A composition for improving concentration and its preparation method

Abstract

The present invention relates to a composition for improving concentration, which comprises 10-20 parts of dried oil rush bean powder by weight, 1-2 parts of phosphatidylserine, 10-15 parts of vegetable oil, 0.5-1.5 parts of spirulina powder, 80-100 parts of milk powder and 250-300 parts of water. The oil rush bean has a neuroprotective effect and improves concentration by improving the activity of the central nervous system, increasing the level of neurotransmitters, and improving cerebral blood circulation. Phosphatidylserine can improve the function of nerve cells, regulate the conduction of nerve impulses, increase blood supply to the brain, and provide nutrition to the brain. There is a synergistic effect between the oil rush bean and phosphatidylserine, which jointly promotes the improvement of brain function and concentration. The rich antioxidant components in the dried oil rush bean powder improve the stability of phosphatidylserine.

Description

Technical Field

[0001] The present invention belongs to the field of biopharmaceuticals, and particularly relates to a composition for improving concentration and a preparation method thereof. Background Art

[0002] Attention deficit is a neurodevelopmental disorder, and its main characteristics include inattention, hyperactivity, and impulsive behavior. The nutritional status of the brain plays an important role in brain development and function. The lack of nutrients in the neurons of the frontal lobe of the brain easily causes a decrease in neurotransmitter release, and the number of neural connections is small and fragile, resulting in an underdeveloped neural network in the frontal lobe, thus leading to inattention. At present, improving attention usually requires starting from the source and supplementing the nutrients in the neurons of the frontal lobe of the brain.

[0003] Cyperus esculentus, also known as Cyperus esculentus L., tiger nuts, iron water chestnuts, underground walnuts, underground chestnuts, sugar root fruits, ginseng fruits, ginseng beans, and foreign ground chestnuts, etc., belongs to the annual herbaceous plants of the Cyperaceae family. Cyperus esculentus fruits are rich in protein, unsaturated fatty acids, dietary fiber, 16 kinds of amino acids, as well as various trace elements such as potassium, calcium, magnesium, selenium, zinc, and manganese, vitamin E, various vitamins such as vitamin B1, B2, and B5, and sterols, plant polysaccharides, etc. Cyperus esculentus has the effects of preventing three highs diseases, myocardial necrosis, myocardial infarction, arteriosclerosis, cardiovascular diseases, gastric tumors, arthritis, gastric stones, leukemia, diabetes, cataracts, organ senility, bone deformation and other diseases, strengthening liver function, regulating the secretion of adrenaline, promoting the growth and development of infants' bones, treating constipation, protecting the intestines, preventing intestinal tumors, and enhancing human immunity. As a kind of miscellaneous grain with both medicinal and edible uses in China, it is recorded in "New Outline of Chinese Materia Medica" that Cyperus esculentus "the tuber is pungent, sweet, and warm in nature, has the effects of soothing the liver and regulating qi, strengthening the spleen and stomach, and is mainly used to treat rib pain and chest tightness caused by stagnation of liver qi; abdominal distension, stomachache, loss of appetite, less food intake due to spleen deficiency, food retention, and indigestion caused by qi stagnation of the spleen and stomach, etc."

[0004] Phosphatidylserine, also known as compound nervonic acid, abbreviated as PS, is extracted from the residues remaining after extracting oil from natural soybeans. It is an active substance of cell membranes, especially present in brain cells. Its main function is to improve the function of nerve cells, regulate the conduction of nerve impulses, and enhance the memory function of the brain. Because of its strong lipophilicity, it can quickly enter the brain through the blood-brain barrier after absorption, playing a role in soothing vascular smooth muscle cells and increasing blood supply to the brain.

[0005] Phosphatidylserine is known in the prior art to be unstable. Even pure dry powder is prone to high - rate degradation when stored under cold conditions. Phosphatidylserine can be emulsified in water, and it is easily unstable in water due to chemical degradation, temperature, ion concentration, and pH value. Therefore, the common preparation forms of phosphatidylserine are mainly solid preparations.

[0006] Oil - in - water emulsion (O / W) is one of the main means of pharmaceutical preparations. Oral emulsions can improve the bioavailability of bioactive substances, increase the effectiveness of products and the compliance of the population taking them. Therefore, the successful preparation and application of emulsions can not only improve the effectiveness of products, but also reduce raw material costs, thereby increasing the clinical options for phosphatidylserine. Summary of the Invention

[0007] The present invention provides a composition for improving concentration and a preparation method thereof.

[0008] A composition for improving concentration, by weight, comprises 10 - 20 parts of dried Cyperus esculentus powder, 1 - 2 parts of phosphatidylserine, 10 - 15 parts of vegetable oil, 0.5 - 1.5 parts of spirulina powder, 80 - 100 parts of milk powder, and 250 - 300 parts of water.

[0009] Sterols, saponins, alkaloids, and vitamins in Cyperus esculentus have neuroprotective effects, can improve the activity of the central nervous system, and can improve concentration, memory, and cognitive level, and relieve diseases related to the central nervous system by reducing the content of brain acetylcholinesterase, malondialdehyde, and nitrite in the brain. Through research and analysis, cyperol in Cyperus esculentus can cross the blood - brain barrier and act synergistically with phosphatidylserine to affect the central nervous system. Cyperol can improve cerebral blood circulation and stimulate the activity of the central nervous system by increasing the levels of neurotransmitters such as dopamine and 5 - hydroxytryptophan and reducing the activity of nitric oxide synthase in the brain, and ultimately improve concentration.

[0010] Phosphatidylserine improves attention and concentration by optimizing signal transduction within the brain. It has a positive promoting effect on tasks that require high levels of concentration, such as learning, working, and driving. The specific functions of phosphatidylserine are as follows: (1) Affecting neurotransmitter transmission: Phosphatidylserine can influence the chemical message transmission of brain neurotransmitters (especially dopamine), which is crucial for maintaining attention and self-control. By balancing neurotransmitter levels, phosphatidylserine helps keep the brain in a clear and focused state. (2) Promoting brain cell function: Phosphatidylserine is an important component of brain nerve cells and an important source of brain nutrition. It can increase the number of brain synapses, the fluidity of brain cell membranes, and promote glucose metabolism in brain cells, thus making brain cells more active and attention more concentrated. (3) Alleviating stress and fatigue: Phosphatidylserine can promote the synthesis of serotonin in the brain, which is a neurotransmitter that can produce a sense of pleasure and relaxation. Therefore, phosphatidylserine helps alleviate stress and anxiety, improve the emotional state, and thus makes it easier for people to maintain concentration.

[0011] The effects of Cyperus esculentus L. and phosphatidylserine are different, and when used together, they have a synergistic effect and can enhance each other's effects. Phosphatidylserine improves concentration by improving the fluidity of cell membranes, while Cyperus esculentus L. improves concentration by increasing neurotransmitter levels and improving cerebral blood circulation. The combination of the two helps improve concentration, memory, and cognitive function, and has a more significant effect.

[0012] Vegetable oils are mainly composed of unsaturated fatty acids, including linoleic acid and oleic acid. Linoleic acid can synthesize DHA related to brain nutrition in the body. Vegetable oils also contain vitamin E and other trace elements and have antioxidant functions, which can protect the brain from free radical damage. As the oil phase in the emulsion, vegetable oil can be fully mixed with the freeze-dried powder of Cyperus esculentus L. and phosphatidylserine to improve the stability of the freeze-dried powder of Cyperus esculentus L. and phosphatidylserine in the emulsion.

[0013] The vegetable oil is one or more of sunflower oil, Cyperus esculentus L. oil, olive oil, walnut oil, and grape seed oil.

[0014] In the composition for improving concentration of the present invention, phosphatidylserine can also be used as an emulsifier, and the freeze-dried powder of Cyperus esculentus L. can be used as both an emulsifier and a stabilizer. The obtained composition after mixing and homogenization has good stability. Placed in a 10 cm centrifuge tube and centrifuged at 4000 r / min for 5 min, there is no layering or flocculation phenomenon. The freeze-dried powder of Cyperus esculentus L. has strong antioxidant ability, which can prevent the oxidation and rancidity of phosphatidylserine in the emulsion.

[0015] The main components of spirulina powder include protein, amino acids, carbohydrates, vitamins, and minerals, etc. Spirulina powder is rich in 13 vitamins, more than 60 minerals, 20 amino acids, and various unsaturated fatty acids. Spirulina powder can be used as a solid particle emulsifier, acting together with the freeze-dried powder of Cyperus esculentus L. to improve the stability of the emulsion.

[0016] The particle size of the freeze-dried powder of Cyperus esculentus L. is 200 - 250 mesh.

[0017] The particle size of the spirulina powder is 200 - 250 mesh.

[0018] The particle size ranges of the freeze-dried powder of Cyperus esculentus L. and the spirulina powder are the same as the particle size range of the oil droplets in the oil-in-water emulsion. The droplet and solid particle sizes in the emulsion are uniform, which can further increase the emulsion stability of the composition for improving concentration in the present invention.

[0019] The freeze-dried powder of Cyperus esculentus L. is prepared by the following method: (1) Select fresh, insect-free, and mildew-free Cyperus esculentus L., wash and peel it, and soak it in a 2% H2O2 aqueous solution at 35°C for 16 - 24 h, and change the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus L. and place it in a static state at a relative humidity of 80%, a temperature of 30°C, and under light conditions for 48 h; (3) Add water to the Cyperus esculentus L. processed in step (2) according to a solid-liquid mass ratio of 1:10, and obtain Cyperus esculentus L. milk after high-pressure homogenization and grinding with a colloid mill; (4) Freeze-dry and spray-dry the Cyperus esculentus L. milk, and obtain the freeze-dried powder of Cyperus esculentus L. by passing through a 200 - 250 mesh sieve.

[0020] The freeze-dried powder of Cyperus esculentus L. in the present invention has high contents of sterols, saponins, alkaloids, vitamins, soluble sugars, and soluble proteins, and high antioxidant activity. Soluble proteins can act as emulsifiers together with phosphatidylserine. Soluble sugars, insoluble dietary fibers, and starches can be used as stabilizers.

[0021] Soaking Cyperus esculentus L. in a 2% H2O2 aqueous solution at 35°C is equivalent to the imbibition stage before seed germination. At this stage, hydrogen peroxide fully activates the antioxidant enzymes in Cyperus esculentus L., such as superoxide dismutase, peroxidase, and catalase, to improve the antioxidant activity of Cyperus esculentus L. During the static stage after imbibition, Cyperus esculentus L. releases various vitamins and growth factors. At the same time, the contents of amylase and protease increase, hydrolyzing starch into small-molecule water-soluble sugars and hydrolyzing macromolecular proteins into water-soluble proteins. The contents of active substances with neuroprotective effects such as sterols, alkaloids, and vitamins increase under the influence of plant growth factors. If the static time is too short, the contents of amylase and protease are insufficient, and starch and macromolecular proteins have not undergone hydrolysis. If the static time is too long, the root and stem cells of Cyperus esculentus L. divide and increase rapidly, and a large amount of nutrients are consumed, resulting in a reduced edible value.

[0022] The homogenization pressure in the step (3) is 40 MPa.

[0023] The vegetable oil is one or more of sunflower oil, tiger nut oil, olive oil, walnut oil, grape seed oil.

[0024] The composition for improving concentration further comprises 1 - 3 parts by weight of walnut peptide and 1 - 2 parts by weight of taurine.

[0025] Walnut peptide is extracted from plant walnut kernels. By using bio - enzymatic hydrolysis technology, natural walnut protein is successfully converted into active small - molecule peptides; the average molecular weight is less than 1000 Daltons. Walnut peptide is rich in important nutrients for brain cell metabolism, can nourish brain cells and enhance brain function. At the same time, walnut peptide contains more brain - strengthening substances such as aspartic acid, glutamic acid, arginine, etc., which can regulate the normal function of the brain and contribute to the intellectual and memory development of teenagers. Walnut peptide contains abundant glutamic acid, which participates in brain metabolism, increases the content of acetylcholine in the brain, so that the cerebral cortical nerve cells are fully active, achieving the effect of improving concentration and memory. Walnut peptide contains γ - aminobutyric acid, which can produce a calming effect, improve sleep quality, and thus achieve the effect of improving concentration.

[0026] Taurine, also known as β - aminoethanesulfonic acid, is an amino acid converted from sulfur - containing amino acids. The pure product is a colorless or white oblique crystal, odorless. Taurine has stable chemical properties and is insoluble in organic solvents such as ether. It is a sulfur - containing non - protein amino acid and exists in a free state in the body. Although taurine does not participate in protein synthesis, it is closely related to the metabolism of cystine and cysteine. Taurine can promote the development of nerve cells and the growth of nerve cell synapses. It can improve the stability of the nervous system, enhance the signal transmission between nerve cells, and contribute to improving concentration and cognitive ability.

[0027] The composition for improving concentration comprises, by weight, 20 parts of tiger nut freeze - dried powder, 15 parts of tiger nut oil, 1.5 parts of spirulina powder, 1 part of phosphatidylserine, 3 parts of walnut peptide, 2 parts of taurine, 100 parts of milk powder and 300 parts of water.

[0028] A preparation method of a composition for improving concentration, comprising the following steps: (1) adding a formulated amount of phosphatidylserine to vegetable oil and mixing evenly to obtain an oil-phase liquid; (2) adding a formulated amount of freeze-dried Cyperus esculentus powder, milk powder, spirulina powder, walnut peptide and taurine to water and mixing evenly to obtain an aqueous-phase liquid; (3) homogenizing the aqueous-phase liquid, and slowly adding the oil-phase liquid during the homogenization process to obtain an emulsion; (4) performing degassing and deoxidation by the vacuum method to remove oxygen and other gases in the mixed solution; (5) heating the deoxygenated mixed solution to 135 °C, and performing sterilization treatment for 5 s to obtain a composition for improving concentration.

[0029] The homogenization pressure in step (3) is 40 MPa, and the homogenization temperature is 70 °C.

[0030] If the homogenization temperature is too low, the fat is not easily dissolved, and the fat particles are not easily micronized, resulting in a poor emulsification effect. When the homogenization temperature is higher than 70 °C, some proteins in the milk powder are denatured at high temperature, damaging the fat globule membrane, which easily causes protein sedimentation and fat floating and agglomeration. Using a homogenization temperature of 70 °C can not only ensure the dissolution of fat but also avoid protein denaturation at high temperature. The emulsification effect is good, and the prepared emulsion has no precipitation after standing for one week.

[0031] The present invention has the following characteristics: The components act synergistically to jointly promote the improvement of brain function and improve concentration; the antioxidant components in the freeze-dried Cyperus esculentus powder help improve the stability of phosphatidylserine, prevent the oxidation and rancidity of phosphatidylserine, and the freeze-dried Cyperus esculentus powder can be used as an emulsifier to improve the stability of the composition emulsion.

[0032] In the long-term stability experiment, after 6 months of storage, there is no stratification or flocculation phenomenon. Description of the Drawings

[0033] Figure 1 It is a broken line graph of the precipitation rate in the accelerated stability experiment of the product in Experimental Example 1 of the present invention.

[0034] Figure 2 It is a broken line graph of the preservation rate of phosphatidylserine in the storage stability experiment of the product in Experimental Example 2 of the present invention.

[0035] Figure 3 It is a bar graph of the improvement of the attention deficit hyperactivity disorder scale score in Experimental Example 3 of the present invention. Detailed Embodiments

[0036] Example 1

[0037] A. Preparation of dried powder of Cyperus esculentus L.: (1) Select fresh Cyperus esculentus L. without insect damage and mildew. After cleaning and peeling, soak it in an aqueous solution of H2O2 with a mass concentration of 2% at 35 °C for 16 h, and change the aqueous solution of H2O2 every 8 h; (2) Drain the soaked Cyperus esculentus L. and place it at a relative humidity of 80%, a temperature of 30 °C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus L. treated in step (2) at a solid-liquid mass ratio of 1:10, and obtain Cyperus esculentus L. milk after high-pressure homogenization and grinding with a colloid mill; (4) Freeze-dry and spray-dry the Cyperus esculentus L. milk, and obtain the dried powder of Cyperus esculentus L. through a 250-mesh sieve.

[0038] B. Preparation of the composition for improving concentration: (1) Add 1 g of phosphatidylserine to 10 g of Cyperus esculentus L. oil and mix evenly to obtain an oil-phase liquid; (2) Add 18 g of dried powder of Cyperus esculentus L., 1.5 g of spirulina powder, and 80 g of milk powder to 300 ml of water and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method for degassing and deoxidizing to remove oxygen and other gases in the mixed solution; (5) Heat the deoxygenated mixed solution to 135 °C, and keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml per strip.

[0039] Example 2

[0040] A. Preparation of dried powder of Cyperus esculentus L.: (1) Select fresh Cyperus esculentus L. without insect damage and mildew. After cleaning and peeling, soak it in an aqueous solution of H2O2 with a mass concentration of 2% at 35 °C for 24 h, and change the aqueous solution of H2O2 every 8 h; (2) Drain the soaked Cyperus esculentus L. and place it at a relative humidity of 80%, a temperature of 30 °C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus L. treated in step (2) at a solid-liquid mass ratio of 1:10, and obtain Cyperus esculentus L. milk after high-pressure homogenization and grinding with a colloid mill; (4) Freeze-dry and spray-dry the Cyperus esculentus L. milk, and obtain the dried powder of Cyperus esculentus L. through a 200-mesh sieve.

[0041] B. Preparation of the composition for improving concentration: (1) Add 2 g of phosphatidylserine to 15 g of olive oil and mix evenly to obtain an oil-phase liquid; (2) Add 15 g of dried powder of Cyperus esculentus L., 1.5 g of spirulina powder, and 100 g of milk powder to 300 ml of water and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method for degassing and deoxidizing to remove oxygen and other gases in the mixed solution; (5) Heat the deoxygenated mixed solution to 135 °C, and keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml per strip.

[0042] Example 3

[0043] A. Preparation of Cyperus esculentus L. freeze-dried powder: (1) Select fresh Cyperus esculentus L. without insect damage and mildew, wash and peel it, and soak it in a 2% H2O2 aqueous solution at 35°C for 18 h, changing the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus L., place it in an environment with a relative humidity of 80%, a temperature of 30°C, and under light conditions, and let it stand for 48 h; (3) Add water to the Cyperus esculentus L. processed in step (2) at a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus L. soymilk; (4) Freeze-dry and spray-dry the Cyperus esculentus L. soymilk, and pass it through a 200-mesh sieve to obtain the Cyperus esculentus L. freeze-dried powder.

[0044] B. Preparation of the composition for improving concentration: (1) Add 1 g of phosphatidylserine to 10 g of sunflower oil and mix evenly to obtain an oil-phase liquid; (2) Add 18 g of Cyperus esculentus L. freeze-dried powder, 0.5 g of spirulina powder, 100 g of milk powder, 2 g of walnut peptide, and 2 g of taurine to 250 ml of water and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method to remove air and oxygen, removing oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml per strip.

[0045] Example 4

[0046] A. Preparation of Cyperus esculentus L. freeze-dried powder: (1) Select fresh Cyperus esculentus L. without insect damage and mildew, wash and peel it, and soak it in a 2% H2O2 aqueous solution at 35°C for 20 h, changing the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus L., place it in an environment with a relative humidity of 80%, a temperature of 30°C, and under light conditions, and let it stand for 48 h; (3) Add water to the Cyperus esculentus L. processed in step (2) at a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus L. soymilk; (4) Freeze-dry and spray-dry the Cyperus esculentus L. soymilk, and pass it through a 200-mesh sieve to obtain the Cyperus esculentus L. freeze-dried powder.

[0047] B. Preparation of the composition for improving concentration: (1) Add 1 g of phosphatidylserine to 7 g of walnut oil and 8 g of Cyperus esculentus L. oil and mix evenly to obtain an oil-phase liquid; (2) Add 10 g of Cyperus esculentus L. freeze-dried powder, 0.5 g of spirulina powder, 100 g of milk powder, 1 g of walnut peptide, and 1 g of taurine to 250 ml of water and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method to remove air and oxygen, removing oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml per strip.

[0048] Example 5

[0049] A. Preparation of freeze-dried Cyperus esculentus powder: (1) Select fresh Cyperus esculentus without insect damage and mildew, wash and peel it, then soak it in a 2% H2O2 aqueous solution at 35°C for 22 h, and change the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus, place it at a relative humidity of 80%, a temperature of 30°C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus processed in step (2) at a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus milk; (4) Freeze-dry and spray-dry the Cyperus esculentus milk, and pass it through a 230-mesh sieve to obtain the freeze-dried Cyperus esculentus powder.

[0050] B. Preparation of the composition for improving concentration: (1) Add 2 g of phosphatidylserine to 5 g of grape seed oil and 10 g of walnut oil, and mix evenly to obtain an oil-phase liquid; (2) Add 20 g of freeze-dried Cyperus esculentus powder, 1 g of spirulina powder, 100 g of milk powder, 3 g of walnut peptide, and 2 g of taurine to 300 ml of water, and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method for degassing and deoxygenation to remove oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml / strip. Example 6

[0051] A. Preparation of freeze-dried Cyperus esculentus powder: (1) Select fresh Cyperus esculentus without insect damage and mildew, wash and peel it, then soak it in a 2% H2O2 aqueous solution at 35°C for 16 h, and change the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus, place it at a relative humidity of 80%, a temperature of 30°C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus processed in step (2) at a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus milk; (4) Freeze-dry and spray-dry the Cyperus esculentus milk, and pass it through a 200-mesh sieve to obtain the freeze-dried Cyperus esculentus powder.

[0052] B. Preparation of the composition for improving concentration: (1) Add 1 g of phosphatidylserine to 10 g of grape seed oil and 5 g of olive oil, and mix evenly to obtain an oil-phase liquid; (2) Add 15 g of freeze-dried Cyperus esculentus powder, 90 g of milk powder, 1 g of spirulina powder, 2 g of walnut peptide, and 1 g of taurine to 270 ml of water, and mix evenly to obtain an aqueous-phase liquid; (3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method for degassing and deoxygenation to remove oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition for improving concentration. Pack it into strip packages, 30 ml / strip. Comparative Example 1

[0053] A. Preparation of freeze-dried Cyperus esculentus powder: (1) Select fresh Cyperus esculentus without insect damage and mildew. After cleaning and peeling, soak it in an aqueous solution of H2O2 with a mass concentration of 2% at 35°C for 16 h, and change the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus and place it at a relative humidity of 80%, a temperature of 30°C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus treated in step (2) according to a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus milk; (4) Freeze-dry and spray-dry the Cyperus esculentus milk, and pass it through a 250-mesh sieve to obtain the freeze-dried Cyperus esculentus powder.

[0054] B. Preparation of the composition without phosphatidylserine: (1) Weigh 10 g of Cyperus esculentus oil as the oil phase liquid; (2) Add 18 g of freeze-dried Cyperus esculentus powder, 1.5 g of spirulina powder, and 100 g of milk powder to 300 ml of water and mix evenly to obtain the water phase liquid; (3) Homogenize the water phase liquid, and slowly add the oil phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method to remove air and oxygen, and remove oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition without phosphatidylserine. Pack it into strip packages, 30 ml per strip.

[0055] Comparative Example 2

[0056] A. Preparation of the composition without freeze-dried Cyperus esculentus powder: (1) Add 1 g of phosphatidylserine to 10 g of Cyperus esculentus oil and mix evenly to obtain the oil phase liquid; (2) Add 1.5 g of spirulina powder and 100 g of milk powder to 300 ml of water and mix evenly to obtain the water phase liquid; (3) Homogenize the water phase liquid, and slowly add the oil phase liquid during the homogenization process to obtain an emulsion; (4) Use the vacuum method to remove air and oxygen, and remove oxygen and other gases from the mixed solution; (5) Heat the deoxygenated mixed solution to 135°C, keep it for 5 s for sterilization treatment to obtain the composition without freeze-dried Cyperus esculentus powder. Pack it into strip packages, 30 ml per strip.

[0057] Experimental Example 1: Accelerated stability test of the product

[0058] Place the strip packages of the compositions of Examples 1-6 and Comparative Examples 1 and 2 of the present invention at a temperature of 40 ± 2°C and a relative humidity of 75 ± 5% in the dark for 6 months. Take samples once a month, place them in a centrifuge tube, centrifuge at 4000 r / min for 5 min, measure the precipitation therein, and calculate the precipitation rate. The results are shown in Table 1.

[0059] Table 1 Precipitation rates of the compositions of Examples 1-6 and Comparative Examples 1 and 2 of the present invention after centrifugation.

[0060] Group 0 months 1 month 2 months 3 months 4 months 5 months 6 months Example 1 0.00% 0.02% 0.03% 0.04% 0.07% 0.09% 0.14% Example 2 0.00% 0.03% 0.04% 0.06% 0.08% 0.11% 0.15% Example 3 0.00% 0.03% 0.05% 0.08% 0.11% 0.15% 0.17% Example 4 0.00% 0.04% 0.06% 0.07% 0.11% 0.13% 0.15% Example 5 0.00% 0.02% 0.04% 0.07% 0.12% 0.14% 0.16% Example 6 0.00% 0.02% 0.04% 0.07% 0.10% 0.12% 0.15% Comparative Example 1 0.02% 0.18% 0.29% 0.38% 0.45% 0.53% 0.57% Comparative Example 2 0.00% 0.12% 0.21% 0.29% 0.33% 0.36% 0.38%

[0061] As can be seen from Table 1, the emulsion quality of Examples 1-6 of the present invention is stable, and the precipitation rate is far less than that of Comparative Examples 1 and 2. The precipitation rates of Comparative Examples 1 and 2 are higher than those of Examples 1-6. Comparative Example 1 lacks phosphatidylserine as an emulsifier, and Comparative Example 2 lacks the freeze-dried powder of Cyperus esculentus as a stabilizer, indicating that both the freeze-dried powder of Cyperus esculentus and phosphatidylserine play important roles in the emulsion stability of the composition.

[0062] Experimental Example 2: Storage Stability Experiment of Phosphatidylserine in the Product

[0063] Take 10.0 g of the compositions of Examples 1-6 and Comparative Example 2, add 10 ml of chloroform, extract by ultrasonic oscillation, centrifuge to obtain the supernatant, filter through a 0.45 µm filter membrane, inject 10 µl of the sample, and separate and determine by high performance liquid chromatography. Samples are taken once a month. The chromatographic column used is TOSO7147TSKgel silica-60, (4.6 mm ID × 25 cm). The mobile phase is a mixed solution of acetonitrile / methanol / phosphoric acid with a volume ratio of 900 / 95 / 5. The solution is filtered through a 0.45 µm filter membrane after mixing. The flow rate of the mobile phase is 1 ml / min. The temperature of the column oven is 35 °C. Calculate the retention rate of phosphatidylserine. The results are shown in Table 2 below.

[0064] Table 2 Retention rates of phosphatidylserine in the compositions of Examples 1-6 and Comparative Example 2 of the present invention.

[0065] Group 0 months 1 month 2 months 3 months 4 months 5 months 6 months Example 1 100% 99.99% 99.98% 99.59% 99.18% 98.85% 98.13% Example 2 100% 99.96% 99.91% 99.83% 99.29% 98.84% 98.23% Example 3 100% 99.97% 99.85% 99.59% 98.83% 97.54% 96.47% Example 4 100% 99.93% 99.54% 98.61% 97.91% 96.76% 95.89% Example 5 100% 99.97% 99.76% 99.27% 98.96% 98.58% 98.25% Example 6 100% 99.97% 99.86% 99.80% 99.42% 98.59% 98.17% Comparative Example 2 100% 98.84% 98.08% 96.53% 93.25% 89.44% 86.49%

[0066] As can be seen from Table 2, the quality of phosphatidylserine in the compositions for improving concentration prepared in Examples 1-6 is stable, and there is very little oxidative degradation and rancidity during storage. The dosage of the freeze-dried powder of Cyperus esculentus is positively correlated with the retention rate of phosphatidylserine. After 6 months of accelerated stability experiment in Comparative Example 2, the retention rate of phosphatidylserine is only 86.49%, indicating that the presence of the freeze-dried powder of Cyperus esculentus has a great impact on the storage stability of phosphatidylserine in the compositions for improving concentration. The antioxidant components in Cyperus esculentus can prevent the oxidative degradation of phosphatidylserine.

[0067] Experimental Example 3: Clinical Experiment of the Product

[0068] The research objects were 80 children with attention deficit hyperactivity disorder, who were divided into the groups of Examples 1-6 and the groups of Comparative Examples 1 and 2 according to the random number table method. There were 10 people in each group. The legal guardians of the children all signed the patient informed consent form.

[0069] Diagnostic criteria: According to the diagnostic criteria for attention deficit hyperactivity disorder in the Diagnostic and Statistical Manual of Mental Disorders (DSM-V) of the American Psychiatric Association, A-E need to be met. It meets the attention disorder-dominant type but does not meet the hyperactive-impulsive symptoms of type II, with inattention, manifested as low concentration, distractibility, absent-mindedness, forgetfulness, impatience with trivial matters, and susceptibility to frustration.

[0070] Case selection criteria: Meeting the diagnostic criteria for attention deficit hyperactivity disorder in children; aged 6-14 years; able to actively cooperate with treatment and complete all courses; disease duration ≥ 6 months; obtaining informed consent from the families of the children.

[0071] Exclusion criteria: Children with severe abnormalities in the heart, lungs, liver, and kidneys; children with manifestations similar to attention deficit hyperactivity disorder caused by hearing impairment; children with severe organic diseases such as those involving the nervous system or hematopoietic system; children with allergic constitutions; children with mental illnesses.

[0072] Characteristics of the finally included population: 62 male children and 18 female children; aged 6-13 years, with an average age of (9.73 ± 2.89) years. There were no statistically significant differences in the general data of each group (P < 0.05).

[0073] Treatment plan: In the groups of Examples 1-6 and Comparative Examples 1 and 2, the compositions prepared in Examples 1-6, Comparative Example 1, and Comparative Example 2 of the present invention were taken at 30 ml (1 bag) 2 times a day (once before breakfast and once before dinner). Two months was a treatment course.

[0074] The improvement of the attention deficit hyperactivity disorder scale score (SNAP-IV) before and after treatment is shown in Table 3.

[0075] Table 3 Scale scores of attention deficit hyperactivity disorder (SNAP-IV) (Mean ± SD) in the groups of Examples 1-6 and Comparative Examples 1 and 2.

[0076] Group Before treatment After treatment Example 1 group 18.2±2.8 11.4±1.4 Example 2 group 19.1±1.9 10.8±1.2 Example 3 group 19.3±2.7 9.6±1.6 Example 4 group 18.5±1.5 9.3±1.7 Example 5 group 19.2±1.8 9.8±2.2 Example 6 group 18.7±2.3 10.0±2.0 Comparative Example 1 group 18.6±2.4 14.9±2.3 Comparative Example 2 group 18.6±1.6 14.8±2.2

[0077] As can be seen from Table 3, the compositions for improving concentration in the groups of Examples 1-6 of the present invention have obvious effects on improving attention deficit in children. Before treatment, the attention deficit factor was in the severe abnormal range (18-22), and after treatment, the attention deficit factor generally returned to the normal range (0-13 points). In the group of Comparative Example 1 and the group of Comparative Example 2, the attention deficit factor was in the severe abnormal range (18-22) before treatment, and after treatment, the scale score of attention deficit hyperactivity disorder was in the mild abnormal range (14-17 points), and some were in the normal range, with a worse effect on improving concentration than the groups of Examples 1-6. This shows that there is a synergistic effect between tiger nuts and phosphatidylserine. When the two are used simultaneously, it can significantly improve concentration.

Claims

1. A composition for improving concentration, characterized in that: It comprises, by weight parts, 10 - 20 parts of freeze-dried Cyperus esculentus powder, 1 - 2 parts of phosphatidylserine, 10 - 15 parts of vegetable oil, 0.5 - 1.5 parts of spirulina powder, 80 - 100 parts of milk powder and 250 - 300 parts of water. The freeze-dried Cyperus esculentus powder is prepared by the following method: (1) Select fresh, insect-free and mildew-free Cyperus esculentus, wash and peel it, and soak it in a 2% H2O2 aqueous solution at 35°C for 16 - 24 h, and change the H2O2 aqueous solution every 8 h; (2) Drain the soaked Cyperus esculentus, place it at a relative humidity of 80%, a temperature of 30°C, and let it stand still under light conditions for 48 h; (3) Add water to the Cyperus esculentus treated in step (2) according to a solid-liquid mass ratio of 1:10, homogenize it under high pressure, and then grind it with a colloid mill to obtain Cyperus esculentus soymilk; (4) Freeze-dry and spray-dry the Cyperus esculentus soymilk, and sieve it through a 200 - 250 mesh sieve to obtain the freeze-dried Cyperus esculentus powder. The composition for improving concentration is prepared by the following method, including the following steps: (S1) Add the formulated amount of phosphatidylserine to the vegetable oil and mix evenly to obtain an oil-phase liquid; (S2) Add the formulated amounts of freeze-dried Cyperus esculentus powder, milk powder, and spirulina powder to the water and mix evenly to obtain an aqueous-phase liquid; (S3) Homogenize the aqueous-phase liquid, and slowly add the oil-phase liquid during the homogenization process to obtain an emulsion; (S4) Use the vacuum method to degas and deoxygenate to remove oxygen and other gases in the mixed solution; (S5) Heat the deoxygenated mixed solution to 135°C, and keep it for 5 s for sterilization treatment to obtain the composition for improving concentration.

2. The composition for improving concentration according to claim 1, wherein: The particle size of the spirulina powder is 200 - 250 mesh.

3. The composition for improving concentration according to claim 2, characterized in that: The vegetable oil is one or more of sunflower oil, Cyperus esculentus oil, olive oil, walnut oil, and grape seed oil.

4. The composition for improving concentration according to claim 3, characterized in that: The composition for improving concentration further comprises 1 - 3 parts of walnut peptide and 1 - 2 parts of taurine.

5. The composition for improving concentration according to claim 4, characterized in that: The composition for improving concentration, by weight parts, comprises 20 parts of freeze-dried Cyperus esculentus powder, 15 parts of Cyperus esculentus oil, 1.5 parts of spirulina powder, 1 part of phosphatidylserine, 3 parts of walnut peptide, 2 parts of taurine, 100 parts of milk powder and 300 parts of water.

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