A plant growth regulating composition and its application

Abstract

The present invention belongs to the field of agricultural technology, and specifically relates to a plant growth regulating composition, comprising tea extract and pollen extract. The present invention combines tea extract and pollen extract for the first time, and the combination of the two has a synergistic effect. The composition not only promotes plant growth, but also improves stress resistance, expands the application scope of tea extract and pollen extract, and provides a new choice for regulating plant growth in agriculture.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and in particular relates to a plant growth regulating composition and application thereof. Background Art

[0002] At present, plant extracts are often used to regulate the growth and development of plants. For example, in patent CN110477054A, pine extract, Chenopodium album extract, Tithonia divaricata extract, Sophora alopecuroides extract, and garlic extract are used to prepare a composite growth regulator; in patent CN104135862B, seaweed extracts are used to regulate the growth of crop plants and / or enhance crop plants; and in patent CN117296869A, an alcohol extract of Pinellia ternata is used to promote plant growth.

[0003] The composition of tea is very complex, mainly including proteins, lipids, carbohydrates, theanine, tea polyphenols, caffeine, organic acids, vitamins and pigments, etc. More than 500 compounds determine its extremely high medicinal effects and nutritional value. Tea extracts are mainly used in the fields of food and medicine, such as the preparation of flavors, the preparation of acne-removing compositions, the preparation of slimming tea and tea wine, etc.

[0004] Bee pollen is a kind of irregular oblate object formed by bees collecting pollen grains from the stamens of flowering plants (nectar plants and pollen source plants) and adding special glandular secretions, nectar and saliva. Bee pollen contains a wide variety of active substances, including active proteins, active peptides, vitamins, flavonoid compounds, etc. Rapeseed pollen has become the most abundant bee pollen in my country because rapeseed is an agricultural economic crop planted on a large scale in my country. Its content of active substances such as bioflavonoids is quite high, and it has the effects of enhancing immunity, anti-aging, beauty, prevention and treatment of cerebrovascular diseases and prevention and treatment of prostate diseases. At present, Chengdu Xinchaoyang has conducted comprehensive exploration and verification of the preparation method and application of pollen extract (CN114600892A, CN113133455A, CN113133454A, etc.).

[0005] At present, there is no relevant report on the combination of tea extract and pollen extract as a plant growth regulator. Summary of the invention

[0006] The purpose of the present invention is to provide a plant growth regulating composition, which uses a tea extract and pollen extract composition as a plant growth regulating active ingredient, expands and enriches the application of the composite combination of tea extract and pollen extract, and plays a role in synergistically promoting plant growth and increasing stress resistance.

[0007] The invention provides a plant growth regulating composition, comprising tea extract and pollen extract.

[0008] In the present invention, the tea leaves are selected from green tea, black tea, white tea, yellow tea, oolong tea and dark tea; and / or the pollen extract is rapeseed pollen extract.

[0009] In the technical solution of the present invention, the tea extract and / or pollen extract refers to the extract of tea and / or pollen extracted by water, and the extraction method includes but is not limited to conventional extraction methods such as leaching, heat reflux, and ultrasonic extraction; the extraction order can be: extracting tea and pollen separately, and then extracting after mixing tea and pollen.

[0010] In the present invention, the tea extract and / or pollen extract is water and / or organic solvent extract.

[0011] In the present invention, the concentration ratio of the tea extract to the pollen extract is 0.001-100:0.001-100; or the mass ratio of the tea extract to the pollen is 1:1-10.

[0012] In some specific embodiments of the present invention, when tea leaves and pollen are extracted separately, the concentration ratio of the tea leaves extract to the pollen extract is 0.001-100:0.001-100.

[0013] Furthermore, the tea extraction method comprises: mixing tea leaves and water at a solid-liquid ratio of 1:5 to 15, extracting at 35° C. to 75° C. for 20 min to 50 min to obtain the tea extract;

[0014] And / or, the pollen extraction method comprises: mixing pollen and water at a solid-liquid ratio of 1:1 to 5, filtering and concentrating to obtain a pollen extract.

[0015] In some specific embodiments of the present invention, when tea leaves and pollen are mixed for extraction, the mass ratio of the tea leaves to pollen is 1:1-10.

[0016] Furthermore, tea leaves, pollen and water are mixed, wherein the solid-liquid ratio of tea leaves, pollen and water is 1:5-15, and the extraction is performed at 50° C.-100° C. for 1 h-3 h, and the mixed extract of tea leaves and pollen is obtained after filtering and concentration.

[0017] The present invention provides an application of the above composition in promoting plant growth.

[0018] In the present invention, the promoting of plant growth includes promoting plant germination, promoting the growth of one or more of roots, stems, leaves or flowers and fruits. For example, promoting the growth of root length, stem thickness, plant height, leaf width, leaf length, leaf number, leaf area, biomass, chlorophyll content, yield, etc.

[0019] In the present invention, the plant growth regulating composition is used as an active ingredient of a growth promoter and can be used alone or in combination with other products.

[0020] In the present invention, the plant growth regulating composition is a natural plant growth promoter.

[0021] The plants described in the present invention include but are not limited to economic crops and food crops, such as tobacco, corn, lettuce, wheat, pepper, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear, apple, etc.

[0022] The so-called "economic crops" are of various types, including but not limited to fiber crops (such as cotton, hemp, etc.), oil crops (such as sesame, peanuts, etc.), sugar crops (such as sugarcane, sugar beets, etc.), hobby crops (tobacco), medicinal crops, dye crops, ornamental crops, fruits (such as citrus, kiwi, cherry, pear, apple, etc.) and other economic crops (such as lettuce, pepper, Chinese cabbage, lettuce, Shanghai green), etc.

[0023] The “food crops” mentioned above include, but are not limited to, cereal crops (wheat, rice, corn), tuber crops (including sweet potatoes, potatoes, etc.) and legume crops (including soybeans, broad beans, peas, mung beans, etc.).

[0024] In the product of the present invention, the plant growth regulating composition can be directly used as a single agent. In order to make it stable and easy to transport and store, it can be made into an agricultural product, for example, by adding auxiliary materials to make a corresponding dosage form. The auxiliary materials can be conventional auxiliary materials in the field, for example, thickeners, dispersants, wetting agents, binders, emulsifiers, stabilizers, solvents, etc.

[0025] On the other hand, the plant growth regulating composition of the present invention can be used as a synergist in combination with foliar fertilizers, water-soluble fertilizers, compound fertilizers, pesticides and the like.

[0026] In the present invention, the dosage form of the agricultural product includes but is not limited to powder, granule, aqueous solution, mother solution or mother powder.

[0027] In the present invention, when used, the single agent or agricultural product prepared from the plant growth regulating composition is used to treat seeds, spray leaves or irrigate roots.

[0028] In the present invention, when the plant growth regulating composition or its product is prepared into a solution for promoting seed germination or plant growth, the concentration of the plant growth regulating composition in the solution can be selected according to actual needs.

[0029] For example, it can be selected from 0.001 to 200 ppm, 0.01 to 100 ppm, 0.01 to 50 ppm, or 0.01 ppm, ... 0.02 ppm, ... 0.03 ppm, ... 0.1 ppm, ... 0.2 ppm, ... 0.3 ppm,

[0030] ......1.0ppm, ......2.0ppm, ......3.0ppm, ......10ppm, ......20ppm, ......30ppm and so on.

[0031] The seed treatment comprises mixing a single agent or agricultural product made of the plant growth regulating composition with the seeds to coat the seeds with the plant growth regulating composition, or soaking the seeds to be germinated with a solution of the plant growth regulating composition.

[0032] In the growth promotion experiment of the present invention, the influence of diseases and insect pests on plant growth has been excluded. Through such an experimental design, the results are sufficient to show that the effect of the plant growth regulating composition on promoting plant growth is not caused by avoiding diseases and insect pests.

[0033] The present invention provides application of the plant growth regulating composition in regulating plant stress resistance.

[0034] Plant stress resistance refers to certain traits that plants possess to resist adverse environments, such as cold resistance, drought resistance, saline-alkali resistance, and high temperature resistance.

[0035] In the present invention, the stress resistance includes but is not limited to salt-alkali resistance, cold resistance, drought resistance, and high temperature resistance.

[0036] The present invention also provides a plant growth promoter, which contains the plant growth regulating composition as an active ingredient.

[0037] The present invention also provides a method for promoting plant growth and / or regulating plant stress resistance using the plant growth regulating composition, that is, applying the plant growth regulating composition to plants.

[0038] Beneficial effects of the present invention: The present invention combines tea extract and pollen extract for the first time, and the combination of the two has a synergistic effect. The composition not only promotes plant growth, but also improves stress resistance, expands the application scope of tea extract and pollen extract, and provides a new choice for regulating plant growth in agriculture. DETAILED DESCRIPTION

[0039] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Example 1

[0041] Weigh a certain amount of green tea leaf powder, add 10 times the volume of soft water, ultrasonicate at 55°C hot water at an ultrasonic frequency of 20kHz for 30 minutes, repeat the above operation twice, separate the solid and liquid, take the aqueous phase, and concentrate it to 1 / 4 of the volume of the filtrate to obtain a green tea extract.

[0042] Example 2

[0043] Weigh a certain amount of rapeseed pollen, add 2 times the volume of soft water, stir for 4 hours at an ultrasonic frequency of 20kHz, and the stirring rate is 3500r / min. Then filter, add chitosan to the filtrate, maintain at 60-80℃ for at least 1 hour, cool and stand, separate the solid and liquid, take the water phase, and concentrate to 1 / 4 of the volume of the filtrate to obtain the rapeseed pollen extract.

[0044] Test Example 1

[0045] This test example tests the effect of soaking seeds with tea extract and pollen extract on the germination and root growth of corn by pot culture.

[0046] 1. Test methods

[0047] (1) Experimental samples

[0048] Green tea leaf extract and pollen extract were prepared with pure water into 1% mother solution (i.e., 10000 ppm mother solution), filtered and sterilized, and then diluted and applied according to the experimental design.

[0049] (2) Experimental design

[0050] There were 9 treatments in total, and each treatment was repeated 5 times. See Table 1 for details.

[0051] Table 1 Experimental design

[0052]

[0053] (3) Experimental steps:

[0054] 1) Culture matrix preparation

[0055] Weigh an equal weight (350 g) of vermiculite sterilized with high-pressure steam at 121° C. for 20 minutes and place it in pots for later use, for a total of 45 pots. Add 200 mL of clean water to each pot and stir thoroughly for later use.

[0056] 2) For each treatment, 100g corn seeds were placed in a culture box. According to the experimental design, the prepared tea extract mother solution was diluted 200,000 times, 20,000 times, 2,000 times, and 200 times to obtain 0.05ppm, 0.5ppm, 5ppm, and 50ppm agents, and the pollen extract was diluted 1,000,000 times, 100,000 times, 10,000 times, and 1,000 times to obtain 0.01ppm, 0.1ppm, 1ppm, and 10ppm agents. 200mL of each prepared agent was added to the culture box with seeds, fully mixed with the seeds, and the seeds were completely immersed in the liquid medicine. The treatment was carried out at room temperature for 24 hours.

[0057] 3) After soaking the corn seeds for 24 hours, rinse them with clean water and sow them into the spare culture medium according to the experimental design, sowing 20 seeds per pot, that is, sowing 100 corn seeds per treatment. The experimental results were investigated after 5 days.

[0058] (4) Survey indicators

[0059] The experimental investigation indicators include: germination rate, root length, root fresh weight, plant height, and plant fresh weight.

[0060] 2. Results Analysis

[0061] (1) Effects of soaking seeds with different extracts on corn germination rate

[0062] As shown in Table 2, under the treatment of clean water, the germination rate of corn is relatively low, only 63.0%; after the treatment of tea extract soaking, the germination rate of corn is significantly improved under the treatment of 5-50ppm, with the highest growth rate reaching 39.68%, but under the treatment of 0.05-0.5ppm, the increase is small, especially under the treatment of 0.05ppm, the germination rate only increased by 3.17%. Similarly, after the treatment of pollen extract soaking, the germination rate of corn increased significantly under the treatment of 1-10ppm, followed by 0.1ppm, and the germination rate increased by 11.11% compared with the clean water control, while under the treatment of 0.01ppm, the germination rate increased by a small margin, and the germination rate increased by 7.94%.

[0063] Table 2 Effect of soaking seeds with different extracts on corn germination rate

[0064]

[0065] (2) Effects of soaking seeds with different extracts on the growth of corn seedlings

[0066] Table 3 shows the growth of the root system and aboveground parts of corn after soaking seeds in different treatments, which is consistent with the trend of germination rate. On the one hand, under the treatment of 5-50ppm tea extract, the root length, root weight, plant height and plant weight of corn were significantly improved compared with the control, and the maximum growth rates reached 26.61%, 26.15%, 13.25% and 26.47% respectively, while the improvement effect was not obvious under the treatment of 0.05-0.5ppm, especially under the treatment of 0.05ppm tea extract, the growth of corn was not much different from the control. On the other hand, under the treatment of 0.1-10ppm pollen extract, the growth of corn was significantly improved by handing over clean water, and the highest growth rates of each index reached 21.77%, 27.69%, 13.25% and 35.29% respectively, while under the treatment of 0.01ppm, there was no significant difference between each index and the control.

[0067] Overall, after soaking seeds with tea extract and pollen extract, the germination rate and seedling growth of corn increased significantly, mainly in the treatment with 5-50ppm tea extract and 1-10ppm pollen extract. However, under lower concentrations (0.05-0.5ppm tea extract and 0.01-0.1ppm pollen extract), the growth-promoting effect of corn was relatively weak.

[0068] Therefore, based on this embodiment, tea and pollen extracts can be compounded at low concentrations to test whether the compounded extracts have a synergistic effect on crop growth.

[0069] Table 3 Effects of soaking seeds with different extracts on the root system and aboveground growth of corn

[0070]

[0071] Test Example 2

[0072] This test example uses a potted culture method to test the effect of soaking seeds after mixing tea extract and pollen extract on corn germination and root growth.

[0073] 1. Test methods

[0074] (1) Experimental samples

[0075] Tea extract (Example 1) and pollen extract (Example 2) were prepared with pure water into 0.1% mother solution (i.e., 1000 ppm mother solution), filtered and sterilized, and then diluted and applied according to the experimental design.

[0076] (2) Experimental design

[0077] According to the results of Experimental Example 1, tea extract and pollen extract have a significant promoting effect on the germination rate and growth of corn. Therefore, based on Experimental Example 1, this experimental example reduces the concentration of the two extracts for compounding and tests the effects of the compounded extracts on the germination and growth of crops.

[0078] There were 4 treatments in total, and each treatment was repeated 5 times. See Table 4 for details.

[0079] Table 4 Experimental design

[0080]

[0081] (3) Experimental steps:

[0082] 1) Culture matrix preparation

[0083] Weigh an equal weight (350 g) of vermiculite sterilized with high-pressure steam at 121° C. for 20 minutes and place it in pots for later use, for a total of 20 pots. Add 200 mL of clean water to each pot and stir thoroughly for later use.

[0084] 2) For each treatment, 100g of seeds were placed in a culture box. According to the experimental design, the prepared tea extract mother solution (1000ppm) and pollen extract mother solution (1000ppm) were diluted 20000+100000 times, 2000+10000 times, and 200+1000 times, respectively, to obtain 0.05ppm+0.01ppm, 0.5ppm+0.1ppm, and 5ppm+1ppm of the drug. 200mL of each prepared drug was added to the culture box with seeds, fully mixed with the seeds, and the seeds were completely immersed in the drug solution. The treatment was carried out at room temperature for 24h.

[0085] 3) After the corn seeds were soaked for 24 hours, they were rinsed with clean water and sown into the spare culture matrix according to the experimental design, with 20 seeds sown per pot, that is, 100 corn seeds were sown for each treatment, and the results were investigated after 5 days.

[0086] (4) Detection indicators

[0087] The experimental test indicators include: germination rate, root length, root fresh weight, plant height, and plant fresh weight.

[0088] 2. Results Analysis

[0089] (1) Effect of seed soaking with compound extracts on corn germination rate

[0090] As shown in Table 5, after the seed soaking treatment with the compound extract, the germination rate of corn increased at different concentrations. Under the low-concentration compound treatment (0.05ppm+0.01ppm), the germination rate of corn reached 71.0%, and the growth rate reached 20.34%, indicating that the low-concentration compound of tea and pollen extracts can promote the improvement of corn germination rate.

[0091] Table 5 Effects of different concentrations of compound extracts on corn germination rate

[0092]

[0093] (2) Effects of seed soaking with compound extracts on the growth of corn seedlings

[0094] Table 6 shows the effects of the combined extract soaking treatment on the root system and aboveground growth of corn. The results show that after the combined treatment, the longest corn root growth reached 15.2 cm, the root hairs were well developed and strong, and the maximum growth rate of root fresh weight reached 28.57%; the aboveground plant height and fresh weight also increased significantly, with the highest growth rates reaching 16.46% and 34.29% respectively; among them, under the low concentration compound treatment (0.05ppm+0.01ppm), each index has shown a significant promoting effect.

[0095] Overall, after the seed soaking treatment with tea extract and pollen extract, the germination rate and seedling growth of corn were better, especially when the compound treatment was at a concentration where the promoting effect of single-agent treatment was not obvious, it also showed a good effect of promoting germination and growth, indicating that the compound treatment of tea and pollen extracts can synergistically promote the rapid germination and growth of corn.

[0096] Table 6 Effects of different concentrations of compound extracts on seed soaking on the root system and aboveground growth of corn

[0097]

[0098] Test Example 3

[0099] Based on Experimental Example 2, this experimental example verifies the effect of leaf spraying with different concentrations of the compound extract on the growth of pakchoi by pot culture.

[0100] 1. Test methods

[0101] (1) Experimental samples

[0102] Tea extract and pollen extract were prepared with pure water into 0.1% mother solution (i.e., 1000 ppm mother solution), filtered and sterilized, and then diluted and applied according to the experimental design.

[0103] (2) Experimental design

[0104] There were 3 treatments in total, each with 8 replicates, see Table 7 for details.

[0105] Table 7 Experimental design

[0106]

[0107] (3) Experimental procedures

[0108] 1) Culture matrix preparation

[0109] Weigh vermiculite and coconut bran sterilized with high-pressure steam at 121°C for 20 minutes, mix them evenly in a ratio of 2:1, and place them in a pot for later use.

[0110] 2) After the Chinese cabbage seeds were soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they were washed with water for 5-6 times and then sown; when the Chinese cabbage seedlings grew to three leaves and one heart, seedlings of uniform size and free of pests and diseases were selected for transplanting and experimental treatment.

[0111] 3) Preparation of medicine

[0112] According to the experimental design, the prepared tea extract mother liquor and pollen extract mother liquor were diluted 2000+10000 times and 200+1000 times respectively to obtain 0.5ppm+0.1ppm and 5ppm+1ppm agents.

[0113] 4) Experimental treatment

[0114] There was one Chinese cabbage seedling per pot, and each treatment was replicated 8 times, with a total of 24 pots in the experiment. Three days after transplanting, each treatment was sprayed with the prepared agent according to the experimental design until the leaves of the Chinese cabbage were evenly covered with the liquid, but the liquid did not drip down. The control was sprayed with water. The indicators were investigated 7 days after the treatment.

[0115] (4) Detection indicators

[0116] The experimental test indicators include: chlorophyll, leaf area, aboveground fresh weight, and aboveground dry weight.

[0117] 2. Results Analysis

[0118] (1) Effect of compound extract treatment on SPAD value of Chinese cabbage

[0119] Compared with the clear water treatment, after the application of the compound extract, the SPAD value of Chinese cabbage increased in different concentration treatments, mainly in the medium concentration treatment, with the highest growth rate reaching 36.86%.

[0120] Table 8 Effect of compound extract treatment on SPAD value of Chinese cabbage

[0121]

[0122]

[0123] (2) Effects of compound extract treatment on the aboveground biomass of Brassica pekinensis

[0124] As shown in Table 9, the change trends of the fresh weight and dry weight of the aboveground part of pakchoy were consistent, both showing different increases in the fresh weight and dry weight of pakchoy after treatment with the compound extracts. This was mainly reflected in the medium concentration treatment, with the fresh weight and dry weight growth rates reaching as high as 15.34% and 18.63%, respectively.

[0125] Table 9 Effects of compound extract treatment on the aboveground biomass of Brassica pekinensis

[0126]

[0127] (3) Effects of compound extract treatment on changes in leaf area of ​​Chinese cabbage

[0128] Consistent with the biomass change trend of Chinese cabbage, the leaf area of ​​Chinese cabbage increased significantly under the treatment of compound extracts compared with that of pure water, and the leaf area of ​​Chinese cabbage reached 64.87 cm in the medium concentration treatment. 2 , increased by 26.92%, and increased by 20.29% under high concentration treatment.

[0129] Table 10 Effect of compound extract treatment on the change of leaf area of ​​Chinese cabbage

[0130]

[0131] Overall, the combination of tea extract and pollen extract has a significant effect on promoting the growth of Chinese cabbage. When the combination concentration is medium, the growth of Chinese cabbage leaves is better and the biomass is larger.

[0132] Test Example 4

[0133] This test example uses potted culture to test the effect of leaf spraying of tea extract and pollen extract on cucumber growth under low temperature conditions.

[0134] 1. Test methods

[0135] (1) Experimental samples

[0136] Tea extract and pollen extract were prepared with pure water into 1% mother solution (i.e., 10000 ppm mother solution), filtered and sterilized, and then diluted and applied according to the experimental design.

[0137] (2) Experimental design

[0138] There were 7 treatments in total, each with 8 replicates, see Table 11 for details.

[0139] Table 11 Experimental design

[0140]

[0141] (3) Experimental procedures

[0142] 1) Culture matrix preparation

[0143] Vermiculite and coconut bran sterilized with high-pressure steam at 121℃ for 20 minutes are mixed in a ratio of 2:1 and placed in a pot for later use.

[0144] 1) After the cucumber seeds are soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they are washed with water for 5-6 times and then sown; when the cucumber seedlings grow to the point where the first true leaf is fully unfolded, seedlings of uniform size and free of pests and diseases are selected for later use.

[0145] 3) Preparation of medicine

[0146] The tea extract and the pollen extract are first prepared into a 1% mother liquor (i.e., a 10,000 ppm mother liquor), filtered and sterilized, and the prepared tea extract mother liquor is diluted 2,000 times and 200 times, respectively, to obtain 5 ppm and 50 ppm of the drug, and the pollen extract is diluted 10,000 times and 1,000 times, respectively, to obtain 1 ppm and 10 ppm of the drug; the prepared tea extract mother liquor and pollen extract mother liquor are diluted 20,000+100,000 times and 2,000+10,000 times, respectively, to obtain 0.5 ppm+0.1 ppm and 5 ppm+1 ppm of the drug.

[0147] 4) Experimental treatment

[0148] Select the cucumber seedlings of the same size, free of pests and diseases and transplanted them into pots, with one plant per pot and eight replicates per treatment, for a total of 56 pots. According to the experimental design, each treatment was sprayed with the prepared agent until the leaves of the pakchoi were evenly covered with the liquid, but the liquid did not drip down. The control was sprayed with water. After 24 hours of spraying, the seedlings were placed in a low temperature environment of 4℃ for 24 hours, and then the wilting of the cucumber was observed and the water loss rate was measured.

[0149] (4) Detection indicators

[0150] The experimental detection index is the water loss rate of cucumber leaves.

[0151] The first true leaf of the cucumber after low temperature treatment was removed and weighed, and the leaf weight A was recorded. Then, the leaf was placed in clean water for 12 hours to allow the leaf to fully absorb water to saturation, and the leaf weight after water absorption B was recorded.

[0152] Leaf water loss rate (%) = (BA) / B*100

[0153] 2. Results Analysis

[0154] After low temperature (4℃) treatment, it can be observed that the cucumber leaves in the water treatment group are severely wilted, while the cucumber leaves treated with single extract and compound extract have a lower degree of wilting, without obvious curling and softening. The anti-low temperature effect is quantitatively calculated by the water loss rate of cucumber leaves under low temperature conditions. The smaller the water loss rate of the leaves, the better the anti-low temperature effect.

[0155] The water loss rate of cucumber leaves was measured, and it was found that after the treatment with tea extract and pollen extract alone, the water loss rate of cucumber leaves reached 31.16% and 30.47% at medium and high concentrations respectively. After the two extracts were mixed, the water loss rate of cucumber leaves reached 26.20% at medium concentration. It can be seen that the mixed treatment significantly alleviated the impact of low temperature frost damage on the growth of cucumber leaves, which was mainly reflected in the medium concentration treatment.

[0156] Table 12 Effects of different extracts on water loss rate of cucumber leaves under low temperature conditions

[0157]

[0158] Test Example 5

[0159] It can be seen from Experimental Examples 1-4 that after the tea extract and the pollen extract are compounded, they can show obvious synergistic effects at ultra-low concentrations, and can also better alleviate the damage of low temperature to cucumber crops. It can be seen that the activity of the extract after compounding is significantly improved. Therefore, a co-extraction test of tea and pollen was carried out to prepare a tea-pollen composite extract.

[0160] 1. Test methods

[0161] Weigh 1:5 times the amount of tea powder and rape pollen, add water to make the volume 10 times, ultrasonicate at 55°C hot water at an ultrasonic frequency of 20kHz for 30 minutes, repeat the above operation twice, combine the filtrate, then filter, add chitosan to the filtrate, maintain at 60-80°C for at least 1 hour, cool and stand, separate the solid and liquid, and concentrate to 1 / 4 of the volume of the filtrate to obtain a tea-pollen composite extract.

[0162] 2. Test results

[0163] The test results of tea-pollen composite extract are shown in Table 13.

[0164] Table 13 Test results of tea-pollen composite extract

[0165]

[0166]

[0167] Test Example 6

[0168] The effects of tea-pollen composite extract treatment on the growth of pepper crops were tested.

[0169] 1. Test methods

[0170] (1) Experimental sample preparation

[0171] The tea-pollen composite extract in Test Example 5 was prepared into a 0.1% (ie, 1000 ppm) mother solution, filtered and sterilized, and then diluted and applied according to the experimental design.

[0172] (2) Experimental design

[0173] Table 14 Experimental design

[0174]

[0175] (3) Experimental steps:

[0176] 1) Culture matrix preparation

[0177] Vermiculite and coconut bran sterilized with high-pressure steam at 121℃ for 20 minutes are mixed in a ratio of 2:1 and placed in a pot for later use.

[0178] 2) After the pepper seeds are soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they are washed with water 5-6 times and then sown; when the pepper seedlings grow to 4-5 leaves, seedlings of uniform size and free of pests and diseases are selected for planting.

[0179] 3) Preparation of medicine

[0180] The mother solution of the composite extract was diluted 1000 times and 10000 times to obtain 1 ppm and 0.1 ppm of the agent, and then the 1 ppm of the agent was diluted 100 times and 1000 times to obtain 0.01 ppm and 0.001 ppm of the agent.

[0181] 4) Experimental treatment

[0182] Pepper seedlings of uniform size and free of pests and diseases were selected and transplanted into pots, with one plant per pot and eight replicates per treatment, for a total of 40 pots. After 3 days of seedling acclimatization, the prepared agent was sprayed on each treatment according to the experimental design until the pepper leaves were evenly covered with the liquid, but the liquid did not drip down. The control was sprayed with water. The indicators were investigated 7 days after the treatment.

[0183] (4) Detection indicators

[0184] The experimental test indicators include: chlorophyll, fresh weight of aboveground parts, and dry weight of aboveground parts.

[0185] 2. Results Analysis

[0186] (1) Effect of tea-pollen composite extract treatment on SPAD value of pepper

[0187] Table 15 Effects of different concentrations of tea-pollen composite extract on SPAD values ​​of pepper

[0188]

[0189] Compared with pure water, after foliar spraying of different concentrations of compound extract, the growth condition of pepper leaves was significantly better, the pepper leaves were dark green, and the SPAD value growth rate ranged from 8.85% to 17.04%. The SPAD values ​​of the compound extract treatment groups at different concentrations were significantly better than those of the pure water treatment.

[0190] (2) Effect of tea-pollen composite extract treatment on pepper biomass

[0191] As shown in Table 16, after treatment with different concentrations of the composite extract, the fresh weight of the aboveground part of pepper increased significantly, with a growth rate of 8.00%-16.00%, among which the performance was better at 0.1ppm; consistent with the trend of fresh weight changes, the dry weight of the aboveground part of pepper increased by 12.87%-30.69% compared with clear water.

[0192] Table 16 Effects of tea-pollen compound extract treatment on the aboveground biomass of pepper

[0193]

[0194] In summary, the tea-pollen composite extract can show a significant growth-promoting effect even at a lower concentration. At 0.1ppm, the pepper biomass growth rate can reach 16%.

[0195] Test Example 7

[0196] The tea-pollen compound extract was sprayed on wheat seedlings under drought conditions to test the drought resistance of wheat.

[0197] 1. Test methods

[0198] (1) Experimental sample preparation

[0199] The composite extract was prepared into a 0.1% (i.e. 1000 ppm) mother solution, filtered and sterilized, and then diluted and applied according to the experimental design.

[0200] (2) Experimental design

[0201] Table 17 Experimental design

[0202]

[0203]

[0204] (3) Experimental steps:

[0205] 1) Culture matrix preparation

[0206] Weigh a certain weight of vermiculite, add 2 times the volume of tap water (i.e., add 2000mL of tap water to 1kg of vermiculite. In this step, the amount of water absorbed varies depending on the time the vermiculite is opened, so the amount of water added needs to be adjusted according to the actual situation). Mix thoroughly to make planting soil. The water holding capacity of the matrix is ​​200%. Quantitatively weigh 170g of planting soil in each small black square pot, smooth the surface, and press to the same height for later use.

[0207] 2) The wheat seeds were disinfected with 5% sodium hypochlorite for 15 minutes, rinsed with clean water for 6 to 7 times to fully wash away the disinfectant, and two filter papers were placed in the germination box. The filter papers were moistened with water, and the disinfected wheat seeds were spread on the filter papers. The lid was closed and placed in a dark environment at 25°C for germination. After 16 hours, the wheat seeds began to turn white.

[0208] 3) Select plump, intact and white seeds for sowing. Plant 12 seeds in the center of each small square pot in a concentrated 4*3 pattern. The planting depth of each seed is about 0.5 cm. Keep the planting depth of each pot as consistent as possible. Gently smooth the soil surface after planting so that the seeds are completely covered. Place them in a 27℃ culture room for cultivation. Set the light intensity to 10,000 Lux and the lighting time to 14 hours of light and 10 hours of darkness.

[0209] 4) After 5 days, the wheat seedlings in each treatment grew to about 10 cm. Seedlings with uniform growth were selected, and each pot was weighed on a balance. Water was added until the weight of each pot was consistent. At this time, the weight of each pot was about 90 g (the weight of the pot was 12 g, the weight of the soil was 78 g, and the weight of the wheat seedlings was negligible), and the water holding capacity of the substrate was about 50%.

[0210] 5) Preparation of medicine

[0211] The composite extract is first prepared into a 0.1% solution, filtered and sterilized, and diluted 1000 times and 10,000 times to obtain 1 ppm and 0.1 ppm agents. Subsequently, the 1 ppm agent is diluted 100 times and 1000 times to obtain 0.01 ppm and 0.001 ppm agents.

[0212] 6) Spray the wheat seedlings on the leaves, control the number of spraying times for each pot of wheat to ensure the same spraying amount for each treatment, set no less than 6 replicates for each treatment, place them in a 27°C culture room after spraying, set the light intensity to 10,000 Lux, and set the light time to 14 hours of light and 10 hours of darkness. Do not water during this period and wait for natural drought.

[0213] (4) Survey indicators

[0214] After about 3 days of cultivation, drought phenotypes began to appear in each treatment group, manifested as water loss in leaves and lodging of some seedlings. At this time, the weight of each pot was about 70g, and the water holding capacity of the matrix was below 5%. Photos were taken to record the phenotypic differences of each treatment group. The drought treatment was continued for 2 to 3 days until all the seedlings in each treatment group lodged. Sufficient water was added (the weight of each pot was about 190g) for recovery. After 24 hours, photos were taken to record the phenotypes, and the number of surviving seedlings in each treatment group was counted to calculate the survival rate.

[0215]

[0216] 2. Results Analysis

[0217] It can be seen from Table 18 that under drought conditions (substrate water holding capacity <5%), the wheat survival rate in the clean water treatment group was only 16.6%, while after treatment with different concentrations of tea-pollen composite extract, the wheat survival rate reached more than 56%, with the highest reaching 64.9%. This shows that the composite extract treatment can alleviate the impact of drought conditions on wheat, promote wheat recovery after rehydration, and improve wheat's stress resistance.

[0218] Table 18 Effects of different concentrations of compound extracts on wheat survival rate under drought conditions

[0219]

[0220] Test Example 8

[0221] The effect of root irrigation with tea-pollen compound extract on the growth of tomato seedlings was tested.

[0222] 1. Test methods

[0223] (1) Experimental sample preparation

[0224] The composite extract was prepared into a 0.1% (i.e. 1000 ppm) mother solution, filtered and sterilized, and then diluted and applied according to the experimental design.

[0225] (2) Experimental design

[0226] Table 19 Experimental design

[0227]

[0228] (3) Experimental steps:

[0229] 1) Culture matrix preparation

[0230] Vermiculite and coconut bran sterilized with high-pressure steam at 121℃ for 20 minutes are mixed in a ratio of 2:1 and placed in a pot for later use.

[0231] 2) Soak tomato seeds in 5% sodium hypochlorite solution for 10 minutes for disinfection, then wash with water 5-6 times before sowing; when the tomato seedlings grow to 4-5 leaves, select seedlings of uniform size and free of pests and diseases for planting.

[0232] 3) Preparation of medicine

[0233] The composite extract is first prepared into a 0.1% solution, filtered and sterilized, and diluted 1000 times and 10,000 times to obtain 1 ppm and 0.1 ppm agents. Subsequently, the 1 ppm agent is diluted 100 times and 1000 times to obtain 0.01 ppm and 0.001 ppm agents.

[0234] 4) Experimental treatment

[0235] Tomato seedlings of uniform size and free of pests and diseases were selected and transplanted into pots, with one plant per pot and eight replicates per treatment, for a total of 40 pots. After 3 days of seedling acclimatization, the prepared drug was irrigated into the roots of each treatment according to the experimental design, with 100 mL of drug solution per pot, and the control was irrigated with clear water. Indicators were investigated 7 days after treatment.

[0236] (5) Testing indicators

[0237] The experimental test indicators include: chlorophyll, plant height, and fresh weight of aboveground parts.

[0238] 2. Results Analysis

[0239] (1) Effect of root irrigation with tea-pollen compound extract on SPAD value of tomato leaves

[0240] Compared with pure water, after foliar spraying of different concentrations of the compound extract, the growth condition of tomato leaves was significantly better, the leaf color was dark green, and the SPAD value growth rate ranged from 17.57% to 28.12%. The best effect was achieved when the compound extract was 1ppm.

[0241] Table 20 Effects of different concentrations of compound extracts on SPAD values ​​of tomatoes

[0242]

[0243] (2) Effects of different extract treatments on tomato plant height and fresh weight

[0244] As can be seen from Table 21, after treatment with different concentrations of tea-pollen composite extract, the height of tomato plants increased significantly compared with the control, with a growth rate of 18.35%-36.41%, among which the performance was better at 0.01 and 1ppm; the aboveground part of tomatoes increased significantly by 16.77%-25.22% compared with the control, and the performance was better at 0.1-1ppm.

[0245] Table 21 Effects of different extract treatments on the aboveground biomass of pepper

[0246]

[0247] In summary, after the composite extraction of tea and pollen, a significant growth-promoting effect can be shown at a lower concentration. At 0.1-1ppm, the changes in tomato leaves, plant height and fresh weight are more obvious, and the performance effect is better.

[0248] Test Example 9

[0249] The tea-pollen composite extract is combined with agricultural adjuvants such as thickeners, dispersants or emulsifiers to form an agricultural preparation.

[0250] 1. Trial production of tea-pollen compound extract preparation

[0251] The formulation includes: tea-pollen compound extract, thickener-xanthan gum, dispersing emulsifier-alkyl glycoside, defoaming agent and soft water, which are compounded and trial-produced according to different proportions. The specific formula design table is as follows:

[0252] Table 22 Tea-pollen compound extract preparation formula

[0253]

[0254] 2. Investigation on stability of trial formulation

[0255] After trial production of each formulation, the samples were tested for stability storage at high temperature (54°C) and low temperature (0°C). The stability of the formulations was investigated after the samples were stored in hot and cold conditions for 2 weeks. The results showed that a small amount of precipitation occurred in formulation 1 under hot storage conditions, the formulation samples of formulation 2 were stable but had poor dispersibility in water, the samples of formulation 3 had more foam during the dissolution process, and the formulation samples of formulation 4 were homogeneous stable liquids with good dispersibility.

[0256] Therefore, formulation 4 is preferred as the trial formulation of tea-pollen composite extract.

[0257] 3. Formulation trial production process

[0258] The compound extract, thickener-xanthan gum, dispersing emulsifier-alkyl glucoside, defoaming agent and soft water were prepared in the ratio of 10:2:2:0.5:85.5. The specific process operation method is as follows:

[0259] ① Weigh 2% xanthan gum and 85.5% soft water and shear until the xanthan gum is completely dissolved and dispersed to form a uniform liquid for later use;

[0260] ② The liquid in ① is stirred at 55° C., and 10% of the composite extract is accurately weighed and added into a stirring kettle, and stirred at 55° C. for 30 minutes until the liquid is homogeneous;

[0261] ③ Weigh 2% of alkyl polyglycoside and add it to the above ② homogeneous liquid, stir for 3 minutes; then add 0.5% of defoaming agent and stir for 3 minutes to complete the trial production of the formula sample.

[0262] 4. Testing of tea-pollen compound extract preparation indicators

[0263] The basic physicochemical properties and technical indicators of the tea-pollen composite extract preparation are shown in Table 23.

[0264] Table 23 Preparation test results

[0265]

[0266] Test Example 10

[0267] Field bioassay of tea-pollen composite extract preparation: Effects of leaf spraying on leaf growth, fresh weight changes and nutritional quality of field-grown Shanghai green crops.

[0268] 1. Test methods

[0269] (1) Experimental sample preparation

[0270] The tea-pollen composite extract preparation prepared as described in Experimental Example 9 was diluted to a 0.1% (ie, 1000 ppm) mother liquor for later use.

[0271] (2) Experimental design

[0272] Table 24 Experimental design

[0273]

[0274] (3) Experimental treatment

[0275] Shanghai green brocade cultivated in open field was selected as the object. The compound extract preparation was sprayed according to the experimental design at the seedling stage (three leaves and one heart) and the rapid growth stage (6-7 leaves). The changes in leaf color, plant height and fresh weight of Shanghai green brocade were investigated at harvest, and samples were taken for determination of protein and vitamin C nutritional quality.

[0276] 2. Results Analysis

[0277] Table 25 Effect of spraying tea-pollen compound extract preparation on the growth of Shanghai green

[0278]

[0279] As shown in Table 25, after foliar spraying of the composite extract preparation twice, the leaves of Shanghai green vegetables turned green compared with the control, the SPAD value increased by 19.27%-27.69%, and the plant height and fresh weight of Shanghai green increased by 16.42%-29.74%, 28.03% and 35.31%, respectively, which were all more obvious when treated with 1ppm.

[0280] Table 26 Effect of spraying compound extract preparation on nutritional quality of Shanghai green

[0281]

[0282] As shown in Table 26, the composite extract preparation treatment has a significant improvement effect on the quality of Bok Choy. Under the 1 ppm treatment, the vitamin C and protein contents increased by 14.83% and 17.93% compared with the control, with a significant promoting effect.

[0283] Test Example 11

[0284] Field testing of tea-pollen composite extract preparation: Effects of leaf spraying on color change, yield and nutritional quality of Kyoho grapes.

[0285] 1. Test methods

[0286] (1) Experimental sample preparation

[0287] The composite extract preparation prepared as described in Experimental Example 9 was diluted to a 0.1% (i.e., 1000 ppm) mother solution for later use.

[0288] (2) Experimental design

[0289] Table 27 Experimental design

[0290]

[0291] Each treatment was designed with three replicate plots, each with six grape trees, for a total of nine treatment plots.

[0292] (3) Experimental treatment

[0293] According to the experimental design, tea-pollen composite extract preparation was sprayed on Kyoho grapes during the fruit setting, swelling and color change stages. When harvesting, the color changes and theoretical yield of the grapes were investigated, and samples were taken for quality testing.

[0294] 2. Results Analysis

[0295] Table 28 Kyoho grape color changes and theoretical yield changes

[0296]

[0297] Note: The color index is the standard for evaluating the appearance color of the fruit: <2 is yellow-green, 2≤index<4 is pink, 4≤index<5 is red, 5≤index<6 is dark red, and index ≥6 is blue-black.

[0298] After foliar spraying of the compound extract preparation, the color of Kyoho grapes was darker than that of the control, indicating that the color changed faster and the appearance was better. The theoretical yield growth rate of Kyoho grapes reached 10.05%-10.53%, indicating that the compound extract can directly promote the growth of Kyoho grape fruits.

[0299] Table 29 Changes in nutritional quality of Kyoho grapes

[0300]

[0301] Vitamin C, total sugar, soluble solids, etc. are key indicators for evaluating grape quality. As can be seen from Table 25, after foliar spraying of the compound extract preparation, the above indicators increased to varying degrees, with the highest growth rates at a treatment concentration of 1 ppm, reaching 22.07%, 35.07%, and 15.01%, respectively. This indicates that treatment with the compound extract preparation can increase the nutrient content in grapes and improve the flavor of grapes.

[0302] Test Example 12

[0303] Field testing of tea-pollen composite extract preparations: Effects of leaf spraying combined with plant growth regulators on color change, yield and nutritional quality of citrus (Ehime).

[0304] 1. Test methods

[0305] (1) Experimental sample preparation

[0306] The composite extract preparation prepared as described in Experimental Example 9 was diluted to a 0.1% (i.e., 1000 ppm) mother solution for later use.

[0307] (2) Experimental design

[0308] 0.006% coronatine soluble solution is a new pesticide plant growth regulator that has the function of promoting fruit color change and ripening. A tea-pollen composite extract preparation and coronatine compound test were designed to verify the effect of compound treatment on citrus color change. Three replicate plots were designed for each treatment, with 6 citrus trees in each plot, for a total of 12 treatment plots.

[0309] Table 30 Experimental design

[0310]

[0311] (3) Experimental treatment

[0312] According to the experimental design, coronatine and compound extract preparations were sprayed on Ehime citrus during the fruit setting, swelling and color change periods. The color changes of Ehime citrus fruits were investigated during the color change process. The theoretical yield of Ehime citrus was investigated during harvest, and samples were taken for quality testing.

[0313] 2. Results Analysis

[0314] Table 31 Changes in red-turning rate and theoretical yield of Ehime citrus

[0315]

[0316] Compared with the water control, after the different treatments of foliar spraying of citrus, the color change of Ehime citrus was more complete, and the red rate of all fruits reached more than 85%, while the red rate of citrus treated with water control was only 70.0%. At harvest, the theoretical yield of citrus after foliar spraying of compound extract preparation and compound planting regulator reached 2844.89kg / mu, an increase of 13.13% over the control, and the yield-enhancing effect was obvious.

[0317] Compared with the treatment with 0.006% coronatine plant regulator, the red-turning rate and yield of citrus fruits after compounding with the compound extract preparation were improved to varying degrees, with the highest growth rates reaching 5.88% and 4.80% respectively. This shows that the compound extract preparation compound plant regulator can promote faster color change of citrus fruits, more uniform color change, and faster promotion of citrus fruit growth.

[0318] Table 32 Changes in nutritional quality of Ehime citrus

[0319]

[0320] As can be seen from Table 32, compared with the water control, after the treatment of leaf spraying with different agents, the vitamin C, total sugar and soluble solids of citrus increased to varying degrees, reaching 20.31%, 45.86% and 11.50% respectively. Compared with the use of 0.006% coronatine alone, after the combined treatment, the quality indicators of citrus were improved, with the highest growth rates reaching 8.23%, 15.11% and 4.39% respectively, indicating that the combined treatment of compound extract preparations can increase the content of nutrients in citrus and improve the flavor of citrus.

[0321] Test Example 13

[0322] Field test of tea-pollen composite extract preparation: Effect of foliar spraying with potassium dihydrogen phosphate on rice yield and quality improvement.

[0323] 1. Test methods

[0324] (1) Experimental sample preparation

[0325] The composite extract preparation prepared as described in Experimental Example 9 was diluted to a 0.1% (i.e., 1000 ppm) mother solution for later use.

[0326] (2) Experimental design

[0327] Table 33 Experimental design

[0328]

[0329] Each treatment was set up with 3 replicates, and the size of each replicate plot was 2m*3m, for a total of 12 treatment plots.

[0330] (3) Experimental treatment

[0331] During the flowering and ear stages of rice, pesticides were sprayed according to the experimental design. When harvesting, the thousand-grain weight and plot yield of rice were investigated, and samples were taken for quality testing.

[0332] 2. Results Analysis

[0333] Table 34 Changes in rice thousand-grain weight and plot yield

[0334]

[0335] As shown in Table 34, compared with the water treatment, the thousand-grain weight and yield of rice increased significantly after each treatment, and the highest yield increase was the potassium dihydrogen phosphate compound extract preparation treatment, with a growth rate of 19.48% and 26.37%. Compared with the potassium dihydrogen phosphate treatment, the thousand-grain weight and yield of rice increased by 8.69% and 11.29% after compounding, indicating that compound treatment can better increase rice yield.

[0336] Table 35 Changes in rice quality under different treatments

[0337]

[0338] As shown in Table 35, after the treatment with potassium dihydrogen phosphate compound extract preparation, the quality of rice is significantly better, especially after the treatment with 2g / mu of compound extract preparation, the protein content of rice grains increased by 31.75% compared with the clear water control, and increased by 14.0% compared with the treatment with potassium dihydrogen phosphate alone. Similarly, the starch content increased by 1.23% compared with the treatment with potassium dihydrogen phosphate alone, which shows that the use of compound extract preparations combined with potassium dihydrogen phosphate to spray rice can increase the protein and starch content in rice grains and improve the quality of grains while increasing rice yield.

[0339] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant growth regulating composition, characterized in that: It comprises tea extract and pollen extract; wherein the tea is selected from green tea, the pollen extract is rapeseed pollen extract, and the tea extract and / or pollen extract are water extracts; the concentration ratio of the tea extract and the pollen extract is 0.001-100:0.001-100; or, the mass ratio of the tea and pollen is 1:1-10.

2. The composition according to claim 1, characterized in that When tea leaves and pollen are extracted separately, the concentration ratio of the tea leaves extract to the pollen extract is 0.001-100:0.001-100; The tea leaf extraction method comprises: mixing tea leaves and water at a material-liquid ratio of 1:5 to 15, extracting at 35° C. to 75° C. for 20 min to 50 min to obtain the tea leaf extract; The pollen extraction method comprises: mixing pollen and water at a material-liquid ratio of 1:1-5, and filtering to obtain a pollen extract.

3. The composition according to claim 1, characterized in that When tea leaves and pollen are mixed for extraction, the mass ratio of tea leaves to pollen is 1:1-10; Tea leaves, pollen and water are mixed, wherein the solid-liquid ratio of tea leaves, pollen and water is 1:5-15, and the temperature is 50° C.-100° C. for extraction for 1 h-3 h, and the mixed extract of tea leaves and pollen is obtained by filtering.

4. Use of the composition according to any one of claims 1 to 3 in promoting plant growth.

5. The use according to claim 4, characterized in that: The promoting plant growth includes promoting seed germination, and / or promoting the growth of one or more of roots, stems, leaves, or flowers and fruits.

6. The use according to claim 4, characterized in that: When in use, the composition is made into an agricultural product for use, and the agricultural product also includes auxiliary materials, which are one or more of a thickener, a dispersant, a wetting agent, a binder, an emulsifier, a stabilizer, and a solvent.

7. The use according to claim 6, characterized in that: The agricultural product prepared from the composition is used in combination with foliar fertilizer, water-soluble fertilizer, compound fertilizer and pesticide.

8. The use according to claim 6, characterized in that: The dosage form of the product is powder, granule, aqueous solution, mother liquid or mother powder.

9. The use according to claim 4, characterized in that: When used, the single agent of the composition or the prepared agricultural product is used to treat seeds, spray leaves or irrigate roots.

10. The composition or use according to any one of claims 1 to 9, characterized in that: The plants include tobacco, corn, lettuce, wheat, pepper, cucumber, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear, apple and rice.

11. Use of the plant growth regulating composition according to claim 1 in regulating plant stress resistance.

12. The use according to claim 11, characterized in that: The stress resistance is at least one of salt-alkali resistance, cold resistance, drought resistance, and high temperature resistance.

13. A plant growth promoter, characterized in that: The active ingredient of the invention is the composition described in claim 1.

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