An experimental method for researching carbon sink function of closed shellfish culture

Abstract

The application provides a closed type shellfish breeding carbon sink function research experiment method, and relates to the technical field of breeding ecology research devices, and the method comprises the following steps: putting bait microalgae into a microalgae culture pond, starting a light source supplement device, a chlorophyll instrument and a first water pump, and monitoring the chlorophyll concentration in the microalgae culture pond; when the culture concentration of the microalgae reaches a conveying condition, starting an electromagnetic valve, conveying the microalgae liquid to an animal experiment pond; putting carbon isotope labeled filter-feeding shellfish into the animal experiment pond, starting a peristaltic pump and a second water pump after opening for 1 hour, conveying the water in the animal experiment pond to the microalgae culture pond; monitoring the physicochemical environmental parameters of the system by using a multi-parameter real-time monitor, regularly taking water, and monitoring the physicochemical biological parameters of the system. The method realizes the real-time tracking function of the physiological metabolic carbon flow of the filter-feeding shellfish and the real-time monitoring function of the key physicochemical parameters during the experiment.

Description

Technical Field

[0001] This invention relates to the field of aquaculture ecology research equipment technology, and in particular to an experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture. Background Technology

[0002] Filter-feeding shellfish are an important ecological group in the ecosystem. More and more studies have found that filter-feeding shellfish-mediated aquaculture ecosystems have very complex processes and mechanisms for the migration and transformation of different forms of carbon.

[0003] However, current research on the carbon sequestration function of filter-feeding shellfish mainly focuses on individual carbon budgets. The beneficial feedback relationship between shellfish and microalgae is a crucial indicator of their ability to function as carbon sinks, but research in this area is scarce. This is largely due to the lack of effective and reliable experimental equipment to analyze the carbon sequestration function of shellfish farming at the ecosystem level. Therefore, it is essential to design a closed-loop experimental method for studying the carbon sequestration function of shellfish farming. Summary of the Invention

[0004] The purpose of this invention is to provide an experimental method for studying the carbon sink function of closed-loop shellfish aquaculture, so as to achieve real-time tracking of the carbon flow direction of the physiological metabolism of filter-feeding shellfish, and real-time monitoring of key physicochemical parameters during the experiment.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] An experimental system for studying the carbon sequestration function of closed-loop shellfish aquaculture includes: an external connection device, a microalgae culture device, and a multifunctional animal culture device; the external connection device is connected to the microalgae culture device and the multifunctional animal culture device.

[0007] The microalgae cultivation device includes: a microalgae cultivation tank, a light source supplementation device, a chlorophyll meter, a first water pump, and a first valve; the light source supplementation device, the chlorophyll meter, and the first water pump are all built into the microalgae cultivation tank, and the first valve is located outside the microalgae cultivation tank;

[0008] The multifunctional animal culture device includes: an animal experimental pool, a multi-parameter real-time monitor, sensors, a suspension hook, a second valve, a breeding net, and a second water pump; the multi-parameter real-time monitor, sensors, suspension hook, breeding net, and second water pump are all built into the animal experimental pool, and the second valve is located outside the animal experimental pool;

[0009] External connection devices include: a peristaltic pump and a solenoid valve; both the peristaltic pump and the solenoid valve are connected to the microalgae culture tank and the animal experimental tank.

[0010] Optionally, both the microalgae culture tank and the animal experiment tank are equipped with water outlets at the bottom, and both the microalgae culture tank and the animal experiment tank are equipped with support plates and support frames on the outside; the water outlets are used to discharge impurities and shellfish excrement from the microalgae culture tank and the animal experiment tank.

[0011] Optionally, the microalgae culture tank is made of transparent tempered glass, and the animal experiment tank is made of opaque plastic. The upper part of both the microalgae culture tank and the animal experiment tank is cylindrical, and the lower part is conical.

[0012] Optionally, the light source supplementation device is installed at the top of the microalgae cultivation tank, and LED lighting is used, with the light cycle set to 12 hours.

[0013] Optionally, the power of both the first and second water pumps is 10~20W, and the flow rate of the peristaltic pump is 10~100L / h. -1 The first and second water pumps are used for internal circulation of the water in the microalgae culture tank and the animal experiment tank.

[0014] Optionally, both the first valve and the second valve are located 50-70 cm from the top of the microalgae culture tank or animal experiment tank.

[0015] This invention also provides an experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture, applied to the aforementioned experimental system for studying the carbon sequestration function of closed-loop shellfish aquaculture. The experimental method includes the following steps:

[0016] The experimental bait microalgae were placed in the microalgae culture tank, the light source supplementation device was turned on and the light intensity was set. The chlorophyll concentration in the water of the microalgae culture tank was monitored in real time using a chlorophyll meter, and the first water pump was started to circulate the water in the microalgae culture tank internally.

[0017] When the concentration of the food algae in the microalgae culture tank reaches the delivery condition, the solenoid valve is activated to deliver the liquid food algae in the microalgae culture tank to the animal experimental tank.

[0018] Carbon isotope-labeled filter-feeding shellfish were suspended on hooks in the animal experimental pool or placed on the culture net in the animal experimental pool. One hour after the filter-feeding shellfish started feeding, the peristaltic pump was started to transfer the water in the animal experimental pool to the microalgae culture pool, and the second water pump was started to circulate the water in the animal experimental pool.

[0019] The physicochemical environmental parameters of the closed shellfish aquaculture carbon sequestration function research experimental system are monitored in real time using a multi-parameter real-time monitoring instrument. Water is periodically drawn from the first and second valves to continuously monitor the physicochemical and biological parameters of the closed shellfish aquaculture carbon sequestration function research experimental system.

[0020] After the experiment was completed, all the equipment in the closed shellfish aquaculture carbon sequestration function research experimental system was cleaned, and the dead feed algae and filter-feeding shellfish excrement were discharged through the water outlet.

[0021] Optionally, the delivery conditions are: the initial culture density of the food microalgae reaches 2*102 5 ~8*10 5 cells / mL -1 .

[0022] Optionally, the physicochemical and biological parameters include: the growth rate of the food microalgae, the carbon isotope-labeled density of the microalgae, and the carbon dioxide concentration in the water.

[0023] According to specific embodiments provided by the present invention, the following technical effects are disclosed: The present invention provides a closed-loop carbon sequestration function research experimental system for shellfish aquaculture, comprising: a microalgae culture tank, a light source supplementation device, a chlorophyll meter, a first water pump, a first valve, an animal experimental tank, a multi-parameter real-time monitoring instrument, a sensor, a suspension hook, a second valve, an aquaculture net, a peristaltic pump, and a solenoid valve; the light source supplementation device, the chlorophyll meter, and the first water pump are all built into the microalgae culture tank, the first valve is located outside the microalgae culture tank, the multi-parameter real-time monitoring instrument, the sensor, the suspension hook, the aquaculture net, and the second water pump are all built into the animal experimental tank, and the second valve is located outside the animal experimental tank; the peristaltic pump and the solenoid valve are both connected to the microalgae culture tank and the animal experimental tank. Before the experiment began, the experimental bait microalgae were placed in the microalgae culture tank. The light source supplementation device, chlorophyll meter, and first water pump were activated to monitor the chlorophyll concentration in the water of the microalgae culture tank in real time and to implement internal circulation. When the culture concentration of the bait microalgae in the microalgae culture tank reached the delivery conditions, the solenoid valve was activated to deliver the microalgae liquid to the animal experimental tank. Carbon isotope-labeled filter-feeding shellfish were suspended on hooks in the animal experimental tank or placed on the culture net in the animal experimental tank. One hour after the filter-feeding shellfish began to open their mouths, the peristaltic pump was activated to pump water from the animal experimental tank. The algae were transported to the microalgae culture tank, and the second water pump was activated to circulate the water in the animal experimental tank. The physicochemical environmental parameters of the closed-loop shellfish aquaculture carbon sequestration function research experimental system were monitored in real time using a multi-parameter real-time monitoring instrument. Water was periodically drawn from the first and second valves to continuously monitor the physicochemical and biological parameters of the closed-loop shellfish aquaculture carbon sequestration function research experimental system. After the experiment, all equipment in the closed-loop shellfish aquaculture carbon sequestration function research experimental system was cleaned, and dead feed algae and filter-feeding shellfish feces were discharged through the outlet. This method achieves real-time tracking of the physiological metabolic carbon flow direction of filter-feeding shellfish and real-time monitoring of key physicochemical parameters during the experiment. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the experimental system for studying the carbon sequestration function of closed shellfish aquaculture, according to an embodiment of the present invention.

[0026] Reference numerals: 1. Light source supplementation device; 2. Circular hole; 3. First valve; 4. Chlorophyll meter; 5. First water pump; 6. Microalgae culture tank; 7. Sealing cover; 8. Suspension hook; 9. Second valve; 10. Sensor; 11. Animal experimental tank; 12. Breeding net; 13. Support plate; 14. Support frame; 15. Water outlet; 16. Solenoid valve; 17. Peristaltic pump; 18. Multi-parameter real-time monitoring instrument. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] like Figure 1 As shown, the present invention provides a closed experimental system for studying the carbon sequestration function of shellfish aquaculture, comprising: an external connection device, a microalgae culture device, and a multifunctional animal culture device; the external connection device is connected to the microalgae culture device and the multifunctional animal culture device;

[0030] The microalgae cultivation device includes: a microalgae cultivation tank 6, a light source supplementation device 1, a chlorophyll meter 4, a first water pump 5, and a first valve 3; the light source supplementation device 1, the chlorophyll meter 4, and the first water pump 5 are all built into the microalgae cultivation tank 6, and the first valve 3 is located outside the microalgae cultivation tank 6.

[0031] The multifunctional animal culture device includes: an animal experimental pool 11, a multi-parameter real-time monitor 18, a sensor 10, a suspension hook 8, a second valve 9, a culture net 12, and a second water pump; the multi-parameter real-time monitor 18, the sensor 10, the suspension hook 8, the culture net 12, and the second water pump are all built into the animal experimental pool 11, and the second valve 9 is located outside the animal experimental pool 11; the suspension hook 8 is used to suspend the culture rope of fixed filter-feeding shellfish (such as oysters), the culture net is used to culture attached filter-feeding shellfish (such as mussels), and the multi-parameter real-time monitor 18 is used for real-time monitoring of physicochemical environmental parameters such as water temperature, salinity, and dissolved oxygen in the animal experimental pool 11 during the experiment;

[0032] The external connection devices include a peristaltic pump 17 and a solenoid valve 16; both the peristaltic pump 17 and the solenoid valve 16 are connected to the microalgae culture tank 6 and the animal experiment tank 11.

[0033] Specifically, the total height of the microalgae culture tank 6 and the animal experiment tank 11 is 2.0m, and the total seawater holding volume is 1.2~3m³. 3 Both are equipped with a removable sealing cap 7 at the top, with a 5cm diameter hole 2 and a corresponding rubber stopper for nutrient replenishment during the experiment. The microalgae culture tank 6 is made of transparent tempered glass, while the animal experiment tank 11 is made of opaque plastic. The upper parts of both tanks are cylindrical with a diameter of 1.0~1.5m to facilitate water circulation, while the lower parts are conical to facilitate cleaning after the experiment. Both tanks have outlet holes 15 at the bottom to facilitate the discharge of dead algae, shellfish feces, and other excrement after the experiment.

[0034] Both the microalgae culture tank 6 and the animal experiment tank 11 are externally equipped with support plates 13 and support frames 14; the microalgae culture tank 6 has a built-in light source supplementation device 1, including multiple sets of LED lights installed around and above the tank walls to ensure that the light promoting microalgae growth can reach the depths of the water, with a light intensity of 30~150 μmol / m². -2 s -1 The light-dark cycle is 12 hours during the day and 12 hours at night, and the light intensity can be adjusted according to the type of microalgae.

[0035] Specifically, the power of the first water pump 5 and the second water pump is both 10~20W, and the flow rate of the peristaltic pump 17 is 10~100L / h. -1 The first water pump 5 and the second water pump are used to circulate the water in the microalgae culture tank 6 and the animal experiment tank 11.

[0036] Specifically, the first valve 3 and the second valve 9 are both located 50-70 cm away from the top of the microalgae culture tank 6 or the animal experiment tank 11, for periodic water sampling and measurement of experimental indicators during the experiment.

[0037] This invention also provides an experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture, applied to the aforementioned closed-loop shellfish aquaculture carbon sequestration function research experimental system, and using the Pacific oyster and the small crescent-shaped algae as examples of embodiments of this invention for illustration, including the following steps:

[0038] Place the small crescent-shaped rhomboid algae into the microalgae culture tank 6, turn on the light source supplement device 1, and set the light intensity to 50 μmol / m². -2 s -1 The light-dark cycle is set to 12 hours during the day and 12 hours at night. At the same time, the chlorophyll meter 4 is started to monitor the chlorophyll concentration in the water of the microalgae culture tank 6 in real time. The first water pump 5 is started to circulate the water in the microalgae culture tank 6 internally and ensure that the water is mixed evenly.

[0039] When the culture concentration of *Nyctaginus simonii* in microalgae culture tank 6 reached 2*10 5 ~8*10 5 cells / mL -1 At that time, the solenoid valve 16 is activated to continuously transport the feed microalgae liquid in the microalgae culture tank 6 to the animal experiment tank 11;

[0040] Specifically, the solenoid valve 16 and the peristaltic pump 17 are started and stopped at regular intervals every day, each time for 1 to 2 hours.

[0041] The oyster culture rope is suspended from the hook 8 in the animal experiment tank 11, and carbon isotope-labeled oysters are placed in the tank. The culture density is 1-3 oysters / 10L of seawater. The culture density can be adjusted according to the size of the experimental shellfish and its filtration capacity. After the oysters have been acclimatizing for 1 hour, the peristaltic pump 17 is started to circulate the seawater in the animal experiment tank 11 to the microalgae culture tank 6. The second water pump is started to circulate the water in the animal experiment tank 11 internally to ensure that the water is mixed evenly.

[0042] The physicochemical environmental parameters such as water temperature, salinity and dissolved oxygen in the closed shellfish aquaculture carbon sink function research experimental system are monitored in real time using a multi-parameter real-time monitoring instrument 18 and sensor 10. Water is periodically drawn through the first valve 3 and the second valve 9 to continuously monitor the physicochemical and biological parameters such as the growth rate of *Nyctaginus simonii*, the density of carbon isotope-labeled *Nyctaginus simonii*, and the concentration of carbon dioxide in the water.

[0043] After the experiment was completed, all the devices of the closed shellfish aquaculture carbon sequestration function research experimental system were cleaned, and the dead small crescent algae and the feces of the long oysters were discharged through the water outlet 15.

[0044] Specifically, the power of both the first water pump 5 and the second water pump is 12W.

[0045] The beneficial effects of this invention include:

[0046] 1) It can track the source and destination of carbon in the physiological metabolism of shellfish in real time within a closed system;

[0047] 2) It can provide reliable hardware support for accurately assessing the carbon sequestration efficiency of the "filter-feeding shellfish-microalgae community";

[0048] 3) The system is simple to operate and has strong versatility. It is suitable for the study of carbon sink functions of various types of marine shellfish, such as sessile, attached, and burrowing shellfish, laying the foundation for various experimental needs and making up for the lack of experimental equipment in this field.

[0049] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0050] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. Furthermore, those skilled in the art will recognize that, based on the ideas of the present invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. An experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture, characterized in that, include: The experimental bait microalgae were placed in the microalgae culture tank, the light source supplementation device was turned on and the light intensity was set, the chlorophyll concentration of the water in the microalgae culture tank was monitored in real time by a chlorophyll meter, and the first water pump was started to circulate the water in the microalgae culture tank internally. When the culture concentration of the food microalgae in the microalgae culture tank reaches the delivery condition, the solenoid valve is activated to deliver the food microalgae liquid in the microalgae culture tank to the animal experimental tank. Carbon isotope-labeled filter-feeding shellfish are suspended on hooks in the animal experimental pool or placed on the culture net in the animal experimental pool. One hour after the filter-feeding shellfish open, a peristaltic pump is started to transport the water in the animal experimental pool to the microalgae culture pool, and a second water pump is started to circulate the water in the animal experimental pool. The physical and chemical environmental parameters are monitored in real time by a multi-parameter real-time monitoring instrument, and water is periodically drawn through the first and second valves to continuously monitor the physical, chemical and biological parameters. After the experiment is completed, the device is cleaned and the dead bait algae and the feces of the filter-feeding shellfish are discharged through the water outlet. The light source supplementation device, the chlorophyll meter, and the first water pump are all built into the microalgae cultivation tank, and the first valve is located outside the microalgae cultivation tank; the multi-parameter real-time monitoring instrument, the suspension hook, the aquaculture net, and the second water pump are all built into the animal experiment tank, and the second valve is located outside the animal experiment tank; the peristaltic pump and the solenoid valve are both connected to the microalgae cultivation tank and the animal experiment tank; the bottom of both the microalgae cultivation tank and the animal experiment tank are provided with water outlets, and support plates and support frames are provided outside both the microalgae cultivation tank and the animal experiment tank; the water outlets are used to discharge impurities and shellfish excrement from the microalgae cultivation tank and the animal experiment tank.

2. The experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture according to claim 1, characterized in that, The microalgae culture tank is made of transparent tempered glass, and the animal experiment tank is made of opaque plastic. The upper half of both the microalgae culture tank and the animal experiment tank is cylindrical, and the lower half is conical.

3. The experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture according to claim 1, characterized in that, The light source supplementation device is located at the top of the microalgae cultivation tank, and uses LED lighting with a light cycle of 12 hours.

4. The experimental method for studying the carbon sequestration function of closed-system shellfish aquaculture according to claim 1, characterized in that, The power of both the first and second water pumps is 10~20W, and the flow rate of the peristaltic pump is 10~100L / h. -1 The first water pump and the second water pump are used to circulate the water in the microalgae culture tank and the animal experiment tank.

5. The experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture according to claim 1, characterized in that, Both the first valve and the second valve are located 50-70 cm away from the top of the microalgae culture tank or the animal experiment tank.

6. The experimental method for studying the carbon sequestration function of closed-system shellfish aquaculture according to claim 1, characterized in that, The transport conditions are as follows: the initial culture density of the food microalgae reaches 2*102 5 ~8*10 5 cells / mL -1 .

7. The experimental method for studying the carbon sequestration function of closed-loop shellfish aquaculture according to claim 1, characterized in that, The physicochemical and biological parameters include: the growth rate of the bait microalgae, the carbon isotope-labeled microalgae density, and the carbon dioxide concentration in the water.

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