Soil remediation bacteria culture box and preparation method of soil remediation bacteria agent

Abstract

The present invention relates to the field of soil remediation bacterial strain preparation, and specifically to a soil remediation bacterial strain culture box and a method for preparing a soil remediation bacterial agent. The invention comprises an incubator body, a liquid taking mechanism, a culture environment maintaining mechanism, a raw material adding mechanism and a culture detection mechanism. The incubator body comprises a partition, an incubator and a water purification chamber. The liquid taking mechanism comprises a liquid taking component, a lifting drive component, a first unlocking component and a second unlocking component. The culture environment maintaining mechanism comprises a gas circulation component, a temperature control component, a disinfection component and a stirring component. The gas circulation component is arranged in the upper cavity, the temperature control component and the stirring component are both located in the incubator, and the original bacterial strain adding component is installed on the top of the incubator body. In the process, the present invention does not require the operator to manually open the incubator body and then carry the containing vessel, which reduces the probability of contamination of the inside of the incubator body, and can realize the continuous production process of the bacterial agent, reduce the liquid taking process, and facilitate large-scale promotion.

Description

Technical Field

[0001] The invention relates to the field of soil remediation bacterial strain preparation, and in particular to a soil remediation bacterial strain culture box and a method for preparing a soil remediation bacterial agent. Background Art

[0002] Soil microbial remediation technology is a technology that uses microorganisms to reduce or degrade pollutants in the soil. This technology mainly relies on indigenous microorganisms or artificially domesticated microorganisms with specific functions, and is achieved through the metabolism of the microorganisms themselves under suitable environmental conditions. The soil remediation bacterial agents used in soil microbial remediation technology need to be cultured separately in an incubator to significantly increase the number of strains to achieve the function of improving the remediation efficiency. Since the conditions for strain culture are relatively harsh, stable temperature, oxygen content, nutrients, etc. need to be provided, and the reproduction of foreign bacteria needs to be avoided, the amount of strains cultured in the incubator is usually limited. The existing strain culture box requires the operator to open the box door to carry and place the inside. This operation method not only requires repeated opening and closing of the cabinet door, which increases the probability of foreign bacteria contaminating the strains, but also the liquid collection process is cumbersome and the operation is cumbersome, which is not conducive to large-scale production operations, and cannot achieve continuous and automatic bacterial agent production functions, and cannot adapt to the current increasing actual production needs. Summary of the invention

[0003] Based on this, it is necessary to provide a soil remediation bacteria culture box and a method for preparing a soil remediation bacteria agent in response to existing technical problems.

[0004] In order to solve the problems of the prior art, the technical solution adopted by the present invention is:

[0005] The present invention provides a soil remediation bacteria culture box, including a culture box body, a liquid collection mechanism, a culture environment maintaining mechanism, a raw material adding mechanism and a culture detection mechanism. The culture box body includes a partition, a culture box and a water purification chamber. The culture box body is divided into an upper chamber and a lower chamber by a horizontally arranged partition. The culture box is installed in the upper chamber, and the water purification chamber is installed in the lower chamber. The liquid collection mechanism includes a liquid collection component, a lifting drive component, a first unlocking component and a second unlocking component. The culture environment maintaining mechanism includes a gas circulation component, a temperature control component, a disinfection component and a stirring component. The raw material adding mechanism includes an original bacteria adding component and an automatic liquid replenishing component. The liquid collection component is arranged below the culture box, the lifting drive component is installed on the partition and is transmission-connected to the liquid collection component, the first unlocking component and the second unlocking component are both transmission-connected to the lifting drive component, the first unlocking component and the second unlocking component are both installed in the upper chamber, the gas circulation component is arranged in the upper chamber, the temperature control component and the stirring component are both located in the culture box, the disinfection component and the automatic liquid replenishing component are both arranged in the culture box body, and the original bacteria adding component is installed on the top of the culture box body.

[0006] Preferably, the liquid collection component includes a liquid collection tube, a connecting tube, a liquid discharge tube and a first electrically-controlled valve. A plurality of first concave bottoms are provided at the bottom of the incubator, a rubber sealing pad is provided on the first concave bottom, a plurality of liquid collection tubes are provided and correspond one to one with the plurality of first concave bottoms, the liquid collection tube can be vertically slidably arranged in the incubator body, the end of the liquid collection tube can penetrate the rubber sealing pad and be inserted into the incubator, the lifting drive component is transmission-connected to the liquid collection tube, the plurality of liquid collection tubes are connected to the connecting tube, the other end of the connecting tube is connected to the liquid discharge tube, the first electrically-controlled valve is installed on the liquid discharge tube, and the second unlocking component is transmission-connected to the liquid discharge tube.

[0007] Preferably, the lifting drive assembly includes a first linear drive and a lifting plate, the first linear drive is fixedly mounted on the partition, the lifting plate is horizontally fixedly mounted on the output end of the first linear drive, the liquid collection tube is fixedly connected to the lifting plate, the first unlocking assembly includes a mounting column, a rotating ring, a torsion spring, a driven ring, a driving ring, a connecting block and a first sealing plate, the mounting column is fixedly mounted between the partition and the incubator, the rotating ring is rotatably sleeved on the mounting column, the torsion spring is sleeved on the mounting column, one end of the torsion spring is fixedly connected to the partition, the other end of the torsion spring is fixedly connected to the rotating ring, and the driven ring can rotate Installed on the mounting column, the rotating ring is fixedly connected to the driven ring, the driving ring is in contact with the driven ring, the lifting plate is fixedly connected to the driving ring, the driving ring is provided with a driving inclined surface, the driven ring is provided with a driven inclined surface matching the driving ring, the end of the driven inclined surface of the driven ring is provided with a first vertical surface, the end of the driving inclined surface of the driving ring is provided with a second vertical surface, the connecting block and the first sealing plate are both provided with a plurality of and correspond to a plurality of first concave bottoms one by one, one end of the connecting block is fixedly connected to the rotating ring, the other end of the connecting block is fixedly connected to the first sealing plate, and the first sealing plate can be sealed and fitted with the bottom of the first concave bottom.

[0008] Preferably, the second unlocking assembly includes a first connecting rod, a second sealing plate, a vertical slide rail, a hinge seat, a second connecting rod and a horizontal slide rail. The second sealing plate is vertically slidably arranged on the inner wall of the incubator body. A liquid outlet opening is provided on the outer wall of the incubator body. The second sealing plate can be completely sealed and arranged at the liquid outlet opening. The second sealing plate is slidably connected to the inner wall of the incubator body through the vertical slide rail. One end of the first connecting rod is hinged to the lifting plate, and the other end of the first connecting rod is hinged to the second sealing plate. The hinge seat is horizontally slidably arranged on the partition through the horizontal slide rail. One end of the second connecting rod is hinged to the second sealing plate, and the other end of the second connecting rod is hinged to the hinge seat. The drain pipe is transmission-connected to the hinge seat.

[0009] Preferably, the temperature control component includes a bottom electric heating plate and a side electric heating plate, the bottom electric heating plate is fixedly mounted on the top of the partition, and the side electric heating plate is fixedly mounted on the inner wall of the upper cavity of the incubator body, the disinfection component includes a first ultraviolet lamp and a second ultraviolet lamp, the first ultraviolet lamp is fixedly mounted on the top of the upper cavity of the incubator body, and the second ultraviolet lamp is fixedly mounted on the bottom of the partition, the stirring component includes a stirring shaft, a stirring drive motor and a stirring paddle, the stirring shaft is vertically arranged inside the incubator body, the stirring drive motor is fixedly mounted on the top of the incubator body, the output end of the stirring drive motor is fixedly connected to the top end of the stirring shaft, the stirring paddle is arranged inside the incubator, and the stirring paddle is fixedly connected to the stirring shaft.

[0010] Preferably, the gas circulation component includes a gas circulation box, an air outlet passage and an air inlet passage. The gas circulation box is fixedly mounted on the outer wall of the incubator body. One end of the air outlet passage is connected to the interior of the gas circulation box, and the other end of the air outlet passage is connected to the interior of the upper cavity of the incubator body. The air inlet passage includes a sealing connecting ring, an air inlet pipe and an air outlet hole. The stirring shaft and the stirring paddle are provided with an air inlet passage, and the air outlet hole is arranged on the stirring paddle. The rotatable shaft of the sealing connecting ring is connected to the stirring shaft, and the sealing connecting ring is connected to the air inlet passage. One end of the air inlet pipe is connected to the gas circulation box, and the other end of the air inlet pipe is connected to the sealing connecting ring.

[0011] Preferably, the original strain adding component includes a strain feeding chamber, a feeding connecting pipe and a second electrically controlled valve. The strain feeding chamber is installed on the top of the incubator body, and the feeding connecting pipe is arranged above the incubator. One end of the feeding connecting pipe passes through the top of the incubator body and is fixedly connected to the strain feeding chamber. The second electrically controlled valve is installed on the feeding connecting pipe. An air blowing pipe is also provided inside the strain feeding chamber, and one end of the air blowing pipe is connected to the gas circulation box.

[0012] Preferably, the automatic liquid replenishment component includes a water tank, an upper water pipe and a water pump. The bottom of the clean water chamber is composed of an inclined surface and a second concave bottom. The second concave bottom is provided with a first water outlet hole. The water tank is fixedly mounted on the outer wall of the incubator body. The water outlet end of the water tank is connected with the clean water chamber. One end of the upper water pipe is connected with the interior of the clean water chamber through the first water outlet hole. The other end of the upper water pipe is arranged above the incubator. The water pump is mounted on the inner wall of the incubator body and the water pump is mounted on the upper water pipe.

[0013] Preferably, the culture detection mechanism includes a bottom temperature detection sensor, a water layer temperature detection sensor and a liquid level detection float, the bottom temperature detection sensor is installed at the bottom of the incubator, the water layer temperature detection sensor is installed in the middle of the incubator, and the liquid level detection float is set on the liquid surface of the incubator.

[0014] A method for preparing a soil remediation bacterial agent is also provided, comprising the following steps:

[0015] S1: During the initial operation, the interior of the incubator body is first disinfected by the first ultraviolet lamp and the second ultraviolet lamp. When the initial strains are added, the initial strains in the strain feeding chamber are introduced into the incubator in the incubator body along the feeding connecting pipe, and the opening and closing of the feeding connecting pipe are controlled by the second electrically controlled valve. When the rehydration operation is required, the culture fluid is provided to the interior of the water purification chamber through the water tank, and the culture fluid is irradiated and disinfected by the second ultraviolet lamp inside the water purification chamber. After the disinfection is completed, the water pump is started to pump water from the water purification chamber through the water supply pipe into the incubator to complete the rehydration operation. The inclined surface and the second concave bottom are arranged to facilitate the emptying of the water in the water purification chamber;

[0016] S2: The function of cultivating bacteria is realized by a culture environment maintaining mechanism, which is used to maintain the oxygen content, temperature, mixing degree of bacteria in the culture liquid, etc. in the culture box during the cultivation process;

[0017] S3: After the bacterial strain culture is completed, the liquid collection operation can be realized through the liquid collection mechanism, the lifting drive component drives the first unlocking component and the second unlocking component to move, the first unlocking component opens the liquid outlet end of the incubator, and the second unlocking component opens the outer wall of the incubator body. At the same time, the liquid collection component is inserted into the incubator to discharge the cultured bacterial agent in the incubator to the outside;

[0018] S4: Stop after a certain amount of discharge, leave enough mother liquid for subsequent culture in the incubator, and then reset the liquid collection mechanism. The automatic liquid replenishment component introduces the culture liquid after disinfection in the water purification chamber into the incubator to realize a continuous culture process.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. In the process, the present invention does not require the operator to manually open the incubator body to carry the containing container, which reduces the contact process between the operator and the inside of the incubator body, reduces the probability of contamination of the inside of the incubator body, and can realize the continuous production process of the microbial agent, improves the production efficiency, and reduces the liquid collection process. The whole equipment can realize the automated production function, improves the production income, and is convenient for large-scale promotion.

[0021] 2. When cultivating bacterial strains, stable temperature control is performed through the temperature control component. The side electric heating plate and the bottom electric heating plate can realize the precise heating function of the liquid inside the incubator. The first ultraviolet lamp can disinfect the incubator and the liquid extraction mechanism inside the upper cavity of the incubator body. The second ultraviolet lamp can disinfect the culture liquid inside the water purification chamber. This setting method can ensure that during continuous bacterial strain cultivation operations, the culture liquid added to the incubator is first disinfected by the second ultraviolet lamp in the water purification chamber, and then introduced into the incubator through the automatic liquid replenishment component after disinfection, thereby ensuring the purity of the bacterial strains during continuous operation.

[0022] 3. When adding the initial strains, the initial strains in the strain feeding chamber are introduced into the incubator inside the incubator body along the feeding connecting pipe. The opening and closing of the feeding connecting pipe is controlled by the second electrically controlled valve. In order to ensure that the strains are completely emptied, the inside of the strain feeding chamber is connected to the gas circulation box through the blowing pipe. The blowing pipe introduces gas to blow all the initial strains in the strain feeding chamber and the feeding connecting pipe into the incubator, ensuring the accuracy of the number of initial strains inside the incubator, thereby ensuring the accuracy of the subsequent bacterial agent ratio. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional structural diagram of a soil remediation bacteria culture box;

[0024] Figure 2 It is a schematic diagram of the three-dimensional structure inside the soil remediation bacteria culture box;

[0025] Figure 3 This is a front view of the soil remediation bacteria culture box;

[0026] Figure 4 This is a partial three-dimensional structure diagram of the soil remediation bacteria culture box Figure 1 ;

[0027] Figure 5 This is a side view of the interior of the soil remediation bacteria incubator;

[0028] Figure 6 It is a three-dimensional structural schematic diagram of the liquid extraction mechanism in the soil remediation bacteria culture box;

[0029] Figure 7 It is a schematic diagram of the three-dimensional structure of the second unlocking component in the soil remediation bacteria culture box;

[0030] Figure 8 It is a front view of the liquid taking mechanism in the soil remediation bacteria culture box;

[0031] Fig. 9 This is a partial three-dimensional structure diagram of the soil remediation bacteria culture box Figure 2 ;

[0032] Fig.10 This is a partial three-dimensional structure diagram of the soil remediation bacteria culture box Figure 3 .

[0033] The numbers in the figure are:

[0034] 1. Incubator body; 11. Partition; 12. Incubator; 121. First concave bottom; 122. Rubber sealing pad; 13. Water purification chamber; 131. Inclined surface; 132. Second concave bottom; 133. First water outlet; 2. Liquid taking mechanism; 21. Liquid taking assembly; 211. Liquid taking pipe; 212. Connecting pipe; 213. Liquid discharge pipe; 214. First electric control valve; 22. Lifting drive assembly; 221. First linear drive actuator; 222, lifting plate; 23, first unlocking assembly; 231, mounting column; 232, rotating ring; 233, torsion spring; 234, driven ring; 235, driving ring; 236, connecting block; 237, first sealing plate; 24, second unlocking assembly; 241, first connecting rod; 242, second sealing plate; 243, vertical slide rail; 244, hinge seat; 245, second connecting rod; 246, horizontal slide rail; 3, Cultivation environment maintaining mechanism; 31. Gas circulation component; 311. Gas circulation box; 312. Air outlet passage; 313. Sealing connection ring; 314. Air inlet pipe; 315. Air outlet; 32. Temperature control component; 321. Side electric heating plate; 322. Bottom electric heating plate; 33. Disinfection component; 331. First ultraviolet lamp; 332. Second ultraviolet lamp; 34. Stirring component; 341. Stirring shaft; 342. Stirring drive motor; 343. Stirring paddle; 4. Raw material adding mechanism; 41. Original strain adding component; 411. Strain feeding chamber; 412. Feeding connecting pipe; 413. Second electric control valve; 42. Automatic liquid replenishing component; 421. Water tank; 422. Water supply pipe; 423. Water pump; 5. Cultivation detection mechanism; 51. Bottom temperature detection sensor; 52. Water layer temperature detection sensor; 53. Liquid level detection float. DETAILED DESCRIPTION

[0035] In order to further understand the features, technical means, specific objectives and functions of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0036] like Figure 1-10As shown, the soil remediation bacteria culture box includes a culture box body 1, a liquid collection mechanism 2, a culture environment maintaining mechanism 3, a raw material adding mechanism 4 and a culture detection mechanism 5. The culture box body 1 includes a partition 11, a culture box 12 and a water purification chamber 13. The culture box body 1 is divided into an upper chamber and a lower chamber by a horizontally arranged partition 11. The culture box 12 is installed in the upper chamber, and the water purification chamber 13 is installed in the lower chamber. The liquid collection mechanism 2 includes a liquid collection component 21, a lifting drive component 22, a first unlocking component 23 and a second unlocking component 24. The culture environment maintaining mechanism 3 includes a gas circulation component 31, a temperature control component 32, a disinfection component 33 and a stirring component 34. The raw material adding mechanism 4 and the culture detection mechanism 5 are provided. The adding mechanism 4 includes an original strain adding component 41 and an automatic liquid replenishing component 42, the liquid taking component 21 is arranged below the incubator 12, the lifting drive component 22 is installed on the partition 11 and is connected to the liquid taking component 21, the first unlocking component 23 and the second unlocking component 24 are both connected to the lifting drive component 22, the first unlocking component 23 and the second unlocking component 24 are both installed in the upper cavity, the gas circulation component 31 is arranged in the upper cavity, the temperature control component 32 and the stirring component 34 are both located in the incubator 12, the disinfection component 33 and the automatic liquid replenishing component 42 are both arranged in the incubator body 1, and the original strain adding component 41 is installed on the top of the incubator body 1.

[0037] The present invention can cultivate bacteria through the incubator body 1, and the partition 11 divides the interior of the incubator body 1 into two chambers. The incubator 12 in the upper chamber is used to cultivate bacteria, and the water purification chamber 13 is used to disinfect the water in the automatic liquid replenishment component 42 to ensure the purity of the bacterial solution. The raw material adding mechanism 4 is used to realize the initial feeding function, the original bacteria adding component 41 is used to introduce the original bacteria into the incubator 12, and the automatic liquid replenishment component 42 is used to introduce the culture solution into the incubator 12. The function of cultivating bacteria is realized through the culture environment maintaining mechanism 3. The culture environment maintaining mechanism 3 is used to maintain the oxygen content, temperature, and mixing degree of bacteria in the bacterial solution in the incubator 12 during the cultivation process. The disinfection component 33 is used to provide the disinfection function, and the gas circulation component 31 is used to maintain the stability of the oxygen concentration during the bacterial culture. The stirring component 34 can not only improve the mixing degree, but also ensure that the oxygen is evenly aerated in the incubator 12, and the temperature control component 32 is used to detect the temperature inside the incubator 12. After the strain culture is completed, the liquid collection operation can be realized through the liquid collection mechanism 2, and the lifting drive component 22 drives the first unlocking component 23 and the second unlocking component 24 to move. The first unlocking component 23 opens the liquid outlet end of the incubator 12, and the second unlocking component 24 opens the outer wall of the incubator body 1. At the same time, the liquid collection component 21 is inserted into the incubator 12 to discharge the cultured bacterial agent in the incubator 12. After a certain amount of discharge, it stops, and the liquid collection component 21 is reset after leaving enough mother liquid for subsequent cultivation in the incubator 12. The automatic liquid replenishment component 42 introduces the culture liquid in the water purification chamber 13 that has been sterilized into the incubator 12 to realize the continuous culture process. In this process, the present invention does not require the operator to manually open the incubator body 1 and then carry the containing device, which reduces the contact process between the operator and the inside of the incubator body 1, reduces the probability of contamination of the inside of the incubator body 1, and can realize the continuous production process of the bacterial agent, improve the production efficiency, and reduce the liquid collection process. The entire equipment can realize the automatic production function, improve the production income, and facilitate large-scale promotion.

[0038] The liquid collection component 21 includes a liquid collection tube 211, a connecting tube 212, a liquid discharge tube 213 and a first electrically-controlled valve 214. A plurality of first concave bottoms 121 are provided at the bottom of the incubator 12, each of which is provided with a rubber sealing pad 122. A plurality of liquid collection tubes 211 are provided and correspond to the plurality of first concave bottoms 121 one by one. The liquid collection tube 211 can be vertically slidably arranged in the incubator body 1, and the end of the liquid collection tube 211 can penetrate the rubber sealing pad 122 and be inserted into the incubator 12. The lifting drive component 22 is transmission-connected with the liquid collection tube 211, and the plurality of liquid collection tubes 211 are communicated with the connecting tube 212. The other end of the connecting tube 212 is communicated with the liquid discharge tube 213. The first electrically-controlled valve 214 is installed on the liquid discharge tube 213, and the second unlocking component 24 is transmission-connected with the liquid discharge tube 213.

[0039] When the equipment needs to perform liquid collection operation, the liquid collection tube 211 connected thereto is driven to move upward by the lifting drive assembly 22, and the top of the liquid collection tube 211 penetrates the rubber sealing pad 122 and communicates with the inside of the incubator 12. The bacterial agent inside the incubator 12 flows into the connecting tube 212 through the liquid collection tube 211 and is discharged outward through the discharge tube 213. Since the incubator 12 is at a high place, the entire liquid flow can be driven by gravity. The first electrically controlled valve 214 is used to control the switch of the discharge tube 213, thereby controlling the amount of bacterial agent flowing out, and completing the automatic liquid collection process.

[0040] The lifting drive assembly 22 includes a first linear drive 221 and a lifting plate 222. The first linear drive 221 is fixedly mounted on the partition 11. The lifting plate 222 is horizontally fixedly mounted on the output end of the first linear drive 221. The liquid collection tube 211 is fixedly connected to the lifting plate 222. The first unlocking assembly 23 includes a mounting column 231, a rotating ring 232, a torsion spring 233, a driven ring 234, a driving ring 235, a connecting block 236 and a first sealing plate 237. The mounting column 231 is fixedly mounted between the partition 11 and the incubator 12. The rotating ring 232 is rotatably sleeved on the mounting column 231. The torsion spring 233 is sleeved on the mounting column 231. One end of the torsion spring 233 is fixedly connected to the partition 11, and the other end of the torsion spring 233 is fixedly connected to the rotating ring 232. The driven ring 2 34 can be rotatably installed on the mounting column 231, the rotating ring 232 is fixedly connected to the driven ring 234, the driving ring 235 is in contact with the driven ring 234, the lifting plate 222 is fixedly connected to the driving ring 235, the driving ring 235 is provided with a driving inclined surface, the driven ring 234 is provided with a driven inclined surface matching the driving ring 235, the end of the driven inclined surface of the driven ring 234 is provided with a first vertical surface, the end of the driving inclined surface of the driving ring 235 is provided with a second vertical surface, the connecting block 236 and the first sealing plate 237 are each provided with a plurality of and correspond to a plurality of first concave bottoms 121 one by one, one end of the connecting block 236 is fixedly connected to the rotating ring 232, the other end of the connecting block 236 is fixedly connected to the first sealing plate 237, and the first sealing plate 237 can be sealed and fitted with the bottom of the first concave bottom 121.

[0041] When the lifting drive assembly 22 is working, it drives the first unlocking assembly 23 to release the sealing setting of the first concave bottom 121 at the bottom of the incubator 12, and exposes the rubber sealing pad 122 above the liquid collection tube 211, so as to facilitate the insertion of the liquid collection tube 211. When the lifting plate 222 rises, it drives the driving ring 235 fixedly connected thereto to rise synchronously. The driving ring 235 cooperates with the driven inclined surface on the driven ring 234 through the driving inclined surface, and drives the driven ring 234 to rotate around the axis of the mounting column 231 during the rising process, and then drives the rotating ring 232 fixedly connected thereto to rotate through the driven ring 234. The rotating ring 232 drives the first sealing plate 237 fixedly connected to the connecting block 236 to rotate synchronously through the connecting block 236, so that the first sealing plate 237 and the first concave bottom 121 are separated from the sealing fitting state, thereby realizing the unlocking function of the incubator 12, facilitating the smooth liquid collection operation, and when no liquid is collected, the first sealing plate 237 can be used to seal a plurality of first concave bottoms 121 to prevent liquid leakage in the incubator 12. It is worth mentioning that the purpose of setting up multiple first concave bottoms 121 and liquid collection tubes 211 is to improve the efficiency of liquid collection. In order to prevent the rubber sealing gasket 122 from being unable to restore to a sealed state after the liquid collection tube 211 is inserted and pulled out, the pinhole of the liquid collection tube 211 is smaller, resulting in a smaller flow rate discharged from the liquid collection tube 211. By setting up multiple groups of liquid collection tubes 211, the liquid discharge efficiency can be improved.

[0042] The second unlocking assembly 24 includes a first connecting rod 241, a second sealing plate 242, a vertical slide rail 243, a hinge seat 244, a second connecting rod 245 and a horizontal slide rail 246. The second sealing plate 242 is vertically slidably arranged on the inner wall of the incubator body 1. A liquid outlet opening is arranged on the outer wall of the incubator body 1. The second sealing plate 242 can be completely sealed and arranged at the liquid outlet opening. The second sealing plate 242 is slidably connected to the inner wall of the incubator body 1 through the vertical slide rail 243. One end of the first connecting rod 241 is hinged to the lifting plate 222, and the other end of the first connecting rod 241 is hinged to the second sealing plate 242. The hinge seat 244 is horizontally slidably arranged on the partition 11 through the horizontal slide rail 246. One end of the second connecting rod 245 is hinged to the second sealing plate 242, and the other end of the second connecting rod 245 is hinged to the hinge seat 244. The drain pipe 213 is transmission-connected to the hinge seat 244.

[0043] When the lifting drive assembly 22 is working, the second unlocking assembly 24 is driven to move synchronously. When the lifting plate 222 rises, the first connecting rod 241 hinged thereto is driven to deflect, and the second sealing plate 242 is pulled to slide vertically on the vertical slide rail 243 through the first connecting rod 241. When the vertical slide rail 243 moves, the liquid outlet opening on the incubator body 1 is opened, so that the drain pipe 213 installed therein can be extended therefrom. At the same time, when the second sealing plate 242 is displaced, the second connecting rod 245 is driven to deflect, and the second connecting rod 245 drives the hinge seat 244 to horizontally displace on the horizontal slide rail 246, and then drives the drain pipe 213 connected thereto to extend outward through the hinge seat 244, so as to facilitate the liquid extraction operation.

[0044] The temperature control component 32 includes a bottom electric heating plate 322 and a side electric heating plate 321. The bottom electric heating plate 322 is fixedly installed on the top of the partition 11, and the side electric heating plate 321 is fixedly installed on the inner wall of the upper cavity of the incubator body 1. The disinfection component 33 includes a first ultraviolet lamp 331 and a second ultraviolet lamp 332. The first ultraviolet lamp 331 is fixedly installed on the top of the upper cavity of the incubator body 1, and the second ultraviolet lamp 332 is fixedly installed on the bottom of the partition 11. The stirring component 34 includes a stirring shaft 341, a stirring drive motor 342 and a stirring paddle 343. The stirring shaft 341 is vertically arranged inside the incubator body 1, the stirring drive motor 342 is fixedly installed on the top of the incubator body 1, and the output end of the stirring drive motor 342 is fixedly connected to the top of the stirring shaft 341. The stirring paddle 343 is arranged inside the incubator 12, and the stirring paddle 343 is fixedly connected to the stirring shaft 341.

[0045] When the strains are cultured, the temperature control component 32 is used to perform stable temperature control, the side electric heating plate 321 and the bottom electric heating plate 322 can realize the precise heating function of the liquid inside the incubator 12, the first ultraviolet lamp 331 can sterilize the incubator 12 and the liquid taking mechanism 2 inside the upper cavity of the incubator body 1, and the second ultraviolet lamp 332 can sterilize the culture liquid inside the water purification chamber 13. This setting method can ensure that during the continuous strain culture operation, the culture liquid added to the incubator 12 is first sterilized in the water purification chamber 13 by the second ultraviolet lamp 332, and then introduced into the incubator 12 through the automatic liquid replenishment component 42 after the sterilization is completed, so as to ensure the purity of the strains during continuous operation. When the stirring drive motor 342 is working, it drives the stirring shaft 341 to rotate, and the stirring shaft 341 drives the stirring paddle 343 fixedly connected thereto to rotate synchronously, and the stirring operation of the bacterial liquid inside the incubator 12 is realized by the stirring paddle 343, so as to ensure the uniform mixing of the bacterial liquid.

[0046] The gas circulation component 31 includes a gas circulation box 311, an air outlet passage 312 and an air inlet passage. The gas circulation box 311 is fixedly mounted on the outer wall of the incubator body 1. One end of the air outlet passage 312 is connected to the interior of the gas circulation box 311, and the other end of the air outlet passage 312 is connected to the interior of the upper cavity of the incubator body 1. The air inlet passage includes a sealing connection ring 313, an air inlet pipe 314 and an air outlet hole 315. The stirring shaft 341 and the stirring paddle 343 are provided with an air inlet passage, and the air outlet hole 315 is provided on the stirring paddle 343. The rotatable shaft of the sealing connection ring 313 is connected to the stirring shaft 341, and the sealing connection ring 313 is connected to the air inlet passage. One end of the air inlet pipe 314 is connected to the gas circulation box 311, and the other end of the air inlet pipe 314 is connected to the sealing connection ring 313.

[0047] When gas circulation is performed inside the incubator 12, oxygen is introduced into the sealed connecting ring 313 through the air inlet pipe 314, and is discharged into the air outlet 315 through the air inlet channel. During the discharge process, the stirring paddle 343 rotates continuously, thereby making the aeration process more uniform and ensuring the effect of bacterial culture. The exhaust gas is extracted through the air outlet passage 312 to complete the gas circulation process inside the incubator body 1.

[0048] The original bacterial strain adding component 41 includes a bacterial strain feeding chamber 411, a feeding connecting pipe 412 and a second electrically controlled valve 413. The bacterial strain feeding chamber 411 is installed on the top of the incubator body 1, and the feeding connecting pipe 412 is arranged above the incubator 12. One end of the feeding connecting pipe 412 passes through the top of the incubator body 1 and is fixedly connected to the bacterial strain feeding chamber 411. The second electrically controlled valve 413 is installed on the feeding connecting pipe 412. A blowing pipe is also provided inside the bacterial strain feeding chamber 411, and one end of the blowing pipe is connected to the gas circulation box 311.

[0049] When adding the initial strains, the initial strains in the strain feeding chamber 411 are introduced into the incubator 12 in the incubator body 1 along the feeding connecting pipe 412, and the opening and closing of the feeding connecting pipe 412 are controlled by the second electrically-controlled valve 413. In order to ensure that the strains are completely emptied, the interior of the strain feeding chamber 411 is connected to the gas circulation box 311 through an air blowing pipe. The air blowing pipe introduces gas to blow all the initial strains in the strain feeding chamber 411 and the feeding connecting pipe 412 into the incubator 12, thereby ensuring the accuracy of the number of initial strains in the incubator 12, thereby ensuring the accuracy of the subsequent bacterial agent ratio.

[0050] The automatic liquid replenishment component 42 includes a water tank 421, an upper water pipe 422 and a water pump 423. The bottom of the clean water chamber 13 is composed of an inclined surface 131 and a second concave bottom 132. The second concave bottom 132 is provided with a first water outlet hole 133. The water tank 421 is fixedly installed on the outer wall of the incubator body 1. The water outlet end of the water tank 421 is connected with the clean water chamber 13. One end of the upper water pipe 422 is connected with the inside of the clean water chamber 13 through the first water outlet hole 133. The other end of the upper water pipe 422 is arranged above the incubator 12. The water pump 423 is installed on the inner wall of the incubator body 1, and the water pump 423 is installed on the upper water pipe 422.

[0051] When rehydration is required, culture medium is provided to the inside of the water purification chamber 13 through the water tank 421. The culture medium is irradiated and disinfected by the second ultraviolet lamp 332 inside the water purification chamber 13. After disinfection, the water pump 423 is started to pump water from the water purification chamber 13 through the water supply pipe 422 into the incubator 12 to complete the rehydration operation. The arrangement of the inclined surface 131 and the second concave bottom 132 facilitates the draining of the water in the water purification chamber 13.

[0052] The culture detection mechanism 5 includes a bottom temperature detection sensor 51, a water layer temperature detection sensor 52 and a liquid level detection float 53. The bottom temperature detection sensor 51 is installed at the bottom of the trough of the incubator 12, the water layer temperature detection sensor 52 is installed in the middle of the incubator 12, and the liquid level detection float 53 is set on the liquid surface of the incubator 12.

[0053] The bottom temperature detection sensor 51 can be used to detect the temperature of the liquid at the bottom of the incubator 12, and the water layer temperature detection sensor 52 can be used to detect the temperatures of different water layers. The cooperation of the bottom temperature detection sensor 51 and the water layer temperature detection sensor 52 can better detect the liquid temperature, thereby adjusting the heating temperature of the side electric heating plate 321 and the bottom electric heating plate 322 as needed. The liquid level detection float 53 is used to detect the height of the liquid level, so as to facilitate precise control of the amount of initial liquid addition or subsequent liquid addition.

[0054] The preparation method of the soil remediation bacterial agent comprises the following steps:

[0055] S1: During the initial operation, the first ultraviolet lamp 331 and the second ultraviolet lamp 332 are used to sterilize the inside of the incubator body 1. When the initial strains are added, the initial strains in the strain feeding chamber 411 are introduced into the incubator 12 in the incubator body 1 along the feeding connecting pipe 412. The opening and closing of the feeding connecting pipe 412 is controlled by the second electrically controlled valve 413. When the liquid replenishment operation is required, the culture fluid is provided to the inside of the water purification chamber 13 through the water tank 421. The culture fluid is irradiated and sterilized by the second ultraviolet lamp 332 in the water purification chamber 13. After the sterilization is completed, the water pump 423 is started to pump water from the water purification chamber 13 through the upper water pipe 422 into the incubator 12 to complete the liquid replenishment operation. The inclined surface 131 and the second concave bottom 132 are arranged to facilitate the emptying of the water in the water purification chamber 13.

[0056] S2: The function of cultivating bacteria is realized by the culture environment maintaining mechanism 3, and the culture environment maintaining mechanism 3 is used to maintain the oxygen content, temperature, mixing degree of bacteria in the bacterial solution, etc. in the culture box 12 during the cultivation process;

[0057] S3: After the bacterial strain culture is completed, the liquid collection operation can be realized through the liquid collection mechanism 2, and the lifting drive component 22 drives the first unlocking component 23 and the second unlocking component 24 to move, the first unlocking component 23 opens the liquid outlet end of the incubator 12, and the second unlocking component 24 opens the outer wall of the incubator body 1. At the same time, the liquid collection component 21 is inserted into the incubator 12 to discharge the cultured bacterial agent in the incubator 12 to the outside;

[0058] S4: Stop after a certain amount of discharge, leaving enough mother liquid for subsequent culture in the incubator 12, and then reset the liquid extraction mechanism 2, and the automatic liquid replenishment component 42 introduces the culture liquid after disinfection in the water purification chamber 13 into the incubator 12 to realize a continuous culture process.

[0059] The above embodiments only express one or several implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the attached claims.

Claims

1. A soil remediation bacteria culture box, characterized in that: The invention comprises an incubator body (1), a liquid taking mechanism (2), a culture environment maintaining mechanism (3), a raw material adding mechanism (4) and a culture detection mechanism (5); the incubator body (1) comprises a partition (11), an incubator (12) and a water purification chamber (13); the incubator body (1) is divided into an upper chamber and a lower chamber by a horizontally arranged partition (11); the incubator (12) is installed in the upper chamber, and the water purification chamber (13) is installed in the lower chamber; the liquid taking mechanism (2) comprises a liquid taking component (21), a lifting drive component (22), a first unlocking component (23) and a second unlocking component (24); the culture environment maintaining mechanism (3) comprises a gas circulation component (31), a temperature control component (32), a disinfection component (33) and a stirring component (34); the raw material adding mechanism (4) The invention comprises an original strain adding component (41) and an automatic liquid replenishing component (42), wherein the liquid taking component (21) is arranged below the incubator (12), the lifting drive component (22) is installed on the partition (11) and is in driving connection with the liquid taking component (21), the first unlocking component (23) and the second unlocking component (24) are both in driving connection with the lifting drive component (22), the first unlocking component (23) and the second unlocking component (24) are both installed in the upper chamber, the gas circulation component (31) is arranged in the upper chamber, the temperature control component (32) and the stirring component (34) are both located in the incubator (12), the disinfection component (33) and the automatic liquid replenishing component (42) are both arranged in the incubator body (1), and the original strain adding component (41) is installed on the top of the incubator body (1); The liquid collection assembly (21) comprises a liquid collection tube (211), a connecting tube (212), a liquid discharge tube (213) and a first electrically controlled valve (214); a plurality of first concave bottoms (121) are provided at the bottom of the incubator (12); a rubber sealing pad (122) is provided on the first concave bottoms (121); a plurality of liquid collection tubes (211) are provided and correspond one to one with the plurality of first concave bottoms (121); the liquid collection tubes (211) are arranged in a vertically slidable manner in the incubator body (1); The end of the liquid collection tube (211) can penetrate the rubber sealing pad (122) and be inserted into the interior of the incubator (12); the lifting drive assembly (22) is in driving connection with the liquid collection tube (211); a plurality of liquid collection tubes (211) are in communication with the connecting tube (212); the other end of the connecting tube (212) is in communication with the liquid discharge tube (213); the first electrically controlled valve (214) is mounted on the liquid discharge tube (213); and the second unlocking assembly (24) is in driving connection with the liquid discharge tube (213); The lifting drive assembly (22) comprises a first linear drive (221) and a lifting plate (222), the first linear drive (221) being fixedly mounted on the partition (11), the lifting plate (222) being horizontally fixedly mounted on the output end of the first linear drive (221), the liquid extraction tube (211) being fixedly connected to the lifting plate (222), and the first unlocking assembly (23) comprising a mounting column (231), a rotating ring (232), a torsion spring (233), a driven ring (234), a driving ring (235), a connecting block (236) and a first sealing plate (237), the mounting column (231) is fixedly mounted between the partition (11) and the incubator (12), the rotating ring (232) is rotatably sleeved on the mounting column (231), the torsion spring (233) is sleeved on the mounting column (231), one end of the torsion spring (233) is fixedly connected to the partition (11), and the other end of the torsion spring (233) is fixedly connected to the rotating ring (232). The driven ring (234) is rotatably mounted on the mounting column (231), the rotating ring (232) is fixedly connected to the driven ring (234), the driving ring (235) is abuttingly connected to the driven ring (234), the lifting plate (222) is fixedly connected to the driving ring (235), the driving ring (235) is provided with a driving inclined surface, the driven ring (234) is provided with a driven inclined surface matching the driving ring (235), and the driven inclined surface of the driven ring (234) is provided at the end thereof. A first vertical surface, a second vertical surface is provided at the end of the driving inclined surface of the driving ring (235), a plurality of connecting blocks (236) and a first sealing plate (237) are provided and correspond one to one with the plurality of first concave bottoms (121), one end of the connecting block (236) is fixedly connected to the rotating ring (232), and the other end of the connecting block (236) is fixedly connected to the first sealing plate (237), and the first sealing plate (237) can be sealed and fitted with the bottom of the first concave bottom (121); The second unlocking assembly (24) comprises a first connecting rod (241), a second sealing plate (242), a vertical slide rail (243), a hinge seat (244), a second connecting rod (245) and a horizontal slide rail (246); the second sealing plate (242) is vertically slidably arranged on the inner side wall of the incubator body (1); a liquid outlet opening is arranged on the outer side wall of the incubator body (1); the second sealing plate (242) can be completely sealed at the liquid outlet opening; the second sealing plate (242) is in communication with the inner side wall of the incubator body (1); The first connecting rod (241) is slidably connected via a vertical slide rail (243), one end of the first connecting rod (241) is hinged to the lifting plate (222), the other end of the first connecting rod (241) is hinged to the second sealing plate (242), the hinge seat (244) is horizontally slidably arranged on the partition (11) via a horizontal slide rail (246), one end of the second connecting rod (245) is hinged to the second sealing plate (242), the other end of the second connecting rod (245) is hinged to the hinge seat (244), and the drain pipe (213) is transmission-connected to the hinge seat (244).

2. The soil remediation bacteria culture box according to claim 1, characterized in that: The temperature control component (32) comprises a bottom electric heating plate (322) and a side electric heating plate (321), wherein the bottom electric heating plate (322) is fixedly mounted on the top of the partition (11), and the side electric heating plate (321) is fixedly mounted on the inner side wall of the upper cavity of the incubator body (1). The disinfection component (33) comprises a first ultraviolet lamp (331) and a second ultraviolet lamp (332), wherein the first ultraviolet lamp (331) is fixedly mounted on the top of the upper cavity of the incubator body (1), and the second ultraviolet lamp (332) is fixedly mounted on the inner side wall of the upper cavity of the incubator body (1). 1), the stirring assembly (34) comprises a stirring shaft (341), a stirring drive motor (342) and a stirring paddle (343), the stirring shaft (341) is vertically arranged inside the incubator body (1), the stirring drive motor (342) is fixedly installed on the top of the incubator body (1), the output end of the stirring drive motor (342) is fixedly connected to the top of the stirring shaft (341), the stirring paddle (343) is arranged inside the incubator (12), and the stirring paddle (343) is fixedly connected to the stirring shaft (341).

3. The soil remediation bacteria culture box according to claim 2, characterized in that: The gas circulation assembly (31) comprises a gas circulation box (311), an air outlet passage (312) and an air inlet passage. The gas circulation box (311) is fixedly mounted on the outer wall of the incubator body (1). One end of the air outlet passage (312) is in communication with the interior of the gas circulation box (311), and the other end of the air outlet passage (312) is in communication with the interior of the upper cavity of the incubator body (1). The air inlet passage comprises a sealing connection ring (313), an air inlet pipe (314) and an air outlet hole (315). The stirring shaft (341) and the stirring paddle (343) are provided with an air inlet passage. The air outlet hole (315) is provided on the stirring paddle (343). The rotatable shaft of the sealing connection ring (313) is connected to the stirring shaft (341). The sealing connection ring (313) is in communication with the air inlet passage. One end of the air inlet pipe (314) is in communication with the gas circulation box (311), and the other end of the air inlet pipe (314) is in communication with the sealing connection ring (313).

4. The soil remediation bacteria culture box according to claim 3, characterized in that: The original strain adding component (41) comprises a strain feeding chamber (411), a feeding connecting pipe (412) and a second electrically controlled valve (413). The strain feeding chamber (411) is mounted on the top of the incubator body (1). The feeding connecting pipe (412) is arranged above the incubator (12). One end of the feeding connecting pipe (412) passes through the top of the incubator body (1) and is fixedly connected to the strain feeding chamber (411). The second electrically controlled valve (413) is mounted on the feeding connecting pipe (412). An air blowing pipe is also arranged inside the strain feeding chamber (411), and one end of the air blowing pipe is connected to the gas circulation box (311).

5. The soil remediation bacteria culture box according to claim 4, characterized in that: The automatic liquid replenishment component (42) comprises a water tank (421), an upper water pipe (422) and a water pump (423); the bottom of the water purification chamber (13) is composed of an inclined surface (131) and a second concave bottom (132); a first water outlet hole (133) is provided on the second concave bottom (132); the water tank (421) is fixedly mounted on the outer side wall of the incubator body (1); the water outlet end of the water tank (421) is in communication with the water purification chamber (13); one end of the upper water pipe (422) is in communication with the interior of the water purification chamber (13) through the first water outlet hole (133); the other end of the upper water pipe (422) is arranged above the incubator (12); the water pump (423) is mounted on the inner side wall of the incubator body (1); and the water pump (423) is mounted on the upper water pipe (422).

6. The soil remediation bacteria culture box according to claim 5, characterized in that: The culture detection mechanism (5) comprises a bottom temperature detection sensor (51), a water layer temperature detection sensor (52) and a liquid level detection float (53); the bottom temperature detection sensor (51) is installed at the bottom of the tank of the culture box (12); the water layer temperature detection sensor (52) is installed in the middle of the culture box (12); and the liquid level detection float (53) is arranged on the liquid surface of the culture box (12).

7. A method for preparing a soil remediation bacterial agent, applied to the soil remediation bacterial culture box according to claim 6, characterized in that: The following steps are involved: S1: During the initial operation, the interior of the incubator body (1) is first disinfected by the first ultraviolet lamp (331) and the second ultraviolet lamp (332). When the initial bacterial strain is added, the initial bacterial strain in the bacterial strain feeding chamber (411) is introduced into the incubator (12) inside the incubator body (1) along the feeding connecting pipe (412). The opening and closing of the feeding connecting pipe (412) is controlled by the second electrically controlled valve (413). When the liquid replenishment operation is required, A culture fluid is provided to the interior of the water purification chamber (13) through a water tank (421), and the culture fluid is irradiated and sterilized by a second ultraviolet lamp (332) inside the water purification chamber (13). After the sterilization is completed, the water pump (423) is started to pump water from the water purification chamber (13) through the upper water pipe (422) into the culture box (12), thereby completing the liquid replenishment operation. The inclined surface (131) and the second concave bottom (132) are arranged to facilitate emptying the water in the water purification chamber (13); S2: The function of cultivating bacterial strains is realized by a culture environment maintaining mechanism (3), and the culture environment maintaining mechanism (3) is used to maintain the oxygen content, temperature, mixing degree of bacterial strains in the culture liquid, etc. in the culture box (12) during the cultivation process; S3: After the bacterial strain culture is completed, the liquid collection operation can be realized through the liquid collection mechanism (2), the lifting drive component (22) drives the first unlocking component (23) and the second unlocking component (24) to move, the first unlocking component (23) opens the liquid outlet end of the culture box (12), and the second unlocking component (24) opens the outer wall of the culture box body (1), and at the same time, the liquid collection component (21) is inserted into the culture box (12) to discharge the cultured bacterial agent in the culture box (12) to the outside; S4: After a certain amount of discharge is stopped, and sufficient mother liquid for subsequent culture is left in the culture box (12), the liquid extraction mechanism (2) is reset, and the automatic liquid replenishment component (42) introduces the culture liquid that has been sterilized in the water purification chamber (13) into the culture box (12), thereby realizing a continuous culture process.

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