An integrated nucleic acid detection cartridge for African swine fever virus

Abstract

The present invention relates to the technical field of nucleic acid detection, and specifically to an integrated nucleic acid detection card box for African swine fever virus, comprising a processing plate body and a sample plate body that are integrally fixed and installed, wherein a sample liquid storage cavity is provided in the sample plate body, and the number of the sample liquid storage cavities is provided in a total of several groups, and is evenly distributed in a linear array, wherein a closed bottom groove is provided in the sample liquid storage cavity near the bottom of the processing plate body, and a bottom groove sealing film is provided at the connecting port between the closed bottom groove and the sample liquid storage cavity; the present invention can realize automatic mixing and detection of multiple groups of samples under a closed structure, and only needs to be pressed twice when in use, and the operation is simple and convenient. Compared with the card box for single-chip detection in the prior art, the present invention can have higher efficiency when large-scale general testing is required in pig farms and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to an integrated nucleic acid detection card box for African swine fever virus. Background Art

[0002] African swine fever is an acute, hemorrhagic, and highly contagious disease caused by the infection of domestic pigs and various wild boars by the African swine fever virus. The African swine fever virus is an important member of the genus African swine fever virus of the family Asfrinidae, and some characteristics of the virus are similar to those of the family Iridoviridae and the family Poxviridae. The nucleic acid detection card is one of the main detection methods for in vitro diagnosis of African swine fever virus, and is a detection method with high accuracy. The nucleic acid detection card box in the prior art, such as a nucleic acid detection card box with a nucleic acid pollution prevention number of CN114717290A, records: The nucleic acid detection card box with a nucleic acid pollution prevention is used to detect a chip with a test sample, and the chip includes a sample addition port, a reaction zone, a sealing film (not shown) and a sealing film (not shown), the sealing film is used to seal the sample addition port, and the sealing film is used to seal the reaction zone. Before use, the test sample is added to the reaction zone from the sample addition port, and then the sample addition port is sealed with a sealing film to form a closed chip. After the chip is inserted into the nucleic acid detection card box that prevents nucleic acid contamination, the chip destruction structure destroys the sealing film on the chip to release the test sample, and the blister release module destroys the liquid storage blister to release the buffer solution. The test sample and buffer solution are mixed and then flow into the test strip to display the test result.

[0003] For example, the above-mentioned document records that the nucleic acid detection card box in the prior art can only hold one biological sample at a time when in use, and can only be tested one by one during the detection process, which is not conducive to large-scale general testing in farms when detecting African swine fever virus, and the efficiency is too low. Summary of the invention

[0004] The purpose of the present invention is to provide an African swine fever virus integrated nucleic acid detection card box to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an African swine fever virus integrated nucleic acid detection card box, comprising a processing plate body and a sample plate body that are fixedly installed in one piece, a sample liquid storage cavity is opened in the sample plate body, the number of the sample liquid storage cavities is set in a total of several groups, and is evenly distributed in a linear array, a closed bottom groove is opened in the sample liquid storage cavity near the bottom position of the processing plate body, a bottom groove sealing film is arranged at the communication port between the closed bottom groove and the sample liquid storage cavity, a sealing matching eaves is arranged above the sample liquid storage cavity near the middle position, a split gate is sealed and interspersed in the sealing matching eaves, when the split gate moves down to the right position, the sample liquid storage cavity can be divided into two parts from the middle, a bridge liquid cavity is opened inside the split gate, a first pressing plate is fixedly arranged on the upper end of the split gate, a mixed liquid cavity is opened in the processing plate body, the closed bottom groove is connected to the mixed liquid cavity, a detection card is arranged in the processing plate body, and the detection card is used to automatically detect the mixed liquid in the mixed liquid cavity.

[0006] A one-way blocking eave is fixedly arranged in the closed bottom groove, a one-way blocking piece is arranged below the one-way blocking eave, and a blocking piece spring is arranged below the one-way blocking piece. The blocking piece spring applies an upward elastic thrust to the one-way blocking piece, so that the one-way blocking piece blocks the one-way blocking eave.

[0007] The processing plate body is provided with a collecting transverse cavity and an output cavity. The collecting transverse cavity is connected to the closed bottom groove, one end of the output cavity is connected to the collecting transverse cavity, and the other end of the output cavity is connected to the mixed liquid cavity.

[0008] The slitting gate is fixedly provided with a puncture fixing piece. When the slitting gate moves downward, the puncture fixing piece can be driven to move downward, so that the puncture fixing piece punctures the bottom groove sealing film.

[0009] A positioning outer frame is fixedly provided on the sample plate body, and a pipeline vertical groove is opened on the surface of the positioning outer frame, and the pipeline vertical groove corresponds to the cutting gate plate one by one. A second pressure plate is provided on the positioning outer frame, and a buffer storage bag is provided below the second pressure plate. The buffer storage bag is filled with buffer, and the buffer storage bag corresponds to the cutting gate plate one by one. A connecting hose is provided on the surface of the buffer storage bag, and the connecting hose passes through the pipeline vertical groove and is connected with the bridge liquid cavity.

[0010] A brittle limiting rod is provided between the first pressing plate and the sealing mating eaves, an upper buckle plate is fixedly provided on the lower surface of the first pressing plate, and a lower buckle plate is fixedly provided on the upper surface of the sample plate body. When the first pressing plate is manually pressed, the brittle limiting rod will break first, and then the upper buckle plate and the lower buckle plate will be buckled and fixed to each other.

[0011] A rotating impeller is rotatably arranged in the mixed liquid chamber, a detection cavity is opened in the processing plate body, one end of the detection cavity is connected with the mixed liquid chamber, and a rubber sealing ring is arranged at the other end of the detection cavity, and the rubber sealing ring is in sealing contact with the surface of the detection card.

[0012] The detection card is provided with a detection liquid hole and a display window, the surface of the processing plate body is provided with an observation slot, through which the display condition of the display window can be observed, and the end of the detection card is provided with a support spring.

[0013] A synchronous connecting frame is fixedly arranged on the detection card, a cylindrical inner cavity is opened in the processing plate body, a pressure discharge cavity is connected between the cylindrical inner cavity and the mixed liquid cavity, a synchronous driving piston is sealingly and slidably arranged in the cylindrical inner cavity, and the synchronous driving piston is fixedly installed with the synchronous connecting frame. When the synchronous driving piston moves, the detection card can be driven to move through the synchronous connecting frame, so that the detection liquid hole is connected with the detection cavity correspondingly.

[0014] A cache outer ring is fixedly arranged on the surface of the processing plate body, a cache rubber membrane is sealed in the cache outer ring, the cache rubber membrane is elastic, and the lower part of the cache outer ring is communicated with the cylindrical inner cavity.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The African swine fever virus integrated nucleic acid detection card box of the present invention can realize automatic mixing and detection of multiple groups of samples in a closed structure. It only needs to be pressed twice when in use, and the operation is simple and convenient. Compared with the single-chip detection card box in the prior art, it can have higher efficiency when large-scale general testing is required in pig farms.

[0017] Through the cooperation of the cutting gate, sample liquid storage chamber and closed bottom tank and other structures, the sample can be automatically retained, so that when the mixed test shows positive, the retained sample liquid can be used for secondary separation and locking. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0019] Figure 2 It is a schematic diagram of the overall structure of the present invention from another angle.

[0020] Figure 3 It is a three-dimensional half-section schematic diagram of the sample liquid storage chamber of the present invention.

[0021] Figure 4 It is a three-dimensional half-section schematic diagram of the mixing liquid chamber of the present invention.

[0022] Figure 5It is a three-dimensional half-section schematic diagram of the mixing liquid chamber of the present invention at a horizontal angle.

[0023] Figure 6 It is a three-dimensional half-section schematic diagram of the detection card of the present invention.

[0024] Figure 7 For the present invention Figure 6 A magnified schematic diagram of area A in the middle.

[0025] Figure 8 It is a three-dimensional half-section schematic diagram of the inner cavity of the cylinder of the present invention.

[0026] Fig. 9 It is a schematic diagram of the structure of parts of the present invention.

[0027] In the figure: 1, processing plate; 2, sample plate; 3, sample liquid storage chamber; 4, closed bottom tank; 5, bottom tank sealing film; 6, sealing matching eaves; 7, cutting gate; 8, bridge liquid chamber; 9, first pressure plate; 10, mixed liquid chamber; 11, detection card; 401, one-way blocking eaves; 402, one-way blocking piece; 403, blocking piece spring; 404, summary horizontal cavity; 405, output cavity; 701, puncture fixing piece; 801, positioning outer frame; 802, pipeline vertical groove; 803, second pressure plate; 8 04. Buffer storage bag; 805. Connecting hose; 901. Brittle limit rod; 902. Upper buckle plate; 903. Lower buckle plate; 101. Rotating impeller; 102. Detection cavity; 103. Rubber sealing ring; 104. Detection liquid hole; 105. Display window; 106. Observation slot; 107. Support spring; 108. Synchronous connecting frame; 109. Cylindrical inner cavity; 110. Pressure relief cavity; 111. Synchronous drive piston; 112. Cache outer ring; 113. Cache rubber membrane. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] See also Figures 1 to 9 The present invention provides a technical solution: an integrated nucleic acid detection card box for African swine fever virus, such as Figure 1 and Figure 2 As shown in FIG. 1 , the processing plate body 1 and the sample plate body 2 are integrally fixedly installed. The sample plate body 2 is provided with a sample liquid storage cavity 3. The number of the sample liquid storage cavities 3 is set in a total of several groups and is evenly distributed in a linear array, as shown in FIG. Figure 3As shown in the figure, the sample liquid storage chamber 3 is provided with a closed bottom groove 4 near the bottom position of the processing plate body 1, and a bottom groove sealing film 5 is provided at the communication port between the closed bottom groove 4 and the sample liquid storage chamber 3. A sealing matching eaves 6 is provided above the sample liquid storage chamber 3 near the middle position, and a slitting gate plate 7 is provided in the sealing matching eaves 6 for sealing and interlacing. The sealing matching eaves 6 and the slitting gate plate 7 are in sliding sealing contact through a rubber pad. When the slitting gate plate 7 is moved down to the right position, the sample liquid storage chamber 3 can be divided into two parts from the middle. The inner wall surface of the sample liquid storage chamber 3 is provided with vertical edges, and the sealing can be enhanced by the cooperation between the vertical edges and the slitting gate plate 7. A bridge liquid cavity 8 is provided inside the slitting gate plate 7, as shown in the figure. Figure 3 As shown in FIG, in the undetected state, the lower end of the bridge liquid chamber 8 is in sealing contact with the sealing mating eave 6 to achieve closure.

[0030] A first pressing plate 9 is fixedly arranged on the upper end of the slitting gate 7. Figure 4 As shown in the figure, a mixed liquid chamber 10 is opened in the processing plate body 1, and the closed bottom tank 4 is connected to the mixed liquid chamber 10. A detection card 11 is arranged in the processing plate body 1, and the detection card 11 is used to automatically detect the mixed liquid in the mixed liquid chamber 10.

[0031] like Figure 3 As shown in the figure, a one-way blocking eave 401 is fixedly arranged in the closed bottom tank 4, a one-way blocking piece 402 is arranged below the one-way blocking eave 401, a blocking piece spring 403 is arranged below the one-way blocking piece 402, and the blocking piece spring 403 applies an upward elastic thrust to the one-way blocking piece 402, so that the one-way blocking piece 402 blocks the one-way blocking eave 401. A summary transverse cavity 404 and an output cavity 405 are provided in the processing plate body 1, the summary transverse cavity 404 is connected to the closed bottom tank 4, one end of the output cavity 405 is connected to the summary transverse cavity 404, and the other end of the output cavity 405 is connected to the mixed liquid cavity 10.

[0032] A puncture fixture 701 is fixedly provided on the slitting gate 7. When the slitting gate 7 moves downward, the puncture fixture 701 can be driven to move downward, so that the puncture fixture 701 punctures the bottom groove sealing film 5.

[0033] A positioning outer frame 801 is fixedly provided on the sample plate body 2, and a pipeline vertical groove 802 is opened on the surface of the positioning outer frame 801, and the pipeline vertical groove 802 corresponds to the cutting gate plate 7 one by one. A second pressure plate 803 is provided in the positioning outer frame 801, and a buffer storage capsule 804 is provided below the second pressure plate 803. The buffer storage capsule 804 is filled with buffer, and the buffer storage capsule 804 corresponds to the cutting gate plate 7 one by one. A connecting hose 805 is provided on the surface of the buffer storage capsule 804, and the connecting hose 805 passes through the pipeline vertical groove 802 and is connected with the bridge liquid cavity 8.

[0034] A brittle limiting rod 901 is provided between the first pressing plate 9 and the sealing mating eave 6. The brittle limiting rod 901 is made of a brittle material and can support and limit the first pressing plate 9 when not in use to prevent the first pressing plate 9 from moving downward. When pressed, the brittle limiting rod 901 can break. An upper buckle plate 902 is fixedly provided on the lower surface of the first pressing plate 9, and a lower buckle plate 903 is fixedly provided on the upper surface of the sample plate body 2. When the first pressing plate 9 is manually pressed, the brittle limiting rod 901 will break first, and then the upper buckle plate 902 and the lower buckle plate 903 will be buckled and fixed to each other.

[0035] A rotating impeller 101 is rotatably arranged in the mixed liquid chamber 10, a detection chamber 102 is opened in the processing plate body 1, one end of the detection chamber 102 is connected to the mixed liquid chamber 10, and a rubber sealing ring 103 is arranged at the other end of the detection chamber 102, and the rubber sealing ring 103 is in sealing contact with the surface of the detection card 11.

[0036] The detection card 11 is provided with a detection liquid hole 104 and a display window 105 , and the surface of the processing plate body 1 is provided with an observation slot 106 , through which the display of the display window 105 can be observed, and a support spring 107 is provided at the end of the detection card 11 .

[0037] A synchronous connecting frame 108 is fixedly arranged on the detection card 11, a cylindrical inner cavity 109 is opened in the processing plate body 1, a pressure relief cavity 110 is connected between the cylindrical inner cavity 109 and the mixed liquid cavity 10, and a synchronous driving piston 111 is sealingly and slidably arranged in the cylindrical inner cavity 109. The synchronous driving piston 111 is fixedly installed with the synchronous connecting frame 108. When the synchronous driving piston 111 moves, the detection card 11 can be driven to move through the synchronous connecting frame 108, so that the detection liquid hole 104 is correspondingly connected with the detection cavity 102.

[0038] A buffer outer ring 112 is fixedly disposed on the surface of the processing plate body 1 , and a buffer rubber membrane 113 is enclosed in the buffer outer ring 112 . The buffer rubber membrane 113 is elastic, and the lower part of the buffer outer ring 112 is connected to the cylindrical inner cavity 109 .

[0039] The African swine fever virus integrated nucleic acid detection card box of the present invention is used as follows Figure 2 and Figure 3 As shown in the figure, multiple groups of samples are respectively added to the sample liquid storage chamber 3, and the opening of the sample liquid storage chamber 3 is closed; in one embodiment, the opening of the sample liquid storage chamber 3 is closed by a sealing film; in a second embodiment, the opening of the sample liquid storage chamber 3 is closed by blocking.

[0040] During the test, it is only necessary to place the nucleic acid test box horizontally so that the length direction of the sample liquid storage chamber 3 is parallel to the horizontal plane, and then press the first pressing plate 9 and the second pressing plate 803 in sequence to complete the test. The whole process is closed to avoid sample aerosol contamination.

[0041] When the first pressing plate 9 is first pressed, Figure 3 As shown in the figure, the pressure causes the brittle stopper rod 901 to break, and as the upper buckle plate 902 and the lower buckle plate 903 are pressed, they are buckled into place, thereby fixing the position of the first pressure plate 9. At the same time, the cutting gate plate 7 will move down and be fixed, and the cutting gate plate 7 will be inserted into the sample liquid storage chamber 3 to divide the interior of the sample liquid storage chamber 3 into two parts, thereby retaining the next part of the sample liquid in the sample liquid storage chamber 3. Figure 3 and Figure 4 As shown in the figure, after the cutting gate plate 7 moves down to the right position, the lower end of the bridge liquid cavity 8 will be converted from a closed state to be connected with the inner cavity of the sample liquid storage cavity 3. And the piercing fixture 701 will pierce the bottom tank sealing film 5, so that the sample liquid storage cavity 3 is connected with the closed bottom tank 4.

[0042] When the second pressing plate 803 is pressed for the second time, the buffer storage capsule 804 is squeezed, and the buffer in the buffer storage capsule 804 enters the bridge liquid chamber 8 through the connecting hose 805, and is sprayed out from the lower end of the bridge liquid chamber 8 to flush and mix the sample liquid inside the sample liquid storage chamber 3. Under the action of pressure, the sample liquid and the buffer push open the one-way plugging piece 402 and enter the summary transverse cavity 404. Multiple groups of sample liquids and buffers then enter the mixed liquid chamber 10 together through the output cavity 405. Figure 5 As shown in FIG. 4 , the injection pressure of the output cavity 405 causes the rotary impeller 101 to rotate, thereby further mixing the multiple groups of sample liquids and buffer solutions.

[0043] like Figure 8 As shown in , the mixed liquid continuously enters the mixed liquid chamber 10, causing the pressure in the mixed liquid chamber 10 to increase. The gas-liquid mixture in the mixed liquid chamber 10 enters the cylindrical inner cavity 109 through the pressure discharge channel 110 for buffering. As the pressure in the cylindrical inner cavity 109 gradually increases, the buffer rubber membrane 113 expands and bulges to increase the buffer space. The pressure continues to increase until the synchronous drive piston 111 is driven. When the synchronous drive piston 111 is pushed under pressure, the detection card 11 is driven to move through the synchronous connection frame 108; Figure 7 As shown in the figure, the synchronous connecting frame 108 drives the detection card 11 to move to the left, so that the detection liquid hole 104 is connected to the detection cavity 102. At this time, the mixed liquid in the mixed liquid cavity 10 is diverted to the detection liquid hole 104 through the detection cavity 102 for detection. The detection result is read by observing the display window 105 through the observation slot 106.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An African swine fever virus integrated nucleic acid detection card box, comprising a processing plate and a sample plate that are fixedly installed in one piece, characterized in that: The sample plate body is provided with a sample liquid storage cavity, the number of which is set to several groups and evenly distributed in a linear array, the sample liquid storage cavity is provided with a closed bottom groove near the bottom of the processing plate body, a bottom groove sealing film is provided at the communication port between the closed bottom groove and the sample liquid storage cavity, a sealing matching eaves is provided above the sample liquid storage cavity near the middle position, a slitting gate is provided in a sealing manner, a bridge liquid cavity is provided inside the slitting gate, a first pressing plate is fixedly provided on the upper end of the slitting gate, a mixed liquid cavity is provided in the processing plate body, The closed bottom trough is communicated with the mixed liquid chamber, and a detection card is arranged in the processing plate body, and the detection card is used to automatically detect the mixed liquid in the mixed liquid chamber; a one-way blocking eaves is fixedly arranged in the closed bottom trough, and a one-way blocking piece is arranged below the one-way blocking eaves, and a blocking piece spring is arranged below the one-way blocking piece, and the blocking piece spring applies an upward elastic thrust to the one-way blocking piece, so that the one-way blocking piece blocks the one-way blocking eaves; a summary transverse cavity and an output cavity are opened in the processing plate body, the summary transverse cavity is communicated with the closed bottom trough, one end of the output cavity is communicated with the summary transverse cavity, and the output cavity is communicated with the summary transverse cavity. The other end of the cavity outlet is connected to the mixed liquid cavity; a puncture fixture is fixedly provided on the slitting gate; a positioning outer frame is fixedly provided on the sample plate body, a pipeline vertical groove is provided on the surface of the positioning outer frame, and the pipeline vertical groove corresponds to the slitting gate one by one; a second pressing plate is provided on the positioning outer frame, and a buffer storage bag is provided below the second pressing plate, and a buffer storage bag is filled with a buffer, and the buffer storage bag corresponds to the slitting gate one by one, and a connecting hose is provided on the surface of the buffer storage bag, and the connecting hose passes through the pipeline vertical groove and is connected to the bridge liquid cavity; the mixed liquid A rotating impeller is rotatably arranged in the cavity, a detection cavity is provided in the processing plate body, one end of the detection cavity is communicated with the mixed liquid cavity, a rubber sealing ring is provided at the other end of the detection cavity, and the rubber sealing ring is in sealing contact with the surface of the detection card; a detection liquid hole and a display window are provided on the detection card; a synchronous connecting frame is fixedly arranged on the detection card, a cylindrical inner cavity is provided in the processing plate body, a pressure discharge cavity is communicated between the cylindrical inner cavity and the mixed liquid cavity, a synchronous driving piston is sealingly and slidably arranged in the cylindrical inner cavity, and the synchronous driving piston is fixedly installed with the synchronous connecting frame; In the untested state, the lower end of the bridge liquid cavity is sealed in contact with the sealing mating eaves to achieve closure; after the first pressure plate is pressed, the cutting gate moves down to its position and is fixed, the cutting gate is inserted into the sample liquid storage cavity, and the interior of the sample liquid storage cavity is divided into two parts from the middle, and the lower end of the bridge liquid cavity is converted from a closed state to be connected to the inner cavity of the sample liquid storage cavity, the puncture fixing piece moves down and punctures the bottom groove sealing film; after the second pressure plate is pressed, the buffer storage capsule is squeezed, and the buffer in the buffer storage capsule enters the bridge liquid cavity through the connecting hose and is discharged from the bottom of the bridge liquid cavity The sample liquid and buffer solution push open the one-way plugging piece under the action of pressure and enter the summary horizontal cavity. Multiple groups of sample liquid and buffer solution enter the mixed liquid cavity through the output cavity together. The injection pressure of the output cavity causes the rotating impeller to rotate, and the pressure in the mixed liquid cavity continues to increase. The gas-liquid mixture in the mixed liquid cavity enters the cylindrical inner cavity through the pressure discharge cavity for buffering. The buffer rubber membrane gradually expands and bulges until the synchronous drive piston is driven. When the synchronous drive piston moves, it drives the detection card to move through the synchronous connecting frame, so that the detection liquid hole is connected to the detection cavity accordingly.

2. The African swine fever virus integrated nucleic acid detection card box according to claim 1, characterized in that: A brittle limiting rod is provided between the first pressing plate and the sealing mating eaves, an upper buckle plate is fixedly provided on the lower surface of the first pressing plate, and a lower buckle plate is fixedly provided on the upper surface of the sample plate body. When the first pressing plate is manually pressed, the brittle limiting rod will break first, and then the upper buckle plate and the lower buckle plate will be buckled and fixed to each other.

3. The African swine fever virus integrated nucleic acid detection card box according to claim 1, characterized in that: An observation slot is provided on the surface of the processing plate body, through which the display condition of the display window can be observed, and a supporting spring is provided at the end of the detection card.

4. The African swine fever virus integrated nucleic acid detection card box according to claim 1, characterized in that: A cache outer ring is fixedly arranged on the surface of the processing plate body, a cache rubber membrane is sealed in the cache outer ring, the cache rubber membrane is elastic, and the lower part of the cache outer ring is communicated with the cylindrical inner cavity.

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