A soothing and repairing composition

Abstract

The invention discloses a soothing and repairing composition, belonging to the field of dressing and makeup products. The extraction raw materials thereof include saffron flower extract, prune fruit oil, purslane extract, peanut seed coat extract, alder leaf extract, and gold nanoparticles. The composition provided by the invention has a significant soothing and calming effect on the skin and can effectively improve cell migration rate, enhance skin cell metabolism, and promote skin cell self-repair.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to a soothing and repairing composition. Background Art

[0002] With the accelerated pace of modern life and the complexity of environmental factors, skin problems such as sensitivity, dryness, and inflammation are becoming increasingly common, and consumers are increasingly demanding skin topical products with soothing and repairing effects. Currently on the market, many soothing and repairing skin topical products use plant extracts as the main active ingredients, such as green tea and aloe vera, which have shown certain effects in soothing the skin and reducing inflammation. However, these traditional formulas are still insufficient in terms of rapid repair, deep nourishment, and long-term effect stability.

[0003] In addition, with the development of nanotechnology, new materials such as gold nanoparticles have begun to be used in the cosmetics field due to their unique physical and chemical properties. Gold nanoparticles have excellent skin permeability and biocompatibility, and can carry and promote active ingredients to penetrate deep into the skin, thereby enhancing the repair effect of the product. However, there are relatively few innovative products on the market that combine gold nanoparticles with plant extracts and are used in the field of soothing and repairing.

[0004] On the other hand, natural plant resources such as alder leaves and peanut seed coats are widely used in traditional Chinese medicine and folk medicine to treat skin inflammation and damage, but their application in modern cosmetics has not been fully developed and utilized. In particular, alder leaves are rich in a variety of bioactive ingredients such as flavonoids and polyphenols, which have significant antioxidant, anti-inflammatory and repair effects, but the specific extraction process and compound application still need further research and optimization.

[0005] In summary, it is particularly important to develop a skin topical product that combines natural plant extracts with nanotechnology and has efficient soothing and repairing functions. The present invention aims to combine high-quality raw materials such as peanut seed coat extract, gold nanoparticles and alder leaf extract through scientific proportions and advanced extraction processes to prepare a skin topical composition with significant soothing and repairing effects, high safety and good stability, so as to meet market demand and fill the gap in the industry. Summary of the invention

[0006] The present invention provides a soothing and repairing composition, which achieves the effects of soothing and repairing the skin by matching natural ingredients extracted from plants with gold nanoparticles to achieve synergistic effects.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a method for preparing an Alnus populus leaf extract:

[0009] S1: Take fresh Alnus alnus leaves, dry them in the sun, and grind them using a micro air flow mill to obtain powder A1;

[0010] S2: A1 was extracted by refluxing with water-saturated ethyl acetate, refluxed for 3 times, filtered, and the filtrates were combined to obtain an extract filtrate A2, and the extract filtrate was freeze-dried to obtain a powder A3;

[0011] In a second aspect, the present invention provides a method for preparing gold nanoparticles:

[0012] T1: In a nitrogen environment, take a clean volumetric flask, add 100mL of deionized water, heat to 60°C, and then add 1g of A3 powder, followed by stirring at a speed of 140r / min. After stirring for 20min, add 1g / L 1mL of chloroauric acid solution dropwise, stirring continuously during the addition process. When the color turns wine red, the preparation is complete. Encapsulate the gold nanocolloid B1 in ampoule tubes, 1mL per tube, and place at room temperature away from light.

[0013] In a third aspect, the present invention provides a soothing and repairing composition with the following raw material ratios:

[0014] 3-5 parts of peanut seed coat extract;

[0015] Gold nanoparticles 0.1-0.3 parts;

[0016] 1-3 parts of Alnus populus leaf extract.

[0017] 1-3 parts of saffron flower extract;

[0018] 2-4 parts of prickle oil;

[0019] 0.5-5 parts of purslane extract.

[0020] Furthermore, the raw material ratio of the composition is:

[0021] 4 parts of peanut seed coat extract;

[0022] 0.2 parts of gold nanoparticles;

[0023] 2 parts of Alnus populus leaf extract.

[0024] 1-3 parts of saffron flower extract;

[0025] 2-4 parts of prickle oil;

[0026] 0.5-5 parts of purslane extract.

[0027] In a fourth aspect, the present invention provides a process for preparing raw materials for a residual skin external preparation, and the specific steps are as follows:

[0028] The present invention comprises 1wt%-3wt% of a soothing and repairing composition, 5wt%-7wt% of sodium hyaluronate, 1wt%-2wt% of betaine, 1wt%-2wt% of sodium lauryl sulfate, and the balance is made up of water.

[0029] V1: Add sodium dodecyl sulfate and betaine into deionized water, heat to 60°C, and stir evenly;

[0030] V2: When the temperature of V1 drops to ≤45℃, add sodium hyaluronate and soothing and repairing composition, stir evenly, seal and store at 5-10℃ in a light-proof place.

[0031] In the present invention:

[0032] Saffron flower extract: Saffron (Crocus sativus L.) belongs to the genus Crocus in the family Iridaceae. Its flowers are rich in crocin, glycosides, safranal, crocin, anthocyanin, carotene, lycopene, riboflavin, thiamine, etc. It has multiple effects such as anticonvulsant, antidepressant, anti-inflammatory, and anti-tumor.

[0033] Prinsepia utilis Royle Oil: Prinsepia utilis Royle is a Prinsepia utilis Royle fruit of the Rosaceae family. Its oil is rich in oleic acid, linoleic acid and fat-soluble vitamins. In addition, it also contains monostearate glyceryl, stearyl alcohol, and various mineral elements such as calcium, sulfur, manganese, potassium, etc. It has the effects of moisturizing and nourishing the skin.

[0034] Purslane Extract: Purslane (Portulaca oleracea L.), Portulaca oleracea L., its extract is rich in flavonoids, alkaloids, polysaccharides, saponins, vitamins, etc., and has moisturizing, anti-inflammatory, antibacterial, anti-allergic, whitening and anti-aging effects.

[0035] Peanut seed coat extract: Peanut (Arachis hypogaea), a member of the genus Arachis in the Leguminosae family, is rich in proanthocyanidins and other polyphenolic compounds as well as trace elements, and has whitening and anti-oxidation effects.

[0036] Alnus fortunei leaf extract: Alnus fortunei leaf, belonging to the genus Alnus fortunei of the family Styracaceae, is rich in lupenone, α-amyrin, β-amyrin, lupenol, taraxetanol, betulinic acid and other related terpenes, acids and flavonoids. Its extract has significant anti-inflammatory, antioxidant and cell activity promoting effects.

[0037] Gold nanoparticles: Gold nanoparticles are also called nanogold. Because their particle size is in the nanometer range, they show many unique effects such as: promoting cell metabolism, activating cells, anti-aging, wrinkle removal, whitening, freckle removal, promoting blood circulation, sterilization, anti-inflammation, cleaning pores, etc., and because gold nanoparticles are easy to modify, they can show different effects in different preparation methods, so they are widely used in the cosmetics industry.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] The present invention uses Alnus populus leaf extract supplemented with peanut seed coat extract and specially prepared gold nanoparticles. Due to the huge surface area of ​​the gold nanoparticles, the gold nanoparticles can adhere to and complex the effective ingredients in various plant extracts during the preparation process and carry them into cells, thereby reducing the oxidative inactivation of effective substances in skin care products on the surface of the skin. In addition, the compounding of Alnus populus leaf extract with peanut seed coat extract, Prinus utilis oil, Portulaca oleracea extract and Crocus saffron flower extract can effectively enhance the efficacy of each other's raw materials in the skin, and the compound composition has a significant synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 Gold nanoparticle B1 with a scale bar of 200 nm.

[0041] Figure 2 Gold nanoparticle B1 at 50 nm scale bar.

[0042] Figure 3 Gold nanoparticle B1 colloid. DETAILED DESCRIPTION

[0043] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0044] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0045] Some raw materials and their sources are as follows:

[0046] Crocus sativus flower extract: purchased from Xi'an Qingshuo Import and Export Trading Co., Ltd.

[0047] Primarina purpurogena oil: Shanyang Lianfeng Biotechnology Co., Ltd.;

[0048] Purslane extract: Shanyang Lianfeng Biotechnology Co., Ltd.;

[0049] Peanut seed coat extract: Shanyang Lianfeng Biotechnology Co., Ltd.;

[0050] Alnus populus leaves: Ningxia Vanilla Biotechnology Co., Ltd.;

[0051] Chloroauric acid: Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0052] Gold nanoparticles (commercially available) (20-40 nm): Shanghai Aladdin Biochemical Technology Co., Ltd.

[0053] Preparation, comprising the following steps:

[0054] The preparation method of Alnus populus leaf extract is as follows:

[0055] S1: Wash fresh Alnus populus leaves, remove impurities, dry them in the sun, crush them, discard the leaf veins, and then grind them using a micro air flow mill to obtain powder A1;

[0056] S2: A1 was soaked in petroleum ether for 8 hours to remove the pigment, and then the residue was filtered out and dried. After that, it was extracted by reflux with water-saturated ethyl acetate at a reflux temperature of 40-50°C for 3 times. The filtrates were combined to obtain the extract filtrate A2. A2 was freeze-dried to obtain powder A3.

[0057] The gold nanoparticle preparation method is as follows:

[0058] T1: In a nitrogen environment, take a clean volumetric flask, add 100mL of deionized water, heat to 60°C, and then add 1g of A3 powder, followed by stirring at a speed of 140r / min. After stirring for 20min, add 1g / L 1mL of chloroauric acid solution dropwise, stirring continuously during the addition process. When the color turns wine red, the preparation is complete. Encapsulate the gold nanocolloid B1 in ampoule tubes, 1mL per tube, and place at room temperature away from light.

[0059] A soothing and repairing composition has a raw material ratio, and the specific weight ratio is shown in Table 1:

[0060] Table 1

[0061] Experimental Group Peanut Seed Coat Extract Gold nanoparticles (B1) Alnus populus leaf extract A3 Crocus Flower Extract Prinus prickle oil Purslane Extract Gold nanoparticles (commercially available) Peanut Extract Gold nanoparticles (B2) Example 1 4 0.2 2 2 3 2.5 0 0 0 Example 2 3 0.3 3 1 2 0.5 0 0 0 Example 3 5 0.1 1 1 4 5 0 0 0 Comparative Example 1 4 0 2 2 3 2.5 0.2 0 0 Comparative Example 2 4 0 2 2 3 2.5 0 0 0.2 Comparative Example 3 4 0 2 2 3 2.5 0 0 0 Comparative Example 4 0 0.2 2 2 3 2.5 0 0 0 Comparative Example 5 4 0.2 0 2 3 2.5 0 0 0 Comparative Example 6 0 0.2 2 2 3 2.5 0 4 0 Comparative Example 7 0 0.2 0 2 3 2.5 0 0 0

[0062] Compared with gold nanoparticles B1, gold nanoparticles B2 are prepared without stirring.

[0063] A raw material ratio of a leave-on skin topical preparation, the specific mass percentage ratio is shown in Table 2:

[0064] Table 2

[0065] Experimental Group Composition Composition No. Sodium Hyaluronate Betaine Sodium Lauryl Sulfate Example 4 1% Example 1 5% 2% 1.5% Example 5 2% Example 1 6% 1.5% 1% Example 6 3% Example 1 7% 1% 2% Example 7 2% Example 2 6% 1.5% 1% Example 8 2% Example 3 6% 1.5% 1% Comparative Example 8 2% Comparative Example 1 6% 1.5% 1% Comparative Example 9 2% Comparative Example 2 6% 1.5% 1% Comparative Example 11 2% Comparative Example 3 6% 1.5% 1% Comparative Example 12 2% Comparative Example 4 6% 1.5% 1% Comparative Example 13 2% Comparative Example 5 6% 1.5% 1% Comparative Example 14 2% Comparative Example 6 6% 1.5% 1% Comparative Example 15 2% Comparative Example 7 6% 1.5% 1% Comparative Example 16 0% none 6% 1.5% 1%

[0066] Note: Make up the balance with deionized water.

[0067] A specific preparation method of a leave-on skin topical preparation is as follows:

[0068] V1: Add sodium dodecyl sulfate and betaine into deionized water, heat to 60°C, and stir evenly;

[0069] V2: When the temperature of V1 drops to ≤45℃, add sodium hyaluronate and soothing and repairing composition, stir evenly, seal and store at 5-10℃ in a light-proof place.

[0070] Safety Test

[0071] With reference to the 2015 Technical Specifications for Safety of Cosmetics, the topical skin preparations of Examples 4-8 were evaluated for cosmetic irritation. The test method was a skin patch test, and 50 randomly distributed people aged 16-65 years were tested.

[0072] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0073] Evaluation criteria:

[0074] Grade 0: negative reaction;

[0075] Grade 1: Suspicious reaction, only slight erythema;

[0076] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0077] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0078] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0079] Test results: Skin reactions were negative in all subjects.

[0080] The above test results show that the skin topical preparation containing the soothing and repairing composition provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0081] Cytotoxicity assay

[0082] Take the human immortalized epidermal cell line (HaCaT) with stable growth status and adjust the cell concentration to 1×10 5 / mL, inoculate 100μL per well into a 96-well cell culture plate (except the outermost well and the blank control well). Add 200μL of PBS to the outermost well of the cell culture plate to reduce evaporation of the culture medium. Place in a carbon dioxide incubator at 37℃±1℃, CO 2 Culture under the condition of concentration of 5.0%±1% until the cell fusion reaches more than 90%. Add samples with a sample concentration of 10 mg / L, set blank control and sample group, 100 μL per well. Start to add samples (sample group) and PBS (blank control) and keep the time interval between each two columns consistent to ensure that the intervention time of each test substance on the cells is 5 min. After 5 min, remove the test substances in turn, add PBS to each well to wash the test substances, and wash twice. When using a vacuum suction pump or a discharge gun to remove the liquid in the well, be careful to avoid sucking away the surviving cells and affecting the experimental results. Add 100 μL of complete culture medium and 10 μL of CCK-8 solution to each well, respectively, and place in an incubator for about 2 hours. At a wavelength of 450 nm, measure the absorbance (OD) value and calculate the cell viability; the results are shown in Table 3.

[0083] Formula (1) ×100%

[0084] Table 3 Cytotoxicity test

[0085] Experimental Group HaCaT survival rate Example 4 98.34±0.73 Example 5 97.27±0.42 Example 6 97.48±0.17 Example 7 97.17±0.07 Example 8 97.52±0.35 blank 100%

[0086] According to the experimental results, the cell survival rate remained above 90%. The combination of the present experiment had little effect on cell viability.

[0087] DLS particle size test

[0088] The stratum corneum is the skin barrier, which effectively blocks foreign substances from penetrating into the deeper tissues, and of course also hinders the effective ingredients in cosmetics from repairing and treating the deep tissue cells of the skin. Using microparticles with a size of less than 600 nanometers can effectively penetrate the stratum corneum and enter the deep cortex.

[0089] Using DLS dynamic light scattering instrument, the target 95% particle size range can be easily and accurately detected. The specific data are shown in Table 4.

[0090] Table 4

[0091] Experimental Group 95% particle size range (nanometers) B1 20-38 Commercially available 20-35 B2 100+

[0092] Compared with B1, stirring was removed during the preparation of B2.

[0093] As can be seen from Table 4, the prepared gold nanoparticles meet the requirements of the present invention, and their particle size range is almost the same as that of commercially available ones.

[0094] Hyaluronidase inhibition assay

[0095] Hyaluronic acid, also known as hyaluronic acid, is a powerful moisturizer. It can attract and lock in moisture, maintain skin moisture, and improve dry, rough and dehydrated skin conditions. Hyaluronic acid can also enhance the biological activity of cells and has a strong synergistic effect on repairing and protecting the skin. Therefore, by inhibiting hyaluronidase, the decomposition rate of hyaluronic acid in the skin is reduced, thereby achieving long-term moisture retention and repair of the skin, which is of great significance.

[0096] (1) Take four groups of clean test tubes and label them A, B, C, and D. Add 50 μL of the sample (10 mg / L) to test tubes A and B, 50 μL of distilled water to test tubes C and D, 50 μL of 500 U / mL hyaluronidase solution to test tubes A and C, and 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37 ℃ incubator for 20 min.

[0097] (2) Add 2.5 mol / L CaCl to each of the four test tubes. 2 The solution was 10 μL and then placed in a 37°C incubator for 20 min.

[0098] (3) Add 50 μL of 0.5 mg / mL sodium hyaluronate solution to test tubes A and C, and add 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37 ℃ incubator for 40 min, then take them out and leave them at room temperature for 10 min.

[0099] (4) Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, and 50 μL of acetylacetone solution to each of the four test tubes. Place the test tubes in a boiling water bath for 30 min, then place them in an ice bath for 10 min, and finally place them at room temperature for 10 min.

[0100] (5) Add 100 μL of P-DAB reagent to each test tube and measure the absorbance at 530 nm using an ELISA reader.

[0101] Formula (2) Hyaluronidase inhibition rate % = ×100%

[0102] Wherein: A is the OD value of the sample solution; B is the OD value of the sample blank; C is the OD value of the control sample solution; D is the OD value of the control sample blank.

[0103] Table 5

[0104] Experimental Group Hyaluronidase inhibition rate Example 4 82.43% Example 5 84.52% Example 6 84.32% Example 7 83.39% Example 8 84.15% Comparative Example 8 47.13% Comparative Example 9 27.32% Comparative Example 11 32.72% Comparative Example 12 33.57% Comparative Example 13 24.43% Comparative Example 14 35.57% Comparative Example 15 6.41% Comparative Example 16 84.73%

[0105] According to the analysis of the results: From the results of Examples 4-8, it can be seen that the composition provided in the embodiments of the present invention has good hyaluronidase inhibitory effect, especially the effect of Example 5 is better, indicating that the process and formula combination defined in the present invention can effectively improve the inhibitory effect of the composition on hyaluronidase.

[0106] Compared with the results of Example 5 and Comparative Examples 8-9, the gold nanoparticles prepared under a special process can effectively improve the inhibitory effect of the composition on hyaluronic acid, and the gold nanoparticles under a special particle size restriction can effectively improve the inhibitory effect of the composition on hyaluronic acid.

[0107] From the results of Example 5 and Comparative Examples 11-14, it can be seen that the composition has a significant synergistic effect.

[0108] Compared with the results of Example 5 and Comparative Examples 15-16, the composition has a significant effect of inhibiting hyaluronidase, but there is no linear relationship between its added amount and its hyaluronidase inhibitory effect.

[0109] ABTS + Free radical inhibition experiment

[0110] Take 7 mmol / L ABTS + 5 mL and 88 μL of 140 mmol / L potassium persulfate were mixed evenly to prepare ABTS + Free radical stock solution, keep away from light for future use. Take 0.1 mL, 10 mg / L test solution of Examples 4-8 and Comparative Examples 8-16, add 3.9 mL ABTS + The working solution was shaken for 30 seconds and allowed to stand at room temperature for 6 minutes. The absorbance was measured at 734 nm with anhydrous ethanol as the control and Vc as the positive control. Each sample was measured 3 times and the scavenging rate of ABTS+ free radicals was calculated according to the following formula:

[0111] Clearance %= ×100%

[0112] Where Ai is the absorbance value of the test solution; Aj is the background value of the test solution; A0 is the control value without adding the test solution.

[0113] Table 6 ABTS + Free radical scavenging rate

[0114] Experimental Group <![CDATA[ABTS + Free radical scavenging rate / %]]> Example 4 73.49 Example 5 78.17 Example 6 72.53 Example 7 75.24 Example 8 74.37 Comparative Example 8 31.24 Comparative Example 9 23.55 Comparative Example 11 27.13 Comparative Example 12 30.24 Comparative Example 13 17.51 Comparative Example 14 20.06 Comparative Example 15 7.33 Comparative Example 16 77.39 Positive Control 100

[0115] According to the analysis of the results: From the results of Examples 4-8, it can be seen that the compositions provided in the embodiments of the present invention have a good scavenging rate for ABTS+ free radicals, especially Example 5 has a better effect, indicating that the process and formula combination defined in the present invention can effectively improve the scavenging rate of the composition for ABTS+ free radicals.

[0116] Compared with the results of Example 5 and Comparative Examples 8-9, the gold nanoparticles prepared under a special process can effectively improve the scavenging rate of the composition for ABTS+ free radicals, and the gold nanoparticles under a special particle size restriction can effectively improve the scavenging rate of the composition for ABTS+ free radicals.

[0117] From the results of Example 5 and Comparative Examples 11-14, it can be seen that the composition has a significant synergistic effect.

[0118] Compared with the results of Example 5 and Comparative Examples 15-16, the composition has a significant scavenging effect on ABTS+ free radicals, but there is no linear relationship between its added amount and its scavenging rate on ABTS+ free radicals.

[0119] Effects of the composition on the migration of Hacat cells through scratch wound

[0120] The degree of cell migration has an important impact on wound healing and skin repair. In the process of wound healing, cell migration is an important step for epidermal cells to cover the wound, so the higher the cell migration rate, the faster the wound heals. At the same time, cell migration is also involved in the repair process after skin damage. If cell migration is impaired, it will lead to skin repair disorders, which in turn cause various skin diseases.

[0121] Cells were seeded into 12-well plates at the appropriate seeding density and incubated at 37°C, CO 2 Incubate overnight in an incubator. When the cell plating rate in the 12-well plate reaches about 80%, scratch the sample group and the solvent control group. After the scratching, wash the cells with PBS to remove dead cells, then add fresh EGF-free keratinocyte culture medium, add 50 μL of 10 mg / L, and take pictures and record using an inverted microscope. 37℃, 5%CO 2 The cells were incubated in the incubator for 96 h, and photographed again for statistical analysis using ImageJ software.

[0122] Table 7 Hacat cell migration rate

[0123] Experimental Group Mobility Blank Group 0 Example 4 41.38±1.14 Example 5 47.22±2.43 Example 6 46.57±4.27 Example 7 45.13±1.52 Example 8 44.72±2.11 Comparative Example 8 15.24±4.21 Comparative Example 9 13.37±0.82 Comparative Example 11 27.15±2.24 Comparative Example 12 28.41±1.17 Comparative Example 13 12.24±1.42 Comparative Example 14 19.57±0.84 Comparative Example 15 1.17±0.72 Comparative Example 16 45.51±1.13

[0124] From the analysis of the results in Table 7: From the results of Examples 4-8, it can be seen that the composition provided by the examples of the present invention has the effect of significantly improving the cell migration rate, especially Example 5 has a better effect.

[0125] Comparing Example 5 with Comparative Examples 8-9, Example 5 has the best effect. The gold nanoparticles prepared under a special process can effectively promote the improvement of cell migration rate, and the gold nanoparticles under a special particle size restriction can effectively promote the improvement of cell migration rate.

[0126] From the results of Example 5 and Comparative Examples 11-14, it can be seen that the composition has a significant synergistic effect.

[0127] Compared with the results of Example 5 and Comparative Examples 15-16, the composition has a significant effect of promoting the migration rate of Hacat cells, but there is no obvious linear relationship between the addition amount and the migration rate.

[0128] Test on the immediate repair effect of skin care gel on human body

[0129] Transepidermal water loss (TEWL) is an important parameter for evaluating skin barrier function. It indicates the loss of water from the deep dermis through evaporation of the epidermis. The higher the TEWL, the more water is lost through the skin and the weaker the skin's moisturizing ability.

[0130] The transepidermal water loss rate is closely related to the hydration of the stratum corneum and is a commonly used indicator reflecting the barrier function of the stratum corneum. In the development of cosmetics, the efficacy of moisturizing cosmetics can be evaluated by testing the TEWL value of skin water loss. In addition, the transepidermal water loss rate can also be used in allergic patch tests, contact dermatitis, physical therapy, burns and new tissue monitoring to promptly detect whether the protective function of the skin has been damaged.

[0131] Therefore, transepidermal water loss rate is an important parameter for assessing skin health and is of great significance to the research, development and evaluation of moisturizing products.

[0132] 70 healthy subjects aged 18-60 years old with no injuries on the inner arm were selected and divided into groups, with 10 subjects in each group, including 5 males and 5 females. A sample group and a blank group (i.e., negative control group: no test sample) were set up, and the test area was randomly distributed 1cm inside the volunteer's arm. 2 Area. The forearms (two areas) of the above-mentioned subjects were torn off with medical tape 20 times to induce damage to the skin barrier function, and then 5 mg / mL histamine was added to model for 10 minutes (obvious erythema appeared). One area was treated with 1g of the test product, and the other area was blank as a negative control area. The transepidermal water loss rate (TEWL) of the forearm skin was collected before tape tearing (T0), before using the product (i.e., immediately after histamine) (T1), and 2 hours after using the product (T2). The difference in skin index changes between the product-treated area and the negative control area before and after using the product was used to evaluate the short-term repair effect of the product on skin barrier damage caused by tape tearing and histamine-induced skin irritation, and the average value was taken. The results are shown in Table 8 below.

[0133] Formula (5) Relative transepidermal water loss rate = ×100%

[0134] Table 8 Relative water loss rate of epidermis

[0135] Experimental Group Relative transepidermal water loss Example 4 -8.97% Example 5 -11.34% Example 6 -7.41% Example 7 -9.24 Example 8 -8.51% Comparative Example 15 -0.07 Comparative Example 16 -11.42

[0136] According to the results in Table 8: From the results of Examples 4-8, the gel prepared by the composition has significant efficacy, and Example 5, i.e., the composition has the best efficacy.

[0137] Example 5 and Comparative Example 16 show that excessive addition of the composition does not enhance the efficacy, and there is no linear relationship between the effect and the addition amount.

[0138] Comparing Example 5 with Comparative Example 15, Example 5 has the best effect, which indicates that the composition can effectively improve the moisturizing and soothing effects of the matrix.

[0139] Human body 30s instant soothing effect test

[0140] Volunteer selection criteria: a) healthy volunteers aged 18 to 60 years old; b) subjects with no skin lesions / dermatitis or other factors that affect the measurement; c) those with sensitive and dry faces; d) those with positive lactic acid stinging test.

[0141] The number of volunteers is guaranteed to be more than 30, and the subjects should mainly engage in indoor activities to avoid long-term exposure to light and high temperature or cold environments.

[0142] 80 volunteers aged 22-55 with sensitive facial skin (lactic acid stinging test score ≥ 3 points on both sides of the nose) and dry East Asian skin were selected. A sample group and a blank group (i.e., a model control group: no sample was applied) were set up, and the test areas were randomly distributed on the left and right faces of the volunteers. After using the essence of the present invention for 30 seconds, the red image was collected by the skin facial image analyzer VISIA 7, and the skin redness a* value was analyzed by Image-Pro Plus (IPP) analysis software to evaluate its moisturizing and soothing effects. The laboratory temperature was 21±1℃; the relative humidity was 50%±10%.

[0143] The experiment adopted the self-before and after control method and the blank group control method. The test cycle was 30s, and the skin image collection times were 2 times, 0s and 30s respectively. Set up a sample group and a blank group (i.e., model control group: no sample was applied), and the test areas were randomly distributed on the left and right faces of the volunteers. Apply the sample at a dosage of 0.25g / half face. Select qualified subjects according to the requirements, and when the volunteers visit, sign the volunteer informed consent form with them, collect skin images and questionnaire data after using the product for 30s. When visiting, after washing the face with the designated cleanser, sit quietly in the test environment for about 20-30 minutes. After the sitting is over, start the test.

[0144] With the help of the facial image analyzer VISIA 7 (Canfield®, USA), which uses 3 different light sources to capture facial images, changes in facial skin condition can be observed intuitively.

[0145] The red image a* value was analyzed using Image-Pro Plus software.

[0146] Skin redness a* value: The a* value represents the redness of the skin. An increase in the a* value indicates an increase in the vascular reactivity of the skin.

[0147] The data were analyzed using SPSS 19.0 data analysis software.

[0148] Table 9 Skin redness a* experimental results

[0149]

[0150] From the analysis of the results in Table 9: comparing Examples 5-8, the results show that the skin redness a* of Examples 5-8 is reduced, and the reduction degree is the highest in Example 5, indicating that the skin external preparation prepared by using the composition of the present invention has a soothing effect.

[0151] Comparative Example 5 and Comparative Example 16 show that too high an addition amount of the composition cannot significantly reduce the average value of skin redness, and the addition amount defined in the present invention can maximize the reduction of the skin redness a* value by the composition.

[0152] Comparative Example 5 and Comparative Example 15 and the blank show that the use of the composition prepared by the present invention can achieve the maximum effect of the composition provided by the present invention on reducing the skin redness a* value.

[0153] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A soothing and repairing composition, characterized in that: Made from the following raw materials: 3-5 parts of peanut seed coat extract; Gold nanoparticles 0.1-0.3 parts; 1-3 parts of Alnus populus leaf extract; 1-3 parts of saffron flower extract; 2-4 parts of prickle fruit oil; 0.5-5 parts of purslane extract; The Alnus populus leaf extraction steps are as follows: S1: Wash fresh Alnus populus leaves, remove impurities, dry them in the sun, crush them, discard the leaf veins, and then grind them using a micro air flow mill to obtain powder A1; S2: A1 was soaked in petroleum ether for 8 hours to remove the pigment, and then the residue was filtered out and dried. After that, it was extracted by reflux with water-saturated ethyl acetate at a reflux temperature of 40-50°C for 3 times. The filtrates were combined to obtain the extract filtrate A2. A2 was freeze-dried to obtain powder A3. The steps of preparing the gold nanoparticles are as follows: T1: In a nitrogen environment, take a clean volumetric flask, add 100 mL of deionized water, heat to 60 ° C, and then add 1 g of A3 powder, followed by stirring at a stirring speed of 140 r / min. After stirring for 20 minutes, add 1 mL of chloroauric acid solution dropwise, stirring continuously during the addition process. When the color turns wine red, the preparation is complete. Encapsulate the gold nanoparticles B1 in ampoule tubes, 1 mL per tube, and place at room temperature away from light; The particle size of the gold nanoparticles is 20-40 nm.

2. The soothing and repairing composition according to claim 1, characterized in that: Made from the following raw materials: 4 parts of peanut seed coat extract; 0.2 parts of gold nanoparticles; 2 parts of Alnus populus leaf extract; 2 parts of saffron flower extract; 3 parts of prickle fruit oil; 2.5 parts of Purslane extract.

3. A leave-on skin external preparation, characterized in that: Comprising the soothing and repairing composition according to claim 1 or 2.

4. The skin external preparation according to claim 3, characterized in that: Contains 1wt%-3wt% of a soothing and repairing composition.

5. The skin external preparation according to claim 4, characterized in that: Also includes sodium hyaluronate, betaine, sodium lauryl sulfate.

6. The skin external preparation according to claim 5, characterized in that: The skin external preparation comprises 5wt%-7wt% of sodium hyaluronate, 1wt%-2wt% of betaine, and 1wt%-2wt% of sodium lauryl sulfate.

Images