yellow skin germplasm. BACKGROUND

[0002] The mountain yellow skin is commonly known as chicken skin fruit, is a perennial evergreen shrub or small tree, and mainly distributes in the south subtropical region south of the Tropic of Cancer. The mountain yellow skin fruit is a summer fruit, and is one of special, rare and excellent fruits in China. The fruit of the mountain yellow skin is a berry, and the pericarp and pulp are edible, sweet and sour and fragrant; the pulp contains 17 kinds of amino acids, the total content of which is 1.029 grams per 100 grams, the content of vitamin C is 66-82 milligrams, the total sugar content is 9.8-10%, the solid content is 17.5-18.3%, the protein content is 1.3%, the total acidity is 2.28%, the edible rate is more than 75-80%, the drying rate is 11-14%, and in addition, the mountain yellow skin is rich in iron, calcium and various nutritional elements, and has high nutritional value. The branches, leaves, fruits and rhizomes of the mountain yellow skin have the effects of eliminating phlegm, regulating qi, eliminating stagnation and accumulation, and have good health care effects, are loved by consumers, and have broad market prospects.

[0003] The mountain yellow skin has a unique taste and fragrance in the Rutaceae fruit, and can be processed into mountain yellow skin dried fruit, preserved fruit, fruit jam, beverage and other products. The products have the effects of eliminating stagnation and opening the appetite, and have unique flavors. The mountain yellow skin fruit has good nutritional value and medicinal effects, is a meat seasoning product, is loved by the public, and has gone out of the country. In the past ten years, with the development and utilization of wild mixed fruits, the mountain yellow skin has been developed and utilized as a precious spice fruit, and with the improvement of people's living standards and the development of border tourism, the mountain yellow skin wild fruit will have good development prospects with planned development.

[0004] As a characteristic fruit tree resource, the mountain yellow skin is the largest production area in China, and distributes in Longzhou County, Jiangzhou District, Ningming County, Daxin County and Pingxiang City, wherein the mountain yellow skin production area in Longzhou County accounts for about 60% of Chongzuo City, and the wild mountain yellow skin resource is very rich. However, the development and utilization of the mountain yellow skin is slow at present, and the wild and semi-wild states are distributed, and in addition, the wild resource is continuously reduced due to long-term blind cutting and predatory picking, and a large number of excellent germplasms are lost. The conventional germplasm resource preservation method is to graft branches on seedlings in a nursery, and the seedlings are transplanted to a field for planting after reaching a seedling out nursery standard, and the flowering and fruiting of the germplasm needs 4-5 years, and the cycle is long and the investment cost is high. SUMMARY

[0005] In view of the above problems, the application provides a kind of quick preservation method for the germplasm of Melia toosendan, which adopts secondary retraction, grafting of multiple germplasms, timely pruning to promote sprouting, pruning and shaping, water and fertilizer management and pest control, etc., to realize rapid sprouting of new shoots and the formation of tree crown, effectively improve the preservation ability of the germplasm resource of Melia toosendan, make the preservation rate of the germplasm of Melia toosendan high, shorten the time of innovative utilization, improve the efficiency of scientific research and breeding, and realize the sustainable development of Melia toosendan industry.

[0006] The application is realized by the following technical solutions:

[0007] A kind of quick preservation method for the germplasm of Melia toosendan, comprising the following steps:

[0008] (1) First retraction of stock tree: for the grafted stock tree of Melia toosendan, first retraction is performed at a height of 60-80 cm from the ground, 3-4 main branches and auxiliary main branches growing obliquely and distributed uniformly are selected and retained, and 1-2 straight branches are retained without retraction above the branch mouth by 20-30 cm;

[0009] (2) Grafting of germplasm scion: the introduced germplasm scion of Melia toosendan is grafted at the sawn joint by cutting grafting, one scion is grafted per branch, the joint and the cross section are sealed with grafting film after grafting, and are bound and tightened; and a V-shaped small opening is cut below the joint cross section on the opposite side of the grafting joint to facilitate water flow out and prevent the branch from turning black;

[0010] (3) Pruning to promote new shoots: the shoots sprouting from the lower part of the grafting joint are timely pruned and removed to promote the sprouting of the branch of the scion, new shoots sprout after 20-30 days of grafting, and the well-grown new shoots are selected and retained as new branches when the new shoots sprout to a length of 20-30 cm; after the first time of the sprouting branch of the grafted new shoots matures, wall paint is applied to the sun-facing side of the stock branch to prevent the main branch of the stock from being cracked by the sun;

[0011] (4) Pruning and shaping: when the length of the new branches reaches 30-40 cm, the branches are topped to promote the secondary sprouting of the branches, 2 branches are retained per branch, the branches are topped when the length reaches 30-40 cm, and the branches generate small branches again, which are recycled to make the grafted Melia toosendan tree form a tree crown quickly and bloom and bear fruit early;

[0012] (5) Second retraction of stock tree: after the second time of the sprouting branch of the scion matures, the straight branches of the stock tree that have not been retracted from the branch mouth in the first retraction are sawn flat;

[0013] (6) Water and fertilizer management: the rootstock tree of the Knema japonica is watered once 3-4 days before grafting, and watered once 7-10 days after grafting; the scion is fertilized for the first time after the first branch of the grafted scion is mature; 0.5-1.0 kg of urea or compound fertilizer per plant is applied in a ditch or by watering; then, when the leaves of the new shoots turn from yellow to green, 0.5-1 kg of compound fertilizer per plant is applied in a ditch and mixed with the soil before backfilling; when 30-50% of the new shoots grow to 5-10 cm, foliar fertilizer is sprayed;

[0014] (7) Disease and pest control: when the new shoots grow to 5-10 cm, the conventional disease and pest prevention is carried out together with the foliar fertilizer.

[0015] As a preferred technical solution, the step (1) of the rootstock tree is selected to be retracted once in October-November.

[0016] As a preferred technical solution, the grafting cut in the step (2) is on both sides or the upper part of the section.

[0017] As a preferred technical solution, the step (3) of the wall paint is applied to the length of the rootstock branch on the sunny side, which is 20-30 cm from the grafting interface to the main trunk bifurcation.

[0018] As a preferred technical solution, the step (3) is that the new shoots sprout 4-5 times a year, forming 60-100 small branches, so that the grafted Knema japonica tree quickly forms a canopy and flowers and bears fruit early.

[0019] As a preferred technical solution, during the grafting process, 1-2 scions of the Knema japonica germplasm variety can be grafted on the same rootstock tree, and the marks are made.

[0020] As a preferred technical solution, the (7) disease and pest control mainly includes aphids, leaf miners, thrips, and anthracnose. The compound fertilizer, urea, and foliar fertilizer used in the application are commonly used in the planting of Knema japonica.

[0021] Compared with the prior art, the application has the following advantages and beneficial effects:

[0022] 1. The two-time retraction method adopted in the application selects 3-4 main branches and auxiliary main branches that grow obliquely and are evenly distributed for the first time retraction, and the main branches and auxiliary main branches are cut off 20-30 cm above the branch bifurcation; 1-2 straight branches are not retracted, and the retained branches serve two purposes: one is to ensure the transportation of nutrients and promote the rapid growth of the scion, and the other is to provide shade protection for the grafted branches, thereby improving the survival rate of grafting. The second retraction is to cut off the new shoots after the grafted part grows stably, thereby reducing the competition for nutrients of the new shoots.

[0023] 2. The application cuts a V-shaped small opening below the section on the other side of the grafting interface, so that the water flows out, preventing the branches from turning black, thereby improving the survival rate of the Knema japonica grafting and achieving rapid canopy formation.

[0024] 3, the length of the new branch of the application reaches 30-40 cm, the branches are topped, the secondary shoots are germinated, 2 branches per shoot are left, the length reaches 30-40 cm, the branches are topped, the small branches are regenerated, the crown is quickly formed, the flowering and fruiting in the second year can be guaranteed, and the preservation ability of the mountain yellow skin germplasm resource is effectively improved.

[0025] 4, the grafting branch used in the application is coated with wall paint on the sunny side after the first shoot matures, the main branch is prevented from being cracked by sun exposure, the nutrient transportation is guaranteed, and the growth speed is accelerated.

[0026] 5, the mountain yellow skin germplasm rapid preservation method of the application is simple, labor-saving, fast in growth, easy to form a crown, promotes early fruiting, reduces cost, can effectively improve the preservation ability of the mountain yellow skin germplasm resource, and the mountain yellow skin germplasm can flower and bear fruit in 27-28 months, which is 24-25 months less than the conventional preservation method, and the benefit is improved by 50-60%, effectively solving the problem of high cost of mountain yellow skin germplasm preservation, improving the speed of mountain yellow skin scientific breeding, and realizing the sustainable development of mountain yellow skin industry. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 It is a new shoot diagram of the mountain yellow skin scion.

[0028] Figure 2 It is a mountain yellow skin pinning pruning diagram. DETAILED DESCRIPTION

[0029] The application will be further described in detail through examples, which are only used to illustrate the application and do not limit the protection scope of the application.

[0030] Example 1

[0031] The mountain yellow skin tree germplasm is preserved rapidly by the following method:

[0032] (1) the first back shrinkage of the stock tree: in October and November 2019, the existing ordinary mountain yellow skin tree is selected as the stock tree, the first back shrinkage is carried out at a height of 60-70 cm from the ground, 3-4 main branches and auxiliary main branches growing obliquely and distributed uniformly are selected and left, and a straight main branch is retained without back shrinkage;

[0033] (2) germplasm scion grafting: the collected wild mountain yellow skin scion of germplasm resource is first subjected to conventional sterilization and disinfection, then is subjected to grafting by cutting, 1 scion is grafted on each stock branch, the cut and the section are sealed with grafting sealing film after grafting, and are bound and tightened; and a V-shaped small opening is cut below the section on the opposite side of the scion, so that the water flows out, and the branch is prevented from turning black;

[0034] Two grafts of different SHP germplasm varieties were grafted on the same stock tree, and were labeled. The grafts of SHP germplasm were collected from Nan Ya community in Binqiao Township, Longzhou City, Chongzuo City, and the stock tree was 4 years old. The labels were SHP55 and SHP56.

[0035] (3) Promoting new shoots by removing tips: The branches and shoots extracted from the lower part of the grafting port should be removed in time to reduce the competition for nutrients, so as to promote the germination of the branches of the scion part. New shoots begin to germinate 20-30 days after grafting. When the new shoots grow to 20-25 cm, select the well-grown new shoots as new branches. After the first time of the old mature of the newly germinated branches, use wall paint to smear the stock branches on the sunny side to prevent the main branches of the stock from being cracked by the sun; the length of smearing is 20-30 cm from the grafting port to the main stem bifurcation.

[0036] (4) Pruning and shaping: When the length of the new branches reaches 30-35 cm, the branches are topped to promote the germination of the second shoots, and 2 branches are left per shoot. When the length of the branches reaches 30-40 cm, the branches are topped again to generate small branches. This cycle is repeated to quickly form a tree canopy for the grafted SHP tree and to early flowering and fruiting. The new shoots germinate 4-5 times a year to form 60-100 small branches, which quickly form a tree canopy for the grafted SHP tree and early flowering and fruiting.

[0037] (5) Second shrinkage of stock tree: After the second shoot of the grafted branches is mature, the stock tree is subjected to a second shrinkage, and the straight branches on the stock tree that have not been shrunk in the first shrinkage are sawn flat at the branch bifurcation.

[0038] (6) Water and fertilizer management: The SHP stock tree is watered once 3-4 days before grafting and once 8-10 days after grafting. The first fertilization is carried out after the first time of the old mature of the grafted branches, and 1.0 kg of urea is applied per plant. After that, when the leaves of the new branches turn from yellow to green, a ditch is dug and 0.5 kg of compound fertilizer is applied per plant, which is mixed with the soil and then backfilled. When more than 30-40% of the new shoots grow to 5-10 cm, foliar fertilizer is sprayed.

[0039] (7) Disease and pest control: When the new shoots grow to 5-10 cm, routine disease and pest prevention is carried out together with foliar fertilizer. Disease and pest control mainly includes aphids, leaf miners, thrips and anthracnose.

[0040] Example 2

[0041] The method for rapidly preserving SHP germplasm comprises the following steps:

[0042] (1) First shrinkage of stock tree: In October 2020, existing ordinary SHP trees were selected as stock trees, and the first shrinkage was carried out at a height of 70-80 cm from the ground. 3-4 main branches and auxiliary main branches growing obliquely and evenly distributed were selected and cut off 20-25 cm above the branch bifurcation, and 1 straight branch was left unshrunk.

[0043] (2) The collected wild wild mountain yellow skin scion of germplasm resources is first sterilized and disinfected, and then grafted by cutting method, 1 scion is grafted on each branch of the stock, the grafting sealing film is used to seal the cut and section after grafting, and it is bound and tightened; and a V-shaped small opening is cut below the section on the opposite side of the scion, so that the water flows out, preventing the branches from turning black;

[0044] Two scions of mountain yellow skin germplasm varieties are grafted on the same stock tree, and are well marked. The mountain yellow skin germplasm scions are collected in Jinming Village, Shangjin Township, Longzhou County, Chongzuo, and the stock tree age is 5 years, marked as SHP57 and SHP58.

[0045] (3) Promote new shoots by pruning: the branches and shoots extracted from the lower part of the grafting port should be cut off and removed in time to reduce the competition for nutrients, so as to promote the germination of the branch of the scion part, and the new shoots begin to germinate 20-30 days after grafting, and when the new shoots grow to 20-25 cm, the well-grown new shoots are selected as new branches. After the first pruning of the newly grown branches, the wall paint is applied to the sunny side of the stock branch to prevent the main branch of the stock from being cracked; the length of the applied paint is 20-30 cm from the grafting port to the main stem bifurcation.

[0046] (4) Pruning and shaping: when the length of the new branches reaches 35-40 cm, the branches are topped to promote the germination of the second shoots, and 2 branches are left on each shoot, which are topped when the length reaches 30-40 cm, and the branches regenerate small branches, which are recycled to make the grafted mountain yellow skin tree form a canopy quickly and bloom and bear fruit early. The new shoots sprout 4-5 times a year, forming 60-100 small branches, so that the grafted mountain yellow skin tree forms a canopy quickly and blooms and bears fruit early.

[0047] (5) Second shrinkage of stock tree: after the second shoot of the scion sprouts, the stock tree is subjected to a second shrinkage, and the straight branches on the stock tree that have not been shrunk in the first shrinkage are sawn flat at the branch bifurcation;

[0048] (6) Water and fertilizer management: the mountain yellow skin stock tree is watered once 3-4 days before grafting, and once 8-10 days after grafting; the first fertilization is carried out after the first shoot of the grafted scion is mature, and 1.0 kg of compound fertilizer is applied per plant; thereafter, when the leaves of each batch of new shoots turn from yellow to green, a ditch is dug and 0.5 kg of compound fertilizer is applied per plant, which is mixed with the soil and then backfilled; when 30-40% of the new shoots grow to 5-10 cm, foliar fertilizer is sprayed;

[0049] (7) Disease and pest control: when the new shoots grow to 5-10 cm, the conventional disease and pest prevention is carried out together with the foliar fertilizer. The disease and pest control mainly includes aphids, leaf miners, thrips and anthracnose.

[0050] Example 3

[0051] The application relates to a rapid preservation method of sapwood of Melia toosendan Sieb. et Zucc.

[0052] (1) once back bending of stock trees: in November 2022, existing common Melia toosendan Sieb. et Zucc. trees are selected as stock trees, first back bending is carried out at a height of 60-70 cm from the ground, 3-4 main branches and auxiliary main branches which grow obliquely and are uniformly distributed are selected and reserved, and 1 straight big branch is reserved without back bending, and the straight big branch is sawn off at a position 25-30 cm above the branch mouth;

[0053] (2) the collected sapwood of wild Melia toosendan Sieb. et Zucc. is first subjected to conventional sterilization and disinfection, then is grafted by using a cutting grafting method, 1 grafting piece is grafted on each stock branch, the grafting piece is sealed after grafting, the cutting surface is sealed by using a grafting sealing film, and is tightly bound and tied; and a V-shaped small opening is cut below the cutting surface on the opposite side of the grafting piece, so that water flows out, and the branch is prevented from turning black;

[0054] 2 grafting pieces of sapwood of different Melia toosendan Sieb. et Zucc. species are grafted on the same stock tree, and are well marked. The sapwood of Melia toosendan Sieb. et Zucc. is collected from Lingnan Xincun in Longzhou County of Chongzuo and Beishengcun in Shuikou County of Longzhou County, and the tree age is 7 years, and the marks are SHP61 and SHP62.

[0055] (3) smearing of tips to promote new tips: the tips of the branches which are extracted from the lower part of the grafting mouth are timely cut off and removed, so that the nutrition contention is reduced, the branch of the grafting piece is promoted to germinate, new sprouts are germinated after 20-30 days of grafting, and when the new sprouts are extracted and grow to 5-30 cm, the new sprouts which grow well are selected and reserved as new branches. After the first tip of the newly germinated branch is mature, wall paint is used to smear the stock branch on the sunny side, so that the main branch of the stock is prevented from being cracked by the sun; the smearing length is 20-30 cm from the grafting mouth to the branch bifurcation.

[0056] (4) pruning and shaping: when the length of the new branch reaches 30-35 cm, the branch is topped, secondary sprouting is promoted, 2 branches are reserved for each sprout, the length is topped when the branches reach 30-35 cm, and small branches are regenerated from the branches, so that the grafted Melia toosendan Sieb. et Zucc. tree is rapidly formed into a crown, and flowers and fruits are early produced; the new sprout is extracted 4-5 times per year, 60-100 small branches are formed, the grafted Melia toosendan Sieb. et Zucc. tree is rapidly formed into a crown, and flowers and fruits are early produced.

[0057] (5) second back bending of stock trees: when the second sprout of the grafting piece is mature, the straight big branch on the stock tree which has not been back bent in the first back bending is sawn off from the branch bifurcation;

[0058] (6) Water and fertilizer management: the mountain yellow skin stock tree is watered once 3-4 days before grafting, and watered once 7-8 days after grafting; the first fertilization is carried out after the first branch of the scion survives and matures, 0.5 kg of compound fertilizer is applied to each plant in the ditch; thereafter, when the new shoots turn from yellow to green, 1 kg of compound fertilizer is applied to each plant in the ditch, and the soil is stirred evenly and then backfilled; when more than 40-50% of the new shoots grow to 5-10 cm, foliar fertilizer is sprayed;

[0059] (7) Disease and pest control: when the new shoots grow to 5-10 cm, routine disease and pest prevention is carried out in combination with foliar fertilizer. Disease and pest control mainly includes aphids, leaf miners, thrips and anthracnose.

[0060] Comparative Example 1

[0061] The collected wild mountain yellow skin branches are first sterilized and disinfected according to the conventional method, and then grafted on the seedling in the nursery. When the seedling height reaches 60 cm and the branches are strong, the seedlings are transplanted to the field for planting according to the conventional standard. Fertilization and disease and pest control are carried out according to the conventional standard.

[0062] Comparative Example 2

[0063] The difference from Example 1 is only that in step (1), the stock is directly retracted in place at one time, without retaining the upright branches for shading, and the other steps are the same.

[0064] Comparative Example 3

[0065] The difference from Example 1 is only that in step (3), the wall paint is not used to smear the sun-facing branches of the stock, and the original condition is retained, and the other steps are the same.

[0066] The mountain yellow skin germplasm preservation conditions of Examples 1-3 and Comparative Examples 1-3 are recorded in Table 1 below.

[0067] Table 1 Preservation conditions of mountain yellow skin germplasm under different treatments

[0068]

[0069] From Table 1, it can be seen that:

[0070] (1) Comparison of scion survival rate: the scion survival rate of Examples 1-3 treated by the present application is more than 85%, and the scion survival rate of Comparative Examples 1-3 is less than 60%. The survival rate is 15-25% higher than that of the once-retracted comparative example 2 and 10-20% higher than that of the comparative example 3 without sunscreen treatment. The survival rate is 15-25% higher than that of the once-retracted comparative example 2 and 10-20% higher than that of the comparative example 3 without sunscreen treatment. The present application adopts a two-time retraction method, and retaining the shading branches for the first retraction is beneficial to improving the survival rate of the scion, and using wall paint to smear the sun-facing branches of the stock can also alleviate the withering of the branches of the stock before the survival of the scion, which are all beneficial to improving the survival rate of the scion.

[0071] (2) Flowering and fruiting period comparison, the flowering period of examples 1-3 treated by the method of the application is generally about 27-28 months, and the fruiting period is 28-29 months. For the traditional nursery seedling grafting method (comparative example 1), the survival rate of the scion grafting is comparable to that of the method of the application. However, the flowering period is long, and needs about 51-52 months; the fruiting period is also long, and needs about 52-53 months. The flowering period and fruiting period of the method of the application are about 23-25 months earlier than those of comparative example 1.

Claims

1. A method for rapid conservation of limonia acidissima germplasm, characterized by, It comprises the following steps: (1) One-time backbending of stock tree: for the stock tree of the grafted mountain yellow bark tree, the first backbending is performed at a height of 60-80 cm from the ground, 3-4 main branches and auxiliary main branches growing obliquely and distributed evenly are selected and reserved, and 1-2 straight large branches are not backbended by sawing off at a position 20-30 cm above the branch mouth; The step (1) of one-time backbending of the stock tree is performed in October-November; (2) Grafting of germplasm scion: the mountain yellow bark germplasm scion to be introduced is grafted at the sawing-off mouth by using cutting grafting, 1 scion is grafted per branch, the cutting mouth and the cross section are sealed with grafting film after grafting, and are bound and tightened; and a V-shaped small mouth is cut below the cutting cross section on the opposite side of the grafting mouth, so as to flow out water and prevent the branch from turning black; During grafting, 1-2 mountain yellow bark germplasm scions of different varieties are grafted on the same stock tree, and are well marked; (3) Tip pruning for promoting new shoots: the shoots drawn out below the grafting mouth are timely cut off and removed, so as to promote the germination of the branch of the scion part, new shoots germinate 20-30 days after grafting, and the well-grown new shoots are selected and reserved as new branches when the new shoots grow to a length of 20-30 cm; after the first time of tip aging of the grafted newly drawn-out branch, wall paint is applied to the stock tree branches on the sunny side, so as to prevent the main branches of the stock tree from being cracked by the sun; The length of the wall paint applied to the stock tree branches on the sunny side in the step (3) is 20-30 cm from the grafting mouth to the branch bifurcation mouth; (4) Pruning and shaping: when the length of the new branches reaches 30-40 cm, the branches are topped to promote the germination of secondary shoots, 2 branches are reserved per shoot, the length of the branches is topped when it reaches 30-40 cm, and the branches generate small branches again, so as to be recycled, so that the grafted mountain yellow bark tree rapidly forms a crown and flowers and bears fruits early; (5) Second backbending of stock tree: after the second time of shoot aging of the scion, the stock tree is backbended for the second time, and the straight large branches on the stock tree which have not been backbended in the first backbending are sawn off at the branch mouth; (6) Water and fertilizer management: the mountain yellow bark stock tree is watered once 3-4 days before grafting, and is watered once 7-10 days after grafting; after the first time of shoot aging of the grafted scion, the first time of fertilization is performed, 0.5-1.0 kg of urea or compound fertilizer is applied per plant, and is applied in a ditch or by drenching; when the leaves of the new shoots change from yellow to green, 0.5-1 kg of compound fertilizer is applied per plant by ditching, is mixed with soil, and is backfilled; when 30-50% of the new shoots grow to a length of 5-10 cm, foliar fertilizer is sprayed; (7) Disease and pest control: when the new shoots grow to a length of 5-10 cm, the conventional disease and pest prevention is performed in combination with foliar fertilizer.

2. The method for rapid conservation of Pothos species germplasm as claimed in claim 1, wherein, The cutting mouth in the step (2) is on both sides or the upper part of the cross section.

3. The method for rapid conservation of Pothos species germplasm as claimed in claim 1, wherein, In the step (3), the new shoots are drawn out 4-5 times per year, 60-100 small branches are formed, the grafted mountain yellow bark tree rapidly forms a crown, and flowers and bears fruits early.

4. The method for rapid conservation of Pothos species germplasm as claimed in claim 1, wherein, The disease and pest control in (7) mainly includes aphids, leaf miners, thrips, and anthracnose.

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